JP4469655B2 - 核酸抽出装置 - Google Patents
核酸抽出装置 Download PDFInfo
- Publication number
- JP4469655B2 JP4469655B2 JP2004148365A JP2004148365A JP4469655B2 JP 4469655 B2 JP4469655 B2 JP 4469655B2 JP 2004148365 A JP2004148365 A JP 2004148365A JP 2004148365 A JP2004148365 A JP 2004148365A JP 4469655 B2 JP4469655 B2 JP 4469655B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- cartridge
- acid extraction
- liquid
- nozzle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims description 140
- 102000039446 nucleic acids Human genes 0.000 title claims description 140
- 108020004707 nucleic acids Proteins 0.000 title claims description 140
- 238000000605 extraction Methods 0.000 title claims description 84
- 239000007788 liquid Substances 0.000 claims description 150
- 230000007246 mechanism Effects 0.000 claims description 48
- 238000004140 cleaning Methods 0.000 claims description 46
- 238000011084 recovery Methods 0.000 claims description 37
- 238000005406 washing Methods 0.000 claims description 12
- 239000012535 impurity Substances 0.000 claims description 4
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- 239000010408 film Substances 0.000 description 37
- 239000002699 waste material Substances 0.000 description 30
- 238000000034 method Methods 0.000 description 25
- 229920002301 cellulose acetate Polymers 0.000 description 23
- 239000012488 sample solution Substances 0.000 description 23
- 239000000203 mixture Substances 0.000 description 22
- 229920000642 polymer Polymers 0.000 description 22
- SMEGJBVQLJJKKX-HOTMZDKISA-N [(2R,3S,4S,5R,6R)-5-acetyloxy-3,4,6-trihydroxyoxan-2-yl]methyl acetate Chemical compound CC(=O)OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)O)OC(=O)C)O)O SMEGJBVQLJJKKX-HOTMZDKISA-N 0.000 description 18
- 229940081735 acetylcellulose Drugs 0.000 description 18
- 239000011368 organic material Substances 0.000 description 18
- 239000000243 solution Substances 0.000 description 17
- 229920000578 graft copolymer Polymers 0.000 description 16
- 238000007127 saponification reaction Methods 0.000 description 14
- 239000000463 material Substances 0.000 description 13
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 12
- 229920002284 Cellulose triacetate Polymers 0.000 description 11
- NNLVGZFZQQXQNW-ADJNRHBOSA-N [(2r,3r,4s,5r,6s)-4,5-diacetyloxy-3-[(2s,3r,4s,5r,6r)-3,4,5-triacetyloxy-6-(acetyloxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6s)-4,5,6-triacetyloxy-2-(acetyloxymethyl)oxan-3-yl]oxyoxan-2-yl]methyl acetate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](OC(C)=O)[C@H]1OC(C)=O)O[C@H]1[C@@H]([C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1)OC(C)=O)COC(=O)C)[C@@H]1[C@@H](COC(C)=O)O[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O NNLVGZFZQQXQNW-ADJNRHBOSA-N 0.000 description 11
- 229920005994 diacetyl cellulose Polymers 0.000 description 11
- 229910010272 inorganic material Inorganic materials 0.000 description 11
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- -1 polyoxyethylene Polymers 0.000 description 11
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 10
- 239000000178 monomer Substances 0.000 description 10
- 230000008569 process Effects 0.000 description 10
- 150000001875 compounds Chemical class 0.000 description 9
- 125000000524 functional group Chemical group 0.000 description 9
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000011248 coating agent Substances 0.000 description 6
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- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 4
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 4
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 4
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 4
- 229920002845 Poly(methacrylic acid) Polymers 0.000 description 4
- 239000004372 Polyvinyl alcohol Substances 0.000 description 4
- 229920002125 Sokalan® Polymers 0.000 description 4
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 230000002093 peripheral effect Effects 0.000 description 4
- 239000004584 polyacrylic acid Substances 0.000 description 4
- 229920002451 polyvinyl alcohol Polymers 0.000 description 4
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 4
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 4
