TWI757291B - 使用麩醯胺合成酶基因內互補載體直接選擇表現高水準異質蛋白的細胞 - Google Patents

使用麩醯胺合成酶基因內互補載體直接選擇表現高水準異質蛋白的細胞 Download PDF

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TWI757291B
TWI757291B TW106115604A TW106115604A TWI757291B TW I757291 B TWI757291 B TW I757291B TW 106115604 A TW106115604 A TW 106115604A TW 106115604 A TW106115604 A TW 106115604A TW I757291 B TWI757291 B TW I757291B
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mutgs
nucleic acid
seq
vector
polypeptide
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TW201805422A (zh
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藍朵 羅伯特 凱區
傑佛瑞 T 麥葛魯
雅德琳 迪娜 A 弗密娜
川特 菲利浦 穆羅
尼拉吉 加格底許 阿格沃
克麗斯汀 瑪莉 達瑞斯
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美商安美基公司
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07—ORGANIC CHEMISTRY
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    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)
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    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
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    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04004—Adenylosuccinate synthase (6.3.4.4)
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    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04005—Argininosuccinate synthase (6.3.4.5)
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles

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  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
TW106115604A 2016-05-11 2017-05-11 使用麩醯胺合成酶基因內互補載體直接選擇表現高水準異質蛋白的細胞 TWI757291B (zh)

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US201662334966P 2016-05-11 2016-05-11
US62/334,966 2016-05-11

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TW111105103A TWI821905B (zh) 2016-05-11 2017-05-11 使用麩醯胺合成酶基因內互補載體直接選擇表現高水準異質蛋白的細胞

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EP (2) EP3455359B1 (https=)
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CN (2) CN115873904A (https=)
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AU2020269597A1 (en) * 2019-05-07 2021-09-23 Amgen Inc. Vectors and expression systems for producing recombinant proteins
US20250101402A1 (en) * 2021-08-03 2025-03-27 Shanghai Zhenge Biotechnology Co., Ltd. Selectable markers for eukaryotic expression system
WO2023125389A1 (en) * 2021-12-27 2023-07-06 Shanghai Zhenge Biotechnology Co., Ltd. Novel markers for recombinant production system
JP2025510718A (ja) 2022-03-23 2025-04-15 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング 哺乳動物細胞における選択マーカーとしての細菌グルタミン合成酵素
AU2023237597A1 (en) 2022-03-23 2024-10-31 Boehringer Ingelheim International Gmbh New glutamine synthetase variants as selection marker
WO2025037708A1 (en) * 2023-08-16 2025-02-20 Korea Advanced Institute Of Science And Technology Method for selection of cell line expressing high levels of recombinant protein using glutamine synthetase split expression vector
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CN118006685B (zh) * 2024-04-07 2024-07-23 上海碧博生物医药科技有限公司 一种快速的高表达单克隆细胞株构建方法
KR20250169382A (ko) * 2024-05-23 2025-12-03 한국과학기술원 디히드로엽산 환원효소 분할 발현벡터를 이용한 재조합 단백질 고발현 세포주의 선별방법
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SG11201810040WA (en) 2018-12-28
MX394682B (es) 2025-03-24
KR102439719B1 (ko) 2022-09-02
CA3024027A1 (en) 2017-11-16
IL300591A (en) 2023-04-01
IL262923A (en) 2019-01-31
KR102323519B1 (ko) 2021-11-09
ES2914118T3 (es) 2022-06-07
WO2017197098A1 (en) 2017-11-16
AU2017263454B2 (en) 2023-02-09
US20230048658A1 (en) 2023-02-16
AU2017263454A1 (en) 2018-12-06
TWI821905B (zh) 2023-11-11
TW202223093A (zh) 2022-06-16
CN109219660A (zh) 2019-01-15
EP3455359B1 (en) 2022-03-09
MX2022009674A (es) 2022-09-09
US11384140B2 (en) 2022-07-12
CN115873904A (zh) 2023-03-31
TW201805422A (zh) 2018-02-16
US20190127452A1 (en) 2019-05-02
CN109219660B (zh) 2023-01-06
AU2023200660B2 (en) 2024-12-12
IL262923B2 (en) 2023-08-01
JP7344949B2 (ja) 2023-09-14
MX2018013882A (es) 2019-03-14
JP2022033734A (ja) 2022-03-02
KR20210135635A (ko) 2021-11-15
US12516111B2 (en) 2026-01-06
JP2019514417A (ja) 2019-06-06
JP6972022B2 (ja) 2021-11-24
AU2023200660A1 (en) 2023-03-09
BR112018073316A2 (pt) 2019-06-04
MA44976A (fr) 2019-03-20
IL262923B1 (en) 2023-04-01
EP4067493A1 (en) 2022-10-05
CL2018003210A1 (es) 2019-02-15
KR20190005193A (ko) 2019-01-15
EA201892588A1 (ru) 2019-06-28
EP3455359A1 (en) 2019-03-20

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