JP4468594B2 - 固体相内の分析物質を電気化学的に定量分析するためのシステム - Google Patents
固体相内の分析物質を電気化学的に定量分析するためのシステム Download PDFInfo
- Publication number
- JP4468594B2 JP4468594B2 JP2000618714A JP2000618714A JP4468594B2 JP 4468594 B2 JP4468594 B2 JP 4468594B2 JP 2000618714 A JP2000618714 A JP 2000618714A JP 2000618714 A JP2000618714 A JP 2000618714A JP 4468594 B2 JP4468594 B2 JP 4468594B2
- Authority
- JP
- Japan
- Prior art keywords
- test strip
- affinity chromatographic
- chromatographic test
- test
- substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000004445 quantitative analysis Methods 0.000 title claims abstract description 27
- 238000000840 electrochemical analysis Methods 0.000 title claims abstract description 15
- 239000007790 solid phase Substances 0.000 title abstract description 31
- 238000012360 testing method Methods 0.000 claims abstract description 209
- 239000000126 substance Substances 0.000 claims abstract description 82
- 239000012491 analyte Substances 0.000 claims abstract description 68
- 238000000034 method Methods 0.000 claims abstract description 48
- 238000004458 analytical method Methods 0.000 claims abstract description 40
- 239000000463 material Substances 0.000 claims abstract description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 6
- 238000002372 labelling Methods 0.000 claims description 53
- 229910052751 metal Inorganic materials 0.000 claims description 38
- 239000002184 metal Substances 0.000 claims description 38
- 239000003446 ligand Substances 0.000 claims description 27
- 239000002609 medium Substances 0.000 claims description 13
- 239000012501 chromatography medium Substances 0.000 claims description 8
- 239000002502 liposome Substances 0.000 claims description 6
- 229910052802 copper Inorganic materials 0.000 claims description 5
- 229910052738 indium Inorganic materials 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 238000001042 affinity chromatography Methods 0.000 claims description 3
- 229910052692 Dysprosium Inorganic materials 0.000 claims description 2
- 229910052691 Erbium Inorganic materials 0.000 claims description 2
- 229910052693 Europium Inorganic materials 0.000 claims description 2
- 229910052688 Gadolinium Inorganic materials 0.000 claims description 2
- 229910052689 Holmium Inorganic materials 0.000 claims description 2
- 229910052779 Neodymium Inorganic materials 0.000 claims description 2
- 229910052778 Plutonium Inorganic materials 0.000 claims description 2
- 229910052777 Praseodymium Inorganic materials 0.000 claims description 2
- 229910052772 Samarium Inorganic materials 0.000 claims description 2
- 229910052771 Terbium Inorganic materials 0.000 claims description 2
- 229910052776 Thorium Inorganic materials 0.000 claims description 2
- 229910052770 Uranium Inorganic materials 0.000 claims description 2
- 229910052782 aluminium Inorganic materials 0.000 claims description 2
- 229910052787 antimony Inorganic materials 0.000 claims description 2
- 229910052793 cadmium Inorganic materials 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 229910052804 chromium Inorganic materials 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- 229910052745 lead Inorganic materials 0.000 claims description 2
- 229910052749 magnesium Inorganic materials 0.000 claims description 2
- 229910052748 manganese Inorganic materials 0.000 claims description 2
- 229910052750 molybdenum Inorganic materials 0.000 claims description 2
- 229910052759 nickel Inorganic materials 0.000 claims description 2
- 229910052763 palladium Inorganic materials 0.000 claims description 2
- 229910052702 rhenium Inorganic materials 0.000 claims description 2
