JP2020513765A5 - - Google Patents
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- Publication number
- JP2020513765A5 JP2020513765A5 JP2019535360A JP2019535360A JP2020513765A5 JP 2020513765 A5 JP2020513765 A5 JP 2020513765A5 JP 2019535360 A JP2019535360 A JP 2019535360A JP 2019535360 A JP2019535360 A JP 2019535360A JP 2020513765 A5 JP2020513765 A5 JP 2020513765A5
- Authority
- JP
- Japan
- Prior art keywords
- stem cells
- hpsc
- cell
- formulation according
- formulation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 claims 24
- 238000009472 formulation Methods 0.000 claims 23
- 238000000034 method Methods 0.000 claims 16
- 210000000130 stem cell Anatomy 0.000 claims 12
- 210000004027 cell Anatomy 0.000 claims 8
- 238000004113 cell culture Methods 0.000 claims 6
- 238000003306 harvesting Methods 0.000 claims 5
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 claims 4
- 239000002609 medium Substances 0.000 claims 4
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 3
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims 2
- 239000001509 sodium citrate Substances 0.000 claims 2
- 238000004114 suspension culture Methods 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 2
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 230000003833 cell viability Effects 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 claims 1
- 239000002738 chelating agent Substances 0.000 claims 1
- 238000005138 cryopreservation Methods 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000004069 differentiation Effects 0.000 claims 1
- 210000001671 embryonic stem cell Anatomy 0.000 claims 1
- 210000002514 epidermal stem cell Anatomy 0.000 claims 1
- 238000010362 genome editing Methods 0.000 claims 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims 1
- 238000013537 high throughput screening Methods 0.000 claims 1
- 210000001178 neural stem cell Anatomy 0.000 claims 1
- 230000003204 osmotic effect Effects 0.000 claims 1
- 238000007747 plating Methods 0.000 claims 1
- 238000010992 reflux Methods 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 210000001988 somatic stem cell Anatomy 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762447262P | 2017-01-17 | 2017-01-17 | |
| US62/447,262 | 2017-01-17 | ||
| PCT/US2018/014032 WO2018136500A1 (en) | 2017-01-17 | 2018-01-17 | Passaging and harvesting formulation for single-cell human pluripotent stem cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020513765A JP2020513765A (ja) | 2020-05-21 |
| JP2020513765A5 true JP2020513765A5 (enExample) | 2021-02-25 |
| JP7092309B2 JP7092309B2 (ja) | 2022-06-28 |
Family
ID=61163807
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019535360A Active JP7092309B2 (ja) | 2017-01-17 | 2018-01-17 | 単一細胞ヒト多能性幹細胞の継代及び採取用配合物 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20190359946A1 (enExample) |
| EP (1) | EP3555264A1 (enExample) |
| JP (1) | JP7092309B2 (enExample) |
| KR (1) | KR102580997B1 (enExample) |
| CN (1) | CN110291189A (enExample) |
| CA (1) | CA3050259A1 (enExample) |
| IL (1) | IL267628B2 (enExample) |
| WO (1) | WO2018136500A1 (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20220113349A (ko) * | 2019-12-11 | 2022-08-12 | 리페아론 게엠베하 | 생물반응기의 현탁액에서 줄기세포의 확장 |
| CN114807018B (zh) * | 2022-02-28 | 2023-08-01 | 深圳市旷逸生物科技有限公司 | 一种多能干细胞的传代方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3255142A1 (en) * | 2009-10-19 | 2017-12-13 | Cellular Dynamics International, Inc. | Cardiomyocyte production |
| CA2831609C (en) * | 2011-03-30 | 2019-06-11 | Cellular Dynamics International, Inc. | Priming of pluripotent stem cells for neural differentiation |
| EP2718424B1 (en) * | 2011-05-17 | 2018-10-03 | Lonza Walkersville, Inc. | Passaging and harvesting formulation and method for human pluripotent stem cells |
| US9694036B2 (en) * | 2014-03-21 | 2017-07-04 | Cellular Dynamics International, Inc. | Production of midbrain dopaminergic neurons and methods for the use thereof |
-
2018
- 2018-01-17 KR KR1020197023895A patent/KR102580997B1/ko active Active
- 2018-01-17 JP JP2019535360A patent/JP7092309B2/ja active Active
- 2018-01-17 IL IL267628A patent/IL267628B2/en unknown
- 2018-01-17 CN CN201880007148.4A patent/CN110291189A/zh active Pending
- 2018-01-17 WO PCT/US2018/014032 patent/WO2018136500A1/en not_active Ceased
- 2018-01-17 CA CA3050259A patent/CA3050259A1/en active Pending
- 2018-01-17 EP EP18703413.7A patent/EP3555264A1/en active Pending
- 2018-07-17 US US16/478,461 patent/US20190359946A1/en active Pending
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