JP2020534872A5 - - Google Patents

Download PDF

Info

Publication number
JP2020534872A5
JP2020534872A5 JP2020538759A JP2020538759A JP2020534872A5 JP 2020534872 A5 JP2020534872 A5 JP 2020534872A5 JP 2020538759 A JP2020538759 A JP 2020538759A JP 2020538759 A JP2020538759 A JP 2020538759A JP 2020534872 A5 JP2020534872 A5 JP 2020534872A5
Authority
JP
Japan
Prior art keywords
cell
perturbation
functionalized
beads
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2020538759A
Other languages
English (en)
Japanese (ja)
Other versions
JP7361700B2 (ja
JP2020534872A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2018/052497 external-priority patent/WO2019060857A1/en
Publication of JP2020534872A publication Critical patent/JP2020534872A/ja
Publication of JP2020534872A5 publication Critical patent/JP2020534872A5/ja
Priority to JP2023171925A priority Critical patent/JP2024001119A/ja
Application granted granted Critical
Publication of JP7361700B2 publication Critical patent/JP7361700B2/ja
Priority to JP2026000271A priority patent/JP2026062913A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2020538759A 2017-09-25 2018-09-24 オリゴヌクレオチドにコードされた化学ライブラリ Active JP7361700B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2023171925A JP2024001119A (ja) 2017-09-25 2023-10-03 オリゴヌクレオチドにコードされた化学ライブラリ
JP2026000271A JP2026062913A (ja) 2017-09-25 2026-01-05 オリゴヌクレオチドにコードされた化学ライブラリ

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762562912P 2017-09-25 2017-09-25
US201762562905P 2017-09-25 2017-09-25
US62/562,912 2017-09-25
US62/562,905 2017-09-25
PCT/US2018/052497 WO2019060857A1 (en) 2017-09-25 2018-09-24 CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023171925A Division JP2024001119A (ja) 2017-09-25 2023-10-03 オリゴヌクレオチドにコードされた化学ライブラリ

Publications (3)

Publication Number Publication Date
JP2020534872A JP2020534872A (ja) 2020-12-03
JP2020534872A5 true JP2020534872A5 (https=) 2021-11-04
JP7361700B2 JP7361700B2 (ja) 2023-10-16

Family

ID=65807199

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2020538759A Active JP7361700B2 (ja) 2017-09-25 2018-09-24 オリゴヌクレオチドにコードされた化学ライブラリ
JP2023171925A Pending JP2024001119A (ja) 2017-09-25 2023-10-03 オリゴヌクレオチドにコードされた化学ライブラリ
JP2026000271A Pending JP2026062913A (ja) 2017-09-25 2026-01-05 オリゴヌクレオチドにコードされた化学ライブラリ

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2023171925A Pending JP2024001119A (ja) 2017-09-25 2023-10-03 オリゴヌクレオチドにコードされた化学ライブラリ
JP2026000271A Pending JP2026062913A (ja) 2017-09-25 2026-01-05 オリゴヌクレオチドにコードされた化学ライブラリ

Country Status (8)

