JP7092309B2 - 単一細胞ヒト多能性幹細胞の継代及び採取用配合物 - Google Patents

単一細胞ヒト多能性幹細胞の継代及び採取用配合物 Download PDF

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JP7092309B2
JP7092309B2 JP2019535360A JP2019535360A JP7092309B2 JP 7092309 B2 JP7092309 B2 JP 7092309B2 JP 2019535360 A JP2019535360 A JP 2019535360A JP 2019535360 A JP2019535360 A JP 2019535360A JP 7092309 B2 JP7092309 B2 JP 7092309B2
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stem cells
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JP2020513765A (ja
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イン ニエ，
ジョナサン， アレン ローリー，
トーマス フェルナー，
パトリック ウォルシュ，
バグバデラニ， ベナム アフマディアン
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ロンザ ウォーカーズヴィル，インコーポレーテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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JP2019535360A 2017-01-17 2018-01-17 単一細胞ヒト多能性幹細胞の継代及び採取用配合物 Active JP7092309B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762447262P 2017-01-17 2017-01-17
US62/447,262 2017-01-17
PCT/US2018/014032 WO2018136500A1 (en) 2017-01-17 2018-01-17 Passaging and harvesting formulation for single-cell human pluripotent stem cells

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JP2020513765A JP2020513765A (ja) 2020-05-21
JP2020513765A5 JP2020513765A5 (enExample) 2021-02-25
JP7092309B2 true JP7092309B2 (ja) 2022-06-28

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US (1) US20190359946A1 (enExample)
EP (1) EP3555264A1 (enExample)
JP (1) JP7092309B2 (enExample)
KR (1) KR102580997B1 (enExample)
CN (1) CN110291189A (enExample)
CA (1) CA3050259A1 (enExample)
IL (1) IL267628B2 (enExample)
WO (1) WO2018136500A1 (enExample)

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US20230059873A1 (en) * 2019-12-11 2023-02-23 Repairon Gmbh Expansion of stem cells in suspension in a bioreactor
CN114807018B (zh) * 2022-02-28 2023-08-01 深圳市旷逸生物科技有限公司 一种多能干细胞的传代方法

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Publication number Priority date Publication date Assignee Title
JP2014513556A (ja) 2011-05-17 2014-06-05 ロンザ ウォカーズビル インコーポレーティッド ヒト多能性幹細胞を採取および継代するための配合物および方法

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CA2777552C (en) * 2009-10-19 2019-05-21 Cellular Dynamics International, Inc. Cardiomyocyte production
US9487752B2 (en) * 2011-03-30 2016-11-08 Cellular Dynamics International, Inc. Priming of pluripotent stem cells for neural differentiation
ES2939807T3 (es) * 2014-03-21 2023-04-27 Fujifilm Cellular Dynamics Inc Producción de neuronas dopaminérgicas del mesencéfalo y métodos para su utilización

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Publication number Priority date Publication date Assignee Title
JP2014513556A (ja) 2011-05-17 2014-06-05 ロンザ ウォカーズビル インコーポレーティッド ヒト多能性幹細胞を採取および継代するための配合物および方法

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IL267628B2 (en) 2025-06-01
KR20190104407A (ko) 2019-09-09
JP2020513765A (ja) 2020-05-21
IL267628B1 (en) 2025-02-01
US20190359946A1 (en) 2019-11-28
EP3555264A1 (en) 2019-10-23
CN110291189A (zh) 2019-09-27
IL267628A (en) 2019-08-29
WO2018136500A1 (en) 2018-07-26
CA3050259A1 (en) 2018-07-26
KR102580997B1 (ko) 2023-09-21

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