JP3967238B2 - Topical skin preparation - Google Patents

Description

【００４５】
実験例２ 発毛効果
服部らの方法［Ｊ．Ｄｅｒｍａｔｏｌｏｇｙ，１０，４５−５４（１９８３）］により、生後４５日のＣ３Ｈマウス背部毛をバリカンで刈り取り、この部分を２つに区切り、一方に実施例１及び２のローション１ｍＬを、他方に本発明に関わる抽出物を含まない従来のローション（比較例１）を１日１回塗布した。各部位の全体に発毛が認められるまでに要した日数で比較した。その結果、本発明に関わる抽出物を含まないローションの発毛が認められるまでの期間と比較して、実施例１及び２のローションではその期間が短縮された。またその他の実施例においても同様の効果が認められた。
【００４６】
実験例３ 使用試験
実施例１及び２のローション１及び２、実施例３のクリーム、比較例１の従来のローション及び比較例２の従来のクリームを用いて、女性３０人（２１〜４６才）を対象に１ヶ月間の使用試験を行った。使用後、肌のシワ、タルミの改善効果をアンケートにより判定した。
【００４７】
これらの試験結果を表２に示した。その結果、オタネニンジン及びサンシチニンジンの抽出物を含有する皮膚外用剤は優れたシワ、タルミの改善作用を示した。なお、試験期間中、皮膚トラブルは一人もなく、安全性においても問題なかった。また、処方成分の劣化についても問題なかった。
【００４８】
【表２】

【００４９】
実施例４〜９についても同様に使用試験を行ったところ、優れたシワ、タルミ等の改善作用を示した。
【００５０】
【発明の効果】
以上のことから、本発明のオタネニンジン及びサンシチニンジンの抽出物は、優れたＤＮＡ合成促進作用を有し、その抽出物を含有する皮膚外用剤は、老化防止効果及び育毛効果に優れていた。また、オタネニンジン及びサンシチニンジンの抽出物を併用することにより効果が相乗的に増加した。さらに、オタネニンジンの側根を使用することにより、あるいは、オタネニンジン及びサンシチニンジンを加熱処理することにより効果が増加した。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an external preparation for skin which contains one or two extracts selected from ginseng and sancinin ginseng, promotes DNA synthesis in cells, and is excellent in aging prevention and hair growth action.
[0002]
[Prior art]
In recent years, as a factor of skin aging such as a decrease in skin flexibility and elasticity, an increase in fine lines and dullness, a decrease in skin cell activity is considered, and components having cell activation have been studied. Also in hair, a decrease in the activity of hair matrix cells, hair follicles, and root sheath cells involved in hair growth has led to a loss of hair loss and hair growth rate, and many studies have been conducted.
[0003]
DNA, which is one of the biological components, is an essential component in cell growth, and DNA is replicated during cell division and plays an important role in the transmission of genetic information. In the cell growth phase, DNA increases, protein synthesis in the cell increases, and division is actively performed. Therefore, it is considered that by promoting the synthesis of DNA, the activity of the cells is increased and the proliferation is promoted.
[0004]
Conventionally, blood circulation promoting action is well known as the effectiveness of ginseng and sancinin (patent documents 1 and 2). Moreover, as a DNA synthesis promoter, β-casein of human milk or milk is known (Patent Document 3).
[0005]
[Patent Document 1]
JP-A-6-239758 [Patent Document 2]
JP 2001-39851 A [Patent Document 3]
JP-A-6-166615 [0006]
[Problems to be solved by the invention]
However, no particularly excellent drug based on the DNA synthesis promoting action has been found, and neither ginseng nor sanchinin has been reported.
[0007]
[Means for Solving the Problems]
Under these circumstances, as a result of intensive studies, the present inventors have found that extracts of ginseng and sancinin gins have an excellent DNA synthesis promoting action, and a skin external preparation containing these extracts has an anti-aging effect. And found that the hair-growth effect is excellent. In addition, it has been found that the combined use of the extract of ginseng and sanchinin ginseng synergistically increases the effect, and furthermore, the effect of using the side root part of ginseng and heat treatment of ginseng and sanchinin ginseng. As a result, the present invention has been completed.
