JP2020522361A - 軟骨組織修復用コラーゲンの製造および使用方法 - Google Patents
軟骨組織修復用コラーゲンの製造および使用方法 Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
- A61L27/3645—Connective tissue
- A61L27/3654—Cartilage, e.g. meniscus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/362—Skin, e.g. dermal papillae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A—HUMAN NECESSITIES
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- A61L2430/00—Materials or treatment for tissue regeneration
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
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Abstract
Description
9Mとなるように添加する。
週目に肉眼で観察した結果、コラーゲンを注入した実験群は、3週目から欠損部位が修復されることを確認することができたが、対照群は、3週間後にも欠損した部位が正常に修復されないことを観察した。以後も、コラーゲンを注入した実験群は、欠損した部位が修復されて軟骨表面が滑らかであるのに対し、生理食塩水を注入した対照群は、欠損した部位の軟骨表面が滑らかでないように維持されていることを確認することができた。
実験群:A、B、C、D、対照群:E、F、G、H、観察視点:3週目(A、E)、6週目(B、F)、9週目(C、G)、12週目(D、H)
(A)実験群6週目の検体(a−1:H&E染色、a−2:1型コラーゲン染色)
(B)実験群、対照群3週目、12週目の検体(b:H&E染色法、c:サフラニンO)(Safranin−O)染色法、d:トルイジンブルー(Toluidin Blue)染色法、e:1型コラーゲン染色法、f:2型コラーゲン染色法、1:実験群3週目、2:実験群12週目、3:対照群3週目、4:対照群12週目)
ain)、硬直度(stiffness)および生活の質(life)を測定して比較する。数値が低いほど関節の機能が良好である。
Claims (8)
- ブタの皮膚組織を切片化して洗浄した後、酵素を処理してコラーゲンを分離し、塩処理および除菌濾過工程、濃縮工程および充填工程を介して、無菌的に注射容器に充填された高濃度のコラーゲンを得る方法において、
前記ブタの皮膚組織からコラーゲンを分離し、注射容器に充填する過程は、
ブタから分離された皮膚組織をエタノール、水酸化ナトリウムを用いて洗浄した後、切片化する段階、
前記切片化された組織を酵素の含まれている酸溶液と混合した後、攪拌して反応させる段階、
前記組織から分離されたコラーゲンを得るためにNaClを添加する段階、
コラーゲン分子量を分離するためにメンブレンフィルタを通過させ、無菌状態のコラーゲンを得るためにフィルタを通過させる段階、
pHおよび温度を用いてコラーゲンを凝集させた後、遠心分離を行って濃縮させる段階、および
前記濃縮されたコラーゲンを滅菌済みの充填器具を用いて注射容器に充填する段階によって行われることを特徴とする、軟骨組織修復用コラーゲンの製造方法。 - エタノールは70%以上、24時間以内で使用されることを特徴とする、請求項1に記載の軟骨組織修復用コラーゲンの製造方法。
- 水酸化ナトリウムはPH11以上、2時間以内で使用されることを特徴とする、請求項1に記載の軟骨組織修復用コラーゲンの製造方法。
- 切片化された組織を酵素の含まれているPH3以下の酸溶液と混合した後、30℃以下の条件で3〜5日間攪拌して反応することを特徴とする、請求項1に記載の軟骨組織修復要コラーゲンの製造方法。
- フィルタは0.22・壕ネ下の孔隙を有することを特徴とする、請求項1に記載の軟骨組織修復用コラーゲンの製造方法。
- 前記組織から分離されたコラーゲンを得るための最終濃度は0.7〜0.9Mであることを特徴とする、請求項1に記載の軟骨組織修復用コラーゲンの製造方法。
- 請求項1記載の方法で製造された軟骨組織修復用コラーゲンを注射容器に充填して関節に注入する方式で使用可能な形態の軟骨組織修復用コラーゲンの使用方法。
- 請求項1記載の方法で製造されたコラーゲンを注射容器に充填し、注射針を連結して適用部位の外科的切開なしに注射針を適用部位に挿入して軟骨修復用コラーゲンを関節に注入することを特徴とする、軟骨組織修復用コラーゲンの使用方法。
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KR1020170076098A KR101863532B1 (ko) | 2017-06-15 | 2017-06-15 | 연골조직 수복용 콜라겐의 제조 및 사용방법 |