Country Link
US (8) US11084037B2 (https=)
EP (1) EP3687687A4 (https=)
JP (3) JP7361700B2 (https=)
KR (2) KR102671480B1 (https=)
CN (2) CN111971124B (https=)
CA (1) CA3076747A1 (https=)
IL (1) IL273511A (https=)
WO (2) WO2019060857A1 (https=)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10258590B2 (en) * 2015-10-14 2019-04-16 Alcresta Therapeutics, Inc. Enteral feeding device and related methods of use
CN109563539A (zh) 2016-06-15 2019-04-02 慕尼黑路德维希马克西米利安斯大学 使用dna纳米技术的单分子检测或定量
WO2018151810A1 (en) 2017-02-17 2018-08-23 The Scripps Research Institute Defining rna-small molecule affinity landscapes enables design of a small molecular inhibitor of an oncogenic non-coding rna
US11084037B2 (en) 2017-09-25 2021-08-10 Plexium, Inc. Oligonucleotide encoded chemical libraries
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
GB201817321D0 (en) * 2018-10-24 2018-12-05 Nanna Therapeutics Ltd Microbeads for tagless encoded chemical library screening
CN110359097B (zh) * 2019-07-08 2022-09-20 上海药明康德新药开发有限公司 DNA编码化合物库构建中通过Suzuki偶联反应得到On-DNA芳烃化合物的方法
CN110331447B (zh) * 2019-07-08 2022-05-27 上海药明康德新药开发有限公司 DNA编码化合物库构建中制备On-DNA芳基炔烃化合物的方法
US20220403374A1 (en) * 2019-09-03 2022-12-22 Flexomics, Llc Optically readable barcodes and systems and methods for characterizing molecular interactions
WO2021072306A1 (en) * 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
AU2020395133A1 (en) * 2019-12-06 2022-05-26 Bio-Rad Laboratories, Inc. Sample processing barcoded bead composition, method, manufacturing, and system
GB201918340D0 (en) * 2019-12-12 2020-01-29 Cambridge Entpr Ltd Spatial barcoding
CN115698282A (zh) 2020-01-13 2023-02-03 福路伦特生物科学公司 单细胞测序
US11040343B1 (en) 2020-01-28 2021-06-22 Plexium, Inc. Caps for assay devices
US11027272B1 (en) 2020-01-28 2021-06-08 Plexium, Inc. Transfer dispensers for assay devices
US12577702B2 (en) 2020-01-28 2026-03-17 Plexium, Inc. Oligonucleotide encoded chemical libraries, related systems, devices, and methods for detecting, analyzing, quantifying, and testing biologics/genetics
US11305288B2 (en) 2020-01-28 2022-04-19 Plexium, Inc. Assay devices for combinatorial libraries
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
WO2021195169A1 (en) 2020-03-24 2021-09-30 Fluent Biosciences Inc. Viral detection using template emulsification
CA3185063A1 (en) * 2020-05-25 2021-12-02 Nissan Chemical Corporation Cleavable dna-encoded library
WO2022008641A1 (en) * 2020-07-08 2022-01-13 Roche Sequencing Solutions, Inc. Split-pool synthesis apparatus and methods of performing split-pool synthesis
EP4182477A4 (en) 2020-07-15 2024-09-11 Fluent Biosciences Inc. MULTI-LEVEL LIGATION OLIGOS
WO2022051369A1 (en) * 2020-09-01 2022-03-10 Replay Holdings, Llc Methods and systems for nucleic acid synthesis
KR20240012371A (ko) * 2021-04-16 2024-01-29 플렉시움 인코포레이티드 분석 디바이스용 캡
CN117440860A (zh) * 2021-04-16 2024-01-23 普莱克斯姆公司 用于组合文库的测定装置
EP4351972A4 (en) 2021-06-07 2025-04-30 Plexium, Inc. Transfer dispenser for test devices with bead size exclusion
CN113403690B (zh) * 2021-06-21 2022-07-19 吉林大学 一种dna编码化合物库药物分子垂钓方法
US20240287505A1 (en) 2021-06-24 2024-08-29 Illumina, Inc. Methods and compositions for combinatorial indexing of bead-based nucleic acids
JP2024525724A (ja) * 2021-07-15 2024-07-12 フルーエント バイオサイエンシーズ インコーポレイテッド Ｄｎａコード化ライブラリーの予め鋳型化された即時パーティション化
CN113549676B (zh) * 2021-08-24 2022-12-27 重庆范德瓦尔斯生物科技有限公司 一种基于磁珠的RNA pull down新方法
US20240393342A1 (en) * 2021-09-27 2024-11-28 The University Of Tokyo Cell labeling molecule and method for analyzing cell
US20250215615A1 (en) * 2021-11-24 2025-07-03 Nissan Chemical Corporation Method for evaluating dna-encoded library
WO2023108090A2 (en) * 2021-12-09 2023-06-15 University Of Florida Research Foundation, Incorporated Platform using dna-encoded small molecule libraries and rna selection to design small molecules that target rna
CN118369438A (zh) 2021-12-10 2024-07-19 伯乐实验室有限公司 用形态可调节功能化颗粒进行样品处理的组合物、方法和系统
WO2023174845A1 (en) * 2022-03-13 2023-09-21 Eleven Therapeutics Ltd System and method for high throughput evaluation of chemical modifications of nucleic acid molecules
WO2025159785A1 (en) * 2024-01-23 2025-07-31 Zafrens Inc. Perturbation beads for use in assays
US12577556B2 (en) 2022-05-09 2026-03-17 Zafrens Inc. Perturbation beads for use in assays
CN115058492B (zh) * 2022-07-07 2025-09-19 东南大学 一种基于均分组合编码微球的空间转录组测序文库构建方法
US12534721B2 (en) 2022-10-06 2026-01-27 Illumina, Inc. Quantitative detection and analysis of moleculesip
WO2024091949A1 (en) * 2022-10-24 2024-05-02 1859, Inc. Methods and systems for encoded effector screening
CN119032204A (zh) * 2022-12-29 2024-11-26 伊路敏纳公司 使用组合分割池策略和化学连接产生条形码化小珠的大文库
EP4683618A1 (en) * 2023-03-20 2026-01-28 Fluid Discovery Core-shell gel particles for separating and analyzing rna and dna from a cell
US20250011843A1 (en) * 2023-07-07 2025-01-09 California Institute Of Technology Molecular detection using barcode probes or barcode fusion probes and hybridization chain reaction
WO2025085861A1 (en) * 2023-10-20 2025-04-24 Spectral Therapeutics, Inc. Composite particles for use in generating spectrally encoded libraries of chemical compounds, and methods of making and using the same

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573905A (en) * 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
CN1154640C (zh) 1993-10-01 2004-06-23 纽约市哥伦比亚大学理事 用标示物编码的多元组合化学库
BR9407947A (pt) * 1993-11-02 1996-11-26 Affymax Tech Nv Processo para sintetizar moléculas diversas em uma pluralidade de substratos e uma coleçao molecular etiquetade para realizaçao reaçoes de copulaçao em contas em paralelo para triar uma coleçao molecular etiquetada para detectar a presença de um ou mais diferentes etiquetas e para determinar a sequência de síntese aparelho para reaçoes de copulaçao paralelas em suportes sólidos bloco de alinhamento ótico para uso com um detector ótico sistemas de distribuiçao para distribuir reagentes dispositiv
AU718357B2 (en) * 1995-06-07 2000-04-13 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5958703A (en) * 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
ATE256142T1 (de) * 1998-05-15 2003-12-15 Isis Innovation Bibliotheken von unterschiedlich markierten oligomeren
DE10050085C1 (de) 2000-10-10 2001-10-31 Jochem Koetting Verschlußelement und Verschlußsystem für Behältnisse und Gefäße
WO2003066907A1 (en) 2002-02-05 2003-08-14 Genome Therapeutics Corporation Seal for microtiter plate and methods of use thereof
SE526038C2 (sv) * 2002-07-08 2005-06-21 Gambro Lundia Ab Polymeraffinitetsmatris, förfarande för framställning därav och anvädning därav
US8597597B2 (en) * 2003-06-26 2013-12-03 Seng Enterprises Ltd. Picoliter well holding device and method of making the same
WO2005021151A1 (en) * 2003-08-27 2005-03-10 President And Fellows Of Harvard College Electronic control of fluidic species
CN101189345A (zh) * 2005-02-01 2008-05-28 Ab先进基因分析公司 珠基测序的试剂、方法和文库
US20070065844A1 (en) 2005-06-08 2007-03-22 Massachusetts Institute Of Technology Solution-based methods for RNA expression profiling
DE102006028101B4 (de) * 2006-06-19 2014-02-13 Siemens Aktiengesellschaft Verfahren zur Analyse von amplifizierten Nukleinsäuren
CN100417730C (zh) * 2006-10-10 2008-09-10 东南大学 基于引物延伸的dna测序方法
EP2222832A4 (en) * 2007-11-15 2012-08-29 Seng Entpr Ltd PICOLITER WELL SUPPORT AND METHOD FOR THE PRODUCTION THEREOF
AU2009234373B2 (en) 2008-04-09 2015-09-24 Cornell University Coferons and methods of making and using them
WO2010091144A1 (en) 2009-02-04 2010-08-12 Wake Forest University Health Sciences Compositions, methods, and kits for identifying candidate molecules from encoded chemical libraries
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
SG174617A1 (en) * 2009-04-02 2011-10-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
WO2010141390A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Nucleotide transient binding for sequencing methods
CN102483424B (zh) * 2009-06-26 2014-11-05 哈佛学院院长等 用于流体操作的方法以及微流体装置
GB2497912B (en) * 2010-10-08 2014-06-04 Harvard College High-throughput single cell barcoding
CN102485979B (zh) * 2010-12-02 2014-02-19 深圳华大基因科技服务有限公司 Ffpe样品核酸文库，其构建方法和ffpe样品分析方法
US20130344473A1 (en) 2011-03-09 2013-12-26 Seng Enterprises Ltd. Arresting objects
SG10201605049QA (en) * 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10436778B2 (en) * 2013-03-15 2019-10-08 Plexbio Co., Ltd. Methods and systems for multiplex assays
DE202013003390U1 (de) 2013-04-11 2014-07-14 Brand Gmbh + Co Kg Pipettiervorrichtung mit einer Mikrodosiereinheit
ES2754177T3 (es) 2013-06-12 2020-04-16 Massachusetts Gen Hospital Métodos para la detección multiplexada de moléculas diana y sus usos
AU2014302277A1 (en) * 2013-06-27 2015-12-24 10X Genomics, Inc. Compositions and methods for sample processing
SG11201600853UA (en) * 2013-08-05 2016-03-30 Twist Bioscience Corp De novo synthesized gene libraries
EP2921556A1 (en) * 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
AU2015250034B2 (en) * 2014-04-21 2019-06-20 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US20170212101A1 (en) * 2014-07-18 2017-07-27 Cdi Laboratories, Inc. Methods and compositions to identify, quantify, and characterize target analytes and binding moieties
CN107873054B (zh) * 2014-09-09 2022-07-12 博德研究所 用于复合单细胞核酸分析的基于微滴的方法和设备
ES2965764T3 (es) 2015-02-24 2024-04-16 Hope City Plataformas de cribado de bibliotecas abordadas espacialmente codificadas químicamente
WO2016160844A2 (en) * 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
RU2724998C2 (ru) * 2015-05-11 2020-06-29 Иллюмина, Инк. Платформа для обнаружения и анализа терапевтических агентов
WO2017048975A1 (en) * 2015-09-17 2017-03-23 The Regents Of The University Of California Droplet-trapping devices for bioassays and diagnostics
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
CA3038535A1 (en) 2016-10-01 2018-04-05 Berkeley Lights, Inc. Dna barcode compositions and methods of in situ identification in a microfluidic device
AU2017357638B2 (en) * 2016-11-08 2023-11-16 Nanna Therapeutics Limited Tagless encoded chemical library
EP3538669A4 (en) 2016-11-10 2020-05-20 The Scripps Research Institute SOLID-PHASE COMPOUND LIBRARY CODING WITH POLYNUCLEOTIDE-BASED BAR CODING
US11084037B2 (en) 2017-09-25 2021-08-10 Plexium, Inc. Oligonucleotide encoded chemical libraries
US11584956B2 (en) * 2018-12-21 2023-02-21 Microsoft Technology Licensing, Llc Selectively controllable cleavable linkers

