JP2022102858A - Corneum protein carbonylation inhibitor and external skin preparation for inhibiting carbonylation of corneum protein - Google Patents

Abstract

To provide corneum protein carbonylation inhibitor containing as active ingredient what is found out as highly safe plant extracts having excellent inhibitory action on corneum protein carbonylation, and to provide an external skin preparation for inhibiting carbonylation of corneum protein containing the plant extracts.SOLUTION: As an active ingredient of the corneum protein carbonylation inhibitor of the present invention, an extract from one or more plants selected from the group consisting of Zanthoxylum piperitum, Prunus persica, Rosa multiflora, Matricaria chamomilla, Rosa, and Morus alba are used. For an external skin preparation for inhibiting carbonylation of corneum protein of the present invention, an extract from one or more plants selected from the group consisting of Zanthoxylum piperitum, Prunus persica, Rosa multiflora, Matricaria chamomilla, Rosa, and Morus alba are compounded.SELECTED DRAWING: None

Description

The present invention relates to a stratum corneum protein carbonylation inhibitor containing a highly safe extract from a plant as an active ingredient, and a skin external preparation for suppressing stratum corneum carbonylation containing the extract.

In recent years, research on stratum corneum carbonylated proteins has been actively conducted in relation to skin aging and photoaging. Generally, carbonylated proteins are produced as a result of direct oxidation of _two NH groups of amino acid residues such as Lys, Arg, and Pro in the protein to form a carbonyl group, and lipid peroxides are oxidized to lipid peroxides. Is decomposed into a highly reactive aldehyde, which is sometimes produced by binding to a protein (see Patent Document 1). In the skin, it is considered that the protein may be directly oxidized, or the sebum on the surface of the skin may be oxidized by free radicals to generate lipid peroxide to initiate the oxidation of the protein. Once lipid peroxides are produced, the oxidation proceeds in a chain reaction, not only stimulating the skin surface, but also penetrating deep into the stratum corneum and damaging cells.

The outermost layer of the skin, the stratum corneum, is composed of corneocytes, 85% of which is composed of the protein keratin. In recent years, it is known that this keratin is carbonylated by oxidative stress (ultraviolet rays, cigarette smoke) and the like that the skin receives on a daily basis (see Non-Patent Document 1). Normally, keratin takes up a lot of water molecules, but carbonylation eliminates water molecules, and after carbonylation, keratin cannot take in water molecules, and this also means that the skin It is known that the skin is dried and the appearance of the skin is impaired (see Patent Document 2).

It is known that the increase in carbonylated protein in the stratum corneum causes the loss of water retention in the skin (see Patent Document 3) and the loss of flexibility and elasticity of the skin (see Patent Document 3). See Patent Document 4). Therefore, by preventing the carbonylation of the stratum corneum protein in the epidermis and reducing the degree of carbonylation thereof, it is useful for improving the water retention of corneocytes and improving the flexibility and elasticity of the skin. Conceivable.

Japanese Unexamined Patent Publication No. 2004-340935 Japanese Unexamined Patent Publication No. 2004-107269 Japanese Unexamined Patent Publication No. 2005-249672 Japanese Unexamined Patent Publication No. 2006-349372

Jens J. Thiele et al., THE JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol.113, No.3, 1999, p.335, 339

It is an object of the present invention to find out among highly safe plant extracts having an excellent keratin protein carbonylation inhibitory action, and to provide a horny protein carbonylation inhibitor containing them as an active ingredient. do.
Another object of the present invention is to provide a skin external preparation for suppressing carbonylation of horny layer protein, which contains a plant extract having an excellent effect of suppressing carbonylation of horny layer protein.

In order to solve the above problems, the stratum corneum protein carbonylation inhibitor of the present invention is extracted from one or more plants selected from the group consisting of Japanese pepper, peach, age, chamomile, rose and morus. It is characterized by using a substance as an active ingredient.
In addition, the external skin preparation for suppressing carbonylation of stratum corneum protein of the present invention contains an extract from one or more plants selected from the group consisting of Japanese pepper, peach, age, chamomile, rose and morus. It is characterized by having done it.

According to the present invention, it is possible to provide a horny layer protein carbonylation inhibitor and a keratin protein carbonylation inhibitor for external use on the skin, which are excellent in safety and action and effect.

Hereinafter, embodiments of the present invention will be described.
[Weapon protein carbonylation inhibitor]
The stratum corneum protein carbonylation inhibitor of the present embodiment contains an extract from one or more plants selected from the group consisting of Japanese pepper, peach, age vine, chamomile, rose and soy sauce as an active ingredient. Is.

Here, the "extract" in the present embodiment includes an extract obtained by using the above plant as an extraction raw material, a diluted solution or a concentrated solution of the extract, a dried product obtained by drying the extract, or these. Both crude and refined products are included.

The extraction raw materials used in this embodiment are Japanese pepper (scientific name: Zanthoxylum piperitum De Candolle), peach (scientific name: Prunus persica Batsch), agetsu (raw drug name), chamomile (scientific name: Matricaria chamomilla), rose and sohakuhi (raw drug name). ).

Zanthoxylum piperitum De Candolle is a plant belonging to the genus Zanthoxylum of the Rutaceae family, and is distributed in the Japanese archipelago and the Korean Peninsula, and can be easily obtained from these areas. Examples of the site that can be used as an extraction raw material include a leaf part, a stem part, a flower part, a bud part, a fruit part, a pericarp part, a fruit nucleus part, an above-ground part, or a mixture thereof, but preferably in the fruit part. be.

Peach (Prunus persica Batsch) is a deciduous small tree belonging to the genus Plum of the Rosaceae family, which has been cultivated for a long time in China and Japan, and can be easily obtained from these areas. Examples of the constituent parts of the peach that can be used as the extraction site include leaf part, stem part, flower part, bud part, fruit part, pericarp part, fruit nucleus part, seed part or a mixture thereof, but leaves are preferable. It is a department.

Agetsu (herbal medicine name) is a dried fruit of Neubara (scientific name: Rosa multiflora) belonging to the genus Rosa in the Rosaceae family. Neurose (Rosa multiflora) is a deciduous shrub distributed from southwestern Hokkaido to Kyushu and the Korean Peninsula, and ages, which are dried fruits of mature Neubara, can be easily obtained from these areas.

Chamomile (also known as chamomile) is a cold-tolerant annual plant belonging to the genus Mayweed of the Asteraceae family, originating from the region from Europe to West Asia and easily available from these regions. Examples of the portion that can be used as the extraction raw material include a leaf portion, a stem portion, a flower portion, a fruit portion, a root portion, and the like, and the flower portion is preferable.

Rose is a general term for plants belonging to the genus Rosa in the family Rosaceae, and many of them are cultivated in large quantities as horticultural species and are easily available. The roses that can be used in this embodiment include centifolia rose (Rosa centifolia, also known as rose), galica rose (Rosa gallica), canina rose (Rosa canina), Neubara (Rosa multiflora), damask rose (Rosa damascena), and hamanasu. (Rosa rugosa) and the like can be mentioned, and one type can be used alone or in combination of two or more types.

Among these, it is preferable to use canina rose, centifolia rose and damask rose, it is preferable to use two or more of these three types in combination, and it is particularly preferable to use three types in combination.
When three kinds of canina rose, centifolia rose and damask rose are used in combination, the usage ratio shall be 20 to 50% by mass of canina rose, centifolia rose and damask rose, respectively, in terms of the dry mass of the extraction raw material. , And more preferably 25 to 40% by mass, respectively.

The constituent parts of the rose that can be used as the extraction raw material are not particularly limited, and examples thereof include above-ground parts such as flower part, fruit part, leaf part, trunk part, bark part, root part, or a mixture of these parts. However, it is preferable to use the flower part and / or the fruit part. Further, when a canina rose is used as a rose, a fruit part is particularly preferable, when a centifolia rose is used, a flower part is preferable, and when a damask rose is used, a flower part is particularly preferable.

Morus alba (herbal name) is a dried root skin of morus alba (scientific name: Morus alba). Morus alba grows naturally or is cultivated in various parts of Japan and can be easily obtained from these areas. Morus alba root bark is used as the extraction raw material.

The above-mentioned plant extract can be obtained by drying the extraction raw material, crushing it as it is or using a coarse crusher, and subjecting it to extraction with an extraction solvent. The above-mentioned plant extraction raw materials may be subjected to the extraction treatment individually, or may be subjected to the extraction treatment in combination of two or more kinds of extraction raw materials.
Drying may be carried out in the sun or by using a commonly used dryer.

As the extraction solvent, either a polar solvent or a non-polar solvent may be used.
When a polar solvent is used, examples of the polar solvent include water, a hydrophilic organic solvent, etc., and these may be used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. preferable. When a polar solvent is used as the extraction solvent, pretreatment such as degreasing may be performed with a non-polar extraction solvent such as hexane, and the pretreatment may efficiently perform the extraction treatment with the polar solvent. be.

Water that can be used as an extraction solvent includes pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those subjected to various treatments. Treatments applied to water include, for example, purification, heating, sterilization, filtration, ion exchange, osmoregulation, buffering and the like. Therefore, the water that can be used as the extraction solvent in the present embodiment includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline and the like.

Hydrophilic organic solvents that can be used as the extraction solvent include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol and isopropyl alcohol; and carbon atoms such as 1,3-butylene glycol, propylene glycol and glycerin. Examples thereof include 2 to 5 polyhydric alcohols; lower aliphatic ketones having 3 to 6 carbon atoms such as acetone and methyl ethyl ketone.

When a mixed solution of two or more polar solvents is used as an extraction solvent, the mixing ratio thereof is arbitrary and can be appropriately adjusted. For example, when a mixed solution of water and a hydrophilic organic solvent is used as an extraction solvent, it is mixed at an arbitrary ratio, that is, more than 0: 100 and less than 100: 0 (volume ratio, hereinafter similarly referred to as the same). Can be used and can be adjusted as appropriate.
For example, when a mixed solution of water and a lower fatty alcohol is used as an extraction solvent, the mixing ratio (volume ratio) of water and the lower fatty alcohol can be 9: 1 or more, and further 7 : 3 or more, or the mixing ratio of water and lower fatty alcohol can be 1: 9 or less, and even 2: 8 or less. When a mixed solution of water and polyhydric alcohol is used, the mixing ratio of water and polyhydric alcohol can be 8: 2 or more or 1: 9 or less, and water and lower aliphatic ketones can be used. When a mixed solution of water and a lower aliphatic ketone is used, the mixing ratio of water and the lower aliphatic ketone can be 9: 1 or more or 2: 8 or less.

On the other hand, when a non-polar solvent is used as the extraction solvent, for example, aliphatic hydrocarbons such as petroleum ether, n-hexane, n-pentane, n-butane, n-octane, cyclohexane, squalane, and squalane; benzene and toluene. Aromatic hydrocarbons such as; halogenated hydrocarbons such as dichloromethane, chloroform, dichloroethane, carbon tetrachloride; chain ethers such as diethyl ether; edible oils such as rapeseed oil and soybean oil and diacylglycerol (DAG), Oils and fats such as medium-chain fatty acid oils; etc. can be exemplified, and these can be used alone or in combination of two or more.
Among these, aliphatic hydrocarbons and oils and fats are preferable, and squalane and squalene are preferable as the aliphatic hydrocarbons. As the squalene and squalene, those derived from sharks and those derived from plants (for example, olives) can be exemplified, but both can be used.

As the polar solvent, for example, a solvent having a relative permittivity of more than 12 at 20 ° C. can be used, and a solvent having a relative permittivity of 15 or more can be used.
Further, as the non-polar solvent, for example, a solvent having a relative permittivity of 12 or less at 20 ° C. can be used, and a solvent having a relative permittivity of 5 or less can be used.

In the present embodiment, the extraction solvent used for extracting Sansho, Momo, Agetsu and Sohakuhi is preferably a polar solvent, more preferably a mixed solvent of water and a hydrophilic organic solvent, and water and a lower aliphatic alcohol or polyhydric. A mixture with alcohol is particularly preferred.
On the other hand, as the extraction solvent used for extracting chamomile and roses, non-polar solvents are preferable, saturated or unsaturated hydrocarbons, edible oils and fats and oils are more preferable, and squalane is particularly preferable.

The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be carried out according to a conventional method. For example, the extraction material is immersed in an extraction solvent having an amount (mass ratio) of 5 to 50 times that of the extraction material, the soluble component is extracted at room temperature or under reflux heating, and then the extract is filtered to remove the extraction residue. You can get the liquid. Distilling off the solvent from the obtained extract gives a paste-like concentrate, and further drying the concentrate gives a dried product.

The sunshaw extract, peach extract, agetsu extract, chamomile extract, rose extract and soybean extract obtained as described above all have an excellent effect of suppressing stratum corneum protein carbonylation. Therefore, it can be used as an active ingredient of a stratum corneum protein carbonylation inhibitor. The sunshaw extract, peach extract, agetsu extract, chamomile extract, rose extract and soybean extract may be used alone as an active ingredient or a mixture of two or more as an active ingredient. The stratum corneum protein carbonylation inhibitor of the present embodiment can be used in a wide range of applications such as pharmaceuticals, quasi-drugs, cosmetics, and foods and drinks.

The stratum corneum protein carbonylation inhibitor of the present embodiment may consist only of sansho extract, peach extract, agetsu extract, chamomile extract, rose extract or soybean extract or a mixture thereof. However, it may be a formulation of sansho extract, peach extract, agetsu extract, chamomile extract, rose extract or soybean extract or a mixture thereof.

The stratum corneum protein carbonylation inhibitor of the present embodiment uses a pharmaceutically acceptable carrier such as dextrin or cyclodextrin or any other auxiliary agent according to a conventional method, and is powdery, granular, tablet-like, liquid or the like. It can be formulated into any dosage form of. At this time, as the auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring / odorant, and the like can be used. The stratum corneum protein carbonylation inhibitor can be used by blending with other compositions (for example, external skin preparations, oral compositions, etc. described later), and also used as ointments, external liquids, patches, etc. be able to.

When the stratum corneum protein carbonylation inhibitor of the present embodiment is formulated, the content of each of the sansho extract, peach extract, agetsu extract, chamomile extract, rose extract and soybean extract is particularly limited. It is not something that is done, and it can be set as appropriate according to the purpose.

The stratum corneum protein carbonylation inhibitor of the present embodiment contains, if necessary, other substances having a stratum corneum protein carbonylation inhibitory action (for example, natural extract, etc.), a sunshaw extract, a peach extract, etc. , Ageitsu extract, chamomile extract, rose extract or soybean extract can be blended and used as an active ingredient.

Examples of the administration method for the patient of the stratum corneum protein carbonylation inhibitor of the present embodiment include transdermal administration, oral administration, etc., and an appropriate method for prevention / treatment thereof is appropriately selected according to the type of disease. do it. In addition, the dose of the stratum corneum protein carbonylation inhibitor of the present embodiment may be appropriately increased or decreased depending on the type of disease, severity, individual difference of patients, administration method, administration period and the like.

The stratum corneum protein carbonylation inhibitor of the present embodiment is used for the stratum corneum protein carbonylation inhibitory action of the sansho extract, peach extract, ageitsu extract, chamomile extract, rose extract and soybean extract. It is possible to suppress the carbonylation of proteins, thereby improving the water retention of keratinocytes, and also improving the softness and elasticity of the skin. Furthermore, by suppressing the carbonylation of the stratum corneum protein, it is possible to suppress the dullness of the skin and obtain a skin-beautifying effect. However, the stratum corneum protein carbonylation inhibitor of the present embodiment can be used for all uses other than these uses, which are significant in exerting the stratum corneum protein carbonylation inhibitory action.

Further, since the horny layer protein carbonylation inhibitor of the present embodiment has an excellent horny layer protein carbonylation inhibitory action, it is suitable for blending, for example, in an oral composition, a skin external preparation described later, or the like. .. In this case, Sansho extract, peach extract, agetsu extract, chamomile extract, rose extract or soybean extract or a mixture thereof may be blended as they are, or sunshaw extract, peach extract, etc. A stratum corneum protein carbonylation inhibitor formulated from an agetsu extract, chamomile extract, rose extract or soybean extract or a mixture thereof may be blended.

Oral compositions are those that are less likely to harm human health and are ingested by oral or gastrointestinal administration in normal social life, such as foods and drinks, pharmaceuticals, quasi-drugs, etc. It is not limited to the classification of. Therefore, the "oral composition" in the present embodiment includes general foods, feeds, health foods, foods with health claims (foods for specified health use, foods with nutritional claims, foods with functional claims), and non-pharmaceutical products that are orally ingested. , Pharmaceuticals, etc. are widely included.

When the sunshaw extract, peach extract, agetsu extract, chamomile extract, rose extract or soybean extract or a mixture thereof are blended in an oral composition, the blending amount of the active ingredient in them is the purpose of use, symptoms. , Gender, etc. can be changed as appropriate, but in consideration of the general intake of the oral composition to be added, the daily extract intake for adults should be about 1 to 1000 mg. It is preferable to set it to. When the oral composition to be added is in the form of granules, tablets or capsules, the amount of Sansho extract, peach extract, agetsu extract, chamomile extract, rose extract and soybean extract is added. Converted to the solid content of the extract, peach extract, agetsu extract, chamomile extract, rose extract and soybean extract, it is usually 0.1 to 100% by mass or more with respect to the oral composition to be added, which is preferable. Is 1 to 100% by mass or more.

In addition, since the stratum corneum protein carbonylation inhibitor of the present embodiment each has an excellent stratum corneum protein carbonylation inhibitory action, it can be suitably used as a reagent for research on these action mechanisms.

The stratum corneum protein carbonylation inhibitor of the present embodiment is suitably applied to humans, but animals other than humans (for example, mice, rats, etc.) as long as the respective actions and effects are exhibited. It can also be applied to hamsters, dogs, cats, cows, pigs, monkeys, etc.).

[External skin agent for suppressing carbonylation of stratum corneum protein]
The external skin preparation for suppressing carbonylation of the stratum corneum according to the present embodiment contains an extract from one or more plants selected from the group consisting of Japanese pepper, peach, age, chamomile, rose and soybean. It was done.
As mentioned above, the Sansho extract, the peach extract, the agetsu extract, the chamomile extract, the rose extract and the soybean extract all have an excellent effect of suppressing the carbonylation of the stratum corneum protein. By blending with an external preparation for skin, it can be used as an external preparation for skin that is particularly suitable for use in suppressing carbonylation of stratum corneum protein.

Here, the external skin preparation is not limited in its classification, and includes a wide range of skin lotions, non-pharmaceutical products, pharmaceuticals, etc. that are used transdermally, and specifically, for example, an ointment. Examples include creams, milky lotions, lotions, beauty liquids, lotions, gels, beauty oils, packs, foundations, lip creams, bathing agents, hair tonics, hair lotions, shampoos, rinses, soaps, body shampoos and the like.

In the skin external preparation, the blending amount of each of the sansho extract, peach extract, agetsu extract, chamomile extract, rose extract and sohakuhi extract can be appropriately adjusted according to the type of the skin external preparation. However, a suitable blending ratio is 0.0001 to 10% by mass in terms of solid content of sansho extract, peach extract, agetsu extract, chamomile extract, rose extract and soybean extract, respectively. A particularly suitable compounding ratio is 0.001 to 1% by mass.

The external skin preparation for suppressing stratum corneum protein carbonylation according to the present embodiment has an inhibitory effect on stratum corneum protein carbonylation possessed by sansho extract, peach extract, agetsu extract, chamomile extract, rose extract and soybean extract. Main agents, auxiliaries or other ingredients used in the production of normal skin external preparations, such as astringents, bactericidal / antibacterial agents, whitening agents, UV absorbers, moisturizers, cell activators, anti-inflammatory agents, as long as they do not interfere with Anti-allergic agents, antioxidant / active oxygen removers, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances and the like can be used in combination. Such concomitant use may result in a more general product and may result in better than normally expected synergistic effects with other concomitant active ingredients.

The skin external preparation for suppressing stratum corneum protein carbonylation according to the present embodiment has an action of suppressing stratum corneum protein carbonylation possessed by sansho extract, peach extract, agetsu extract, chamomile extract, rose extract and soybean extract. Through this, it is possible to improve the water retention of corneocytes; improve the flexibility and elasticity of the skin; suppress the dullness of the skin, and the like.

Hereinafter, the present invention will be specifically described with reference to production examples and test examples, but the present invention is not limited to the following examples.

[Production Example 1] Production of Sansho Extract 1500 mL of a 70% ethanol aqueous solution was added to 100 g of a dried product of the peel of Sansho, heat-extracted at 80 to 90 ° C. for 2 hours with a reflux extractor, and filtered hot. The obtained extract was dried to obtain a Japanese pepper extract (7 g, sample 1).

[Production Example 2] Production of peach extract 1500 mL of a 30% 1,3-butylene glycol aqueous solution is added to 100 g of a dried peach leaf, and heat extraction is performed at 80 to 90 ° C. for 2 hours with a reflux extractor. It was filtered. The obtained extract was dried to obtain a peach extract (24 g, sample 2).

[Production Example 3] Production of Agetsu Extract 1500 mL of 50% ethanol aqueous solution was added to 100 g of dried fruit of Neubara (Ageitsu), heat-extracted at 80-90 ° C. for 2 hours with a reflux extractor, and filtered hot. .. The obtained extract was dried to obtain an Agetsu extract (16 g, sample 3).

[Production Example 4] Production of chamomile extract Squalane was added to 100 g of dried chamomile flower, extracted and filtered. The obtained extract was dried to obtain a chamomile extract (1 g, sample 4).

[Production Example 5] Production of 3 kinds of rose mixed extract 33 g of dried canina rose fruit, 33 g of dried centifolia rose flower, and 33 g of damask rose flower are mixed, and squalane is added. Extraction was performed and filtered. The obtained extract was dried to obtain a three-kind rose mixed extract (1 g, sample 5).

[Production Example 6] Production of Souhakuhi extract Add 1500 mL of 50% 1,3-butylene glycol aqueous solution to 100 g of dried mugwa root bark (Souhakuhi), and heat and extract at 80 to 90 ° C. for 2 hours with a reflux extractor. Was performed and filtered at the time of heat. The obtained extract was dried to obtain a soybean extract (7 g, sample 6).

[Test Example 1] Suppressive effect test on carbonylation of stratum corneum protein-1
The Japanese pepper extract (Sample 1) obtained in Production Example 1 was tested for its inhibitory effect on carbonylation of stratum corneum protein as follows.

The stratum corneum of the scalp, which had been degreased with acetone after shaving the back of the head, was peeled off and recovered by a tape stripping method using a keratin checker (manufactured by Asahi Biomed). The stratum corneum checker (sample) to which the stratum corneum is attached is immersed in 1 mL of an aqueous solution containing a test sample (sample 1, see Table 1 below for sample concentration) and 100 μmol / L of sodium hypochlorite (NaClO), and at 37 ° C. Processed for 16 hours. As a control, an aqueous solution containing NaClO and not containing a sample was used, and as no carbonylation treatment (blank), water containing no NaClO and no sample was used for the same treatment. For the obtained sample, the level of stratum corneum protein carbonylation was evaluated by the following method.

The sample was washed 3 times with 1 mL of 0.1 mol / L MES (2-morpholinoethane sulfonic acid-Na) buffer (pH 5.5), followed by 20 μmol / L fluorescent hydrazide (fluorescein-5-thiosemicarbazide (FTSC), AnaSpec). The sample was immersed in a 0.1 mol / L MES buffer (pH 5.5) containing (manufactured by the same company) and reacted at room temperature under light-shielded conditions for 3 hours to label the carbonyl group of the stratum corneum protein. After completion of the reaction, the cells were washed 3 times with 1 mL of PBS (-) buffer, and images were taken with an all-in-one fluorescence microscope (BZ-X800, manufactured by KEYENCE CORPORATION). The obtained image was analyzed using the attached image analysis software, and the fluorescence brightness per stratum corneum area was taken as the carbonylation level. From the obtained results, the stratum corneum protein carbonylation inhibition rate (%) was calculated by the following formula.

Weapon protein carbonylation inhibition rate (%) = {1- (AC) / (BC)} × 100
Each term in the formula represents the following.
A: Carbonylation level with NaClO treatment / addition of test sample B: Carbonylation level with NaClO treatment / no sample addition (control) C: Carbonylation level with no NaClO treatment / no sample addition (blank) Table 1 shows the results. Shown in.

As shown in Table 1, the Japanese pepper extract (Sample 1) effectively suppressed the carbonylation of the stratum corneum protein. It was also confirmed that the degree of the carbonylation inhibitory effect on the stratum corneum can be adjusted by the concentration of the Japanese pepper extract.

[Test Example 2] Suppressive effect test on carbonylation of stratum corneum protein-2
The peach extract (sample 2), the agetsu extract (sample 3), the chamomile extract (sample 4), the rose three-kind mixed extract (sample 5), and the soybean extract (sample) obtained in Production Examples 2 to 6 were obtained. Regarding 6), the inhibitory effect on stratum corneum protein carbonylation was tested as follows.

The stratum corneum on the inside of the upper arm, which is an unexposed portion, was peeled off and recovered by a tape stripping method using a stratum corneum checker (manufactured by Asahi Biomed). The stratum corneum checker (sample) to which the stratum corneum is attached is immersed in 1 mL of an aqueous solution containing a test sample (samples 2 to 6, see Table 2 below for sample concentration) and 20 μmol / L of sodium hypochlorite (NaClO), and 37 Treated at ° C. for 16 hours. As a control, an aqueous solution containing NaClO and not containing a sample was used, and as no carbonylation treatment (blank), water containing no NaClO and no sample was used for the same treatment. For the obtained sample, the level of stratum corneum protein carbonylation was evaluated by the following method.

The sample was washed twice with 1 mL of 0.1 mol / L MES (2-morpholinoethane sulfonic acid-Na) buffer (pH 5.5), followed by 20 μmol / L fluorescent hydrazide (fluorescein-5-thiosemicarbazide (FTSC), AnaSpec). The sample was immersed in a 0.1 mol / L MES buffer (pH 5.5) containing (manufactured by the same company) and reacted at room temperature under light-shielded conditions for 1 hour to label the carbonyl group of the stratum corneum protein. After completion of the reaction, the cells were washed 3 times with 1 mL of PBS (-) buffer, and images were taken with an all-in-one fluorescence microscope (BZ-X800, manufactured by KEYENCE CORPORATION). The obtained image was analyzed using the attached image analysis software, and the fluorescence brightness per stratum corneum area was taken as the carbonylation level. From the obtained results, the stratum corneum protein carbonylation inhibition rate (%) was calculated by the following formula.

Weapon protein carbonylation inhibition rate (%) = {1- (AC) / (BC)} × 100
Each term in the formula represents the following.
A: Carbonylation level with NaClO treatment / addition of test sample B: Carbonylation level with NaClO treatment / no sample addition (control) C: Carbonylation level with no NaClO treatment / no sample addition (blank) Table 2 shows the results. Shown in.

As shown in Table 2, peach extract (sample 2), agetsu extract (sample 3), chamomile extract (sample 4), rose three-kind mixed extract (sample 5), and soybean extract (sample 6). Effectively suppressed the carbonylation of the stratum corneum protein. It was also confirmed that the degree of the carbonylation inhibitory effect on the stratum corneum can be adjusted by the concentrations of the peach extract, the agetsu extract, the chamomile extract, the three roses mixed extract, and the soybean extract.