KR10-2017-0076098 | 2017-06-15 | ||
PCT/KR2017/007220 WO2018230765A1 (ko) | 2017-06-15 | 2017-07-06 | 연골조직 수복용 콜라겐의 제조 및 사용방법 |
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US (1) | US20200197568A1 (ja) |
EP (1) | EP3643332A4 (ja) |
JP (1) | JP6899455B2 (ja) |
KR (1) | KR101863532B1 (ja) |
CN (1) | CN110769865A (ja) |
AU (2) | AU2017418178A1 (ja) |
BR (1) | BR112019026753B1 (ja) |
CA (1) | CA3067790C (ja) |
MX (1) | MX2019015087A (ja) |
WO (1) | WO2018230765A1 (ja) |
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CN117915933A (zh) | 2021-09-01 | 2024-04-19 | 上海其胜生物制剂有限公司 | 使用含有软骨细胞或干细胞的可注射可原位聚合胶原组合物进行软骨再生 |
KR20230166079A (ko) * | 2021-09-17 | 2023-12-06 | 아크로 바이오메디컬 컴퍼니 엘티디 | 모낭 형성 또는 혈관신생을 촉진하기 위한 콜라겐 입자의 용도 |
KR102645522B1 (ko) * | 2023-10-18 | 2024-03-07 | 윤미경 | 돈피 콜라겐을 함유하는 반려동물 간식 제조방법 및 이에 의해 제조된 반려동물 간식 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004300077A (ja) * | 2003-03-31 | 2004-10-28 | Nitta Gelatin Inc | コラーゲンタンパク質からのエンドトキシン除去方法 |
JP2008543783A (ja) * | 2005-06-10 | 2008-12-04 | セルジーン・コーポレーション | ヒト胎盤のコラーゲン組成物、これらの調製方法、これらの使用方法及び組成物を含むキット。 |
WO2010125722A1 (ja) * | 2009-05-01 | 2010-11-04 | 国立大学法人 千葉大学 | 人工コラーゲンを用いた軟骨培養用基材及び該基材を用いた軟骨再生治療方法 |
KR20150102865A (ko) * | 2014-02-28 | 2015-09-08 | 전남대학교산학협력단 | 생체적합성 콜라겐 및 이의 제조방법 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5866165A (en) * | 1997-01-15 | 1999-02-02 | Orquest, Inc. | Collagen-polysaccharide matrix for bone and cartilage repair |
AU2002247044B2 (en) | 2001-01-30 | 2006-11-16 | Orthogene, Inc. | Compositions and methods for the treatment and repair of defects or lesions in articular cartilage using synovial-derived tissue or cells |
AUPS242702A0 (en) * | 2002-05-21 | 2002-06-13 | Colltech Australia Limited | Improved method for the extraction and purification of collagen |
KR100676285B1 (ko) * | 2005-03-11 | 2007-01-30 | 세원셀론텍(주) | 동물의 다양한 조직으로부터의 콜라겐 분리방법 및 콜라겐 용액의 제조방법 그리고 이를 이용하여 생산한 매트릭스 |
KR100684932B1 (ko) | 2005-04-13 | 2007-02-20 | (주)필미아젠 | 중간엽 줄기세포와 초음파 자극을 이용하여 연골조직을재생하는 방법 |
KR101114773B1 (ko) | 2009-10-23 | 2012-03-05 | 세원셀론텍(주) | 연골조직 수복용 조성물의 제조방법 |
CN101934092A (zh) * | 2010-08-27 | 2011-01-05 | 中国人民解放军第三军医大学第一附属医院 | 用于软骨再生修复的注射型软骨仿生基质材料及使用方法 |
CN102488925A (zh) * | 2011-12-29 | 2012-06-13 | 成都普川生物医用材料股份有限公司 | 一种可注射的关节软骨组织修复材料及其制备方法 |
