JP2020534872A5 - - Google Patents

Download PDF

Info

Publication number
JP2020534872A5
JP2020534872A5 JP2020538759A JP2020538759A JP2020534872A5 JP 2020534872 A5 JP2020534872 A5 JP 2020534872A5 JP 2020538759 A JP2020538759 A JP 2020538759A JP 2020538759 A JP2020538759 A JP 2020538759A JP 2020534872 A5 JP2020534872 A5 JP 2020534872A5
Authority
JP
Japan
Prior art keywords
cell
perturbation
functionalized
beads
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2020538759A
Other languages
English (en)
Japanese (ja)
Other versions
JP2020534872A (ja
JP7361700B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2018/052497 external-priority patent/WO2019060857A1/en
Publication of JP2020534872A publication Critical patent/JP2020534872A/ja
Publication of JP2020534872A5 publication Critical patent/JP2020534872A5/ja
Priority to JP2023171925A priority Critical patent/JP2024001119A/ja
Application granted granted Critical
Publication of JP7361700B2 publication Critical patent/JP7361700B2/ja
Priority to JP2026000271A priority patent/JP2026062913A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2020538759A 2017-09-25 2018-09-24 オリゴヌクレオチドにコードされた化学ライブラリ Active JP7361700B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2023171925A JP2024001119A (ja) 2017-09-25 2023-10-03 オリゴヌクレオチドにコードされた化学ライブラリ
JP2026000271A JP2026062913A (ja) 2017-09-25 2026-01-05 オリゴヌクレオチドにコードされた化学ライブラリ

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762562912P 2017-09-25 2017-09-25
US201762562905P 2017-09-25 2017-09-25
US62/562,912 2017-09-25
US62/562,905 2017-09-25
PCT/US2018/052497 WO2019060857A1 (en) 2017-09-25 2018-09-24 CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023171925A Division JP2024001119A (ja) 2017-09-25 2023-10-03 オリゴヌクレオチドにコードされた化学ライブラリ

Publications (3)

Publication Number Publication Date
JP2020534872A JP2020534872A (ja) 2020-12-03
JP2020534872A5 true JP2020534872A5 (https=) 2021-11-04
JP7361700B2 JP7361700B2 (ja) 2023-10-16

Family

ID=65807199

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2020538759A Active JP7361700B2 (ja) 2017-09-25 2018-09-24 オリゴヌクレオチドにコードされた化学ライブラリ
JP2023171925A Pending JP2024001119A (ja) 2017-09-25 2023-10-03 オリゴヌクレオチドにコードされた化学ライブラリ
JP2026000271A Pending JP2026062913A (ja) 2017-09-25 2026-01-05 オリゴヌクレオチドにコードされた化学ライブラリ

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2023171925A Pending JP2024001119A (ja) 2017-09-25 2023-10-03 オリゴヌクレオチドにコードされた化学ライブラリ
JP2026000271A Pending JP2026062913A (ja) 2017-09-25 2026-01-05 オリゴヌクレオチドにコードされた化学ライブラリ

Country Status (8)

