JP2004107232A - Skin care preparation for external use - Google Patents
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- JP2004107232A JP2004107232A JP2002269949A JP2002269949A JP2004107232A JP 2004107232 A JP2004107232 A JP 2004107232A JP 2002269949 A JP2002269949 A JP 2002269949A JP 2002269949 A JP2002269949 A JP 2002269949A JP 2004107232 A JP2004107232 A JP 2004107232A
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Abstract
Description
【0001】
【発明の属する技術分野】
本発明は、オタネニンジン及びサンシチニンジンから選ばれる1種又は2種の抽出物を含有することにより、細胞中のDNAの合成を促進し、老化防止及び育毛作用に優れた皮膚外用剤に関する。
【0002】
【従来の技術】
近年、皮膚の柔軟性や弾力性の低下、小じわ、くすみの増加等の皮膚老化の要因として、皮膚細胞の活性低下が考えられ、細胞の活性化を有する成分が研究されている。また、毛髪においても、毛の成長にかかわる毛母細胞や毛包、毛根鞘の細胞の活性の低下が、脱毛や毛の成長速度の低下につながっており、多くの研究がされている。
【0003】
生体成分の一つであるDNAは、細胞の増殖においての必須成分であり、細胞分裂の際にDNAが複製され、遺伝情報の伝達に重要な役割をしている。細胞の増殖期においては、DNAが増え、細胞内での蛋白合成が高まり分裂が活発に行われる。したがって、DNAの合成を促進することで、細胞の活性が高まり、増殖が促進されると考えられる。
【0004】
従来、オタネニンジンやサンシチニンジンの有効性としては、血行促進作用がよく知られている(特許文献1、2)。また、DNA合成促進剤としては、人乳や牛乳のβ−カゼインが知られている(特許文献3)。
【0005】
【特許文献1】
特開平6−239758号
【特許文献2】
特開2001−39851号
【特許文献3】
特開平6−166615号
【0006】
【発明が解決しようとする課題】
しかしながら、DNAの合成促進作用に基づく特に優れた薬剤は見出されておらず、オタネニンジンやサンシチニンジンにも報告がない。
【0007】
【課題を解決するための手段】
このような事情により、本発明者らは鋭意検討した結果、オタネニンジン及びサンシチニンジンの抽出物が優れたDNA合成促進作用を有し、それらの抽出物を含有する皮膚外用剤が、老化防止効果及び育毛効果に優れていることを見出した。また、オタネニンジンとサンシチニンジンの抽出物を併用することにより相乗的に効果が増加することを見出し、さらには、オタネニンジンの側根部分を使用すること及びオタネニンジンとサンシチニンジンを加熱処理することにより効果が増加することを見出し、本発明を完成するに至った。
【0008】
本発明に用いるオタネニンジン(ウコギ科、学名Panax ginseng)は、日本、韓国、北朝鮮、中国北部の各地で栽培がされている植物である。おもに、根を乾燥したものが生薬として扱われており、漢方処方薬として、胃健消化薬、保健強壮薬に用いられる。生薬としては、人参、朝鮮人参、高麗人参等の名称があり、製造方法の違いから、根を乾燥又は湯通ししてから乾燥した白参と、蒸気で蒸して加熱処理をしてから乾燥した紅参に大別できる。その他、根の形状から直参、半曲参、曲参等、根の側根(ヒゲ根)部分を集めた髭人参、白毛、紅毛等の別名がある。
【0009】
また、サンシチニンジン(ウコギ科、学名Panax noto−ginseng)は、中国南部雲南省近郊の標高約1500mの高山の斜面で栽培され、漢方処方薬として、止血、滋養強壮薬に用いられる。生薬としては、三七人参、田七人参、三七、田七、山漆、金不換等の名称があり、製造方法の違いから、根をそのまま乾燥した三七人参、田七人参と、蒸気で蒸して加熱処理をしてから乾燥した熟三七人参、熟田七人参等に大別できる。
【0010】
