JP2022511206A5 - - Google Patents

Info

Publication number
JP2022511206A5
JP2022511206A5 JP2020572440A JP2020572440A JP2022511206A5 JP 2022511206 A5 JP2022511206 A5 JP 2022511206A5 JP 2020572440 A JP2020572440 A JP 2020572440A JP 2020572440 A JP2020572440 A JP 2020572440A JP 2022511206 A5 JP2022511206 A5 JP 2022511206A5
Authority
JP
Japan
Prior art keywords
mutation
amino acid
functionally equivalent
substitution
substitution mutation
Prior art date
Application number
JP2020572440A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022511206A (ja
JPWO2020117968A5 (https=
JP7527208B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2019/064524 external-priority patent/WO2020117968A2/en
Publication of JP2022511206A publication Critical patent/JP2022511206A/ja
Publication of JPWO2020117968A5 publication Critical patent/JPWO2020117968A5/ja
Publication of JP2022511206A5 publication Critical patent/JP2022511206A5/ja
Application granted granted Critical
Publication of JP7527208B2 publication Critical patent/JP7527208B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2020572440A 2018-12-05 2019-12-04 ポリメラーゼ、組成物、および使用方法 Active JP7527208B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862775662P 2018-12-05 2018-12-05
US62/775,662 2018-12-05
PCT/US2019/064524 WO2020117968A2 (en) 2018-12-05 2019-12-04 Polymerases, compositions, and methods of use

Publications (4)

Publication Number Publication Date
JP2022511206A JP2022511206A (ja) 2022-01-31
JPWO2020117968A5 JPWO2020117968A5 (https=) 2022-09-13
JP2022511206A5 true JP2022511206A5 (https=) 2022-09-13
JP7527208B2 JP7527208B2 (ja) 2024-08-02

Family

ID=69137980

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020572440A Active JP7527208B2 (ja) 2018-12-05 2019-12-04 ポリメラーゼ、組成物、および使用方法

Country Status (14)

