JP4177118B2 - 増幅方法 - Google Patents

増幅方法 Download PDF

Info

Publication number
JP4177118B2
JP4177118B2 JP2002585667A JP2002585667A JP4177118B2 JP 4177118 B2 JP4177118 B2 JP 4177118B2 JP 2002585667 A JP2002585667 A JP 2002585667A JP 2002585667 A JP2002585667 A JP 2002585667A JP 4177118 B2 JP4177118 B2 JP 4177118B2
Authority
JP
Japan
Prior art keywords
ppase
pyrophosphate
reaction mixture
pcr
polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2002585667A
Other languages
English (en)
Japanese (ja)
Other versions
JP2004524853A5 (enrdf_load_stackoverflow
JP2004524853A (ja
Inventor
クラーク，ダンカン・ロイ
ビンセント，スザンヌ・パトリシア
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Defence
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Publication of JP2004524853A publication Critical patent/JP2004524853A/ja
Publication of JP2004524853A5 publication Critical patent/JP2004524853A5/ja
Application granted granted Critical
Publication of JP4177118B2 publication Critical patent/JP4177118B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/525—Phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/101—Hot start

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2002585667A 2001-04-30 2002-04-22 増幅方法 Expired - Fee Related JP4177118B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0110501.4A GB0110501D0 (en) 2001-04-30 2001-04-30 Amplification process
PCT/GB2002/001861 WO2002088387A2 (en) 2001-04-30 2002-04-22 Amplification process

Publications (3)

Publication Number Publication Date
JP2004524853A JP2004524853A (ja) 2004-08-19
JP2004524853A5 JP2004524853A5 (enrdf_load_stackoverflow) 2005-12-22
JP4177118B2 true JP4177118B2 (ja) 2008-11-05

Family

ID=9913697

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2002585667A Expired - Fee Related JP4177118B2 (ja) 2001-04-30 2002-04-22 増幅方法

Country Status (14)

Country Link
US (2) US6951744B2 (enrdf_load_stackoverflow)
EP (1) EP1390532B1 (enrdf_load_stackoverflow)
JP (1) JP4177118B2 (enrdf_load_stackoverflow)
KR (2) KR20100005245A (enrdf_load_stackoverflow)
CN (1) CN1318604C (enrdf_load_stackoverflow)
AR (1) AR035238A1 (enrdf_load_stackoverflow)
AT (1) ATE502118T1 (enrdf_load_stackoverflow)
AU (1) AU2002249444B2 (enrdf_load_stackoverflow)
CA (1) CA2445864C (enrdf_load_stackoverflow)
DE (1) DE60239456D1 (enrdf_load_stackoverflow)
GB (2) GB0110501D0 (enrdf_load_stackoverflow)
NZ (1) NZ528919A (enrdf_load_stackoverflow)
TW (1) TWI323284B (enrdf_load_stackoverflow)
WO (1) WO2002088387A2 (enrdf_load_stackoverflow)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
CN1500887A (zh) * 2002-10-01 2004-06-02 松下电器产业株式会社 引物伸长反应检测方法、碱基种类判别方法及其装置
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
US20060051796A1 (en) * 2004-09-09 2006-03-09 Inga Boell Real time PCR with the addition of pyrophosphatase
GB0502010D0 (en) * 2005-02-01 2005-03-09 Enigma Diagnostics Ltd Biochemical reagents and their uses
ATE502122T1 (de) 2006-06-01 2011-04-15 Trilink Biotechnologies Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
KR101098764B1 (ko) * 2007-10-29 2011-12-26 (주)바이오니아 안정화된 핫스타트 ｐｃｒ용 건조 조성물
CN102105481B (zh) 2008-05-27 2016-02-24 垂林克生物技术公司 用于核酸热启动扩增的化学修饰的核苷5’-三磷酸
JP2013529896A (ja) * 2010-04-30 2013-07-25 エフ．ホフマン−ラ ロシュ アーゲー アクイフェックス・エオリカス由来の無機ピロホスファターゼの精製および使用のためのシステムおよび方法
US20110312708A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Genetic analysis loc for isothermal amplification of nucleic acids
KR101870311B1 (ko) * 2012-03-09 2018-06-25 (주)바이오니아 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물
CN110343673B (zh) 2012-06-14 2024-04-05 生命技术公司 用于聚合酶链式反应(pcr)的新型组合物、方法和试剂盒
GB201301457D0 (en) 2013-01-28 2013-03-13 Fluorogenics Ltd Freeze-dried composition
JP6467829B2 (ja) * 2014-09-03 2019-02-13 東洋紡株式会社 改良されたｒｔ−ｐｃｒ反応
CN104862288B (zh) * 2015-05-08 2019-10-29 苏州大学 一种新型温度响应性无机焦磷酸酶偶联物的合成及其在增强聚合酶链式反应中的应用
EP3402880B1 (en) 2016-01-15 2024-01-03 Thermo Fisher Scientific Baltics UAB Thermophilic dna polymerase mutants
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
JP7234598B2 (ja) * 2018-01-26 2023-03-08 東ソー株式会社 核酸増幅試薬および当該試薬を用いた核酸増幅制御方法
CN113481180A (zh) * 2021-07-05 2021-10-08 吉林大学 碱性嗜热无机焦磷酸酶及其在增强聚合酶链式反应和合成udp-半乳糖反应中的应用
WO2025117716A1 (en) * 2023-11-30 2025-06-05 Watchmaker Genomics, Inc. Taq dna polymerase hotstart antibodies and methods of use thereof

