JP3756950B2 - 組換え型シチメンチョウヘルペスウイルス、及びその使用 - Google Patents
組換え型シチメンチョウヘルペスウイルス、及びその使用 Download PDFInfo
- Publication number
- JP3756950B2 JP3756950B2 JP50753196A JP50753196A JP3756950B2 JP 3756950 B2 JP3756950 B2 JP 3756950B2 JP 50753196 A JP50753196 A JP 50753196A JP 50753196 A JP50753196 A JP 50753196A JP 3756950 B2 JP3756950 B2 JP 3756950B2
- Authority
- JP
- Japan
- Prior art keywords
- hvt
- virus
- recombinant
- fragment
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241001502481 Meleagrid alphaherpesvirus 1 Species 0.000 title claims abstract description 203
- 241000701047 Gallid alphaherpesvirus 2 Species 0.000 claims abstract description 271
- 241000700605 Viruses Species 0.000 claims abstract description 194
- 241001529453 unidentified herpesvirus Species 0.000 claims abstract description 157
- 229960005486 vaccine Drugs 0.000 claims abstract description 87
- 230000003612 virological effect Effects 0.000 claims abstract description 44
- 102000004127 Cytokines Human genes 0.000 claims abstract description 28
- 108090000695 Cytokines Proteins 0.000 claims abstract description 28
- 230000003053 immunization Effects 0.000 claims abstract description 26
- 238000002649 immunization Methods 0.000 claims abstract description 5
- 108020004414 DNA Proteins 0.000 claims description 334
- 241000711404 Avian avulavirus 1 Species 0.000 claims description 143
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 123
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 122
- 229920001184 polypeptide Polymers 0.000 claims description 121
- 241000287828 Gallus gallus Species 0.000 claims description 117
- 230000000890 antigenic effect Effects 0.000 claims description 105
- 241000701063 Gallid alphaherpesvirus 1 Species 0.000 claims description 100
- 108091029865 Exogenous DNA Proteins 0.000 claims description 92
- 108091007433 antigens Proteins 0.000 claims description 81
- 208000015181 infectious disease Diseases 0.000 claims description 80
- 239000000427 antigen Substances 0.000 claims description 79
- 102000036639 antigens Human genes 0.000 claims description 77
- 108090000288 Glycoproteins Proteins 0.000 claims description 74
- 102000003886 Glycoproteins Human genes 0.000 claims description 70
- 230000002458 infectious effect Effects 0.000 claims description 69
- 241000711450 Infectious bronchitis virus Species 0.000 claims description 61
- 108010031571 Marek's disease virus glycoprotein B Proteins 0.000 claims description 51
- 108010005774 beta-Galactosidase Proteins 0.000 claims description 46
- 108700021117 chicken MGF Proteins 0.000 claims description 42
- 241000701024 Human betaherpesvirus 5 Species 0.000 claims description 41
- 108010050904 Interferons Proteins 0.000 claims description 40
- 102000014150 Interferons Human genes 0.000 claims description 36
- 102000005936 beta-Galactosidase Human genes 0.000 claims description 32
- 229940079322 interferon Drugs 0.000 claims description 31
- 241000700588 Human alphaherpesvirus 1 Species 0.000 claims description 25
- 101710081079 Minor spike protein H Proteins 0.000 claims description 21
- 108010059722 Viral Fusion Proteins Proteins 0.000 claims description 11
- 229940096437 Protein S Drugs 0.000 claims description 10
- 101710198474 Spike protein Proteins 0.000 claims description 10
- 108060003393 Granulin Proteins 0.000 claims description 7
- 101000678879 Homo sapiens Atypical chemokine receptor 1 Proteins 0.000 claims description 6
- 102100022717 Atypical chemokine receptor 1 Human genes 0.000 claims description 5
- 241000588724 Escherichia coli Species 0.000 claims description 5
- 101710133291 Hemagglutinin-neuraminidase Proteins 0.000 claims description 5
- 101710088235 Envelope glycoprotein C homolog Proteins 0.000 claims description 4
- 101900190364 Infectious laryngotracheitis virus Envelope glycoprotein B Proteins 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 239000012634 fragment Substances 0.000 abstract description 524
- 238000000034 method Methods 0.000 abstract description 149
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 105
- 239000013598 vector Substances 0.000 abstract description 99
- 230000037431 insertion Effects 0.000 abstract description 74
- 238000003780 insertion Methods 0.000 abstract description 74
- 208000006758 Marek Disease Diseases 0.000 abstract description 58
- 108090000623 proteins and genes Proteins 0.000 description 355
- 108091027544 Subgenomic mRNA Proteins 0.000 description 126
- 235000013330 chicken meat Nutrition 0.000 description 116
- 239000013612 plasmid Substances 0.000 description 102
- 241000702626 Infectious bursal disease virus Species 0.000 description 87
- 210000004027 cell Anatomy 0.000 description 87
- 150000001413 amino acids Chemical class 0.000 description 78
- 102000004169 proteins and genes Human genes 0.000 description 75
- 235000001014 amino acid Nutrition 0.000 description 72
- 235000018102 proteins Nutrition 0.000 description 71
- 241000701093 Suid alphaherpesvirus 1 Species 0.000 description 59
- 230000000692 anti-sense effect Effects 0.000 description 51
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 50
- 101150108190 US2 gene Proteins 0.000 description 48
- 244000144977 poultry Species 0.000 description 45
- 235000013594 poultry meat Nutrition 0.000 description 45
- 102000039446 nucleic acids Human genes 0.000 description 44
- 108020004707 nucleic acids Proteins 0.000 description 44
- 150000007523 nucleic acids Chemical class 0.000 description 44
- 244000052769 pathogen Species 0.000 description 39
- 101150066555 lacZ gene Proteins 0.000 description 38
- 230000001717 pathogenic effect Effects 0.000 description 37
- 238000004519 manufacturing process Methods 0.000 description 36
- 201000010099 disease Diseases 0.000 description 35
- 108020004511 Recombinant DNA Proteins 0.000 description 34
- 230000002103 transcriptional effect Effects 0.000 description 34
- 238000002474 experimental method Methods 0.000 description 33
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 31
- 102000004190 Enzymes Human genes 0.000 description 29
- 108090000790 Enzymes Proteins 0.000 description 29
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 27
- 241000271566 Aves Species 0.000 description 27
- 239000000523 sample Substances 0.000 description 27
- 241000700584 Simplexvirus Species 0.000 description 25
- 238000002255 vaccination Methods 0.000 description 25
- 230000014509 gene expression Effects 0.000 description 24
- 239000002299 complementary DNA Substances 0.000 description 23
- 102000005348 Neuraminidase Human genes 0.000 description 22
- 108010006232 Neuraminidase Proteins 0.000 description 22
- 238000001890 transfection Methods 0.000 description 22