本発明に用いるオタネニンジン及びサンシチニンジンの抽出物とは、植物体の葉、茎、花、果実、種子、根等の植物体の一部又は全草から抽出したものである。好ましくは、オタネニンジンの根、サンシチニンジンの根から抽出して得られるものが良い。これらはそれぞれ白参、三七人参の名称で生薬として市販されているのでこれを使用すれば良い。オタネニンジンは、好ましくは、根の側根部分を使用するのが良い。また、オタネニンジンの根又はサンシチニンジンの根は、加熱処理をするのが好ましく、例えば、蒸気での加湿下、70〜150℃で数時間処理したり、あるいは、蒸すと良い。これらのものは白毛、紅毛、熟三七人参の名称で生薬として市販されているのでこれらを使用すれば良い。その抽出方法は特に限定されず、例えば、加熱抽出したものであっても良いし、常温抽出したものであっても良い。
【0011】
抽出する溶媒としては、例えば、水、低級アルコール類(メタノール、エタノール、1−プロパノール、2−プロパノール、1−ブタノール、2−ブタノール等)、液状多価アルコール(1,3−ブチレングリコール、プロピレングリコール、グリセリン等)、ケトン類(アセトン、メチルエチルケトン等)、アセトニトリル、エステル類(酢酸エチル、酢酸ブチル等)、炭化水素類(ヘキサン、ヘプタン、流動パラフィン等)、エーテル類(エチルエーテル、テトラヒドロフラン、プロピルエーテル等)が挙げられる。好ましくは、水、低級アルコール及び液状多価アルコール等の極性溶媒が良く、特に好ましくは、水、エタノール、1,3−ブチレングリコール及びプロピレングリコールが良い。これらの溶媒は1種でも2種以上を混合して用いても良い。
【0012】
上記抽出物は、抽出した溶液のまま用いても良く、必要に応じて、濃縮、希釈及び濾過処理、活性炭等による脱色、脱臭処理等をして用いても良い。さらには、抽出した溶液を濃縮乾固、噴霧乾燥、凍結乾燥等の処理を行い、乾燥物として用いても良い。
【0013】
本発明のDNA合成促進剤及び皮膚外用剤には、上記抽出物をそのまま使用しても良く、抽出物の効果を損なわない範囲内で、外用剤に用いられる成分である油脂類、ロウ類、炭化水素類、脂肪酸類、アルコール類、エステル類、界面活性剤、金属石鹸、pH調整剤、防腐剤、香料、保湿剤、粉体、紫外線吸収剤、増粘剤、色素、酸化防止剤、美白剤、キレート剤等の成分を配合することができる。
【0014】
以上の成分に加えて、好ましくは、細胞賦活剤又は血行促進剤を加えると良い。細胞賦活剤及び血行促進剤としては、パントテン酸誘導体、ビタミンE誘導体、メントール、ヒノキチオール、セファラチン等、胎盤抽出物、血清除蛋白抽出物、脾臓抽出物等の動物由来の抽出物やセンブリ抽出物、ローズマリー抽出物、オウバク抽出物、ニンニク抽出物等の植物由来の抽出物、あるいはローヤルゼリー等が挙げられる。特に好ましくは、パントテン酸誘導体、ビタミンE誘導体、センブリ抽出物、メントールが良い。
【0015】
本発明のDNA合成促進剤及び皮膚外用剤は、化粧品、医薬部外品、医薬品のいずれにも用いることができ、その剤形としては、例えば、ローション、クリ−ム、乳液、ゲル剤、エアゾール剤、エッセンス、パック、洗浄剤、浴用剤、ファンデ−ション、打粉、口紅、軟膏、パップ剤、トニック、ヘアクリーム、シャンプー、リンス等の皮膚及び毛髪に適用されるものが挙げられる。
【0016】
本発明に用いるDNA合成促進剤の配合量は、本発明の皮膚外用剤全量に対し、0.0001重量%以上、好ましくは0.001〜10重量%の配合が良い。0.0001重量%未満では十分な効果は望みにくい。10重量%を越えて配合した場合、効果の増強は認められにくく不経済である。また、添加の方法については、予め加えておいても、製造途中で添加しても良く、作業性を考えて適宜選択すれば良い。
【0017】
【実施例】
次に本発明を詳細に説明するため、実施例として本発明に用いる抽出物の製造例、本発明の処方例及び実験例を挙げるが、本発明はこれに限定されるものではない。実施例に示す配合量の部とは重量部を、%とは重量%を示す。
【0018】
製造例1 オタネニンジンの熱水抽出物
オタネニンジンの根の乾燥物40gに精製水800mLを加え、95〜100℃で2時間抽出した後、濾過し、その濾液を濃縮し、凍結乾燥してオタネニンジンの熱水抽出物を19g得た。
【0019】
製造例2 オタネニンジンの50%エタノール抽出物
オタネニンジンの根の乾燥物100gに精製水500mL及びエタノール500mLを加え、常温で7日間抽出した後、濾過し、その濾液を濃縮乾固して、オタネニンジンのエタノール抽出物を28g得た。
【0020】
製造例3 オタネニンジンの1,3−ブチレングリコール抽出物