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
CH670709A5 (enrdf_load_stackoverflow) * 1986-04-24 1989-06-30 Univ Moskovsk
US5498523A (en) * 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
JP2997043B2 (ja) * 1989-04-12 2000-01-11 プレジデント アンド フェロウズ オブ ハーバード カレッジ 改良されたプライマー伸長反応
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
FI923911A7 (fi) * 1992-09-01 1994-03-02 Timo Korpela DNA-molekyylien in vitro-synteesi
US5565339A (en) * 1992-10-08 1996-10-15 Hoffmann-La Roche Inc. Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5665551A (en) * 1995-09-13 1997-09-09 Roche Molecular Systems, Inc. Purified nucleic acid encoding a thermostable pyrophosphatase
US6291164B1 (en) * 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
GB9716052D0 (en) 1996-12-06 1997-10-01 Secr Defence Reaction vessels
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
GB9725237D0 (en) 1997-11-29 1998-01-28 Secr Defence Amplification system
GB9803382D0 (en) 1998-02-19 1998-04-15 Secr Defence Detection system
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
KR100292883B1 (ko) * 1999-02-09 2001-06-15 박한오 피로포스페이트와 피로포스파타제를 포함하는 핫 스타트 ｐｃｒ 반응혼합물
CA2395391C (en) 2000-02-23 2011-08-02 City Of Hope Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination
KR20050005626A (ko) * 2003-07-07 2005-01-14 주식회사 포스코 코크스 오븐에서의 장입탄 밀도 향상방법

Also Published As

Publication number Publication date
GB0110501D0 (en) 2001-06-20
CN1522305A (zh) 2004-08-18
KR20040015216A (ko) 2004-02-18
TWI323284B (en) 2010-04-11
AU2002249444B2 (en) 2007-07-12
GB2377991A (en) 2003-01-29
GB2377991B (en) 2004-01-28
DE60239456D1 (de) 2011-04-28
KR101039563B1 (ko) 2011-06-09
ATE502118T1 (de) 2011-04-15
EP1390532B1 (en) 2011-03-16
WO2002088387A2 (en) 2002-11-07
WO2002088387A3 (en) 2003-12-11
US6951744B2 (en) 2005-10-04
GB0209052D0 (en) 2002-05-29
US20030049655A1 (en) 2003-03-13
US20060057617A1 (en) 2006-03-16
EP1390532A2 (en) 2004-02-25
JP2004524853A (ja) 2004-08-19
US7449312B2 (en) 2008-11-11
CA2445864A1 (en) 2002-11-07
AR035238A1 (es) 2004-05-05
CN1318604C (zh) 2007-05-30
KR20100005245A (ko) 2010-01-14
CA2445864C (en) 2011-08-02
NZ528919A (en) 2005-07-29

Similar Documents

Publication Publication Date Title
JP4177118B2 (ja) 増幅方法
US7122355B2 (en) Composition and method for hot start nucleic acid amplification
JP3421664B2 (ja) ヌクレオチド塩基の同定方法
US5487993A (en) Direct cloning of PCR amplified nucleic acids
JP3140937B2 (ja) 好熱酵素を用いる鎖置換増幅法
US10787702B2 (en) Thermolabile exonucleases
EP2824189B1 (en) Composition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction
US20080064071A1 (en) Zwitterionic detergents for the storage and use of DNA polymerases
EP0669401A2 (en) Amplification of long nucleic acid sequences by PCR
AU2002249444A1 (en) Amplification process
US20120219945A1 (en) Use of single-stranded binding protein in amplifying target nucleic acid
US20160130673A1 (en) Nucleic acid detection by oligonucleotide probes cleaved by both exonuclease and endonuclease
CN110446791B (zh) 多核苷酸衔接子及其使用方法
US20020064837A1 (en) Method for synthesizing a nucleic acid molecule using a ribonuclease
EP1548112B1 (en) Method of amplifying nucleic acid, reagent kit for amplifying nucleic acid, method of detecting single nucleotide polymorphism and reagent kit for detecting single nucleotide polymorphism
JP2007319096A (ja) エンドヌクレアーゼのニッキング活性を利用した核酸増幅法
EP1277841B1 (en) New composition and method for hot start nucleic acid amplification
JPH0851979A (ja) Ｒｎａの逆転写方法およびその用途
WO2025032340A1 (en) Method of amplifying methylated dna
KR20170004823A (ko) 엑소뉴클레아제 활성이 결여된 폴리머라제를 이용한 fret 기반의 핵산검출 방법
WO2002101041A1 (fr) Procede d'amplification de l'acide nucleique et procede de detection du polymorphisme des nucleotides a l'aide d'un analogue de nucleotide

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20040630

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20041118

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20070814

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20071107

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20071114

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20080214

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20080325

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20080620

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20080729

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20080821

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110829

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110829

Year of fee payment: 3

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120829

Year of fee payment: 4

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120829

Year of fee payment: 4

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130829

Year of fee payment: 5

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees