JP2021513837A - 微小流体装置 - Google Patents
微小流体装置 Download PDFInfo
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- JP2021513837A JP2021513837A JP2020541502A JP2020541502A JP2021513837A JP 2021513837 A JP2021513837 A JP 2021513837A JP 2020541502 A JP2020541502 A JP 2020541502A JP 2020541502 A JP2020541502 A JP 2020541502A JP 2021513837 A JP2021513837 A JP 2021513837A
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Abstract
Description
本発明は、一般に、微小流体装置を対象とし、特に、混入粒子及び標的細胞を含む生体試料の分析に使用できるそのような微小流体装置を対象とする。
単細胞生物学での最近の進展により、同質遺伝子細胞は、同一の条件下で成長した場合にも、遺伝子発現及び挙動に大きな差異を示し得ることが明らかになった。それにより、経時的な表現型における細胞間の差異を特徴付けるために、新しい装置が必要とされる。そのような装置は、単一の細胞を培養及び監視する際に有効なツールであるために、特定の基準を満たす必要がある。例えば、これらの装置は、投入直後に表現型特性を監視できるように、細胞の投入が容易であるべきである。さらに、多くの異なる個々の細胞を並行して成長させて、細胞間の差異を特徴付け、かつ平均化による個々の細胞の特性評価における測定誤差を克服する必要がある。これらの装置は、例えば、系統依存的動態を監視するために、一定のかつ十分に制御された成長条件下で長期間細胞の培養を可能にするように設計されなければならない。装置が、新しい培養培地又は試験剤に応答して動態変化を監視するために培養条件を変更できるならば、さらに好ましい。例えば、並行して同質遺伝子細胞に対して異なる培養培地を試験するか、又は並行して異なる細胞株での培地変化に対する応答を監視することが有利であり得る。
Claims (13)
- 標的細胞を収容するように適合されたセルチャネル(20)を有する基板(10)と、
第1の流体ポート(31)と流体接続する第1の端部(32)を有する流入チャネル(30)であって、前記セルチャネル(20)のそれぞれの第1の端部(22)が、前記流入チャネル(30)と流体接続している、流入チャネル(30)と、
第3の流体ポート(41)と流体接続する流出チャネル(40)であって、前記セルチャネル(20)のそれぞれの第2の端部(24)が、前記流出チャネル(40)と流体接続しており、前記セルチャネル(20)が、前記標的細胞が前記それぞれの障害物(25)を通過して前記流出チャネル(40)に入ることを防ぐように設計されたそれぞれの障害物(25)を備え、
第1のフィルタポート(61)と流体接続する第1の端部(62)を有するフィルタチャネル(60)と、
前記標的細胞を収容するように適合されたプレフィルタチャネル(70)
を備えるプレフィルタ(50)であって、前記プレフィルタチャネル(70)のそれぞれの第1の端部(72)が、前記フィルタチャネル60と流体接続しかつ前記プレフィルタチャネル(70)のそれぞれの第2の端部(74)が、前記流入チャネル(30)と流体接続する、プレフィルタ(50)
により特徴づけられる、流出チャネル(40)、
を備える微小流体装置1。 - 前記フィルタチャネル(60)が第2のフィルタポート(65)と流体接続する第2の端部(64)を有することを特徴とする、請求項1に記載の微小流体装置。
- 前記フィルタチャネル(60)の前記第1の端部(62)と前記第2の端部(64)の間で前後に前記標的細胞を含む流体の流れを誘導するように構成された流量制御装置(100)により特徴づけられる、請求項2に記載の微小流体装置。
- 前記第1のフィルタポート(61)と前記第2のフィルタポート(65)の間で前記標的細胞を含む流体の循環流を誘導するように構成された流量制御装置(100)により特徴づけられる、請求項2又は3に記載の微小流体装置。
- 前記流入チャネル(30)から前記プレフィルタチャネル(70)を通り前記フィルタチャネル(60)内に流体流を誘導するように構成された流量制御装置(100)により特徴づけられる、請求項1〜4のいずれかに記載の微小流体装置。
- 前記流入チャネル(30)から前記プレフィルタチャネル(70)を通り前記フィルタチャネル(60)内にかつ前記流入チャネル(30)から前記セルチャネル(20)を通り前記流出チャネル(40)内に前記流体流を誘導するように構成された前記流量制御装置(100)により特徴づけられる、請求項5に記載の微小流体装置。
- 前記フィルタチャネル(60)が第1のフィルタチャネル(60)であり;
前記プレフィルタチャネル(70)が第1のプレフィルタチャネル(70)であり;
前記プレフィルタ(50)が、
第3のフィルタポート(81)と流体接続する第1の端部(82)を有する第2のフィルタチャネル(80)と、
前記標的細胞を収容するように適合された第2のプレフィルタチャネル(90)を備え、前記第2のプレフィルタチャネル(90)のそれぞれの第1の端部(92)が、前記第2のフィルタチャネル(80)と流体接続しかつ前記第2のプレフィルタチャネル(90)のそれぞれの第2の端部(94)が、前記流入チャネル(30)と流体接続し、
前記第1のプレフィルタチャネル(70)の前記それぞれの第2の端部(74)が前記第2のフィルタチャネル(80)と流体接続している、
ことを特徴とする、請求項1〜6のいずれかに記載の微小流体装置。 - 前記第1のプレフィルタチャネル(70)が、前記第2のプレフィルタチャネル(90)と比較してより大きい直径又はより大きい高さ及び/又は幅を有することを特徴とする、請求項7に記載の微小流体装置。
- 前記流入チャネル(30)から前記プレフィルタチャネル(70)を通り前記フィルタチャネル(60)内に流体流を誘導すること(S2)を含む、請求項1〜8のいずれかに記載の微小流体装置(1)を操作する方法。
- 前記流体流を誘導すること(S2)が、前記流入チャネル(30)から前記プレフィルタチャネル(70)を通り前記フィルタチャネル(60)内にかつ前記流入チャネル(30)から前記セルチャネル(20)を通り前記流出チャネル(40)内に流体流を誘導すること(S2)を含む、請求項9に記載の方法。
- 前記流体流を誘導すること(S2)が、前記流出チャネル(40)から前記セルチャネル(20)の少なくとも一部を通り前記流入チャネル(30)内に前記プレフィルタチャネル(70)を通り前記フィルタチャネル(60)内に流体流を誘導すること(S2)を含む、請求項9又は10に記載の方法。
- 前記フィルタチャネル(60)が、第2のフィルタポート(65)と流体接続する第2の端部(64)を有し;かつ
前記フィルタチャネル(60)の前記第1の端部(62)と前記第2の端部(64)の間で前後に前記標的細胞を含む流体の流れを誘導すること(S1)をさらに含む、請求項9〜11のいずれかに記載の方法。 - 前記フィルタチャネル(60)が、第2のフィルタポート(65)と流体接続する第2の端部(64)を有し;かつ、前記第1のフィルタポート(61)と前記第2のフィルタポート(65)の間で前記標的細胞を含む流体の循環流を誘導すること(S1)をさらに含む、請求項9〜12のいずれかに記載の方法。
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