JP2021530671A - 正確な測色に基づく試験片リーダ・システム - Google Patents
正確な測色に基づく試験片リーダ・システム Download PDFInfo
- Publication number
- JP2021530671A JP2021530671A JP2020564547A JP2020564547A JP2021530671A JP 2021530671 A JP2021530671 A JP 2021530671A JP 2020564547 A JP2020564547 A JP 2020564547A JP 2020564547 A JP2020564547 A JP 2020564547A JP 2021530671 A JP2021530671 A JP 2021530671A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- color
- digital image
- specimen
- pad
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005259 measurement Methods 0.000 title description 18
- 238000012360 testing method Methods 0.000 claims abstract description 93
- 238000000034 method Methods 0.000 claims abstract description 68
- 239000000126 substance Substances 0.000 claims abstract description 35
- 230000002452 interceptive effect Effects 0.000 claims abstract description 32
- 238000011088 calibration curve Methods 0.000 claims abstract description 31
- 238000001228 spectrum Methods 0.000 claims abstract description 13
- 238000012937 correction Methods 0.000 claims abstract description 12
- 239000000523 sample Substances 0.000 claims description 119
- 238000005286 illumination Methods 0.000 claims description 21
- 230000008859 change Effects 0.000 claims description 19
- 239000012488 sample solution Substances 0.000 claims description 18
- 238000010606 normalization Methods 0.000 claims description 10
- 239000003086 colorant Substances 0.000 claims description 7
- 238000004458 analytical method Methods 0.000 abstract description 31
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 23
- 238000010586 diagram Methods 0.000 description 16
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 12
- 230000006870 function Effects 0.000 description 11
- 230000005540 biological transmission Effects 0.000 description 9
- 238000001514 detection method Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 230000035945 sensitivity Effects 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 238000003384 imaging method Methods 0.000 description 7
- 229940109239 creatinine Drugs 0.000 description 6
- 238000010801 machine learning Methods 0.000 description 6
- OBHRVMZSZIDDEK-UHFFFAOYSA-N urobilinogen Chemical compound CCC1=C(C)C(=O)NC1CC1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(CC3C(=C(CC)C(=O)N3)C)N2)CCC(O)=O)N1 OBHRVMZSZIDDEK-UHFFFAOYSA-N 0.000 description 6
- 239000008280 blood Substances 0.000 description 4
- 238000004590 computer program Methods 0.000 description 4
- 230000031700 light absorption Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 210000002700 urine Anatomy 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000001427 coherent effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000003909 pattern recognition Methods 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 125000002066 L-histidyl group Chemical group [H]N1C([H])=NC(C([H])([H])[C@](C(=O)[*])([H])N([H])[H])=C1[H] 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 238000001739 density measurement Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 150000002826 nitrites Chemical class 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
- 238000002562 urinalysis Methods 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/80—Indicating pH value
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/79—Photometric titration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/90—Determination of colour characteristics
