JP3628196B2 - Antioxidant and composition containing the same - Google Patents

Description

【００２７】
［実施例１］（クリーム）
下記記載の配合量においてＢ成分をＡ成分に混合し、均一に加熱溶解して温度を８０℃にした。次いでＣ成分を注入撹拌混合した後、撹拌しながら３０℃まで冷却しクリームを得た。

【００２８】
［比較例１］（クリーム）
実施例１において成分（Ｂ）ウルティカ・カマドロイド（Ｕｌｔｉｃａ ｃｈａｍａｅｄｒｙｏｉｄｅｓ）抽出物を除いた以外はすべて実施例１と同様にして調製し、前述した各試験に使用した。
【００２９】
［実施例２］（二相型ローション）
下記記載の配合量においてＡ成分を室温にて均一に混合溶解し、Ｂ成分をゆっくり撹拌添加し二相型ローションを得た。

【００３０】
［比較例２］（二相型ローション）
実施例２において成分（Ｂ）ウルティカ・カマドロイド（Urtica chamaedryoides）抽出物を除いた以外はすべて実施例２と同様にして調製し、前述した各試験に使用した。
【００３１】
［試験例２］ 有用性評価試験
実施例１、２及び比較例１、２で調製された組成物の有用性は、以下に示す試験方法により試験した。
則ち、健康な女性（２５〜４０才）８０名を２０名ずつに４群に分け、それぞれ実施例１、２及び比較例１、２の試料を１日２回ずつ塗布し、塗布開始後３ヶ月後の老化防止効果（肌荒れ防止、皮膚の艶・張り）についてアンケート調査を行って評価した。アンケートの評価基準は、有効なものを「優」、やや有効なものを「良」、わずかに有効なものを「可」、無効なものを「不可」として、比較例と比較して評価を行った。
第２表（表２）に示すごとく、比較例１、２に比べ実施例１、２では良好な結果が得られた。
【００３２】
【表２】

【００３３】
［実施例３］（油性軟膏）
下記記載の配合量において各成分を混合し、８０℃まで加温し徐々に冷却し油性軟膏を得た。

【００３４】
［試験例３］
実施例３において調製された油性軟膏を試験例１の抗酸化効果の評価試験法に示す方法と同様の方法に準じ抗酸化試験を行った。その結果、該油性軟膏は（Ｂ）成分を配合しない以外全ての成分を含む油性軟膏と比べて、ＤＰＰＨラジカル消去作用に優れていた。
【００３５】
【発明の効果】
以上記載の如く、本発明の抗酸化剤を含む化粧品、医薬品、医薬部外品、食品等の組成物は、生体内に生成した活性酸素や過酸化脂質によって引き起こされる障害を抑制する効果がある。従って、健康上、美容上の障害についての治療に有効であり、さらに飲食品の安定化・保存性の向上にも有用である。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an antioxidant and a composition containing the antioxidant. Furthermore, this invention relates to the skin external preparation which can be utilized in field | areas, such as a pharmaceutical containing this antioxidant, a quasi-drug, and cosmetics.
[0002]
[Prior art]
In recent years, it has been clarified that active oxygen generated in a living body reacts with an unsaturated fatty acid to produce lipid peroxide and adversely affect the human body. For example, active oxygen acts on ischemic damage and radiation damage, lipid peroxide and its oxidative degradation products act on nucleic acids and proteins, causing arteriosclerosis, hypertension, vascular disorders caused by them, liver dysfunction, retinopathy Cause cataracts. Especially in the skin, since it is directly stimulated by environmental factors such as ultraviolet rays, it is easy to generate active oxygen, abnormal active pigmentation such as spots and freckles such as increased active oxygen concentration, lipid peroxide generation, inflammation, edema, The effects such as necrosis, wrinkles, and aging are remarkable.
[0003]
In addition, cosmetics, pharmaceuticals, foods and drinks, etc., often contain oils and fats, react with active oxygen during storage and use to produce lipid peroxides, resulting in reduced quality, reduced nutrition, and the human body. The emergence of toxicity has become a major problem.
[0004]
For this reason, in order to improve in vivo lipid peroxide abnormalities, research for searching for drugs having an antioxidant action has been widely performed. Typical examples include fat-soluble tocopherol (vitamin E) and water-soluble ascorbic acid (vitamin C) as natural product antioxidants, and BHT (3,5-tert-butyl-) as a synthetic antioxidant. 4-hydroxytolen) and BHA (2, (3) -tert-butyl-hydroxysol) are exemplified, but the effect is not satisfactory.
[0005]
On the other hand, many attempts have been made to obtain substances having a high antioxidant effect in order to improve in vivo lipid peroxide abnormalities, and various herbal medicine extracts have been disclosed (for example, Japanese Patent Laid-Open No. Hei 5- No. 246877, JP-A-8-92053, JP-A-8-301745, etc.).
[0006]
[Problems to be solved by the invention]
However, the herbal extract has a somewhat higher antioxidant effect than tocopherol and ascorbic acid, but the effect is not satisfactory. In addition, the synthetic antioxidants BHT and BHA have problems such as suspected carcinogenicity. Therefore, in addition to these antioxidants, there is a demand for highly safe substances that have the same means for inhibiting lipid peroxide production.
[0007]
[Means for Solving the Problems]
In view of such circumstances, the present inventors have conducted extensive research to improve the problems of the prior art, and as a result, surprisingly, the extraction of Urtica chamaedryoides, a plant belonging to the genus Nettle, is a genus of nettle. It was found that the product has strong free radical scavenging action and antioxidant action.
[0008]
That is, the present invention includes an antioxidant characterized by containing an extract of Urtica chamaedryoides, which is a plant belonging to the family Nettle, and cosmetics, foods, pharmaceuticals and the like containing the antioxidant. A composition is provided.
[0009]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, embodiments of the present invention will be described in detail.
Urtica chamaedryoides used in the invention are plants belonging to the genus Nettle, which is native to Mexico.
[0010]
The extract of Ultica chamaedryoides used in the present invention is obtained by extracting from a part or all of a plant body such as leaves, stems, flowers, seeds, fruits, bark, rhizomes and roots of the plant. It is what Preferably, one obtained by extraction from one or both of seeds and stems is good. In general, dried or live plants are used as they are or after being cut. As the extraction solvent to be used, 100 parts of the following extraction solvent is used for 5 to 50 parts per dry matter or dry matter equivalent of the live plant.
[0011]
The extraction solvent is generally water, lower monohydric alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohol (1,3-butylene glycol). , Propylene glycol, etc.), lower alkyl esters (ethyl acetate, etc.), hydrocarbons (benzene, hexane, pentane, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, dipropyl ether), acetonitrile, etc. Is mentioned. These solvents may be used alone or in combination of two or more. Preferably, one or two or more solvents among water or a water-soluble solvent (solvents that can be mixed with water in an arbitrary ratio. For example, ethanol, methanol, propylene glycol, etc.) may be used.
[0012]
The extraction method is not particularly limited, and the extraction is performed at room temperature or under heating. Examples of the method include normal extraction and Soxhlet extraction. Although there is no restriction | limiting in extraction time, Generally 1 hour-1 week are preferable.
[0013]
The extract may be used as it is, but can also be used after various treatments. For example, a concentrated solution obtained by concentrating these under normal pressure or reduced pressure, or a solid obtained by evaporating the solvent in the concentrated solution to dryness, a solid obtained by crystallization from the concentrated solution and then filtered and dried, or a concentrated solution are frozen. Examples include dried solids.
[0014]
Although the usage-amount (blending amount) per dry matter conversion as an antioxidant of the extract of Ultica chamaedryoides which concerns on this invention is not specifically limited, 0.001-20.0 weight% on the basis of a total amount (Hereinafter referred to as “wt%”), particularly 0.01 to 10 wt%.
[0015]
The composition containing the antioxidant of the present invention is characterized by blending the above-mentioned antioxidant, and its use is arbitrary, but it can be widely used in cosmetics, foods, pharmaceuticals, quasi drugs, toiletries, etc. Can do.
[0016]
The cosmetic form to which the present invention is applied is not particularly limited, and examples thereof include skin lotion, emulsion, cream, foundation, pack, lipstick, face wash, shampoo, rinse, hair tonic and the like. These cosmetics include various components generally used in cosmetics, that is, aqueous components, oily components, powder components, alcohols, esters, surfactants, moisturizers, whitening components, ultraviolet absorbers, thickeners, Components such as a colorant, a fragrance, an antioxidant, a pH adjuster, a chelating agent, and an antiseptic can be blended.
[0017]
The food to which the present invention is applied is not particularly limited, and can be obtained, for example, by adding a required amount of an extract to each food raw material as a general food and processing and producing it by a normal production method.
[0018]
The pharmaceutical form and quasi-drug to which the present invention is applied are not particularly limited in dosage form. For example, tablets, granules, capsules, oral preparations such as liquid medicine, ointments, poultices, creams, liquid preparations, etc. Examples include external preparations, sterile solutions, suspensions and other injections, bath preparations and the like. These medicaments can take the required unit dosage forms together with physiologically recognized vehicles, carriers, excipients, binders, stabilizers, flavoring agents and the like. For example, compositions for tablets and capsules include binders such as tragacanth, gum arabic, and gelatin, excipients such as microcrystalline cellulose, gelatinizing starch, swelling agents such as alginic acid, and lubricants such as magnesium stearate. An agent, a sweetener such as sucrose, lactose, and saccharin, a flavoring agent such as peppermint, red mono oil, and cherry can be mixed together and formulated by an ordinary method. In addition, sterile compositions for injection dissolve active substances in vehicles such as water for injection, naturally produced vegetable oils such as sesame oil, coconut oil, peanut oil, cottonseed oil, or synthetic fat vehicles such as ethyl oleate Or it can be formulated by the usual method of suspending. As an external preparation, vaseline, paraffin, fats and oils, lanolin, macrogol and the like are used as a base, and an ointment and a cream are prepared by a usual method.
[0019]
The topical skin preparation of the present invention means any dosage form that can be applied externally, such as lotion, milky lotion, cream, foundation, pack, essence, lipstick, face wash, gel, aerosol, ointment, poultice, Examples include those applied to the skin such as paste agents, plaster bath agents, and cleaning agents, and those applied to hair such as shampoos, rinses, treatments, and hair tonics. Moreover, the external preparation for skin of the present invention can be used for any of pharmaceutical use, pharmacy use and cosmetic use.
[0020]
The skin external preparation of the present invention includes an aqueous component, an oily component, a powder component, a wax, a fatty acid, an alcohol, an ester, a surfactant, a moisturizing agent, and a whitening component, which are components used in normal skin external preparations. UV absorbers, thickeners, colorants, fragrances, antioxidants, pH adjusters, chelating agents, preservatives and the like can be appropriately blended within the scope of achieving the object of the present invention.
[0021]
【Example】
Hereinafter, the present invention will be described in detail based on examples. The present invention is not limited to these.
[0022]
[Extraction Example 1] (Production of Urtica chamaedryoides extract I)
Extract 100 g of dried Urtica chamaedryoides with 1 L of methanol at room temperature for 24 hours, and then perform extraction twice in the same manner, then concentrate the extract together and dry to dryness. 6 g was obtained.
[0023]
[Extraction Example 2] (Production of Urtica chamaedryoides extract II)
100 g of dried Urtica chamaedryoides in 1 L of water / ethanol (1: 1, volume ratio) solvent, extracted with stirring at room temperature for 24 hours, filtered, and the filtrate was concentrated to dryness 8.3 g of a powdery solid was obtained.
[0024]
[Extraction Example 3] (Production of Urtica chamaedryoides Extract III)
100 g of dried Urtica chamaedryoides is extracted with 1 L of a mixture of 1,3-butylene glycol and water (1: 1) at room temperature for 10 days, filtered, and the filtrate is concentrated to dryness and dried. 10 g of a solid was obtained.
[0025]
[Test Example 1] DPPH radical scavenging test In order to examine the antioxidant ability of the extracts I to III, each extract was evaluated by the following DPPH radical scavenging test method. For comparison, the same evaluation was performed for a 50% ethanol extract of rosemary (scientific name: Rosmarinus officinalis), which is already known to have a radical scavenging action, and specimens 100 ug / ml, 10 ug / ml, The radical removal rate (%) at 1 ug / ml was determined.
The specific test method is as follows. Using the ethanol solution of Diphenyl-p-picrylhydradil (DPPH), which is a neutral radical, the effect of inhibiting the lipid radical chain reaction of the above extract was examined. Add 800 ul of ethanol and 20 ul of specimen to 800 ul of 250 mM acetate buffer (pH 5.5), and preincubate at 30 ° C. for 5 minutes. To this solution, add 400 ul of 250 uM DPPH / ethanol solution, leave it at 30 ° C. for 30 minutes, measure the absorbance at 517 nm, and then add 20 ul of 300 ug / ml BHT (3,5-tert-butyl-4-hydroxytolen). The absorbance when the DPPH radical is completely removed is measured. From this difference, the radical removal rate is determined. The results are shown in Table 1 (Table 1). It was found that the Urtica chamaedryoides extract of Extraction Examples 1, 2, and 3 has a DPPH radical scavenging action comparable to that of rosemary extract.
[0026]
[Table 1]

