JP2021003050A5 - - Google Patents
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- Publication number
- JP2021003050A5 JP2021003050A5 JP2019118981A JP2019118981A JP2021003050A5 JP 2021003050 A5 JP2021003050 A5 JP 2021003050A5 JP 2019118981 A JP2019118981 A JP 2019118981A JP 2019118981 A JP2019118981 A JP 2019118981A JP 2021003050 A5 JP2021003050 A5 JP 2021003050A5
- Authority
- JP
- Japan
- Prior art keywords
- dna
- types
- detected
- fluorescently labeled
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 claims 13
- 238000002844 melting Methods 0.000 claims 8
- 230000008018 melting Effects 0.000 claims 8
- 239000000523 sample Substances 0.000 claims 7
- 239000007850 fluorescent dye Substances 0.000 claims 3
- 230000003321 amplification Effects 0.000 claims 2
- 238000007847 digital PCR Methods 0.000 claims 2
- 238000010438 heat treatment Methods 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019118981A JP7245734B2 (ja) | 2019-06-26 | 2019-06-26 | デジタルｐｃｒの測定方法および測定装置 |
| US17/620,077 US20220364164A1 (en) | 2019-06-26 | 2020-05-27 | Digital pcr measurement method and measurement device |
| PCT/JP2020/020998 WO2020261858A1 (ja) | 2019-06-26 | 2020-05-27 | デジタルｐｃｒの測定方法および測定装置 |
| JP2023038011A JP7545511B2 (ja) | 2019-06-26 | 2023-03-10 | デジタルｐｃｒの測定方法および測定装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019118981A JP7245734B2 (ja) | 2019-06-26 | 2019-06-26 | デジタルｐｃｒの測定方法および測定装置 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023038011A Division JP7545511B2 (ja) | 2019-06-26 | 2023-03-10 | デジタルｐｃｒの測定方法および測定装置 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021003050A JP2021003050A (ja) | 2021-01-14 |
| JP2021003050A5 true JP2021003050A5 (https=) | 2022-03-15 |
| JP7245734B2 JP7245734B2 (ja) | 2023-03-24 |
Family
ID=74061209
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019118981A Active JP7245734B2 (ja) | 2019-06-26 | 2019-06-26 | デジタルｐｃｒの測定方法および測定装置 |
| JP2023038011A Active JP7545511B2 (ja) | 2019-06-26 | 2023-03-10 | デジタルｐｃｒの測定方法および測定装置 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023038011A Active JP7545511B2 (ja) | 2019-06-26 | 2023-03-10 | デジタルｐｃｒの測定方法および測定装置 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US20220364164A1 (https=) |
| JP (2) | JP7245734B2 (https=) |
| WO (1) | WO2020261858A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7245734B2 (ja) | 2019-06-26 | 2023-03-24 | 株式会社日立製作所 | デジタルｐｃｒの測定方法および測定装置 |
| WO2022259334A1 (ja) * | 2021-06-07 | 2022-12-15 | 株式会社日立ハイテク | Ｄｎａ検出方法およびｄｎａ検出システム |
| CN113670877B (zh) * | 2021-08-25 | 2022-05-10 | 华中科技大学 | 用于高通量数字pcr检测的斜置上顶式高斯光片成像系统 |
| CN114134210A (zh) * | 2021-12-01 | 2022-03-04 | 艾普拜生物科技(苏州)有限公司 | 数字pcr多靶标基因检测中荧光通道重叠区域信号的再分类分析方法 |
| WO2023218588A1 (ja) * | 2022-05-12 | 2023-11-16 | 株式会社日立ハイテク | 計測デバイスおよび測定方法 |
| JP2025034360A (ja) * | 2023-08-31 | 2025-03-13 | 株式会社日立製作所 | Ｄｎａ検出方法およびｄｎａ検出キット |
| WO2025234041A1 (ja) * | 2024-05-09 | 2025-11-13 | 株式会社日立ハイテク | 対象核酸を検出する方法、および対象核酸検出システム |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8450063B2 (en) * | 2009-01-28 | 2013-05-28 | Fluidigm Corporation | Determination of copy number differences by amplification |
| JP6754301B2 (ja) | 2017-01-05 | 2020-09-09 | 株式会社日立製作所 | ドロップレットデジタルｐｃｒの測定方法および測定装置 |
| JP7066540B2 (ja) * | 2018-06-14 | 2022-05-13 | 株式会社日立製作所 | デジタルｐｃｒの測定方法および測定装置 |
| JP7245734B2 (ja) | 2019-06-26 | 2023-03-24 | 株式会社日立製作所 | デジタルｐｃｒの測定方法および測定装置 |
-
2019
- 2019-06-26 JP JP2019118981A patent/JP7245734B2/ja active Active
-
2020
- 2020-05-27 US US17/620,077 patent/US20220364164A1/en active Pending
- 2020-05-27 WO PCT/JP2020/020998 patent/WO2020261858A1/ja not_active Ceased
-
2023
- 2023-03-10 JP JP2023038011A patent/JP7545511B2/ja active Active
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