JP4176890B2 - カルボキシドテルムス・ヒドロゲノフォルマンス由来の修飾dnaポリメラーゼ並びに連結させた逆転写−ポリメラーゼ連鎖反応におけるその使用 - Google Patents
カルボキシドテルムス・ヒドロゲノフォルマンス由来の修飾dnaポリメラーゼ並びに連結させた逆転写−ポリメラーゼ連鎖反応におけるその使用 Download PDFInfo
- Publication number
- JP4176890B2 JP4176890B2 JP34348798A JP34348798A JP4176890B2 JP 4176890 B2 JP4176890 B2 JP 4176890B2 JP 34348798 A JP34348798 A JP 34348798A JP 34348798 A JP34348798 A JP 34348798A JP 4176890 B2 JP4176890 B2 JP 4176890B2
- Authority
- JP
- Japan
- Prior art keywords
- dna
- polymerase
- dna polymerase
- reverse transcription
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE97121151:1 | 1997-12-02 | ||
| EP97121151A EP0921196A1 (en) | 1997-12-02 | 1997-12-02 | Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction |
| US09/204,208 US6399320B1 (en) | 1997-12-02 | 1998-12-01 | Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPH11225786A JPH11225786A (ja) | 1999-08-24 |
| JPH11225786A5 JPH11225786A5 (enExample) | 2005-12-02 |
| JP4176890B2 true JP4176890B2 (ja) | 2008-11-05 |
Family
ID=26145941
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP34348798A Expired - Lifetime JP4176890B2 (ja) | 1997-12-02 | 1998-12-02 | カルボキシドテルムス・ヒドロゲノフォルマンス由来の修飾dnaポリメラーゼ並びに連結させた逆転写−ポリメラーゼ連鎖反応におけるその使用 |
Country Status (3)
| Country | Link |
|---|---|
| US (2) | US6399320B1 (enExample) |
| EP (1) | EP0921196A1 (enExample) |
| JP (1) | JP4176890B2 (enExample) |
Families Citing this family (68)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3910015B2 (ja) | 1998-11-27 | 2007-04-25 | タカラバイオ株式会社 | cDNAの合成方法 |
| DE19920611A1 (de) * | 1999-05-05 | 2000-11-09 | Roche Diagnostics Gmbh | Verfahren zur 5'-Cap-abhängigen Anreicherung von cDNAs |
| US20040009486A1 (en) * | 1999-10-29 | 2004-01-15 | Sorge Joseph A. | Compositions and methods utilizing DNA polymerases |
| US20050123940A1 (en) * | 1999-10-29 | 2005-06-09 | Stratagene California | Compositions and methods for synthesizing cDNA |
| US20030228616A1 (en) * | 1999-10-29 | 2003-12-11 | Stratagene | DNA polymerase mutants with reverse transcriptase activity |
| EP1532275A4 (en) * | 2002-07-26 | 2005-09-14 | Applera Corp | HOT START-UP BIOCHEMICAL REACTIONS BY MG |
| US7662594B2 (en) * | 2002-09-20 | 2010-02-16 | New England Biolabs, Inc. | Helicase-dependent amplification of RNA |
| US7820030B2 (en) * | 2003-04-16 | 2010-10-26 | Handylab, Inc. | System and method for electrochemical detection of biological compounds |
| EP1740711A2 (en) | 2004-04-01 | 2007-01-10 | Bio-Rad Laboratories, Inc. | Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity |
| US7455844B2 (en) | 2006-03-29 | 2008-11-25 | Merial Limited | Vaccine against streptococci |
| DE102006025154A1 (de) * | 2006-05-30 | 2007-12-06 | Universität Konstanz | Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität |
| CN101495656B (zh) * | 2006-06-07 | 2017-02-08 | 纽约哥伦比亚大学理事会 | 采用带修饰的核苷酸通过纳米通道进行dna序列测定 |
| US20080076123A1 (en) * | 2006-09-27 | 2008-03-27 | Helicos Biosciences Corporation | Polymerase variants for DNA sequencing |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8652781B2 (en) | 2008-02-12 | 2014-02-18 | Pacific Biosciences Of California, Inc. | Cognate sampling kinetics |
| US8530164B2 (en) * | 2008-09-05 | 2013-09-10 | Pacific Biosciences Of California, Inc. | Method for sequencing using branching fraction of incorporatable nucleotides |
| EP2271751B1 (en) * | 2008-03-31 | 2015-07-22 | Pacific Biosciences of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
| US8133672B2 (en) | 2008-03-31 | 2012-03-13 | Pacific Biosciences Of California, Inc. | Two slow-step polymerase enzyme systems and methods |
| US8999676B2 (en) | 2008-03-31 | 2015-04-07 | Pacific Biosciences Of California, Inc. | Recombinant polymerases for improved single molecule sequencing |
| US8420366B2 (en) * | 2008-03-31 | 2013-04-16 | Pacific Biosciences Of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
| US8685648B2 (en) | 2009-02-03 | 2014-04-01 | Biohelix Corporation | Endonuclease-enhanced helicase-dependent amplification |
