JP2025121906A5 - - Google Patents
Info
- Publication number
- JP2025121906A5 JP2025121906A5 JP2025065457A JP2025065457A JP2025121906A5 JP 2025121906 A5 JP2025121906 A5 JP 2025121906A5 JP 2025065457 A JP2025065457 A JP 2025065457A JP 2025065457 A JP2025065457 A JP 2025065457A JP 2025121906 A5 JP2025121906 A5 JP 2025121906A5
- Authority
- JP
- Japan
- Prior art keywords
- effector protein
- sequence
- cas9 effector
- guide
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762587029P | 2017-11-16 | 2017-11-16 | |
| US62/587,029 | 2017-11-16 | ||
| US201862693690P | 2018-07-03 | 2018-07-03 | |
| US62/693,690 | 2018-07-03 | ||
| PCT/US2018/061680 WO2019099943A1 (en) | 2017-11-16 | 2018-11-16 | Compositions and methods for improving the efficacy of cas9-based knock-in strategies |
| JP2020526198A JP7423520B2 (ja) | 2017-11-16 | 2018-11-16 | Ｃａｓ９ベースノックイン方針の効力を改善するための組成物及び方法 |
| JP2024005210A JP7703707B2 (ja) | 2017-11-16 | 2024-01-17 | Ｃａｓ９ベースノックイン方針の効力を改善するための組成物及び方法 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024005210A Division JP7703707B2 (ja) | 2017-11-16 | 2024-01-17 | Ｃａｓ９ベースノックイン方針の効力を改善するための組成物及び方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2025121906A JP2025121906A (ja) | 2025-08-20 |
| JP2025121906A5 true JP2025121906A5 (https=) | 2025-08-29 |
Family
ID=64870555
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020526198A Active JP7423520B2 (ja) | 2017-11-16 | 2018-11-16 | Ｃａｓ９ベースノックイン方針の効力を改善するための組成物及び方法 |
| JP2024005210A Active JP7703707B2 (ja) | 2017-11-16 | 2024-01-17 | Ｃａｓ９ベースノックイン方針の効力を改善するための組成物及び方法 |
| JP2025065457A Pending JP2025121906A (ja) | 2017-11-16 | 2025-04-11 | Ｃａｓ９ベースノックイン方針の効力を改善するための組成物及び方法 |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020526198A Active JP7423520B2 (ja) | 2017-11-16 | 2018-11-16 | Ｃａｓ９ベースノックイン方針の効力を改善するための組成物及び方法 |
| JP2024005210A Active JP7703707B2 (ja) | 2017-11-16 | 2024-01-17 | Ｃａｓ９ベースノックイン方針の効力を改善するための組成物及び方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US20210180059A1 (https=) |
| EP (1) | EP3710583A1 (https=) |
| JP (3) | JP7423520B2 (https=) |
| CN (2) | CN120310773A (https=) |
| WO (1) | WO2019099943A1 (https=) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9637739B2 (en) * | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| EP3555297A1 (en) | 2016-12-19 | 2019-10-23 | Editas Medicine, Inc. | Assessing nuclease cleavage |
| JP7229923B2 (ja) | 2017-01-06 | 2023-02-28 | エディタス・メディシン、インコーポレイテッド | ヌクレアーゼ切断を評価する方法 |
| US11499151B2 (en) | 2017-04-28 | 2022-11-15 | Editas Medicine, Inc. | Methods and systems for analyzing guide RNA molecules |
| MX2019014640A (es) | 2017-06-09 | 2020-10-05 | Editas Medicine Inc | Nucleasas de repeticiones palíndromicas agrupadas y regularmente interespaciadas de proteina asociada 9 (cas9) genomanipuladas. |
| US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| WO2019032628A1 (en) | 2017-08-07 | 2019-02-14 | The Regents Of The University Of California | GENERATING PLATFORM FOR SAFE CELLULAR THERAPEUTIC AGENTS |
| KR20210045360A (ko) | 2018-05-16 | 2021-04-26 | 신테고 코포레이션 | 가이드 rna 설계 및 사용을 위한 방법 및 시스템 |
| EP3823633A4 (en) | 2018-06-29 | 2023-05-03 | Editas Medicine, Inc. | SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO |
| US11530425B2 (en) | 2019-10-09 | 2022-12-20 | Massachusetts Institute Of Technology | Systems, methods, and compositions for correction of frameshift mutations |
| EP4133069A2 (en) | 2020-04-08 | 2023-02-15 | Astrazeneca AB | Compositions and methods for improved site-specific modification |
| CN116209751A (zh) * | 2020-07-07 | 2023-06-02 | 北京奥源和力生物技术有限公司 | 用于hsv-1基因编辑的指导rna及其方法 |