[0008]
Panax ginseng (Lepidoptera, Panax ginseng) used in the present invention is a plant cultivated in various places in Japan, Korea, North Korea, and northern China. Mainly dried roots are treated as herbal medicines, and are used as herbal medicines for stomach digestion and health tonics. Herbal medicines have names such as ginseng, ginseng, ginseng, etc. Due to differences in production methods, dried ginseng after drying or blanching the roots, and dried red after steaming with steam and heat treatment It can be roughly divided into 3 In addition, there are other names such as carrot, white hair, red hair, etc. that gathered the side roots (whisker root) part of the root from the shape of the root, such as direct participation, semi-curvature, tune participation.
[0009]
Sancynin carrot (Arcoceae, scientific name Panax not-ginseng) is cultivated on a slope of alpine at an altitude of about 1500 m near Yunnan Province in southern China, and is used as a Kampo prescription medicine for hemostasis and nutritional tonic. Herbal medicines have names such as 37 ginseng, red ginseng, thirty seven, red ginseng, mountain lacquer, gold inversion, etc. Steamed and heat-treated and then dried roughly, it can be broadly divided into ripening ginseng and ginseng ginseng.
[0010]
The extract of ginseng and sanchinin carrot used in the present invention is extracted from part of the plant body such as leaves, stems, flowers, fruits, seeds and roots of the plant body or the whole plant. Preferably, the one obtained by extracting from the roots of ginseng root or sansity carrot is preferable. Since these are marketed as herbal medicines under the names of white ginseng and 37 ginseng, they can be used. Panax ginseng preferably uses the side root portion of the root. Moreover, it is preferable to heat-process the root of the ginseng root or the root of a sanchinin ginseng, for example, it is good to process at 70-150 degreeC for several hours under steam humidification, or to steam. Since these are commercially available as herbal medicines under the names of white hair, red hair, and mature ginseng, these may be used. The extraction method is not particularly limited, and for example, it may be a heat extraction or a room temperature extraction.
[0011]
Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (1,3-butylene glycol, propylene glycol). , Glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether) Etc.). Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, 1,3-butylene glycol and propylene glycol. These solvents may be used alone or in combination of two or more.
[0012]
The extract may be used as it is, or may be used after concentration, dilution and filtration treatment, decolorization with activated carbon, deodorization treatment, or the like, if necessary. Furthermore, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product.
[0013]
In the DNA synthesis promoter and skin external preparation of the present invention, the above extract may be used as it is, and within the range not impairing the effect of the extract, oils and fats, waxes, which are components used in the external preparation, Hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, UV absorbers, thickeners, pigments, antioxidants, whitening Components such as an agent and a chelating agent can be blended.
[0014]
In addition to the above components, a cell activator or a blood circulation promoter is preferably added. As cell activators and blood circulation promoters, pantothenic acid derivatives, vitamin E derivatives, menthol, hinokitiol, cephalatin, etc., placenta extracts, serum deproteinized extracts, spleen extracts and other animal-derived extracts and assembly extracts, Examples include plant-derived extracts such as rosemary extract, agaric extract, garlic extract, royal jelly, and the like. Particularly preferred are pantothenic acid derivatives, vitamin E derivatives, assembly extracts, and menthol.
[0015]
The DNA synthesis promoter and skin external preparation of the present invention can be used for cosmetics, quasi drugs, and pharmaceuticals. Examples of the dosage form include lotions, creams, emulsions, gels, and aerosols. Agents, essences, packs, cleaning agents, bath preparations, foundations, powders, lipsticks, ointments, poultices, tonics, hair creams, shampoos, rinses and the like that are applied to the skin and hair.
[0016]
The compounding amount of the DNA synthesis promoter used in the present invention is 0.0001% by weight or more, preferably 0.001 to 10% by weight, based on the total amount of the external preparation for skin of the present invention. If it is less than 0.0001% by weight, a sufficient effect is hardly expected. When the blending amount exceeds 10% by weight, the effect is hardly recognized and it is uneconomical. In addition, the addition method may be added in advance or during the production, and may be appropriately selected in consideration of workability.
[0017]
【Example】
Next, in order to describe the present invention in detail, examples of producing the extract used in the present invention, formulation examples and experimental examples of the present invention will be given as examples, but the present invention is not limited thereto. In the examples, the part of the amount is part by weight, and% is% by weight.
[0018]
Production Example 1 Hot Water Extract of Ginseng Root 800 g of purified water was added to 40 g of dried ginseng root and extracted at 95-100 ° C. for 2 hours, followed by filtration. The filtrate was concentrated, freeze-dried and heated with ginseng. 19 g of water extract was obtained.
