JP2020506687A5 - - Google Patents
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- Publication number
- JP2020506687A5 JP2020506687A5 JP2019539789A JP2019539789A JP2020506687A5 JP 2020506687 A5 JP2020506687 A5 JP 2020506687A5 JP 2019539789 A JP2019539789 A JP 2019539789A JP 2019539789 A JP2019539789 A JP 2019539789A JP 2020506687 A5 JP2020506687 A5 JP 2020506687A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleotide
- strand
- support
- chain
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002773 nucleotide Substances 0.000 claims description 308
- 125000003729 nucleotide group Chemical group 0.000 claims description 308
- 108091033319 polynucleotide Proteins 0.000 claims description 150
- 102000040430 polynucleotide Human genes 0.000 claims description 150
- 239000002157 polynucleotide Substances 0.000 claims description 150
- 238000000034 method Methods 0.000 claims description 111
- 238000003776 cleavage reaction Methods 0.000 claims description 37
- 230000007017 scission Effects 0.000 claims description 37
- 230000015572 biosynthetic process Effects 0.000 claims description 25
- 230000002441 reversible effect Effects 0.000 claims description 25
- 238000003786 synthesis reaction Methods 0.000 claims description 25
- 102000004190 Enzymes Human genes 0.000 claims description 14
- 108090000790 Enzymes Proteins 0.000 claims description 14
- 230000000295 complement effect Effects 0.000 claims description 13
- 125000000524 functional group Chemical group 0.000 claims description 8
- 230000005783 single-strand break Effects 0.000 claims description 6
- -1 bromoacetyl group Chemical group 0.000 claims description 5
- 229920002401 polyacrylamide Polymers 0.000 claims description 5
- 102000004099 Deoxyribonuclease (Pyrimidine Dimer) Human genes 0.000 claims description 4
- 108010082610 Deoxyribonuclease (Pyrimidine Dimer) Proteins 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims description 4
- 102000004317 Lyases Human genes 0.000 claims description 3
- 108090000856 Lyases Proteins 0.000 claims description 3
- 230000002194 synthesizing effect Effects 0.000 claims description 3
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical group OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 claims description 2
- 125000003277 amino group Chemical group 0.000 claims description 2
- 230000001268 conjugating effect Effects 0.000 claims description 2
- 238000000338 in vitro Methods 0.000 claims description 2
- 238000010348 incorporation Methods 0.000 claims description 2
- 230000010354 integration Effects 0.000 claims description 2
- 125000001391 thioamide group Chemical group 0.000 claims description 2
- 125000003396 thiol group Chemical group [H]S* 0.000 claims description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims 1
- 239000002131 composite material Substances 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 description 12
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical group [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000002493 microarray Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- SGPUHRSBWMQRAN-UHFFFAOYSA-N 2-[bis(1-carboxyethyl)phosphanyl]propanoic acid Chemical compound OC(=O)C(C)P(C(C)C(O)=O)C(C)C(O)=O SGPUHRSBWMQRAN-UHFFFAOYSA-N 0.000 description 2
- 108010034927 3-methyladenine-DNA glycosylase Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 102000012410 DNA Ligases Human genes 0.000 description 2
- 108010061982 DNA Ligases Proteins 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 102100039128 DNA-3-methyladenine glycosylase Human genes 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 241000205188 Thermococcus Species 0.000 description 1
- 108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- KVKFRMCSXWQSNT-UHFFFAOYSA-N n,n'-dimethylethane-1,2-diamine Chemical group CNCCNC KVKFRMCSXWQSNT-UHFFFAOYSA-N 0.000 description 1
- KVLNTIPUCYZQHA-UHFFFAOYSA-N n-[5-[(2-bromoacetyl)amino]pentyl]prop-2-enamide Chemical compound BrCC(=O)NCCCCCNC(=O)C=C KVLNTIPUCYZQHA-UHFFFAOYSA-N 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1700937.4A GB2559117B (en) | 2017-01-19 | 2017-01-19 | Double stranded polynucleotide synthesis method, kit and system |
| GB1700937.4 | 2017-01-19 | ||
| PCT/GB2018/050165 WO2018134616A1 (en) | 2017-01-19 | 2018-01-19 | Methods and reagents for synthesising polynucleotide molecules |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020506687A JP2020506687A (ja) | 2020-03-05 |
| JP2020506687A5 true JP2020506687A5 (https=) | 2021-03-04 |
| JP7295021B2 JP7295021B2 (ja) | 2023-06-20 |
Family
ID=58463198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019539789A Active JP7295021B2 (ja) | 2017-01-19 | 2018-01-19 | ポリヌクレオチド分子を合成するための方法および試薬 |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US10927394B2 (https=) |
| EP (2) | EP3950115A1 (https=) |
| JP (1) | JP7295021B2 (https=) |
| KR (1) | KR102618717B1 (https=) |
| CN (1) | CN110461463B (https=) |
| AU (1) | AU2018209365B2 (https=) |
| CA (1) | CA3050822C (https=) |
| GB (1) | GB2559117B (https=) |
| SG (1) | SG11201906553TA (https=) |
| WO (1) | WO2018134616A1 (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2559117B (en) | 2017-01-19 | 2019-11-27 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method, kit and system |
| GB2574197B (en) * | 2018-05-23 | 2022-01-05 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method and system. |
| GB201811811D0 (en) * | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
| GB201811813D0 (en) * | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
| GB201811810D0 (en) * | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
| EP3826762A1 (en) | 2018-07-23 | 2021-06-02 | DNA Script | Massively parallel enzymatic synthesis of nucleic acid strands |
| WO2020165137A1 (en) | 2019-02-12 | 2020-08-20 | Dna Script | Efficient product cleavage in template-free enzymatic synthesis of polynucleotides. |
| GB201913039D0 (en) | 2019-09-10 | 2019-10-23 | Oxford Nanopore Tech Ltd | Polynicleotide synthesis method kit and system |
| GB201914282D0 (en) * | 2019-10-03 | 2019-11-20 | Stemnovate Ltd | Method |
| US20210317424A1 (en) * | 2019-12-23 | 2021-10-14 | Cheng-Yao Chen | Method and kit for template-independent nucleic acid synthesis |
| US20230117083A1 (en) * | 2020-01-22 | 2023-04-20 | Nuclera Nucleics Ltd. | Methods of nucleic acid synthesis |
| GB202013102D0 (en) * | 2020-08-21 | 2020-10-07 | Nuclera Nucleics Ltd | Methods of nucleic acid synthesis |
| GB202002077D0 (en) * | 2020-02-14 | 2020-04-01 | Nuclera Nucleics Ltd | Methods for cell-free protein expression |
| US20230235373A1 (en) * | 2020-06-12 | 2023-07-27 | Synhelix | Controlled template-independent synthesis of nucleic acids using thermostable enzymes |
| CN112159836B (zh) * | 2020-10-09 | 2022-09-06 | 中国科学院长春应用化学研究所 | 一种双发夹连接介导恒温扩增的核酸检测新方法 |
| EP4363608B1 (en) * | 2021-07-01 | 2025-04-02 | Miltenyi Biotec B.V. & Co. KG | Unit-dna composition for spatial barcoding and sequencing |
| WO2023196499A1 (en) * | 2022-04-07 | 2023-10-12 | Twist Bioscience Corporation | Substrate cleavage for nucleic acid synthesis |
| GB202309649D0 (en) * | 2023-06-27 | 2023-08-09 | Oxford Nanopore Tech Plc | Method |
Family Cites Families (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5438131A (en) * | 1992-09-16 | 1995-08-01 | Bergstrom; Donald E. | 3-nitropyrrole nucleoside |
| DE19925862A1 (de) | 1999-06-07 | 2000-12-14 | Diavir Gmbh | Verfahren zur Synthese von DNA-Fragmenten |
| US8137906B2 (en) | 1999-06-07 | 2012-03-20 | Sloning Biotechnology Gmbh | Method for the synthesis of DNA fragments |
| US6479262B1 (en) * | 2000-05-16 | 2002-11-12 | Hercules, Incorporated | Solid phase enzymatic assembly of polynucleotides |
| EP1314783B1 (de) | 2001-11-22 | 2008-11-19 | Sloning BioTechnology GmbH | Nukleinsäure-Linker und deren Verwendung in der Gensynthese |
| DE10223057A1 (de) | 2002-05-24 | 2003-12-11 | Basf Ag | Verfahren zur Erzeugung von Polynukleotidmolekülen |
| WO2007083766A1 (ja) * | 2006-01-20 | 2007-07-26 | Olympus Corporation | 分子内プローブによる核酸配列の検出方法 |
| NZ590265A (en) * | 2008-06-11 | 2012-11-30 | Lasergen Inc | Nucleotides and nucleosides and methods for their use in dna sequencing |
| WO2010025310A2 (en) * | 2008-08-27 | 2010-03-04 | Westend Asset Clearinghouse Company, Llc | Methods and devices for high fidelity polynucleotide synthesis |
| KR101107315B1 (ko) * | 2009-09-29 | 2012-01-20 | 한국과학기술연구원 | ３′―하이드록실기에 형광을 띄는 장애그룹이 부착된 뉴클레오시드 삼인산을 가역적 종결자로서 이용한 ｄｎａ 염기서열 분석 방법 |
| US9187777B2 (en) * | 2010-05-28 | 2015-11-17 | Gen9, Inc. | Methods and devices for in situ nucleic acid synthesis |
| US8653832B2 (en) | 2010-07-06 | 2014-02-18 | Sharp Kabushiki Kaisha | Array element circuit and active matrix device |
| CN103502448B (zh) * | 2010-11-12 | 2017-03-29 | Gen9股份有限公司 | 核酸合成的方法和设备 |
| US8828336B2 (en) | 2011-02-02 | 2014-09-09 | Sharp Kabushiki Kaisha | Active matrix device |
| US10378051B2 (en) * | 2011-09-29 | 2019-08-13 | Illumina Cambridge Limited | Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing |
| CA2877094A1 (en) * | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| CN107760772B (zh) * | 2012-08-24 | 2022-02-01 | 生命技术公司 | 用于核酸配对末端测序的方法、组合物、系统、仪器和试剂盒 |
| JP6375301B2 (ja) | 2012-10-26 | 2018-08-15 | オックスフォード ナノポール テクノロジーズ リミテッド | 液滴界面 |
| US9492824B2 (en) | 2013-01-16 | 2016-11-15 | Sharp Kabushiki Kaisha | Efficient dilution method, including washing method for immunoassay |
| US9169573B2 (en) | 2013-01-23 | 2015-10-27 | Sharp Kabushiki Kaisha | AM-EWOD device and method of driving with variable voltage AC driving |
| US20140274729A1 (en) * | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Methods, compositions and kits for generation of stranded rna or dna libraries |
| US9279149B2 (en) | 2013-04-02 | 2016-03-08 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
| US9771613B2 (en) | 2013-04-02 | 2017-09-26 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acid |
| US8808989B1 (en) | 2013-04-02 | 2014-08-19 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
| US10683536B2 (en) * | 2013-04-02 | 2020-06-16 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| JP6448097B2 (ja) * | 2013-10-15 | 2019-01-09 | モレキュラー アセンブリーズ， インコーポレイテッド | 核酸を合成するための方法および装置 |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| CN111534504B (zh) | 2014-01-22 | 2024-06-21 | 牛津纳米孔科技公开有限公司 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
| FR3020071B1 (fr) | 2014-04-17 | 2017-12-22 | Dna Script | Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede |
| US20170218416A1 (en) | 2014-05-16 | 2017-08-03 | The Regents Of The University Of California | Compositions and methods for single-molecule construction of dna |
| FR3025201B1 (fr) | 2014-09-02 | 2018-10-12 | Dna Script | Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques |
| EP3699283A1 (en) | 2014-10-20 | 2020-08-26 | Molecular Assemblies Inc. | Modified template-independent enzymes for polydeoxynucleotide systhesis |
| GB201502152D0 (en) | 2015-02-10 | 2015-03-25 | Nuclera Nucleics Ltd | Novel use |
| GB201503534D0 (en) | 2015-03-03 | 2015-04-15 | Nuclera Nucleics Ltd | Novel method |
| US11274333B2 (en) * | 2015-05-29 | 2022-03-15 | Molecular Cloning Laboratories (MCLAB) LLC | Compositions and methods for preparing sequencing libraries |
| GB201512372D0 (en) | 2015-07-15 | 2015-08-19 | Nuclera Nucleics Ltd | Novel method |
| GB2559117B (en) | 2017-01-19 | 2019-11-27 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method, kit and system |
| EP3737755B1 (en) | 2018-01-12 | 2024-08-28 | Camena Bioscience Limited | Method for template-free geometric enzymatic nucleic acid synthesis |
| GB201801768D0 (en) | 2018-02-02 | 2018-03-21 | Oxford Nanopore Tech Ltd | Synthesis method |
| GB2574197B (en) | 2018-05-23 | 2022-01-05 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method and system. |
| GB201811811D0 (en) | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
| GB201811813D0 (en) | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
| GB201811810D0 (en) | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
| GB201913039D0 (en) | 2019-09-10 | 2019-10-23 | Oxford Nanopore Tech Ltd | Polynicleotide synthesis method kit and system |
-
2017
- 2017-01-19 GB GB1700937.4A patent/GB2559117B/en active Active
-
2018
- 2018-01-19 AU AU2018209365A patent/AU2018209365B2/en active Active
- 2018-01-19 EP EP21182734.0A patent/EP3950115A1/en active Pending
- 2018-01-19 SG SG11201906553TA patent/SG11201906553TA/en unknown
- 2018-01-19 US US16/479,141 patent/US10927394B2/en active Active
- 2018-01-19 CA CA3050822A patent/CA3050822C/en active Active
- 2018-01-19 KR KR1020197024051A patent/KR102618717B1/ko active Active
- 2018-01-19 WO PCT/GB2018/050165 patent/WO2018134616A1/en not_active Ceased
- 2018-01-19 JP JP2019539789A patent/JP7295021B2/ja active Active
- 2018-01-19 CN CN201880017573.1A patent/CN110461463B/zh active Active
- 2018-01-19 EP EP18701553.2A patent/EP3570972B1/en active Active
-
2021
- 2021-01-07 US US17/143,318 patent/US11884950B2/en active Active
-
2023
- 2023-11-30 US US18/524,159 patent/US20240318219A1/en active Pending
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