JP4033353B2 - Non-pesticide shiitake mushrooms, shimeji mushrooms, mushroom reishi mushroom seeding method and shiitake mushroom mushrooms mushroom mushroom mushrooms - Google Patents

Description

The present invention, shiitake by Organic with skewer or toothpick, shimeji, Ganoderma lucidum, and antler Reishi mushroom seeding method, shiitake, shimeji, Ganoderma lucidum, to a method of cultivating Kazuno Reishi.

Conventionally, using this kind of toothpick with no pesticides, such as shiitake mushrooms, shimeji mushrooms, bamboo shoots, etc. JP-A-2002-84884 proposed by the present applicant. The content of the present invention is basically an operation in which a large number of toothpicks are put in a sterilized and air-permeable bag and sealed, and a fruiting body piece is inserted into the sealed bag to seed the koji mold. `` Method of sowing seeds such as shiitake mushrooms, shimeji mushrooms, garlic mushrooms, etc. that produce the original fungus '' and `` Put the log with seeds in a breathable bag, boil sterilize the log with the bag, seal the bag, Provide an inclusion such as a sponge, insert the toothpick with the inoculum into the bag through the inclusion, install it in the seeding part of the sterilized raw wood, lay down the raw wood with the toothpick and then embed it in the ground Is a cultivation method of a cocoon that makes a real concealment. "
JP-A-2002-84884

  The document (1) is efficient and has a great economic effect because it can produce the original bacteria if it is obtained for a very short period of time, approximately 2 weeks. Moreover, the production | generation of this original microbe can be performed simply and reliably. It is clear that this document (1) having these characteristics brings a secondary sound to the industry.

  However, since the document (1) does not clearly disclose the treatment for the presence or absence of pesticides, it is not necessarily sufficient for the demand for pesticide-free cultivation or pesticide-free methods that have been attracting attention recently. It is done.

Therefore, the present invention has the characteristics of this document (1), and further improvements can be applied to the recent almost completely pesticide-free cultivation, or pesticide-free shiitake mushrooms, shimeji mushrooms, garlic mushrooms, deer horn spirits. The present invention provides a method of sowing seeds of turf and a method of cultivating raw cocoons and deer horned turf that can be applied to almost completely pesticide-free cultivation or pesticide-free cultivation.

The invention of claim 1 provides a method for sowing seeds of shiitake mushrooms, shimeji mushrooms, scallops, deer horned turf by means of pesticide-free cultivation that can be applied to substantially pesticide-free cultivation, or pesticide-free cultivation, while shortening the production of mycelia, It is intended to meet the needs of the industry or Ichiai with a simple and reliable seeding. In addition, the present invention provides a pesticide-free shiitake mushroom, shimeji mushroom, sardine reishi mushroom that can respond to recent demands through the provision of this seeding method, or a pesticide-free shiitake mushroom, shimeji mushroom, mannentake, Intended to provide Kakuno Reishi cocoons .

[Claim 1] The fruit body piece and several toothpicks are put in a bag having air permeability and sealed, and the air bag having this fruit body piece and several toothpicks is treated in hot water. After a few days, the hyphae were seeded with this hyphae, and the hyphae with hyphae were stabbed into another bag containing a large number of toothpicks with antimicrobial invasion prevention means such as antimicrobial invasion tape and sponge, A pesticide-free shiitake mushroom, shimeji mushroom, which is configured to generate hyphae on a plurality of toothpicks in this other bag while performing this stab from the above-mentioned other bacteria invasion prevention means to prevent the invasion of other bacteria into the other bag. This is a method for seeding sardine, sardine and reindeer .

  The invention of claim 2 is intended to achieve the object of claim 1 and prevent invasion of germs into the bag and culture in a sterile state (generation of mycelia).

Claim 2 is a pesticide-free shiitake mushroom, shimeji mushroom, garlic mushroom, deer horned spirit, which is configured to rinse the hyphae with mycelia with purified water for about 1 second when pierced through the invasion prevention means according to claim 1 It is a method of sowing seeds .

  The invention of claim 3 is intended to achieve the object of claim 1 and to ensure the sterility of the bag containing the fruit body pieces and several toothpicks.

Claim 3 is a pesticide-free shiitake mushroom having a treatment time of about 10 seconds to 15 seconds when treating the bag containing the fruit body pieces and several toothpicks according to claim 1 in hot water , This is a method of sowing seeds of shimeji mushrooms, mannentake, and kakukaku reishi .

A fourth aspect of the present invention, substantially completely organic cultivation, or shiitake by Organic accommodate pesticide-free, shimeji, Ganoderma lucidum, using mushrooms seeding method antler Reishi, Shiitake by Organic to respond to recent demands It is intended to provide shimeji mushrooms, shimeji mushrooms, kakukaku reishi mushrooms, or shiitake mushrooms, shimeji mushrooms, kakukaku reishi mushrooms with no pesticides useful for health. In addition, the present invention is intended to shorten the cultivation method, to simplify and generalize, or to appropriately change the number of needles with mycelia stabbed into the bag so as to accelerate the spread period of the fungus, etc. To do.

Claim 4 collects shiitake mushrooms, shimeji mushrooms, sardine mushrooms, deer horned turf that grow naturally in Yamano, and removes the fruit body stains and various foreign substances with paper etc., and makes a part of the fruit body of this pure fungus to an appropriate size. Cut and make fruit body pieces, which contain the fruit body pieces together with one or several sterilized toothpicks with the nutrients of rice bran contained in a breathable bag. Attaching to the body piece, the air in the bag is extracted, and the end of the extracted bag is tied with a rubber band, a string, or a tightening means of squeezing. After the sterilization is completed, the bag near the toothpick with the toothpick is twisted and sealed, and the bag containing the sterilized fruiting with the toothpick is placed in the dark. Cultivate in a natural temperature environment, and use None,
In order to produce a large number of the same kind of toothpick inoculum for this original fungus, first, put a lot of toothpick and rice bran in a pot filled with water, boil the pot, and in this toothpick When the rice bran nutrient has permeated, after taking out and drying, this toothpick is housed in a bag, and the air in the bag is evacuated and sealed, preventing invasion of germs into the bag, and disinfecting with hot water. Since this sterilized toothpick becomes a culture medium, a bag containing this culture medium is provided with antimicrobial invasion prevention means such as tape and sponge, and the antimicrobial invasion prevention means is inserted into the antimicrobial invasion prevention means, Can be produced by cultivating the above-mentioned toothpick inoculum into the raw wood, and after producing the cultured raw wood, this cultured raw wood is stacked and suspended, and buried in the ground or in a flower pot. shiitake by pesticide-free, which is configured to be this face down in Mushroom, Ganoderma lucidum, is a cultivation method of Kazuno Ganoderma lucidum.

In the invention of claim 1, the fruit body piece and several toothpicks are put in a bag having air permeability and sealed, and the breathable bag containing the fruit body pieces and several toothpicks is put in hot water. After treatment, several days later, the hypha is seeded on the toothpick, and the toothpick with the hypha is stabbed into another bag containing a large number of toothpicks equipped with means for preventing invasion of various germs such as a tape and sponge for invasion of various germs. This is a pesticide-free shiitake mushroom, shimeji mushroom, garlic mushroom , deer horned turf mushroom that produces hyphae on a number of toothpicks in another bag while preventing the invasion of other bacteria into the other bag. It is a seeding method.

Therefore, claim 1 provides a method for sowing seeds of shiitake mushrooms, shimeji mushrooms, mannentake, kakukaku reishi, which are compatible with almost complete pesticide-free cultivation, or pesticide-free cultivation, while shortening the production of mycelia, There is a feature that can meet the demands of the industry or Ichiai by simple and reliable seeding. In addition, the present invention provides a pesticide-free shiitake mushroom, shimeji mushroom, sardine reishi mushroom that can respond to recent demands through the provision of this seeding method, or a pesticide-free shiitake mushroom, shimeji mushroom, mannentake, There is a feature that can provide the Kakuno Reishi cocoon .

The invention of claim 2 is a pesticide-free shiitake mushroom, shimeji mushroom, garlic mushroom, deer horn which is configured to rinse the toothpick with hyphae for about 1 second with purified water when piercing through the means for preventing invasion of various bacteria according to claim 1. This is a method for seeding Ganoderma .

  Therefore, claim 2 is characterized in that the object of claim 1 can be achieved, and that germs can be prevented from entering the bag and culturing in a sterile state (mycelium production) can be achieved.

The invention according to claim 3 is a pesticide-free shiitake mushroom in which the treatment time when the bag containing the fruit body piece and several toothpicks according to claim 1 is treated in hot water is about 10 to 15 seconds. , Shimeji, mannentake and kakunoreshi .

  Accordingly, the third aspect is characterized in that the object of the first aspect can be achieved, and the aseptic condition of the bag containing the fruit body pieces and several toothpicks can be secured.

The invention of claim 4 collects shiitake mushrooms, shimeji mushrooms, sardine mushrooms, deer horned turf that grow naturally in Yamano, and removes the fruit body stains and various foreign substances with paper, etc. Cut into two pieces to make a fruiting piece, containing the fruiting piece together with one or several sterilized toothpicks provided with nutrients of rice bran contained in a breathable bag, Attach to the piece, draw out air from the bag, bind the end of the removed bag with rubber band, string, tightening tightening means, and immerse the bag containing the fruiting piece with toothpick in hot water When the sterilization is completed, the bag in the vicinity of the fruiting piece with the toothpick is twisted and sealed, and then the bag containing the sterilized fruiting piece with the toothpick is placed in a dark place in a natural temperature environment. Cultivate to produce a spp.
In order to produce a large number of the same type of toothpick inoculum for the original fungus, first, put a lot of toothpick and rice bran in a pot filled with water, boil the pot, When the nutrients permeate, take out and dry, and then place the toothpick in a bag and remove the air in the bag and seal it to prevent the invasion of germs in the bag. Since the medium becomes a medium, the bacteria containing invasion prevention tape / sponge is provided in the bag containing the medium, and the infestation means for the original bacteria is inserted into the invasion preventing means and cultured in a dark room. Since seedlings can be produced, pesticide seedlings are stabbed into the raw wood to produce cultured raw wood, and then the cultured raw wood is stacked and temporarily suspended, embedded in the ground or in a flower pot, and configured to lie down shiitake mushrooms, mushroom, Ganoderma lucidum, in Kazuno Ganoderma cultivation method That.

Accordingly, claim 4 is substantially completely organic cultivation, or shiitake by Organic accommodate pesticide-free, shimeji, Ganoderma lucidum, using mushrooms seeding method antler Reishi, Shiitake by Organic to respond to recent demands , Shimeji mushroom, mushroom reishi mushrooms, and mushroom reishi mushrooms, shimeji mushrooms, mushroom mushrooms, kokukaku reishi mushrooms, which are useful for health. Further, the present invention has an advantage that the cultivation method can be shortened, simplified and generalized. In addition, the present invention is characterized in that the spread period of the fungus can be shortened by appropriately changing the number of the toothpick with the mycelia inserted into the bag.