[Formulation Example 1]
A cream having the following composition was produced by a conventional method.
Sansho extract (Production Example 1) 0.05 g
Kujin extract 0.1g
Scutellaria extract 0.1g
Liquid paraffin 5.0g
Sarashi beeswax 4.0g
Squalene 10.0g
Cetanol 3.0g
Lanolin 2.0g
Stearic acid 1.0g
Polyoxyethylene sorbitan oleate (20E.O.) 1.5g
Glyceryl monostearate 3.0 g
Oil-soluble licorice extract 0.1g
1,3-butylene glycol 6.0 g
Methyl paraoxybenzoate 1.5g
Fragrance 0.1g
Purified water balance (total amount is 100 g)

[Formulation Example 2]
A milky lotion was produced by a conventional method according to the following composition.
Peach extract (Production Example 2) 0.01 g
Jojoba oil 4.00g
1,3-butylene glycol 3.00g
Arbutin 3.00g
Polyoxyethylene cetyl ether (20E.O.) 2.50g
Olive oil 2.00g
Squalan 2.00g
Cetanol 2.00g
Glyceryl monostearate 2.00 g
Polyoxyethylene sorbitan oleate (20E.O.) 2.00 g
Methyl paraoxybenzoate 0.15 g
Stearyl glycyrrhetinate 0.10 g
Yellow 杞 extract 0.10g
Dipotassium glycyrrhizinate 0.10 g
Ginkgo leaf extract 0.10g
Conchiolin 0.10g
Phellodendron extract 0.10g
Fragrance 0.05g
Purified water balance (total amount is 100 g)

[Formulation Example 3]
A beauty essence having the following composition was produced by a conventional method.
Agetsu extract (Production Example 3) 0.01 g
Chamomile extract 0.1g
Carrot extract 0.1g
Xanthan gum 0.3g
Hydroxyethyl cellulose 0.1g
Carboxyvinyl polymer 0.1g
1,3-butylene glycol 4.0 g
Dipotassium glycyrrhizinate 0.1 g
Glycerin 2.0g
Potassium hydroxide 0.25g
Fragrance 0.01g
Preservative (methyl paraoxybenzoate) 0.15 g
Ethanol 2.0g
Purified water balance (total amount is 100 g)

[Formulation Example 4]
A hair tonic having the following composition was produced by a conventional method.
Chamomile extract (Production Example 4) 0.4 g
Rose 3 kinds mixed extract (Production Example 5) 0.4 g
Tocopherol acetate Appropriate amount Sephalatin 0.002g
Isopropylmethylphenol 0.1g
Sodium hyaluronate 0.15g
Glycerin 15.0g
Ethanol 15.0g
Fragrance Appropriate amount Chelating agent (sodium edetate) Appropriate amount Preservative (hinokitiol) Appropriate amount Solubilizer (polyoxyethylene cetiether) Appropriate amount Purified water balance (total amount is 100 g)

[Formulation Example 5]
A shampoo having the following composition was produced by a conventional method.
Souhakuhi extract (Production Example 6) 0.5 g
Marjoram extract 1.0g
Plum fruit extract 0.2g
Coconut oil fatty acid methyl taurine sodium 10.0g
Coconut oil fatty acid amide propyl betaine 10.0g
Polyoxyethylene alkyl ether sodium sulfate 20.0 g
Coconut oil fatty acid diethanolamide 4.0g
Propylene glycol 2.0g
Perfume Appropriate amount Purified water balance (total amount is 100g)

The stratum corneum protein carbonylation inhibitor and the skin external preparation for inhibiting stratum corneum carbonylation of the present invention greatly improve the water retention of corneocytes; improve the flexibility and elasticity of the skin; suppress the dullness of the skin, and the like. I can contribute.

Claims (2)

A stratum corneum protein carbonylation inhibitor comprising an extract from one or more plants selected from the group consisting of Japanese pepper, peach, age vine, chamomile, rose and soybean hakuhi as an active ingredient. A skin external preparation for suppressing carbonylation of stratum corneum protein, which comprises an extract from one or more kinds of plants selected from the group consisting of Japanese pepper, peach, age, chamomile, rose and soybean.