- 241001465754 Metazoa Species 0.000 description 21
- 244000005700 microbiome Species 0.000 description 21
- 108091026890 Coding region Proteins 0.000 description 20
- 238000012761 co-transfection Methods 0.000 description 20
- 230000008488 polyadenylation Effects 0.000 description 20
- 206010044302 Tracheitis Diseases 0.000 description 19
- 108091008146 restriction endonucleases Proteins 0.000 description 19
- 241000283690 Bos taurus Species 0.000 description 18
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 18
- 241000283073 Equus caballus Species 0.000 description 18
- 238000003752 polymerase chain reaction Methods 0.000 description 18
- 230000000638 stimulation Effects 0.000 description 18
- 101150034814 F gene Proteins 0.000 description 17
- 208000003423 Mucocele Diseases 0.000 description 17
- 238000002105 Southern blotting Methods 0.000 description 17
- 101150050057 UL43 gene Proteins 0.000 description 17
- 238000006243 chemical reaction Methods 0.000 description 17
- 101500001532 Avian infectious bursal disease virus Capsid protein VP2 Proteins 0.000 description 16
- 101150008820 HN gene Proteins 0.000 description 16
- 230000006801 homologous recombination Effects 0.000 description 16
- 238000002744 homologous recombination Methods 0.000 description 16
- 208000010359 Newcastle Disease Diseases 0.000 description 15
- 230000028993 immune response Effects 0.000 description 15
- 239000002609 medium Substances 0.000 description 15
- 210000001519 tissue Anatomy 0.000 description 15
- 241000282414 Homo sapiens Species 0.000 description 14
- 101000645498 Alkalihalobacillus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4) Uncharacterized protein BpOF4_10220 Proteins 0.000 description 13
- 101001132313 Clostridium pasteurianum 34.2 kDa protein in rubredoxin operon Proteins 0.000 description 13
- 101000618325 Enterobacteria phage T4 Uncharacterized 12.4 kDa protein in mobB-Gp55 intergenic region Proteins 0.000 description 13
- 101000653284 Enterobacteria phage T4 Uncharacterized 9.4 kDa protein in Gp31-cd intergenic region Proteins 0.000 description 13
- 101710154606 Hemagglutinin Proteins 0.000 description 13
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 13
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 13
- 101710176177 Protein A56 Proteins 0.000 description 13
- 101000758676 Pyrococcus woesei Uncharacterized 24.7 kDa protein in gap 5'region Proteins 0.000 description 13
- 210000002950 fibroblast Anatomy 0.000 description 13
- 239000003550 marker Substances 0.000 description 13
- 238000010367 cloning Methods 0.000 description 12
- 239000000185 hemagglutinin Substances 0.000 description 12
- 238000011160 research Methods 0.000 description 12
- 238000012216 screening Methods 0.000 description 12
- 241000712461 unidentified influenza virus Species 0.000 description 12
- 102000053602 DNA Human genes 0.000 description 11
- 238000010276 construction Methods 0.000 description 11
- 210000001161 mammalian embryo Anatomy 0.000 description 11
- 239000012528 membrane Substances 0.000 description 11
- 239000008188 pellet Substances 0.000 description 11
- 230000010076 replication Effects 0.000 description 11
- 238000011144 upstream manufacturing Methods 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 238000001262 western blot Methods 0.000 description 11
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 10
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 239000007983 Tris buffer Substances 0.000 description 10
- 230000037430 deletion Effects 0.000 description 10
- 238000012217 deletion Methods 0.000 description 10
- 101150029683 gB gene Proteins 0.000 description 10
- 239000000463 material Substances 0.000 description 10
- 239000002773 nucleotide Substances 0.000 description 10
- 125000003729 nucleotide group Chemical group 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 208000024891 symptom Diseases 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 10
- 108010076504 Protein Sorting Signals Proteins 0.000 description 9
- 229940031416 bivalent vaccine Drugs 0.000 description 9
- 238000003776 cleavage reaction Methods 0.000 description 9
- 230000029087 digestion Effects 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 9
- 230000004044 response Effects 0.000 description 9
- 230000007017 scission Effects 0.000 description 9
- 241000712005 Bovine respirovirus 3 Species 0.000 description 8
- 241000948219 Histomonas Species 0.000 description 8
- 241001500351 Influenzavirus A Species 0.000 description 8
- 108020005202 Viral DNA Proteins 0.000 description 8
- 230000000120 cytopathologic effect Effects 0.000 description 8
- 239000003814 drug Substances 0.000 description 8
- 230000004927 fusion Effects 0.000 description 8
- 101150036031 gD gene Proteins 0.000 description 8
- 230000012010 growth Effects 0.000 description 8
- 230000003472 neutralizing effect Effects 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- 238000000746 purification Methods 0.000 description 8
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 8
- 241000588807 Bordetella Species 0.000 description 7
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 7
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 7
- 102000004889 Interleukin-6 Human genes 0.000 description 7
- 108010076039 Polyproteins Proteins 0.000 description 7
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 7
- 230000007503 antigenic stimulation Effects 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 238000004113 cell culture Methods 0.000 description 7
- 239000012153 distilled water Substances 0.000 description 7
- 241000894007 species Species 0.000 description 7
- 210000004989 spleen cell Anatomy 0.000 description 7
- 229920000936 Agarose Polymers 0.000 description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 238000002965 ELISA Methods 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 241000702670 Rotavirus Species 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 229940028617 conventional vaccine Drugs 0.000 description 6
- 239000013601 cosmid vector Substances 0.000 description 6
- 244000052637 human pathogen Species 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 6
- 230000003902 lesion Effects 0.000 description 6
- 239000011159 matrix material Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 230000001681 protective effect Effects 0.000 description 6
- 238000010839 reverse transcription Methods 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 241000701161 unidentified adenovirus Species 0.000 description 6
- 241000238876 Acari Species 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 5
- 241000711895 Bovine orthopneumovirus Species 0.000 description 5
- 108090000565 Capsid Proteins Proteins 0.000 description 5
- 102100023321 Ceruloplasmin Human genes 0.000 description 5
- 241000242722 Cestoda Species 0.000 description 5
- 108020004705 Codon Proteins 0.000 description 5
- 241000711573 Coronaviridae Species 0.000 description 5
- 241000223924 Eimeria Species 0.000 description 5
- 241000701081 Equid alphaherpesvirus 1 Species 0.000 description 5
- 108090001005 Interleukin-6 Proteins 0.000 description 5
- 241000244206 Nematoda Species 0.000 description 5
- 241001674048 Phthiraptera Species 0.000 description 5
- 241000607142 Salmonella Species 0.000 description 5
- 241000242541 Trematoda Species 0.000 description 5
- 101150093578 VP2 gene Proteins 0.000 description 5
- 206010046865 Vaccinia virus infection Diseases 0.000 description 5
- 201000011510 cancer Diseases 0.000 description 5
- 239000013592 cell lysate Substances 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 230000036961 partial effect Effects 0.000 description 5
- 239000002356 single layer Substances 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 208000007089 vaccinia Diseases 0.000 description 5
- 101900223469 Avian infectious bronchitis virus Membrane protein Proteins 0.000 description 4
- 101500001537 Avian infectious bursal disease virus Protease VP4 Proteins 0.000 description 4
- 241000282465 Canis Species 0.000 description 4
- 241000282472 Canis lupus familiaris Species 0.000 description 4
- 241000725585 Chicken anemia virus Species 0.000 description 4
- 108010062580 Concanavalin A Proteins 0.000 description 4
- 241000283086 Equidae Species 0.000 description 4
- 241000282326 Felis catus Species 0.000 description 4
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
- 239000012981 Hank's balanced salt solution Substances 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 241000701085 Human alphaherpesvirus 3 Species 0.000 description 4
- 102000013462 Interleukin-12 Human genes 0.000 description 4
- 102000000588 Interleukin-2 Human genes 0.000 description 4
- 241000606860 Pasteurella Species 0.000 description 4
- 229930182555 Penicillin Natural products 0.000 description 4
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 4
- 241000702263 Reovirus sp. Species 0.000 description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 4
- 108020004440 Thymidine kinase Proteins 0.000 description 4
- 208000036142 Viral infection Diseases 0.000 description 4
- HMNZFMSWFCAGGW-XPWSMXQVSA-N [3-[hydroxy(2-hydroxyethoxy)phosphoryl]oxy-2-[(e)-octadec-9-enoyl]oxypropyl] (e)-octadec-9-enoate Chemical compound CCCCCCCC\C=C\CCCCCCCC(=O)OCC(COP(O)(=O)OCCO)OC(=O)CCCCCCC\C=C\CCCCCCCC HMNZFMSWFCAGGW-XPWSMXQVSA-N 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000002238 attenuated effect Effects 0.000 description 4
- WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 4
- 206010006451 bronchitis Diseases 0.000 description 4
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 230000017188 evasion or tolerance of host immune response Effects 0.000 description 4
- 108020001507 fusion proteins Proteins 0.000 description 4
- 102000037865 fusion proteins Human genes 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 239000003102 growth factor Substances 0.000 description 4
- 206010022000 influenza Diseases 0.000 description 4
- 229940117681 interleukin-12 Drugs 0.000 description 4
- 229940100601 interleukin-6 Drugs 0.000 description 4
- 201000004792 malaria Diseases 0.000 description 4
- 229940031348 multivalent vaccine Drugs 0.000 description 4
- 229940049954 penicillin Drugs 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000001632 sodium acetate Substances 0.000 description 4
- 235000017281 sodium acetate Nutrition 0.000 description 4
- 239000001509 sodium citrate Substances 0.000 description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 4
- 229960005322 streptomycin Drugs 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 230000029812 viral genome replication Effects 0.000 description 4
- 230000009385 viral infection Effects 0.000 description 4
- 229960004854 viral vaccine Drugs 0.000 description 4
- 239000013603 viral vector Substances 0.000 description 4
- LINMATFDVHBYOS-MBJXGIAVSA-N (2s,3r,4s,5r,6r)-2-[(5-bromo-1h-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C=C12 LINMATFDVHBYOS-MBJXGIAVSA-N 0.000 description 3
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 3
- 239000005695 Ammonium acetate Substances 0.000 description 3
- 101500001538 Avian infectious bursal disease virus Capsid protein VP3 Proteins 0.000 description 3
- 102100031780 Endonuclease Human genes 0.000 description 3
- 241000282324 Felis Species 0.000 description 3
- 241000711549 Hepacivirus C Species 0.000 description 3
- 241000700721 Hepatitis B virus Species 0.000 description 3
- 229920000209 Hexadimethrine bromide Polymers 0.000 description 3
- 241000701074 Human alphaherpesvirus 2 Species 0.000 description 3
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 3
- 241000701027 Human herpesvirus 6 Species 0.000 description 3
- 102000015696 Interleukins Human genes 0.000 description 3
- 108010063738 Interleukins Proteins 0.000 description 3
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108700026244 Open Reading Frames Proteins 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 206010033799 Paralysis Diseases 0.000 description 3
- 241000286209 Phasianidae Species 0.000 description 3
- 241000223960 Plasmodium falciparum Species 0.000 description 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 241000282887 Suidae Species 0.000 description 3
- 241000282898 Sus scrofa Species 0.000 description 3
- 241000224526 Trichomonas Species 0.000 description 3
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 3
- 235000019257 ammonium acetate Nutrition 0.000 description 3
- 229940043376 ammonium acetate Drugs 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 239000013553 cell monolayer Substances 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 3
- 206010014599 encephalitis Diseases 0.000 description 3
- 235000020776 essential amino acid Nutrition 0.000 description 3
- 239000003797 essential amino acid Substances 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 239000012091 fetal bovine serum Substances 0.000 description 3
- 239000012894 fetal calf serum Substances 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 229940031551 inactivated vaccine Drugs 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 229940047124 interferons Drugs 0.000 description 3
- 229940047122 interleukins Drugs 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 229930182817 methionine Natural products 0.000 description 3
- 235000006109 methionine Nutrition 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 238000006386 neutralization reaction Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000000241 respiratory effect Effects 0.000 description 3
- 210000000952 spleen Anatomy 0.000 description 3
- -1 stem cell factor Proteins 0.000 description 3
- 238000007920 subcutaneous administration Methods 0.000 description 3
- 229940124597 therapeutic agent Drugs 0.000 description 3
- 102000003390 tumor necrosis factor Human genes 0.000 description 3
- 210000002845 virion Anatomy 0.000 description 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 101710186708 Agglutinin Proteins 0.000 description 2
- 102100022987 Angiogenin Human genes 0.000 description 2
- 102100021253 Antileukoproteinase Human genes 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 2
- 241000680578 Canid alphaherpesvirus 1 Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 102000019034 Chemokines Human genes 0.000 description 2
- 108010012236 Chemokines Proteins 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- 241000243990 Dirofilaria Species 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 102400001368 Epidermal growth factor Human genes 0.000 description 2
- 101800003838 Epidermal growth factor Proteins 0.000 description 2
- 241000230501 Equine herpesvirus sp. Species 0.000 description 2
- 102000003951 Erythropoietin Human genes 0.000 description 2
- 108090000394 Erythropoietin Proteins 0.000 description 2
- 241000701087 Felid alphaherpesvirus 1 Species 0.000 description 2
- 102000018233 Fibroblast Growth Factor Human genes 0.000 description 2
- 108050007372 Fibroblast Growth Factor Proteins 0.000 description 2
- 108090000368 Fibroblast growth factor 8 Proteins 0.000 description 2
- 241000700662 Fowlpox virus Species 0.000 description 2
- 101800000342 Glycoprotein C Proteins 0.000 description 2
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 2
- 108010092372 Granulocyte-Macrophage Colony-Stimulating Factor Receptors Proteins 0.000 description 2
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 2
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 2
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 2
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 2
- 101710146024 Horcolin Proteins 0.000 description 2
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 2
- 101900208483 Human parainfluenza 3 virus Fusion glycoprotein F0 Proteins 0.000 description 2
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 2
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 2
- 108010054267 Interferon Receptors Proteins 0.000 description 2
- 102000000589 Interleukin-1 Human genes 0.000 description 2
- 108010002352 Interleukin-1 Proteins 0.000 description 2
- 102100026018 Interleukin-1 receptor antagonist protein Human genes 0.000 description 2
- 101710144554 Interleukin-1 receptor antagonist protein Proteins 0.000 description 2
- 102000003814 Interleukin-10 Human genes 0.000 description 2
- 108090000174 Interleukin-10 Proteins 0.000 description 2
- 102000003815 Interleukin-11 Human genes 0.000 description 2
- 108090000177 Interleukin-11 Proteins 0.000 description 2
- 108010065805 Interleukin-12 Proteins 0.000 description 2
- 108010017515 Interleukin-12 Receptors Proteins 0.000 description 2
- 102000003816 Interleukin-13 Human genes 0.000 description 2
- 108090000176 Interleukin-13 Proteins 0.000 description 2
- 108010002350 Interleukin-2 Proteins 0.000 description 2
- 108010038453 Interleukin-2 Receptors Proteins 0.000 description 2
- 102000000646 Interleukin-3 Human genes 0.000 description 2
- 108010002386 Interleukin-3 Proteins 0.000 description 2
- 102000004388 Interleukin-4 Human genes 0.000 description 2
- 108090000978 Interleukin-4 Proteins 0.000 description 2
- 102100039897 Interleukin-5 Human genes 0.000 description 2
- 108010002616 Interleukin-5 Proteins 0.000 description 2
- 108010038501 Interleukin-6 Receptors Proteins 0.000 description 2
- 102000010782 Interleukin-7 Receptors Human genes 0.000 description 2
- 108010038498 Interleukin-7 Receptors Proteins 0.000 description 2
- 102000004890 Interleukin-8 Human genes 0.000 description 2
- 108090001007 Interleukin-8 Proteins 0.000 description 2
- 102000000585 Interleukin-9 Human genes 0.000 description 2
- 108010002335 Interleukin-9 Proteins 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 101710189395 Lectin Proteins 0.000 description 2
- 102000004058 Leukemia inhibitory factor Human genes 0.000 description 2
- 108090000581 Leukemia inhibitory factor Proteins 0.000 description 2
- 101710179758 Mannose-specific lectin Proteins 0.000 description 2
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 2
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 108090001074 Nucleocapsid Proteins Proteins 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 102000004140 Oncostatin M Human genes 0.000 description 2
- 108090000630 Oncostatin M Proteins 0.000 description 2
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 2
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 2
- 102100039277 Pleiotrophin Human genes 0.000 description 2
- 241000288906 Primates Species 0.000 description 2
- 101710194807 Protective antigen Proteins 0.000 description 2
- 241000125945 Protoparvovirus Species 0.000 description 2
- 108010082545 Secretory Leukocyte Peptidase Inhibitor Proteins 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 102000013275 Somatomedins Human genes 0.000 description 2
- 102000006601 Thymidine Kinase Human genes 0.000 description 2
- 102000004887 Transforming Growth Factor beta Human genes 0.000 description 2
- 108090001012 Transforming Growth Factor beta Proteins 0.000 description 2
- 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 description 2
- 101150009795 UL54 gene Proteins 0.000 description 2
- 101150081415 UL55 gene Proteins 0.000 description 2
- 101150036700 VP3 gene Proteins 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 230000006154 adenylylation Effects 0.000 description 2
- 239000000910 agglutinin Substances 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 108010072788 angiogenin Proteins 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000012148 binding buffer Substances 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 238000010804 cDNA synthesis Methods 0.000 description 2
- 210000003837 chick embryo Anatomy 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 210000000805 cytoplasm Anatomy 0.000 description 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000000539 dimer Substances 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000012149 elution buffer Substances 0.000 description 2
- 229940116977 epidermal growth factor Drugs 0.000 description 2
- 229940105423 erythropoietin Drugs 0.000 description 2
- 229940126864 fibroblast growth factor Drugs 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
- 208000002672 hepatitis B Diseases 0.000 description 2
- 230000003308 immunostimulating effect Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 102000002467 interleukin receptors Human genes 0.000 description 2
- 108010093036 interleukin receptors Proteins 0.000 description 2
- 229940076144 interleukin-10 Drugs 0.000 description 2
- 229940074383 interleukin-11 Drugs 0.000 description 2
- 229940076264 interleukin-3 Drugs 0.000 description 2
- 229940028885 interleukin-4 Drugs 0.000 description 2
- 229940100602 interleukin-5 Drugs 0.000 description 2
- 229940096397 interleukin-8 Drugs 0.000 description 2
- XKTZWUACRZHVAN-VADRZIEHSA-N interleukin-8 Chemical compound C([C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](NC(C)=O)CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCSC)C(=O)N1[C@H](CCC1)C(=O)N1[C@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC(O)=CC=1)C(=O)N[C@H](CO)C(=O)N1[C@H](CCC1)C(N)=O)C1=CC=CC=C1 XKTZWUACRZHVAN-VADRZIEHSA-N 0.000 description 2
- 229940118526 interleukin-9 Drugs 0.000 description 2
- 238000007918 intramuscular administration Methods 0.000 description 2
- 238000007912 intraperitoneal administration Methods 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 201000009837 laryngotracheitis Diseases 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229940053128 nerve growth factor Drugs 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 235000019515 salmon Nutrition 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- 239000013638 trimer Substances 0.000 description 2
- 229940031418 trivalent vaccine Drugs 0.000 description 2
- 102000003298 tumor necrosis factor receptor Human genes 0.000 description 2
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- QRXMUCSWCMTJGU-UHFFFAOYSA-L (5-bromo-4-chloro-1h-indol-3-yl) phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP([O-])(=O)[O-])=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-L 0.000 description 1
- 101150084750 1 gene Proteins 0.000 description 1
- KBDIEUYACKKLIJ-UHFFFAOYSA-N 2-isocyanatoguanidine Chemical compound NC(=N)NN=C=O KBDIEUYACKKLIJ-UHFFFAOYSA-N 0.000 description 1
- 108010055961 AGGCCT-specific type II deoxyribonucleases Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000272517 Anseriformes Species 0.000 description 1
- 108020004491 Antisense DNA Proteins 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 241000008921 Avian coronavirus Species 0.000 description 1
- 241001516406 Avian orthoreovirus Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000588779 Bordetella bronchiseptica Species 0.000 description 1
- 241000588832 Bordetella pertussis Species 0.000 description 1
- 241000589968 Borrelia Species 0.000 description 1
- 241000589969 Borreliella burgdorferi Species 0.000 description 1
- 241000701083 Bovine alphaherpesvirus 1 Species 0.000 description 1
- 241000710780 Bovine viral diarrhea virus 1 Species 0.000 description 1
- 241001260012 Bursa Species 0.000 description 1
- 241000589876 Campylobacter Species 0.000 description 1
- 241000701101 Canine adenovirus 1 Species 0.000 description 1
- 235000014653 Carica parviflora Nutrition 0.000 description 1
- 108090000994 Catalytic RNA Proteins 0.000 description 1
- 102000053642 Catalytic RNA Human genes 0.000 description 1
- 241000606161 Chlamydia Species 0.000 description 1
- 241000710777 Classical swine fever virus Species 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 description 1
- 208000000655 Distemper Diseases 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241000709661 Enterovirus Species 0.000 description 1
- 241000991587 Enterovirus C Species 0.000 description 1
- 108010008655 Epstein-Barr Virus Nuclear Antigens Proteins 0.000 description 1
- 241000701089 Equid alphaherpesvirus 4 Species 0.000 description 1
- 241001452028 Escherichia coli DH1 Species 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- 241000713800 Feline immunodeficiency virus Species 0.000 description 1
- 241000714165 Feline leukemia virus Species 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 101000609762 Gallus gallus Ovalbumin Proteins 0.000 description 1
- 102000034354 Gi proteins Human genes 0.000 description 1
- 108091006101 Gi proteins Proteins 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 101710181600 Glycoprotein gp2 Proteins 0.000 description 1
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 1
- 102000044467 Grifin Human genes 0.000 description 1
- 108700036695 Grifin Proteins 0.000 description 1
- 241000606790 Haemophilus Species 0.000 description 1
- 241000150562 Hantaan orthohantavirus Species 0.000 description 1
- 208000007514 Herpes zoster Diseases 0.000 description 1
- 241000700586 Herpesviridae Species 0.000 description 1
- 229940124841 Herpesvirus vaccine Drugs 0.000 description 1
- 241000701109 Human adenovirus 2 Species 0.000 description 1
- 241000701041 Human betaherpesvirus 7 Species 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 101150027427 ICP4 gene Proteins 0.000 description 1
- 206010062016 Immunosuppression Diseases 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 102000008070 Interferon-gamma Human genes 0.000 description 1
- 108010074328 Interferon-gamma Proteins 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- 241001550390 Leptospira interrogans serovar Canicola Species 0.000 description 1
- 108091036060 Linker DNA Proteins 0.000 description 1
- 208000016604 Lyme disease Diseases 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 208000030289 Lymphoproliferative disease Diseases 0.000 description 1
- 241000712079 Measles morbillivirus Species 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 241000948165 Milk vetch dwarf virus Species 0.000 description 1
- 241000711386 Mumps virus Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 101710187863 Myelomonocytic growth factor Proteins 0.000 description 1
- BACYUWVYYTXETD-UHFFFAOYSA-N N-Lauroylsarcosine Chemical compound CCCCCCCCCCCC(=O)N(C)CC(O)=O BACYUWVYYTXETD-UHFFFAOYSA-N 0.000 description 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
- 108091060545 Nonsense suppressor Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- 241000711504 Paramyxoviridae Species 0.000 description 1
- 208000002606 Paramyxoviridae Infections Diseases 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 208000005342 Porcine Reproductive and Respiratory Syndrome Diseases 0.000 description 1
