JP2020512845A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2020512845A5 JP2020512845A5 JP2020505541A JP2020505541A JP2020512845A5 JP 2020512845 A5 JP2020512845 A5 JP 2020512845A5 JP 2020505541 A JP2020505541 A JP 2020505541A JP 2020505541 A JP2020505541 A JP 2020505541A JP 2020512845 A5 JP2020512845 A5 JP 2020512845A5
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- ato
- universal sequence
- target polynucleotide
- modified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 31
- 108091033319 polynucleotide Proteins 0.000 claims description 24
- 102000040430 polynucleotide Human genes 0.000 claims description 24
- 239000002157 polynucleotide Substances 0.000 claims description 24
- 108091034117 Oligonucleotide Proteins 0.000 claims description 17
- 230000000295 complement effect Effects 0.000 claims description 15
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 10
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 10
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 230000037452 priming Effects 0.000 claims description 4
- 125000006850 spacer group Chemical group 0.000 claims description 4
- 108091028664 Ribonucleotide Proteins 0.000 claims description 3
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical class O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 3
- 239000002336 ribonucleotide Substances 0.000 claims description 3
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 3
- 102000012410 DNA Ligases Human genes 0.000 claims description 2
- 108010061982 DNA Ligases Proteins 0.000 claims description 2
- 125000003277 amino group Chemical group 0.000 claims description 2
- 239000005546 dideoxynucleotide Substances 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 claims 1
- 108060002716 Exonuclease Proteins 0.000 claims 1
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 102000013165 exonuclease Human genes 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 108010068698 spleen exonuclease Proteins 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 108010010677 Phosphodiesterase I Proteins 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- -1 spacers phosphoramidite Chemical class 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1706059.1 | 2017-04-17 | ||
| GBGB1706059.1A GB201706059D0 (en) | 2017-04-17 | 2017-04-17 | Methods, compositions, and kits for preparing library and targeted sequencing |
| GBGB1708384.1A GB201708384D0 (en) | 2017-05-25 | 2017-05-25 | Methods, compositions, and kits for preparing library and targeted sequencing |
| GB1708384.1 | 2017-05-25 | ||
| GB1719114.9 | 2017-11-17 | ||
| GBGB1719114.9A GB201719114D0 (en) | 2017-11-17 | 2017-11-17 | Methods, compositions, and kits for preparing library and targeted sequencing |
| PCT/GB2018/051000 WO2018193233A1 (en) | 2017-04-17 | 2018-04-17 | Methods, compositions, and kits for preparing nucleic acid libraries |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020512845A JP2020512845A (ja) | 2020-04-30 |
| JP2020512845A5 true JP2020512845A5 (https=) | 2021-04-22 |
| JP7079029B2 JP7079029B2 (ja) | 2022-06-01 |
Family
ID=62089770
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020505541A Active JP7079029B2 (ja) | 2017-04-17 | 2018-04-17 | 核酸ライブラリーを調製するための方法、組成物、およびキット |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US11408025B2 (https=) |
| EP (1) | EP3612642B1 (https=) |
| JP (1) | JP7079029B2 (https=) |
| CN (2) | CN110832084B (https=) |
| AU (1) | AU2018254190B2 (https=) |
| CA (1) | CA3060018A1 (https=) |
| ES (1) | ES3035812T3 (https=) |
| WO (1) | WO2018193233A1 (https=) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2018254190B2 (en) | 2017-04-17 | 2024-05-30 | Guoliang Fu | Methods, compositions, and kits for preparing nucleic acid libraries |