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V10/00—Arrangements for image or video recognition or understanding
- G06V10/40—Extraction of image or video features
- G06V10/50—Extraction of image or video features by performing operations within image blocks; by using histograms, e.g. histogram of oriented gradients [HoG]; by summing image-intensity values; Projection analysis
- G06V10/507—Summing image-intensity values; Histogram projection analysis
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V20/00—Scenes; Scene-specific elements
- G06V20/10—Terrestrial scenes
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/56—Cameras or camera modules comprising electronic image sensors; Control thereof provided with illuminating means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N2021/752—Devices comprising reaction zones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N2021/757—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated using immobilised reagents
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
- G01N2021/8488—Investigating reagent band the band presenting reference patches
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/02—Mechanical
- G01N2201/022—Casings
- G01N2201/0221—Portable; cableless; compact; hand-held
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
- G01N2201/0627—Use of several LED's for spectral resolution
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/127—Calibration; base line adjustment; drift compensation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/127—Calibration; base line adjustment; drift compensation
- G01N2201/12746—Calibration values determination
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/127—Calibration; base line adjustment; drift compensation
- G01N2201/12746—Calibration values determination
- G01N2201/12761—Precalibration, e.g. for a given series of reagents
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
- G06T2207/10141—Special mode during image acquisition
- G06T2207/10152—Varying illumination
Landscapes
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Theoretical Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Multimedia (AREA)
- Molecular Biology (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Signal Processing (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Spectrometry And Color Measurement (AREA)
Abstract
Description
Claims (23)
- 方法であって、
試料で湿った試験片を光の選択スペクトルで照明することであって、前記試験片が、前記試料内に検体が存在するときに色が変化するように構成される試験パッドを含む、前記照明することと、
前記試験片の少なくとも1つのデジタル画像を取得することと、
色強度に影響を与える前記試料内の1つまたは複数の干渉物質についての補正がなされた、前記試料における検体濃度を決定するために、校正曲線に対して前記少なくとも1つのデジタル画像から前記色強度を分析することと、
を含む、方法。 - 前記照明することが、
前記試験パッドの異なるものについて照明の色を変更することをさらに含む、請求項1に記載の方法。 - 前記少なくとも1つのデジタル画像が、静止デジタル画像またはビデオ・デジタル画像を含む、請求項1に記載の方法。
- 前記少なくとも1つのデジタル画像が、タイムスタンプ付与され、前記方法が、
前記試験片が前記試料で湿っているときに計時することをさらに含む、請求項1に記載の方法。 - 前記少なくとも1つのデジタル画像が、モバイル・デバイスのコンポーネントであるデジタル・カメラを用いて取得される、請求項1に記載の方法。
- 前記モバイル・デバイスが、スマートフォンまたはタブレットである、請求項5に記載の方法。
- 校正パッドの少なくとも1つのデジタル画像を取得することであって、前記校正パッドが、既知の色度の色系列スケールを構成する色基準パッチを有する、前記取得することと、
i)前記校正パッドの前記少なくとも1つのデジタル画像をその成分の赤、緑、および青(RGB)チャネルに分離すること、ならびにii)前記RGBチャネルのそれぞれの強度を測定することによって、前記校正パッドの前記少なくとも1つのデジタル画像から前記色基準パッチの色を分析することと、
前記色基準パッチの前記既知の色度に対する前記RGBチャネルのそれぞれの前記強度のプロットを取得することと、
前記プロットを記憶することと、
