KR20180137025A - 페니실린 ｇ 아실라제 - Google Patents

Abstract

본 발명은 개선된 특성을 가지는 조작된 페니실린 G 아실라제(PGA) 효소, 상기 효소를 코딩하는 폴리뉴클레오티드, 상기 효소를 포함하는 조성물, 및 상기 효소를 사용하는 방법을 제공한다.

Description

페니실린 Ｇ 아실라제

본 출원은 2016년 5월 6일 출원된 미국 가특허 출원 제62/332,103호에 대한 우선권을 주장하며, 상기 출원은 그 전문이 모든 목적을 위해 본원에서 참조로 포함된다.

본 발명의 분야

본 발명은 조작된 페니실린 G 아실라제(PGA: penicillin G acylase) 효소, 상기 효소를 코딩하는 폴리뉴클레오티드, 상기 효소를 포함하는 조성물, 및 조작된 PGA 효소를 사용하는 방법을 제공한다.

서열 목록, 표 또는 컴퓨터 프로그램에 관한 참조

파일명이 "CX2-154WO1_ST25.txt"이고, 작성일이 2017년 5월 5일이며, 크기가 8,962 킬로바이트 인 서열 목록의 공식 사본은 ASCII 포맷된 텍스트 파일로서 EFS-웹을 통해 본 명세서와 함께 동시에 제출된 것이다. EFS-웹을 통해 제출된 서열 목록은 본 명세서의 일부이며, 이는 그 전문이 본원에서 참조로 포함된다.

페니실린 G 아실라제(PGA)(페니실린 아미다제, EC 3.5.1.11)는 페니실린 G(벤질페니실린) 측쇄의 아미드 결합의 절단을 촉매화한다. 상기 효소는 6-아미노-페니실란산(6-APA: 6-amino-penicillanic acid) 및 페닐-아세트산(PAA: phenyl-acetic acid)의 제조에서 상업적으로 사용된다. 6-APA는 반합성 β 락탐 항생제, 예컨대, 아목시실린, 암피실린 및 세팔렉신의 산업적 생산에서 핵심 화합물이다. 자연적으로 발생된 PGA 효소는 상업적 프로세스에서 불안정성을 보이는 바, 상업적 적용을 위해서는 고체 기판 상에의 고정화가 요구된다. PGA는 다양한 지지체에 공유적으로 결합되었고, PGA 고정화된 시스템은 순수한 광학 이성질체의 합성을 위한 유용한 도구로서 보고되었다. 그러나, 고체 표면에의 부착은 예컨대, 활성 및/또는 선택성 감소, 및 용질 접근의 제한과 같이, 효소 특성을 손상시킨다. 더욱이, 비록 고체 기판에의 부착을 통해 효소가 포획될 수 있고, 추가의 프로세싱 사이클에서 재사용이 가능하지만, 효소의 안정성은 그 적용이 제한될 수 있는 정도이다. 페니실린 G에서 6-APA로의 PGA에 의한 효소적 촉매 작용은 위치특이적이고(락탐 아미드 결합은 절단하지 않음), 입체특이적이다. 6-APA 생산은 아마도 의약 생산에서 효소적 촉매 작용을 최대로 이용하는 것으로 여겨진다. 펜아세틸 모이어티와 회합된, PGA의 효소 활성은 알콜 뿐만 아니라, 1급 아민의 매우 다양한 펜아세틸 유도체의 입체특이적 가수분해를 가능하게 한다.

본 발명은 조작된 페니실린 G 아실라제(PGA) 효소, 상기 효소를 코딩하는 폴리뉴클레오티드, 상기 효소를 포함하는 조성물, 및 조작된 PGA 효소를 사용하는 방법을 제공한다.

본 발명은 유리 인슐린의 A1, B1 또는 B29 위치에 보호기를 부가함으로써, 또는 A1/B1/B29 삼중 보호된 인슐린으로부터 보호기를 제거함으로써 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 생산할 수 있는 조작된 페니실린 G 아실라제를 제공한다. 일부 실시양태에서, 페니실린 G 아실라제는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288과 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99% 또는 그 이상 동일하다. 일부 실시양태에서, 본 발명은 인슐린으로부터 A1, B1, 또는 B29 트리-페닐 아세테이트 보호기를 제거하여 디-페닐 아세테이트 보호된 인슐린을 생산할 수 있는 조작된 페니실린 G 아실라제로서, 여기서, 페니실린 G 아실라제는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288과 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 것인, 조작된 페니실린 G 아실라제를 제공한다. 일부 실시양태에서, 페니실린 G 아실라제는 인슐린의 A1 트리-페닐 아세테이트 보호기를 제거하는 반면, 일부 추가의 실시양태에서, 페니실린 G 아실라제는 인슐린의 B1 트리-페닐 아세테이트 보호기를 제거하고, 또 다른 추가의 실시양태에서, 페니실린 G 아실라제는 인슐린의 B29 트리-페닐 아세테이트 보호기를 제거한다. 일부 실시양태에서, 페니실린 G 아실라제는 인슐린의 A1, B1, 및 B29 트리-페닐 아세테이트 보호기를 제거한다. 일부 추가의 실시양태에서, 조작된 페니실린 G 아실라제는, 서열 번호: 2의 폴리펩티드에 의한 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린의 생산과 비교하였을 때, 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 초과 또는 그 이상으로 생산한다.

일부 추가의 실시양태에서, 본 발명은 인슐린으로부터 A1/B1/B29 트리-페닐 아세테이트 보호기를 제거함으로써 유리 인슐린을 생산할 수 있는 조작된 페니실린 G 아실라제로서, 여기서, 페니실린 G 아실라제는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288를 포함하는 것인, 조작된 페니실린 G 아실라제를 제공한다. 일부 추가의 실시양태에서, 페니실린 G 아실라제는 표 5.1, 6.1, 7.1, 8.1, 8.2, 9,1, 10.1, 12.1, 12.2, 12.3, 12.4, 12.5, 12.6, 및/또는 12.7에 제공된 바와 같은 페니실린 G 변이체 중 적어도 하나의 돌연변이를 포함한다. 일부 실시양태에서, 본 발명은 표 5.1, 6.1, 7.1, 8.1, 8.2, 9,1, 10.1, 12.1, 12.2, 12.3, 12.4, 12.5, 12.6, 및/또는 12.7에 기재된 적어도 하나의 서열과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 서열을 포함하는 조작된 페니실린 G 아실라제를 제공한다. 일부 실시양태에서, 페니실린 G 아실라제는 표 5.1, 6.1, 7.1, 8.1, 8.2, 9,1, 10.1, 12.1, 12.2, 12.3, 12.4, 12.5, 12.6, 및/또는 12.7에 제공된 변이체를 포함한다.

본 발명은 또한, 서열 번호: 8과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 27, 27;28;71;74;547, 27;71;74;484;547;584;697, 71;74, 129, 253, 254, 256, 348, 352, 372, 373, 374, 380, 380;457, 386;390, 386;390, 386, 387;390, 451, 457, 467, 470, 474, 616, 623, 704, 706, 및 708로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 8을 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제를 제공한다.

본 발명은 또한, 서열 번호: 14와 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 9, 9;103;119;131;233;312;324;432;444;494, 9;103;119;131;324;432;494;646, 9;103;119;131;233;269;304;444;494;646, 9;103;119;131;304;324;432;444;494;646, 9;103;119;233;646, 9;103;119;494, 9;103;233;312;646, 9;103;233;432;646, 9;103;233;494, 9;103;269;304;324;494;646, 9;103;304;432;444;646, 9;119;131;233, 9;119;131;233;304;444;646, 9;119;131;233;494;646, 9;119;131;233;494;661, 9;119;131;312;444;646, 9;119;131;432;444;646, 9;119;233;269;273;304;312;432;444;646, 9;119;233;494;646, 9;119;304;444;494;646, 9;131;233;269;273;312;432;444;646, 9;233;273;304;494;646, 9;233;304;494;646, 9;233;312;432;646, 9;233;494;646, 9;312;444;646, 9;432;444;494;646, 9;494;646, 28;374;380, 103;119;131;233;273;304;324;432;444;494;646, 103;119;131;233;304;312;432;494;646;661, 103;119;131;269;312;494;646, 103;119;233, 103;119;233;273;432, 103;119;233;304;646, 103;119;233;312;646, 103;119;494;646;661, 103;119;646, 103;131;233;304;324;444;646, 103;131;269;273;444;646, 103;233;273;312;324;432;444;646, 103;233;273;312;432;444;646, 103;269;273;444;646, 103;273;304;324;444;494, 103;312;444;646, 103;444;494;646, 119;131;444;646;661, 119;131;494, 119;131;646, 119;131;304;432;444;646;661, 119;131;444, 119;233;304;312;324;432;444;646, 119;233;304;646, 119;233;312, 119;233;646, 119;269;273;312;324;494;646, 119;269;273;312;432;444;646, 119;273;324;444;494;646, 119;312;444;646, 119;432;444;646, 129;254;348;457;704, 129;348, 129;348;467;470;704;708, 129;348;470;623;704;706, 129;348;470;623;704;706;708, 129;380;470, 129;457;470;474, 129;470,129;623, 131;233;273;646, 131;233;304;444, 131;233;432;646, 131;273;432;444;494;646, 185, 233;269;304;312;324;432;444;646, 253, 253;256, 253;256;352;373;374;616, 253;256;352;374;380;451, 253;256;374;451, 253;256;380;451;616, 253;352;374;616, 253;352;451;616, 253;373;451, 253;374;451, 253;374;451;623, 253;451, 253;451;457, 253;254;352;374;380, 253;256, 253;256;352;374;451, 253;256;352;380;451;616, 253;256;352;451, 253;256;352;374;380;451, 253;256;352;380, 253;256;373, 253;256;374;616, 253;256;380, 253;256;380;451;546;616, 253;352;373;374;451, 253;352;373;374;451;616, 253;352;374;616, 253;352;623, 253;373;374;451;616, 253;380, 254;255;352, 254;256;352;451, 254;256;373;374;380;451, 254;256;374, 254;256;374;451, 254;256;451, 254;352;380, 254;256;380;451;616, 254;352;380;451, 254;352;451, 254;373;374;380;451, 254;373;374;451, 254;374, 254;374;380, 254;380, 254;451, 254;616, 256;352;374, 256;352;380;451;616, 256;374;380;451;616, 256;374;616, 273;312;444;646, 304;312;444;646, 312;444;646, 312;646, 348, 348;372;470;623;708, 348;704;708, 352, 352;373;374;451, 352;373;380;451, 352;373;380;451;616, 352;373;451, 352;374, 352;374;380, 352;374;380;451;616, 352;374;451, 352;374;616, 352;380, 352;380;451, 352;380;451;616, 352;380;451;623, 352;380;616, 352;451, 352;451;616, 352;616, 372;457;470;623, 373;374, 373;374;451, 373;451, 373;616, 374, 374;380, 374;380;451, 374;380;451;623, 374;451, 374;451;616, 374;616, 374;623, 380, 380;451, 380;451;616, 380;451;623, 380;616, 380;616;623, 380;623, 415, 443, 444, 444;494, 444;646, 451, 451;616, 451;623, 457, 457;470, 457;704;708, 470;708, 492;493, 517, 560, 616, 623, 723, 및 748로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 14를 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제를 제공한다.

