JP6477488B2 - 検出装置および当該検出装置を用いた検出方法 - Google Patents
検出装置および当該検出装置を用いた検出方法 Download PDFInfo
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- JP6477488B2 JP6477488B2 JP2015545333A JP2015545333A JP6477488B2 JP 6477488 B2 JP6477488 B2 JP 6477488B2 JP 2015545333 A JP2015545333 A JP 2015545333A JP 2015545333 A JP2015545333 A JP 2015545333A JP 6477488 B2 JP6477488 B2 JP 6477488B2
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Description
実施の形態1では、本発明に係る検出装置であるSPFS装置の一実施の形態について説明する。
実施の形態1の変形例に係るSPFS装置は、ヒートブロックの構成が実施の形態1に係るSPFS装置100と異なる。そこで、主として実施の形態1に係るSPFS装置100と異なる部分について説明する。
実施の形態1のSPFS装置100に使用される変形例に係る検出チップ150’、150”は、金属膜152’の大きさなどが実施の形態1に係る検出チップ150と異なる。そこで、主として実施の形態1に係る検出チップ150と異なる部分について説明する。
実施の形態2に係るSPFS装置200は、試薬保管部250を有する点、試薬保管部250を加熱する第2加熱部230を有する点などにおいて、実施の形態1に係るSPFS装置100と異なる。そこで、主として実施の形態1に係るSPFS装置100と異なる部分について説明する。
図10は、実施の形態2の変形例の検出チップ350の長辺方向の断面図である。図10に示されるように、実施の形態2の変形例の検出チップ350の金属膜152は、成膜面162の外側に延在するように配置されている。この場合、加熱部130のヒートブロック131が金属膜152を直接加熱することができるため、液体貯留部173内の反応場の液体の加熱時間を短縮することができる。
110 励起光照射部
120 光検出部
121 第1レンズ
122 フィルター
123 第2レンズ
124 光センサー
130 加熱部(第1加熱部)
131 ヒートブロック(第1ヒートブロック)
132 熱源(第1熱源)
133 凹部
140,240 制御部
150,350 検出チップ(検出部)
150a ホルダー
151 プリズム
152 金属膜
153 反応部
154,354 基体(第1基体)
161 プリズムの入射面
162 プリズムの成膜面
163 プリズムの出射面
164 プリズムの下面
166 基体の上面
167 基体の側面
168 基体の下面
171 流路溝
172 流路
173 液体貯留部
174 第1貫通孔
175 第2貫通孔
175a 封止シール
176 注入口
177 取出口
230 第2加熱部
231 第2ヒートブロック
232 第2熱源
250 試薬保管部
254 第2基体
255 ウェル
273 検出用ウェル
280 移動部
281 ステージ
282 移動機構
290 送液部
291 ピペット
292 ポンプ
Claims (18)
- 表面プラズモン共鳴を利用して、検体に含まれる被検出物質の存在またはその量を検出するための検出装置であって、
入射面および成膜面を有するプリズムと、前記成膜面上に配置された金属膜と、前記金属膜上に配置された捕捉体と、前記金属膜の前記捕捉体が配置された面と同じ面上に配置され、前記金属膜と共に液体を貯留する液体貯留部を形成する基体と、を有する検出チップを保持するためのホルダーと、
前記入射面に向かって励起光を照射する励起光照射部と、
接触状態または非接触状態で、前記基体、前記プリズムおよび前記金属膜のうち、少なくともいずれかを加熱する加熱部と、を有し、
前記加熱部は、前記励起光照射部から前記入射面までの励起光の光路を避けて配置されており、
前記検出チップは、前記液体貯留部と連通していない、前記液体貯留部に供給される検体または試薬を保管するための試薬保管部をさらに有し、
前記加熱部は、接触状態または非接触状態で、前記試薬保管部をさらに加熱する、
検出装置。 - 前記加熱部は、前記基体の上面、側面および下面のうち、少なくともいずれかを加熱する、請求項1に記載の検出装置。
- 前記加熱部は、前記成膜面に対向する前記プリズムの下面を加熱する、請求項1に記載の検出装置。
- 前記加熱部は、前記基体の下面、前記プリズムの入射面、前記成膜面に対向する前記プリズムの下面、および前記プリズムの前記成膜面で反射した励起光を出射させる出射面を加熱する、請求項1に記載の検出装置。
- 前記加熱部は、誘導加熱によって前記金属膜を加熱する、請求項1に記載の検出装置。
- 前記金属膜の前記プリズムと対向しない面の近傍から出射される光を検出する光検出部をさらに有する、請求項1に記載の検出装置。
- 前記プリズムの前記成膜面で反射した励起光を検出する光検出部をさらに有する、請求項1に記載の検出装置。
- 前記金属膜は、前記成膜面の外側に延在するように配置されており、
前記加熱部は、前記金属膜を加熱する、
請求項1に記載の検出装置。 - 前記基体は、前記液体貯留部に前記検体を注入するための注入口を有し、
前記注入口は、金属製またはカーボン製の封止シールで封止されており、
前記加熱部は、前記封止シールを加熱することで前記基体を加熱する、
請求項1に記載の検出装置。 - 表面プラズモン共鳴を利用して、検体に含まれる被検出物質の存在またはその量を検出するための検出方法であって、
入射面および成膜面を有するプリズムと、前記成膜面上に配置された金属膜と、前記金属膜上に固定された捕捉体と、前記金属膜の前記捕捉体が配置された面と同じ面上に配置された、前記金属膜と共に前記検体を貯留する液体貯留部を形成する基体と、を有する検出部を含む検出チップを準備する工程と、
前記検出チップの前記基体、前記プリズムおよび前記金属膜のうち、少なくともいずれかを加熱する工程と、
前記検体を前記捕捉体に接触させて、前記検体に含まれる被検出物質と前記捕捉体とを結合させる工程と、
前記プリズムと前記金属膜との界面において全反射するように、前記プリズム側から前記金属膜に励起光を照射する工程と、
を有し、
前記検出チップは、前記液体貯留部と連通していない、前記液体貯留部に供給される検体または試料を保管するための試薬保管部をさらに有し、
前記加熱する工程では、接触状態または非接触状態で、前記試薬保管部をさらに加熱する、
検出方法。 - 前記加熱する工程では、34〜40℃に加熱する、請求項10に記載の検出方法。
- 前記加熱する工程では、前記基体の上面、側面および下面のうち、少なくともいずれかを加熱する、請求項10に記載の検出方法。
- 前記加熱する工程では、前記成膜面に対向する前記プリズムの下面を加熱する、請求項10に記載の検出方法。
- 前記加熱する工程では、前記基体の下面、前記プリズムの入射面、前記成膜面に対向する前記プリズムの下面、および前記成膜面で反射した励起光を出射させる出射面を加熱する、請求項10に記載の検出方法。
- 前記加熱する工程では、前記検出部を34〜40℃に加熱するとともに、前記試薬保管部を前記検出部の温度未満の温度に、接触状態または非接触状態で加熱する、
請求項10に記載の検出方法。 - 前記加熱部は、誘導加熱によって前記金属膜を加熱する、請求項10に記載の検出方法。
- 前記金属膜の前記プリズムと対向しない面の近傍から出射される光を検出する工程をさらに有する、請求項10に記載の検出方法。
- 前記プリズムの前記成膜面で反射した励起光を検出する工程をさらに有する、請求項10に記載の検出方法。
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