[0027]
[Example 1] (Cream)
In the blending amount described below, the B component was mixed with the A component, and uniformly heated and dissolved to a temperature of 80 ° C. Next, component C was injected and mixed with stirring, and then cooled to 30 ° C. with stirring to obtain a cream.

[0028]
[Comparative Example 1] (Cream)
The same procedure as in Example 1 was conducted except that the component (B) Ultica chamadroids extract was removed in Example 1, and used in each test described above.
[0029]
[Example 2] (two-phase lotion)
In the blending amounts described below, the A component was uniformly mixed and dissolved at room temperature, and the B component was slowly stirred and added to obtain a two-phase lotion.

[0030]
[Comparative Example 2] (Two-phase lotion)
Except for the component (B) extract of Urtica chamaedryoides in Example 2, everything was prepared in the same manner as in Example 2 and used in each test described above.
[0031]
[Test Example 2] Usability Evaluation Test The usefulness of the compositions prepared in Examples 1 and 2 and Comparative Examples 1 and 2 was tested by the following test method.
That is, 80 healthy women (25 to 40 years old) are divided into 4 groups of 20 people each, and the samples of Examples 1 and 2 and Comparative Examples 1 and 2 are applied twice a day, after the start of application. A questionnaire survey was conducted to evaluate the effect of preventing aging after 3 months (prevention of rough skin, gloss and tension of the skin). The evaluation criteria of the questionnaire are “Excellent” for valid, “Good” for slightly effective, “Yes” for slightly effective, and “No” for invalid, and compared with comparative examples. went.
As shown in Table 2 (Table 2), better results were obtained in Examples 1 and 2 than in Comparative Examples 1 and 2.
[0032]
[Table 2]

[0033]
[Example 3] (Oil-based ointment)
Each component was mixed in the blending amounts described below, heated to 80 ° C. and gradually cooled to obtain an oily ointment.

[0034]
[Test Example 3]
The oily ointment prepared in Example 3 was subjected to an antioxidant test according to the same method as shown in the test method for evaluating the antioxidant effect of Test Example 1. As a result, the oily ointment was superior in DPPH radical scavenging action compared to an oily ointment containing all components except that the component (B) was not blended.
[0035]
【The invention's effect】
As described above, compositions such as cosmetics, pharmaceuticals, quasi drugs, and foods containing the antioxidant of the present invention have an effect of suppressing damage caused by active oxygen and lipid peroxide generated in the living body. . Therefore, it is effective for the treatment of health and cosmetic disorders, and is also useful for the stabilization of food and drink and the improvement of storage stability.

Claims (3)

An antioxidant characterized by containing an extract of Urtica chamaedryoides. A composition comprising the antioxidant according to claim 1. A topical skin preparation comprising the antioxidant according to claim 1.