をさらに含む、請求項1に記載の方法。 - 前記校正パッドの少なくとも1つのデジタル画像、および様々な検体濃度において既知のpHを有する一連の検体溶液で湿った試験片の少なくとも1つのデジタル画像を取得することと、
前記プロットから判断される正規化係数を用いて、前記試験片の前記少なくとも1つのデジタル画像から前記色強度を調整することと、
前記校正曲線を形成するために、前記様々な検体濃度に対して前記試験片の前記少なくとも1つのデジタル画像から前記色強度をプロットすることと、
によって、前記校正曲線を生成することをさらに含む、請求項7に記載の方法。 - pH値の範囲についての前記校正曲線を取得するために、前記様々な検体濃度において異なる既知のpHを有する追加の一連の検体溶液で前記校正曲線を前記生成することをx回繰り返すことをさらに含む、請求項8に記載の方法。
- 前記干渉物質についての前記校正曲線を取得するために、追加された干渉物質を有する前記一連の検体溶液で前記校正曲線を前記生成することをy回繰り返すことをさらに含む、請求項8に記載の方法。
- 前記干渉物質が、前記一連の検体溶液に個別に追加される、請求項10に記載の方法。
- 2つ以上の前記干渉物質の組み合わせが、前記一連の検体溶液に追加される、請求項10に記載の方法。
- 前記照明することが、
異なる色で光を放出する複数の発光ダイオード(LED)を用いて異なる色の照明を提供することを含む、請求項1に記載の方法。 - 方法であって、
校正パッドの少なくとも1つのデジタル画像、および様々な検体濃度において既知のpHを有する一連の検体溶液で湿った試験片の少なくとも1つのデジタル画像を取得することであって、前記校正パッドが、既知の色度の色系列スケールを構成する色基準パッチを有する、前記取得することと、
校正曲線を形成するために、前記様々な検体濃度に対して前記試験片の前記少なくとも1つのデジタル画像から前記色強度をプロットすることと、
によって、前記校正曲線を生成することを含む、方法。 - pH値の範囲についての前記校正曲線を取得するために、前記様々な検体濃度において異なる既知のpHを有する追加の一連の検体溶液で前記校正曲線を前記生成することをx回繰り返すことをさらに含む、請求項14に記載の方法。
- 干渉物質についての前記校正曲線を取得するために、追加された前記干渉物質を有する前記一連の検体溶液で前記校正曲線を前記生成することをy回繰り返すことをさらに含む、請求項15に記載の方法。
- 試料で湿った試験片を光の選択スペクトルで照明することであって、前記試験片が、前記試料内に検体が存在するときに色が変化するように構成される試験パッドを含む、前記照明することと、
前記試験片の少なくとも1つのデジタル画像を取得することと、
色強度に影響を与える前記試料内の1つまたは複数の干渉物質についての補正がなされた、前記試料における検体濃度を決定するために、前記校正曲線に対して前記少なくとも1つのデジタル画像から前記色強度を分析することと、
をさらに含む、請求項14に記載の方法。 - 前記照明することが、
前記試験パッドの異なるものについて照明の色を変更することをさらに含む、請求項17に記載の方法。 - デバイスであって、
メモリに接続されたプロセッサであって、
試料で湿った試験片を光の選択スペクトルで照明することであって、前記試験片が、前記試料内に検体が存在するときに色が変化するように構成される試験パッドを含む、前記照明することと、
前記試験片の少なくとも1つのデジタル画像を取得することと、
色強度に影響を与える前記試料内の1つまたは複数の干渉物質についての補正がなされた、前記試料における検体濃度を決定するために、校正曲線に対して前記少なくとも1つのデジタル画像から前記色強度を分析することと、
を実行するように構成される、前記プロセッサを含む、デバイス。 - 前記プロセッサが、
校正パッドの少なくとも1つのデジタル画像を取得することであって、前記校正パッドが、既知の色度の色系列スケールを構成する色基準パッチを有する、前記取得することと、
i)前記校正パッドの前記少なくとも1つのデジタル画像をその成分の赤、緑、および青(RGB)チャネルに分離すること、ならびにii)前記RGBチャネルのそれぞれの強度を測定することによって、前記校正パッドの前記少なくとも1つのデジタル画像から前記色基準パッチの色を分析することと、
前記色基準パッチの前記既知の色度に対する前記RGBチャネルのそれぞれの前記強度のプロットを取得することと、
前記プロットを記憶することと、
を実行するようにさらに構成される、請求項19に記載のデバイス。 - 前記少なくとも1つのデジタル画像が、モバイル・デバイスのコンポーネントであるデジタル・カメラを用いて取得される、請求項19に記載のデバイス。
- 前記モバイル・デバイスが、スマートフォンまたはタブレットである、請求項21に記載のデバイス。
- 前記プロセッサが、前記照明することを実行するときに、
異なる色で光を放出する複数のLEDを用いて異なる色の照明を提供することを実行するようにさらに構成される、請求項19に記載のデバイス。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16/022,282 | 2018-06-28 | ||
US16/022,282 US10605741B2 (en) | 2018-06-28 | 2018-06-28 | Accurate colorimetric based test strip reader system |
PCT/IB2019/054088 WO2020003021A1 (en) | 2018-06-28 | 2019-05-17 | Accurate colorimetric based test strip reader system |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2021530671A true JP2021530671A (ja) | 2021-11-11 |
JP7250823B2 JP7250823B2 (ja) | 2023-04-03 |
Family
ID=68986140
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2020564547A Active JP7250823B2 (ja) | 2018-06-28 | 2019-05-17 | 正確な測色に基づく試験片リーダ・システム |
Country Status (6)
Country | Link |
---|---|
US (2) | US10605741B2 (ja) |
JP (1) | JP7250823B2 (ja) |
CN (1) | CN112074725B (ja) |
DE (1) | DE112019001825B4 (ja) |
GB (1) | GB2586939B (ja) |
WO (1) | WO2020003021A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2024053464A1 (ja) * | 2022-09-06 | 2024-03-14 | 日東電工株式会社 | 分析システム、分析方法 |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11016030B2 (en) * | 2017-10-26 | 2021-05-25 | Delta Electronics, Inc. | Detection method and detection device with compensation function |