An example of the present invention will be described.
FIGS. 1 to 17 show a method of sowing seeds such as shiitake mushrooms, shimeji mushrooms, sardine mushrooms, etc. by an agrochemical that produces hyphae on a large number of toothpicks in a bag, and a preferred example of this method will be described. Collect Ganoderma mushrooms that grow naturally in Yamano (Figure 1). This mannamboe is a fresh and undamaged fruit body 1, and dirt, various foreign matters, etc. of this fruit body 1 are removed with paper or the like, and in principle, it is not washed with water. This is because there is a risk of inhaling various bacteria by this washing, and usually the fruiting body 1 is pure bacteria, and this dirt and various foreign substances are attached to the surface. Is enough and there is no problem. A part of the fruit body 1 is cut into an appropriate size to form a fruit body piece 2 (FIG. 2). And what is prepared in another is the bag 3 with air permeability, and the sterilized skewer or toothpick 4 (commercially available toothpick is simple) to which the nutrients of the rice bran contained in this bag are given ( FIG. 3). Then, one or several toothpicks 4 taken out from the bag 3 are brought into contact with the fruit body piece 2 and secured with a fastening tool 5 such as a rubber band or a string (FIG. 4). The fruiting body piece 2 with the toothpick 4 is accommodated in a breathable bag 3 (thickness of about 0.01 mm is ideal), and then the air in the bag 3 is extracted and the end of the extracted bag 3 is It is bound with a tightening tool 5 such as a rubber band, a string, or a tightening means 5a such as squeezing (FIGS. 5 to 7). And the bag 3 which accommodated this fruit body piece 2 with the toothpick 4 is immersed in hot water using a pressing implement, and the bag 3 which accommodated the fruit body piece 2 with the toothpick 4 in this hot water is about 10 Sterilize for about 2 to 15 seconds (FIGS. 8 and 9). When the sterilization is completed, the bag 3 is taken out and the bag 3 near the fruit body piece 2 with the toothpick 4 is twisted and sealed. The excess bag 30 is cut about 1 cm away from the sealed portion (FIGS. 10 and 11). In addition, the killing depth of the bacteria by the sterilization for about 10 seconds to 15 seconds by the hot water sterilization is about 3 mm of the fruit body piece 2. Therefore, the inside of the fruiting body piece 2 of about 3 mm or more is viable, and the germs attached to the surface of the fruiting body piece 2 are almost completely killed (FIG. 12). After that, the bag 3 containing the sterilized toothpick 4 fruiting body piece 2 is placed in a dark place, and the required temperature for cultivating Mannentake is 27 ° C to 30 ° C. Cultivate in an environment of natural temperature because it is a growing period. The hyphae growth is temporarily stopped by the impact of the hot water sterilization, but under normal temperature atmosphere, the hyphae can be confirmed on the fruit body piece 2 on the first or second day, and then on the first or second day. Can confirm hyphae in the toothpick 4. It is the toothpick 4 with a mycelia that this hypha can be confirmed, and becomes the toothpick inoculum 40 for the original fungus (see FIG. 12).

  A method for producing a large number of the same type of toothpick inoculum 40a using this root fungus inoculum 40 will be described. Water is placed in an appropriate pan from the bottom of the pan about 5 cm, and a number of commercially available toothpick 4 are placed therein. For example, 500-1000 pieces and rice bran 20g are put (FIG. 13, FIG. 14). The reason for adding this rice bran is that even if the physical properties of the toothpick 4 are white strawberries or other materials that are not suitable for cultivation of the strawberries, the rice bran 4 is cultured by impregnating the rice straw etc. It is possible to change to physical properties suitable for the above. After boiling the pan containing 500 to 1000 toothpicks 4 and rice bran, heat it for about 10 minutes. When the rice bran nutrient penetrates into the toothpick 4, take it out and spread it on newspaper. Dry (FIG. 15). This drying is performed with a humidity of about 65%. After the drying, for example, the heads are aligned in units of 50, banded with a rubber band, put into the bag 3 and the bag 3 is evacuated and sealed to prevent invasion of germs into the bag 3 (FIG. 16). , FIG. 17). In a simple example of the method for extracting air, it is simple to twist about 2/3 above the bag 3 several times (about 3 times), and the air in the bag 3 is reliably extracted. There is an advantage that the air can be prevented from being contaminated by outside bacteria in the bag 3 during the sterilization described later (FIG. 18). This air is extracted, and a plurality of bags 3 containing 50 twisted toothpicks 4 are bundled (there may be a single bag), and the bundled upper part is tied with a rubber band (FIG. 19). The bundled bag 3 is immersed in about 5 cm of water contained in a pan, the lid of the pan is closed, and after boiling, it is sterilized for about 10 minutes (FIG. 20). After the sterilization, the bundled rubber bands are cut and the bags 3 taken out one by one are twisted, and the air in the bags 3 is removed and tied up (FIG. 21). Then, the excess bag 30 is cut from the vicinity of the tied up place (FIG. 22-1). The sterilized toothpick 400 accommodated in the bag 3 becomes the medium 6 (referred to as medium 6) (FIG. 22-2). The above operations such as taking out and cutting are performed on each bag 3. Then turn off the fire and lower the pan. Then, a germ invasion preventing means such as a germ invasion preventing tape 7 or a sponge is provided at an appropriate position of the medium 6 (FIG. 23). Adhering anti-bacterial invasion tape 7 to the sterilized bag medium 6 with the toothpick, and pulling out the original fungus inoculum 40 (mycelia infected toothpick) attached to the fruit body piece 2 and rinsing with clean water 8 for about 1 second (reason for rinsing) When the hyphae-infected toothpick touches the outside air, the germs floating in the air immediately attach to the medium 6 through the invasion prevention tape 7 for 7 days in the dark. The toothpick inoculum 40a is completed by culturing around 27 to 30 ° C. before and after (FIGS. 24 to 27). In this work, the toothpick inoculum 40a can be produced, which is extremely efficient, ideal for culturing the desired Kakuno Ganoderma lucidum, and opens the way for low cost and cheap provision to Ichii. There are features such as. The above situation could be confirmed in implementation and / or culture experiments. FIG. 28 shows a range in which the fruit body piece 2 can be sterilized by hot water treatment up to about 3 mm in a predetermined time (about 10 seconds to about 15 seconds), and this inside is a range in which the raw hyphae reproduce. The situation is shown in an enlarged view, but could be confirmed in the implementation and / or culture experiment.

  And, what can be used as the raw wood of bamboo shoots to pierce and cultivate this toothpick inoculum 40a is a hard material such as kunugi, oak, tochi, etc. The thickness is about 15 cm in diameter and the length is about 13 cm ( FIG. 29). This log 9 is provided with an incision 900 having a depth (depth at which the toothpick inoculum 40a can be inserted) and / or a width of about 3 mm in a body cutting method, and this incision 900 is provided at two or three places. Further, several holes (not shown) having a suitable depth and a diameter of about 3 mm are formed in the log 9. The notches 900 and / or holes are means for piercing the toothpick inoculum 40a (FIGS. 30 to 32). The processed log 9a thus configured is concealed with the bag 10 having a thickness of about 0.03 mm to about 0.04 mm with air permeability (FIG. 33). Then, the air in the bag 10 covering the processed log 9a is sucked using means such as a vacuum cleaner, and the excess bag 1000 is tied (twisted up). Tighten (FIG. 34). An appropriate number of the processed raw wood 9a which is fastened with the rubber band 11 and concealed with the bag 10 is put in a steamer 12 (steaming means), and the processed raw wood 9a is sterilized while being steamed under normal pressure. For example, sterilization is performed at about 98 ° C. for about 5 hours (FIG. 35). Although the steamed bag 10 and the processed log 9a are returned to room temperature, in the process of returning to normal temperature, the bag 10 is brought into close contact with the outer peripheral surface of the processed log 9a due to the slight contraction of the built-in air. (This is the result of sucking the air in the bag 10 by using a means such as a vacuum cleaner. See FIGS. 36 and 37). In a state where the bag 10 is in close contact with the processed log 9a, the germ invasion preventing tape 7 is attached to the cut-out portion. In this example, the invasion preventing tape 7 desirably has a thickness of 2 mm to 3 mm and an area of 1.5 cm square, so that invasion of miscellaneous bacteria can be prevented almost completely when the toothpick inoculum 40a is inserted. Next, an example of preventing the invasion of germs at the time of puncturing will be described. Since the toothpick inoculum 40a extracted from the medium 6 picks up germs in the air, the germs are removed by rinsing with purified water 8 for about 1 second. When piercing from the intrusion prevention tape 7, at least the germs and / or purified water 8, dust, etc. adhering to the toothpick inoculum 40a are squeezed out using the elastic pressure of the germ invasion prevention tape 7 and sealed into the sealed processed log 9a. Invasion of various germs and / or purified water 8 is prevented (FIGS. 40 to 42). Therefore, the germ invasion preventing tape 7 having a thickness and / or an area for ensuring the elastic compression is required. By the operation as described above, only pure hyphae are reproduced on the processed log 9a in the bag 10. Further, in order to prevent invasion of germs and / or outside air from the toothpick inoculum 40a, the excess toothpick 40b is cut off, and this portion is sealed 13 with an oil-based bond, an adhesive, or the like, whereby a cultured log 901 can be manufactured ( FIG. 43).

  Through the operations described above, a plurality of cultivated logs 901 are stacked and temporarily suspended. In this example, about 30 days to about 40 days have passed under conditions of about 27 ° C. to about 30 ° C. under the condition of being stacked and concealing the whole culture log 901 with the cover 14 to prevent direct sunlight. At this stage, the mycelium spreads in the cultured log 901, and the hypotenosis is completed. In addition, in this temporary sludge, pesticide-free cultivation is performed without watering, drugs, etc. in principle.

  Cultivation of Kakuno Ganoderma by the natural temperature of the present invention is carried out in the middle of April. Explaining the work, the bag 10 of the cultured raw wood 901 in which the mycelium is prevalent is removed and buried in the well-drained soil G at the end of the mouth. In order to avoid direct sunlight, the main form is shaded at a rate of 3 minutes light and 7 minutes dark by chilling. And in May, we see germination of fruiting body 1. In this period, the natural temperature changes drastically, but the stem grows with the rise in temperature, and the length of the stem is usually about 6 cm to 10 cm around May 20th. Around this time, the tip of the stem flattened and began to show the state of an umbrella. Before starting to show the state of this umbrella, when the stalk grew about 4 cm to 5 cm, it surrounded all of the strains that had been laid down using heat insulating material to make it almost 100% dark. This ganoderma does not open its umbrella in the dark, only the stem continues to grow. In many cases, if the darkness is made immediately after the lower part is formed, the fruiting body 1 germinates and continues to crawl on the plain as it is to grow unnaturally. Therefore, in the beginning, a shade of 3 minutes for light and 7 minutes for darkness is made, and then it rises to about 4 cm to 5 cm. When it grows in the darkness, Ganoderma continues to grow while branching. Some grow on one. Ganoderma that branches off is called Kakuno Ganoderma, and Ganoderma that grows by itself is called Ganoderma. The height of the building 20 (side wall) is preferably 60 cm or more. The roof 21 has a slope of about 15 °, and a slight slope is also formed on the ceiling 22 so that rainwater can flow into and sprinkle into the enclosure, and holes (not shown) are provided in various places. Devise to spray water. The roof 21 can be freely opened and closed by a hinge 210. And, for example, it is convenient to open and sprinkle water during dry weather, and it is useful because it can visually check the growth of Ganoderma. Thereafter, in July, the outside air has a minimum temperature of 25 ° C. and the maximum temperature exceeds 30 ° C., and the room temperature of the building 20 increases as the outside air changes. Therefore, a heat insulating material or the like is attached to the building 20 to alleviate this rise. For example, by utilizing this heat insulating material, the humidity can be maintained at about 85% to 95%, which is suitable for the growth of ganoderma. Therefore, it grows smoothly despite the rise in temperature during July. And after the length of the stem becomes 35 cm or more, the harvest is made around August 1 as a guideline. This cultivation is a cultivation method that can be performed in a garden or a field at home (FIGS. 44 to 45). Mass production is possible in this expansion.

  In addition, there is a method that can be cultivated easily even in a narrow place of a home, a veranda, or the like. For example, it is a method for cultivating Kakuno Reishi in a hut, and this plant pot strain is cultivated in the shade of each household. An example of this is described below. The body 23 is erected on the flower pot 24, and the frame body 23 is covered with vinyl 25, a blanket, a cloth or the like to create darkness, and the ceiling opening 250 is tied with a string 26 so that it can be opened when necessary. In this example, cultivation is performed using natural temperature and humidity, and if necessary, the vinyl 25 is opened and watered (FIGS. 46-1 and 46-2).