| CN114438235A (zh) * | 2020-11-04 | 2022-05-06 | 深圳市南山区疾病预防控制中心 | 一种同时检测嗜低温弓形杆菌及斯氏弓形杆菌的引物探针组件、试剂盒及其应用 |
| WO2022214522A2 (en) | 2021-04-07 | 2022-10-13 | Astrazeneca Ab | Compositions and methods for site-specific modification |
| US20240384246A1 (en) | 2021-05-27 | 2024-11-21 | Astrazeneca Ab | Ca s9 effector proteins with enhanced stability |
| CN113481184A (zh) * | 2021-08-06 | 2021-10-08 | 北京大学 | 融合蛋白以及其使用方法 |
| WO2023052508A2 (en) | 2021-09-30 | 2023-04-06 | Astrazeneca Ab | Use of inhibitors to increase efficiency of crispr/cas insertions |
| WO2023109849A1 (en) * | 2021-12-15 | 2023-06-22 | Wuhan University | Dna polymerase-mediated genome editing |
| EP4689155A1 (en) | 2023-03-29 | 2026-02-11 | Astrazeneca AB | Use of inhibitors to increase efficiency of crispr/cas insertions |
| CN119614679A (zh) * | 2023-09-14 | 2025-03-14 | 厦门大学 | 一款通用型核酸检测平台 |
| CN116949012B (zh) * | 2023-09-20 | 2024-01-02 | 广州瑞风生物科技有限公司 | 一种融合蛋白及其应用 |
| EP4678742A1 (en) | 2024-07-10 | 2026-01-14 | Astrazeneca AB | Improved cas9 proteins |
| WO2026083309A1 (en) | 2024-10-17 | 2026-04-23 | Astrazeneca Ab | Use of inhibitors or modulators of artemis in gene editing |
Family Cites Families (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5543158A (en) | 1993-07-23 | 1996-08-06 | Massachusetts Institute Of Technology | Biodegradable injectable nanoparticles |
| US6007845A (en) | 1994-07-22 | 1999-12-28 | Massachusetts Institute Of Technology | Nanoparticles and microparticles of non-linear hydrophilic-hydrophobic multiblock copolymers |
| US5855913A (en) | 1997-01-16 | 1999-01-05 | Massachusetts Instite Of Technology | Particles incorporating surfactants for pulmonary drug delivery |
| US5895309A (en) | 1998-02-09 | 1999-04-20 | Spector; Donald | Collapsible hula-hoop |
| JP2008078613A (ja) | 2006-08-24 | 2008-04-03 | Rohm Co Ltd | 窒化物半導体の製造方法及び窒化物半導体素子 |
| EP3269395A1 (en) | 2008-11-07 | 2018-01-17 | Massachusetts Institute Of Technology | Aminoalcohol lipidoids and uses thereof |
| US8889394B2 (en) | 2009-09-07 | 2014-11-18 | Empire Technology Development Llc | Multiple domain proteins |
| US9193827B2 (en) | 2010-08-26 | 2015-11-24 | Massachusetts Institute Of Technology | Poly(beta-amino alcohols), their preparation, and uses thereof |
| EP2691443B1 (en) | 2011-03-28 | 2021-02-17 | Massachusetts Institute of Technology | Conjugated lipomers and uses thereof |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| AU2013266968B2 (en) | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| AU2013293270B2 (en) | 2012-07-25 | 2018-08-16 | Massachusetts Institute Of Technology | Inducible DNA binding proteins and genome perturbation tools and applications thereof |
| WO2014033644A2 (en) | 2012-08-28 | 2014-03-06 | Novartis Ag | Methods of nuclease-based genetic engineering |
| PL3360964T3 (pl) * | 2012-12-06 | 2020-03-31 | Sigma-Aldrich Co. Llc | Modyfikacja i regulacja genomu oparta na crispr |
| EP3031921B1 (en) | 2012-12-12 | 2025-03-12 | The Broad Institute, Inc. | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
| CN113355357B (zh) | 2012-12-12 | 2024-12-03 | 布罗德研究所有限公司 | 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化 |
| IL239344B2 (en) * | 2012-12-12 | 2024-06-01 | Broad Inst Inc | Systems engineering, methods and optimal guiding components for sequence manipulation |
| WO2014118272A1 (en) | 2013-01-30 | 2014-08-07 | Santaris Pharma A/S | Antimir-122 oligonucleotide carbohydrate conjugates |
| CN112301024A (zh) * | 2013-03-15 | 2021-02-02 | 通用医疗公司 | 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性 |