US20200209214A1 (en) * | 2019-01-02 | 2020-07-02 | Healthy.Io Ltd. | Urinalysis testing kit with encoded data |
US11733170B2 (en) * | 2019-08-07 | 2023-08-22 | International Business Machines Corporation | Optical sensor system for quantitative colorimetric liquid analysis |
CN116157867A (zh) * | 2020-07-08 | 2023-05-23 | Exa健康公司 | Lfa测试条的神经网络分析 |
GB2597946A (en) * | 2020-08-11 | 2022-02-16 | Adey Holdings 2008 Ltd | Testing of central heating system water |
CA3192942A1 (en) | 2020-09-17 | 2022-03-24 | Scanwell Health, Inc. | Diagnostic test kits and methods of analyzing the same |
CN112133436B (zh) * | 2020-10-13 | 2021-06-29 | 广州万孚健康科技有限公司 | 一种基于大数据分析的健康警示方法、系统和可读存储介质 |
CA3198824A1 (en) | 2020-10-23 | 2022-04-28 | Becton, Dickinson And Company | Systems and methods for imaging and image-based analysis of test devices |
USD970033S1 (en) | 2020-10-23 | 2022-11-15 | Becton, Dickinson And Company | Cartridge imaging background device |
CN113866400B (zh) * | 2021-10-09 | 2023-09-26 | 武汉纳达康生物科技有限公司 | 快速毒检方法、一分钟快速毒检装置及分布式毒检系统 |
CN114002217B (zh) * | 2021-11-02 | 2024-05-14 | 苏州国科医工科技发展(集团)有限公司 | 一种通过ph试纸图像读取ph值的方法 |
JP2024060999A (ja) | 2022-10-20 | 2024-05-07 | アークレイ株式会社 | 測定システム及び測定方法 |
Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07190940A (ja) * | 1993-09-07 | 1995-07-28 | Miles Inc | ビデオ試験片読取り装置及び試験片の評価方法 |
JP2000338106A (ja) * | 1999-05-28 | 2000-12-08 | Sanko Junyaku Kk | 免疫反応による発色画像の処理定量方法 |
JP2001337087A (ja) * | 2000-05-26 | 2001-12-07 | Dev Center For Biotechnol | 尿酸アッセイに用いるための干渉除去膜、テストストリップ、キット及び方法 |
JP2009236487A (ja) * | 2008-03-25 | 2009-10-15 | Terumo Corp | ヘマトクリット値または血液成分濃度の測定方法および測定装置 |
JP2010518397A (ja) * | 2007-02-06 | 2010-05-27 | グルメトリクス, インコーポレイテッド | pH及びグルコースの光学的決定 |
JP2013101102A (ja) * | 2011-10-17 | 2013-05-23 | Arkray Inc | 蛋白質濃度評価方法、分析用具、及び分析装置 |
JP2014238263A (ja) * | 2011-09-30 | 2014-12-18 | テルモ株式会社 | 血液成分分析装置 |
JP2015509582A (ja) * | 2012-02-03 | 2015-03-30 | ユニバーシティ・オブ・シンシナティ | 比色アッセイを解析するための方法、システム、および装置 |
JP2015520389A (ja) * | 2012-06-22 | 2015-07-16 | エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト | 体液中の分析物を検出する方法および装置 |
JP2016514830A (ja) * | 2013-03-19 | 2016-05-23 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 体液試料中の分析物濃度の補正値を生成するための方法/装置 |
JP2017215337A (ja) * | 2011-03-31 | 2017-12-07 | ノバラム ディーエックス リミテッド | ソフトウェアアプリケーションおよびプロセッサ |
Family Cites Families (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5760913A (en) | 1996-02-12 | 1998-06-02 | Splash Technology, Inc. | Color calibration method and system having independent color scanner profiles |
GB9700729D0 (en) | 1997-01-15 | 1997-03-05 | Axis Biochemicals As | System |
US6394952B1 (en) | 1998-02-03 | 2002-05-28 | Adeza Biomedical Corporation | Point of care diagnostic systems |
HUP9800290A3 (en) | 1998-02-11 | 2000-03-28 | 77 Elektronika Mueszeripari Kf | Device for automatically analyzing a test stripe |
US7577469B1 (en) * | 1999-03-11 | 2009-08-18 | Jack L. Aronowitz | Noninvasive transdermal systems for detecting an analyte in a biological fluid and methods |
CN102099816A (zh) | 2008-07-17 | 2011-06-15 | 环球生物医疗感测器私人有限公司 | 利用彩色编码栏的自动信息传送 |
DE102008058132A1 (de) * | 2008-11-14 | 2010-05-20 | opTricon GmbH Entwicklungsesellschaft für optische Technologien | Gerät und Verfahren zur Auswertung und Bewertung eines Teststreifens |
CN104215757B (zh) | 2010-05-28 | 2018-12-04 | 李金波 | 生物体液样本定量检测装置及其检测方法 |
