JP2024118708A - リパーゼ変異体 - Google Patents

リパーゼ変異体 Download PDF

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Publication number
JP2024118708A
JP2024118708A JP2023025140A JP2023025140A JP2024118708A JP 2024118708 A JP2024118708 A JP 2024118708A JP 2023025140 A JP2023025140 A JP 2023025140A JP 2023025140 A JP2023025140 A JP 2023025140A JP 2024118708 A JP2024118708 A JP 2024118708A
Authority
JP
Japan
Prior art keywords
lipase
amino acid
seq
numbering
acid residue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023025140A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024118708A5 (https=
Inventor
貴大 日置
Takahiro Hioki
彰人 川原
Akihito Kawahara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kao Corp
Original Assignee
Kao Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kao Corp filed Critical Kao Corp
Priority to JP2023025140A priority Critical patent/JP2024118708A/ja
Priority to EP24760340.0A priority patent/EP4671373A1/en
Priority to PCT/JP2024/005941 priority patent/WO2024177042A1/ja
Publication of JP2024118708A publication Critical patent/JP2024118708A/ja
Publication of JP2024118708A5 publication Critical patent/JP2024118708A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
JP2023025140A 2023-02-21 2023-02-21 リパーゼ変異体 Pending JP2024118708A (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2023025140A JP2024118708A (ja) 2023-02-21 2023-02-21 リパーゼ変異体
EP24760340.0A EP4671373A1 (en) 2023-02-21 2024-02-20 LIPASE VARIANT
PCT/JP2024/005941 WO2024177042A1 (ja) 2023-02-21 2024-02-20 リパーゼ変異体

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2023025140A JP2024118708A (ja) 2023-02-21 2023-02-21 リパーゼ変異体

Publications (2)

Publication Number Publication Date
JP2024118708A true JP2024118708A (ja) 2024-09-02
JP2024118708A5 JP2024118708A5 (https=) 2025-12-24

Family

ID=92501350

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023025140A Pending JP2024118708A (ja) 2023-02-21 2023-02-21 リパーゼ変異体

Country Status (3)

Country Link
EP (1) EP4671373A1 (https=)
JP (1) JP2024118708A (https=)
WO (1) WO2024177042A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025126926A1 (ja) * 2023-12-15 2025-06-19 花王株式会社 リパーゼ変異体

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2635288B2 (ja) 1993-03-11 1997-07-30 花王株式会社 漂白剤組成物及び漂白洗浄剤組成物
JP3512981B2 (ja) 1997-05-19 2004-03-31 花王株式会社 耐熱性アルカリセルラ−ゼ、それを生産する微生物及びその製造方法
JP3492935B2 (ja) 1999-04-02 2004-02-03 花王株式会社 プラスミドベクター
JP3872373B2 (ja) 2002-04-18 2007-01-24 花王株式会社 粉末洗浄剤組成物
JP4485341B2 (ja) 2004-12-20 2010-06-23 花王株式会社 組換え微生物
WO2013171241A1 (en) 2012-05-16 2013-11-21 Novozymes A/S Compositions comprising lipase and methods of use thereof
WO2014009473A1 (en) 2012-07-12 2014-01-16 Novozymes A/S Polypeptides having lipase activity and polynucleotides encoding same
JP7317811B2 (ja) * 2017-09-27 2023-07-31 ノボザイムス アクティーゼルスカブ リパーゼ変異体及びかかるリパーゼ変異体を含むマイクロカプセル組成物
JP2019182911A (ja) 2018-04-02 2019-10-24 花王株式会社 食器及び／又は台所周りの硬質物品用液体洗浄剤組成物
JP7262333B2 (ja) 2019-07-22 2023-04-21 花王株式会社 硬質物品の洗浄方法
CN115038791A (zh) 2020-01-27 2022-09-09 花王株式会社 重链抗体的重链可变区的制造方法
JPWO2022250123A1 (https=) * 2021-05-28 2022-12-01

Also Published As

Publication number Publication date
EP4671373A1 (en) 2025-12-31
WO2024177042A1 (ja) 2024-08-29

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