본 발명은 또한, 서열 번호: 300과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 9;61;444, 9;168;185;517;560;748, 9;185;415, 9;185;415;443;444;517;723;748, 9;185;415;443;444;517;560, 9;185;415;443;444;517;748, 9;185;415;444;517;723;748, 9;185;415;444;517;748, 9;185;415;444;517;560, 9;185;415;444;517;560;723;748, 9;185;415;444, 9;185;415;444;560, 9;185;415;444;723;748, 9;185;415;444;517;560;723;748, 9;185;415;517;560, 9;185;415;517;723, 9;185;415;517;748, 9;185;415;748, 9;185;443;444;517;560, 9;185;443;444;723, 9;185;443;444;560;723, 9;185;443;444;517, 9;185;444, 9;185;444;517;560, 9;185;444;560;723, 9;185;444;560;748, 9;185;444;517;560, 9;185;444;517;560;723, 9;185;444;517;560;748, 9;185;444;517;723;748, 9;185;444;560, 9;185;444;723, 9;185;444;517;560;723, 9;185;517, 9;185;517;560;723;748, 9;185;748, 9;415;443;444;517;560, 9;415;443;444;517;748, 9;415;443;444;560;723;748, 9;415;443;444;517;560, 9;415;443;444;517;560;723, 9;415;443;444;517;748, 9;415;443;560, 9;415;443;560;723;748, 9;415;444, 9;415;444;517;560;748, 9;415;444;517, 9;415;444;517;560;723;748, 9;415;444;560;723;748, 9;415;444;723;748, 9;415;444;517, 9;415;444;517;560;723;748, 9;415;444;560;723, 9;415;444;517, 9;415;444;517;560;748, 9;415;444;517, 9;415;444;560, 9;415;444;560, 9;415;444;517;560;723, 9;415;444;560, 9;415;444;560;665;723;748, 9;415;444;723;748, 9;415;517, 9;415;517;560;723;748, 9;415;517;560;723;748, 9;415;517;560;748, 9;415;560, 9;415;748, 9;443;444;517;560;723, 9;443;444;560, 9;443;444;560;723;748, 9;443;444;517, 9;443;444;560;723, 9;443;444;517, 9;443;444;517;560, 9;443;444;517;748, 9;443;517;748, 9;443;723, 9;444;517;560, 9;444;560;723, 9;444;748, 9;444;517;560;723;748, 9;444;560, 9;444;560;723, 9;444;560;748, 9;444;560;748, 9;444;748, 9;444;517;560;723;748, 9;444;517, 9;444;517;560;723, 9;444;517;723, 9;444;560;748, 9;444;723, 9;444, 9;444;517;560, 9;517, 9;517;560;723, 9;517;560;748, 9;517;560;748, 9;517;723, 9;517;748, 9;560;723;748, 9;723, 103, 103;119, 103;119;129;254;256;348;494;646, 103;119;129;444;494, 103;119;254;348,103;119;254;348;444, 103;119;254;444, 103;119;256;348;444;494;646, 103;119;256;494, 103;119;348, 103;119;348;457, 103;119;348;457;494, 103;119;457, 103;119;494, 103;119;494;646, 103;129, 103;129;254;444;457;494, 103;129;256;348, 103;129;348;646, 103;254, 103;254;256;348;444;494, 103;254;646,103;254;348, 103;254;348;494, 103;256, 103;256;444, 103;256;457, 103;256;494, 103;348, 103;348;444, 103;348;494, 103;444, 103;494, 103;494;646,119;129;254;348;494, 119;129;254;457;494, 119;129;256;348;457, 119;254;348;457, 119;256;348 119;256;348;494, 119;256;444, 119;348, 119;348;494, 185;415;443;444;517;560, 185;415;443;444;517, 185;415;444;517;560;748, 185;415;444;517;748, 185;415;444;560, 185;415;560, 185;415;560;723, 185;415;723;748, 185;443;444;560, 185;444;446;517;560, 185;444;517;560, 185;444;517;560;723;748, 185;444;517;723, 185;444;560, 185;517;560;723,185;560, 185;560;723, 185;560;748, 254;457, 256;348, 256;494, 348, 348;444, 348;444;646, 348;457, 348;494, 415, 415;443;444;517;748, 415;443;444;560, 415;443;517;560;723, 415;443;517;723, 415;444;723, 415;444;517, 415;444;517;560;723;748, 415;444;517;560;748, 415;444;560;723, 415;444;517;560;723, 415;444;517;723;748 415;517;560;723;748, 415;444;560, 415;444;560;748, 415;444, 415;517, 415;517;560, 415;517;560;748, 415;560;723, 415;723;748, 415;723;748, 415;748, 443;444;517;560;723;748, 443;444;517;748, 443;444, 443;444;560, 443;444;723, 443;517, 444, 444;517;560, 444;517;748, 444;748, 444;517;560;723;748, 444;560;723, 444;560, 444;560;723, 444;517, 444;517;560, 444;517;723;748, 444;560;748, 444;723, 444;517;560, 444;560;723, 517;560, 517;560;748, 517;723, 517;748, 517;748, 560, 723, 및 723;748로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 300을 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제를 제공한다.

본 발명은 또한, 서열 번호: 1262와 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 24, 24;27;28;701;729, 24;28;56;308;379;701, 24;28;56;701, 24;28;71;701, 24;28;321;701, 24;28;457;701, 24;31;56;386;701, 24;31, 24;31;56;697, 24;31;56;701, 24;31;56;264;701;750, 24;31;71;701, 24;56;154;270;697, 24;56;697;701, 24;56;701, 24;71;701, 24;225;701, 24;484;701, 24;28, 28;31, 31;56;701, 56;71;701, 56;119;146;701, 56;154;701, 56;322;697;701, 56;658;701, 56;697;701, 56;701,56;701;711, 697, 697;701, 71;74, 71;701, 129;511;701, 154;754, 177, 410;697;701, 423;701, 431, 697, 및 701로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1262를 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제를 제공한다.

본 발명은 또한, 서열 번호: 1288과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 22, 31, 31;56;264;308;379;484;547;711;750, 32, 50, 57, 69;74, 71, 71;74, 71;74;129, 71;74;145, 71;74;248, 71;74;470, 71;149, 75, 141, 및 394로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1288을 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제를 제공한다.

본 발명은 또한, 서열 번호: 1036과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 2, 47, 176, 253, 255, 384, 460, 467, 536, 및 623로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1036을 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제를 제공한다.

본 발명은 또한, 서열 번호: 1194와 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 12;103;119;131;233;384;444;494;S646, 12;103;119;131;233;444;494;S646, 12;103;119;233;384;444;494;S646, 12;103;119;233;444;467;494;S646, 12;103;119;233;444;494;536;S646, 12;103;119;233;444;494;S646, 12;103;131;233;444;467;494;S646, 12;103;131;233;444;494;S646, 12;103;233;444;494;S646, 12;119;233;384;444;494;S646, 28;264;384;467;484;536;547, 103;119;131;233;384;444;494;S646, 103;119;233;444;494;S646, 103;233;444;494;S646, 264;384;467;484;536;547, 384;467;484;536;547, 및 668로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1194를 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제를 제공한다.

본 발명은 또한, 서열 번호: 1288과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 20;709, 27, 27;74;253;254, 27;74;253;254;255;348;369;370;381, 27;74;253;254;255;348;370;384, 27;74;253;254;255;369;370, 27;74;253;254;255;370, 27;74;253;254;255;370;381;384, 27;74;253;254;255;381, 27;74;253;254;348, 27;74;253;254;384, 27;74;253;255, 27;74;253;255;348;370;384, 27;74;253;255;348;381, 27;74;253;255;348;384, 27;74;253;348;369;370, 27;74;253;348;369;370;381, 27;74;253;348;369;370;381;384, 27;74;253;348;370, 27;74;253;381;384, 27;74;253;384, 27;74;254;255;348, 27;74;254;255;348;369;370;381, 27;74;254;255;348;370, 27;74;254;255;348;381, 27;74;254;255;381, 27;74;254;348;381;384, 27;74;254;369;370, 27;74;255;348, 27;74;255;348;369;370, 27;74;255;348;369;381;384, 27;74;255;370, 27;74;348, 27;74;369;370, 27;74;107;255;348;369;370, 27;74;253, 27;74;253;254;255, 27;74;253;254;255;348;370, 27;74;253;254;348;369;370;381, 27;74;253;254;348;369;384, 27;74;253;254;348;370, 27;74;253;254;348;370;381, 27;74;253;254;369, 27;74;253;254;370;381, 27;74;253;255;348;369;370, 27;74;253;255;370, 27;74;253;348, 27;74;253;348;370;381;384, 27;74;253;369;381;384, 27;74;254,27;74;254;255;348;369;381, 27;74;254;255;348;370;381, 27;74;254;255;369, 27;74;254;348, 27;74;254;348;369;381;384, 27;74;254;348;370, 27;74;254;348;370;381, 27;74;254;384, 27;74;255;348;370, 27;74;348;384, 27;74;253;254;255;348;369;370, 27;74;253;254;348;369, 27;74;253;254;348;369;370, 27;74;253;254;348;370;381;384, 27;74;253;254;348;381;384, 27;74;253;370, 27;74;254;255;348;369;370, 27;74;254;255;348;381;384, 27;74;370, 27;74;253;254;255;348, 27;74;253;255;348;370;381, 27;74;253;255;384, 27;74;253;348;369;370;384, 27;74;253;348;381, 27;74;254;255;348;369;384, 27;74;254;255;348;370;381;384, 27;74;254;255;370, 27;74;254;348;381, 27;74;254;369;384, 27;74;255, 27;74;255;348;369;381, 27;74;348;370, 27;74;369;370;381;384, 27;253, 27;253;254, 27;253;254;255, 27;253;254;255;260;348;381;384, 27;253;254;255;348, 27;253;254;255;348;369;370;381;384, 27;253;254;255;348;369;384, 27;253;254;255;348;370, 27;253;254;255;348;370;384, 27;253;254;255;348;381;384, 27;253;254;348, 27;253;254;348;370;381, 27;253;254;348;370;384, 27;253;254;348;381, 27;253;254;348;381;384, 27;253;254;348;384, 27;253;254;381, 27;253;254;381;384, 27;253;254;384, 27;253;255;348, 27;253;255;348;369;370, 27;253;255;348;381, 27;253;255;348;384, 27;253;255;370, 27;253;255;370;381;384, 27;253;348, 27;253;348;370;381;384, 27;253;348;370;384, 27;253;348;381;384, 27;253;369;370, 27;253;381;384, 27;254;255, 27;254;255;348, 27;254;255;348;369;370, 27;254;255;348;370, 27;254;255;348;370;381, 27;254;255;348;370;381;384, 27;254;255;348;370;384, 27;254;255;369;370;381;384, 27;254;255;370, 27;254;255;381;384, 27;254;255;384, 27;254;348;369;370;381;384, 27;254;348;370, 27;254;348;381;384, 27;254;348;384, 27;254;369;381;384, 27;254;449, 27;254;470, 27;255;348, 27;255;348;370, 27;255;348;370;381;384, 27;255;348;381;384, 27;255;370, 27;348, 27;348;369;370;381;384, 27;348;381, 27;348;384, 69, 74;253;254;369;370, 74;254;255;348;384, 84, 128, 131, 132, 133, 134, 253, 253;348;370, 254, 255, 255;348;370, 256, 317;380, 348;467, 370, 373, 377, 381, 381;672, 383, 384, 388, 453, 457, 467, 472, 615, 616, 618, 619, 620, 623, 627, 701, 705, 706, 708, 및 709로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1288을 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제를 제공한다.

본 발명은 또한, 서열 번호: 1262와 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 24;31;56;701, 24;31;56;701, 24;31;71;701, 24;56;701, 및 71;74로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1262를 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제를 제공한다.

본 발명은 서열 번호: 3-1901 중 홀수 번호의 서열에 제공된 적어도 하나의 서열과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 서열을 포함하는 조작된 페니실린 G 아실라제를 제공한다.

본 발명은 본원에 제공된 적어도 하나의 페니실린 G 아실라제를 포함하는 조성물을 추가로 제공한다. 일부 실시양태에서, 조성물은 적어도 하나의 고정화된 페니실린 G 아실라제를 포함한다.

본 발명은 또한 서열 번호: 3, 7, 13, 299, 1035, 1193, 1261, 및/또는 1287로부터 선택되는 서열과 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99% 또는 그 이상의 서열 동일성을 가지는 폴리뉴클레오티드 서열에 의해 코딩된 페니실린 G 아실라제를 제공한다. 일부 실시양태에서, 페니실린 G 아실라제를 코딩하는 폴리뉴클레오티드 서열은 서열 번호: 3, 7, 13, 299, 1035, 1193, 1261, 및/또는 1287로부터 선택되는 서열과 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상의 서열 동일성을 가진다. 일부 실시양태에서, 폴리뉴클레오티드 서열은 서열 번호: 3, 7, 13, 299, 1035, 1193, 1261, 및/또는 1287로부터 선택된다. 일부 실시양태에서, 조작된 폴리뉴클레오티드 서열은 서열 번호: 3-1901 중 홀수 번호의 서열에 제공된 적어도 하나의 서열과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 서열을 포함한다.

본 발명은 또한 본원에 제공된 폴리뉴클레오티드 서열(예컨대, 서열 번호: 3, 7, 13, 299, 1035, 1193, 1261, 및/또는 1287)을 포함하는 벡터를 제공한다. 본 발명은 또한 본원에 제공된 벡터(예컨대, 서열 번호: 3, 7, 13, 299, 1035, 1193, 1261, 및/또는 1287의 폴리뉴클레오티드 서열을 포함하는 벡터)를 포함하는 숙주 세포를 제공한다. 일부 실시양태에서, 벡터는 서열 번호: 3-1901 중 홀수 번호의 서열에 제공된 적어도 하나의 서열과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 서열을 포함하는 적어도 하나의 조작된 폴리뉴클레오티드 서열을 포함한다.

본 발명은 본원에 제공된 벡터를 포함하는 숙주 세포를 제공한다. 일부 실시양태에서, 숙주 세포는 원핵생물 또는 진핵생물 세포이다. 일부 추가의 실시양태에서, 숙주 세포는 박테리아 세포이다. 일부 추가의 실시양태에서, 숙주 세포는 이. 콜라이(E. coli)이다.

본 발명은 본원에 제공된 적어도 하나의 조작된 페니실린 G 아실라제를 포함하는 조성물을 추가로 제공한다. 추가로, 본 발명은 조작된 페니실린 G 아실라제가 생산되는 조건하에서 본원에 제공된 숙주 세포를 배양하는 단계를 포함하는, 본원에 제공된 조작된 페니실린 G 아실라제를 제조하는 방법을 제공한다. 일부 실시양태에서, 본 방법은 제조된 조작된 페니실린 G 아실라제를 회수하는 단계를 추가로 포함한다.

본 발명은 또한 i) 본원에 제공된 적어도 하나의 조작된 페니실린 G 아실라제 및/또는 본원에 제공된 적어도 하나의 조작된 페니실린 G 아실라제를 포함하는 조성물, 및 A1/B1/B29 트리-페닐 아세테이트 보호기를 포함하는 인슐린 또는 유리 인슐린을 제공하는 단계; 및 ii) 조작된 페니실린 G 아실라제가 A1/B1/B29 트리-페닐 아세테이트 보호기를 제거하고, 유리 인슐린, 이중 보호된 인슐린 또는 단일 보호된 인슐린이 생산되는 조건하에서 조작된 페니실린 G 아실라제를 A1/B1/B29 트리-페닐 아세테이트 보호기를 포함하는 인슐린에 노출시키는 단계; iii) 조작된 페니실린 G 아실라제가 페닐 아세테이트 보호기를 부가하고, 삼중 보호된 인슐린, 이중 보호된 인슐린 또는 단일 보호된 인슐린이 생산되는 조건하에서 조작된 페니실린 G 아실라제를 인슐린에 노출시키는 단계를 포함하는, 페닐 아세테이트 보호된 인슐린을 제조하는 방법을 제공한다. 본 발명의 일부 실시양태에서, 페니실린 G 아실라제는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288과 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99% 또는 그 이상 동일하다. 본 발명의 일부 실시양태에서, 페니실린 G 아실라제는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288과 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하다. 본 발명의 일부 추가의 실시양태에서, 페니실린 G 아실라제는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288을 포함한다. 일부 실시양태에서, 페니실린 G 아실라제는 서열 번호: 4-1902에 제공된 적어도 하나의 짝수 번호의 서열과 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하다. 일부 실시양태에서, 조작된 페니실린 G 아실라제는 야생형 페니실린 G 아실라제보다 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 초과 또는 그 이상으로 유리 인슐린을 생산한다. 본 발명은 또한 본 발명의 방법을 사용하여 제조된 유리 인슐린을 포함하는 조성물을 제공한다. 일부 실시양태에서, 본 발명은 본원에 제공된 임의의 방법에 따라 제조된 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 포함하는 조성물을 제공한다.