KR101279812B1 (ko) | 2012-05-16 | 2013-06-28 | 세원셀론텍(주) | 연골조직 수복용 조성물의 제조방법 |
CN103272278B (zh) * | 2013-05-28 | 2015-04-15 | 北京博辉瑞进生物科技有限公司 | 一种动物源性植入性医用生物材料的制备方法 |
CN103539955B (zh) * | 2013-08-23 | 2017-07-28 | 浙江优必优生物科技有限公司 | 一种人体注射用长效、脱敏、内融型胶原蛋白细胞支架及其制作方法 |
KR101531479B1 (ko) * | 2014-11-21 | 2015-06-26 | 세원셀론텍(주) | 의료용 재료로 사용하기 위한 고농도 콜라겐 제조방법 |
-
2017
- 2017-06-15 KR KR1020170076098A patent/KR101863532B1/ko active IP Right Grant
- 2017-07-06 US US16/622,397 patent/US20200197568A1/en not_active Abandoned
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- 2017-07-06 CN CN201780092049.6A patent/CN110769865A/zh active Pending
- 2017-07-06 MX MX2019015087A patent/MX2019015087A/es unknown
- 2017-07-06 WO PCT/KR2017/007220 patent/WO2018230765A1/ko unknown
- 2017-07-06 AU AU2017418178A patent/AU2017418178A1/en not_active Abandoned
- 2017-07-06 JP JP2019568059A patent/JP6899455B2/ja active Active
- 2017-07-06 CA CA3067790A patent/CA3067790C/en active Active
-
2021
- 2021-12-15 AU AU2021286330A patent/AU2021286330A1/en not_active Abandoned
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004300077A (ja) * | 2003-03-31 | 2004-10-28 | Nitta Gelatin Inc | コラーゲンタンパク質からのエンドトキシン除去方法 |
JP2008543783A (ja) * | 2005-06-10 | 2008-12-04 | セルジーン・コーポレーション | ヒト胎盤のコラーゲン組成物、これらの調製方法、これらの使用方法及び組成物を含むキット。 |
WO2010125722A1 (ja) * | 2009-05-01 | 2010-11-04 | 国立大学法人 千葉大学 | 人工コラーゲンを用いた軟骨培養用基材及び該基材を用いた軟骨再生治療方法 |
KR20150102865A (ko) * | 2014-02-28 | 2015-09-08 | 전남대학교산학협력단 | 생체적합성 콜라겐 및 이의 제조방법 |
Non-Patent Citations (1)
Title |
---|
BMC MUSCULOSKELETAL DISORDERS, vol. Vol.17:94, JPN6020037706, 2016, pages 1 - 8, ISSN: 0004360775 * |
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KR101863532B1 (ko) | 2018-06-01 |
JP6899455B2 (ja) | 2021-07-14 |
CA3067790A1 (en) | 2018-12-20 |
BR112019026753B1 (pt) | 2023-01-24 |
AU2017418178A1 (en) | 2020-01-16 |
CN110769865A (zh) | 2020-02-07 |
EP3643332A1 (en) | 2020-04-29 |
BR112019026753A2 (pt) | 2020-06-30 |
US20200197568A1 (en) | 2020-06-25 |
AU2021286330A1 (en) | 2022-01-20 |
EP3643332A4 (en) | 2021-03-03 |
MX2019015087A (es) | 2020-10-05 |
CA3067790C (en) | 2024-05-28 |
WO2018230765A1 (ko) | 2018-12-20 |
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