Country Link
US (4) US11001816B2 (https=)
EP (1) EP3891275A2 (https=)
JP (1) JP7527208B2 (https=)
KR (1) KR20210098844A (https=)
CN (1) CN112639090A (https=)
AU (1) AU2019391093B2 (https=)
BR (1) BR112020026655A2 (https=)
CA (1) CA3103739A1 (https=)
IL (2) IL279423B (https=)
MX (1) MX2020013357A (https=)
MY (1) MY204767A (https=)
SG (1) SG11202012559SA (https=)
WO (1) WO2020117968A2 (https=)
ZA (1) ZA202007839B (https=)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11104888B2 (en) 2018-10-31 2021-08-31 Illumina, Inc. Polymerases, compositions, and methods of use
EP3891275A2 (en) 2018-12-05 2021-10-13 Illumina, Inc. Polymerases, compositions, and methods of use
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
CN114829369A (zh) 2019-11-27 2022-07-29 伊鲁米纳剑桥有限公司 含环辛四烯的染料和组合物
CA3165571C (en) 2019-12-23 2023-02-07 Singular Genomics Systems, Inc. Methods for long read sequencing
IL294459A (en) 2019-12-31 2022-09-01 Singular Genomics Systems Inc Polynucleotide barcodes for long read sequencing
EP4117682B1 (en) 2020-04-24 2025-06-04 Singular Genomics Systems, Inc. Modified nucleotides and uses thereof
IL316161A (en) 2020-06-22 2024-12-01 Illumina Cambridge Ltd Nucleotides and nucleosides with a 3' masking group
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
US12054506B2 (en) 2021-03-19 2024-08-06 Singular Genomics Systems, Inc. Reducing agents and uses thereof
JP2024516191A (ja) 2021-05-05 2024-04-12 イルミナ ケンブリッジ リミテッド ビス-ホウ素縮合複素環を含有する蛍光色素及び配列決定における使用
US20220403351A1 (en) 2021-06-18 2022-12-22 Element Biosciences, Inc. Engineered polymerases
US12077789B2 (en) * 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
CA3222937A1 (en) 2021-12-29 2023-07-06 Jonathan Mark Boutell Methods of nucleic acid sequencing using surface-bound primers
US12006518B2 (en) 2022-02-18 2024-06-11 Element Biosciences, Inc. Engineered polymerases with reduced sequence-specific errors
US20230304086A1 (en) 2022-03-28 2023-09-28 Illumina Cambridge Limited Labeled avidin and methods for sequencing
EP4499872B1 (en) 2022-03-29 2026-04-29 Illumina, Inc. Systems and methods of sequencing polynucleotides
CA3223037A1 (en) 2022-03-30 2023-10-05 Oliver MILLER Methods for chemical cleavage of surface-bound polynucleotides
AU2023244351A1 (en) 2022-03-31 2024-01-18 Illumina, Inc. Nucleosides and nucleotides with 3' vinyl blocking group useful in sequencing by synthesis
US20230357845A1 (en) 2022-03-31 2023-11-09 Illumina, Inc. Compositions and methods for improving sequencing signals
CA3251383A1 (en) 2022-05-31 2023-12-07 Illumina, Inc NUCLEIC ACID COMPOSITIONS AND SEQUENCE METHODS
WO2024003087A1 (en) 2022-06-28 2024-01-04 Illumina, Inc. Fluorescent dyes containing fused tetracyclic bis-boron heterocycle and uses in sequencing
WO2024039652A1 (en) 2022-08-16 2024-02-22 Aldevron, L.L.C. Cell-free method of producing synthetic circular nucleic acid
CA3246518A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Compositions and methods for reducing photolesions during sequencing
US20240141427A1 (en) * 2022-09-30 2024-05-02 Illumina, Inc. Polymerases, compositions, and methods of use
WO2024123866A1 (en) 2022-12-09 2024-06-13 Illumina, Inc. Nucleosides and nucleotides with 3´ blocking groups and cleavable linkers
CA3246545A1 (en) 2022-12-22 2024-06-27 Illumina, Inc. PALLADIUM CATALYST COMPOSITIONS AND SYNTHETIC SEQUENCE PROCESSES
EP4638795A1 (en) 2022-12-22 2025-10-29 Illumina, Inc. Transition-metal catalyst compositions and methods for sequencing by synthesis
CN119095984A (zh) 2022-12-27 2024-12-06 伊路米纳有限公司 使用3'烯丙基封端的核苷酸的测序方法
CN116286720A (zh) * 2023-03-29 2023-06-23 赛纳生物科技(北京)有限公司 一种dna聚合酶
WO2024206407A2 (en) 2023-03-29 2024-10-03 Illumina, Inc. Naphthalimide dyes and uses in nucleic acid sequencing
CN119452101A (zh) 2023-03-30 2025-02-14 因美纳公司 用于核酸测序的组合物和方法
WO2024249200A1 (en) 2023-05-26 2024-12-05 Illumina, Inc. Methods for preserving methylation status during clustering
WO2024249591A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Methods for double-stranded sequencing by synthesis
WO2024249061A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Detecting a methylated nucleotide using a quencher
US20250075269A1 (en) 2023-08-31 2025-03-06 Illumina, Inc. Compositions and methods for nucleic acid sequencing
US20250137048A1 (en) 2023-10-26 2025-05-01 Illumina, Inc. 4,5-substituted naphthalimide dyes and uses in nucleic acid sequencing
WO2025106715A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Conjugated polymers or polymer dots as fluorescent labels
WO2025136890A1 (en) 2023-12-18 2025-06-26 Illumina, Inc. Hydrogel nanoparticles as labeling scaffold in sequencing
US20250215491A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Sequencing with single-stranded binding proteins
US20250215493A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing
WO2025144711A1 (en) 2023-12-29 2025-07-03 Illumina, Inc. Tricyclic polymethine dyes for nucleic acid sequencing
WO2025184226A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. Nucleotides with terminal phosphate capping
WO2025188906A1 (en) 2024-03-08 2025-09-12 Illumina, Inc. Modified adenosine nucleotides
WO2025230914A1 (en) 2024-04-29 2025-11-06 Illumina, Inc. Nucleotides with enzyme-triggered self-immolative linkers for sequencing by synthesis
WO2025264831A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2025264836A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2026006314A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Tagging target regions prior to nucleotide sequencing
WO2026035914A1 (en) 2024-08-09 2026-02-12 Illumina, Inc. Nucleotides having 3'-hydroxy blocking groups