| US20120021460A1 (en) * | 2010-01-08 | 2012-01-26 | Qiagen Gaithersburg Inc. | Materials and methods for isothermal nucleic acid amplification |
| US20110192723A1 (en) * | 2010-02-08 | 2011-08-11 | Genia Technologies, Inc. | Systems and methods for manipulating a molecule in a nanopore |
| US9605307B2 (en) * | 2010-02-08 | 2017-03-28 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
| US9678055B2 (en) | 2010-02-08 | 2017-06-13 | Genia Technologies, Inc. | Methods for forming a nanopore in a lipid bilayer |
| US8324914B2 (en) | 2010-02-08 | 2012-12-04 | Genia Technologies, Inc. | Systems and methods for characterizing a molecule |
| CN103003418B (zh) | 2010-06-18 | 2015-08-26 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
| ES2536252T3 (es) | 2010-06-18 | 2015-05-21 | F. Hoffmann-La Roche Ag | ADN polimerasas con diferenciación de desapareamientos 3' aumentada |
| WO2011157434A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| CN103025869B (zh) | 2010-06-18 | 2015-07-01 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
| EP2582806B1 (en) | 2010-06-18 | 2015-12-16 | Roche Diagnostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| EP2582803B1 (en) | 2010-06-18 | 2015-02-25 | Roche Diagnostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| ES2564692T3 (es) | 2010-06-18 | 2016-03-28 | F. Hoffmann-La Roche Ag | ADN polimerasas con discriminación incrementada de no correspondencia 3' |
| WO2011157436A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| WO2012009206A2 (en) | 2010-07-12 | 2012-01-19 | Pacific Biosciences Of California, Inc. | Sequencing reactions with alkali metal cations for pulse width control |
| US9121059B2 (en) | 2010-12-22 | 2015-09-01 | Genia Technologies, Inc. | Nanopore-based single molecule characterization |
| US8962242B2 (en) | 2011-01-24 | 2015-02-24 | Genia Technologies, Inc. | System for detecting electrical properties of a molecular complex |
| US9110478B2 (en) | 2011-01-27 | 2015-08-18 | Genia Technologies, Inc. | Temperature regulation of measurement arrays |
| EP2675897B1 (en) | 2011-02-15 | 2016-05-11 | Roche Diagnostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| ES2561885T3 (es) | 2011-04-11 | 2016-03-01 | F. Hoffmann-La Roche Ag | ADN polimerasas de actividad mejorada |
| CA2839964C (en) | 2011-07-28 | 2019-03-12 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
| EP2788480B1 (en) | 2011-12-08 | 2019-01-16 | Roche Diagnostics GmbH | Dna polymerases with improved activity |
| WO2013083263A1 (en) | 2011-12-08 | 2013-06-13 | Roche Diagnostics Gmbh | Dna polymerases with improved activity |
| JP6144697B2 (ja) | 2011-12-08 | 2017-06-07 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 改善された活性を有するdnaポリメラーゼ |
| US8986629B2 (en) | 2012-02-27 | 2015-03-24 | Genia Technologies, Inc. | Sensor circuit for controlling, detecting, and measuring a molecular complex |
| GB2559073A (en) | 2012-06-08 | 2018-07-25 | Pacific Biosciences California Inc | Modified base detection with nanopore sequencing |
| JP2015525077A (ja) | 2012-06-15 | 2015-09-03 | ジェニア・テクノロジーズ・インコーポレイテッド | チップの構成および高精度な核酸配列決定 |
| CA2877094A1 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US9399766B2 (en) | 2012-10-01 | 2016-07-26 | Pacific Biosciences Of California, Inc. | Recombinant polymerases for incorporation of protein shield nucleotide analogs |
| US9605309B2 (en) | 2012-11-09 | 2017-03-28 | Genia Technologies, Inc. | Nucleic acid sequencing using tags |
| US9759711B2 (en) | 2013-02-05 | 2017-09-12 | Genia Technologies, Inc. | Nanopore arrays |
| US9551697B2 (en) | 2013-10-17 | 2017-01-24 | Genia Technologies, Inc. | Non-faradaic, capacitively coupled measurement in a nanopore cell array |
| US9567630B2 (en) | 2013-10-23 | 2017-02-14 | Genia Technologies, Inc. | Methods for forming lipid bilayers on biochips |
| EP3060918B1 (en) | 2013-10-23 | 2019-09-18 | Genia Technologies, Inc. | High speed molecular sensing with nanopores |
| EP3709003A1 (en) | 2013-11-17 | 2020-09-16 | Quantum-Si Incorporated | Optical system and assay chip for probing, detecting and analyzing molecules |
