JP2024061201A - Mitophagy activators - Google Patents

Abstract

[Problem] To provide a plant-derived ingredient having a mitophagy activating effect that can be incorporated into topical skin preparations. [Solution] A mitophagy activator containing an extract of a plant belonging to the genus Paeonia in the family Paeoniaceae, an extract of a plant belonging to the genus Prunus in the family Rosaceae, and an extract of a plant belonging to the genus Perilla in the family Lamiaceae. [Selected Figure] Figure 1

Description

The present invention relates to a mitophagy activator that contains as active ingredients a mixture of extracts of plants belonging to the genus Paeoniae in the family Paeoniaceae, extracts of plants belonging to the genus Prunus in the family Rosaceae, and extracts of plants belonging to the genus Lamiaceae in the family Lamiaceae.

It has been known that when the skin (including the scalp) is exposed to ultraviolet rays, inflammation or oxidative stress is induced, and this inflammation or oxidative stress is the cause of wrinkles, age spots, etc. It is also known, for example from Non-Patent Document 1, that when inflammation and oxidative stress occur in the skin due to ultraviolet rays, hair growth is suppressed.

Yuki Inokuchi, FREGRANCE JOURNAL 2021-2, pp. 29-34

JP 2015-143205 A

In view of the above background technology, there is a demand for an active ingredient that can be incorporated into topical skin preparations (cosmetics, quasi-drugs, and topical medicines) and that suppresses inflammation or oxidative stress of the skin caused by factors such as ultraviolet rays.

In light of the problems with the prior art, the present inventors conducted intensive research and discovered that a mixture of extracts of plants belonging to the genus Paeoniae in the family Paeoniaceae, extracts of plants belonging to the genus Prunus in the family Rosaceae, and extracts of plants belonging to the genus Lamiaceae in the family Lamiaceae activates mitophagy in skin (including scalp) cells and suppresses oxidative stress.

It has been known from Patent Document 1 that a mixture of extracts of plants belonging to the genus Paeoniae (Paeoniae Family), extracts of plants belonging to the genus Prunus (Rosaceae Family), and extracts of plants belonging to the genus Lamiaceae (Lamiaceae Family) has antioxidant, anti-inflammatory, and anti-androgenic effects, but it was not known that the mixture activates mitophagy in the skin.

The present invention relates to a mitophagy activation inhibitor containing an extract of a plant belonging to the genus Paeoniae (Paeoniaceae), an extract of a plant belonging to the genus Prunus (Prunus) (Rosaceae), and an extract of a plant belonging to the genus Lamiaceae (Lamiaceae).The present invention also relates to an external skin preparation for hair growth formulated with a mitophagy activation inhibitor containing an extract of a plant belonging to the genus Paeoniae (Paeoniaceae), an extract of a plant belonging to the genus Prunus (Prunus) (Rosaceae), and an extract of a plant belonging to the genus Lamiaceae (Lamiaceae).

The present invention relates to a mitophagy activator that contains an extract of a plant belonging to the genus Paeoniae in the family Paeoniaceae, an extract of a plant belonging to the genus Prunus in the family Rosaceae, and an extract of a plant belonging to the genus Lamiaceae in the family Lamiaceae. Since mitophagy is a selective decomposition function of mitochondria (particularly abnormal mitochondria), the mitophagy activating effect of the mixture of plant extracts can suppress the generation of active oxygen caused by abnormal mitochondria, thereby providing an external skin preparation that suppresses oxidative stress on the skin, etc.

FIG. 1 shows the mitophagy activating effect of a mixture of plant extracts according to the present invention.

The plant-derived extract material used in the present invention may be any species of plant belonging to the genus Paeoniae in the family Paeoniaceae, the genus Prunus in the family Rosaceae, or the genus Lamiaceae in the family Lamiaceae.

The plant species of the genus Paeonia in the family Paeoniaceae is not particularly limited, and examples thereof include peony (Paeonia lactiflora), mountain peony (Paeonia japonica), red mountain peony (Paeonia obovata), and peony (Paeonia suffruticosa). In addition, the whole plant, leaves, flowers, stems, seeds, fruits, roots, etc. of the plant may be used, but the use of the whole plant or roots is preferred.

The species of the plant of the genus Prunus in the family Rosaceae is not particularly limited, and examples thereof include peach (Prunus persica), apricot (Prunus vulgaris), plum (Prunus mume), and plum (Prunus salicina). The whole plant, leaves, flowers, stems, seeds, fruits, roots, etc. of the plant may be used, but the use of the whole plant or seeds is preferred.

Plant species of the genus Perilla in the family Lamiaceae are not particularly limited, and examples include perilla and perilla. In addition, perilla generally includes those with the scientific name Perilla frutescens var. crispa or Perilla frutescens var. Britton, and examples include Aojiso, Chirimen shiso, Aka shiso, Madarajiso, Katamen shiso, Chirimen aojiso, or variants, subspecies, or hybrids thereof, but the present invention is not limited thereto. In addition, the whole plant, leaves, inflorescences, stems, seeds, fruits, roots, etc. of the plant may be used, but the use of the whole plant or leaves is preferred.

To prepare the extract, first, the part of each plant to be used is washed with water if necessary to remove foreign matter, and then either left as is or dried, and then chopped or crushed as necessary, and then contacted with the extraction solvent to carry out the extraction. Extraction can be carried out by contacting with the extraction solvent according to standard methods such as the immersion method, but it is also possible to use supercritical extraction methods other than the immersion method.

Extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform, which may be used alone or in combination of two or more.

Among the above-mentioned extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention from the viewpoint of the effectiveness and skin irritation of the resulting mixture of plant extracts, and also from the viewpoint of the wide range of application to skin topicals. Preferred examples of hydrophilic solvents include the use of water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol, propanediol, and glycerin) alone, or a mixed solvent of water and lower alcohols (particularly ethanol), or a mixed solvent of water and polyhydric alcohols (particularly 1,3-butylene glycol, propanediol, and glycerin). When a mixed solvent is used, the mixing ratio is preferably in the range of 1:1 to 20:1 by volume (hereinafter the same) for a mixed solvent of water and 1,3-butylene glycol, 1:1 to 25:1 for a mixed solvent of water and ethanol, and 1:1 to 20:1 for a mixed solvent of water and glycerin.

The weight ratio of each plant part to be used and the extraction solvent is preferably 1:1 to 1:50.

When preparing the extract, there is no particular limit to its pH, but it is generally preferable to set it in the range of 3 to 9. In this sense, if necessary, an alkalinity regulator such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity regulator such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid may be added to the extraction solvent to adjust the pH to the desired level.

The extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used, but for example, when the solvent is water, a lower alcohol or a polyhydric alcohol, or a mixed solution of water and a lower alcohol or water and a polyhydric alcohol, the extraction temperature is preferably in the range of 0°C to 90°C. The extraction time is preferably 1 to 168 hours (1 hour to 1 week).

In addition, prior to or in parallel with the extraction process of the present invention, the extract may be subjected to a hydrolysis process as necessary. This may improve the storage stability of the extract, allowing the extract to be used more effectively as a cosmetic ingredient.

When the extract is subjected to enzymatic hydrolysis, the enzymes used may be one or more selected from any of the following enzyme groups: proteolytic enzymes such as actinase, papain, and pepsin; starch-degrading enzymes such as glucoamylase, α-amylase, and β-amylase; and cellulose-degrading enzymes such as cellulase, hemicellulase, and pectinase. It is more preferable to use a combination of one or more enzymes selected from each of these enzyme groups.

The amount of enzyme added is, for example, preferably in the range of 0.01 to 10% by weight in total, and more preferably in the range of 0.1 to 2.0% by weight, based on the solid content of the part of the plant used.