- 101150093191 RIR1 gene Proteins 0.000 description 1
- 238000010802 RNA extraction kit Methods 0.000 description 1
- 206010037742 Rabies Diseases 0.000 description 1
- 241000711798 Rabies lyssavirus Species 0.000 description 1
- 208000004756 Respiratory Insufficiency Diseases 0.000 description 1
- 206010038678 Respiratory depression Diseases 0.000 description 1
- 102100037968 Ribonuclease inhibitor Human genes 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- 241000282695 Saimiri Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 241000555745 Sciuridae Species 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 241000725681 Swine influenza virus Species 0.000 description 1
- 241000700565 Swinepox virus Species 0.000 description 1
- 206010051379 Systemic Inflammatory Response Syndrome Diseases 0.000 description 1
- 208000000389 T-cell leukemia Diseases 0.000 description 1
- 208000028530 T-cell lymphoblastic leukemia/lymphoma Diseases 0.000 description 1
- 108700026226 TATA Box Proteins 0.000 description 1
- 108010055044 Tetanus Toxin Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 108010046722 Thrombospondin 1 Proteins 0.000 description 1
- 241001223089 Tremovirus A Species 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 101150099321 UL42 gene Proteins 0.000 description 1
- 101150048066 UL45 gene Proteins 0.000 description 1
- 241000700618 Vaccinia virus Species 0.000 description 1
- 241000700647 Variola virus Species 0.000 description 1
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 1
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 1
- 102100039037 Vascular endothelial growth factor A Human genes 0.000 description 1
- 241000711975 Vesicular stomatitis virus Species 0.000 description 1
- 230000010530 Virus Neutralization Effects 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000002788 anti-peptide Effects 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 239000003816 antisense DNA Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 241000701792 avian adenovirus Species 0.000 description 1
- 230000010310 bacterial transformation Effects 0.000 description 1
- 108010058966 bacteriophage T7 induced DNA polymerase Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 208000024753 bloody sputum Diseases 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 108010006025 bovine growth hormone Proteins 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 208000014058 canine distemper Diseases 0.000 description 1
- 210000000234 capsid Anatomy 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 230000016396 cytokine production Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- UFJPAQSLHAGEBL-RRKCRQDMSA-N dITP Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(N=CNC2=O)=C2N=C1 UFJPAQSLHAGEBL-RRKCRQDMSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
- 206010013023 diphtheria Diseases 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 241001493065 dsRNA viruses Species 0.000 description 1
- 201000002491 encephalomyelitis Diseases 0.000 description 1
- 108010030074 endodeoxyribonuclease MluI Proteins 0.000 description 1
- 210000003989 endothelium vascular Anatomy 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 108010052305 exodeoxyribonuclease III Proteins 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 230000037442 genomic alteration Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 101150029296 grifin gene Proteins 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 244000144980 herd Species 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000005745 host immune response Effects 0.000 description 1
- 230000004727 humoral immunity Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 230000000521 hyperimmunizing effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 229960003130 interferon gamma Drugs 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- YFVGRULMIQXYNE-UHFFFAOYSA-M lithium;dodecyl sulfate Chemical compound [Li+].CCCCCCCCCCCCOS([O-])(=O)=O YFVGRULMIQXYNE-UHFFFAOYSA-M 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 210000004237 neck muscle Anatomy 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 231100000862 numbness Toxicity 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 229940127240 opiate Drugs 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- LCCNCVORNKJIRZ-UHFFFAOYSA-N parathion Chemical compound CCOP(=S)(OCC)OC1=CC=C([N+]([O-])=O)C=C1 LCCNCVORNKJIRZ-UHFFFAOYSA-N 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 108010024226 placental ribonuclease inhibitor Proteins 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- HJRIWDYVYNNCFY-UHFFFAOYSA-M potassium;dimethylarsinate Chemical compound [K+].C[As](C)([O-])=O HJRIWDYVYNNCFY-UHFFFAOYSA-M 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 108010062697 pseudorabies virus glycoproteins Proteins 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 208000013220 shortness of breath Diseases 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000012153 swine disease Diseases 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229940118376 tetanus toxin Drugs 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 210000003437 trachea Anatomy 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 244000052613 viral pathogen Species 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 101150069452 z gene Proteins 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16311—Mardivirus, e.g. Gallid herpesvirus 2, Marek-like viruses, turkey HV
- C12N2710/16334—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16311—Mardivirus, e.g. Gallid herpesvirus 2, Marek-like viruses, turkey HV
- C12N2710/16341—Use of virus, viral particle or viral elements as a vector
- C12N2710/16343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/288,065 | 1994-08-09 | ||
| US08/288,065 US5961982A (en) | 1985-09-06 | 1994-08-09 | Recombinant herpesvirus of turkeys and uses thereof |
| US08/362,240 | 1994-12-22 | ||
| US08/362,240 US5965138A (en) | 1985-09-06 | 1994-12-22 | Recombinant chimeric virus and uses thereof |
| PCT/US1995/010245 WO1996005291A1 (en) | 1994-08-09 | 1995-08-09 | Recombinant herpesvirus of turkeys and uses thereof |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005274301A Division JP4339835B2 (ja) | 1994-08-09 | 2005-09-21 | 組換え型シチメンチョウ・ヘルペスウイルス、及びその使用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH10506782A JPH10506782A (ja) | 1998-07-07 |
| JP3756950B2 true JP3756950B2 (ja) | 2006-03-22 |
Family
ID=26964815
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP50753196A Expired - Fee Related JP3756950B2 (ja) | 1994-08-09 | 1995-08-09 | 組換え型シチメンチョウヘルペスウイルス、及びその使用 |
| JP2005274301A Expired - Lifetime JP4339835B2 (ja) | 1994-08-09 | 2005-09-21 | 組換え型シチメンチョウ・ヘルペスウイルス、及びその使用 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005274301A Expired - Lifetime JP4339835B2 (ja) | 1994-08-09 | 2005-09-21 | 組換え型シチメンチョウ・ヘルペスウイルス、及びその使用 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US5965138A (de) |
| EP (2) | EP1801204B1 (de) |
| JP (2) | JP3756950B2 (de) |
| AT (2) | ATE498006T1 (de) |
| AU (1) | AU711815C (de) |
| CA (1) | CA2196570C (de) |
| DE (2) | DE69536138D1 (de) |
| DK (1) | DK0776361T3 (de) |
| ES (1) | ES2278382T3 (de) |
| MX (1) | MX9700833A (de) |
| WO (1) | WO1996005291A1 (de) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0748631B1 (de) * | 1988-09-13 | 2006-02-08 | Mérial | Virale Vakzine |
| US6183753B1 (en) * | 1994-08-09 | 2001-02-06 | Schering-Plough Veterinary Corp. | Recombinant chimeric virus and uses thereof |
| US6913751B2 (en) * | 1992-06-12 | 2005-07-05 | Schering-Plough Veterinary Corporation | Recombinant avian herpesvirus useful in vaccine production |
| US6984728B2 (en) | 1993-09-24 | 2006-01-10 | Schering Corporation | Recombinant infectious laryngotracheitis virus and uses thereof |
| FR2728794B1 (fr) * | 1994-12-30 | 1997-03-21 | Rhone Merieux | Vaccin recombinant aviaire a base de virus herpes aviaire, notamment contre la maladie de gumboro |
| FR2728795B1 (fr) * | 1994-12-30 | 1997-03-21 | Rhone Merieux | Vaccin vivant recombinant aviaire, utilisant comme vecteur un virus herpes aviaire |
| DE69836557T2 (de) * | 1997-10-03 | 2007-09-20 | Zeon Corp. | Rekombinanten des infektiösen vogel-herpesvirus und rekombinante impfstoffe, die unter dessen verwendung hergestellt wurden |
| US6506385B1 (en) | 1998-04-17 | 2003-01-14 | Embrex, Inc. | Live vaccines and methods of treatment therewith |
| US6299882B1 (en) * | 1999-04-09 | 2001-10-09 | Schering Corporation | UL54.5 of Marek's disease virus (MDV) |
| JP2003528587A (ja) * | 1999-10-29 | 2003-09-30 | ヒューマン ジノーム サイエンシーズ， インコーポレイテッド | ２７個のヒト分泌タンパク質 |
| US6516246B2 (en) * | 2000-09-11 | 2003-02-04 | Mimicking Man Manually, Inc. | Method and system for determining native neurological dominant hemisphere |
| US7314715B2 (en) * | 2001-06-14 | 2008-01-01 | Schering-Plough Animal Health Corporation | Recombinant avian herpesvirus useful in vaccine production |
| US6764684B2 (en) | 2001-09-28 | 2004-07-20 | Zeon Corporation | Avian herpesvirus-based recombinant infectious bursal disease vaccine |
| US7205398B2 (en) | 2002-05-24 | 2007-04-17 | Schering-Plough Animal Health Corporation | Eta-1 gene and methods for use |
| US7897146B2 (en) | 2003-11-17 | 2011-03-01 | Crusade Laboratories Limited | Treatment using herpes simplex virus |
| GB0326798D0 (en) | 2003-11-17 | 2003-12-24 | Crusade Lab Ltd | Methods for generating mutant virus |
| JP2007511216A (ja) | 2003-11-17 | 2007-05-10 | クルセイド ラボラトリーズ リミテッド | 変異ウイルス |
| MX2007012383A (es) * | 2005-04-08 | 2007-11-07 | Bayer Bioscience Nv | Evento elite a2704-12 y metodos y equipos para identificar dicho evento en muestras biologicas. |
| US20080241188A1 (en) | 2007-03-30 | 2008-10-02 | Zeon Corporation | Turkey herpesvirus vectored recombinant containing avian influenza genes |
| CN102056941A (zh) * | 2008-06-23 | 2011-05-11 | 英特威国际有限公司 | 编码白细胞介素-12的重组火鸡疱疹病毒 |
| US9089360B2 (en) | 2008-08-06 | 2015-07-28 | Ethicon Endo-Surgery, Inc. | Devices and techniques for cutting and coagulating tissue |
| EP2768529A1 (de) | 2011-10-21 | 2014-08-27 | Intervet International B.V. | Rekombinanter nichtpathogener mdv-vektor für multivalente immunität |
| AR088401A1 (es) | 2011-10-21 | 2014-05-28 | Intervet Int Bv | Constructos del virus recombinante de la enfermedad de marek no patogenicos que codifican el virus de laringotraqueitis infecciosa y antigenos del virus de la enfermedad de newcastle |
| EP2644702A1 (de) | 2012-03-30 | 2013-10-02 | Ceva Sante Animale | Multivalentes rekombinantes Vogelherpesvirus und Impfstoff zur Immunisierung von Vogelarten |
| AR097029A1 (es) * | 2013-07-26 | 2016-02-17 | Intervet Int Bv | Aceleración de la respuesta inmune inducida por virus vectorial en aves, composición, uso, método para la vacunación y método para acelerar la respuesta inmune |
| AR103245A1 (es) | 2014-12-24 | 2017-04-26 | Intervet Int Bv | Vacuna vectorial basada en hvt (herpes virus de los pavos) frente a nd (enfermedad de newcastle) - ibd (enfermedad de gumboro) mejorada |
| BR112018076021A2 (pt) | 2016-06-17 | 2019-04-02 | Intervet International B.V. | constructos de vírus não patogênicos recombinantes da doença de marek que codificam os antígenos do vírus da laringotraqueíte infecciosa e do vírus da doença infecciosa bursal |
| PL3554536T3 (pl) | 2016-12-14 | 2023-03-06 | Boehringer Ingelheim Animal Health USA Inc. | Rekombinowane wektory hvt wyrażające wiele antygenów patogenów ptasich oraz szczepionki je zawierające |
| CN111542599A (zh) | 2017-10-12 | 2020-08-14 | 英特维特国际股份有限公司 | 编码多种异源抗原的重组非致病性马尔克氏病病毒构建体 |
| KR20220056241A (ko) | 2019-09-11 | 2022-05-04 | 조에티스 서비시즈 엘엘씨 | 조류 병원체의 항원을 발현하는 재조합 칠면조 헤르페스바이러스 벡터 및 이의 용도 |
| CN114828882A (zh) | 2019-12-20 | 2022-07-29 | 英特维特国际股份有限公司 | 多价hvt载体疫苗 |
| CA3186579A1 (en) | 2020-06-17 | 2021-12-23 | Boehringer Ingelheim Vetmedica Gmbh | Recombinant hvt vectors expressing influenza hemagglutinin and immunogenic compositions, and production and uses thereof |
| JP2024501943A (ja) | 2020-12-24 | 2024-01-17 | インターベット インターナショナル ベー． フェー． | 多価ｈｖｔベクターワクチン |
| WO2023213946A1 (en) | 2022-05-05 | 2023-11-09 | Intervet International B.V. | New multivalent hvt vector vaccine |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4859587A (en) * | 1984-06-04 | 1989-08-22 | Institut Merieux | Recombinant herpes simplex viruses, vaccines and methods |
| US5047237A (en) * | 1985-09-06 | 1991-09-10 | Prutech Research And Development Partnership | Attenuated pseudorabies virus having a deletion of at least a portion of a gene encoding an antigenic, nonessential protein, vaccine containing same and methods of identifying animals vaccinated with the vaccine |
| US5223424A (en) * | 1985-09-06 | 1993-06-29 | Prutech Research And Development | Attenuated herpesviruses and herpesviruses which include foreign DNA encoding an amino acid sequence |
| US5928648A (en) * | 1985-09-06 | 1999-07-27 | Syntro Corporation | Recombinant herpesvirus of turkeys and uses thereof |
| US4877737A (en) * | 1985-09-06 | 1989-10-31 | Prutech Research And Development Partnership | Attenuated pseudorabies virus which has a deletion in at least a portion of a repeat sequence and vaccine containing same |
| WO1988007088A1 (en) * | 1987-03-19 | 1988-09-22 | Synergen, Inc. | Viral vector system for the introduction of foreign dna expression products into gallinaceous birds |
| AU628294B2 (en) * | 1987-07-27 | 1992-09-17 | Syntro Corporation | Attenuated herpes viruses, herpes viruses which include foreign dna encoding an amino acid sequence and vaccines containing same |
| JP2583115B2 (ja) * | 1988-09-10 | 1997-02-19 | 財団法人化学及血清療法研究所 | 組換えマレック病ウイルスおよびその製法 |
| EP0748631B1 (de) * | 1988-09-13 | 2006-02-08 | Mérial | Virale Vakzine |
| US5225336A (en) * | 1989-03-08 | 1993-07-06 | Health Research Incorporated | Recombinant poxvirus host range selection system |
| ES2070997T3 (es) * | 1989-12-04 | 1995-06-16 | Akzo Nobel Nv | Virus de herpes recombinante de pavos y vacunas vector vivas derivadas de los mismos. |
| FR2659349B1 (fr) * | 1990-03-12 | 1993-12-24 | Rhone Merieux | Virus herpes recombinants notamment pour la realisation de vaccins, leur procede de preparation, les plasmides realises au cours de ce procede et les vaccins obtenus. |
| ES2056565T3 (es) * | 1990-07-30 | 1994-10-01 | Akzo Nobel Nv | Virus recombinante de la enfermedad de marek. |
| WO1992003554A1 (en) * | 1990-08-24 | 1992-03-05 | Arthur Webster Pty. Ltd. | Infectious laryngotracheitis virus vaccine |
| US5252717A (en) * | 1990-08-24 | 1993-10-12 | Board Of Trustees Operating Michigan State University | Marek's disease herpesvirus DNA segments encoding glycoproteins, gD, gI and gE |
| FR2666589B1 (fr) * | 1990-09-07 | 1994-08-05 | Rhone Merieux | Nouveaux virus herpes recombinants, vaccin a base de ces recombinants, leur procede de preparation, genes, vecteurs et plasmides utilises dans ce procede. |
| EP0486106A3 (en) * | 1990-11-16 | 1992-12-23 | Akzo N.V. | Marek's disease virus vaccine |
| US5240703A (en) * | 1991-03-01 | 1993-08-31 | Syntro Corporation | Attenuated, genetically-engineered pseudorabies virus s-prv-155 and uses thereof |
| JP3602530B2 (ja) * | 1991-03-07 | 2004-12-15 | ヴァイロジェネティクス コーポレイション | 遺伝子操作したワクチン菌株 |
| US5853733A (en) * | 1993-02-26 | 1998-12-29 | Syntro Corporation | Recombinant herpesvirus of turkeys and uses thereof |
| FR2697534A1 (fr) * | 1992-11-02 | 1994-05-06 | Rhone Merieux | Virus herpès de la dinde recombinant pour la réalisation de vaccin de la maladie de Gumboro, son procédé de préparation et vaccin obtenu. |
-
1994
- 1994-12-22 US US08/362,240 patent/US5965138A/en not_active Expired - Lifetime
-
1995
- 1995-08-09 WO PCT/US1995/010245 patent/WO1996005291A1/en active IP Right Grant
- 1995-08-09 EP EP06026820A patent/EP1801204B1/de not_active Expired - Lifetime
- 1995-08-09 EP EP95930814A patent/EP0776361B1/de not_active Expired - Lifetime
- 1995-08-09 DE DE69536138T patent/DE69536138D1/de not_active Expired - Lifetime
- 1995-08-09 ES ES95930814T patent/ES2278382T3/es not_active Expired - Lifetime
- 1995-08-09 AT AT06026820T patent/ATE498006T1/de not_active IP Right Cessation
- 1995-08-09 AT AT95930814T patent/ATE350467T1/de not_active IP Right Cessation
- 1995-08-09 AU AU34053/95A patent/AU711815C/en not_active Expired
- 1995-08-09 CA CA2196570A patent/CA2196570C/en not_active Expired - Lifetime
- 1995-08-09 JP JP50753196A patent/JP3756950B2/ja not_active Expired - Fee Related
- 1995-08-09 DK DK95930814T patent/DK0776361T3/da active
- 1995-08-09 MX MX9700833A patent/MX9700833A/es unknown
- 1995-08-09 DE DE69535359T patent/DE69535359T2/de not_active Expired - Lifetime
-
2005
- 2005-09-21 JP JP2005274301A patent/JP4339835B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DK0776361T3 (da) | 2007-04-30 |
| AU711815C (en) | 2003-02-20 |
| DE69535359T2 (de) | 2007-10-11 |
| WO1996005291A1 (en) | 1996-02-22 |
| EP1801204B1 (de) | 2011-02-09 |
| EP0776361B1 (de) | 2007-01-03 |
| JP2006055171A (ja) | 2006-03-02 |
| EP0776361A1 (de) | 1997-06-04 |
| JPH10506782A (ja) | 1998-07-07 |
| JP4339835B2 (ja) | 2009-10-07 |
| US5965138A (en) | 1999-10-12 |
| DE69535359D1 (de) | 2007-02-15 |
| ES2278382T3 (es) | 2007-08-01 |
| CA2196570A1 (en) | 1996-02-22 |
| MX9700833A (es) | 1997-06-28 |
| ATE498006T1 (de) | 2011-02-15 |
| EP1801204A1 (de) | 2007-06-27 |
| DE69536138D1 (de) | 2011-03-24 |
| AU711815B2 (en) | 1999-10-21 |
| EP0776361A4 (de) | 2003-06-04 |
| ATE350467T1 (de) | 2007-01-15 |
| AU3405395A (en) | 1996-03-07 |
| CA2196570C (en) | 2010-10-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3756950B2 (ja) | 組換え型シチメンチョウヘルペスウイルス、及びその使用 | |
| US5853733A (en) | Recombinant herpesvirus of turkeys and uses thereof | |
| EP0431668B1 (de) | Rekombinantes Truthahn-Herpesvirus und davon abgeleiteter Lebendimpfstoffvektor | |
| US6153199A (en) | Avian recombinant live vaccine using, as vector, the avian infectious laryngotracheitis virus | |
| US5928648A (en) | Recombinant herpesvirus of turkeys and uses thereof | |
| JP4440347B2 (ja) | 組換えキメラウイルスとその使用 | |
| KR100492283B1 (ko) | 재조합 돼지폭스 바이러스 | |
| US5961982A (en) | Recombinant herpesvirus of turkeys and uses thereof | |
| US6913751B2 (en) | Recombinant avian herpesvirus useful in vaccine production | |
| US5874279A (en) | Recombinant infectious bovine rhinotracheitis virus | |
| US20100008948A1 (en) | Recombinant herpesvirus useful in vaccine production | |
| AU729518B2 (en) | Recombinant fowlpox viruses and uses thereof | |
| JP3964458B2 (ja) | 組換え型伝染性喉頭気管炎ウイルスおよびその使用 | |
| US6410033B1 (en) | Recombinant infectious bovine rhinotracheitis virus | |
| US5783195A (en) | Recombinant infectious bovine rhinotracheitis virus S-IBR-052 and uses thereof | |
| ES2360298T3 (es) | Herpesvirus recombinantes de pavos y sus usos. | |
| AU750084B2 (en) | Recombinant herpesvirus of turkeys and uses thereof II | |
| AU684046C (en) | Recombinant herpesvirus of turkeys and uses thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20050322 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20050622 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20050808 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20050921 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A133 Effective date: 20051114 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20051114 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20051206 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20051226 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100106 Year of fee payment: 4 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313113 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100106 Year of fee payment: 4 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100106 Year of fee payment: 4 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110106 Year of fee payment: 5 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120106 Year of fee payment: 6 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130106 Year of fee payment: 7 |
|
| LAPS | Cancellation because of no payment of annual fees |