Country Link
US (8) US20190210018A1 (https=)
EP (1) EP3687687A4 (https=)
JP (3) JP7361700B2 (https=)
KR (2) KR102671480B1 (https=)
CN (2) CN111971124B (https=)
CA (1) CA3076747A1 (https=)
IL (1) IL273511A (https=)
WO (2) WO2019060830A1 (https=)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10258590B2 (en) * 2015-10-14 2019-04-16 Alcresta Therapeutics, Inc. Enteral feeding device and related methods of use
EP3472351B1 (de) 2016-06-15 2020-10-28 Ludwig-Maximilians-Universität München Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
WO2018151810A1 (en) 2017-02-17 2018-08-23 The Scripps Research Institute Defining rna-small molecule affinity landscapes enables design of a small molecular inhibitor of an oncogenic non-coding rna
WO2019060830A1 (en) 2017-09-25 2019-03-28 Plexium, Inc. CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
GB201817321D0 (en) * 2018-10-24 2018-12-05 Nanna Therapeutics Ltd Microbeads for tagless encoded chemical library screening
CN110331447B (zh) * 2019-07-08 2022-05-27 上海药明康德新药开发有限公司 DNA编码化合物库构建中制备On-DNA芳基炔烃化合物的方法
CN110359097B (zh) * 2019-07-08 2022-09-20 上海药明康德新药开发有限公司 DNA编码化合物库构建中通过Suzuki偶联反应得到On-DNA芳烃化合物的方法
US20220403374A1 (en) * 2019-09-03 2022-12-22 Flexomics, Llc Optically readable barcodes and systems and methods for characterizing molecular interactions
AU2020361681A1 (en) * 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
US20210171939A1 (en) * 2019-12-06 2021-06-10 Bio-Rad Laboratories, Inc. Sample processing barcoded bead composition, method, manufacturing, and system
GB201918340D0 (en) * 2019-12-12 2020-01-29 Cambridge Entpr Ltd Spatial barcoding
WO2021146184A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Single cell sequencing
US11305288B2 (en) 2020-01-28 2022-04-19 Plexium, Inc. Assay devices for combinatorial libraries
US11027272B1 (en) 2020-01-28 2021-06-08 Plexium, Inc. Transfer dispensers for assay devices
US12577702B2 (en) * 2020-01-28 2026-03-17 Plexium, Inc. Oligonucleotide encoded chemical libraries, related systems, devices, and methods for detecting, analyzing, quantifying, and testing biologics/genetics
US11040343B1 (en) 2020-01-28 2021-06-22 Plexium, Inc. Caps for assay devices
EP4121016A4 (en) 2020-03-16 2024-09-11 Fluent Biosciences Inc. MULTI-OMICS ANALYSIS IN MONODISPERSED DROPLETS
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
KR20230015347A (ko) * 2020-05-25 2023-01-31 닛산 가가쿠 가부시키가이샤 절단 가능한 dna 코드화 라이브러리
WO2022008641A1 (en) * 2020-07-08 2022-01-13 Roche Sequencing Solutions, Inc. Split-pool synthesis apparatus and methods of performing split-pool synthesis
US12241059B2 (en) 2020-07-15 2025-03-04 Illumina, Inc. Tiered ligation oligos
EP4208565A4 (en) * 2020-09-01 2024-10-09 Replay Holdings, LLC Methods and systems for nucleic acid synthesis
CN117355640A (zh) * 2021-04-16 2024-01-05 普莱克斯姆公司 用于测定装置的盖
IL307630A (en) * 2021-04-16 2023-12-01 Plexium Inc Test devices for combinatorial libraries
CA3220008A1 (en) * 2021-06-07 2022-12-15 Plexium, Inc. Transfer dispensers for assay devices with bead size exclusion
CN113403690B (zh) * 2021-06-21 2022-07-19 吉林大学 一种dna编码化合物库药物分子垂钓方法
CN117580959A (zh) * 2021-06-24 2024-02-20 因美纳有限公司 用于基于小珠的核酸的组合索引的方法和组合物
WO2023287957A1 (en) * 2021-07-15 2023-01-19 Fluent Biosciences, Inc. Pre-templated instant partitioning of dna-encoded libraries
CN113549676B (zh) * 2021-08-24 2022-12-27 重庆范德瓦尔斯生物科技有限公司 一种基于磁珠的RNA pull down新方法
US20240393342A1 (en) * 2021-09-27 2024-11-28 The University Of Tokyo Cell labeling molecule and method for analyzing cell
US20250215615A1 (en) * 2021-11-24 2025-07-03 Nissan Chemical Corporation Method for evaluating dna-encoded library
WO2023108090A2 (en) * 2021-12-09 2023-06-15 University Of Florida Research Foundation, Incorporated Platform using dna-encoded small molecule libraries and rna selection to design small molecules that target rna
WO2023107539A1 (en) 2021-12-10 2023-06-15 Bio-Rad Laboratories, Inc. Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles
US20250197937A1 (en) * 2022-03-13 2025-06-19 Eleven Therapeutics Ltd System and method for high throughput evaluation of chemical modifications of nucleic acid molecules
WO2025159784A1 (en) * 2024-01-23 2025-07-31 Zafrens Inc. Capture beads for use in assays
US12577556B2 (en) 2022-05-09 2026-03-17 Zafrens Inc. Perturbation beads for use in assays
CN115058492B (zh) * 2022-07-07 2025-09-19 东南大学 一种基于均分组合编码微球的空间转录组测序文库构建方法
US12534721B2 (en) 2022-10-06 2026-01-27 Illumina, Inc. Quantitative detection and analysis of moleculesip
WO2024091949A1 (en) * 2022-10-24 2024-05-02 1859, Inc. Methods and systems for encoded effector screening
CN119032204A (zh) * 2022-12-29 2024-11-26 伊路敏纳公司 使用组合分割池策略和化学连接产生条形码化小珠的大文库
WO2024196941A1 (en) * 2023-03-20 2024-09-26 Fluid Discovery Core-shell gel particles for separating and analyzing rna and dna from a cell
US20250011843A1 (en) * 2023-07-07 2025-01-09 California Institute Of Technology Molecular detection using barcode probes or barcode fusion probes and hybridization chain reaction
WO2025085861A1 (en) * 2023-10-20 2025-04-24 Spectral Therapeutics, Inc. Composite particles for use in generating spectrally encoded libraries of chemical compounds, and methods of making and using the same

