JP4176890B2 - カルボキシドテルムス・ヒドロゲノフォルマンス由来の修飾dnaポリメラーゼ並びに連結させた逆転写−ポリメラーゼ連鎖反応におけるその使用 - Google Patents

カルボキシドテルムス・ヒドロゲノフォルマンス由来の修飾dnaポリメラーゼ並びに連結させた逆転写−ポリメラーゼ連鎖反応におけるその使用 Download PDF

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JP4176890B2
JP4176890B2 JP34348798A JP34348798A JP4176890B2 JP 4176890 B2 JP4176890 B2 JP 4176890B2 JP 34348798 A JP34348798 A JP 34348798A JP 34348798 A JP34348798 A JP 34348798A JP 4176890 B2 JP4176890 B2 JP 4176890B2
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dna
polymerase
dna polymerase
reverse transcription
rna
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JPH11225786A (ja
JPH11225786A5 (enExample
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アンケンバウワー ワルトラウド
マルカウ ウルスラ
エベンビヒラー クリスティン
アッハマー ガンサー
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ロシュ ダイアグノスティックス ゲーエムベーハー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP34348798A 1997-12-02 1998-12-02 カルボキシドテルムス・ヒドロゲノフォルマンス由来の修飾dnaポリメラーゼ並びに連結させた逆転写−ポリメラーゼ連鎖反応におけるその使用 Expired - Lifetime JP4176890B2 (ja)

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DE97121151:1 1997-12-02
EP97121151A EP0921196A1 (en) 1997-12-02 1997-12-02 Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
US09/204,208 US6399320B1 (en) 1997-12-02 1998-12-01 Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction

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JPH11225786A JPH11225786A (ja) 1999-08-24
JPH11225786A5 JPH11225786A5 (enExample) 2005-12-02
JP4176890B2 true JP4176890B2 (ja) 2008-11-05

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JP (1) JP4176890B2 (enExample)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3910015B2 (ja) 1998-11-27 2007-04-25 タカラバイオ株式会社 cDNAの合成方法
DE19920611A1 (de) * 1999-05-05 2000-11-09 Roche Diagnostics Gmbh Verfahren zur 5'-Cap-abhängigen Anreicherung von cDNAs
US20040009486A1 (en) * 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US20050123940A1 (en) * 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
EP1532275A4 (en) * 2002-07-26 2005-09-14 Applera Corp HOT START-UP BIOCHEMICAL REACTIONS BY MG
US7662594B2 (en) * 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
US7820030B2 (en) * 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
EP1740711A2 (en) 2004-04-01 2007-01-10 Bio-Rad Laboratories, Inc. Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity
US7455844B2 (en) 2006-03-29 2008-11-25 Merial Limited Vaccine against streptococci
DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
CN101495656B (zh) * 2006-06-07 2017-02-08 纽约哥伦比亚大学理事会 采用带修饰的核苷酸通过纳米通道进行dna序列测定
US20080076123A1 (en) * 2006-09-27 2008-03-27 Helicos Biosciences Corporation Polymerase variants for DNA sequencing
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
US8530164B2 (en) * 2008-09-05 2013-09-10 Pacific Biosciences Of California, Inc. Method for sequencing using branching fraction of incorporatable nucleotides
EP2271751B1 (en) * 2008-03-31 2015-07-22 Pacific Biosciences of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8133672B2 (en) 2008-03-31 2012-03-13 Pacific Biosciences Of California, Inc. Two slow-step polymerase enzyme systems and methods
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
US8420366B2 (en) * 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8685648B2 (en) 2009-02-03 2014-04-01 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
US20120021460A1 (en) * 2010-01-08 2012-01-26 Qiagen Gaithersburg Inc. Materials and methods for isothermal nucleic acid amplification
US20110192723A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
US9605307B2 (en) * 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
CN103003418B (zh) 2010-06-18 2015-08-26 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
ES2536252T3 (es) 2010-06-18 2015-05-21 F. Hoffmann-La Roche Ag ADN polimerasas con diferenciación de desapareamientos 3' aumentada
WO2011157434A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CN103025869B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
EP2582806B1 (en) 2010-06-18 2015-12-16 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
EP2582803B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
ES2564692T3 (es) 2010-06-18 2016-03-28 F. Hoffmann-La Roche Ag ADN polimerasas con discriminación incrementada de no correspondencia 3'
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
WO2012009206A2 (en) 2010-07-12 2012-01-19 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
EP2675897B1 (en) 2011-02-15 2016-05-11 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
CA2839964C (en) 2011-07-28 2019-03-12 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
EP2788480B1 (en) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna polymerases with improved activity
WO2013083263A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 改善された活性を有するdnaポリメラーゼ
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
GB2559073A (en) 2012-06-08 2018-07-25 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
JP2015525077A (ja) 2012-06-15 2015-09-03 ジェニア・テクノロジーズ・インコーポレイテッド チップの構成および高精度な核酸配列決定
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
EP3060918B1 (en) 2013-10-23 2019-09-18 Genia Technologies, Inc. High speed molecular sensing with nanopores
EP3709003A1 (en) 2013-11-17 2020-09-16 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
US9885657B2 (en) 2014-08-08 2018-02-06 Quantum-Si Incorporated Integrated device with external light source for probing detecting and analyzing molecules
US12655467B2 (en) 2014-08-08 2026-06-16 Quantum-Si Incorporated Methods for nucleic acid sequencing
CN111710687B (zh) 2014-08-08 2023-11-14 宽腾矽公司 基于入射光子到达时间的识别、成像、测序法及存储介质
KR102738114B1 (ko) 2014-08-08 2024-12-06 퀀텀-에스아이 인코포레이티드 분자들을 프로빙, 검출 및 분석하기 위한 광학계 및 검정 칩
TW202139919A (zh) 2016-02-17 2021-11-01 美商太斯萊特健康股份有限公司 用於生命期成像及偵測應用之感測器及裝置
CN110168732B (zh) 2016-12-22 2024-03-08 宽腾矽公司 具有直接合并像素的整合式光电侦测器
CA3069983A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated Hand-held, massively-parallel, bio-optoelectronic instrument
ES2969883T3 (es) 2018-03-21 2024-05-23 Hoffmann La Roche ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados
MX2020013788A (es) 2018-06-22 2021-03-02 Quantum Si Inc Fotodetector integrado con deposito de almacenamiento de carga con tiempo de deteccion variado.
JP7504872B2 (ja) 2018-09-13 2024-06-24 エフ. ホフマン-ラ ロシュ アーゲー 改善されたストランド置換能力を有する変異体dnaポリメラーゼ
JP2024008527A (ja) * 2022-07-08 2024-01-19 東洋紡株式会社 マンガンを使用しない逆転写方法
WO2026153862A1 (en) 2025-01-14 2026-07-23 F. Hoffmann-La Roche Ag Dna polymerases with improved activity