The extracts prepared as described above are mixed and generally adjusted to a pH of 3 to 8, and may be used as is as an ingredient in topical skin preparations, or may be concentrated under reduced pressure or used at the desired concentration. The extracts may also be dried using standard methods such as spray drying.

The mixing ratio of each extract, which can be adjusted as described above, may be set appropriately taking into consideration effectiveness and stability. For example, the solid content ratio of Lamia plant extract to Paeonia suffruticosa plant extract may be 1:1-5, or Lamia plant extract to Cherry plant extract may be 1:0.9-3.

Examples of skin external preparations (cosmetics, quasi-drugs, external medicines) containing the mixture of plant extracts according to the present invention include, but are not limited to, milky lotions, creams, lotions, essences, packs, lipsticks, foundations, sheet masks, liquid foundations, makeup press powders, blushers, face powders, facial cleansers, body shampoos, hair shampoos, bath additives, soaps and other cleansing cosmetics, hair growth agents, hair restorers, etc.

When the mixture of plant extracts according to the present invention is incorporated into a skin care topical preparation, the amount of each extract in the mixture is generally in the range of 0.001 to 5.0% by weight, in terms of the solid content of each extract. When the mixture is incorporated into a skin topical preparation for hair, the amount of each extract in the mixture is generally in the range of 0.001 to 5.0% by weight, in terms of the solid content of each extract.

When used in topical skin preparations for hair, it is suggested that a synergistic effect in hair growth can be achieved by combining it with a hair growth ingredient. For example, ingredients that can be expected to have a synergistic effect in hair growth include minoxidil, cyproterone acetate, pentadecanoic acid glyceride, 6-aminobenzylpurine (cytopurine), adenosine, trans-3,4'-dimethyl 3-hydroxyflavanone (t-flavanone), Swertia japonica extract, Sophora root extract, Capsicum annuum extract, hinokitiol, photosensitizers, pantothenic acid and its derivatives, vitamin E and its derivatives, nicotinic acid derivatives (nicotinamide, etc.), carpronium chloride, female hormones (ethynyl estradiol, estrone, etc.), salicylic acid, dipotassium glycyrrhizinate, hinokitiol, benzalkonium chloride, isopropyl methylphenol, l-menthol, and 1-methyl-2-methyl-2-propanol. , pyridoxine hydrochloride (vitamin B6), diphenhydramine hydrochloride, potassium (ascorbyl/tocopheryl) phosphate, thioxolone, camphor, resorcin, extract of Trifolium Ramatum root, bis-alkaloids obtained from Trifolium Ramatum, licorice extract, snail flower extract, chamomile extract, fermented royal jelly, fermented lotus seed, ginkgo extract, paldarco bark extract, gentiana extract, ginseng extract, fermented soy milk, Salicornia herb extract, bamboo shoot extract, kudzu root extract, burdock extract, Laminaria acutiloba extract, clove extract, collagen, amino acids, and vitamins, and any one or more of these may be blended.

Furthermore, ingredients used in topical skin preparations containing the mixture of plant extracts according to the present invention, such as oily ingredients, surfactants (synthetic or natural), moisturizers, thickeners, emulsifiers or emulsifier assistants, preservatives/bactericides, powder ingredients, UV absorbers, antioxidants, pigments, fragrances, and other physiologically active ingredients, can be appropriately blended as necessary. It is also possible to combine the mixture with other physiologically active ingredients derived from natural products and blend them into topical skin preparations.

Examples of oily components include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, and other plant-derived oils and fats such as squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, and paraffin wax. , squalane, and other hydrocarbons; myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid, and other fatty acids; lauryl alcohol, cetanol, pantothenyl alcohol, stearyl alcohol, and other higher alcohols; isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (octyldodecyl stearate, etc.), as well as synthetic esters and synthetic triglycerides.

Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Anionic surfactants such as phenyl alkyl phenyl ether phosphate; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkyl benzyl ammonium salts, alkyl pyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethyl imidazolinium salts, N,N-dialkyl morpholinium salts, and polyethylene polyamine fatty acid amide salts; amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethyl ammonio betaine, N,N,N-trialkyl-N-alkylene ammonio carboxy betaine, N-acylamidopropyl-N', N'-dimethyl-N'-β-hydroxypropyl ammonio sulfobetaine, etc. can be used.

As emulsifiers and/or emulsifier aids, stevia derivatives such as enzyme-treated stevia, saponin or its derivatives, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or its esters, lactose, soy-derived water-soluble polysaccharides, soy-derived protein and polysaccharide complexes, lanolin or its derivatives, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and its derivatives, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented grains (wheat, beans, millet, etc.), etc. can also be blended.

Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, 1,2-pentanediol, 1,2-hexanediol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, estradiol, various amino acids and their derivatives.

Examples of thickeners include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin and aloe polysaccharides; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, and hydroxypropylcellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid/methacrylic acid copolymers; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, nicotinamide, and riboflavin or its derivatives.

Examples of preservatives and disinfectants include urea; benzoic acid or its salts, paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, alkylisoquinolinium bromide, resorcinol, and jamaica. These include ethanol (imidazolidinyl urea), isopropyl methylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, 1,2-hexanediol, 1,2-octanediol, ethylhexylglycerin, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark distillate, fermented radish liquid, ethanol derived from plants such as sugar cane and corn, or 1,3-butylene glycol.

Cell activators include pantothenyl alcohol, menthol, dl-menthol, and gamma-oryzanol.

Anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, etc.

Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, grain powder (rice, wheat, corn, millet, etc.), and bean powder (soybean, adzuki bean, etc.).

Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc.

Examples of antioxidants include butyl hydroxyanisole, butyl hydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A and its derivatives (retinol palmitate, etc.), etc.

In addition, it is also possible to use in combination with ingredients derived from natural products such as the following plants or microorganisms. For example, collagen or its hydrolysate, yeast extract or hydrolysate, lactic acid bacteria culture, Gramineae plants, Cruciferae plants, Theaceae plants, Rosaceae plants, Paeoniaceae plants, Rutaceae plants, Amaranthaceae plants, Zosteraeae plants, Leguminosae plants, Asteraceae plants, Fabaceae plants, Malvaceae plants, Gentianaceae plants, Lamiaceae plants, Nelumbaceae plants, Cucurbitaceae plants, Araliaceae plants, Solanaceae plants, Bignoniaceae plants, Actinidiaceae plants, Mulberry plants, Iridaceae plants, Campanulaceae plants, Oleaceae plants, Actinidiaceae plants, Mulberry plants, Rhamnaceae plants, Orchidaceae plants, Anacardiaceae plants extracts or hydrolysates or fermented products of one or more plants selected from the family Gardenia, Valencaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae; extracts or hydrolysates or fermented products of one or more seaweeds selected from the family Laminaria, Laminaceae, and Ulvulaceae; jellyfish (autolyzed products of moon jellyfish, Nomura's jellyfish, etc.); hydrolysates or fermented products of hyaluronic acid; and extracts or hydrolysates or fermented products of royal jelly.

