JP2955345B2 - リムラス変形細胞溶解産物および発色原基質を用いたエンドトキシン反応速度論的アッセイ - Google Patents
リムラス変形細胞溶解産物および発色原基質を用いたエンドトキシン反応速度論的アッセイInfo
- Publication number
- JP2955345B2 JP2955345B2 JP2295164A JP29516490A JP2955345B2 JP 2955345 B2 JP2955345 B2 JP 2955345B2 JP 2295164 A JP2295164 A JP 2295164A JP 29516490 A JP29516490 A JP 29516490A JP 2955345 B2 JP2955345 B2 JP 2955345B2
- Authority
- JP
- Japan
- Prior art keywords
- endotoxin
- assay
- sample
- kinetic
- substrate composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 238000003367 kinetic assay Methods 0.000 title claims abstract description 33
- 239000003593 chromogenic compound Substances 0.000 title claims abstract description 32
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- 108010072542 endotoxin binding proteins Proteins 0.000 title claims description 11
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US42830089A | 1989-10-30 | 1989-10-30 | |
| US428300 | 2009-04-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03220456A JPH03220456A (ja) | 1991-09-27 |
| JP2955345B2 true JP2955345B2 (ja) | 1999-10-04 |
Family
ID=23698313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2295164A Expired - Lifetime JP2955345B2 (ja) | 1989-10-30 | 1990-10-30 | リムラス変形細胞溶解産物および発色原基質を用いたエンドトキシン反応速度論的アッセイ |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5310657A (enExample) |
| EP (1) | EP0426395B1 (enExample) |
| JP (1) | JP2955345B2 (enExample) |
| AT (1) | ATE148230T1 (enExample) |
| AU (1) | AU644109B2 (enExample) |
| CA (1) | CA2028789C (enExample) |
| DE (1) | DE69029785T2 (enExample) |
| DK (1) | DK0426395T3 (enExample) |
| ES (1) | ES2098256T3 (enExample) |
| GR (1) | GR3023298T3 (enExample) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0549102B1 (en) * | 1991-12-25 | 1996-07-03 | Maruha Corporation | Beta-glucans detection reagents and methods of detecting beta-glucans |
| JP3242733B2 (ja) * | 1993-02-26 | 2001-12-25 | 生化学工業株式会社 | エンドトキシン特異的測定剤 |
| US6270982B1 (en) | 1997-10-09 | 2001-08-07 | Charles River Laboratories | Methods and compositions for the detection of bacterial endotoxins |
| JP4235279B2 (ja) * | 1998-04-10 | 2009-03-11 | 生化学工業株式会社 | 新規酵素反応測定法 |
| AU2003225254A1 (en) * | 2002-04-30 | 2003-11-17 | Biowhittaker Technologies, Inc. | Automated sequential injection analysis systems for the determination of trace endotoxin levels |
| ATE427068T1 (de) * | 2002-12-20 | 2009-04-15 | Charlotte Mecklenburg Hospital | Wegwerf-handvorrichtung zum auffangen von ausgeatmetem atemkondensat |
| US7828741B2 (en) * | 2002-12-20 | 2010-11-09 | The Charlotte-Mecklenburg Hospital Authority | Utilizing lipopolysaccharide in exhaled breath condensate to diagnose gram negative pneumonia |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5415797A (en) * | 1977-06-14 | 1979-02-05 | Seikagaku Kogyo Co Ltd | Detection and measurement of toxin in cells |
| US4322217A (en) * | 1980-06-27 | 1982-03-30 | Mallinckrodt, Inc. | Process for preparing Limulus lysate |
| US4406832A (en) * | 1981-09-03 | 1983-09-27 | Mallinckrodt, Inc. | Peptide-type substrates useful in the quantitative determination of endotoxin |
| US4510241A (en) * | 1981-09-03 | 1985-04-09 | Mallinckrodt, Inc. | Peptide-type substrates useful in the quantitative determination of endotoxin |
| DE3439761A1 (de) * | 1984-10-31 | 1986-05-07 | Klaus-Peter 6800 Mannheim Becker | Verfahren zur bestimmung von endotoxinkonzentrationen |
-
1990
- 1990-10-26 DK DK90311771.1T patent/DK0426395T3/da active
- 1990-10-26 ES ES90311771T patent/ES2098256T3/es not_active Expired - Lifetime
- 1990-10-26 AT AT90311771T patent/ATE148230T1/de not_active IP Right Cessation
- 1990-10-26 EP EP90311771A patent/EP0426395B1/en not_active Expired - Lifetime
- 1990-10-26 DE DE69029785T patent/DE69029785T2/de not_active Expired - Lifetime
- 1990-10-29 AU AU65556/90A patent/AU644109B2/en not_active Expired
- 1990-10-29 CA CA002028789A patent/CA2028789C/en not_active Expired - Lifetime
- 1990-10-30 JP JP2295164A patent/JP2955345B2/ja not_active Expired - Lifetime
-
1992
- 1992-07-13 US US07/911,375 patent/US5310657A/en not_active Expired - Lifetime
-
1997
- 1997-04-08 GR GR970400118T patent/GR3023298T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US5310657A (en) | 1994-05-10 |
| ES2098256T3 (es) | 1997-05-01 |
| GR3023298T3 (en) | 1997-07-30 |
| JPH03220456A (ja) | 1991-09-27 |
| CA2028789A1 (en) | 1991-05-01 |
| EP0426395A1 (en) | 1991-05-08 |
| DE69029785T2 (de) | 1997-05-28 |
| AU6555690A (en) | 1991-05-02 |
| DK0426395T3 (enExample) | 1997-03-03 |
| DE69029785D1 (de) | 1997-03-06 |
| CA2028789C (en) | 2001-02-06 |
| AU644109B2 (en) | 1993-12-02 |
| ATE148230T1 (de) | 1997-02-15 |
| EP0426395B1 (en) | 1997-01-22 |
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