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573905A (en) * 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
CN1154640C (zh) * 1993-10-01 2004-06-23 纽约市哥伦比亚大学理事 用标示物编码的多元组合化学库
CN1134156A (zh) * 1993-11-02 1996-10-23 阿菲马克斯技术公司 合成和筛选多种分子
CA2222581C (en) * 1995-06-07 2004-05-11 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5958703A (en) * 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
EP1428810A3 (en) * 1998-05-15 2004-07-14 Oxford Gene Technology IP Limited Libraries of oligomers labelled with different tags
DE10050085C1 (de) 2000-10-10 2001-10-31 Jochem Koetting Verschlußelement und Verschlußsystem für Behältnisse und Gefäße
AU2003225552A1 (en) * 2002-02-05 2003-09-02 Genome Therapeutics Corporation Seal for microtiter plate and methods of use thereof
SE526038C2 (sv) * 2002-07-08 2005-06-21 Gambro Lundia Ab Polymeraffinitetsmatris, förfarande för framställning därav och anvädning därav
US8597597B2 (en) * 2003-06-26 2013-12-03 Seng Enterprises Ltd. Picoliter well holding device and method of making the same
CN104069784B (zh) * 2003-08-27 2017-01-11 哈佛大学 流体物种的电子控制
CN101189345A (zh) * 2005-02-01 2008-05-28 Ab先进基因分析公司 珠基测序的试剂、方法和文库
US20070065844A1 (en) * 2005-06-08 2007-03-22 Massachusetts Institute Of Technology Solution-based methods for RNA expression profiling
DE102006028101B4 (de) * 2006-06-19 2014-02-13 Siemens Aktiengesellschaft Verfahren zur Analyse von amplifizierten Nukleinsäuren
CN100417730C (zh) * 2006-10-10 2008-09-10 东南大学 基于引物延伸的dna测序方法
EP2222832A4 (en) * 2007-11-15 2012-08-29 Seng Entpr Ltd PICOLITER WELL SUPPORT AND METHOD FOR THE PRODUCTION THEREOF
US8853185B2 (en) 2008-04-09 2014-10-07 Cornell University Coferons and methods of making and using them
WO2010091144A1 (en) 2009-02-04 2010-08-12 Wake Forest University Health Sciences Compositions, methods, and kits for identifying candidate molecules from encoded chemical libraries
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
KR101829182B1 (ko) * 2009-04-02 2018-03-29 플루이다임 코포레이션 표적 핵산의 바코딩을 위한 멀티 프라이머 증폭 방법
WO2010141391A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Mutant dna polymerases
JP5841937B2 (ja) * 2009-06-26 2016-01-13 プレジデント アンド フェローズ オブ ハーバード カレッジ 流体注入
EP2625320B1 (en) * 2010-10-08 2019-03-27 President and Fellows of Harvard College High-throughput single cell barcoding
CN102485979B (zh) * 2010-12-02 2014-02-19 深圳华大基因科技服务有限公司 Ffpe样品核酸文库,其构建方法和ffpe样品分析方法
US20130344473A1 (en) * 2011-03-09 2013-12-26 Seng Enterprises Ltd. Arresting objects
SG10201605049QA (en) * 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10436778B2 (en) * 2013-03-15 2019-10-08 Plexbio Co., Ltd. Methods and systems for multiplex assays
DE202013003390U1 (de) 2013-04-11 2014-07-14 Brand Gmbh + Co Kg Pipettiervorrichtung mit einer Mikrodosiereinheit
EP3008201B1 (en) 2013-06-12 2019-08-07 The General Hospital Corporation Methods for multiplexed detection of target molecules and uses thereof
BR112015032512A8 (pt) 2013-06-27 2020-01-07 10X Genomics Inc método de implementação de código de barras de materiais de amostra
TWI707038B (zh) * 2013-08-05 2020-10-11 美商扭轉生物科技有限公司 重新合成之基因庫
EP2921556A1 (en) * 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
WO2015164212A1 (en) * 2014-04-21 2015-10-29 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US20170212101A1 (en) * 2014-07-18 2017-07-27 Cdi Laboratories, Inc. Methods and compositions to identify, quantify, and characterize target analytes and binding moieties
CN107873054B (zh) * 2014-09-09 2022-07-12 博德研究所 用于复合单细胞核酸分析的基于微滴的方法和设备
NZ734644A (en) 2015-02-24 2023-02-24 Hope City Chemically encoded spatially addressed library screening platforms
WO2016160844A2 (en) * 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
ES2938072T3 (es) * 2015-05-11 2023-04-04 Illumina Inc Plataforma para el descubrimiento y análisis de agentes terapéuticos
WO2017048975A1 (en) * 2015-09-17 2017-03-23 The Regents Of The University Of California Droplet-trapping devices for bioassays and diagnostics
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
EP3519612B1 (en) 2016-10-01 2022-04-06 Berkeley Lights, Inc. Dna barcode compositions and methods of in situ identification in a microfluidic device
AU2017357638B2 (en) * 2016-11-08 2023-11-16 Nanna Therapeutics Limited Tagless encoded chemical library
US20200190507A1 (en) 2016-11-10 2020-06-18 The Scripps Research Institute Encoded Solid Phase Compound Library with Polynucleotide Based Barcoding
WO2019060830A1 (en) 2017-09-25 2019-03-28 Plexium, Inc. CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES
US11584956B2 (en) * 2018-12-21 2023-02-21 Microsoft Technology Licensing, Llc Selectively controllable cleavable linkers

Similar Documents

Publication Publication Date Title
JP2020534872A5 (https=)
US9850482B2 (en) Heterologous DNA barcoding method
US20240182949A1 (en) Methods of identifying multiple epitopes in cells
US20240263353A1 (en) High-throughput single-cell polyomics
CN105705659B (zh) 大规模平行单细胞分析
US20180340939A1 (en) Multiplex analysis of single cell constituents
FI3896171T3 (fi) Menetelmä polynukleotidien prosessoimiseksi yksittäisten solujen analysointia varten
JP2018509915A5 (https=)
US20220049286A1 (en) Methods of identifying multiple epitopes in cells
US11814619B2 (en) Compositions, methods, and systems for single cell barcoding and sequencing
CN105264088A (zh) 提高鉴定细胞中的多个表位的动态范围
JP2002533701A5 (https=)
KR20210084441A (ko) 어레이 상에서 인덱스 및 바코드를 사용하여 리간드를 확인하기 위한 방법 및 조성물
CN115315524A (zh) 单细胞组合索引的细胞计量术测序
Zenke‐Philippi et al. Transcriptome‐based prediction of hybrid performance with unbalanced data from a maize breeding programme
WO2022256612A1 (en) Compositions, methods, and systems for single cell barcoding and sequencing
WO2003068712A3 (fr) Procede de preparation de biopuces a adn et leurs applications
DK2771485T3 (en) PROCEDURE FOR IDENTIFICATION OF APTAMER
SG11202113372WA (en) Methods and compositions for identifying ligands on arrays using indexes and barcodes
US11970785B2 (en) Methods and materials for multiplexed collections of functional ligands
Khan et al. Microfluidics for single-cell genomics
Yuan et al. An optically decodable bead array for linking imaging and sequencing with single-cell resolution
Zhao et al. Matrix-seq: an adjustable-resolution spatial transcriptomics via microfluidic matrix-based barcoding
RU2021133017A (ru) Способ и композиция для анализа клеточных компонентов
JP2024512122A (ja) アダプター二量体検出を用いる核酸ライブラリシーケンシング技術