본 발명은 또한 i) 본원에 제공된, 적어도 하나의 조작된 페니실린 G 아실라제 및/또는 적어도 하나의 조작된 페니실린 G 아실라제를 포함하는 조성물, 및 유리 인슐린을 제공하는 단계; 및 ii) 조작된 페니실린 G 아실라제가 A1, B1, 및/또는 B29 위치를 아실화시키는 조건하에서 조작된 페니실린 G 아실라제를 인슐린에 노출시킴으로써 단일 보호된 또는 이중 보호된 인슐린을 제조하는 단계를 포함하는, 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 제조하는 방법을 제공한다. 일부 실시양태에서, 페니실린 G 아실라제는 인슐린의 A1 위치를 아실화시키는 반면, 일부 다른 실시양태에서, 페니실린 G 아실라제는 인슐린의 B1 위치를 아실화시키고 또 다른 추가의 실시양태에서, 페니실린 G 아실라제는 인슐린의 B29 위치를 아실화시킨다. 일부 실시양태에서, 페니실린 G 아실라제는 인슐린의 A1, B1, 및 B29 위치를 아실화시킨다. 일부 추가의 실시양태에서, 조작된 페니실린 G 아실라제는, 서열 번호: 2의 폴리펩티드에 의한 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린의 생산과 비교하였을 때, 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 초과 또는 그 이상으로 생산한다. 일부 추가의 실시양태에서, A1, B1 및/또는 B29를 아실화시키는 데 사용되는 페니실린 G 아실라제는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상의 동일성을 가지는 서열을 포함한다. 일부 실시양태에서, 페니실린 G 아실라제는 서열 번호: 4-1902에 제공된 적어도 하나의 짝수 번호의 서열과 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하다.

본 방법의 일부 추가의 실시양태에서, 페니실린 G 아실라제는 표 5.1, 6.1, 7.1, 8.1, 8.2, 9,1, 10.1, 12.1, 12.2, 12.3, 12.4, 12.5, 12.6, 및/또는 12.7 중의 적어도 하나의 서열과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상의 동일성을 가지는 서열을 포함한다. 본 방법의 또 다른 추가의 실시양태에서, 페니실린 G 아실라제는 표 5.1, 6.1, 7.1, 8.1, 8.2, 9,1, 10.1, 12.1, 12.2, 12.3, 12.4, 12.5, 12.6, 및/또는 12.7 중 임의의 것에 기재된 서열을 포함한다.

본 발명은 또한 i) 제1항의 조작된 페니실린 G 아실라제, 및 인슐린을 제공하는 단계; 및 ii) 조작된 페니실린 G 아실라제가 A1, B1 및/또는 B29 트리-페닐 아세테이트 보호기를 인슐린에 부가하는 조건하에서 조작된 페니실린 G 아실라제를 인슐린에 노출시킴으로써 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 제조하는 단계를 포함하는, 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 제조하는 방법을 제공한다. 일부 실시양태에서, 페니실린 G 아실라제는 인슐린의 A1 트리-페닐 아세테이트 보호기를 부가하는 반면, 일부 추가의 실시양태에서, 페니실린 G 아실라제는 인슐린의 B1 트리-페닐 아세테이트 보호기를 부가하고, 또 다른 추가의 실시양태에서, 페니실린 G 아실라제는 인슐린의 B29 트리-페닐 아세테이트 보호기를 부가한다. 일부 추가의 실시양태에서, 페니실린 G 아실라제는 인슐린의 A1, B1, 및 B29 트리-페닐 아세테이트 보호기를 부가한다. 일부 실시양태에서, 조작된 페니실린 G 아실라제는, 서열 번호: 2의 폴리펩티드에 의한 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린의 생산과 비교하였을 때, 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 초과 또는 그 이상으로 생산한다. 일부 추가의 실시양태에서, 페니실린 G 아실라제는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상의 동일성을 가지는 서열을 포함한다. 일부 실시양태에서, 페니실린 G 아실라제는 서열 번호: 4-1902에 제공된 적어도 하나의 짝수 번호의 서열과 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하다.

본 발명은 또한 본원에 제공된 방법 중 임의의 것에 따라 제조된 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 포함하는 조성물을 제공한다.

본 발명은, 페니실린 G 아실라제가 서열 번호: 837, 897, 1219, 또는 1859를 포함하는 방법을 추가로 제공한다.

도면의 간단한 설명
도 1은 인슐린을 정량화하는 데 사용된 분석 방법의 크로마토그램, 및 아실화된 생성물의 용출 순서를 제공한다.
도 2a 내지 도 2h는 실시예 11에 기술된 실험 결과를 제공한다.

본 발명의 설명

본 발명은 매우 다양한 β 락탐 항생제의 합성에서 중요한 중간체인, 페닐 아세트산 및 6-아미노페니실란산(6-APA)으로 페니실린을 절단할 수 있는 조작된 페니실린 G 아실라제(PGA)를 제공한다. 특히, 본 발명은 보호기를 유리 인슐린의 A1, B1 또는 B29 위치에 부가함으로써, 또는 A1/B1/B29 삼중 보호된 인슐린으로부터 보호기를 제거하거나, 또는 A1/B1/B29 트리-페닐 아세테이트 보호기를 제거함으로써 유리 인슐린을 방출할 수 있는 조작된 PGA를 제공한다.

일반적으로, 자연적으로 발생된 PGA는 알파 서브유니트 및 베타 서브유니트로 구성된 이종이량체 효소이다. 야생형 PGA는 주변 세포질로의 이동을 매개하는 N 말단 신호 펩티드 및 알파 서브유니트의 C 말단을 베타 서브유니트의 N 말단에 연결시키는 링커 영역을 함유하는, 프리-프로-PGA 폴리펩티드로서 천연적으로 합성된다. 단백질가수분해 프로세싱은 성숙한 이종이량체 효소를 유도한다. 분자간 링커 영역은 또한 효소의 적절한 폴딩을 촉진시키는 작용을 할 수 있다. 본원에 제공된 PGA는 하기에 상세하게 기술되는 바와 같은 개선된 효소 특성을 생성하기 위해 다양한 변형이 도입된, 클루이베라 시트로필라(Kluyvera citrophila) 유래의 PGA를 기반으로 한다.

본원에 제공된 설명에서, 단수의 사용은 달리 구체적으로 언급되지 않는 한, 복수의 것을 포함한다(그리고 그 반대의 경우도 그러하다). 예를 들어, 단수 형태인 "하나"("a," "an") 및 "그"는 문맥상 달리 명백하게 명시되지 않는 한, 복수의 지시 대상을 포함한다. 유사하게, "포함하다(comprise)," "포함하다(comprises)," "포함하는(comprising)," "포함하다(include)," "포함하다(includes)", 및 "포함하는(including)"은 상호교환적일 수 있고, 제한하고자 하는 것이 아니다. 다양한 실시양태의 설명에서 "포함하는"이라는 용어가 사용되는 경우, 당업자는 일부 구체적인 경우에서, 한 실시양태는 "~으로 실질적으로 구성된" 또는 "~으로 구성된"이라는 표현을 사용하여 대안적으로 기술될 수 있다는 것을 이해하게 된다는 것을 추가로 이해하여야 한다.

도면을 비롯한, 상기의 일반 설명, 및 하기의 상세한 설명, 이 둘 모두는 오직 예시적이고, 설명적인 것이며, 본 개시내용을 제한하지 않는다. 더욱이, 본원에서 사용된 섹션 표제는 오직 조직화를 위한 것이며, 기술된 주제를 제한하는 것으로 해석되지 않아야 한다.

정의

본원에서 사용되는 바, 하기 용어는 하기 의미를 가지는 것으로 의도된다.

본 개시내용과 관련하여, 본원의 설명에서 사용되는 기술 및 과학 용어는 구체적으로 달리 정의되지 않는 한, 당업자가 보편적으로 이해하는 의미를 가질 것이다. 따라서, 하기 용어들은 하기 의미를 가지는 것으로 의도된다. 본원에 인용된, 모든 특허 및 공개문헌은, 상기 특허 및 공개문헌 내에 개시된 모든 서열을 포함하여 명백하게 참조로 포함된다. 달리 명시되지 않는 한, 본 발명의 실시는 당업자에게 알려진, 분자 생물학, 발효, 미생물학, 및 관련 분야에서 보편적으로 사용되는 통상의 기술을 포함한다. 본원에서 달리 정의되지 않는 한, 본원에서 사용되는 모든 기술 및 과학 용어는 본 발명이 속하는 분야의 숙련가가 보편적으로 이해하는 것과 동일한 의미를 가진다. 본원에 기술된 것과 유사하거나 또는 등가인 임의의 방법 및 물질이 본 발명의 실시 또는 시험에서 사용될 수 있지만, 바람직한 방법 및 물질이 기술된다. 실제로, 본 발명은 방법, 프로토콜, 및 시약이 사용되는 맥락에 따라 달라질 수 있는 바, 본원에 기술된 특정 방법, 프로토콜, 및 시약으로 제한되지 않는 것으로 의도된다. 본원에 제공된 표제는 본 발명의 다양한 측면 또는 실시양태의 제한이 아니다.

그럼에도 불구하고, 본 발명의 이해를 촉진시키기 위해, 다수의 용어가 하기에서 정의된다. 수치 범위는 그 범위를 한정하는 수치를 포함한다. 따라서, 본원에 개시된 모든 수치 범위는, 더 좁은 수치 범위가 모두 본원에 명백하게 기재된 것처럼, 더 넓은 수치 범위에 속하는 상기 모든 좁은 수치 범위를 포함하는 것으로 의도된다. 또한, 본원에 개시된 모든 최대(또는 최소) 한계는 더 낮은(또는 더 높은) 수치 한계가 본원에 명백하게 기재된 것처럼, 상기 모든 더 낮은(또는 더 높은) 수치 한계를 포함하는 것으로 의도된다.

본원에서 사용되는 바, "포함하는(comprising)"이라는 용어 및 그의 동족어는 그의 포괄적인 의미(즉, "포함하는(including)"이라는 용어 및 그의 동족어와 등가)로 사용된다.

본원 및 첨부된 특허청구범위에서 사용되는 바, 단수 "하나"("a," "an") 및 "그"는 문맥상 달리 명백하게 명시되지 않는 한, 복수의 지시 대상을 포함한다. 따라서, 예를 들어, 한 "숙주 세포"에 대한 언급은 복수 개의 상기 숙주 세포를 포함한다.

달리 명시되지 않는 한, 각각, 핵산은 좌측에서 우측으로 5'에서 3' 방향으로 및 아미노산 서열은 좌측에서 우측으로 아미노에서 카복시 방향으로 기재된다.

본원에 제공된 표제는 전체로서 본 명세서에 참조될 수 있는 본 발명의 다양한 측면 또는 실시양태의 제한이 아니다. 따라서, 하기 정의되는 용어들은 전체로 본 명세서를 참조하여 더욱 상세하게 정의된다.

본원에서 사용되는 바, "단백질," "폴리펩티드," 및 "펩티드"라는 용어는 본원에서 상호교환적으로 사용되고, 이는 길이 또는 번역후 변형(예컨대, 글리코실화, 인산화, 지질화, 미리스틸화, 유비퀴틴화 등)과 상관없이, 아미드 결합에 의해 공유적으로 연결된 적어도 2개의 아미노산으로 이루어진 중합체를 의미한다. D-아미노산 및 L-아미노산, 및 D-아미노산과 L-아미노산의 혼합물이 본 정의에 포함된다.

본원에서 사용되는 바, "폴리뉴클레오티드" 및 "핵산'은 함께 공유적으로 연결된 2개 이상의 뉴클레오시드를 지칭한다. 폴리뉴클레오티드는 전체로 리보뉴클레오시드(즉, RNA)로 구성되거나, 전체로 2' 데옥시리보뉴클레오티드(즉, DNA) 또는 리보뉴클레오시드 및 2' 데옥시리보뉴클레오시드의 혼합물로 구성될 수 있다. 뉴클레오시드는 전형적으로 표준 포스포디에스테르 연결을 통해 함께 연결되지만, 폴리뉴클레오티드는 하나 이상의 비표준 연결을 포함할 수 있다. 폴리뉴클레오티드는 단일 가닥, 또는 이중 가닥일 수 있거나, 또는 단일 가닥 영역 및 이중 가닥 영역, 둘 모두를 포함할 수 있다. 더욱이, 폴리뉴클레오티드가 전형적으로 자연적으로 발생된 코딩 핵염기(즉, 아데닌, 구아닌, 우라실, 티민, 및 시토신)로 구성되는 반면, 하나 이상의 변형된 핵염기 및/또는 합성 핵염기(예컨대, 이노신, 크산틴, 히포크산틴 등)를 포함할 수 있다. 바람직하게, 상기 변형된 핵염기 또는 합성 핵염기는 코딩 핵염기가 될 것이다.

본원에서 사용되는 바, "하이브리드화 엄격성"은 핵산의 하이브리드화시, 세척 조건과 같은, 하이브리드화 조건에 관한 것이다. 일반적으로, 하이브리드화 반응은 더 낮은 엄격성 조건하에서 수행된 후, 다양하지만, 더 높은 엄격성의 세척이 진행된다. "중간 정도로 엄격한 하이브리드화"라는 용어는 표적 DNA가, 표적 DNA와 약 60%의 동일성, 바람직하게, 약 75%의 동일성, 약 85%의 동일성을 가지는; 표적 폴리뉴클레오티드와 약 90% 초과의 동일성을 가지는 상보적인 핵산에 결합할 수 있도록 허용하는 조건을 지칭한다. 예시적인 중간 정도로 엄격한 조건은 42℃에서 50% 포름아미드, 5Х 덴하르트 용액(Denhart's solution), 5ХSSPE, 0.2% SDS에서 하이브리드화 후, 42℃에서 0.2ХSSPE, 0.2% SDS에서 세척하는 것과 등가인 조건이다. "고엄격성 하이브리드화"는 일반적으로 정의진 폴리뉴클레오티드 서열에 대한 용해 조건하에서 측정된 바와 같은 열적 용융 온도 T _m 으로부터 약 10℃ 이하인 조건을 의미한다. 일부 실시양태에서, 고엄격성 조건은 65℃에서 0.018M NaCl 중에서 안정한 하이브리드를 형성하는 오직 그들 핵산 서열만의 하이브리드화를 가능하게 하는 조건을 지칭한다(즉, 만약 하이브리드가 65℃에서 0.018M NaCl에서 안정적이지 않다면, 본원에서 고려하는 바와 같이, 고엄격성 조건하에서 안정하지 않을 것이다). 고엄격성 조건은 예를 들어, 42℃에서 50% 포름아미드, 5Х 덴하르트 용액, 5ХSSPE, 0.2% SDS와 등가인 조건에서 하이브리드화한 후, 이어서, 65℃에서 0.1ХSSPE, 및 0.1% SDS에서 세척하는 것으로 제공될 수 있다. 또 다른 고엄격성 조건은 65℃에서 0.1%(w:v) SDS를 함유하는 5X SSC에서 하이브리드화하고, 65℃에서 0.1% SDS를 함유하는 0.1x SSC에서 세척하는 것과 등가인 조건에서 하이브리드화하는 것이다. 다른 고엄격성 하이브리드화 조건 뿐만 아니라, 중간 정도로 엄격한 조건도 당업자에게 공지되어 있다.

본원에서 사용되는 바, "코딩 서열"은 단백질의 아미노산 서열을 코딩하는 핵산(예컨대, 유전자)의 일부를 지칭한다.

본원에서 사용되는 바, "코돈 최적화"는 코딩된 단백질이 관심 유기체에서 효율적으로 발현되도록 하기 위한, 단백질을 코딩하는 폴리뉴클레오티드의 코돈을 특정 유기체에서 우선적으로 사용되는 것으로 변화시키는 것을 지칭한다. 일부 실시양태에서, PGA 효소를 코딩하는 폴리뉴클레오티드는 발현을 위해 선택된 숙주 유기체로부터 최적 생산을 위해 코돈 최적화될 수 있다. 비록 유전자 코돈은 대부분의 아미노산이 "동의성" 또는 "동의" 코돈이라고 불리는, 몇몇 코돈으로 대표되는 축퇴성이기는 하지만, 특정 유기체에 의한 코돈 용법은 특정 코돈 트리플렛 쪽으로의 비무작위적이고, 편향적인 것으로 널리 공지되어 있다. 이러한 코돈 용법 편향성은 주어진 유전자, 공통 기능 또는 조상 기원의 유전자들, 저카피수 단백질 대비 고발현 단백질, 및 유기체 게놈의 집합 단백질 코딩 영역과 관련하여 더 높을 수 있다. 일부 실시양태에서, PGA 효소를 코딩하는 폴리뉴클레오티드는 발현을 위해 선택된 숙주 유기체로부터 최적 생성을 위해 코돈 최적화될 수 있다.

본원에서 사용되는 바, "바람직한, 최적의, 높은 코돈 용법 편향 코돈"은 상호교환적으로 동일한 아미노산을 코딩하는 다른 코돈보다 단백질 코딩 영역에서 더 높은 빈도로 사용되는 코돈을 지칭한다. 바람직한 코돈은 단일 유전자의 코돈 용법, 공통 기능 또는 기원의 유전자 세트, 고도로 발현되는 유전자, 전체 유기체의 집합 단백질 코딩 영역의 코돈 빈도, 관련 유기체의 집합 단백질 코딩 영역의 코돈 빈도, 또는 그의 조합과 관련하여 결정될 수 있다. 그 빈도가 유전자 발현 수주에 따라 증가하는 코돈이 전형적으로 발현에 최적인 코돈이다. 예를 들어, 군집 분석 또는 대응 분석을 사용하는, 다변량 분석, 및 유전자에서 사용되는 코돈의 유효수를 비롯한, 특정 유기체에서 코돈 빈도(예컨대, 코돈 용법, 상대적 동의 코돈 용법) 및 코돈 선호도를 결정하는 다양한 방법이 공지되어 있다(예컨대, 문헌 [GCG CodonPreference, Genetics Computer Group Wisconsin Package; CodonW, John Peden, University of Nottingham; McInerney, Bioinform., 14:372-73 [1998]]; [Stenico et al., Nucleic Acids Res., 222:437-46 [1994]; 및 [Wright, Gene 87:23-29 [1990]] 참조). 유기체 목록 증가를 위해 코돈 용법 표가 이용가능하다(예컨대, 문헌 [Wada et al., Nucleic Acids Res., 20:2111-2118 [1992]]; [Nakamura et al., Nucl. Acids Res., 28:292 [2000]]; [Duret, et al., 상기 문헌 동일]; [Henaut and Danchin, "Escherichia coli and Salmonella," Neidhardt, et al. (eds.), ASM Press, Washington D.C., [1996], p. 2047-2066] 참조). 코돈 용법을 획득하기 위한 데이터 공급원은 단백질을 코딩할 수 있는 임의의 이용가능한 뉴클레오티드 서열에 의존할 수 있다. 이들 데이터 세트는 발현되는 단백질을 코딩하는 것으로 실제로 알려진 핵산 서열(예컨대, 완전한 단백질 코딩 서열-CDS(complete protein coding sequence)), 발현된 서열 태그(ESTS: expressed sequence tags), 또는 게놈 서열의 예상되는 코딩 영역을 포함한다(예컨대, 문헌 [Uberbacher, Meth. Enzymol., 266:259-281 [1996]]; [Tiwari et al., Comput. Appl. Biosci., 13:263-270 [1997]] 참조).