US8655009B2 (en) | 2010-09-15 | 2014-02-18 | Stephen L. Chen | Method and apparatus for performing color-based reaction testing of biological materials |
US8506901B2 (en) | 2010-11-03 | 2013-08-13 | Teco Diagnostics | All-in-one specimen cup with optically readable results |
US20120188378A1 (en) | 2011-01-20 | 2012-07-26 | Middleland Sensing Technology Inc. | Automatic analyzing method and system for test strips |
CN102645431A (zh) * | 2011-02-18 | 2012-08-22 | 三浦工业株式会社 | 亚硝酸根离子的定量方法 |
GB2496104B (en) | 2011-10-25 | 2019-12-11 | Alere Switzerland Gmbh | Improvements in or relating to reading of assays |
US8983181B2 (en) | 2011-11-28 | 2015-03-17 | Psiflow Technology Inc. | Method and system for determining the color of an object in a photo |
KR102005597B1 (ko) | 2012-02-06 | 2019-07-30 | 더 리전트 오브 더 유니버시티 오브 캘리포니아 | 휴대용 신속 진단 테스트 리더기 |
US9063091B2 (en) * | 2012-04-06 | 2015-06-23 | Ixensor Inc. | Test strips and method for reading test strips |
US10132802B2 (en) | 2012-04-17 | 2018-11-20 | i-calQ, LLC | Device for performing a diagnostic test and methods for use thereof |
EP2657681A1 (en) * | 2012-04-26 | 2013-10-30 | Roche Diagnostics GmbH | Improvement of the sensitivity and the dynamic range of photometric assays by generating multiple calibration curves |
WO2014011786A2 (en) | 2012-07-10 | 2014-01-16 | Fgroupip1, Llc | Method to identify chemical compounds using colorimetric spot tests |
CN104969068B (zh) * | 2012-08-08 | 2019-06-18 | 思勘度股份有限公司 | 用于在自动校准环境中执行及量化由特定浓度的生物分析物诱发的色彩改变的方法及设备 |
US9528941B2 (en) * | 2012-08-08 | 2016-12-27 | Scanadu Incorporated | Method and apparatus for determining analyte concentration by quantifying and interpreting color information captured in a continuous or periodic manner |
US9285323B2 (en) | 2012-08-08 | 2016-03-15 | Scanadu Incorporated | Quantifying color changes of chemical test pads induced concentrations of biological analytes under different lighting conditions |
US9778200B2 (en) * | 2012-12-18 | 2017-10-03 | Ixensor Co., Ltd. | Method and apparatus for analyte measurement |
US9787815B2 (en) * | 2013-01-21 | 2017-10-10 | Cornell University | Smartphone-based apparatus and method |
FI20135189L (fi) | 2013-02-28 | 2014-08-29 | iStoc Oy | Kannettava lateraalinen koetulosten lukija |
US9488657B2 (en) | 2013-03-15 | 2016-11-08 | Charm Sciences, Inc. | Detection sensor systems and methods |
WO2015038717A1 (en) | 2013-09-11 | 2015-03-19 | Wellumina Health, Inc. | System for diagnostic testing of liquid samples |
US9569858B2 (en) | 2014-02-13 | 2017-02-14 | James Bennett Babcock | Cloud-based system for water analysis |
CN106068449A (zh) * | 2014-03-05 | 2016-11-02 | 思勘度股份有限公司 | 通过量化和解释颜色的分析物浓度 |
US9933359B2 (en) * | 2014-06-23 | 2018-04-03 | Xerox Corporation | Vendor exclusivity security feature for paper-based diagnostic solution |
US9863811B2 (en) | 2014-08-15 | 2018-01-09 | Scanadu Incorporated | Precision luxmeter methods for digital cameras to quantify colors in uncontrolled lighting environments |
US9568432B2 (en) | 2014-09-26 | 2017-02-14 | Intel Corporation | Sensor apparatus to determine a value of a chemical parameter based on a color shade and methods thereof |
US9983139B2 (en) | 2014-11-10 | 2018-05-29 | Donald Channing Cooper | Modular illumination and sensor chamber |
US20160139156A1 (en) | 2014-11-18 | 2016-05-19 | Welltwigs LLC | Apparatuses, methods, and systems for home monitoring of physiological states and conditions |
