JP2019514417A5 - - Google Patents
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- JP2019514417A5 JP2019514417A5 JP2018559814A JP2018559814A JP2019514417A5 JP 2019514417 A5 JP2019514417 A5 JP 2019514417A5 JP 2018559814 A JP2018559814 A JP 2018559814A JP 2018559814 A JP2018559814 A JP 2018559814A JP 2019514417 A5 JP2019514417 A5 JP 2019514417A5
- Authority
- JP
- Japan
- Prior art keywords
- mutgs
- nucleic acid
- expression system
- vector
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021178542A JP7344949B2 (ja) | 2016-05-11 | 2021-11-01 | グルタミンシンセターゼ遺伝子内相補ベクターを用いた高レベルのヘテロマータンパク質発現細胞の直接選択 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662334966P | 2016-05-11 | 2016-05-11 | |
| US62/334,966 | 2016-05-11 | ||
| PCT/US2017/032139 WO2017197098A1 (en) | 2016-05-11 | 2017-05-11 | Direct selection of cells expressing high levels of heteromeric proteins using glutamine synthetase intragenic complementation vectors |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021178542A Division JP7344949B2 (ja) | 2016-05-11 | 2021-11-01 | グルタミンシンセターゼ遺伝子内相補ベクターを用いた高レベルのヘテロマータンパク質発現細胞の直接選択 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019514417A JP2019514417A (ja) | 2019-06-06 |
| JP2019514417A5 true JP2019514417A5 (https=) | 2020-06-18 |
| JP6972022B2 JP6972022B2 (ja) | 2021-11-24 |
Family
ID=58873869
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018559814A Active JP6972022B2 (ja) | 2016-05-11 | 2017-05-11 | グルタミンシンセターゼ遺伝子内相補ベクターを用いた高レベルのヘテロマータンパク質発現細胞の直接選択 |
| JP2021178542A Active JP7344949B2 (ja) | 2016-05-11 | 2021-11-01 | グルタミンシンセターゼ遺伝子内相補ベクターを用いた高レベルのヘテロマータンパク質発現細胞の直接選択 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021178542A Active JP7344949B2 (ja) | 2016-05-11 | 2021-11-01 | グルタミンシンセターゼ遺伝子内相補ベクターを用いた高レベルのヘテロマータンパク質発現細胞の直接選択 |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US11384140B2 (https=) |
| EP (2) | EP3455359B1 (https=) |
| JP (2) | JP6972022B2 (https=) |
| KR (2) | KR102439719B1 (https=) |
| CN (2) | CN115873904A (https=) |
| AU (2) | AU2017263454B2 (https=) |
| BR (1) | BR112018073316A2 (https=) |
| CA (1) | CA3024027A1 (https=) |
| CL (1) | CL2018003210A1 (https=) |
| EA (1) | EA201892588A1 (https=) |
| ES (1) | ES2914118T3 (https=) |
| IL (2) | IL300591A (https=) |
| MA (1) | MA44976A (https=) |
| MX (2) | MX394682B (https=) |
| SG (1) | SG11201810040WA (https=) |
| TW (2) | TWI757291B (https=) |
| WO (1) | WO2017197098A1 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018093331A1 (en) * | 2016-11-16 | 2018-05-24 | Agency For Science, Technology And Research | Attenuated glutamine synthetase as a selection marker |
| MX2019014027A (es) | 2017-05-24 | 2020-08-03 | Thoeris Gmbh | Uso de glutamina sintetasa para tratar hiperamonemia. |
| CN109988777A (zh) * | 2017-12-29 | 2019-07-09 | 南京金斯瑞生物科技有限公司 | 谷氨酰胺合成酶基因以及应用 |
| AU2020269597A1 (en) * | 2019-05-07 | 2021-09-23 | Amgen Inc. | Vectors and expression systems for producing recombinant proteins |
| US20250101402A1 (en) * | 2021-08-03 | 2025-03-27 | Shanghai Zhenge Biotechnology Co., Ltd. | Selectable markers for eukaryotic expression system |
| WO2023125389A1 (en) * | 2021-12-27 | 2023-07-06 | Shanghai Zhenge Biotechnology Co., Ltd. | Novel markers for recombinant production system |
| JP2025510718A (ja) | 2022-03-23 | 2025-04-15 | ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング | 哺乳動物細胞における選択マーカーとしての細菌グルタミン合成酵素 |
| AU2023237597A1 (en) | 2022-03-23 | 2024-10-31 | Boehringer Ingelheim International Gmbh | New glutamine synthetase variants as selection marker |