As the components derived from plants of the Gramineae family, in particular, hydrolyzed rice leaves, hydrolyzed rice extracts, hydrolyzed rice bran extracts, hydrolyzed germinated brown rice, fermented rice liquid, sake lees extracts derived from sake, extracts of bamboo shoot skin from Madake or Moso bamboo, and fermented pearl barley seeds are preferred. As the components derived from plants of the Brassicaceae family, in particular, extracts of seeds of Hakugai, Ogai or Kokugai or their hydrolyzates or fermented products are preferred. As the components derived from plants of the Theaceae family, in particular, green tea (Yabukita, Samidori, Asahi, Goko, Ujimidori, Kyomidori, Ujihikari, Samidori, Benifuki, etc.) and black tea (Darjeeling, Assam, Ceylon, Earl Grey, Honey-scented black tea, etc.) are preferred. As the components derived from plants of the Rosaceae family, extracts of Damask rose flowers, strawberry flower extracts, and cherry blossom or leaf extracts are preferred. As the components derived from plants of the Amaranthaceae family, in particular, Salicornia herbacea extract is preferred. As the component derived from a Zosteraeae plant, particularly preferred is an extract of Zostera marina or Zostera quinata. As the component derived from a Leguminosae plant, particularly preferred is an extract of white soybean or black soybean or its hydrolysate, or fermented soy milk, an extract of adzuki bean, an extract of red clover, or an extract of pueraria lobata root. As the component derived from a Asteraceae plant, particularly preferred is an extract of burdock root, an extract of sunflower sprouts, an extract of Lacertilia arbutifolia, an extract of arnica, or an extract of chamomile flower. As the component derived from a Malvaceae plant, particularly preferred is a fermented product of hibiscus, Rose of Sharon, or Hibiscus. As the component derived from a Gentianaceae plant, a gentian extract is preferred. As the component derived from a Lamiaceae plant, a fruit extract of Acanthus arbutifolia is preferred. As the component derived from a Nelumbinaceae plant, particularly preferred is an extract of lotus flower or lotus seed, or a fermented product of lotus seed. As the component derived from a Cucurbitaceae plant, particularly preferred is a loofah extract. As the component derived from a Araliaceae plant, a ginseng extract or a fermented product is preferred. Examples of components derived from Solanaceae plants include extracts of eggplants (long eggplant, water eggplant, rice eggplant, Kamo eggplant, etc.). Examples of components derived from Bignoniaceae plants include Pau d'Arco bark extract. Examples of components derived from Actinidiaceae plants include immature kiwi extract. Examples of components derived from Mulberry plants include Morus alba extract, mulberry fruit extract, and fig fruit or bark extract. Examples of components derived from Rhamnaceae plants include jujube fruit extract. Examples of components derived from Iridaceae plants include saffron. Examples of components derived from Campanulaceae plants include an extract or hydrolysate of the root of Lycoris nigra. Examples of components derived from Anacardiaceae plants include mango fruit extract. Examples of components derived from Garcinia arbutifolia plants include mangosteen fruit extract. Examples of components derived from Valensaceae plants include cherimoya fruit extract. As the component derived from a plant of the Rutaceae family, an extract of Unshu mandarin orange, an extract of bergamot fruit, an extract of grapefruit or a fruit of Banpeiyu (including immature fruit), an extract containing flavonoids and their glycosides contained in a plant of grapefruit or Hassaku, or an extract of a seed of Japanese pepper is preferable. As the component derived from a plant of the Liliaceae family, an extract of Daylily, Daylily, Casablanca, Madonna lily, or Lilium longiflorum is preferable. As the component derived from a plant of the Crassulaceae family, an extract or fermented product of Rhodiola rosea is particularly preferable. As the component derived from a plant of the Oleaceae family, an extract of Jasmine flower is particularly preferable. As the plant of the Cupressaceae family, an extract of Juniperus communis fruit is particularly preferable. As the component derived from a plant of the Myrtaceae family, an extract of guava leaf is particularly preferable. As the plant of the Orchidaceae family, an extract of the root (white part) of Bletilla serrata is particularly preferable. As the component derived from a plant of the Convolvulaceae family, a sweet potato extract or its fermentation product, or a sweet potato shochu lees extract or its fermentation product is preferable. As the component derived from seaweed of the Laminaceae family, a kelp extract is particularly preferable, as the component derived from seaweed of the Mirinaceae family, a Katamen Kirinsai extract is particularly preferable, and as the component derived from seaweed of the Ulva family, an Ulva pertusa extract is particularly preferable. As the component derived from seaweed of the Funoriaceae family, a Funori extract is particularly preferable.

