IL267628B2 - Passaging and harvesting formulation for single-cell human pluripotent stem cells - Google Patents

Passaging and harvesting formulation for single-cell human pluripotent stem cells

Info

Publication number
IL267628B2
IL267628B2 IL267628A IL26762819A IL267628B2 IL 267628 B2 IL267628 B2 IL 267628B2 IL 267628 A IL267628 A IL 267628A IL 26762819 A IL26762819 A IL 26762819A IL 267628 B2 IL267628 B2 IL 267628B2
Authority
IL
Israel
Prior art keywords
formulation
cell
cells
hpscs
culture
Prior art date
Application number
IL267628A
Other languages
English (en)
Hebrew (he)
Other versions
IL267628B1 (en
IL267628A (en
Inventor
Ying Nie
Jonathan Allen Rowley
Thomas Fellner
Patrick Walsh
Baghbaderani Behnam Ahmadian
Original Assignee
Lonza Walkersville Inc
Ying Nie
Jonathan Allen Rowley
Thomas Fellner
Patrick Walsh
Baghbaderani Behnam Ahmadian
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Walkersville Inc, Ying Nie, Jonathan Allen Rowley, Thomas Fellner, Patrick Walsh, Baghbaderani Behnam Ahmadian filed Critical Lonza Walkersville Inc
Publication of IL267628A publication Critical patent/IL267628A/en
Publication of IL267628B1 publication Critical patent/IL267628B1/en
Publication of IL267628B2 publication Critical patent/IL267628B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IL267628A 2017-01-17 2018-01-17 Passaging and harvesting formulation for single-cell human pluripotent stem cells IL267628B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762447262P 2017-01-17 2017-01-17
PCT/US2018/014032 WO2018136500A1 (en) 2017-01-17 2018-01-17 Passaging and harvesting formulation for single-cell human pluripotent stem cells

Publications (3)

Publication Number Publication Date
IL267628A IL267628A (en) 2019-08-29
IL267628B1 IL267628B1 (en) 2025-02-01
IL267628B2 true IL267628B2 (en) 2025-06-01

Family

ID=61163807

Family Applications (1)

Application Number Title Priority Date Filing Date
IL267628A IL267628B2 (en) 2017-01-17 2018-01-17 Passaging and harvesting formulation for single-cell human pluripotent stem cells

Country Status (8)

Country Link
US (1) US20190359946A1 (enExample)
EP (1) EP3555264A1 (enExample)
JP (1) JP7092309B2 (enExample)
KR (1) KR102580997B1 (enExample)
CN (1) CN110291189A (enExample)
CA (1) CA3050259A1 (enExample)
IL (1) IL267628B2 (enExample)
WO (1) WO2018136500A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230059873A1 (en) * 2019-12-11 2023-02-23 Repairon Gmbh Expansion of stem cells in suspension in a bioreactor
CN114807018B (zh) * 2022-02-28 2023-08-01 深圳市旷逸生物科技有限公司 一种多能干细胞的传代方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012158899A1 (en) * 2011-05-17 2012-11-22 Lonza Walkersville Inc. Passaging and harvesting formulation and method for human pluripotent stem cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2777552C (en) * 2009-10-19 2019-05-21 Cellular Dynamics International, Inc. Cardiomyocyte production
US9487752B2 (en) * 2011-03-30 2016-11-08 Cellular Dynamics International, Inc. Priming of pluripotent stem cells for neural differentiation
ES2939807T3 (es) * 2014-03-21 2023-04-27 Fujifilm Cellular Dynamics Inc Producción de neuronas dopaminérgicas del mesencéfalo y métodos para su utilización

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012158899A1 (en) * 2011-05-17 2012-11-22 Lonza Walkersville Inc. Passaging and harvesting formulation and method for human pluripotent stem cells

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ALISON THOMSON ET AL., " HUMAN EMBRYONIC STEM CELLS PASSAGED USING ENZYMATIC METHODS RETAIN A NORMAL KARYOTYPE AND EXPRESS CD30", 3 March 2008 (2008-03-03) *
CATHARINA ELLERSTROM ET AL., " FACILITATED EXPANSION OF HUMAN EMBRIONIC STEM CELLS BY SINGLE-CELL ENZYMATIC DISSOCIATION", 1 July 2007 (2007-07-01) *
DE SMEDT A ET AL., " OPTIMISATION OF THE CELL CULTIVATION METHODS IN THE EMBRYONIC STEM CELL TEST RESULTS IN AN INCREASED DIFFERENTIATION POTENTIAL OF THE CELLS INTO STRONG BEATING MYOCARD CELLS", 1 October 2008 (2008-10-01) *
JONAH COHEN ET AL., " SURVIVAL OF PORCINE MESENCHYMAL STEM CELLS OVER THE ALGINATE RECOVERED CELLULAR METHOD", 1 January 2011 (2011-01-01) *
YING NIE ET AL., " SCALABLE PASSAGING OF ADHERENT HUMAN PLURIPOTENT STEM CELLS", 30 January 2014 (2014-01-30) *
YUNLING BAI ET AL., " ROLE OF IRON AND SODIUM CITRATE IN ANIMAL PROTEIN-FREE CHO CELL CULTURE MEDIUM ON CELL GROWTH AND MONOCLONAL ANTIBODY PRODUCTION", 1 January 2011 (2011-01-01) *

Also Published As

Publication number Publication date
KR20190104407A (ko) 2019-09-09
JP2020513765A (ja) 2020-05-21
IL267628B1 (en) 2025-02-01
US20190359946A1 (en) 2019-11-28
EP3555264A1 (en) 2019-10-23
CN110291189A (zh) 2019-09-27
IL267628A (en) 2019-08-29
WO2018136500A1 (en) 2018-07-26
CA3050259A1 (en) 2018-07-26
JP7092309B2 (ja) 2022-06-28
KR102580997B1 (ko) 2023-09-21

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