| WO2025037708A1 (en) * | 2023-08-16 | 2025-02-20 | Korea Advanced Institute Of Science And Technology | Method for selection of cell line expressing high levels of recombinant protein using glutamine synthetase split expression vector |
| WO2025128343A1 (en) | 2023-12-11 | 2025-06-19 | Just-Evotec Biologics, Inc. | Protein expression using trans-splicing and split selectable markers |
| CN118006685B (zh) * | 2024-04-07 | 2024-07-23 | 上海碧博生物医药科技有限公司 | 一种快速的高表达单克隆细胞株构建方法 |
| KR20250169382A (ko) * | 2024-05-23 | 2025-12-03 | 한국과학기술원 | 디히드로엽산 환원효소 분할 발현벡터를 이용한 재조합 단백질 고발현 세포주의 선별방법 |
| WO2025250814A1 (en) * | 2024-05-31 | 2025-12-04 | Amgen Inc. | Intein based split glutamine synthetase engineering |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8308235D0 (en) | 1983-03-25 | 1983-05-05 | Celltech Ltd | Polypeptides |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| GB8422238D0 (en) | 1984-09-03 | 1984-10-10 | Neuberger M S | Chimeric proteins |
| GB8607679D0 (en) | 1986-03-27 | 1986-04-30 | Winter G P | Recombinant dna product |
| US5225539A (en) | 1986-03-27 | 1993-07-06 | Medical Research Council | Recombinant altered antibodies and methods of making altered antibodies |
| JPH0819508B2 (ja) | 1987-08-19 | 1996-02-28 | 三菱マテリアル株式会社 | Ｆｅ−Ｃｏ基合金製高周波用磁芯材 |
| IL162181A (en) | 1988-12-28 | 2006-04-10 | Pdl Biopharma Inc | A method of producing humanized immunoglubulin, and polynucleotides encoding the same |
| GB9014932D0 (en) | 1990-07-05 | 1990-08-22 | Celltech Ltd | Recombinant dna product and method |
| EP0519596B1 (en) | 1991-05-17 | 2005-02-23 | Merck & Co. Inc. | A method for reducing the immunogenicity of antibody variable domains |
| WO1994004679A1 (en) | 1991-06-14 | 1994-03-03 | Genentech, Inc. | Method for making humanized antibodies |
| US5624818A (en) | 1991-09-09 | 1997-04-29 | Fred Hutchinson Cancer Research Center | Nucleic acids encoding regulatory proteins that dimerize with Mad or Max |
| ES2198025T3 (es) | 1991-10-25 | 2004-01-16 | Immunex Corporation | Anticuerpos contra cd40-l. |
| JPH05244982A (ja) | 1991-12-06 | 1993-09-24 | Sumitomo Chem Co Ltd | 擬人化ｂ−ｂ１０ |
| US5573925A (en) | 1994-11-28 | 1996-11-12 | The Wistar Institute Of Anatomy And Biology | P53 proteins with altered tetramerization domains |
| IL122174A (en) | 1995-06-07 | 2004-07-25 | Immunex Corp | Dna encoding a cd40l mutein and a cd40l polypeptide encoded by said dna |
| US5916771A (en) | 1996-10-11 | 1999-06-29 | Abgenix, Inc. | Production of a multimeric protein by cell fusion method |
| US6342345B1 (en) | 1997-04-02 | 2002-01-29 | The Board Of Trustees Of The Leland Stanford Junior University | Detection of molecular interactions by reporter subunit complementation |
| GB9722131D0 (en) | 1997-10-20 | 1997-12-17 | Medical Res Council | Method |
| ES2257303T3 (es) * | 1999-07-12 | 2006-08-01 | Genentech, Inc. | Vectores de expresion y procedimientos. |
| GB9928787D0 (en) | 1999-12-03 | 2000-02-02 | Medical Res Council | Direct screening method |
| GB0025144D0 (en) | 2000-10-13 | 2000-11-29 | Medical Res Council | Concatenated nucleic acid sequences |
| WO2003035887A1 (en) | 2001-09-20 | 2003-05-01 | Immunex Corporation | Selection of cells expressing heteromeric polypeptides |
| GB0200977D0 (en) | 2002-01-17 | 2002-03-06 | Lonza Biologics Plc | Glutamine-auxotrophic human cells capable of producing proteins and capable of growing in a glutamine-free medium |