Schematic diagram showing an example of the whole fruit body of the present invention, which is Ganoderma Schematic diagram showing an example of fruit body pieces Schematic showing an example of many toothpicks Schematic diagram showing an example of a state in which several toothpicks sterilized with rubber bands are attached to the fruit body pieces The schematic diagram which showed an example of the state which accommodated several sterilized toothpicks and fruit body pieces in the bag The schematic diagram which showed an example of the state which vents the air of the bag which accommodated the fruiting body piece and several toothpicks Schematic diagram showing an example of a state in which a bag containing a fruiting body piece and several toothpicks is tied (bundled) Scale schematic diagram showing an example of an instrument (pot) to be heat sterilized The schematic diagram which showed an example of the state which immerses the bag which accommodated the fruiting body piece and several toothpicks in the pan shown in FIG. Schematic diagram showing an example of a sterilized bag containing several boiled toothpicks that have been boiled (hot water sterilized) The schematic diagram which showed an example of the state which cuts an excess bag after twisting and sealing the bag shown in FIG. FIG. 11 is a schematic diagram showing an example of a spruce inoculum for the original fungus in a state where an extra bag is cut after twisting and sealing the bag shown in FIG. The schematic diagram which showed an example of the pan for manufacturing several sterilized toothpicks of this invention The schematic diagram which showed an example of the boiling state of the pan shown in FIG. Schematic showing an example of the state of drying several sterilized toothpicks on newspaper Schematic diagram showing an example of a medium consisting of a number of sterilized toothpicks (a medium), in which several sterilized toothpicks are bundled with rubber bands. Schematic showing an example of the state in which the culture medium is contained in the bag Schematic showing an example of a state in which the bag containing the culture medium is twisted up The schematic diagram which showed an example of the state which bundled several bags which accommodated the culture medium created in FIG. The schematic diagram which showed an example of the state which puts the bag which accommodated the culture medium bundled in FIG. The schematic diagram which showed an example of the state which took out the bag which accommodated the culture medium sterilized with hot water from FIG. 20 from the pan. The schematic diagram which showed an example of the state which cut the excess location of the bag which accommodated the taken-out hot water sterilized culture medium Schematic showing an example of a medium consisting of sterilized toothpicks Schematic diagram showing an example of a state in which a germ invasion prevention tape is attached (provided) to the culture medium The schematic diagram which shows an example which opened the sterilized bag of FIG. 12, and took out the toothpick inoculum for original bacteria The schematic diagram which showed an example of the state rinsed with the purified water for the toothpick inoculum for the original bacteria taken out in FIG. The schematic diagram which showed an example of the state which pierces the culture medium the toothpick inoculum for the original bacteria rinsed in FIG. The schematic diagram which showed an example of the state which pierced the culture | cultivation inoculum for the original bacteria in FIG. 24 in the culture medium An enlarged schematic diagram showing an example of a region where various bacteria are killed by hot water treatment of fruit body pieces and a region of viable bacteria Schematic diagram showing an example of raw wood 29 is a schematic cross-sectional view showing an example of a state in which the raw wood shown in FIG. 29 is cut with a tool such as a saw. Cross-sectional schematic diagram showing an example of a state where cuts are made in several places of the raw wood Schematic diagram showing an example of a state where cuts are made in three locations of the raw wood Schematic diagram showing an example of a state in which processed raw wood is housed in a bag Schematic showing an example of a state where the processed log is covered with a bag Schematic showing an example of the state of steaming the processed wood covered with the bag with a steamer Schematic diagram showing an example of a state in which the processed wood covered with a bag is steamed Schematic showing an example of a state where the processed wood covered with the bag is steamed and the bag is in close contact with the processed raw wood Schematic diagram showing an example of a toothpick inoculum in the medium Schematic diagram showing an example of a state in which the toothpick inoculum in the medium is removed Schematic diagram showing an example of a state in which the toothpick inoculum extracted according to FIG. 39 is rinsed with purified water 40 is a schematic diagram showing an example of a state in which the toothpick inoculum rinsed with purified water is inserted from a germ invasion prevention tape affixed to a processed log. 40 is a schematic diagram showing an example of a state where the toothpick inoculum rinsed with purified water is stabbed from a germ invasion prevention tape affixed to a processed log. Fig. 41 is a schematic diagram showing an example of a state in which a cultured raw wood is made by inserting a toothpick inoculum into a processed raw wood, cutting off an excess toothpick and sealing it. Schematic diagram showing an example of stacking culture logs, concealing them with a cover, and performing a temporary work Schematic diagram showing an example of a state in which the cultured log is fully covered in a building provided outdoors Schematic diagram showing an example of a state in which the main work is carried out in a flower pot covering the cultured raw wood The top view of the state which opened the cover of FIGS. 46-1