オタネニンジンの葉及び茎の乾燥物20gに1,3−ブチレングリコール200mLを加え、常温で7日間抽出した後、濾過し、オタネニンジンの1,3−ブチレングリコール抽出物を165g得た。
【0021】
製造例4 オタネニンジン(加熱処理)の熱水抽出物
オタネニンジンの根を90〜100℃で3時間蒸した後、75〜80℃で10日間乾燥させた。この加熱処理をしたオタネニンジン40gに精製水800mLを加え、95〜100℃で2時間抽出した後、濾過し、その濾液を濃縮し、凍結乾燥してオタネニンジン(加熱処理)の熱水抽出物を16g得た。
【0022】
製造例5 オタネニンジン(側根)の熱水抽出物
オタネニンジンの側根の乾燥物40gに精製水800mLを加え、95〜100℃で2時間抽出した後、濾過し、その濾液を濃縮し、凍結乾燥してオタネニンジン(側根)の熱水抽出物を12g得た。
【0023】
製造例6 オタネニンジン(側根、加熱処理)の熱水抽出物
オタネニンジンの側根を90〜100℃で3時間蒸した後、75〜80℃で10日間乾燥させた。この加熱処理をしたオタネニンジンの側根40gに精製水800mLを加え、95〜100℃で2時間抽出した後、濾過し、その濾液を濃縮し、凍結乾燥してオタネニンジン(側根、加熱処理)の熱水抽出物を12g得た。
【0024】
製造例7 サンシチニンジンの熱水抽出物
サンシチニンジンの根の乾燥物40gに精製水800mLを加え、95〜100℃で2時間抽出した後、濾過し、その濾液を濃縮し、凍結乾燥してサンシチニンジンの熱水抽出物を7.5g得た。
【0025】
製造例8 サンシチニンジンの50%エタノール抽出物
サンシチニンジンの根の乾燥物100gに精製水500mL及びエタノール500mLを加え、常温で7日間抽出した後、濾過し、その濾液を濃縮乾固して、サンシチニンジンのエタノール抽出物を9.8g得た。
【0026】
製造例9 サンシチニンジンの1,3−ブチレングリコール抽出物
サンシチニンジンの葉及び茎の乾燥物20gに1,3−ブチレングリコール200mLを加え、常温で7日間抽出した後、濾過し、サンシチニンジンの1,3−ブチレングリコール抽出物を160g得た。
【0027】
製造例10 サンシチニンジン(加熱処理)の熱水抽出物
121℃で1時間加熱処理をしたサンシチニンジンの根40gに精製水800mLを加え、95〜100℃で2時間抽出した後、濾過し、その濾液を濃縮し、凍結乾燥してサンシチニンジン(加熱処理)の熱水抽出物を2.5g得た。
【0028】
製造例11 サンシチニンジン(加熱処理)の50%エタノール抽出物
121℃で1時間加熱処理をしたサンシチニンジンの根100gに精製水500mL及びエタノール500mLを加え、常温で7日間抽出した後、濾過し、その濾液を濃縮乾固して、サンシチニンジンのエタノール抽出物を6.2g得た。
【0029】
実施例1 ローション1
[製造方法]成分1〜6及び12と、成分7〜11をそれぞれ均一に溶解し、両者を混合し濾過して製品とする。
【0030】
実施例2 ローション2
実施例1において、オタネニンジン(側根、加熱処理)の熱水抽出物をサンシチニンジン(加熱処理)の熱水抽出物(製造例10)に置き換えたものをローション2とした。
【0031】
比較例1 従来のローション
実施例1において、オタネニンジン(側根、加熱処理)の熱水抽出物を精製水に置き換えたものを従来のローションとした。
【0032】
実施例3 クリーム
[製造方法]成分1〜10を加熱溶解して混合し、70℃に保ち油相とする。成分12〜15を加熱溶解して混合し、75℃に保ち水相とする。油相に水相を加えて乳化して、かき混ぜながら冷却し、45℃で成分11を加え、さらに30℃まで冷却して製品とする。
【0033】
比較例2 従来のクリーム
実施例2において、オタネニンジンの50%エタノール抽出物及びサンシチニンジンの50%エタノール抽出物を精製水に置き換えたものを従来のクリームとした。
【0034】
実施例4 乳液
[製造方法]成分2〜8を加熱溶解して混合し、70℃に保ち油相とする。成分1及び10〜13を加熱溶解して混合し、75℃に保ち水相とする。油相に水相を加えて乳化して、かき混ぜながら冷却し、45℃で成分9を加え、さらに30℃まで冷却して製品とする。
【0035】
実施例5 ゲル剤
[製造方法]成分2〜6と、成分1及び7〜12をそれぞれ均一に溶解し、両者を混合して製品とする。
【0036】
実施例6 パック
[製造方法]成分1〜11を均一に溶解し製品とする。
【0037】
実施例7 ファンデーション
[製造方法]成分2〜8を加熱溶解し、80℃に保ち油相とする。成分19に成分9をよく膨潤させ、続いて、成分1及び10〜13を加えて均一に混合する。これに粉砕機で粉砕混合した成分14〜17を加え、ホモミキサーで撹拌し75℃に保ち水相とする。この水相に油相をかき混ぜながら加え、冷却し、45℃で成分18を加え、かき混ぜながら30℃まで冷却して製品とする。
【0038】
実施例8 浴用剤
[製造方法]成分1〜6を均一に混合し製品とする。
【0039】
実施例9 軟膏
[製造方法]成分3〜6を加熱溶解して混合し、70℃に保ち油相とする。成分1、2及び7〜9を加熱溶解して混合し、75℃に保ち水相とする。油相に水相を加えて乳化して、かき混ぜながら30℃まで冷却して製品とする。
【0040】
実施例10 ヘアトニック
[製造方法]成分1〜6を十分攪拌混合し、濾過して製品とする。
【0041】
次に、本発明の効果を詳細に説明するため、実験例を挙げる。
【0042】
実験例1 DNA合成促進作用
ヒト皮膚正常線維芽細胞を96穴プレートの各穴に105個播種し、10%子牛胎児血清を含むEagle‘s MEM培地 0.2mLにて5%CO2、37℃で4日間培養した。そして、各濃度のオタネニンジン及びサンシチニンジンの抽出物を添加した血清を含まないEagle‘s MEM培地に交換し、24時間培養した。コントロールには、血清を含まないEagle‘s MEM培地を用いた。培養終了後、細胞のDNA合成量を市販のキット(ロッシュ・ベーリンガー製、2−ブロモ−2’−デオキシ−ウリジン ラベル化検出キット III)を用いて測定し、コントロールに対する比率として算出した。
【0043】
これらの試験結果を表1に示した。その結果、オタネニンジン及びサンシチニンジンの抽出物は、優れたDNA合成促進作用を示し、併用することにより効果は相乗的に増加した。また、オタネニンジンの側根部位を使用することにより、あるいは、オタネニンジン及びサンシチニンジンを加熱処理することにより効果が増加した。
【0044】
【表1】
【0045】
実験例2 発毛効果
服部らの方法[J.Dermatology,10,45−54(1983)]により、生後45日のC3Hマウス背部毛をバリカンで刈り取り、この部分を2つに区切り、一方に実施例1及び2のローション1mLを、他方に本発明に関わる抽出物を含まない従来のローション(比較例1)を1日1回塗布した。各部位の全体に発毛が認められるまでに要した日数で比較した。その結果、本発明に関わる抽出物を含まないローションの発毛が認められるまでの期間と比較して、実施例1及び2のローションではその期間が短縮された。またその他の実施例においても同様の効果が認められた。
【0046】
実験例3 使用試験
実施例1及び2のローション1及び2、実施例3のクリーム、比較例1の従来のローション及び比較例2の従来のクリームを用いて、女性30人(21〜46才)を対象に1ヶ月間の使用試験を行った。使用後、肌のシワ、タルミの改善効果をアンケートにより判定した。
【0047】
これらの試験結果を表2に示した。その結果、オタネニンジン及びサンシチニンジンの抽出物を含有する皮膚外用剤は優れたシワ、タルミの改善作用を示した。なお、試験期間中、皮膚トラブルは一人もなく、安全性においても問題なかった。また、処方成分の劣化についても問題なかった。
【0048】
【表2】
【0049】
実施例4〜9についても同様に使用試験を行ったところ、優れたシワ、タルミ等の改善作用を示した。
【0050】
【発明の効果】
以上のことから、本発明のオタネニンジン及びサンシチニンジンの抽出物は、優れたDNA合成促進作用を有し、その抽出物を含有する皮膚外用剤は、老化防止効果及び育毛効果に優れていた。また、オタネニンジン及びサンシチニンジンの抽出物を併用することにより効果が相乗的に増加した。さらに、オタネニンジンの側根を使用することにより、あるいは、オタネニンジン及びサンシチニンジンを加熱処理することにより効果が増加した。[0001]
TECHNICAL FIELD OF THE INVENTION
TECHNICAL FIELD The present invention relates to an external preparation for skin which contains one or two extracts selected from Panax ginseng and Panax ginseng to promote the synthesis of DNA in cells, and is excellent in anti-aging and hair growth effects.