Similar Documents

Publication Publication Date Title
JP2020534872A5 (https=)
US9850482B2 (en) Heterologous DNA barcoding method
US20240182949A1 (en) Methods of identifying multiple epitopes in cells
US20240263353A1 (en) High-throughput single-cell polyomics
US20180340939A1 (en) Multiplex analysis of single cell constituents
FI3896171T3 (fi) Menetelmä polynukleotidien prosessoimiseksi yksittäisten solujen analysointia varten
CN105705659A (zh) 大规模平行单细胞分析
US11814619B2 (en) Compositions, methods, and systems for single cell barcoding and sequencing
CN105264088A (zh) 提高鉴定细胞中的多个表位的动态范围
JP2002533701A5 (https=)
KR20210084441A (ko) 어레이 상에서 인덱스 및 바코드를 사용하여 리간드를 확인하기 위한 방법 및 조성물
CN115315524A (zh) 单细胞组合索引的细胞计量术测序
JP2022519324A5 (https=)
WO2022256612A1 (en) Compositions, methods, and systems for single cell barcoding and sequencing
Kimura et al. Attachment of oligonucleotide probes to poly carbodiimide‐coated glass for microarray applications
DK2771485T3 (en) PROCEDURE FOR IDENTIFICATION OF APTAMER
US11970785B2 (en) Methods and materials for multiplexed collections of functional ligands
Velegraki Panmicrobial microarrays
Khan et al. Microfluidics for single-cell genomics
Yuan et al. An optically decodable bead array for linking imaging and sequencing with single-cell resolution
Zhao et al. Matrix-seq: an adjustable-resolution spatial transcriptomics via microfluidic matrix-based barcoding
US20250034636A1 (en) Single-cell culture and sequencing with lipid-modified oligos
RU2021133017A (ru) Способ и композиция для анализа клеточных компонентов
JP2024512122A (ja) アダプター二量体検出を用いる核酸ライブラリシーケンシング技術
KR20250132854A (ko) 타겟과 결합된 아이디-마이크로파티클를 이용한 병렬 약물스크리닝 방법