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Fruiting body 2 Fruiting body piece 3 Bag 30 Extra bag 300 Sterilized bag 4 Toothpick 40 Toothpaste inoculum 40a Toothpaste inoculum 40b Extra toothpick 400 Sterilized toothpick 5 Fastener 5a Tightening means 6 Medium 7 Miscellaneous bacteria invasion prevention tape 8 Purified water 9 Raw wood 9a Processed raw wood 900 Cut 901 Cultured wood 901a Top surface 10 Bag 1000 Extra bag 11 Rubber band 12 Steamer 13 Seal 14 Cover 20 Building 21 Roof 210 Hinge 22 Ceiling 23 Frame 24 Flower pot 25 Vinyl 250 Opening part 26 String G Soil W Kazuno Ganoderma

Claims (4)

A fruit bag and several toothpicks are put in a bag with air permeability and sealed, and the bag with air permeability containing the fruit body pieces and several toothpicks is treated in hot water for several days. The hyphae are seeded with the hyphae, and the hyphae with hyphae are stabbed into another bag containing a large number of toothpicks having antimicrobial invasion prevention means such as antiseptic invasion tape / sponge, etc. A pesticide-free shiitake mushroom, shimeji mushroom, garlic mushroom, deer horned spirit that is configured to generate hyphae on a number of toothpicks in this separate bag while preventing the invasion of other bacteria into the other bag How to sow turf . A method for sowing seeds of shiitake mushrooms, shimeji mushrooms, garlic mushrooms , deer horned turf with no pesticides, wherein the toothpick with hyphae is rinsed with purified water for about 1 second when inserted through the means for preventing invasion of various bacteria according to claim 1. . Non-agricultural shiitake mushrooms, shimeji mushrooms, garlic mushrooms, deer horned spirits , wherein the processing time when processing the bag containing the fruit body pieces and several toothpicks according to claim 1 in hot water is approximately 10 to 15 seconds. How to sow turf . Collect shiitake mushrooms, shimeji mushrooms, mushrooms and deer horned turf that grow naturally in Yamano, remove the dirt of the fruit body and various foreign substances with paper etc., cut a part of the fruit body of this pure fungus to an appropriate size, Make a piece, and store this fruit piece together with one or several sterilized toothpicks with the nutrients of rice straw contained in a breathable bag, and attach this toothpick to the fruit body piece In addition to extracting air from the bag, the end of the extracted bag is bound with a rubber band, a string, and a tightening means of squeezing, and the bag containing the fruiting body piece with the toothpick is immersed in hot water. When the sterilization is completed and the sterilization is completed, the bag near the toothpick fruiting body piece is twisted and sealed, and then the bag containing the sterilized toothpick fruiting body piece is placed in a dark place and the environment of natural temperature Cultivated under to produce a spp.
In order to produce a large number of the same kind of toothpick inoculum for this original fungus, first, put a lot of toothpick and rice bran in a pot filled with water, boil the pot, and in this toothpick When the rice bran nutrient has permeated, after taking out and drying, this toothpick is housed in a bag, and the air in the bag is evacuated and sealed, preventing invasion of germs into the bag, and disinfecting with hot water. Since this sterilized toothpick becomes a culture medium, a bag containing this culture medium is provided with antimicrobial invasion prevention means such as tape and sponge, and the antimicrobial invasion prevention means is inserted into the antimicrobial invasion prevention means, Can be produced by cultivating the above-mentioned toothpick inoculum into the raw wood, and after producing the cultured raw wood, this cultured raw wood is stacked and suspended, and buried in the ground or in a flower pot. shiitake by pesticide-free, which is configured to be this face down in Mushroom, Ganoderma lucidum, Ganoderma lucidum Kazuno method of cultivation.

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