WO2016132243A1 (en) | 2015-02-19 | 2016-08-25 | Renalyx Health Systems Pvt Ltd | Method and device for performing colorimetric analysis of a test fluid to evaluate physiological parameters |
CN104964973A (zh) | 2015-07-08 | 2015-10-07 | 邓双胜 | 一种基于移动终端摄像头的试纸读取与分析方法与系统 |
US20170026620A1 (en) | 2015-07-24 | 2017-01-26 | Venkatasubramaniam Kalambur | Apparatus and a method to determine the concentration of an analyte |
US10354412B2 (en) | 2015-11-30 | 2019-07-16 | Detectachem, Inc. | Receptacle for detection of targeted substances |
US20170184506A1 (en) | 2015-12-29 | 2017-06-29 | Pritesh Arjunbhai Patel | Reagent test strips comprising reference regions for measurement with colorimetric test platform |
CN105974120B (zh) | 2016-04-27 | 2017-11-14 | 北方工业大学 | 一种c反应蛋白色度自动检测装置与方法 |
US9958665B2 (en) * | 2016-05-11 | 2018-05-01 | Bonraybio Co., Ltd. | Testing equipment with magnifying function |
US10346710B2 (en) | 2016-09-29 | 2019-07-09 | Datacolor Inc. | Multi-agent training of a color identification neural network |
US11802868B2 (en) * | 2016-10-17 | 2023-10-31 | Reliant Immune Diagnostics, Inc. | System and method for variable function mobile application for providing medical test results |
EP3335638A1 (en) | 2016-12-13 | 2018-06-20 | Colorimetrix GmbH | Method and device for estimation of ovulation date |
EP3339850B1 (en) * | 2016-12-21 | 2019-08-07 | Roche Diagnostics GmbH | Method and device for determining a concentration of at least one analyte |
CN107266351A (zh) * | 2017-06-26 | 2017-10-20 | 成都理工大学 | 基于结晶紫酰肼的亚硝酸盐含量测定 |
US10656405B2 (en) * | 2018-01-20 | 2020-05-19 | International Business Machines Corporation | Color image capture under controlled environment for mobile devices |
DE102018002850B3 (de) | 2018-04-10 | 2019-08-14 | Robert Simmoteit | Analyseverfahren und Anschmutzung |
US20210208081A1 (en) | 2018-05-15 | 2021-07-08 | Vivosens Inc | Analysis of urine test strips with mobile camera analysys and providing recommendation by customising data |
-
2018
- 2018-06-28 US US16/022,282 patent/US10605741B2/en not_active Expired - Fee Related
-
2019
- 2019-05-17 GB GB2100095.5A patent/GB2586939B/en active Active
- 2019-05-17 WO PCT/IB2019/054088 patent/WO2020003021A1/en active Application Filing
- 2019-05-17 DE DE112019001825.6T patent/DE112019001825B4/de active Active
- 2019-05-17 JP JP2020564547A patent/JP7250823B2/ja active Active
- 2019-05-17 CN CN201980030334.4A patent/CN112074725B/zh active Active
-
2020
- 2020-02-20 US US16/796,763 patent/US11307147B2/en active Active
Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07190940A (ja) * | 1993-09-07 | 1995-07-28 | Miles Inc | ビデオ試験片読取り装置及び試験片の評価方法 |
JP2000338106A (ja) * | 1999-05-28 | 2000-12-08 | Sanko Junyaku Kk | 免疫反応による発色画像の処理定量方法 |
JP2001337087A (ja) * | 2000-05-26 | 2001-12-07 | Dev Center For Biotechnol | 尿酸アッセイに用いるための干渉除去膜、テストストリップ、キット及び方法 |
JP2010518397A (ja) * | 2007-02-06 | 2010-05-27 | グルメトリクス, インコーポレイテッド | pH及びグルコースの光学的決定 |
JP2009236487A (ja) * | 2008-03-25 | 2009-10-15 | Terumo Corp | ヘマトクリット値または血液成分濃度の測定方法および測定装置 |
JP2017215337A (ja) * | 2011-03-31 | 2017-12-07 | ノバラム ディーエックス リミテッド | ソフトウェアアプリケーションおよびプロセッサ |
JP2014238263A (ja) * | 2011-09-30 | 2014-12-18 | テルモ株式会社 | 血液成分分析装置 |
JP2013101102A (ja) * | 2011-10-17 | 2013-05-23 | Arkray Inc | 蛋白質濃度評価方法、分析用具、及び分析装置 |