| CA3120713A1 (en) * | 2018-11-21 | 2020-05-28 | Avida Biomed, Inc. | Methods for targeted nucleic acid library formation |
| US11390915B2 (en) * | 2019-04-26 | 2022-07-19 | New England Biolabs, Inc. | Polynucleotide adapter design for reduced bias |
| CN114026249B (zh) | 2019-06-20 | 2024-01-23 | 深圳华大生命科学研究院 | 基于rna样本构建文库的方法及应用 |
| US20220356467A1 (en) * | 2019-06-25 | 2022-11-10 | Board Of Regents, The University Of Texas System | Methods for duplex sequencing of cell-free dna and applications thereof |
| CN121344163A (zh) * | 2020-01-16 | 2026-01-16 | Dnae诊断有限公司 | 用于分离靶多核苷酸的组合物、试剂盒和方法 |
| WO2021232023A2 (en) * | 2020-05-15 | 2021-11-18 | Swift Biosciences, Inc. | Methods for ligation-coupled-pcr |
| MX2023001400A (es) * | 2020-09-11 | 2023-04-25 | Illumina Cambridge Ltd | Metodos para enriquecer una secuencia diana a partir de una genoteca de secuenciación usando adaptadores de horquilla. |
| GB202108427D0 (en) | 2021-06-14 | 2021-07-28 | Fu Guoliang | Methods, compositions, and kits for preparing sequencing library |
| CN113684249B (zh) * | 2021-08-25 | 2024-10-22 | 广州达安基因股份有限公司 | 一种rna和dna二代测序文库的构建方法及二代测序盒 |
| US20250354297A1 (en) * | 2021-10-29 | 2025-11-20 | Board Of Regents, The University Of Texas System | Shielded small nucleotides for intracellular barcoding |
| AU2023221441B2 (en) * | 2022-02-18 | 2026-04-09 | Agilent Technologies, Inc. | Systems and methods for targeted nucleic acid capture and barcoding |
| EP4490313A1 (en) * | 2022-03-09 | 2025-01-15 | New England Biolabs, Inc. | Target enrichment |
| CN117487884B (zh) * | 2022-08-01 | 2025-04-15 | 南京诺唯赞生物科技股份有限公司 | 一种高样品利用率的基于转座酶的测序文库构建方法及相关寡核苷酸 |
| CN120603940A (zh) * | 2022-11-11 | 2025-09-05 | 生米公司 | 用于处理和扩增核酸的方法和组合物 |
| WO2024137316A1 (en) * | 2022-12-23 | 2024-06-27 | Foundation Medicine, Inc. | Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs |
| GB202308392D0 (en) | 2023-06-06 | 2023-07-19 | Fu Guoliang | Methods, compositions, and kits for preparing library and targeted sequencing |
| GB202319353D0 (en) | 2023-12-17 | 2024-01-31 | Fu Guoliang | Tail sequence guided addition of adaptor |
| CN117701679B (zh) * | 2024-02-06 | 2024-06-14 | 中国医学科学院基础医学研究所 | 一种基于5’连接的单链dna特异的高通量测序方法 |
| WO2025184067A1 (en) * | 2024-02-26 | 2025-09-04 | Biomeme, Inc. | Methods and compositions for processing and amplification of nucleic acids |
| WO2026050948A1 (zh) * | 2024-09-04 | 2026-03-12 | 深圳华大生命科学研究院 | 核酸文库的构建方法及应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2501983A1 (en) * | 2002-10-30 | 2004-05-27 | Pamgene B.V. | Improved methods for generating multiple rna copies |
| US20050153333A1 (en) * | 2003-12-02 | 2005-07-14 | Sooknanan Roy R. | Selective terminal tagging of nucleic acids |
| US20120156729A1 (en) * | 2003-12-02 | 2012-06-21 | Sooknanan Roy R | Selective terminal tagging of nucleic acids |
| ATE551349T1 (de) * | 2005-11-30 | 2012-04-15 | Epict Technologies Corp | Methode unter verwendung von reversibel blockierten markierungsoligonukleotiden |
| US7754429B2 (en) * | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
| EP2280081A1 (en) * | 2009-07-31 | 2011-02-02 | Qiagen GmbH | Method of normalized quantification of RNA |
| KR101599555B1 (ko) | 2009-12-24 | 2016-03-03 | 두산인프라코어 주식회사 | 하이브리드용 전력 변환 장치 |
| CA2811185C (en) | 2010-09-21 | 2020-09-22 | Population Genetics Technologies Ltd. | Increasing confidence of allele calls with molecular counting |
| US10017807B2 (en) * | 2013-03-15 | 2018-07-10 | Verinata Health, Inc. | Generating cell-free DNA libraries directly from blood |
| US10053725B2 (en) * | 2013-04-23 | 2018-08-21 | President And Fellows Of Harvard College | In situ interaction determination |
| CA2930942A1 (en) * | 2013-11-18 | 2015-05-21 | Rubicon Genomics, Inc. | Degradable adaptors for background reduction |
| CN104946737B (zh) * | 2013-12-11 | 2019-02-22 | 安可济控股有限公司 | 用于检测罕见序列变体的组合物和方法 |
| CN105793438B (zh) * | 2013-12-15 | 2020-02-11 | 中央研究院 | 未知序列的双股线性核酸的全长扩增方法 |
| ES2819277T3 (es) * | 2014-02-11 | 2021-04-15 | Hoffmann La Roche | Secuenciación dirigida y filtrado de UID |
| EP3169799B1 (en) * | 2014-07-15 | 2019-09-04 | Qiagen Sciences, LLC | Semi-random barcodes for nucleic acid analysis |
| AU2018254190B2 (en) | 2017-04-17 | 2024-05-30 | Guoliang Fu | Methods, compositions, and kits for preparing nucleic acid libraries |
-
2018
- 2018-04-17 AU AU2018254190A patent/AU2018254190B2/en active Active
- 2018-04-17 EP EP18721095.0A patent/EP3612642B1/en active Active
- 2018-04-17 US US16/605,520 patent/US11408025B2/en active Active
- 2018-04-17 JP JP2020505541A patent/JP7079029B2/ja active Active
- 2018-04-17 CN CN201880040163.9A patent/CN110832084B/zh active Active
- 2018-04-17 CA CA3060018A patent/CA3060018A1/en active Pending
- 2018-04-17 ES ES18721095T patent/ES3035812T3/es active Active
- 2018-04-17 CN CN202510735693.7A patent/CN120796437A/zh active Pending
- 2018-04-17 WO PCT/GB2018/051000 patent/WO2018193233A1/en not_active Ceased
-
2022
- 2022-07-20 US US17/869,595 patent/US11993805B2/en active Active
-
2023
- 2023-06-26 US US18/341,446 patent/US12297484B2/en active Active
-
2025
- 2025-05-12 US US19/204,940 patent/US20250270621A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2020512845A5 (https=) | ||
| US11964997B2 (en) | Methods of library construction for polynucleotide sequencing | |
| EP1910549B1 (en) | Analyzing messenger rna and micro rna in the same reaction mixture | |
| JP2017504360A5 (https=) | ||
| CN111868257B (zh) | 用于单分子测序的双链dna模板的生成 | |
| JP6219944B2 (ja) | ５’保護に依存した増幅 | |
| JP2018516569A (ja) | 次世代ゲノムウォーキングのための方法ならびに関連する組成物およびキット | |
| KR20100074188A (ko) | 축퇴 올리고뉴클레오티드 및 이의 용도 | |
| JPH06505872A (ja) | １本鎖直鎖状ｄｎａ鋳型から全長２本鎖ｄｎａを合成する方法 | |
| JP2016511007A (ja) | 鎖になったｒｎａまたはｄｎａのライブラリを生成するための方法、組成物およびキット | |
| BRPI0609031A2 (pt) | métodos para a sìntese de uma molécula de ácido nucleico, para seletivamente amplificar uma seqüência de ácido nucleico alvo de um dna ou de uma mistura de ácidos nucleicos, para amplificar duas ou mais seqüências de nucleotìdeos alvo simultaneamente, para seqüenciar uma molécula de ácido nucleico alvo, para detectar uma molécula de ácido nucleico com diversidade genética, para detectar uma seqüência de nucleotìdeos alvo em uma amostra da ácido nucléico, e para permitir que uma especifidade anelar de um oligonucleotìdeo seja dualmente determinada por meio de uma estrutura do oligonucleotìdeo, e, oligonucleotìdeo de especifidade dual | |
| JP6310099B2 (ja) | プライマーダイマーの増幅を低減する方法 | |
| EP3485030B1 (en) | Method for generating single-stranded circular dna libraries for single molecule sequencing | |
| JP2023002557A (ja) | シングルプライマーからデュアルプライマーのアンプリコンへのスイッチング | |
| JP2019534025A (ja) | 治療用オリゴヌクレオチドの捕捉および検出 | |
| TW200532025A (en) | Method of amplifying nucleic acid | |
| JP2002521070A5 (https=) | ||
| EP4010493B1 (en) | Methods for the multiplexed isothermal amplification of nucleic acid sequences | |
| JP2003504018A5 (https=) | ||
| KR20160098097A (ko) | 마이크로알앤에이 검출용 키트 및 방법 | |
| WO2018232038A1 (en) | Methods of library construction for target polynucleotides | |
| EP3682027B1 (en) | Hybridization-extension-ligation strategy for generating circular single-stranded dna libraries | |
| US9611505B2 (en) | Application of oligo-DT molecules to avoid generation of high molecular PCR products induced by poly-A carrier | |
| EP3277833B1 (en) | Methods to amplify highly uniform and less error prone nucleic acid libraries | |
| JPWO2021146534A5 (https=) |