JP2019514395A5 - - Google Patents

Info

Publication number

JP2019514395A5

JP2019514395A5 JP2018557402A JP2018557402A JP2019514395A5 JP 2019514395 A5 JP2019514395 A5 JP 2019514395A5 JP 2018557402 A JP2018557402 A JP 2018557402A JP 2018557402 A JP2018557402 A JP 2018557402A JP 2019514395 A5 JP2019514395 A5 JP 2019514395A5

Authority

JP

Japan

Prior art keywords

acylase

penicillin

engineered

seq

insulin

Prior art date

2017-05-05

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
• Discuss

• 108010073038 Penicillin Amidase Proteins 0.000 claims description 138
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 138
• 102000004877 Insulin Human genes 0.000 claims description 69
• 108090001061 Insulin Proteins 0.000 claims description 69
• 229940125396 insulin Drugs 0.000 claims description 69
• 238000000034 method Methods 0.000 claims description 44
• 238000006467 substitution reaction Methods 0.000 claims description 37
• IPBVNPXQWQGGJP-UHFFFAOYSA-N acetic acid phenyl ester Natural products CC(=O)OC1=CC=CC=C1 IPBVNPXQWQGGJP-UHFFFAOYSA-N 0.000 claims description 32
• 229940049953 phenylacetate Drugs 0.000 claims description 32
• WLJVXDMOQOGPHL-UHFFFAOYSA-N phenylacetic acid Chemical compound OC(=O)CC1=CC=CC=C1 WLJVXDMOQOGPHL-UHFFFAOYSA-N 0.000 claims description 32
• 229920001184 polypeptide Polymers 0.000 claims description 29
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 29
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 29
• 102000040430 polynucleotide Human genes 0.000 claims description 15
• 108091033319 polynucleotide Proteins 0.000 claims description 15
• 239000002157 polynucleotide Substances 0.000 claims description 15
• 238000004519 manufacturing process Methods 0.000 claims description 14
• 210000004027 cell Anatomy 0.000 claims description 12
• 150000001413 amino acids Chemical class 0.000 claims description 10
• DCYGAPKNVCQNOE-UHFFFAOYSA-N 2,2,2-triphenylacetic acid Chemical group C=1C=CC=CC=1C(C=1C=CC=CC=1)(C(=O)O)C1=CC=CC=C1 DCYGAPKNVCQNOE-UHFFFAOYSA-N 0.000 claims description 8
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 6
• 241000588724 Escherichia coli Species 0.000 claims description 2
• 238000012258 culturing Methods 0.000 claims description 2
• 210000003527 eukaryotic cell Anatomy 0.000 claims description 2
• 210000001236 prokaryotic cell Anatomy 0.000 claims description 2
• 125000003275 alpha amino acid group Chemical group 0.000 claims 11
• 230000001681 protective effect Effects 0.000 claims 3