[0019]
Production Example 2 50% Ethanol Extract of Ginseng Root 500 g of purified water and 500 mL of ethanol were added to 100 g of dried ginseng root, extracted for 7 days at room temperature, filtered, and the filtrate was concentrated to dryness to give ethanol of ginseng. 28 g of extract was obtained.
[0020]
Production Example 3 1,3-Butylene Glycol Extract of Panax Ginseng 200 ml of 1,3-butylene glycol was added to 20 g of dried ginseng leaves and stems, extracted at room temperature for 7 days, filtered, and 1,3-butylene glycol of ginseng 165 g of butylene glycol extract was obtained.
[0021]
Production Example 4 Hot Water Extract of Panax ginseng (heat-treated) Panax ginseng root was steamed at 90-100 ° C for 3 hours and then dried at 75-80 ° C for 10 days. After adding 800 mL of purified water to 40 g of this heat-treated ginseng and extracting at 95-100 ° C. for 2 hours, filtering, concentrating the filtrate and freeze-drying, 16 g of hot water extract of ginseng (heat-treated) is obtained. Obtained.
[0022]
Production Example 5 Hot Water Extract of Panax Ginseng (Side Root) 800 mL of purified water was added to 40 g of dry matter of Panax ginseng root, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and lyophilized. 12 g of hot water extract of ginseng (side root) was obtained.
[0023]
Production Example 6 Hot Water Extract of Panax ginseng (side root, heat treatment) The side roots of Panax ginseng were steamed at 90-100 ° C for 3 hours and then dried at 75-80 ° C for 10 days. After adding 800 mL of purified water to 40 g of this heat-treated ginseng side root and extracting it at 95-100 ° C. for 2 hours, filtering, concentrating the filtrate and freeze-drying, hot water of ginseng (side root, heat-treated) 12 g of extract was obtained.
[0024]
Production Example 7 Hot water extract of sanchinin carrot 800 ml of purified water was added to 40 g of dried citrus carrot root, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and lyophilized. As a result, 7.5 g of a hot water extract of sanchinin carrot was obtained.
[0025]
Production Example 8 50% ethanol extract of sanchinin ginseng 500 ml of purified water and 500 mL of ethanol were added to 100 g of dried citrus carrot root, extracted for 7 days at room temperature, filtered, and the filtrate was concentrated to dryness. Thus, 9.8 g of an ethanol extract of sansity carrot was obtained.
[0026]
Production Example 9 1,3-Butylene Glycol Extract of Sancytin Ginseng 200 mL of 1,3-butylene glycol was added to 20 g of dried citrus carrot leaves and stems, extracted at room temperature for 7 days, filtered, and sansiti 160 g of 1,3-butylene glycol extract of carrot was obtained.
[0027]
Manufacture example 10 Hot water extract of sanchinin carrot (heat-treated) 800 ml of purified water was added to 40 g of sanchinin carrot root heated at 121 ° C for 1 hour, extracted at 95-100 ° C for 2 hours, and then filtered. The filtrate was concentrated and freeze-dried to obtain 2.5 g of a hot water extract of sanchinin carrot (heat treatment).
[0028]
Production Example 11 50% ethanol extract of sanchinin carrot (heat treatment) 500 ml of purified water and 500 ml of ethanol were added to 100 g of sanchinin carrot root heated at 121 ° C. for 1 hour, extracted at room temperature for 7 days, and filtered. The filtrate was concentrated to dryness to obtain 6.2 g of ethanol extract of sanchinin carrot.
[0029]
Example 1 Lotion 1
Formulation Formulation 1. Panax ginseng (side root, heat treatment)
Hot water extract (Production Example 6) 0.1 part 2.1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5). Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 5.0
8). Pantothenic acid ethyl alcohol 0.1
9. Methyl paraoxybenzoate 0.1
10. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
11. Fragrance 0.1
12 Purified water 84.37
[Production method] Components 1 to 6 and 12 and components 7 to 11 are uniformly dissolved, mixed and filtered to obtain a product.
[0030]
Example 2 Lotion 2
In Example 1, lotion 2 was obtained by replacing the hot water extract of ginseng (side root, heat treatment) with the hot water extract of Sancytin carrot (heat treatment) (Production Example 10).
[0031]
Comparative Example 1 Conventional Lotion A conventional lotion was prepared by replacing the hot water extract of ginseng (side root, heat treatment) with purified water.
