ES2098256T3 - Ensayo cinetico para la endotoxina utilizando lisado amebocitico de limulus y sustrato cromogenico. - Google Patents
Ensayo cinetico para la endotoxina utilizando lisado amebocitico de limulus y sustrato cromogenico.Info
- Publication number
- ES2098256T3 ES2098256T3 ES90311771T ES90311771T ES2098256T3 ES 2098256 T3 ES2098256 T3 ES 2098256T3 ES 90311771 T ES90311771 T ES 90311771T ES 90311771 T ES90311771 T ES 90311771T ES 2098256 T3 ES2098256 T3 ES 2098256T3
- Authority
- ES
- Spain
- Prior art keywords
- endotoxin
- chromogenic substrate
- limulus
- amebotic
- list
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
SE PROVEE UN PROCEDIMIENTO DE ENSAYO CINETICO QUE PERMITE LA MEDICION DE LA CONCENTRACION DE ENDOTOXINAS EN UNA AMPLITUD DE DESDE 0.005 EU/ML A 50 EU/ML. EL PROCEDIMIENTO DE ENSAYO CUENTA CON UNA COMPOSICION DE UN SOLO SUBSTRATO REAGENTE QUE CONTIENE LISATO DE AMEBOCITA LIMULO Y UN SUBSTRATO CROMOGENICO. ESTE PROCEDIMIENTO DE ENSAYO ES FACILMENTE AUTOMATIZADO Y SUMINISTRA UNA SUBSTANCIAL MEJORA EN AMPLITUD Y SENSIBILIDAD SOBRE ENSAYOS DE ENDOTOXINAS ANTES DISPONIBLES.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US42830089A | 1989-10-30 | 1989-10-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2098256T3 true ES2098256T3 (es) | 1997-05-01 |
Family
ID=23698313
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES90311771T Expired - Lifetime ES2098256T3 (es) | 1989-10-30 | 1990-10-26 | Ensayo cinetico para la endotoxina utilizando lisado amebocitico de limulus y sustrato cromogenico. |
Country Status (10)
Country | Link |
---|---|
US (1) | US5310657A (es) |
EP (1) | EP0426395B1 (es) |
JP (1) | JP2955345B2 (es) |
AT (1) | ATE148230T1 (es) |
AU (1) | AU644109B2 (es) |
CA (1) | CA2028789C (es) |
DE (1) | DE69029785T2 (es) |
DK (1) | DK0426395T3 (es) |
ES (1) | ES2098256T3 (es) |
GR (1) | GR3023298T3 (es) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69211973T2 (de) * | 1991-12-25 | 1996-12-19 | Maruha Corp | Reagenzien und Verfahren zur Bestimmung von Beta-Glucanen |
JP3242733B2 (ja) * | 1993-02-26 | 2001-12-25 | 生化学工業株式会社 | エンドトキシン特異的測定剤 |
US6270982B1 (en) | 1997-10-09 | 2001-08-07 | Charles River Laboratories | Methods and compositions for the detection of bacterial endotoxins |
JP4235279B2 (ja) * | 1998-04-10 | 2009-03-11 | 生化学工業株式会社 | 新規酵素反応測定法 |
AU2003225254A1 (en) * | 2002-04-30 | 2003-11-17 | Biowhittaker Technologies, Inc. | Automated sequential injection analysis systems for the determination of trace endotoxin levels |
ATE427068T1 (de) * | 2002-12-20 | 2009-04-15 | Charlotte Mecklenburg Hospital | Wegwerf-handvorrichtung zum auffangen von ausgeatmetem atemkondensat |
US7828741B2 (en) * | 2002-12-20 | 2010-11-09 | The Charlotte-Mecklenburg Hospital Authority | Utilizing lipopolysaccharide in exhaled breath condensate to diagnose gram negative pneumonia |
US7547285B2 (en) * | 2003-02-14 | 2009-06-16 | The Charlotte-Mecklenburg Hospital Authority | Device and method for collection of exhaled alveolar breath condensate |
US7329538B2 (en) | 2003-03-17 | 2008-02-12 | Charles River Laboratories, Inc. | Methods and compositions for the detection of microbial contaminants |
US20050048655A1 (en) * | 2003-04-18 | 2005-03-03 | Novitsky Thomas J. | Kit for detecting endotoxin |
EP1627040A2 (en) * | 2003-04-18 | 2006-02-22 | Associates Of Cape Cod, Inc. | Kit for detecting endotoxin in aqueous solutions |
US20050124013A1 (en) * | 2003-11-07 | 2005-06-09 | Cambrex Bio Science Walkersville, Inc. | On-line apparatus and method for determining endotoxin levels |
WO2007078268A2 (en) | 2004-12-02 | 2007-07-12 | Charles River Laboratories, Inc. | Methods and compositions for the detection and/or quantification of gram positive bacterial contaminants |
ATE419526T1 (de) | 2005-01-13 | 2009-01-15 | Charles River Lab Inc | Verfahren zur klassifizierung von mikroben in einer biologischen probe |
US20090208995A1 (en) * | 2006-07-07 | 2009-08-20 | Seikagaku Corporation | Pro-clotting enzyme, and method for detection of endotoxin or (1-3)-beta-d-glucan using the same |
US20080124248A1 (en) * | 2006-11-29 | 2008-05-29 | Edward Andrew Sobek | Endotoxins and glucans quantification in indoor air |