[0002]
[Prior art]
In recent years, a decrease in skin cell activity is considered as a factor of skin aging, such as a decrease in skin flexibility and elasticity, an increase in fine lines and dullness, and a component having cell activation has been studied. Also, in hair, a decrease in the activity of hair cells, hair follicles, and root sheath cells involved in hair growth leads to hair loss and a decrease in hair growth rate, and many studies have been made.
[0003]
DNA, which is one of the biological components, is an essential component in cell growth. DNA is replicated during cell division and plays an important role in transmission of genetic information. During the growth phase of a cell, DNA is increased, protein synthesis in the cell is increased, and division is actively performed. Therefore, it is considered that by promoting the synthesis of DNA, the activity of the cell is increased and the proliferation is promoted.
[0004]
Conventionally, blood circulation promoting effects are well known as the efficacy of Panax ginseng and Panax ginseng (Patent Documents 1 and 2). As a DNA synthesis promoter, β-casein of human milk or milk is known (Patent Document 3).
[0005]
[Patent Document 1]
JP-A-6-239758 [Patent Document 2]
JP 2001-39851 A [Patent Document 3]
JP-A-6-166615 [0006]
[Problems to be solved by the invention]
However, no particularly excellent drug based on the DNA synthesis-promoting action has been found, and there is no report on Panax ginseng or Panax ginseng.
[0007]
[Means for Solving the Problems]
Under these circumstances, the present inventors have conducted intensive studies. As a result, extracts of Panax ginseng and Panax ginseng have an excellent DNA synthesis promoting action, and a skin external preparation containing these extracts has an antiaging effect. And excellent hair-growth effect. In addition, it was found that the combined use of extracts of Panax ginseng and Panax notoginseng increases the effect synergistically.Furthermore, it is effective to use the side root portion of Panax ginseng and heat-treat Panax ginseng and Panax notoginseng. Increased, and completed the present invention.
[0008]
Panax ginseng used in the present invention is a plant cultivated in various places in Japan, Korea, North Korea, and northern China. Dried roots are mainly treated as crude drugs, and are used as Kampo prescription drugs for gastric digestives and health tonics. As crude drugs, there are names such as ginseng, ginseng, and ginseng.Due to differences in production methods, there are dried or blanched and dried white ginseng, and steamed with steam and dried red after heat treatment. Ginseng can be roughly divided into In addition, there are other names such as mustard ginseng, white hair, red hair, etc. that collect the side roots (whisker roots) of the root, such as direct ginseng, half ginseng, and ginseng, depending on the shape of the root.
[0009]
In addition, Panax notoginseng (scientific name: Panax noto-ginseng) is cultivated on an alpine slope at an altitude of about 1500 m near Yunnan Province in southern China, and is used as a Chinese medicine for hemostasis and tonic. As crude drugs, there are names such as 37 ginseng, 7 ginseng, 37 ginseng, 7 ginseng, mountain lacquer, and gold fissure. After being steamed and heat-treated, it can be roughly divided into dried matured ginseng and matured ginseng.
[0010]
The extracts of Panax ginseng and Panax ginseng used in the present invention are those extracted from a part of a plant such as leaves, stems, flowers, fruits, seeds and roots of the plant, or whole plants. Preferably, those obtained by extracting from the roots of Panax ginseng and Panax ginseng are good. These are marketed as crude drugs under the names of white ginseng and sunflower ginseng, respectively. Panax ginseng is preferably used in the side root portion of the root. The root of Panax ginseng or the root of Panax ginseng is preferably subjected to heat treatment. For example, it may be treated at 70 to 150 ° C. for several hours under steam humidification, or steamed. Since these are marketed as crude drugs under the names of white hair, red hair, and mature ginseng, they may be used. The extraction method is not particularly limited, and may be, for example, a heat-extracted one or a room-temperature-extracted one.
[0011]
Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol and the like), liquid polyhydric alcohols (1,3-butylene glycol, propylene glycol) , Glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether) Etc.). Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, 1,3-butylene glycol and propylene glycol. These solvents may be used alone or in combination of two or more.
[0012]
The extract may be used as it is, or may be used after concentration, dilution and filtration, decolorization with activated carbon, deodorization, etc., if necessary. Furthermore, the extracted solution may be subjected to a treatment such as concentration and drying, spray drying, and freeze drying to be used as a dried product.
[0013]
In the DNA synthesis promoter and the skin external preparation of the present invention, the above extract may be used as it is, and as long as the effect of the extract is not impaired, oils and fats, waxes, Hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, humectants, powders, ultraviolet absorbers, thickeners, pigments, antioxidants, whitening Components such as an agent and a chelating agent can be blended.
[0014]
In addition to the above components, it is preferable to add a cell activator or a blood circulation promoter. Cell activators and blood circulation promoters include pantothenic acid derivatives, vitamin E derivatives, menthol, hinokitiol, cephalatin, etc., placenta extracts, serum deproteinized extracts, spleen extracts and other animal-derived extracts and assembly extracts, Examples include plant-derived extracts such as rosemary extract, oak extract, and garlic extract, and royal jelly. Particularly preferred are pantothenic acid derivatives, vitamin E derivatives, assembly extracts, and menthol.
[0015]
The DNA synthesis promoter and the external preparation for skin of the present invention can be used in any of cosmetics, quasi-drugs, and pharmaceuticals. Examples of the dosage form include lotions, creams, emulsions, gels, and aerosols. Preparations, essences, packs, detergents, bath preparations, foundations, powders, lipsticks, ointments, cataplasms, tonics, hair creams, shampoos, rinses, etc., which are applied to the skin and hair.
[0016]
The amount of the DNA synthesis promoter used in the present invention is at least 0.0001% by weight, preferably 0.001 to 10% by weight, based on the total amount of the external preparation for skin of the present invention. If it is less than 0.0001% by weight, a sufficient effect is hardly expected. If the amount is more than 10% by weight, the effect is hardly recognized and it is uneconomical. The method of addition may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability.
[0017]
【Example】
Next, in order to explain the present invention in detail, examples of the production of the extract used in the present invention, prescription examples of the present invention, and experimental examples are given, but the present invention is not limited thereto. The term “parts by weight” in Examples means “parts by weight”, and “%” means “% by weight”.
[0018]
Production Example 1 Hot water extract of Panax ginseng 800 mL of purified water was added to 40 g of dried Panax ginseng, extracted at 95 to 100 ° C. for 2 hours, filtered, and the filtrate was concentrated, freeze-dried, and heat-dried Panax ginseng. 19 g of an aqueous extract was obtained.
[0019]
Production Example 2 50% Ethanol Extract of Panax ginseng 500 mL of purified water and 500 mL of ethanol were added to 100 g of dried panax ginseng at room temperature, extracted for 7 days at room temperature, filtered, and the filtrate was concentrated to dryness to give ethanol of Panax ginseng. 28 g of the extract was obtained.
[0020]
Production Example 3 1,3-butylene glycol extract of Panax ginseng 200 mL of 1,3-butylene glycol was added to 20 g of dried leaves and stems of Panax ginseng, extracted at room temperature for 7 days, filtered, and filtered to form 1,3-butylene glycol 1,3-butylene. 165 g of butylene glycol extract was obtained.
[0021]
Production Example 4 Hot water extract of Panax ginseng (heat treatment) Panax ginseng roots were steamed at 90 to 100 ° C for 3 hours, and then dried at 75 to 80 ° C for 10 days. 800 mL of purified water was added to 40 g of the heat-treated panax ginseng, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and freeze-dried to obtain 16 g of a hot-water extract of panax ginseng (heat treatment). Obtained.
[0022]
Production Example 5 Hot water extract of Panax ginseng (side root) 800 mL of purified water was added to 40 g of a dried product of Panax ginseng (lateral root), extracted at 95 to 100 ° C. for 2 hours, filtered, concentrated, and freeze-dried. 12 g of a hot water extract of Panax ginseng (lateral root) was obtained.
[0023]
Production Example 6 Hot water extract of Panax ginseng (lateral roots, heat treatment) The panax of Panax ginseng was steamed at 90 to 100 ° C for 3 hours, and then dried at 75 to 80 ° C for 10 days. 800 mL of purified water was added to 40 g of the heat-treated panax ginseng, extracted at 95-100 ° C. for 2 hours, filtered, concentrated, lyophilized, and lyophilized to obtain hot water of panax ginseng (lateral roots, heat-treated). 12 g of the extract was obtained.
[0024]
Production Example 7 Hot water extract of Panax ginseng 800 mL of purified water was added to 40 g of dried panax root, extracted at 95 to 100 ° C. for 2 hours, filtered, concentrated, and freeze-dried. Thus, 7.5 g of a hot water extract of Panax ginseng was obtained.
[0025]
Production Example 8 50% Ethanol Extract of Panax ginseng 500 mL of purified water and 500 mL of ethanol were added to 100 g of the dried root of Panax ginseng, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness. As a result, 9.8 g of an ethanol extract of Panax ginseng was obtained.
[0026]
Production Example 9 1,3-butylene glycol extract of Panax ginseng 200 mL of 1,3-butylene glycol was added to 20 g of dried leaves and stems of Panax notoginseng, extracted at room temperature for 7 days, filtered, and filtered. 160 g of 1,3-butylene glycol extract of carrot was obtained.
[0027]
Production Example 10 Hot Water Extract of Panax ginseng (Heat Treatment) 800 mL of purified water was added to 40 g of Panax ginseng heat-treated at 121 ° C. for 1 hour, extracted at 95-100 ° C. for 2 hours, and then filtered. The filtrate was concentrated and freeze-dried to obtain 2.5 g of a hot water extract of Panax ginseng (heat treatment).
[0028]
Production Example 11 50% Ethanol Extract of Panax ginseng (heat treatment) 500 mL of purified water and 500 mL of ethanol were added to 100 g of Panax ginseng heat-treated at 121 ° C. for 1 hour, extracted at room temperature for 7 days, and then filtered. The filtrate was concentrated to dryness to obtain 6.2 g of an ethanol extract of Panax ginseng.
[0029]
Example 1 Lotion 1
[Manufacturing method] Components 1 to 6 and 12 and components 7 to 11 are each dissolved uniformly, and both are mixed and filtered to obtain a product.
[0030]
Example 2 Lotion 2
Lotion 2 was obtained by replacing the hot water extract of Panax ginseng (lateral root, heat treatment) in Example 1 with the hot water extract of Sanshin ginseng (heat treatment) (Production Example 10).
[0031]
Comparative Example 1 A conventional lotion in which the hot water extract of Panax ginseng (lateral root, heat treatment) in Example 1 was replaced with purified water was used.
[0032]
Example 3 Cream
[Manufacturing method] Components 1 to 10 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. The components 12 to 15 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The oil phase is added to the water phase, emulsified, cooled while stirring, and the component 11 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.
[0033]
Comparative Example 2 Conventional cream A conventional cream was prepared by replacing the 50% ethanol extract of Panax ginseng and the 50% ethanol extract of Panax ginseng with purified water in Example 2 with purified water.
[0034]
Example 4 Emulsion
[Production method] Components 2 to 8 are dissolved by heating and mixed, and the mixture is kept at 70 ° C to obtain an oil phase. Components 1 and 10 to 13 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase, emulsified, cooled while stirring, and the component 9 is added at 45 ° C, and further cooled to 30 ° C to obtain a product.
[0035]
Example 5 Gel
[Production method] Components 2 to 6, and components 1 and 7 to 12 are each dissolved uniformly, and both are mixed to obtain a product.
[0036]
Example 6 Pack
[Production method] Components 1 to 11 are uniformly dissolved to obtain a product.
[0037]
Example 7 Foundation
[Production method] Components 2 to 8 are heated and dissolved, and kept at 80 ° C to obtain an oil phase. Swell component 9 to component 19, then add components 1 and 10-13 and mix uniformly. The components 14 to 17 crushed and mixed by a crusher are added thereto, and the mixture is stirred with a homomixer and kept at 75 ° C. to obtain an aqueous phase. The oil phase is added to the aqueous phase while stirring, cooled, and the component 18 is added at 45 ° C, and cooled to 30 ° C with stirring to obtain a product.
[0038]
Example 8 Bath agent
[Production method] Components 1 to 6 are uniformly mixed to obtain a product.
[0039]
Example 9 Ointment
[Production method] Components 3 to 6 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. Components 1, 2 and 7 to 9 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The oil phase is added to the water phase, emulsified, and cooled to 30 ° C. with stirring to obtain a product.
[0040]
Example 10 Hair tonic
[Production method] Components 1 to 6 are sufficiently stirred and mixed, and filtered to obtain a product.
[0041]
Next, experimental examples will be described in order to explain the effects of the present invention in detail.
[0042]
Experimental Example 1 DNA synthesis promoting action 10 5 normal human skin fibroblasts were seeded in each well of a 96-well plate, and 0.2% of Eagle's MEM medium containing 10% fetal calf serum, 5% CO 2 , The cells were cultured at 37 ° C for 4 days. Then, the mixture was replaced with Eagle's MEM medium containing no serum containing the extracts of Panax ginseng and Panax ginseng at each concentration and cultured for 24 hours. Eagle's MEM medium without serum was used as a control. After completion of the culture, the amount of DNA synthesis in the cells was measured using a commercially available kit (Roche Boehringer, 2-bromo-2'-deoxy-uridine labeling detection kit III) and calculated as a ratio to the control.