[0032]
Example 3 Cream Formulation Formulation 1. 1. 50 parts ethanol extract of ginseng (Production Example 2) 0.05 part Sancithin carrot 50% ethanol extract (Production Example 8) 0.05
3. Squalane 5.5
4). Olive oil 3.0
5). Stearic acid 2.0
6). Beeswax 2.0
7). Octyldodecyl myristate 3.5
8). Polyoxyethylene cetyl ether (20E.O.) 3.0
9. Behenyl alcohol 1.5
10. Glycerol monostearate2.5
11. Fragrance 0.1
12 Methyl paraoxybenzoate 0.2
13. Ethyl paraoxybenzoate 0.05
14.1,3-Butylene glycol 8.5
15. Purified water 68.05
[Manufacturing method] Components 1 to 10 are heated and dissolved and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 12 to 15 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 11 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.
[0033]
Comparative Example 2 Conventional Cream In Example 2, a 50% ethanol extract of ginseng and 50% ethanol extract of Sancynin carrot were replaced with purified water to obtain a conventional cream.
[0034]
Example 4 Emulsion Formulation Formulation 1. Sancithin carrot hot water extract (Production Example 7) 0.001 part2. Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5). Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. Purified water 73.199
[Manufacturing method] Components 2 to 8 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring.
[0035]
Example 5 Gel formulation Formulation amount 1. 1. 50% 1,3-butylene glycol extract of ginseng (Production Example 3) 1.0 part Ethanol 5.0
3. Vitamin E 0.1
4). Methyl paraoxybenzoate 0.1
5). Polyoxyethylene hydrogenated castor oil (60 EO) 0.1
6). Fragrance 0.1
7.1,3-Butylene glycol 5.0
8). Glycerin 5.0
9. Xanthan gum 0.1
10. Carboxyvinyl polymer 0.2
11. Potassium hydroxide 0.2
12 Purified water 83.1
[Production Method] Components 2 to 6 and Components 1 and 7 to 12 are uniformly dissolved, and both are mixed to obtain a product.
[0036]
Example 6 Pack formulation Hot water extract of Panax ginseng (heat treatment) (Production Example 4) 0.1 part2. Sanchinin carrot (heat treatment)
50% ethanol extract (Production Example 10) 0.1
3. Polyvinyl alcohol 12.0
4). Ethanol 5.0
5.1,3-Butylene glycol 8.0
6). Methyl paraoxybenzoate 0.2
7). Polyoxyethylene hydrogenated castor oil (20 EO) 0.5
8). Citric acid 0.1
9. Sodium citrate 0.3
10. Fragrance 0.1
11. Purified water 73.6
[Production Method] Components 1 to 11 are uniformly dissolved to obtain a product.
[0037]
Example 7 Foundation Formulation Formulation 1. 1. Hot water extract of ginseng (side root) (Production Example 5) 1.0 part Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate (20E.O.) 1.0
4). Polyoxyethylene cetyl ether (20E.O.) 2.0
5). Cetanol 1.0
6). Liquid lanolin 2.0
7). Liquid paraffin 3.0
8). Isopropyl myristate 6.5
9. Sodium carboxymethylcellulose 0.1
10. Bentonite 0.5
11. Propylene glycol 4.0
12 Triethanolamine 1.1
13. Methyl paraoxybenzoate 0.2
14 Titanium dioxide 8.0
15. Talc 4.0
16. Bengala 1.0
17. Yellow iron oxide 2.0
18. Fragrance 0.1
19. Purified water 60.1
[Production Method] Components 2 to 8 are heated and dissolved, and kept at 80 ° C. to obtain an oil phase. Swell component 9 well in component 19, then add components 1 and 10-13 and mix uniformly. To this, components 14 to 17 pulverized and mixed with a pulverizer are added, and the mixture is stirred with a homomixer and kept at 75 ° C. to obtain an aqueous phase. The oil phase is added to this aqueous phase with stirring, cooled, component 18 is added at 45 ° C., and cooled to 30 ° C. with stirring to give a product.
[0038]
Example 8 Bath preparation formulation 1. 50 parts ethanol extract of Sansan Carrot (heat treatment) (Production Example 11) 5.0 parts Sodium bicarbonate 50.0
3. Menthol 0.1
4). Yellow No. 202 (1) 0.01
5). Fragrance 0.1
6). Sodium sulfate 44.79
[Production Method] Components 1 to 6 are uniformly mixed to obtain a product.