Family Cites Families (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA258147A (en) 1926-02-16 W. Pease John Apple paring machine
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US5756334A (en) 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
EP0910664A1 (en) 1996-04-15 1999-04-28 University Of Southern California Synthesis of fluorophore-labeled dna
EP0822256B1 (en) 1996-07-29 2003-09-24 Toyo Boseki Kabushiki Kaisha Modified thermostable DNA polymerase, and DNA polymerase composition for nucleic acid amplification
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US5882904A (en) 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants
US5948666A (en) 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases
US20050181440A1 (en) 1999-04-20 2005-08-18 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
WO2000068422A2 (en) 1999-05-07 2000-11-16 Roche Diagnostics Gmbh High density labeling of dna with modified or 'chromophore' carrying nucleotides and dna polymerases used
US6492161B1 (en) 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
EP1218546A4 (en) 1999-09-30 2005-01-05 New England Biolabs Inc INCORPORATION OF MODIFIED NUCLEOTIDES BY ARCHAEON POLYMERASE DNA AND ASSOCIATED METHODS
US8268605B2 (en) 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
US20030228616A1 (en) 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
JP4949585B2 (ja) 1999-10-29 2012-06-13 アジレント・テクノロジーズ・インク Dnaポリメラーゼを使用した組成物および方法
WO2001038546A1 (en) 1999-11-23 2001-05-31 Amersham Biosciences Corp Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
AU2007201277B2 (en) 2000-05-24 2011-10-13 Third Wave Technologies, Inc. Detection of RNA
WO2002004680A2 (en) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
EP1373889A2 (en) 2000-07-31 2004-01-02 Maxygen, Inc. Biosensors, reagents and diagnostic applications of directed evolution
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
EP1395805A4 (en) 2001-06-11 2005-03-09 Illumina Inc MULTIPLEXED DETECTION TECHNIQUES
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
GB0509508D0 (en) * 2005-05-10 2005-06-15 Solexa Ltd Improved polymerases
WO2006120433A1 (en) 2005-05-10 2006-11-16 Solexa Limited Improved polymerases
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US8921086B2 (en) 2005-12-22 2014-12-30 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
DK2044188T3 (en) 2006-07-14 2015-12-14 Novozymes As Polypeptides with lipase activity and polynucleotides encoding them
WO2008029085A2 (en) 2006-09-06 2008-03-13 Medical Research Council Polymerase
GB0617575D0 (en) 2006-09-06 2006-10-18 Syngenta Ltd Herbicidal compounds and compositions
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US7948015B2 (en) 2006-12-14 2011-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
JP5386367B2 (ja) 2006-12-29 2014-01-15 アジレント・テクノロジーズ・インク 分断ポリメラーゼを使用する組成物および方法
JP5357893B2 (ja) 2007-12-27 2013-12-04 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 迅速超長鎖pcrのための単一酵素系
WO2009131919A2 (en) 2008-04-22 2009-10-29 New England Biolabs, Inc. Polymerases for incorporating modified nucleotides
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8481685B2 (en) 2008-11-03 2013-07-09 Kapa Biosystems Modified DNA polymerases
WO2010062776A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
AU2010205133B2 (en) 2009-01-15 2015-01-22 Hokkaido Mitsui Chemicals Inc. Enzyme preparation containing thermostable dna polymerase, method for producing same, and method for detecting subject organism to be detected
WO2011026194A1 (en) 2009-09-07 2011-03-10 Reproductive Health Science Pty Ltd Nucleic acid extraction
US8965076B2 (en) 2010-01-13 2015-02-24 Illumina, Inc. Data processing system and methods
US20110301041A1 (en) 2010-02-26 2011-12-08 Life Technologies Corporation Modified Proteins and Methods of Making and Using Same
GB201007384D0 (en) 2010-04-30 2010-06-16 Medical Res Council Enzymes
EP2632593B1 (en) 2010-10-27 2021-09-29 Illumina, Inc. Flow cells for biological or chemical analysis
WO2012154934A1 (en) 2011-05-11 2012-11-15 New England Biolabs, Inc. Dna polymerase variants with reduced exonuclease activity and uses thereof
US8921044B2 (en) 2011-05-11 2014-12-30 New England Biolabs, Inc. DNA polymerase variants with reduced exonuclease activity and uses thereof
JP6478444B2 (ja) * 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性dnaポリメラーゼ
US10421996B2 (en) 2013-03-14 2019-09-24 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
BR112015022448B1 (pt) 2013-03-15 2020-12-08 Illumina Cambridge Limited molécula de nucleotídeo ou nucleosídeo modificado, métodos para preparar o crescimento de polinucleotídeo complementar a polinucleotídeo alvo de fita simples em reação de sequenciação e para determinar a sequência de polinucleotídeo alvo de fita simples e kit
GB2526261B (en) 2014-04-28 2017-08-02 Gelliner Ltd Encoded cells and cell arrays
EP3702471B1 (en) * 2014-06-27 2025-12-24 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
JP6594955B2 (ja) * 2014-08-27 2019-10-23 ニユー・イングランド・バイオレイブス・インコーポレイテツド シントン形成
WO2016054096A1 (en) 2014-09-30 2016-04-07 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
CN107922929A (zh) 2015-09-09 2018-04-17 凯杰有限公司 聚合酶
TWI658139B (zh) * 2016-11-01 2019-05-01 體學生物科技股份有限公司 用以提升核苷酸類似物併入之重組dna聚合酶
WO2018126470A1 (zh) 2017-01-09 2018-07-12 深圳华大智造科技有限公司 重组dna聚合酶
US20200002689A1 (en) 2017-02-13 2020-01-02 Qiagen Sciences, Llc Polymerase enzyme from 9°n
WO2018148727A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from 9°n
WO2018148723A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus abyssi
WO2018148724A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus furiosus
WO2018148726A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from phage t4
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
CN117802065A (zh) 2018-09-03 2024-04-02 深圳华大生命科学研究院 聚合活性提高的重组型kod聚合酶
WO2020056044A1 (en) 2018-09-11 2020-03-19 Singular Genomics Systems, Inc. Modified archaeal family b polymerases
EP3853358A1 (en) 2018-09-17 2021-07-28 Omniome, Inc. Engineered polymerases for improved sequencing
US11104888B2 (en) 2018-10-31 2021-08-31 Illumina, Inc. Polymerases, compositions, and methods of use
EP3891275A2 (en) 2018-12-05 2021-10-13 Illumina, Inc. Polymerases, compositions, and methods of use
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group