| AU2003298606A1 (en) * | 2002-10-25 | 2004-05-13 | Five Prime Therapeutics, Inc. | Human polypeptides encoded by polynucleotides and methods of their use |
| US7384744B2 (en) * | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| US20040202995A1 (en) | 2003-04-09 | 2004-10-14 | Domantis | Nucleic acids, proteins, and screening methods |
| JP4751326B2 (ja) | 2003-09-04 | 2011-08-17 | メダレックス インコーポレイテッド | 発現ベクター |
| WO2006036834A2 (en) | 2004-09-24 | 2006-04-06 | Amgen Inc. | MODIFIED Fc MOLECULES |
| US8053238B2 (en) | 2005-10-31 | 2011-11-08 | Unhwa Corporation | Isolated population of plant single cells and method of preparing the same |
| US20070254338A1 (en) * | 2006-04-24 | 2007-11-01 | Amgen Inc. | Method for making recombinant protein using complementation dependent DHFR mutants |
| WO2008154014A2 (en) | 2007-06-11 | 2008-12-18 | Amgen Inc. | A method for culturing mammalian cells to improve recombinant protein production |
| US9234210B2 (en) * | 2010-08-25 | 2016-01-12 | Intrexon Ceu, Inc. | Selectable markers and related methods |
| CN103201382A (zh) | 2010-11-08 | 2013-07-10 | 日本化学研究株式会社 | 新表达载体 |
| WO2012095514A1 (en) * | 2011-01-14 | 2012-07-19 | Vivalis | Recombinant protein production system |
| WO2012145682A1 (en) | 2011-04-21 | 2012-10-26 | Amgen Inc. | A method for culturing mammalian cells to improve recombinant protein production |
| US8809017B2 (en) * | 2011-05-24 | 2014-08-19 | Agency For Science, Technology And Research | IRES mediated multicistronic vectors |
| KR101411740B1 (ko) * | 2012-05-29 | 2014-06-27 | 한화케미칼 주식회사 | 항체 발현용 바이시스트로닉 발현벡터 및 이를 이용한 항체의 생산 방법 |
-
2017
- 2017-05-11 JP JP2018559814A patent/JP6972022B2/ja active Active
- 2017-05-11 AU AU2017263454A patent/AU2017263454B2/en active Active
- 2017-05-11 ES ES17727027T patent/ES2914118T3/es active Active
- 2017-05-11 TW TW106115604A patent/TWI757291B/zh not_active IP Right Cessation
- 2017-05-11 IL IL300591A patent/IL300591A/en unknown
- 2017-05-11 MX MX2018013882A patent/MX394682B/es unknown
- 2017-05-11 KR KR1020217035841A patent/KR102439719B1/ko active Active
- 2017-05-11 EP EP17727027.9A patent/EP3455359B1/en active Active
- 2017-05-11 BR BR112018073316A patent/BR112018073316A2/pt not_active Application Discontinuation
- 2017-05-11 US US16/099,986 patent/US11384140B2/en active Active
- 2017-05-11 SG SG11201810040WA patent/SG11201810040WA/en unknown
- 2017-05-11 KR KR1020187035185A patent/KR102323519B1/ko not_active Expired - Fee Related
- 2017-05-11 CN CN202211654230.0A patent/CN115873904A/zh active Pending
- 2017-05-11 CN CN201780034550.7A patent/CN109219660B/zh not_active Expired - Fee Related
- 2017-05-11 CA CA3024027A patent/CA3024027A1/en active Pending
- 2017-05-11 WO PCT/US2017/032139 patent/WO2017197098A1/en not_active Ceased
- 2017-05-11 EP EP22159835.2A patent/EP4067493A1/en active Pending
- 2017-05-11 MA MA044976A patent/MA44976A/fr unknown
- 2017-05-11 TW TW111105103A patent/TWI821905B/zh not_active IP Right Cessation
- 2017-05-11 EA EA201892588A patent/EA201892588A1/ru unknown
-
2018
- 2018-11-11 IL IL262923A patent/IL262923B2/en unknown
- 2018-11-12 CL CL2018003210A patent/CL2018003210A1/es unknown
- 2018-11-12 MX MX2022009674A patent/MX2022009674A/es unknown
-
2021
- 2021-11-01 JP JP2021178542A patent/JP7344949B2/ja active Active
-
2022
- 2022-05-26 US US17/825,793 patent/US12516111B2/en active Active
-
2023
- 2023-02-08 AU AU2023200660A patent/AU2023200660B2/en active Active
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