JP2015509582A (ja) * | 2012-02-03 | 2015-03-30 | ユニバーシティ・オブ・シンシナティ | 比色アッセイを解析するための方法、システム、および装置 |
JP2015520389A (ja) * | 2012-06-22 | 2015-07-16 | エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト | 体液中の分析物を検出する方法および装置 |
JP2016514830A (ja) * | 2013-03-19 | 2016-05-23 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 体液試料中の分析物濃度の補正値を生成するための方法/装置 |
Non-Patent Citations (2)
Title |
---|
ANURADHA SONI AND SANDEEP KUMAR JHA: "Smartphone based non-invasive salivary glucose biosensor", ANALYTICA CHIMICA ACTA, vol. 996, JPN6022041955, 17 October 2017 (2017-10-17), pages 54 - 63, ISSN: 0005006689 * |
PINKY A. BAUTISTA ET AL.: "Color standardization in whole slide imaging using a color calibration slide", J PATHOL INFORM, vol. 5, JPN6022041956, 31 January 2014 (2014-01-31), pages 1 - 11, ISSN: 0005006690 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2024053464A1 (ja) * | 2022-09-06 | 2024-03-14 | 日東電工株式会社 | 分析システム、分析方法 |
Also Published As
Publication number | Publication date |
---|---|
WO2020003021A1 (en) | 2020-01-02 |
DE112019001825T5 (de) | 2020-12-24 |
US20200003698A1 (en) | 2020-01-02 |
CN112074725B (zh) | 2024-04-05 |
JP7250823B2 (ja) | 2023-04-03 |
GB202100095D0 (en) | 2021-02-17 |
GB2586939A (en) | 2021-03-10 |
US20200191722A1 (en) | 2020-06-18 |
DE112019001825B4 (de) | 2022-02-03 |
US11307147B2 (en) | 2022-04-19 |
GB2586939B (en) | 2021-06-30 |
CN112074725A (zh) | 2020-12-11 |
US10605741B2 (en) | 2020-03-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP7250823B2 (ja) | 正確な測色に基づく試験片リーダ・システム | |
US11709096B2 (en) | Precision luxmeter methods for digital cameras to quantify colors in uncontrolled lighting environments | |
US20210302321A1 (en) | Calibration method for reagent card analyzers | |
EP3612963B1 (en) | Biochemical analyser based on a machine learning algorithm using test strips and a smartdevice | |
JP6326574B2 (ja) | モバイル・デバイスを使用した可搬式の医療用診断システム及び方法 | |
US20210263018A1 (en) | Lateral flow test strip immunoassay in vitro diagnostic device | |
US11585804B2 (en) | Urinalysis device and test strip for home and point of care use | |
GB2483482A (en) | An optical testing system | |
KR20210028180A (ko) | 샘플에서 분석물을 검출하기 위한 검출 방법 | |
WO2015038717A1 (en) | System for diagnostic testing of liquid samples | |
TW201928879A (zh) | 基於顏色形成反應執行分析量測的方法及裝置 | |
WO2017019762A1 (en) | Image based photometry | |
CN112437202A (zh) | 数据的处理方法、处理装置、图像处理系统和处理器 | |
Kibria et al. | Smartphone-based point-of-care urinalysis assessment | |
RU120556U1 (ru) | Устройство для чтения результатов анализов, выполненных с помощью тест-полосок | |
raj Konnaiyan | Smartphone Based 3D Printed Colorimeter for Biomedical Applications | |
WO2013073983A1 (ru) | Устройство для чтения результатов анализов, выполненных с помощью тест-полосок |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20210510 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20210927 |
|
RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7424 Effective date: 20220502 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20220804 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20221006 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20221202 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20230307 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20230322 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 7250823 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |