JP3108105B2 - 共沈剤及び核酸の抽出方法 - Google Patents
共沈剤及び核酸の抽出方法Info
- Publication number
- JP3108105B2 JP3108105B2 JP09505013A JP50501397A JP3108105B2 JP 3108105 B2 JP3108105 B2 JP 3108105B2 JP 09505013 A JP09505013 A JP 09505013A JP 50501397 A JP50501397 A JP 50501397A JP 3108105 B2 JP3108105 B2 JP 3108105B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- coprecipitant
- extracting
- alcohol
- biological material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- HXJUTPCZVOIRIF-UHFFFAOYSA-N sulfolane Chemical compound O=S1(=O)CCCC1 HXJUTPCZVOIRIF-UHFFFAOYSA-N 0.000 description 1
- 150000003464 sulfur compounds Chemical class 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- DQWPFSLDHJDLRL-UHFFFAOYSA-N triethyl phosphate Chemical compound CCOP(=O)(OCC)OCC DQWPFSLDHJDLRL-UHFFFAOYSA-N 0.000 description 1
- SZYJELPVAFJOGJ-UHFFFAOYSA-N trimethylamine hydrochloride Chemical compound Cl.CN(C)C SZYJELPVAFJOGJ-UHFFFAOYSA-N 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 229940100445 wheat starch Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21186295 | 1995-08-21 | ||
| JP7-211862 | 1995-08-21 | ||
| PCT/JP1996/002263 WO1997007207A1 (fr) | 1995-08-21 | 1996-08-09 | Co-precipitant et procede d'extraction d'acides nucleiques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP3108105B2 true JP3108105B2 (ja) | 2000-11-13 |
Family
ID=16612845
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP09505013A Expired - Lifetime JP3108105B2 (ja) | 1995-08-21 | 1996-08-09 | 共沈剤及び核酸の抽出方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6815541B1 (en15) |
| EP (1) | EP0792932B1 (en15) |
| JP (1) | JP3108105B2 (en15) |
| DE (1) | DE69632904T2 (en15) |
| WO (1) | WO1997007207A1 (en15) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE263254T1 (de) * | 1995-10-02 | 2004-04-15 | Emd Biosciences Inc | Verfahren zur fällung von nukleinsäuren mit einem sichtbaren träger |
| US7144713B1 (en) | 1995-10-02 | 2006-12-05 | Emd Biosciences, Inc. | Method for precipitating nucleic acid with visible carrier |
| US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
| US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
| US7081366B2 (en) * | 2003-06-02 | 2006-07-25 | Northrop Grumman Corporation | Uniform bead dosing from a stable dispersion |
| US8686129B2 (en) * | 2007-03-20 | 2014-04-01 | Agilent Technologies, Inc. | Methods for the separation of biological molecules using sulfolane |
| JP5743701B2 (ja) * | 2011-05-10 | 2015-07-01 | 英二 小西 | Ｒｎａ検出用試薬およびｒｎａ検出方法 |
| JP5743704B2 (ja) * | 2011-05-12 | 2015-07-01 | 住友大阪セメント株式会社 | Ｒｎａ検出用試薬およびｒｎａ検出方法 |
| MX347611B (es) | 2011-06-19 | 2017-05-04 | Abogen Inc | Dispositivos, soluciones y metodos para recoleccion de muestras. |
| CN103571824B (zh) | 2012-08-09 | 2016-04-13 | 财团法人工业技术研究院 | 用于分离核酸的组合物及方法 |
| IN2015DN02893A (en15) | 2012-10-04 | 2015-09-11 | Univ Leland Stanford Junior | |
| JP6733554B2 (ja) * | 2015-01-21 | 2020-08-05 | 味の素株式会社 | 沈殿促進剤およびそれを用いる沈殿化方法 |
| CN109477098A (zh) * | 2016-05-31 | 2019-03-15 | Dna真诺泰克有限公司 | 用于从水溶液去除去垢剂的组合物、系统和方法 |
| CN111254138A (zh) * | 2018-12-03 | 2020-06-09 | 北京银丰鼎诚生物工程技术有限公司 | 一种分子克隆中提高酶切产物浓缩效率的方法 |
| WO2025085682A1 (en) | 2023-10-18 | 2025-04-24 | Amgen Inc. | Evaluation of mrna transcript levels using ddpcr for early pool and single-cell clone assessment in cell line development |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2588383B1 (fr) | 1985-10-04 | 1988-01-08 | Inst Nat Sante Rech Med | Nouveau procede de recuperation et de dosage de microquantites d'acide desoxyribonucleique dans un liquide biologique acellulaire |
| EP0338591A3 (en) | 1988-04-21 | 1991-09-04 | Microprobe Corporation | Nucleic acid extraction method |
| ATE172466T1 (de) | 1990-07-13 | 1998-11-15 | Microprobe Corp | Nicht-ätzende zusammensetzung und verfahren zur gewinnung von nukleinsäuren |
| JP2548494B2 (ja) | 1992-07-27 | 1996-10-30 | 三光純薬株式会社 | 核酸抽出方法 |
| WO1995002050A1 (en) | 1993-07-08 | 1995-01-19 | Tepnel Medical Limited | Obtaining nucleic acids |
| JP3451667B2 (ja) * | 1993-08-24 | 2003-09-29 | 東ソー株式会社 | 核酸抽出及び特定核酸配列の検出方法 |
-
1996
- 1996-08-09 JP JP09505013A patent/JP3108105B2/ja not_active Expired - Lifetime
- 1996-08-09 US US08/817,101 patent/US6815541B1/en not_active Expired - Fee Related
- 1996-08-09 DE DE1996632904 patent/DE69632904T2/de not_active Expired - Fee Related
- 1996-08-09 EP EP96926615A patent/EP0792932B1/en not_active Expired - Lifetime
- 1996-08-09 WO PCT/JP1996/002263 patent/WO1997007207A1/ja active IP Right Grant
Non-Patent Citations (2)
| Title |
|---|
| 今堀和友ら監修「生化学事典（第１版）」（１９８４−４−１０）株式会社東京化学同人ｐ．２０ |
| 日本生化学会編「新生化学実験講座２ 核酸▲Ｉ▼−分離精製−」（１９９１−７−１０）株式会社東京化学同人ｐ．１５ |
Also Published As
| Publication number | Publication date |
|---|---|
| DE69632904T2 (de) | 2004-11-18 |
| DE69632904D1 (de) | 2004-08-19 |
| US6815541B1 (en) | 2004-11-09 |
| EP0792932A4 (en) | 1999-10-20 |
| WO1997007207A1 (fr) | 1997-02-27 |
| EP0792932B1 (en) | 2004-07-14 |
| EP0792932A1 (en) | 1997-09-03 |
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