The present invention will now be described in more detail with reference to manufacturing examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts are by weight, and all percentages are by weight.

Production Example 1. Mixture solution of plant extracts The roots of peony of the genus Paeoniae were chopped and dried, 50 g of the dried product was immersed in 250 g of purified water, and 250 g of 1,3-butylene glycol was added. After the addition, extraction was performed at 4°C. The extract was filtered to obtain 442 g of a brown transparent peony extract solution (solid content concentration 3.57%). In addition, peach seeds (peach kernel) of the genus Prunus of the family Rosaceae were dried and crushed, 50 g of the crushed product was immersed in 250 g of purified water, and 250 g of 1,3-butylene glycol was added. After the addition, extraction was performed at 4°C. The extract was filtered to obtain 493 g of a pale yellow transparent peach kernel extract solution (solid content concentration 1.74%). In addition, perilla leaves of the genus Lamiaceae were dried, 25 g of the dried product was immersed in 250 g of purified water, and 250 g of 1,3-butylene glycol was added. After the addition, extraction was carried out at 4° C. The extract was then filtered to obtain 448 g of a brown, transparent perilla extract solution (solid content concentration: 1.02%). The above three plant extracts were mixed, and the insoluble matter was filtered to obtain 1,230 g of a brown, transparent extract mixture solution (hereinafter referred to as the “extract mixture solution”) (solid content concentration: 1.54%).

Test Example 1. Evaluation test of mitophagy activation effect Human-derived hair follicle papilla cells (HFDPC) were seeded on a 96-well microplate and cultured for 24 hours. After the culture, a medium containing the extract mixture solution of Production Example 1 as a sample solution was added and cultured. Here, the concentration of the extract mixture solution was adjusted so that the final concentration as a solution in the medium was 1.0%. After adding the sample solution and culturing for 48 hours, the supernatant was removed, and the medium was replaced with a carbonyl cyanide-m-chlorophenylhydrazone (CCCP)-containing medium and cultured for a certain period of time. Thereafter, the CCCP-containing medium was removed, and mitophagy activity was measured using a Cyto-ID Autophagy Detection Kit. In addition, DNA staining with Hoechst 33342 was performed on cells cultured in the same manner on a separate plate, and the fluorescence intensity (excitation wavelength 355 nm, fluorescence wavelength 460 nm) was measured to determine the amount of DNA, and the mitophagy activity per amount of DNA was calculated. In addition, a control was set up in which HFDPCs were cultured in a medium to which neither the sample solution nor CCCP was added, and a CCCP-treated control was set up in which HFDPCs were cultured without the addition of the sample solution and with the addition of CCCP, and the mitophagy activity and DNA amount for each were measured, and the mitophagy activity rate (%) per DNA amount was calculated as a relative value when the control was set at 100. The above-mentioned CCCP is an uncoupling agent, and when added to cells, it prevents a proton gradient from occurring within mitochondria, causing them to be recognized as "abnormal mitochondria," and is a reagent that can induce mitophagy.

The results of Test Example 1 are shown in Figure 1. As shown in Figure 1, it was confirmed that the mixture of plant extracts according to the present invention exhibits a mitophagy activation effect. Since Test Example 1 showed an effect of activating mitophagy in dermal papilla cells, the mixture of plant extracts according to the present invention can suppress the generation of reactive oxygen species caused by abnormal mitochondria in dermal papilla cells and suppress oxidative damage to the cells. As a result, this suggests an effect of protecting dermal papilla cells that regulate the hair cycle and normalizing the hair cycle.

In addition, it is expected that the mitophagy activation effect will suppress oxidative damage to cells other than hair papilla cells, thereby suppressing cell damage and aging caused by ultraviolet rays, etc.

Formulation Example 1. Lotion [Ingredients] Parts Jojoba oil 1.0
Polyoxyethylene (5.5) Cetyl Alcohol 5.0
Methylparaben 0.1
Niacinamide 3.0
Ascorbic acid glucoside 2.0
Tranexamic acid 2.0
ε-aminocaproic acid 0.1
Sulfur 0.2
Estradiol 0.1
Mixture of extracts from Preparation Example 1 1.0
Glycerin 5.0
1,3-butylene glycol 5.0
Sodium citrate 0.2
Sodium metabisulfite 0.1
d-Camphor 0.1
Purified water (enough to make the total amount 100 parts)

Formulation Example 2. Hair growth agent [Ingredients] Adenosine 1.0
Mixture of extracts from Preparation Example 1 1.0
Nicotinamide 1.0
Tocopherol acetate 0.1
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Ethanol 20.0
Purified water (enough to make the total amount 100 parts)

Formulation Example 3. Hair Growth Agent A hair growth agent was obtained in the same manner as in Formulation Example 1, except that, among the ingredients in Formulation Example 2, minoxidil was used instead of adenosine.

Formulation Example 4. Hair growth agent A hair growth agent was obtained in the same manner as in Formulation Example 9, except that, among the ingredients of Formulation Example 2, 6-benzylaminopurine was used instead of adenosine.

Formulation Example 5. Hair growth agent [Ingredients] Parts l-menthol 0.8
Dipotassium glycyrrhizinate 0.1
Isopropyl methylphenol 0.1
Mixture of extracts from Preparation Example 1 2.0
Soy lecithin 0.2
1,3-butylene glycol 10.0
Ethanol 10.0
Tamasakizurafuji extract 0.01
Carrot extract 0.1
Bamboo shoot skin extract 3.0
Brown algae extract 3.0
Royal jelly fermented extract 3.0
Burdock extract 3.0
Kudzu root extract 3.0
Purified water (enough to make the total amount 100 parts)

Formulation Example 6. Hair growth cream [Ingredients] Parts Liquid paraffin 15.0
Vaseline 15.0
White beeswax 2.0
Mixture of extracts from Preparation Example 1 1.0
Brown algae extract 0.3
Carboxyvinyl polymer 0.1
Xanthan gum 0.1
Swertia japonica extract 0.3
Diphenhydramine hydrochloride 0.1
(Ascorbyl/Tocopheryl) Phosphate Potassium 0.1
Glycerin 5.0
1,3-butylene glycol 2.0
Polyoxyethylene hydrogenated castor oil 3.0
Chelating agent 0.1
Preservatives: 0.1
Purified water (enough to make the total amount 100 parts)

Formulation Example 6. Hair growth shampoo [Ingredients] Parts Sodium N-coconut oil fatty acid methyl taurate 10.0
Sodium polyoxyethylene (3) alkyl ether sulfate 20.0
Lauryl dimethylaminoacetate betaine 10.0
Coconut oil fatty acid diethanolamide 4.0
Methylparaben 0.1
Citric acid 0.1
Mixture of extracts from Preparation Example 1 2.0
Collagen 5.0
Capsicum extract 0.05
Zostera extract 5.0
Salicornia extract 5.0
Kudzu root extract 5.0
Burdock extract 5.0
1,3-butylene glycol 2.0
Purified water (enough to make the total amount 100 parts)

Example 7. Hair rinse [Ingredients] Parts Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.0
Stearyl alcohol 1.0
Methylparaben 0.1
Mixture of extracts from Preparation Example 1 2.0
1,3-butylene glycol 5.0
Purified water (enough to make the total amount 100 parts)

Claims (2)

A mitophagy activator containing an extract of a plant belonging to the genus Paeonia in the family Paeoniaceae, an extract of a plant belonging to the genus Prunus in the family Rosaceae, and an extract of a plant belonging to the genus Perilla in the family Lamiaceae. A topical skin preparation for hair growth containing the mitophagy activator described in claim 1.