US20090286692A1 (en) * | 2008-04-15 | 2009-11-19 | Wainwright Norman R | Cartridge and Method for Sample Analysis |
JP5489680B2 (ja) * | 2009-12-02 | 2014-05-14 | 興和株式会社 | 生物由来の生理活性物質の測定方法、それを実行するためのプログラム及び、生物由来の生理活性物質の測定装置 |
KR20120001755A (ko) | 2009-03-13 | 2012-01-04 | 코와 가부시키가이샤 | 생물 유래의 생리 활성 물질의 측정 방법, 그것을 실행하기 위한 프로그램, 및 생물 유래의 생리 활성 물질의 측정 장치 |
WO2010151751A2 (en) | 2009-06-26 | 2010-12-29 | Charles River Laboratories, Inc. | Heat-treated limulus amebocyte lysates |
GB2477752A (en) * | 2010-02-11 | 2011-08-17 | Arab Biotechnology Company | Detection of bacteria |
CU24073B1 (es) | 2011-12-27 | 2015-01-29 | Ct De Investigación Y Desarrollo De Medicamentos Cidem | COMPOSICIÓN A PARTIR DE EXTRACTO DE HEMOCITOS DE LANGOSTA PARA LA DETECCIÓN DE LIPOPOLISACÁRIDOS, PEPTIDOGLICANOS Y 1,3-ß-D-GLUCANOS |
US20130078665A1 (en) * | 2012-07-18 | 2013-03-28 | Deepika Bodapati | Test strip, a test kit and a method for detection of endotoxin in food |
WO2014058757A2 (en) | 2012-10-08 | 2014-04-17 | General Electric Company | Centripetal microfluidic platform for lal-reactive substances testing |
US10309947B2 (en) | 2013-02-27 | 2019-06-04 | Trace-Ability, Inc. | System and method for radiosynthesis, quality control and dose dispensing |
US10895563B2 (en) | 2014-02-26 | 2021-01-19 | Trace-Ability, Inc. | Palette-based systems for analyte characterization |
US11002717B2 (en) | 2014-02-26 | 2021-05-11 | Trace-Ability, Inc. | Systems and methods for characterizing radioactive analytes |
KR102561368B1 (ko) * | 2016-08-03 | 2023-08-01 | 론자 워커스빌 아이엔씨. | 실질적으로 코아굴로겐이 없는 리물루스 아메보사이트 라이세이트를 이용한 내독소 검출방법 |
WO2018132562A1 (en) | 2017-01-11 | 2018-07-19 | Lonza Walkersville, Inc. | Coagulogen-free clarified limulus amebocyte lysate |
KR20200019739A (ko) | 2017-06-28 | 2020-02-24 | 론자 워커스빌 아이엔씨. | 내독소 검출용 카트리지 |
US11892397B2 (en) * | 2017-10-17 | 2024-02-06 | Board Of Regents, The University Of Texas System | Endotoxin testing assay and method of same |
CN109387637A (zh) * | 2018-10-12 | 2019-02-26 | 四川升和药业股份有限公司 | 丹参注射液细菌内毒素检测方法 |
CN111077307B (zh) * | 2018-10-21 | 2022-08-23 | 厦门鲎试剂生物科技股份有限公司 | 一种用革兰氏阴性细菌感染快速检测脓毒病的新方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5415797A (en) * | 1977-06-14 | 1979-02-05 | Seikagaku Kogyo Co Ltd | Detection and measurement of toxin in cells |
US4322217A (en) * | 1980-06-27 | 1982-03-30 | Mallinckrodt, Inc. | Process for preparing Limulus lysate |
US4406832A (en) * | 1981-09-03 | 1983-09-27 | Mallinckrodt, Inc. | Peptide-type substrates useful in the quantitative determination of endotoxin |
US4510241A (en) * | 1981-09-03 | 1985-04-09 | Mallinckrodt, Inc. | Peptide-type substrates useful in the quantitative determination of endotoxin |
DE3439761A1 (de) * | 1984-10-31 | 1986-05-07 | Klaus-Peter 6800 Mannheim Becker | Verfahren zur bestimmung von endotoxinkonzentrationen |
-
1990
- 1990-10-26 ES ES90311771T patent/ES2098256T3/es not_active Expired - Lifetime
- 1990-10-26 AT AT90311771T patent/ATE148230T1/de not_active IP Right Cessation
- 1990-10-26 DE DE69029785T patent/DE69029785T2/de not_active Expired - Lifetime
- 1990-10-26 EP EP90311771A patent/EP0426395B1/en not_active Expired - Lifetime
- 1990-10-26 DK DK90311771.1T patent/DK0426395T3/da active
- 1990-10-29 AU AU65556/90A patent/AU644109B2/en not_active Expired
- 1990-10-29 CA CA002028789A patent/CA2028789C/en not_active Expired - Lifetime
- 1990-10-30 JP JP2295164A patent/JP2955345B2/ja not_active Expired - Lifetime
-
1992
- 1992-07-13 US US07/911,375 patent/US5310657A/en not_active Expired - Lifetime
-
1997
- 1997-04-08 GR GR970400118T patent/GR3023298T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
ATE148230T1 (de) | 1997-02-15 |
JPH03220456A (ja) | 1991-09-27 |
EP0426395B1 (en) | 1997-01-22 |
AU6555690A (en) | 1991-05-02 |
JP2955345B2 (ja) | 1999-10-04 |
EP0426395A1 (en) | 1991-05-08 |
DE69029785D1 (de) | 1997-03-06 |
DE69029785T2 (de) | 1997-05-28 |
AU644109B2 (en) | 1993-12-02 |
CA2028789C (en) | 2001-02-06 |
GR3023298T3 (en) | 1997-07-30 |
US5310657A (en) | 1994-05-10 |
CA2028789A1 (en) | 1991-05-01 |
DK0426395T3 (es) | 1997-03-03 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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