본원에서 사용되는 바, "제어 서열"은 본원에서 본 발명의 폴리뉴클레오티드 및/또는 폴리펩티드의 발현에 필요하거나, 또는 이로운, 모든 성분을 포함하는 것으로 정의된다. 각각의 제어 서열은 관심 폴리뉴클레오티드에 천연적이거나, 또는 외래의 것일 수 있다. 그러한 제어 서열로는 리더, 폴리아데닐화 서열, 프로펩티드 서열, 프로모터, 신호 펩티드 서열, 및 전사 종결인자를 포함하나, 이에 제한되지 않는다.

본원에서 사용되는 바, "작동가능하게 연결된"은 본원에서 제어 서열이 관심 폴리뉴클레오티드 및/또는 폴리펩티드의 발현을 지시하거나, 또는 조절할 수 있도록 제어 서열이 관심 폴리뉴클레오티드에 대한 위치에 적절하게 배치(기능적 관계로)되어 있는 배열로서 정의된다.

본원에서 사용되는 바, "프로모터 서열"은 예컨대, 코딩 서열과 같은, 관심 폴리뉴클레오티드의 발현을 위해 숙주 세포에 의해 인식되는 핵산 서열을 지칭한다. 제어 서열은 적절한 프로모터 서열을 포함할 수 있다. 프로모터 서열은 관심 폴리뉴클레오티드의 발현을 매개하는 전사 제어 서열을 함유한다. 프로모터는 돌연변이체, 말단절단형, 및 하이브리드 프로모터를 비롯한, 선택된 숙주 세포 내에서 전사 활성을 보이는 임의의 핵산 서열일 수 있고, 숙주 세포에 상동성이거나 또는 이종성인 세포외 또는 세포내 폴리펩티드를 코딩하는 유전자로부터 수득될 수 있다.

본원에서 사용되는 바, "자연적으로 발생된" 또는 "야생형"은 자연상에서 발견되는 형태를 지칭한다. 예를 들어, 자연적으로 발생된 또는 야생형 폴리펩티드 또는 폴리뉴클레오티드 서열은 자연상의 공급원으로부터 단리될 수 있고, 인간 조작에 의해 의도적으로 변형되지 않은 유기체에 존재하는 서열이다.

본원에서 사용되는 바, (예컨대, 세포, 핵산, 또는 폴리펩티드)와 관련하여, 본 개시내용에서 사용될 때, "비자연적으로 발생된," "조작된," 및 "재조합"은 달리 자연상에 존재하지 않는 방식으로 변형된, 물질, 또는 상기 물질의 자연 또는 천연 형태에 상응하는 물질을 지칭한다. 일부 실시양태에서, 물질은 자연적으로 발생된 물질과 동일하지만, 합성 물질로부터 및/또는 재조합 기술을 사용하는 조작에 의해 제조 또는 유도된 것이다. 비제한적인 예로는 다른 것들 중에서도 특히 세포의 천연(비재조합) 형태에서는 발견되지 않거나, 또는 그렇지 않으면 다른 수준으로 발현되는 천연 유전자를 발현하는 유전자를 발현하는 재조합 세포를 포함한다.

본원에서 사용되는 바, "서열 동일성(%)" "동일성(%)" 및 "% 동일"은 폴리뉴클레오티드 서열 사이 및 폴리펩티드 서열 사이의 비교를 지칭하고, 비교창에 걸쳐 최적으로 정렬된 두 서열을 비교함으로써 측정되고, 여기서, 상기 비교창의 폴리뉴클레오티드 또는 폴리펩티드 서열의 일부분은 두 서열의 최적의 정렬을 위해, 참조 서열과 비교하여 부가 또는 결실(즉, 갭)을 포함할 수 있다. 백분율(%)은 동일한 핵산 염기 또는 아미노산 잔기가 두 서열 모두에 존재하는 위치, 또는 핵산 염기 또는 아미노산 잔기가 갭과 함께 정렬된 위치의 개수를 측정하여 매칭되는 위치의 개수를 산출하고, 매칭되는 위치의 개수를 비교창 중 위치의 총 개수로 나누고, 그 결과에 100을 곱하여 서열 동일성(%)을 산출함으로써 계산된다. 최적의 정렬 및 서열 동일성(%) 측정은 BLAST 및 BLAST 20 알고리즘을 사용하여 수행된다(예컨대, 문헌 [Altschul et al., J. Mol. Biol. 215: 403-410 [1990]]; 및 [Altschul et al., Nucl. Acids Res. 3389-3402 [1977]] 참조). BLAST 분석을 수행하기 위한 소프트웨어는 미국 국립 생명공학 정보 센터(National Center for Biotechnology Information)의 웹사이트를 통해 공개적으로 이용가능하다.

간략하면, BLAST 분석은 먼저, 데이터베이스 서열 중에서 길이가 동일한 워드와 정렬되었을 때, 일부 양성 값 역치 점수(T)와 매칭되거나, 또는 그를 만족하는 질의 서열 중의 짧은 워드 길이(W)를 확인함으로써 높은 점수의 서열 쌍(HSP: high scoring sequence pair)을 확인하는 것을 포함한다. T는 이웃 워드 점수 역치로서 지칭된다(문헌 [Altschul et al, 상기 문헌 동일]. 이들 초기 이웃 워드 히트는 그들을 함유하는 더 긴 HSP를 찾도록 검색을 개시하기 위한 씨드로서 작용한다. 이어서, 히트를 누적 정렬 점수가 증가될 수 있는 만큼 멀리 각 서열을 따라 양쪽 방향으로 연장시킨다. 누적 점수는 뉴클레오티드 서열의 경우, 파라미터 M(매칭되는 잔기 쌍에 대한 보상 점수; 항상 > 0임) 및 N(미스매칭되는 잔기에 대한 패널티 점수; 항상 < 0임)을 사용하여 계산된다. 아미노산 서열의 경우, 점수화 매트릭스가 누적 점수를 계산하는 데 사용된다. 각 방향으로 워드 히트의 연장은 누적 정렬 점수가 그의 최대 획득 값으로부터 정량 X만큼 하락하였을 때; 누적 점수가 하나 이상의 음수 잔기 정렬의 축적으로 인해 0 이하가 되거나; 또는 양쪽 서열의 끝에 도달하였을 때 중지된다. BLAST 알고리즘 파라미터 W, T, 및 X는 정렬의 감도 및 속도를 결정한다. BLASTN 프로그램(뉴클레오티드 서열인 경우)은 디폴트로서 워드길이(W) = 11, 기대치(E) = 10, M=5, N=-4, 및 양쪽 가닥의 비교를 사용한다. 아미노산 서열인 경우, BLASTP 프로그램은 디폴트로서 워드길이(W) = 3, 기대치(E) = 10, 및 BLOSUM62 점수화 매트릭스를 사용한다(예컨대, 문헌 [Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915 [1989]] 참조).

2개 서열에 대한 동일성(%)을 제공하는 데 BLAST와 유사하게 작용하는 다수의 다른 알고리즘이 당업계에서 이용가능하며, 공지되어 있다. 비교를 위한 서열의 최적의 정렬은 당업계에 공지된 임의의 적합한 방법을 사용하여 (예컨데, 문헌 [Smith and Waterman, Adv. Appl. Math. 2:482 [1981]]의 국소 상동성 알고리즘에 의해; [Needleman and Wunsch, J. Mol. Biol. 48:443 [1970]]의 상동성 정렬 알고리즘에 의해; [Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444 [1988]]의 유사성 검색 방법에 의해; 및/또는 상기 알고리즘([GCG 위스콘신 소프트웨어 패키지의 GAP, BESTFIT, FASTA, 및 TFASTA]의 컴퓨터 실행에 의해), 또는 당업계에 일반적으로 알려져 있는 방법을 사용하여 시각적 검사에 의해 수행될 수 있다. 추가적으로, 서열 정렬 및 서열 동일성(%) 측정은 제공된 디폴트 파라미터를 사용하여 GCG 위스콘신 소프트웨어 패키지의 BESTFIT 또는 GAP 프로그램(Accelrys, 미국 위스콘신주 매디슨 소재)을 사용할 수 있다.

본원에서 사용되는 바, "실질적 동일성"은 적어도 20개의 잔기 위치로 이루어진 비교창, 빈번하게는 적어도 30-50개의 잔기로 이루어진 창에 걸쳐 참조 서열과 비교하였을 때, 적어도 80%의 서열 동일성, 적어도 85%의 서열 동일성 및 89 내지 95%의 서열 동일성, 더욱 일반적으로는 적어도 99%의 서열 동일성을 가지는 폴리뉴클레오티드 또는 폴리펩티드 서열을 지칭하고, 서열 동일성(%)은 비교창에 걸쳐 참조 서열의 총 20% 이하인 결실 또는 부가를 포함하는 서열과 참조 서열을 비교함으로써 계산된다. 폴리펩티드에 적용되는 구체적인 실시양태에서, "실질적 동일성"이란, 두 폴리펩티드가 최적으로 정렬되었을 때, 예컨대, 디폴트 갭 가중치를 사용하는 프로그램 GAP 또는 BESTFIT에 의해 적어도 80%의 서열 동일성, 바람직하게, 적어도 89%의 서열 동일성, 적어도 95%의 서열 동일성 또는 그 이상(예컨대, 99%의 서열 동일성)을 공유하는 것을 지칭한다. 일부 바람직한 실시양태에서, 동일하지 않은 잔기 위치는 보존적 아미노산 치환이 다르다.

