JP7079029B2 - 核酸ライブラリーを調製するための方法、組成物、およびキット - Google Patents

核酸ライブラリーを調製するための方法、組成物、およびキット Download PDF

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JP7079029B2
JP7079029B2 JP2020505541A JP2020505541A JP7079029B2 JP 7079029 B2 JP7079029 B2 JP 7079029B2 JP 2020505541 A JP2020505541 A JP 2020505541A JP 2020505541 A JP2020505541 A JP 2020505541A JP 7079029 B2 JP7079029 B2 JP 7079029B2
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sequence
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target polynucleotide
primer
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フゥ グオリアン
ダンウェル トーマス
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Crystallography & Structural Chemistry (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2020505541A 2017-04-17 2018-04-17 核酸ライブラリーを調製するための方法、組成物、およびキット Active JP7079029B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GB1706059.1 2017-04-17
GBGB1706059.1A GB201706059D0 (en) 2017-04-17 2017-04-17 Methods, compositions, and kits for preparing library and targeted sequencing
GBGB1708384.1A GB201708384D0 (en) 2017-05-25 2017-05-25 Methods, compositions, and kits for preparing library and targeted sequencing
GB1708384.1 2017-05-25
GB1719114.9 2017-11-17
GBGB1719114.9A GB201719114D0 (en) 2017-11-17 2017-11-17 Methods, compositions, and kits for preparing library and targeted sequencing
PCT/GB2018/051000 WO2018193233A1 (en) 2017-04-17 2018-04-17 Methods, compositions, and kits for preparing nucleic acid libraries

Publications (3)

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JP2020512845A JP2020512845A (ja) 2020-04-30
JP2020512845A5 JP2020512845A5 (https=) 2021-04-22
JP7079029B2 true JP7079029B2 (ja) 2022-06-01

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JP2020505541A Active JP7079029B2 (ja) 2017-04-17 2018-04-17 核酸ライブラリーを調製するための方法、組成物、およびキット

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US (4) US11408025B2 (https=)
EP (1) EP3612642B1 (https=)
JP (1) JP7079029B2 (https=)
CN (2) CN110832084B (https=)
AU (1) AU2018254190B2 (https=)
CA (1) CA3060018A1 (https=)
ES (1) ES3035812T3 (https=)
WO (1) WO2018193233A1 (https=)

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AU2018254190B2 (en) 2017-04-17 2024-05-30 Guoliang Fu Methods, compositions, and kits for preparing nucleic acid libraries
CA3120713A1 (en) * 2018-11-21 2020-05-28 Avida Biomed, Inc. Methods for targeted nucleic acid library formation
US11390915B2 (en) * 2019-04-26 2022-07-19 New England Biolabs, Inc. Polynucleotide adapter design for reduced bias
CN114026249B (zh) 2019-06-20 2024-01-23 深圳华大生命科学研究院 基于rna样本构建文库的方法及应用
US20220356467A1 (en) * 2019-06-25 2022-11-10 Board Of Regents, The University Of Texas System Methods for duplex sequencing of cell-free dna and applications thereof
CN121344163A (zh) * 2020-01-16 2026-01-16 Dnae诊断有限公司 用于分离靶多核苷酸的组合物、试剂盒和方法
WO2021232023A2 (en) * 2020-05-15 2021-11-18 Swift Biosciences, Inc. Methods for ligation-coupled-pcr
MX2023001400A (es) * 2020-09-11 2023-04-25 Illumina Cambridge Ltd Metodos para enriquecer una secuencia diana a partir de una genoteca de secuenciación usando adaptadores de horquilla.
GB202108427D0 (en) 2021-06-14 2021-07-28 Fu Guoliang Methods, compositions, and kits for preparing sequencing library
CN113684249B (zh) * 2021-08-25 2024-10-22 广州达安基因股份有限公司 一种rna和dna二代测序文库的构建方法及二代测序盒
US20250354297A1 (en) * 2021-10-29 2025-11-20 Board Of Regents, The University Of Texas System Shielded small nucleotides for intracellular barcoding
AU2023221441B2 (en) * 2022-02-18 2026-04-09 Agilent Technologies, Inc. Systems and methods for targeted nucleic acid capture and barcoding
EP4490313A1 (en) * 2022-03-09 2025-01-15 New England Biolabs, Inc. Target enrichment
CN117487884B (zh) * 2022-08-01 2025-04-15 南京诺唯赞生物科技股份有限公司 一种高样品利用率的基于转座酶的测序文库构建方法及相关寡核苷酸
CN120603940A (zh) * 2022-11-11 2025-09-05 生米公司 用于处理和扩增核酸的方法和组合物
WO2024137316A1 (en) * 2022-12-23 2024-06-27 Foundation Medicine, Inc. Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs
GB202308392D0 (en) 2023-06-06 2023-07-19 Fu Guoliang Methods, compositions, and kits for preparing library and targeted sequencing
GB202319353D0 (en) 2023-12-17 2024-01-31 Fu Guoliang Tail sequence guided addition of adaptor
CN117701679B (zh) * 2024-02-06 2024-06-14 中国医学科学院基础医学研究所 一种基于5’连接的单链dna特异的高通量测序方法
WO2025184067A1 (en) * 2024-02-26 2025-09-04 Biomeme, Inc. Methods and compositions for processing and amplification of nucleic acids
WO2026050948A1 (zh) * 2024-09-04 2026-03-12 深圳华大生命科学研究院 核酸文库的构建方法及应用

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US20050153333A1 (en) 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
JP2006504440A (ja) 2002-10-30 2006-02-09 パムジーン ベー．ベー． 複数のｒｎａコピーを作成するための改良方法
WO2007062495A1 (en) 2005-11-30 2007-06-07 Roy Rabindranauth Sooknanan Selective terminal tagging of nucleic acids
JP2013500706A (ja) 2009-07-31 2013-01-10 キアゲン ゲーエムベーハー Ｒｎａの正規化した定量化方法

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JP2006504440A (ja) 2002-10-30 2006-02-09 パムジーン ベー．ベー． 複数のｒｎａコピーを作成するための改良方法
US20050153333A1 (en) 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
WO2007062495A1 (en) 2005-11-30 2007-06-07 Roy Rabindranauth Sooknanan Selective terminal tagging of nucleic acids
JP2013500706A (ja) 2009-07-31 2013-01-10 キアゲン ゲーエムベーハー Ｒｎａの正規化した定量化方法

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Publication number Publication date
US11993805B2 (en) 2024-05-28
AU2018254190A1 (en) 2019-10-31
US20230121409A1 (en) 2023-04-20
CN120796437A (zh) 2025-10-17
WO2018193233A1 (en) 2018-10-25
EP3612642A1 (en) 2020-02-26
US12297484B2 (en) 2025-05-13
CA3060018A1 (en) 2018-10-25
AU2018254190B2 (en) 2024-05-30
EP3612642C0 (en) 2025-07-09
EP3612642B1 (en) 2025-07-09
US20250270621A1 (en) 2025-08-28
US11408025B2 (en) 2022-08-09
ES3035812T3 (en) 2025-09-09
US20200115736A1 (en) 2020-04-16
JP2020512845A (ja) 2020-04-30
CN110832084B (zh) 2025-06-20
US20240002906A1 (en) 2024-01-04
CN110832084A (zh) 2020-02-21

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