JP3343129B2 - タンパク質の結晶化 - Google Patents

タンパク質の結晶化

Info

Publication number
JP3343129B2
JP3343129B2 JP53358198A JP53358198A JP3343129B2 JP 3343129 B2 JP3343129 B2 JP 3343129B2 JP 53358198 A JP53358198 A JP 53358198A JP 53358198 A JP53358198 A JP 53358198A JP 3343129 B2 JP3343129 B2 JP 3343129B2
Authority
JP
Japan
Prior art keywords
acid
human insulin
insulin
buffer
nεb29
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP53358198A
Other languages
English (en)
Japanese (ja)
Other versions
JP2000510161A (ja
Inventor
ホワイッティングハム、ジーン・レスリー
バルシュミット、パー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of JP2000510161A publication Critical patent/JP2000510161A/ja
Application granted granted Critical
Publication of JP3343129B2 publication Critical patent/JP3343129B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP53358198A 1997-02-07 1998-02-03 タンパク質の結晶化 Expired - Fee Related JP3343129B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK0146/97 1997-02-07
DK14697 1997-02-07
PCT/DK1998/000042 WO1998034953A1 (en) 1997-02-07 1998-02-03 Crystallisation of proteins

Publications (2)

Publication Number Publication Date
JP2000510161A JP2000510161A (ja) 2000-08-08
JP3343129B2 true JP3343129B2 (ja) 2002-11-11

Family

ID=8090294

Family Applications (1)

Application Number Title Priority Date Filing Date
JP53358198A Expired - Fee Related JP3343129B2 (ja) 1997-02-07 1998-02-03 タンパク質の結晶化

Country Status (7)

Country Link
EP (1) EP0966482B1 (de)
JP (1) JP3343129B2 (de)
AT (1) ATE294189T1 (de)
AU (1) AU5749198A (de)
DE (1) DE69829953T2 (de)
ES (1) ES2242270T3 (de)
WO (1) WO1998034953A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA200000453A1 (ru) 1997-10-24 2000-10-30 Эли Лилли Энд Компани Композиции нерастворимого инсулина
US6531448B1 (en) 1997-12-23 2003-03-11 Eli Lilly And Company Insoluble compositions for controlling blood glucose
ES2180511T3 (es) * 1999-01-26 2003-02-16 Lilly Co Eli Formulaciones monodispersas de analogos de insulina acilados hexamericos.
JP5484041B2 (ja) * 2007-02-23 2014-05-07 学校法人関西学院 蛋白質結晶化剤および蛋白質結晶化剤を用いた蛋白質結晶化方法
EP2464655B1 (de) * 2009-08-11 2017-02-15 Biocon Limited Chromatographieverfahren
AU2012213432B2 (en) 2011-02-01 2016-10-13 Novo Nordisk A/S Purification of insulin
WO2016032869A1 (en) 2014-08-26 2016-03-03 Merck Sharp & Dohme Corp. Method for preparing crystalline insulin or insulin analog compositions

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3856771A (en) * 1973-07-16 1974-12-24 Lilly Co Eli Process for manufacturing alkali metal or ammonium insulin
DE3327709A1 (de) * 1983-07-29 1985-02-07 Hoechst Ag, 6230 Frankfurt Insulin-derivat-kristallsuspensionen, verfahren zu deren herstellung und deren verwendung
US5461031A (en) * 1994-06-16 1995-10-24 Eli Lilly And Company Monomeric insulin analog formulations
US5700904A (en) * 1995-06-07 1997-12-23 Eli Lilly And Company Preparation of an acylated protein powder

Also Published As

Publication number Publication date
EP0966482B1 (de) 2005-04-27
ES2242270T3 (es) 2005-11-01
DE69829953D1 (de) 2005-06-02
ATE294189T1 (de) 2005-05-15
EP0966482A1 (de) 1999-12-29
DE69829953T2 (de) 2006-03-02
AU5749198A (en) 1998-08-26
JP2000510161A (ja) 2000-08-08
WO1998034953A1 (en) 1998-08-13

Similar Documents

Publication Publication Date Title
US5898067A (en) Crystallization of proteins
JP3844504B2 (ja) アシル化タンパク質粉末の製造
JP3962430B2 (ja) 脂肪酸アシル化蛋白質のゲル化削減法
EP0254516B1 (de) Peptide
US4959351A (en) Crystal suspensions of insulin derivatives, processes for their preparation and their use
JP2002508332A (ja) グルカゴン様ペプチド−１結晶
US5830999A (en) Stabilization of insulin through ligand binding interations
JPS6042334A (ja) 糖尿病治療用薬剤
JPS61212598A (ja) インシユリン化合物
JPS6042397A (ja) 新規なインシユリン誘導体およびそれらの製造法
JPH02218696A (ja) 新規インシュリン誘導体、それらの使用およびそれらを含む医薬組成物
JP2000504732A (ja) インスリン誘導体類とその使用
JP2003523366A (ja) グルカゴン様ペプチド１化合物の可溶化方法
JPH11502856A (ja) 単量体インスリン類似体製剤
EP0140084A1 (de) Verfahren zur Herstellung von Insulin-Derivaten, deren B-Kette C-terminal verlängert ist, neue basisch modifizierte Insulin-Derivate, diese enthaltende Mittel und ihre Verwendung
JP3131215B2 (ja) 新規インスリン誘導体
JP3343129B2 (ja) タンパク質の結晶化
US5597893A (en) Preparation of stable insulin analog crystals
US5466666A (en) Amorphous monospheric forms of insulin derivatives
MXPA97009657A (en) Preparation of an acil protein dust
HK1014010A (en) Reducing gelation of a fatty acid acylated protein using a citrate buffer
JPS6072857A (ja) 高純度ｓ−カルボキシメチル−ｌ−システイン塩酸塩の製造法

Legal Events

Date Code Title Description
R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20080823

Year of fee payment: 6

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20080823

Year of fee payment: 6

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20090823

Year of fee payment: 7

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20090823

Year of fee payment: 7

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20100823

Year of fee payment: 8

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110823

Year of fee payment: 9

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110823

Year of fee payment: 9

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120823

Year of fee payment: 10

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130823

Year of fee payment: 11

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees