JP4279351B2 - 架橋タンパク質結晶処方物、およびその有機溶媒中での触媒としての使用 - Google Patents

架橋タンパク質結晶処方物、およびその有機溶媒中での触媒としての使用 Download PDF

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JP4279351B2
JP4279351B2 JP54264897A JP54264897A JP4279351B2 JP 4279351 B2 JP4279351 B2 JP 4279351B2 JP 54264897 A JP54264897 A JP 54264897A JP 54264897 A JP54264897 A JP 54264897A JP 4279351 B2 JP4279351 B2 JP 4279351B2
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protein
organic solvent
cross
surfactant
crystal
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JP2000514282A (ja
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ネイザー ケイ． カラフ，
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アルタス ファーマシューティカルズ インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP54264897A 1996-05-24 1997-05-20 架橋タンパク質結晶処方物、およびその有機溶媒中での触媒としての使用 Expired - Fee Related JP4279351B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/652,964 1996-05-24
US08/652,964 US5932212A (en) 1996-05-24 1996-05-24 Crosslinked protein crystal formulations and their use as catalysts in organic solvents
PCT/US1997/008526 WO1997044445A1 (en) 1996-05-24 1997-05-20 Cross-linked protein crystal formulations and their use as catalysts in organic solvents

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2007305136A Division JP2008061652A (ja) 1996-05-24 2007-11-26 架橋タンパク質結晶処方物、およびその有機溶媒中での触媒としての使用

Publications (2)

Publication Number Publication Date
JP2000514282A JP2000514282A (ja) 2000-10-31
JP4279351B2 true JP4279351B2 (ja) 2009-06-17

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Family Applications (2)

Application Number Title Priority Date Filing Date
JP54264897A Expired - Fee Related JP4279351B2 (ja) 1996-05-24 1997-05-20 架橋タンパク質結晶処方物、およびその有機溶媒中での触媒としての使用
JP2007305136A Withdrawn JP2008061652A (ja) 1996-05-24 2007-11-26 架橋タンパク質結晶処方物、およびその有機溶媒中での触媒としての使用

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2007305136A Withdrawn JP2008061652A (ja) 1996-05-24 2007-11-26 架橋タンパク質結晶処方物、およびその有機溶媒中での触媒としての使用

Country Status (13)

Country Link
US (2) US5932212A (enEXAMPLES)
EP (1) EP0906417B1 (enEXAMPLES)
JP (2) JP4279351B2 (enEXAMPLES)
KR (1) KR20000015993A (enEXAMPLES)
AR (1) AR007211A1 (enEXAMPLES)
AT (1) ATE393216T1 (enEXAMPLES)
AU (1) AU3072897A (enEXAMPLES)
DE (1) DE69738648T2 (enEXAMPLES)
DK (1) DK0906417T3 (enEXAMPLES)
ID (1) ID17817A (enEXAMPLES)
IN (1) IN191060B (enEXAMPLES)
WO (1) WO1997044445A1 (enEXAMPLES)
ZA (1) ZA974325B (enEXAMPLES)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009750A1 (en) * 1990-11-29 1992-06-11 Willis Richard R Floating contaminant containment systems
US6140475A (en) * 1997-04-11 2000-10-31 Altus Biologics Inc. Controlled dissolution crosslinked protein crystals
JP4433100B2 (ja) * 1997-06-20 2010-03-17 ニューヨーク ユニヴァーシティ チップ及びライブラリの大量製造における物質溶液の静電噴霧
US6833275B1 (en) * 1997-07-22 2004-12-21 Roche Diagnostics Gmbh Gold conjugates containing detergent
DK1009759T3 (da) * 1997-09-05 2008-08-04 Altus Pharmaceuticals Inc Carbohydrat-tværbundne glycoproteinkrystaller
US6500933B1 (en) 1997-09-05 2002-12-31 Altus Biologics Inc. Methods of preparing carbohydrate crosslinked glycoprotein crystals
ZA988461B (en) * 1997-09-18 1999-03-30 Idec Pharma Corp Synergistic composition and methods for treating neoplastic or cancerous growths and for restoring or boosting hematopoiesis
US6541606B2 (en) * 1997-12-31 2003-04-01 Altus Biologics Inc. Stabilized protein crystals formulations containing them and methods of making them
SG121739A1 (en) * 1998-04-27 2006-05-26 Altus Pharmaceuticals Inc Stabilized protein crystals, formulations containing them and methods of making them
IL142393A0 (en) * 1998-10-09 2002-03-10 Altus Biologics Inc Non-homogeneous systems for the resolution of enantiomeric mixtures
US6979561B1 (en) 1998-10-09 2005-12-27 Gilead Sciences, Inc. Non-homogeneous systems for the resolution of enantiomeric mixtures
DE19903125B4 (de) * 1999-01-27 2006-01-05 Sanofi-Aventis Deutschland Gmbh Verfahren zur Trocknung von Kristallen von Insulin oder Insulinanaloga
WO2001012834A1 (en) * 1999-08-18 2001-02-22 Hydrios Biotechnology Oy Process for the preparation and simultaneous separation of enzyme-catalysed products
US20040014195A1 (en) * 1999-12-29 2004-01-22 Diversa Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
US7521216B2 (en) * 1999-12-29 2009-04-21 Verenium Corporation Nitrilases and methods for making and using them
US7608445B1 (en) 1999-12-29 2009-10-27 Verenium Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
US7300775B2 (en) * 1999-12-29 2007-11-27 Verenium Corporation Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents
US20010046493A1 (en) * 2000-02-24 2001-11-29 Alex Margolin Lipase-containing composition and methods of use thereof
WO2001090733A1 (en) * 2000-05-23 2001-11-29 Radiometer Medical A/S A sensor membrane, a method for the preparation thereof, a sensor and a layered membrane structure for such sensor
WO2002072636A2 (en) 2000-12-28 2002-09-19 Altus Biologics Inc. Crystals of whole antibodies and fragments thereof and methods for making and using them
CA2451185A1 (en) 2001-06-21 2003-01-03 Altus Biologics, Inc. Spherical protein particles and methods of making and using them
US7932064B2 (en) 2002-06-13 2011-04-26 Verenium Corporation Processes for making (R)-ethyl 4-cyano-3-hydroxybutyric acid
US9035123B2 (en) 2002-10-01 2015-05-19 The Procter & Gamble Company Absorbent article having a lotioned topsheet
ZA200505306B (en) * 2002-12-31 2006-09-27 Altus Pharmaceuticals Inc Complexes of protein crystals and ionic polymers
SG176314A1 (en) * 2002-12-31 2011-12-29 Altus Pharmaceuticals Inc Human growth hormone crystals and methods for preparing them
US20090035828A1 (en) * 2004-01-01 2009-02-05 Tholath Emilia Abraham Method For the Preparation of Cross Linked Protein Crystals
ES2614157T3 (es) * 2004-10-14 2017-05-29 Eli Lilly And Co. Composiciones que contienen lipasa, proteasa y amilasa para el tratamiento de la insuficiencia pancreática
WO2007050100A2 (en) * 2004-11-24 2007-05-03 Industrial Science & Technology Network, Inc. Immobilized enzymes and processes for preparing and using same
US20060223765A1 (en) * 2005-03-30 2006-10-05 Kimberly-Clark Worldwide, Inc. Method for inhibiting and/or treating vaginal infection
GB0513888D0 (en) * 2005-07-06 2005-08-10 Btg Int Ltd Core 2 GLCNAC-T Inhibitors II
US20100196344A1 (en) * 2005-10-14 2010-08-05 Cystic Fibrosis Foundation Therapeutics, Inc. Compositions and methods for treating pancreatic insufficiency
CA2634053A1 (en) * 2005-12-23 2007-07-05 Altus Pharmaceuticals Inc. Compositions comprising polycation-complexed protein crystals and methods of treatment using them
US20100047413A1 (en) * 2006-09-15 2010-02-25 Cabalka Tourtellotte Leanne M Method of using amphiphilic multiblock copolymers in food
EP2115153B1 (en) 2007-03-01 2013-06-05 BP Corporation North America Inc. Nitrilases, nucleic acids encoding them and methods for making and using them
IL183084A0 (en) * 2007-05-09 2007-09-20 Trans Biodisel Ltd Modified-immobilized enzymes of high tolerance to hydrophilic substrates in organic media
CN101717766B (zh) * 2009-12-01 2012-07-18 暨南大学 蛋白质酶的微水介质固定化方法
AU2011272825A1 (en) 2010-06-30 2013-01-10 Codexis, Inc. Chemically modified carbonic anhydrases useful in carbon capture systems
US9303255B2 (en) 2010-08-26 2016-04-05 Aisin Seiki Kabushiki Kaisha Electrode having enzyme crystals immobilized thereon, method for producing electrode having enzyme crystals immobilized thereon, and biological fuel cell and biosensor provided with electrode having enzyme crystals immobilized thereon

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4267234A (en) * 1978-03-17 1981-05-12 California Institute Of Technology Polyglutaraldehyde synthesis and protein bonding substrates
US4369226A (en) * 1979-03-19 1983-01-18 California Institute Of Technology Polyglutaraldehyde synthesis and protein bonding substrates
US5152903A (en) * 1988-12-19 1992-10-06 American Cyanamid Company Cross-linked cationic polymeric microparticles
ATE240391T1 (de) * 1990-08-03 2003-05-15 Vertex Pharma Verwendung von vernetzten kristallen als neuem verfahren zur immobilisierung von enzymen
GB9309243D0 (en) * 1993-05-05 1993-06-16 Allied Colloids Ltd Enzyme dispersions,their production and compositions containing them
EP0698090B1 (en) * 1993-05-20 1997-11-26 Loders Croklaan B.V. Process for preparing immobilized lipases
ATE193319T1 (de) * 1993-12-03 2000-06-15 Buckman Labor Inc Enzymstabilisierung durch blockcopolymere
DE4344211A1 (de) * 1993-12-23 1995-06-29 Basf Ag Verfahren zur Erhöhung der Aktivität von hydrolytischen Enzymen

Also Published As

Publication number Publication date
ATE393216T1 (de) 2008-05-15
DE69738648T2 (de) 2009-05-28
DK0906417T3 (da) 2008-07-21
US5932212A (en) 1999-08-03
AR007211A1 (es) 1999-10-13
JP2000514282A (ja) 2000-10-31
IN191060B (enEXAMPLES) 2003-09-13
EP0906417A1 (en) 1999-04-07
AU3072897A (en) 1997-12-09
KR20000015993A (ko) 2000-03-25
JP2008061652A (ja) 2008-03-21
US6042824A (en) 2000-03-28
EP0906417B1 (en) 2008-04-23
ID17817A (id) 1998-01-29
WO1997044445A1 (en) 1997-11-27
DE69738648D1 (de) 2008-06-05
ZA974325B (en) 1997-12-18

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