| US10174363B2 (en) | 2015-05-20 | 2019-01-08 | Quantum-Si Incorporated | Methods for nucleic acid sequencing |
| US9885657B2 (en) | 2014-08-08 | 2018-02-06 | Quantum-Si Incorporated | Integrated device with external light source for probing detecting and analyzing molecules |
| US12655467B2 (en) | 2014-08-08 | 2026-06-16 | Quantum-Si Incorporated | Methods for nucleic acid sequencing |
| CN111710687B (zh) | 2014-08-08 | 2023-11-14 | 宽腾矽公司 | 基于入射光子到达时间的识别、成像、测序法及存储介质 |
| KR102738114B1 (ko) | 2014-08-08 | 2024-12-06 | 퀀텀-에스아이 인코포레이티드 | 분자들을 프로빙, 검출 및 분석하기 위한 광학계 및 검정 칩 |
| TW202139919A (zh) | 2016-02-17 | 2021-11-01 | 美商太斯萊特健康股份有限公司 | 用於生命期成像及偵測應用之感測器及裝置 |
| CN110168732B (zh) | 2016-12-22 | 2024-03-08 | 宽腾矽公司 | 具有直接合并像素的整合式光电侦测器 |
| CA3069983A1 (en) | 2017-07-24 | 2019-01-31 | Quantum-Si Incorporated | Hand-held, massively-parallel, bio-optoelectronic instrument |
| ES2969883T3 (es) | 2018-03-21 | 2024-05-23 | Hoffmann La Roche | ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados |
| MX2020013788A (es) | 2018-06-22 | 2021-03-02 | Quantum Si Inc | Fotodetector integrado con deposito de almacenamiento de carga con tiempo de deteccion variado. |
| JP7504872B2 (ja) | 2018-09-13 | 2024-06-24 | エフ. ホフマン-ラ ロシュ アーゲー | 改善されたストランド置換能力を有する変異体dnaポリメラーゼ |
| JP2024008527A (ja) * | 2022-07-08 | 2024-01-19 | 東洋紡株式会社 | マンガンを使用しない逆転写方法 |
| WO2026153862A1 (en) | 2025-01-14 | 2026-07-23 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| CA1338457C (en) | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
| US5693517A (en) | 1987-06-17 | 1997-12-02 | Roche Molecular Systems, Inc. | Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions |
| US5374553A (en) * | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| JP2968585B2 (ja) * | 1989-12-22 | 1999-10-25 | エフ.ホフマン ― ラ ロシュ アーゲー | 高温度逆転写酵素 |
| JP2709311B2 (ja) | 1990-09-28 | 1998-02-04 | エフ.ホフマン−ラ ロシュ アクチェンゲゼルシャフト | 熱安定性dnaポリメラーゼの5→3′のエキソヌクレアーゼ突然変異 |
| WO1992006188A2 (en) | 1990-10-05 | 1992-04-16 | Barnes Wayne M | Thermostable dna polymerase |
| EP0517418B1 (en) | 1991-06-03 | 2003-11-26 | Takara Bio Inc. | A method for cloning of a gene for Pol I type DNA polymerase |
| US5474920A (en) | 1993-11-23 | 1995-12-12 | State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University | Modified thermo-resistant DNA polymerases |
| US6100078A (en) | 1994-04-01 | 2000-08-08 | Gen-Probe Incorporated | Purified DNA polymerase from bacillus stearothermophilus ATCC 12980 |
| US5912155A (en) * | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
| AU4147796A (en) | 1994-11-04 | 1996-05-31 | Molecular Biology Resources, Inc. | Dna encoding a thermostable dna polymerase enzyme |
| US5834253A (en) | 1994-11-17 | 1998-11-10 | Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences | Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity |
| US5814506A (en) | 1995-08-02 | 1998-09-29 | New England Biolabs, Inc. | Over-expression and purification of a truncated thermostable DNA polymerase by protein fusion |
| JP4073038B2 (ja) | 1995-12-15 | 2008-04-09 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | テルモアナエロバクター・テルモヒドロスルフリカスからの熱安定性dnaポリメラーゼおよびエキソヌクレアーゼ活性が除去されているその変異体酵素 |
| US5830714A (en) | 1996-04-17 | 1998-11-03 | Molecular Biology Resources, Inc. | Biologically active fragment of bacillus stearothermophilus DNA polymerase |
| EP0834569A1 (en) * | 1996-10-03 | 1998-04-08 | Roche Diagnostics GmbH | Thermostable DNA polymerase from carboxydothermus hydrogenoformans |
-
1997
- 1997-12-02 EP EP97121151A patent/EP0921196A1/en not_active Withdrawn
-
1998
- 1998-12-01 US US09/204,208 patent/US6399320B1/en not_active Expired - Lifetime
- 1998-12-02 JP JP34348798A patent/JP4176890B2/ja not_active Expired - Lifetime
-
2002
- 2002-02-05 US US10/072,436 patent/US6794177B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH11225786A (ja) | 1999-08-24 |
| US20020160399A1 (en) | 2002-10-31 |
| US6794177B2 (en) | 2004-09-21 |
| US6399320B1 (en) | 2002-06-04 |
| EP0921196A1 (en) | 1999-06-09 |
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