- 229910052703 rhodium Inorganic materials 0.000 claims description 2
- 229910052706 scandium Inorganic materials 0.000 claims description 2
- 229910052713 technetium Inorganic materials 0.000 claims description 2
- 229910052718 tin Inorganic materials 0.000 claims description 2
- 229910052719 titanium Inorganic materials 0.000 claims description 2
- 229910052721 tungsten Inorganic materials 0.000 claims description 2
- 229910052720 vanadium Inorganic materials 0.000 claims description 2
- 229910052727 yttrium Inorganic materials 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- 229910052726 zirconium Inorganic materials 0.000 claims description 2
- 230000003100 immobilizing effect Effects 0.000 claims 2
- 229910052684 Cerium Inorganic materials 0.000 claims 1
- 239000011230 binding agent Substances 0.000 claims 1
- 229910052746 lanthanum Inorganic materials 0.000 claims 1
- 239000000523 sample Substances 0.000 abstract description 63
- 239000012530 fluid Substances 0.000 abstract description 23
- 102000004169 proteins and genes Human genes 0.000 abstract description 15
- 108090000623 proteins and genes Proteins 0.000 abstract description 15
- 230000007704 transition Effects 0.000 abstract description 13
- 241000894007 species Species 0.000 abstract description 12
- 238000003968 anodic stripping voltammetry Methods 0.000 abstract description 10
- 239000012488 sample solution Substances 0.000 abstract description 9
- 230000003993 interaction Effects 0.000 abstract description 7
- 230000002596 correlated effect Effects 0.000 abstract description 5
- 241000700605 Viruses Species 0.000 abstract description 4
- -1 antibodies Proteins 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 4
- 229940079593 drug Drugs 0.000 abstract description 4
- 239000002245 particle Substances 0.000 abstract description 4
- 102000004190 Enzymes Human genes 0.000 abstract description 3
- 108090000790 Enzymes Proteins 0.000 abstract description 3
- 108020004707 nucleic acids Proteins 0.000 abstract description 3
- 102000039446 nucleic acids Human genes 0.000 abstract description 3
- 150000007523 nucleic acids Chemical class 0.000 abstract description 3
- 210000002421 cell wall Anatomy 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 150000004676 glycans Chemical class 0.000 abstract description 2
- 239000005556 hormone Substances 0.000 abstract description 2
- 229940088597 hormone Drugs 0.000 abstract description 2
- 229920001282 polysaccharide Polymers 0.000 abstract description 2
- 239000005017 polysaccharide Substances 0.000 abstract description 2
- 150000003384 small molecules Chemical class 0.000 abstract description 2
- 239000003053 toxin Substances 0.000 abstract description 2
- 231100000765 toxin Toxicity 0.000 abstract description 2
- 108700012359 toxins Proteins 0.000 abstract description 2
- 238000003556 assay Methods 0.000 abstract 2
- 238000012544 monitoring process Methods 0.000 abstract 2
- 206010060862 Prostate cancer Diseases 0.000 abstract 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 abstract 1
- 230000001773 anti-convulsant effect Effects 0.000 abstract 1
- 229940125681 anticonvulsant agent Drugs 0.000 abstract 1
- 239000001961 anticonvulsive agent Substances 0.000 abstract 1
- 238000002820 assay format Methods 0.000 abstract 1
- 239000013060 biological fluid Substances 0.000 abstract 1
- 238000012875 competitive assay Methods 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 230000037361 pathway Effects 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 30
- 239000000243 solution Substances 0.000 description 24
- 238000005259 measurement Methods 0.000 description 17
- 230000004044 response Effects 0.000 description 17
- 108091034117 Oligonucleotide Proteins 0.000 description 16
- 239000000376 reactant Substances 0.000 description 16
- 239000007788 liquid Substances 0.000 description 10
- 239000012528 membrane Substances 0.000 description 10
- 229910021645 metal ion Inorganic materials 0.000 description 10
- 238000004094 preconcentration Methods 0.000 description 10
- 230000008859 change Effects 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 238000010998 test method Methods 0.000 description 9
- 150000002500 ions Chemical class 0.000 description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 7
- JCXGWMGPZLAOME-UHFFFAOYSA-N bismuth atom Chemical compound [Bi] JCXGWMGPZLAOME-UHFFFAOYSA-N 0.000 description 7
- 229910052799 carbon Inorganic materials 0.000 description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 6
- 229910052797 bismuth Inorganic materials 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- QPCDCPDFJACHGM-UHFFFAOYSA-N N,N-bis{2-[bis(carboxymethyl)amino]ethyl}glycine Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(=O)O)CCN(CC(O)=O)CC(O)=O QPCDCPDFJACHGM-UHFFFAOYSA-N 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- 239000002250 absorbent Substances 0.000 description 4
- 230000002745 absorbent Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 230000002860 competitive effect Effects 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- 230000000875 corresponding effect Effects 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 229910052753 mercury Inorganic materials 0.000 description 4
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 4
- 229960003330 pentetic acid Drugs 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- 102000036675 Myoglobin Human genes 0.000 description 3
- 108010062374 Myoglobin Proteins 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 229910001451 bismuth ion Inorganic materials 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 description 3
- 239000004816 latex Substances 0.000 description 3
- 229920000126 latex Polymers 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 229920000728 polyester Polymers 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 238000003950 stripping voltammetry Methods 0.000 description 3
- YOETUEMZNOLGDB-UHFFFAOYSA-N 2-methylpropyl carbonochloridate Chemical compound CC(C)COC(Cl)=O YOETUEMZNOLGDB-UHFFFAOYSA-N 0.000 description 2
- 229910000497 Amalgam Inorganic materials 0.000 description 2
- 229920002799 BoPET Polymers 0.000 description 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 2
- 229910021607 Silver chloride Inorganic materials 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000012490 blank solution Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 230000009918 complex formation Effects 0.000 description 2
- 239000004020 conductor Substances 0.000 description 2
- 229910001431 copper ion Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002848 electrochemical method Methods 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 239000011152 fibreglass Substances 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 238000009597 pregnancy test Methods 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 238000005353 urine analysis Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000212892 Chelon Species 0.000 description 1
- 206010013654 Drug abuse Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000015215 Stem Cell Factor Human genes 0.000 description 1
- 108010039445 Stem Cell Factor Proteins 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 102000023732 binding proteins Human genes 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 150000004696 coordination complex Chemical class 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- 239000011532 electronic conductor Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 229910052732 germanium Inorganic materials 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000010416 ion conductor Substances 0.000 description 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 1
- 125000005647 linker group Chemical group 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 150000003071 polychlorinated biphenyls Chemical group 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 208000011117 substance-related disease Diseases 0.000 description 1
- 229910052714 tellurium Inorganic materials 0.000 description 1
- 229910052716 thallium Inorganic materials 0.000 description 1
- BKVIYDNLLOSFOA-UHFFFAOYSA-N thallium Chemical compound [Tl] BKVIYDNLLOSFOA-UHFFFAOYSA-N 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
- G01N33/54388—Immunochromatographic test strips based on lateral flow
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/30—Electrochemically active labels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/97—Test strip or test slide
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/305,771 US6485983B1 (en) | 1999-05-05 | 1999-05-05 | System for electrochemical quantitative analysis of analytes within a solid phase and affinity chromatographic test strip |
| US09/305,771 | 1999-05-05 | ||
| PCT/US2000/012099 WO2000070327A1 (en) | 1999-05-05 | 2000-05-05 | System for electrochemical quantitative analysis of analytes within a solid phase |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003519362A JP2003519362A (ja) | 2003-06-17 |
| JP2003519362A5 JP2003519362A5 (enExample) | 2007-06-28 |
| JP4468594B2 true JP4468594B2 (ja) | 2010-05-26 |
Family
ID=23182278
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000618714A Expired - Lifetime JP4468594B2 (ja) | 1999-05-05 | 2000-05-05 | 固体相内の分析物質を電気化学的に定量分析するためのシステム |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US6485983B1 (enExample) |
| EP (1) | EP1190231B1 (enExample) |
| JP (1) | JP4468594B2 (enExample) |
| CN (1) | CN100362335C (enExample) |
| AT (1) | ATE311591T1 (enExample) |
| AU (1) | AU781131C (enExample) |
| CA (1) | CA2373246A1 (enExample) |
| DE (1) | DE60024448T2 (enExample) |
| ES (1) | ES2254175T3 (enExample) |
| HK (1) | HK1046548A1 (enExample) |
| MX (1) | MXPA01011212A (enExample) |
| WO (1) | WO2000070327A1 (enExample) |
| ZA (1) | ZA200108793B (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110456030A (zh) * | 2019-08-28 | 2019-11-15 | 国网山东省电力公司电力科学研究院 | 一种燃煤电厂脱硫石膏中Hg、Pb的赋存形态分析方法 |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6485983B1 (en) * | 1999-05-05 | 2002-11-26 | Intec Science, Inc. | System for electrochemical quantitative analysis of analytes within a solid phase and affinity chromatographic test strip |
| FR2810739B1 (fr) * | 2000-06-26 | 2007-01-26 | Centre Nat Rech Scient | Immunodosage electrochimiques a l'aide de marqueurs metalliques colloidaux |
| US6887714B2 (en) * | 2000-10-16 | 2005-05-03 | Board Of Trustees Of The University Of Arkansas, N.A. | Microvolume immunoabsorbant assays with amplified electrochemical detection |
| US7456028B2 (en) * | 2000-10-16 | 2008-11-25 | Board Of Trustees Of The University Of Arkansas, N.A. | Electrochemical method for detecting water born pathogens |
| US20040121350A1 (en) * | 2002-12-24 | 2004-06-24 | Biosite Incorporated | System and method for identifying a panel of indicators |
| US20040203083A1 (en) * | 2001-04-13 | 2004-10-14 | Biosite, Inc. | Use of thrombus precursor protein and monocyte chemoattractant protein as diagnostic and prognostic indicators in vascular diseases |
| US20040253637A1 (en) * | 2001-04-13 | 2004-12-16 | Biosite Incorporated | Markers for differential diagnosis and methods of use thereof |
| US20040126767A1 (en) * | 2002-12-27 | 2004-07-01 | Biosite Incorporated | Method and system for disease detection using marker combinations |
| US7713705B2 (en) | 2002-12-24 | 2010-05-11 | Biosite, Inc. | Markers for differential diagnosis and methods of use thereof |
| US8676897B1 (en) * | 2001-11-30 | 2014-03-18 | Hewlett-Packard Development Company, L.P. | N-way interactive communication using hand held computers |
| US20030153094A1 (en) * | 2002-02-13 | 2003-08-14 | Board Of Trustees Of Michigan State University | Conductimetric biosensor device, method and system |
| USD497673S1 (en) | 2002-08-22 | 2004-10-26 | Inverness Medical Switzerland Gmbh | Testing device |
| USD497999S1 (en) | 2002-08-22 | 2004-11-02 | Inverness Medical Switzerland Gmbh | Testing device |
| FR2844052B1 (fr) * | 2002-08-28 | 2005-07-01 | Commissariat Energie Atomique | Dispositif de mesure de l'activite electrique d'elements biologiques et ses applications |
| US20050058689A1 (en) | 2003-07-03 | 2005-03-17 | Reactive Surfaces, Ltd. | Antifungal paints and coatings |
| US7176036B2 (en) * | 2002-09-20 | 2007-02-13 | Arrowhead Center, Inc. | Electroactive microspheres and methods |
| US20040118704A1 (en) * | 2002-12-19 | 2004-06-24 | Yi Wang | Analyte test intrument having improved versatility |
| EP1588159A4 (en) * | 2002-12-24 | 2008-03-12 | Biosite Inc | METHOD AND SYSTEM FOR DETECTING DISORDERS BY MARKER COMBINATIONS |
| CN100504372C (zh) * | 2003-02-14 | 2009-06-24 | 爱科来株式会社 | 具有把手部的分析用具 |
| US7691255B2 (en) * | 2004-05-06 | 2010-04-06 | Panasonic Corporation | Sensor, measuring device, and measuring method |
| CA2568252A1 (en) * | 2004-06-02 | 2005-12-15 | Relia Diagnostic Systems, Llc | Quantitative lateral flow system and assay |
| CN101031798B (zh) | 2004-07-29 | 2012-06-27 | 瑞莱诊断体系有限公司 | 侧向流系统和测定 |
| EP1637882B1 (de) | 2004-09-09 | 2007-08-08 | Analyticon Biotechnologies AG | Lateralflussmessvorrichtung und Messverfahren für Analyten |
| US20060281193A1 (en) * | 2005-06-09 | 2006-12-14 | Petrilla John F | Non-optical reading of test zones |
| US20060286006A1 (en) * | 2005-06-21 | 2006-12-21 | Mcdaniel C S | Method and apparatus for the treatment of fluid waste streams |
| EP2195446A4 (en) * | 2007-09-17 | 2011-06-22 | Red Ivory Llc | Self-activating signal-producing detection devices and methods |
| US8388904B1 (en) | 2008-12-22 | 2013-03-05 | Reactive Surfaces, Ltd., Llp | Equipment decontamination system and method |
| CN101458223B (zh) * | 2008-12-26 | 2012-01-25 | 江南大学 | 一种微囊藻毒素-lr定量快速检测传感器的制备及应用 |
| TW201024728A (en) * | 2008-12-31 | 2010-07-01 | Univ Chung Yuan Christian | A lateral-flow device and method to enhance the detection signal for charged molecular targets |
| EP2418485B1 (en) | 2009-04-09 | 2016-06-22 | Hitachi Chemical Company, Ltd. | Detector and detection method |
| KR101115014B1 (ko) * | 2009-07-17 | 2012-03-06 | 바디텍메드 주식회사 | 핵산 검출용 크로마토그래피 시스템 |
| CN102230939B (zh) * | 2011-03-29 | 2013-08-28 | 中国人民解放军第三军医大学第三附属医院 | 一种检测多种蛋白的电化学免疫分析方法 |
| EP2602620A1 (en) * | 2011-12-07 | 2013-06-12 | Nxp B.V. | An electronic lateral flow test arrangement and method |
| US9335290B2 (en) | 2011-12-23 | 2016-05-10 | Abbott Point Of Care, Inc. | Integrated test device for optical and electrochemical assays |
| EP3447499A1 (en) | 2011-12-23 | 2019-02-27 | Abbott Point of Care Inc. | Optical assay device with pneumatic sample actuation |
| DE102012101254A1 (de) * | 2012-02-16 | 2013-08-22 | Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG | Messanordnung und Verfahren zur Erfassung einer Analytkonzentration in einem Messmedium |
| FI126484B (en) * | 2013-12-03 | 2017-01-13 | Goodwiller Oy | Disposable test strip device for detecting an analyte in a body fluid sample |
| CN106999929A (zh) * | 2014-09-11 | 2017-08-01 | 克忧公司 | 用于检测和量化分析物的系统和方法 |
| BR112017006898A2 (pt) * | 2014-10-07 | 2018-03-27 | 3M Innovative Properties Co | método para detecção de um micro-organismo alvo, dispositivo e kit |
| JP6628794B2 (ja) * | 2014-10-07 | 2020-01-15 | スリーエム イノベイティブ プロパティズ カンパニー | クロマトグラフ濃縮を使用する微生物の検出方法 |
| CN106324064A (zh) * | 2015-06-23 | 2017-01-11 | 中国科学院上海应用物理研究所 | 电化学生物传感系统 |
| CN105424452A (zh) * | 2015-11-04 | 2016-03-23 | 浙江理工大学 | 一种针对丝织品文物进行锶同位素检测的除杂方法 |
| CN105651739B (zh) * | 2016-03-01 | 2019-01-08 | 浙江大学 | 基于溶出伏安的多种重金属离子纳米光学检测装置及方法 |
| CN106501507B (zh) * | 2016-09-20 | 2018-01-12 | 北京化工大学 | 离子浓度极化效应icp免疫检测侧向流层析纸条 |
| CN106706902B (zh) * | 2016-11-18 | 2018-12-18 | 百奥森（江苏）食品安全科技有限公司 | 一种猪圆环病毒抗体快速金标检测卡 |
| EP3379250A1 (en) * | 2017-03-20 | 2018-09-26 | Bundesrepublik Deutschland, vertreten durch den Bundesminister für Wirtschaft und Energie | Indicator release system for the detection of an analyte in a foodstuff, test strip therefor, and analysis method |
| CN108663513B (zh) * | 2018-04-20 | 2019-08-20 | 江南大学 | 一种降低侧流层析试纸检测限的方法 |
| WO2020037078A1 (en) * | 2018-08-14 | 2020-02-20 | Sensor-Kinesis Corporation | Chelator-coated field effect transistor and devices and methods using same |
| CN113316716B (zh) * | 2019-01-11 | 2025-05-13 | 弗雷德传感科技公司 | 用于伏安检测的系统和方法 |
| CN110243919A (zh) * | 2019-05-14 | 2019-09-17 | 中国石油天然气股份有限公司 | 一种利用钒同位素对油气成因与来源进行分析的方法 |
| JP6714256B1 (ja) * | 2019-12-18 | 2020-06-24 | 株式会社イムノセンス | 電気化学法ラテラルフロー式免疫検査方法とそのセンサーおよびその製造方法 |
| CN116157679B (zh) * | 2020-09-16 | 2025-01-10 | 沃特世科技公司 | 用于惰性液相色谱系统和柱的系统适用性测试的化合物 |
| CA3264987A1 (en) * | 2022-08-18 | 2024-02-22 | Eye3Concepts Inc | METHOD FOR DETECTING AN ANALYTE USING MACHINE LEARNING |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4233144A (en) * | 1979-04-16 | 1980-11-11 | Technicon Instruments Corporation | Electrode for voltammetric immunoassay |
| US5591581A (en) * | 1986-04-30 | 1997-01-07 | Igen, Inc. | Electrochemiluminescent rhenium moieties and methods for their use |
| US5389523A (en) * | 1988-05-31 | 1995-02-14 | The United States Of Americas, As Represented By The Secretary Of Commerce | Liposome immunoanalysis by flow injection assay |
| FR2692357A1 (fr) * | 1992-06-12 | 1993-12-17 | Centre Nat Rech Scient | Antigène marqué par un système redox, procédé de dosage immunologique avec détection électrochimique et kit de dosage. |
| FR2710152B1 (fr) * | 1993-09-17 | 1995-12-01 | Centre Nat Rech Scient | Procédé de dosage d'une enzyme et procédé de dosage immunoenzymatique d'un antigène utilisant une électrode modifiée et kit de dosage. |
| US5756362A (en) * | 1993-10-12 | 1998-05-26 | Cornell Research Foundation, Inc. | Liposome-enhanced immunoaggregation assay and test device |
| US5789154A (en) * | 1993-10-12 | 1998-08-04 | Cornell Research Foundation, Inc. | Liposome-enhanced immunoassay and test device |
| GB9402661D0 (en) * | 1994-02-11 | 1994-04-06 | Ecossensors Ltd | Improvements in and relating to detection of lead in blood |
| US5753517A (en) * | 1996-03-29 | 1998-05-19 | University Of British Columbia | Quantitative immunochromatographic assays |
| US5958791A (en) * | 1996-09-27 | 1999-09-28 | Innovative Biotechnologies, Inc. | Interdigitated electrode arrays for liposome-enhanced immunoassay and test device |
| US6485983B1 (en) * | 1999-05-05 | 2002-11-26 | Intec Science, Inc. | System for electrochemical quantitative analysis of analytes within a solid phase and affinity chromatographic test strip |