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5693517A (en) 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
JP2968585B2 (ja) * 1989-12-22 1999-10-25 エフ.ホフマン ― ラ ロシュ アーゲー 高温度逆転写酵素
JP2709311B2 (ja) 1990-09-28 1998-02-04 エフ.ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性dnaポリメラーゼの5→3′のエキソヌクレアーゼ突然変異
WO1992006188A2 (en) 1990-10-05 1992-04-16 Barnes Wayne M Thermostable dna polymerase
EP0517418B1 (en) 1991-06-03 2003-11-26 Takara Bio Inc. A method for cloning of a gene for Pol I type DNA polymerase
US5474920A (en) 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
US6100078A (en) 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
AU4147796A (en) 1994-11-04 1996-05-31 Molecular Biology Resources, Inc. Dna encoding a thermostable dna polymerase enzyme
US5834253A (en) 1994-11-17 1998-11-10 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity
US5814506A (en) 1995-08-02 1998-09-29 New England Biolabs, Inc. Over-expression and purification of a truncated thermostable DNA polymerase by protein fusion
JP4073038B2 (ja) 1995-12-15 2008-04-09 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション テルモアナエロバクター・テルモヒドロスルフリカスからの熱安定性dnaポリメラーゼおよびエキソヌクレアーゼ活性が除去されているその変異体酵素
US5830714A (en) 1996-04-17 1998-11-03 Molecular Biology Resources, Inc. Biologically active fragment of bacillus stearothermophilus DNA polymerase
EP0834569A1 (en) * 1996-10-03 1998-04-08 Roche Diagnostics GmbH Thermostable DNA polymerase from carboxydothermus hydrogenoformans

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Publication number Publication date
JPH11225786A (ja) 1999-08-24
US20020160399A1 (en) 2002-10-31
US6794177B2 (en) 2004-09-21
US6399320B1 (en) 2002-06-04
EP0921196A1 (en) 1999-06-09

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