[0043]
The test results are shown in Table 1. As a result, the extracts of Panax ginseng and Panax ginseng showed an excellent DNA synthesis promoting action, and the effect was synergistically increased by using the extract in combination. Further, the effect was increased by using the lateral root site of Panax ginseng or by heat-treating Panax ginseng and Panax ginseng.
[0044]
[Table 1]
[0045]
Experimental Example 2 Method of hair growth effect Hattori et al. [J. According to Dermatology, 10, 45-54 (1983)], the back hair of a 45-day-old C3H mouse is clipped with a clipper, this part is divided into two parts, one part of the lotion of Examples 1 and 2 and the other part of the present invention. A conventional lotion containing no extract (Comparative Example 1) was applied once a day. The comparison was made based on the number of days required for hair growth to be observed at each site. As a result, the period of the lotions of Examples 1 and 2 was shortened compared to the period until hair growth of the lotion containing no extract according to the present invention was observed. Similar effects were observed in other examples.
[0046]
EXPERIMENTAL EXAMPLE 3 Use test 30 women (21 to 46 years old) using the lotions 1 and 2 of Examples 1 and 2, the cream of Example 3, the conventional lotion of Comparative Example 1, and the conventional cream of Comparative Example 2. Was subjected to a one-month use test. After use, the effect of improving skin wrinkles and tarmi was determined by a questionnaire.
[0047]
Table 2 shows the test results. As a result, the external preparation for skin containing the extracts of Panax ginseng and Panax ginseng showed an excellent action of improving wrinkles and tarmi. During the test, there was no skin trouble, and there was no problem in safety. In addition, there was no problem with the deterioration of the prescription components.
[0048]
[Table 2]
[0049]
When a use test was similarly performed for Examples 4 to 9, excellent improvement effects such as wrinkles and tarmi were shown.
[0050]
【The invention's effect】
From the above, the extracts of Panax ginseng and Panax ginseng of the present invention had an excellent DNA synthesis promoting action, and a skin external preparation containing the extract was excellent in antiaging and hair-growth effects. The effect was synergistically increased by using the extracts of Panax ginseng and Panax ginseng in combination. Furthermore, the effect was increased by using lateral roots of Panax ginseng or by heat-treating Panax ginseng and Panax ginseng.
Claims (8)
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006036751A (en) * | 2004-07-27 | 2006-02-09 | Byung-Su Lee | Hair-growing agent composition using chinese medicine material |
JP2008290966A (en) * | 2007-05-24 | 2008-12-04 | Michiharu Sasaki | Hair grower |
JP2009167121A (en) * | 2008-01-15 | 2009-07-30 | Nihon Kenko Shokuzai Kk | Hair-growing composition |
JP2018193314A (en) * | 2017-05-16 | 2018-12-06 | 日本メナード化粧品株式会社 | Apoptosis inhibitor |
JP2019099504A (en) * | 2017-12-01 | 2019-06-24 | 日本メナード化粧品株式会社 | Hair follicle cell activator |
JP2020097548A (en) * | 2018-12-19 | 2020-06-25 | 日本メナード化粧品株式会社 | Skin external and internal preparations containing panax ginseng and/or its extract |
-
2002
- 2002-09-17 JP JP2002269949A patent/JP3967238B2/en not_active Expired - Fee Related
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006036751A (en) * | 2004-07-27 | 2006-02-09 | Byung-Su Lee | Hair-growing agent composition using chinese medicine material |
JP2008290966A (en) * | 2007-05-24 | 2008-12-04 | Michiharu Sasaki | Hair grower |
JP2009167121A (en) * | 2008-01-15 | 2009-07-30 | Nihon Kenko Shokuzai Kk | Hair-growing composition |
JP2018193314A (en) * | 2017-05-16 | 2018-12-06 | 日本メナード化粧品株式会社 | Apoptosis inhibitor |
JP2019099504A (en) * | 2017-12-01 | 2019-06-24 | 日本メナード化粧品株式会社 | Hair follicle cell activator |
JP7082788B2 (en) | 2017-12-01 | 2022-06-09 | 日本メナード化粧品株式会社 | Hair follicle cell activator |
JP2020097548A (en) * | 2018-12-19 | 2020-06-25 | 日本メナード化粧品株式会社 | Skin external and internal preparations containing panax ginseng and/or its extract |
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