| US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
| CA2913234A1 (en) | 2013-05-22 | 2014-11-27 | Northwestern University | Rna-directed dna cleavage and gene editing by cas9 enzyme from neisseria meningitidis |
| CN105492611A (zh) * | 2013-06-17 | 2016-04-13 | 布罗德研究所有限公司 | 用于序列操纵的优化的crispr-cas双切口酶系统、方法以及组合物 |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| SG10201804975PA (en) * | 2013-12-12 | 2018-07-30 | Broad Inst Inc | Delivery, Use and Therapeutic Applications of the Crispr-Cas Systems and Compositions for HBV and Viral Diseases and Disorders |
| EP3470089A1 (en) * | 2013-12-12 | 2019-04-17 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components |
| WO2015089364A1 (en) * | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Crystal structure of a crispr-cas system, and uses thereof |
| JP6793547B2 (ja) * | 2013-12-12 | 2020-12-02 | ザ・ブロード・インスティテュート・インコーポレイテッド | 最適化機能ＣＲＩＳＰＲ−Ｃａｓ系による配列操作のための系、方法および組成物 |
| SG11201701245QA (en) * | 2014-08-27 | 2017-03-30 | Caribou Biosciences Inc | Methods for increasing cas9-mediated engineering efficiency |
| JP2017534294A (ja) * | 2014-11-19 | 2017-11-24 | インスティチュート フォー ベーシック サイエンスＩｎｓｔｉｔｕｔｅ Ｆｏｒ Ｂａｓｉｃ Ｓｃｉｅｎｃｅ | 二つのベクターから発現されたｃａｓ９タンパク質を利用した遺伝子発現調節方法 |
| WO2016106236A1 (en) * | 2014-12-23 | 2016-06-30 | The Broad Institute Inc. | Rna-targeting system |
| US9650617B2 (en) | 2015-01-28 | 2017-05-16 | Pioneer Hi-Bred International. Inc. | CRISPR hybrid DNA/RNA polynucleotides and methods of use |
| WO2016196655A1 (en) * | 2015-06-03 | 2016-12-08 | The Regents Of The University Of California | Cas9 variants and methods of use thereof |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| WO2016205759A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| US20180201912A1 (en) * | 2015-07-14 | 2018-07-19 | The University Of Tokyo | Modified fncas9 protein and use thereof |
| JP7044373B2 (ja) * | 2015-07-15 | 2022-03-30 | ラトガース，ザ ステート ユニバーシティ オブ ニュージャージー | ヌクレアーゼ非依存的な標的化遺伝子編集プラットフォームおよびその用途 |
| WO2017151444A1 (en) * | 2016-02-29 | 2017-09-08 | Agilent Technologies, Inc. | Methods and compositions for blocking off-target nucleic acids from cleavage by crispr proteins |
| EP3426778A1 (en) * | 2016-03-11 | 2019-01-16 | Pioneer Hi-Bred International, Inc. | Novel cas9 systems and methods of use |
| EP3426780A1 (en) * | 2016-03-11 | 2019-01-16 | Pioneer Hi-Bred International, Inc. | Novel cas9 systems and methods of use |
| AU2017257274B2 (en) * | 2016-04-19 | 2023-07-13 | Massachusetts Institute Of Technology | Novel CRISPR enzymes and systems |
| WO2017189336A1 (en) * | 2016-04-25 | 2017-11-02 | The Regents Of The University Of California | Methods and compositions for genomic editing |
| US20190136230A1 (en) * | 2016-05-06 | 2019-05-09 | Juno Therapeutics, Inc. | Genetically engineered cells and methods of making the same |
-
2018
- 2018-11-16 CN CN202510144781.XA patent/CN120310773A/zh active Pending
- 2018-11-16 EP EP18826131.7A patent/EP3710583A1/en active Pending
- 2018-11-16 US US16/763,809 patent/US20210180059A1/en not_active Abandoned
- 2018-11-16 WO PCT/US2018/061680 patent/WO2019099943A1/en not_active Ceased
- 2018-11-16 CN CN201880073647.3A patent/CN111448313A/zh active Pending
- 2018-11-16 JP JP2020526198A patent/JP7423520B2/ja active Active
-
2024
- 2024-01-17 JP JP2024005210A patent/JP7703707B2/ja active Active
- 2024-02-13 US US18/439,897 patent/US20250034562A1/en active Pending
-
2025
- 2025-04-11 JP JP2025065457A patent/JP2025121906A/ja active Pending
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