JP2023542820A - 細胞培養成績を改善し、アスパラギン配列バリアントを軽減するためのアスパラギン供給戦略 - Google Patents

細胞培養成績を改善し、アスパラギン配列バリアントを軽減するためのアスパラギン供給戦略 Download PDF

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JP2023542820A
JP2023542820A JP2023513579A JP2023513579A JP2023542820A JP 2023542820 A JP2023542820 A JP 2023542820A JP 2023513579 A JP2023513579 A JP 2023513579A JP 2023513579 A JP2023513579 A JP 2023513579A JP 2023542820 A JP2023542820 A JP 2023542820A
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asparagine
cell culture
fed
antibody
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JP2023542820A5 (enExample
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サンドラ ベヌン－セラーノ
ショーン エム． ローレンス
エイミー エス． ジョンソン
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Regeneron Pharmaceuticals Inc
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
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    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
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    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Biophysics (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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JP2023513579A 2020-08-31 2021-08-27 細胞培養成績を改善し、アスパラギン配列バリアントを軽減するためのアスパラギン供給戦略 Pending JP2023542820A (ja)

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US202063072740P 2020-08-31 2020-08-31
US202063072745P 2020-08-31 2020-08-31
US63/072,745 2020-08-31
US63/072,740 2020-08-31
PCT/US2021/047870 WO2022047108A1 (en) 2020-08-31 2021-08-27 Asparagine feed strategies to improve cell culture performance and mitigate asparagine sequence variants

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JP2023542820A true JP2023542820A (ja) 2023-10-12
JP2023542820A5 JP2023542820A5 (enExample) 2024-09-03

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US (2) US12163122B2 (enExample)
EP (1) EP4204578A1 (enExample)
JP (1) JP2023542820A (enExample)
KR (1) KR20230058094A (enExample)
CN (1) CN116209772A (enExample)
AU (1) AU2021332355A1 (enExample)
BR (1) BR112023003273A2 (enExample)
CA (1) CA3192999A1 (enExample)
CL (1) CL2023000576A1 (enExample)
CO (1) CO2023003662A2 (enExample)
IL (1) IL300828A (enExample)
MX (1) MX2023002417A (enExample)
TW (1) TW202432837A (enExample)
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CN114901794B (zh) 2019-11-15 2026-05-01 隆萨有限公司 用于生产接种物的过程和系统
CN114836367A (zh) * 2022-04-28 2022-08-02 上海东富龙生物试剂有限公司 用于hek293细胞培养及腺病毒、腺相关病毒复制扩增的化学成分限定培养基

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US10526631B1 (en) * 2016-03-15 2020-01-07 Ares Trading S.A. Method of reducing serine for asparagine misincorporation

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BR112023003273A2 (pt) 2023-05-02
IL300828A (en) 2023-04-01
CA3192999A1 (en) 2022-03-03
MX2023002417A (es) 2023-03-22
US12163122B2 (en) 2024-12-10
US20250066718A1 (en) 2025-02-27
CL2023000576A1 (es) 2023-09-29
CO2023003662A2 (es) 2023-04-05
EP4204578A1 (en) 2023-07-05
US20220064591A1 (en) 2022-03-03
KR20230058094A (ko) 2023-05-02
TW202432837A (zh) 2024-08-16
TW202225398A (zh) 2022-07-01
AU2021332355A1 (en) 2023-03-23
CN116209772A (zh) 2023-06-02

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