CA3050259A1 - Passaging and harvesting formulation for single-cell human pluripotent stem cells - Google Patents

Passaging and harvesting formulation for single-cell human pluripotent stem cells Download PDF

Info

Publication number
CA3050259A1
CA3050259A1 CA3050259A CA3050259A CA3050259A1 CA 3050259 A1 CA3050259 A1 CA 3050259A1 CA 3050259 A CA3050259 A CA 3050259A CA 3050259 A CA3050259 A CA 3050259A CA 3050259 A1 CA3050259 A1 CA 3050259A1
Authority
CA
Canada
Prior art keywords
formulation
cell
cells
passaging
hpscs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3050259A
Other languages
English (en)
French (fr)
Inventor
Ying Nie
Jonathan Allen Rowley
Thomas Fellner
Patrick Walsh
Behnam Ahmadian Baghbaderani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza Walkersville Inc
Original Assignee
Lonza Walkersville Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Walkersville Inc filed Critical Lonza Walkersville Inc
Publication of CA3050259A1 publication Critical patent/CA3050259A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3050259A 2017-01-17 2018-01-17 Passaging and harvesting formulation for single-cell human pluripotent stem cells Pending CA3050259A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762447262P 2017-01-17 2017-01-17
US62/447,262 2017-01-17
PCT/US2018/014032 WO2018136500A1 (en) 2017-01-17 2018-01-17 Passaging and harvesting formulation for single-cell human pluripotent stem cells

Publications (1)

Publication Number Publication Date
CA3050259A1 true CA3050259A1 (en) 2018-07-26

Family

ID=61163807

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3050259A Pending CA3050259A1 (en) 2017-01-17 2018-01-17 Passaging and harvesting formulation for single-cell human pluripotent stem cells

Country Status (8)

Country Link
US (1) US20190359946A1 (enExample)
EP (1) EP3555264A1 (enExample)
JP (1) JP7092309B2 (enExample)
KR (1) KR102580997B1 (enExample)
CN (1) CN110291189A (enExample)
CA (1) CA3050259A1 (enExample)
IL (1) IL267628B2 (enExample)
WO (1) WO2018136500A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230059873A1 (en) * 2019-12-11 2023-02-23 Repairon Gmbh Expansion of stem cells in suspension in a bioreactor
CN114807018B (zh) * 2022-02-28 2023-08-01 深圳市旷逸生物科技有限公司 一种多能干细胞的传代方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2777552C (en) * 2009-10-19 2019-05-21 Cellular Dynamics International, Inc. Cardiomyocyte production
US9487752B2 (en) * 2011-03-30 2016-11-08 Cellular Dynamics International, Inc. Priming of pluripotent stem cells for neural differentiation
WO2012158899A1 (en) * 2011-05-17 2012-11-22 Lonza Walkersville Inc. Passaging and harvesting formulation and method for human pluripotent stem cells
ES2939807T3 (es) * 2014-03-21 2023-04-27 Fujifilm Cellular Dynamics Inc Producción de neuronas dopaminérgicas del mesencéfalo y métodos para su utilización

Also Published As

Publication number Publication date
IL267628B2 (en) 2025-06-01
KR20190104407A (ko) 2019-09-09
JP2020513765A (ja) 2020-05-21
IL267628B1 (en) 2025-02-01
US20190359946A1 (en) 2019-11-28
EP3555264A1 (en) 2019-10-23
CN110291189A (zh) 2019-09-27
IL267628A (en) 2019-08-29
WO2018136500A1 (en) 2018-07-26
JP7092309B2 (ja) 2022-06-28
KR102580997B1 (ko) 2023-09-21

Similar Documents

Publication Publication Date Title
Bajpai et al. Efficient propagation of single cells accutase‐dissociated human embryonic stem cells
US8815585B2 (en) Automated method and apparatus for embryonic stem cell culture
US9157061B2 (en) Methods for culture and production of single cell populations of human embryonic stem cells
US10487312B2 (en) Passaging and harvesting formulation and method for human pluripotent stem cells
Cuesta-Gomez et al. Suspension culture improves iPSC expansion and pluripotency phenotype
MX2011005288A (es) Celulas madre pluripotentes en microportadores.
US20190359946A1 (en) Passaging and harvesting formulation for single-cell human pluripotent stem cells
Chen et al. Expansion of human embryonic stem cells on cellulose microcarriers
CN101443445A (zh) 用于增殖人胚泡衍生干细胞的培养系统和方法
Conley et al. Mouse embryonic stem cell derivation, and mouse and human embryonic stem cell culture and differentiation as embryoid bodies
WO2015008275A1 (en) Methods for large scale generation of stem cells
Moreau et al. Differentiation of Human Pluripotent Stem Cells to Megakaryocytes by Transcription Factor-Driven Forward Programming
Chen et al. Generation of skeletal myocytes from embryonic stem cells through nuclear receptor signaling

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20230116

EEER Examination request

Effective date: 20230116

EEER Examination request

Effective date: 20230116