Similar Documents

Publication Publication Date Title
JP2022511206A5 (https=)
JPWO2020117968A5 (https=)
JP2025120176A5 (https=)
JP2019536425A5 (https=)
JPWO2020092830A5 (https=)
JPWO2020139783A5 (https=)
CN112746063B (zh) 核苷转移酶的新功能及应用
US12168785B2 (en) Recombinant KOD polymerase
CA3064828A1 (en) Bipartite base editor (bbe) architectures and type-ii-c-cas9 zinc finger editing
JP2008538286A5 (https=)
CN109937252B (zh) 重组dna聚合酶
CN112725301B (zh) Taq DNA聚合酶突变体及其应用
JP2008539750A5 (https=)
KAGAWA et al. Stable structure of thermophilic proton ATPase beta subunit
Argos et al. Primary structural relationships may reflect similar DNA replication strategies
WO2023143123A1 (zh) 可控合成单链dna的末端转移酶变体及应用
CN108795900B (zh) Dna聚合酶及其制备方法
JP2021522825A5 (https=)
JP2004533811A5 (https=)
CN117396600A (zh) 耐热b家族dna聚合酶突变体及其应用
CN110747191A (zh) 多肽、嵌合聚合酶及其应用
JP2020127419A5 (https=)
WO2000066722B1 (en) Collection of prokaryotic dna for two hybrid systems helicobacter pylori protein-protein interactions and application thereof
CN116004564A (zh) 逆转录突变体及其应用
JPWO2020219908A5 (https=)