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- 239000000919 ceramic Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 229920000620 organic polymer Polymers 0.000 description 3
- 230000000379 polymerizing effect Effects 0.000 description 3
- 239000004627 regenerated cellulose Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000003028 elevating effect Effects 0.000 description 2
- 125000003700 epoxy group Chemical group 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 150000004676 glycans Polymers 0.000 description 2
- 238000010559 graft polymerization reaction Methods 0.000 description 2
- 125000001905 inorganic group Chemical group 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- IQPQWNKOIGAROB-UHFFFAOYSA-N isocyanate group Chemical group [N-]=C=O IQPQWNKOIGAROB-UHFFFAOYSA-N 0.000 description 2
- 150000004804 polysaccharides Polymers 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- QRIMLDXJAPZHJE-UHFFFAOYSA-N 2,3-dihydroxypropyl 2-methylprop-2-enoate Chemical compound CC(=C)C(=O)OCC(O)CO QRIMLDXJAPZHJE-UHFFFAOYSA-N 0.000 description 1
- OMIGHNLMNHATMP-UHFFFAOYSA-N 2-hydroxyethyl prop-2-enoate Chemical compound OCCOC(=O)C=C OMIGHNLMNHATMP-UHFFFAOYSA-N 0.000 description 1
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 1
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical group OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 125000003647 acryloyl group Chemical group O=C([*])C([H])=C([H])[H] 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 125000004093 cyano group Chemical group *C#N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 125000006353 oxyethylene group Chemical group 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000003566 sealing material Substances 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 125000000542 sulfonic acid group Chemical group 0.000 description 1
- 125000005369 trialkoxysilyl group Chemical group 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004148365A JP4469655B2 (ja) | 2004-05-18 | 2004-05-18 | 核酸抽出装置 |
| PCT/JP2005/009308 WO2005111210A1 (en) | 2004-05-18 | 2005-05-17 | Method for extracting nucleic acid and nucleic acid-extracting apparatus |
| US11/596,666 US20070244314A1 (en) | 2004-05-18 | 2005-05-17 | Method For Extracting Nucleic Acid And Nucleic Acid-Extracting Apparatus |
| EP05740957A EP1747271A4 (en) | 2004-05-18 | 2005-05-17 | PROCESS FOR EXTRACTING NUCLEIC ACID AND APPARATUS FOR EXTRACTING NUCLEIC ACID |
| CNA2005800161591A CN101389757A (zh) | 2004-05-18 | 2005-05-17 | 核酸提取方法及核酸提取装置 |
| CN2010102327895A CN101886037B (zh) | 2004-05-18 | 2005-05-17 | 核酸提取方法及核酸提取装置 |
| US12/838,214 US20100276357A1 (en) | 2004-05-18 | 2010-07-16 | Method for extracting nucleic acid and nucleic acid-extracting apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004148365A JP4469655B2 (ja) | 2004-05-18 | 2004-05-18 | 核酸抽出装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005328730A JP2005328730A (ja) | 2005-12-02 |
| JP4469655B2 true JP4469655B2 (ja) | 2010-05-26 |
Family
ID=35483782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004148365A Expired - Lifetime JP4469655B2 (ja) | 2004-05-18 | 2004-05-18 | 核酸抽出装置 |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP4469655B2 (zh) |
| CN (1) | CN101389757A (zh) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4478588B2 (ja) * | 2005-01-31 | 2010-06-09 | 富士フイルム株式会社 | 特定物質回収装置及びこれを用いた核酸抽出装置 |
| JP4431508B2 (ja) | 2005-02-21 | 2010-03-17 | 富士フイルム株式会社 | 核酸抽出装置用のカートリッジ保持機構 |
| JP4431509B2 (ja) | 2005-02-21 | 2010-03-17 | 富士フイルム株式会社 | 核酸抽出装置用のカートリッジ保持機構 |
| US9428746B2 (en) | 2007-10-31 | 2016-08-30 | Akonni Biosystems, Inc. | Method and kit for purifying nucleic acids |
| CA2883463A1 (en) * | 2012-08-28 | 2014-03-06 | Akonni Biosystems Inc. | Method and kit for purifying nucleic acids |
| CN117467658A (zh) * | 2022-07-22 | 2024-01-30 | 友康生物科技(北京)股份有限公司 | 核酸提取和检测的方法及装置 |
-
2004
- 2004-05-18 JP JP2004148365A patent/JP4469655B2/ja not_active Expired - Lifetime
-
2005
- 2005-05-17 CN CNA2005800161591A patent/CN101389757A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN101389757A (zh) | 2009-03-18 |
| JP2005328730A (ja) | 2005-12-02 |
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