[0039]
Example 9 Ointment Formulation Formulation 1. Panax ginseng hot water extract (Production Example 1) 0.01 part2. Sancithin carrot 50% 1,3-butylene glycol extract (Production Example 9) 0.5
3. Polyoxyethylene cetyl ether (30E.O.) 2.0
4). Glycerol monostearate 10.0
5). Liquid paraffin 5.0
6). Cetanol 6.0
7). Methyl paraoxybenzoate 0.1
8). Propylene glycol 10.0
9. Purified water 66.39
[Production Method] Components 3 to 6 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1, 2, and 7-9 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled to 30 ° C. with stirring to obtain a product.
[0040]
Example 10 Hair Tonic Formulation 1. Hot water extract of ginseng (side root, heat treatment) (Production Example 6) 2.0 parts Ethanol 60.0
3. Glycerin 2.0
4). Assembly extract 1.0
5). Hinokitiol 0.1
6). Purified water 34.9
[Production method] Components 1 to 6 are sufficiently mixed with stirring and filtered to obtain a product.
[0041]
Next, experimental examples will be given to explain the effects of the present invention in detail.
[0042]
Experimental Example 1 DNA synthesis promoting action 10 ⁵ human normal skin fibroblasts were seeded in each well of a 96-well plate, 5% CO _{2 in} 0.2 mL of Eagle's MEM medium containing 10% calf fetal serum, The cells were cultured at 37 ° C. for 4 days. And it replaced | exchanged for the Eagle's MEM culture medium which does not contain the serum which added the extract of the ginseng and the sancininjin of each density | concentration, and culture | cultivated for 24 hours. As a control, Eagle's MEM medium without serum was used. After completion of the culture, the amount of DNA synthesized in the cells was measured using a commercially available kit (manufactured by Roche Boehringer, 2-bromo-2′-deoxy-uridine labeled detection kit III) and calculated as a ratio to the control.
[0043]
The test results are shown in Table 1. As a result, the extract of ginseng and sancinin gins showed an excellent DNA synthesis promoting action, and the effect increased synergistically when used in combination. Moreover, the effect increased by using the side root part of Panax ginseng or heat-treating Panax ginseng and Sancynin ginseng.
[0044]
[Table 1]

[0045]
Experimental Example 2 Hair growth effect by Hattori et al. [J. Dermatology, 10, 45-54 (1983)], a 45-day-old C3H mouse back hair was clipped with a clipper, this part was divided into two parts, and 1 mL of the lotions of Examples 1 and 2 was applied to the other side and the present invention was applied to the other side. A conventional lotion (Comparative Example 1) containing no extract related to was applied once a day. The comparison was made based on the number of days required for hair growth to be recognized throughout each site. As a result, the period of the lotions of Examples 1 and 2 was shortened compared to the period until the hair growth of the lotion containing no extract according to the present invention was observed. Similar effects were also observed in other examples.
[0046]
Experimental Example 3 Usage Test 30 women (21 to 46 years old) using lotions 1 and 2 of Examples 1 and 2, cream of Example 3, conventional lotion of Comparative Example 1 and conventional cream of Comparative Example 2 A one-month use test was conducted on the subjects. After use, the effect of improving skin wrinkles and tarmi was determined by a questionnaire.
[0047]
The test results are shown in Table 2. As a result, the external preparation for skin containing the extract of ginseng and sanchinin carrot showed an excellent wrinkle and tarmi improving action. During the test period, there was no skin problem and there was no problem with safety. There was also no problem with the deterioration of the prescription ingredients.
[0048]
[Table 2]

[0049]
Examples 4 to 9 were also tested in the same manner, and showed excellent improving effects such as wrinkles and tarmi.
[0050]
【The invention's effect】
From the above, the extract of ginseng and sanchinin carrot of the present invention has an excellent DNA synthesis promoting action, and the skin external preparation containing the extract was excellent in the antiaging effect and the hair growth effect. Moreover, the effect increased synergistically by using the extract of Panax ginseng and Sancynin carrot together. Furthermore, the effect increased by using the side roots of ginseng, or by heat-treating ginseng and sanchinin.

Claims (1)

A DNA synthesis promoter comprising a heat-treated extract of ginseng and a heat-treated extract of sanchinin carrot.