MX389364B - Penicilina g acilasas - Google Patents

Penicilina g acilasas

Info

Publication number
MX389364B
MX389364B MX2018013259A MX2018013259A MX389364B MX 389364 B MX389364 B MX 389364B MX 2018013259 A MX2018013259 A MX 2018013259A MX 2018013259 A MX2018013259 A MX 2018013259A MX 389364 B MX389364 B MX 389364B
Authority
MX
Mexico
Prior art keywords
enzymes
penicillin
acylases
acylase
pga
Prior art date
Application number
MX2018013259A
Other languages
English (en)
Other versions
MX2018013259A (es
Inventor
Courtney Dianne Moffett
David Elgart
Jovana Nazor
Judy Victoria Antonio Viduya
Katrina W Lexa
Nikki Dellas
Oscar Alvizo
Ravi David Garcia
Robert Kevin Orr
Vesna Mitchell
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of MX2018013259A publication Critical patent/MX2018013259A/es
Publication of MX389364B publication Critical patent/MX389364B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01011—Penicillin amidase (3.5.1.11), i.e. penicillin-amidohydrolase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La presente invención se refiere a una penicilina G acilasa diseñada caracterizada porque tiene una secuencia de polipéptido que es por lo menos 85%, 90%, 91%, 92%, 93%, 94% 95%, 96% , 97%, 98% 99% o más idéntica a la SEQ ID NO: 1036, y comprende una mutación N2L,en donde dicha posición está numerada con referencia a la SEQ ID NO : 1036, y en donde dicha penicilina G acilasa es capaz de producir insulina mono-protegida o di-protegida de acetato de fenilo y tiene una mejora en la actividad enzimática de al menos 1.5 veces a la actividad enzimática de la SEQ ID NO: 1036.
MX2018013259A 2016-05-05 2017-05-05 Penicilina g acilasas MX389364B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662332103P 2016-05-05 2016-05-05
PCT/US2017/031342 WO2017193022A1 (en) 2016-05-05 2017-05-05 Penicillin-g acylases

Publications (2)

Publication Number Publication Date
MX2018013259A MX2018013259A (es) 2019-04-22
MX389364B true MX389364B (es) 2025-03-19

Family

ID=60203339

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018013259A MX389364B (es) 2016-05-05 2017-05-05 Penicilina g acilasas

Country Status (12)

Country Link
US (4) US10724025B2 (es)
EP (1) EP3452587B1 (es)
JP (1) JP7128519B2 (es)
KR (1) KR102382489B1 (es)
CN (1) CN109715793A (es)
AU (1) AU2017260355B2 (es)
CA (1) CA3021009A1 (es)
DK (1) DK3452587T3 (es)
IL (1) IL262550B (es)
MX (1) MX389364B (es)
SG (1) SG11201808957UA (es)
WO (1) WO2017193022A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017193022A1 (en) 2016-05-05 2017-11-09 Codexis, Inc. Penicillin-g acylases
JP7244089B2 (ja) 2017-06-27 2023-03-22 コデクシス， インコーポレイテッド ペニシリンｇアシラーゼ
JP2022547523A (ja) * 2019-09-12 2022-11-14 コデクシス， インコーポレイテッド １０－アセチル－３，７－ジヒドロキシフェノキサジンに対するペルオキシダーゼ活性

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Also Published As

Publication number Publication date
DK3452587T3 (da) 2021-10-11
JP7128519B2 (ja) 2022-08-31
EP3452587A4 (en) 2020-03-18
KR102382489B1 (ko) 2022-04-01
NZ747064A (en) 2025-05-02
MX2018013259A (es) 2019-04-22
US11214786B2 (en) 2022-01-04
US20170321202A1 (en) 2017-11-09
IL262550B (en) 2022-05-01
EP3452587B1 (en) 2021-09-08
US10724025B2 (en) 2020-07-28
AU2017260355A1 (en) 2018-11-01
KR20180137025A (ko) 2018-12-26
US11965192B2 (en) 2024-04-23
US20220154164A1 (en) 2022-05-19
WO2017193022A1 (en) 2017-11-09
JP2019514395A (ja) 2019-06-06
US20200339972A1 (en) 2020-10-29
SG11201808957UA (en) 2018-11-29
CN109715793A (zh) 2019-05-03
EP3452587A1 (en) 2019-03-13
IL262550A (en) 2018-12-31
AU2017260355B2 (en) 2023-03-30
CA3021009A1 (en) 2017-11-09
US20250034543A1 (en) 2025-01-30

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