-
1999
- 1999-05-05 US US09/305,771 patent/US6485983B1/en not_active Expired - Fee Related
-
2000
- 2000-05-05 US US09/701,922 patent/US6713308B1/en not_active Expired - Fee Related
- 2000-05-05 JP JP2000618714A patent/JP4468594B2/ja not_active Expired - Lifetime
- 2000-05-05 CN CNB008072248A patent/CN100362335C/zh not_active Expired - Fee Related
- 2000-05-05 AU AU48181/00A patent/AU781131C/en not_active Ceased
- 2000-05-05 DE DE60024448T patent/DE60024448T2/de not_active Expired - Lifetime
- 2000-05-05 EP EP00930343A patent/EP1190231B1/en not_active Expired - Lifetime
- 2000-05-05 ES ES00930343T patent/ES2254175T3/es not_active Expired - Lifetime
- 2000-05-05 AT AT00930343T patent/ATE311591T1/de not_active IP Right Cessation
- 2000-05-05 HK HK02106882.6A patent/HK1046548A1/zh unknown
- 2000-05-05 WO PCT/US2000/012099 patent/WO2000070327A1/en not_active Ceased
- 2000-05-05 CA CA002373246A patent/CA2373246A1/en not_active Abandoned
- 2000-05-05 MX MXPA01011212A patent/MXPA01011212A/es active IP Right Grant
-
2001
- 2001-10-25 ZA ZA200108793A patent/ZA200108793B/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110456030A (zh) * | 2019-08-28 | 2019-11-15 | 国网山东省电力公司电力科学研究院 | 一种燃煤电厂脱硫石膏中Hg、Pb的赋存形态分析方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU781131B2 (en) | 2005-05-05 |
| ZA200108793B (en) | 2002-05-22 |
| CA2373246A1 (en) | 2000-11-23 |
| JP2003519362A (ja) | 2003-06-17 |
| WO2000070327A1 (en) | 2000-11-23 |
| AU781131C (en) | 2006-08-17 |
| EP1190231B1 (en) | 2005-11-30 |
| ATE311591T1 (de) | 2005-12-15 |
| US6713308B1 (en) | 2004-03-30 |
| CN1350638A (zh) | 2002-05-22 |
| DE60024448T2 (de) | 2006-08-24 |
| AU4818100A (en) | 2000-12-05 |
| CN100362335C (zh) | 2008-01-16 |
| EP1190231A1 (en) | 2002-03-27 |
| EP1190231A4 (en) | 2003-01-02 |
| ES2254175T3 (es) | 2006-06-16 |
| DE60024448D1 (de) | 2006-01-05 |
| MXPA01011212A (es) | 2003-07-14 |
| HK1046548A1 (zh) | 2003-01-17 |
| US6485983B1 (en) | 2002-11-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4468594B2 (ja) | 固体相内の分析物質を電気化学的に定量分析するためのシステム | |
| EP2986987B1 (en) | Electrochemical lateral flow bioassay and biosensor | |
| KR0156176B1 (ko) | 전기화학식 면역 바이오센서 | |
| Díaz‐González et al. | Recent advances in electrochemical enzyme immunoassays | |
| RU2423073C2 (ru) | Микрофлюидные устройства и способы их подготовки и применения | |
| EP2318837B1 (en) | Enhanced immunoassay sensor | |
| US5149629A (en) | Coulometric assay system | |
| JP5036867B2 (ja) | バイオセンサ | |
| US20060141469A1 (en) | Multi-layered electrochemical microfluidic sensor comprising reagent on porous layer | |
| JP2001337065A (ja) | 電気化学メンブレンストリップバイオセンサー | |
| CA2252912A1 (en) | Assay method, kit and assay device | |
| Lu et al. | Rapid, quantitative and sensitive immunochromatographic assay based on stripping voltammetric detection of a metal ion label | |
| WO2004010143A2 (en) | Electrochemical lateral flow and flow-through devices | |
| US20090270266A1 (en) | Method for Electrocatalytic Protein Detection | |
| US12498309B2 (en) | In-situ controlled dissolution of metals using electrochemistry | |
| US20230324274A1 (en) | In-situ controlled dissolution of metals using electrochemistry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070502 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20070502 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20090825 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20091125 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20100126 |
|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20100225 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130305 Year of fee payment: 3 |