본원에서 사용되는 바, "참조 서열"은 또 다른 서열의 비교 대상이 되는 정의된 서열을 지칭한다. 참조 서열은 더 큰 서열의 서브세트, 예를 들어, 전장의 유전자 또는 폴리펩티드 서열의 세그먼트일 수 있다. 일반적으로, 참조 서열은 길이가 적어도 20개의 뉴클레오티드 또는 아미노산 잔기 길이, 적어도 25개의 잔기 길이, 적어도 50개의 잔기 길이, 또는 핵산 또는 폴리펩티드의 전장인 길이이다. 두 폴리뉴클레오티드 또는 폴리펩티드가 각각 (1) 두 서열 간에 유사한 서열(즉, 완전한 서열의 일부)을 포함하고, (2) 두 서열 간에 다른 서열을 추가로 포함할 수 있으므로, 2개(또는 그 이상의) 폴리뉴클레오티드 또는 폴리펩티드 간의 서열 비교는 전형적으로 비교창에 걸쳐 두 폴리뉴클레오티드의 서열을 비교함으로써 서열 유사성의 국소 영역을 확인하고, 비교함으로써 수행된다. "참조 서열"이라는 용어는 야생형 서열로 제한되는 것으로 의도되지 않고, 조작되거나, 또는 변경된 서열을 포함할 수 있다. 예를 들어, 일부 실시양태에서, "참조 서열"은 사전에 조작되거나, 또는 변경된 아미노산 서열일 수 있다.

본원에서 사용되는 바, "비교창"은 적어도 약 20개의 인접한 뉴클레오티드 위치 또는 아미노산 잔기의 개념적 절편을 지칭하고, 여기서, 서열은 적어도 20개의 인접한 뉴클레오티드 또는 아미노산의 참조 서열과 비교될 수 있고, 비교창 내 서열의 일부분은 두 서열의 최적의 정렬을 위해 (부가 또는 결실을 포함하지 않는) 참조 서열과 비교하였을 때, 20% 이하의 부가 또는 결실(즉, 갭)을 포함할 수 있다. 비교창은 20개보다 긴 인접한 잔기일 수 있고, 임의적으로, 30, 40, 50, 100개, 또는 그보다 긴 창을 포함한다.

본원에서 사용되는 바, 주어진 아미노산 또는 폴리뉴클레오티드 서열의 넘버링과 관련하여 사용할 때, "∼에 상응하는," "∼와 관련하여," 및 "∼ 대비"는 주어진 아미노산 또는 폴리뉴클레오티드 서열을 참조 서열과 비교하는 경우의 언급된 참조 서열의 잔기의 넘버링을 지칭한다. 다시 말해, 주어진 중합체의 잔기 번호 또는 잔기 위치는 주어진 아미노산 또는 폴리뉴클레오티드 서열 내 잔기의 실제 번호 위치보다는 참조 서열에 대해 지정된다. 예를 들어, 주어진 아미노산 서열, 예컨대, 조작된 PGA의 아미노산 서열은 두 서열 사이의 잔기 매치를 최적화하기 위해 갭을 도입시킴으로써 참조 서열에 대해 정렬시킬 수 있다. 이러한 경우, 비록 갭이 존재하여도, 주어진 아미노산 또는 폴리뉴클레오티드 서열 내 잔기의 넘버링은 정렬시킨 참조 서열에 대해 이루어진다. 본원에서 사용되는 바, 하기에서 추가로 기술되는 바와 같이, 잔기 위치, 예컨대, "Xn"에 대한 언급은 달리 구체적으로 언급되지 않는 한, "∼에 상응하는 잔기"를 지칭하는 것으로 이해되어야 한다. 따라서, 예를 들어, "X94"는 폴리펩티드 서열에서 94번 위치의 임의의 아미노산을 지칭한다.

본원에서 사용되는 바, "개선된 효소 특성"은 참조 PGA와 비교하였을 때, 임의의 효소 특성의 개선을 나타내는 PGA를 지칭한다. 비록 일부 실시양태에서, 참조 PGA는 또 다른 개선된 조작된 PGA일 수 있지만, 본원에 기술된 조작된 PGA 폴리펩티드의 경우, 비교는 일반적으로 야생형 PGA 효소에 대해 이루어진다. 개선이 바람직할 수 있는 효소 특성으로는 효소 활성(명시된 양의 PGA를 사용한 명시된 반응 시간에서의 기질의 전환율(%)로 표시될 수 있다), 화학선택성, 열 안정성, 용매 안정성, pH 활성 프로파일, 보조인자 요구, 억제제에 대한 무반응성(예컨대, 생성물 억제), 입체특이성 및 입체선택성(거울상 선택성 포함)을 포함하나, 이에 제한되지 않는다.

본원에서 사용되는 바, "증가된 효소 활성"은 참조 PGA 효소와 비교하여 비활성(예컨대, 제조된 생성물/시간/단백질 중량)의 증가 또는 기질의 생성물로의 전환율(%)(예컨대, 명시된 양의 PGA를 사용한 명시된 기간 동안 출발량의 기질의 생성물로의 전환율(%))의 증가로 표현될 수 있는, 조작된 PGA 폴리펩티드의 개선된 특성을 지칭한다. 효소 활성을 측정하는 예시적인 방법은 실시예에 제공되어 있다. 그의 변화가 효소 활성을 증가시킬 수 있는 것인 K _m , V _최대 또는 k _cat 인 고전적인 효소 특성을 비롯한, 효소 활성과 관련된 임의의 특성이 영향을 받을 수 있다. 효소 활성의 개선은 상응하는 야생형 PGA 효소의 효소 활성의 약 1.5배부터, 자연적으로 발생된 PGA, 또는 PGA 폴리펩티드 유래 기점이 된 또 다른 조작된 PGA보다 2배, 5배, 10배, 20배, 25배, 50배, 75배, 100배 이상만큼의 효소 활성일 수 있다. 구체적인 실시양태에서, 조작된 PGA 효소는 모체 PGA 효소보다 1.5 내지 50배, 1.5 내지 100배 범위로 더 큰 개선된 효소 활성을 나타낸다. 임의의 효소의 활성은 확산 제한적인 바, 이에 촉매 전환 속도는 임의의 필요한 보조인자를 비롯한, 기질의 확산 속도를 초과할 수 없다는 것을 당업자는 이해한다. 확산 한계, 또는 k _cat /K _m 의 이론상 최대값은 일반적으로 약 10⁸ 내지 10⁹ (M^-1 s^{- 1})이다. 따라서, PGA의 효소 활성의 임의의 개선은 PGA 효소에 의해 작용을 받는 기질의 확산 속도와 관련된 상한계를 가지게 된다. PGA 활성은 페니실린 G의 절단시 페닐아세트산의 방출을 측정하기 위해 사용되는 표준 검정법 중 어느 하나, 예컨대, 적정법에 의해 측정될 수 있다(예컨대, 문헌 [Simons and Gibson, Biotechnol. Tech.,13:365-367 [1999]] 참조) . 일부 실시양태에서, PGA 활성은 절단 생성물 5-아미노-2-니트로-벤조산을 분광광도법으로 검출가능한(λ최대 = 405 nm), 6-니트로페닐아세트아미도 벤조산(NIPAB)을 사용하여 측정될 수 있다. 효소 활성의 비교는 본원에 상세하게 추가로 기술되는 바와 같이, 정의된 효소 제제, 설정된 조건하에서의 정의된 검정법, 및 하나 이상의 정의된 기질을 사용하여 이루어진다. 일반적으로, 용해물을 비교하는 경우, 숙주 세포에 의해 생산되고, 용해물에 존재하는 효소의 양의 변동을 최소화하기 위해 동일한 발현 시스템 및 동일한 숙주 세포를 사용하는 것 뿐만 아니라, 세포의 개수 및 검정되는 단백질의 양을 측정한다.

본원에서 사용되는 바, "증가된 효소 활성" 및 "증가된 활성"은 본원에 기술된 바와 같이 참조 효소와 비교하여 비활성(예컨대, 제조된 생성물/시간/단백질 중량)의 증가 또는 기질의 생성물로의 전환율(%)(예컨대, 명시된 양의 PGA를 사용한 명시된 기간 동안 출발량의 기질의 생성물로의 전환율(%))의 증가로 표현될 수 있는, 조작된 효소의 개선된 특성을 지칭한다. 그의 변화가 효소 활성을 증가시킬 수 있는 것인 K _m , V _최대 또는 k _cat 인 고전적인 효소 특성을 비롯한, 효소 활성과 관련된 임의의 특성이 영향을 받을 수 있다. 일부 실시양태에서, 본원에 제공된 PGA 효소는 인슐린의 특정 잔기로부터 트리-페닐 아세테이트 보호기를 제거하여 인슐린을 유리시킨다. 효소 활성의 비교는 본원에 상세하게 추가로 기술되는 바와 같이, 정의된 효소 제제, 설정된 조건하에서의 정의된 검정법, 및 하나 이상의 정의된 기질을 사용하여 이루어진다. 일반적으로, 세포 용해물 중의 효소를 비교하는 경우, 숙주 세포에 의해 생산되고, 용해물에 존재하는 효소의 양의 변동을 최소화하기 위해 동일한 발현 시스템 및 동일한 숙주 세포를 사용하는 것 뿐만 아니라, 세포의 개수 및 검정되는 단백질의 양을 측정한다.

본원에서 사용되는 바, 본원에서 사용되는 바, "전환"은 기질의 상응하는 생성물로의 효소적 변환을 지칭한다.

본원에서 사용되는 바, "전환율"은 특정 조건하에서 일정 기간 이내에 생성물로 전환되는 기질의 비율을 지칭한다. 따라서, 예를 들어, PGA 폴리펩티드의 "효소 활성" 또는 "활성"은 기질의 생성물로의 "전환율(%)"로서 표현될 수 있다.

본원에서 사용되는 바, "화학선택성"은 화학 또는 효소 반응에서 다른 것보다 한 생성물의 우선적 형성을 지칭한다.

본원에서 사용되는 바, "열안정성" 및 "열적 안정한"은 상호교환적으로 사용되고, 이는 미처리 효소와 비교하여 일정 기간(예컨대, 0.5-24 hr) 동안 온도 조건 세트(예컨대, 40-80℃)에 노출되었을 때, 불활성화에 대하여 내성을 띠고, 이로써, 상승된 온도에의 노출 후에도 일정 수준의 잔류 활성(예컨대, 60% 내지 80% 초과)을 유지하는 폴리펩티드를 지칭한다.

본원에서 사용되는 바, "용매 안정성"은 미처리 효소와 비교하여 일정 기간(예컨대, 0.5-24 hr) 동안 다양한 농도(예컨대, 5-99%)의 용매(예컨대, 이소프로필 알콜, 테트라히드로퓨란, 2-메틸테트라히드로퓨란, 아세톤, 톨루엔, 부틸아세테이트, 메틸 tert-부틸에테르 등)에의 노출 후에도 유사한 활성(예컨대, 60% 내지 80% 초과)을 유지할 수 있는 폴리펩티드의 능력을 지칭한다.

본원에서 사용되는 바, "pH 안정성"은 미처리 효소와 비교하여 일정 기간(예컨대, 0.5-24 hr) 동안 높거나 또는 낮은 pH(예컨대, 4.5-6 또는 8 내지 12)에의 노출 후도 유사한 활성(예컨대, 60% 내지 80% 초과)을 유지하는 PGA 폴리펩티드를 지칭한다.

본원에서 사용되는 바, "열 및 용매 안정성"은 열안정성이자, 용매 안정성인 PGA 폴리펩티드를 지칭한다.

본원에서 사용되는 바, "친수성 아미노산 또는 잔기"는 (Eisenberg et al.)(문헌 [Eisenberg et al., J. Mol. Biol., 179:125-142 [1984]])의 정규화된 공통 소수성 척도에 따라 0 미만인 소수성을 나타내는 측쇄를 가지는 아미노산 또는 잔기를 지칭한다. 유전적으로 코딩되는 친수성 아미노산으로는 L-Thr(T), L-Ser(S), L-His(H), L-Glu(E), L-Asn(N), L-Gln(Q), L-Asp(D), L-Lys(K) 및 L-Arg(R)를 포함한다.

본원에서 사용되는 바, "산성 아미노산 또는 잔기"는 아미노산이 펩티드 또는 폴리펩티드에 포함될 때 약 6 미만인 pK 값을 나타내는 측쇄를 가지는 친수성 아미노산 또는 잔기를 지칭한다. 산성 아미노산은 전형적으로 수소 이온의 손실로 인해 생리학적 pH에서 음으로 하전된 측쇄를 가진다. 유전적으로 코딩되는 산성 아미노산으로는 L-Glu(E) 및 L-Asp(D)를 포함한다.

본원에서 사용되는 바, "염기성 아미노산 또는 잔기"는 아미노산이 펩티드 또는 폴리펩티드에 포함될 때 약 6 초과인 pK 값을 나타내는 측쇄를 가지는 친수성 아미노산 또는 잔기를 지칭한다. 염기성 아미노산은 전형적으로 히드로늄 이온과의 회합에 기인하여 생리적 pH에서 양으로 하전된 측쇄를 가진다. 유전적으로 코딩되는 염기성 아미노산으로는 L-Arg(R) 및 L-Lys(K)을 포함한다.

본원에서 사용되는 바, "극성 아미노산 또는 잔기"는 생리적 pH에서 하전되지 않지만, 2개 원자가 공통으로 공유하는 전자쌍이 원자 중 하나에 의해 더 가깝제 유지되는 적어도 하나의 결합이 있는 측쇄를 가지는 친수성 아미노산 또는 잔기를 지칭한다. 유전적으로 코딩되는 극성 아미노산으로는 L-Asn(N), L-Gln(Q), L-Ser(S) 및 L-Thr(T)을 포함한다.

본원에서 사용되는 바, "소수성 아미노산 또는 잔기"는 (Eisenberg et al.)(문헌 [Eisenberg et al., J. Mol. Biol., 179:125-142 [1984]])의 정규화된 공통 소수성 척도에 따라 0 초과인 소수성을 나타내는 측쇄를 가지는 아미노산 또는 잔기를 지칭한다. 유전적으로 코딩되는 소수성 아미노산으로는 L-Pro(P), L-Ile I), L-Phe(F), L-Val(V), L-Leu(L), L-Trp(W), L-Met(M), L-Ala(A) 및 L-Tyr(Y)을 포함한다.

본원에서 사용되는 바, "방향족 아미노산 또는 잔기"는 적어도 하나의 방향족 또는 헤테로방향족 고리를 포함하는 측쇄를 가지는 친수성 또는 소수성 아미노산 또는 잔기를 지칭한다. 유전적으로 코딩되는 방향족 아미노산으로는 L-Phe(F), L-Tyr(Y) 및 L-Trp(W)를 포함한다. L-His(H)는 그의 헤테로방향족 질소 원자의 pKa에 기인하여 종종 염기성 잔기로 분류되거나, 또는 그의 측쇄가 헤테로방향족 고리를 포함함에 따라, 방향족 잔기로 분류되지만, 히스티딘은 본원에서 친수성 잔기 또는 "구속형 잔기"(이하 참조)로서 분류된다.

본원에서 사용되는 바, "구속형 아미노산 또는 잔기"는 구속형 기하학적 구조를 가지는 아미노산 또는 잔기를 지칭한다. 본원에서, 구속형 잔기로는 L-Pro(P) 및 L-His(H)를 포함한다. 히스티딘은 상대적으로 작은 이미다졸 고리를 갖고 있기 때문에, 구속형 기하학적 구조를 가진다. 프롤린 또한 5원 고리를 갖고 있기 때문에, 구속형 기하학적 구조를 가진다.

본원에서 사용되는 바, "비극성 아미노산 또는 잔기"는 2개 원자가 공통으로 공유하는 전자쌍이 일반적으로 각각의 두 원자에 의해 동일하게 유지되는 결합을 가지며, 생리적 pH에서 비하전된 측쇄(즉, 측쇄는 극성이 아니다)를 가지는 소수성 아미노산 또는 잔기를 지칭한다. 유전적으로 코딩된 비극성 아미노산으로는 L-Gly(G), L-Leu(L), L-Val(V), L-Ile(I), L-Met(M) 및 L-Ala(A)를 포함한다.

본원에서 사용되는 바, "지방족 아미노산 또는 잔기"는 지방족 탄화수소 측쇄를 가지는 소수성 아미노산 또는 잔기를 의미한다. 유전적으로 코딩되는 지방족 아미노산으로는 L-Ala(A), L-Val(V), L-Leu(L) 및 L-Ile(I)을 포함한다.

시스테인(또는 "L-Cys" 또는 "[C]")은 다른 L-Cys(C) 아미노산 또는 다른 술파닐 함유 또는 술프히드릴 함유 아미노산과 이황화 브릿지를 형성할 수 있다는 점에서 일반적인 것은 아님에 주의한다. "시스테인 유사 잔기"는 이황화 브릿지를 형성하는 데 이용가능한 술프히드릴 모이어티를 함유하는 다른 아미노산과 시스테인을 포함한다. 환원된 유리-SH 또는 산화된 이황화 브릿지 형태로 펩티드에 존재할 수 있는 L-Cys(C)(및 -SH 함유 측쇄를 가지는 다른 아미노산)의 능력은 L-Cys(C)가 펩티드에 대한 순 소수성 또는 친수성 특징에 기여하는지 여부에 영향을 준다. L-Cys(C)는 (Eisenberg)(문헌 [Eisenberg et al., 1984, 상기 문헌 동일])의 정규화된 공통 스케일에 따라 0.29인 소수성을 나타내지만, 본 개시내용의 목적을 위해, L-Cys(C)는 그 자체의 고유한 군으로 분류된다는 것을 이해하여야 한다.

본원에서 사용되는 바, "소형 아미노산 또는 잔기"는 총 3개 이하의 탄소 및/또는 헤테로원자(α-탄소 및 수소 배제)로 구성된 측쇄를 가지는 아미노산 또는 잔기를 지칭한다. 소형 아미노산 또는 잔기는 상기 정의에 따라 지방족, 비극성, 극성 또는 산성 소형 아미노산 또는 잔기로서 추가로 분류될 수 있다. 유전적으로 코딩되는 소형 아미노산으로는 L-Ala(A), L-Val(V), L-Cys(C), L-Asn(N), L-Ser(S), L-Thr(T) 및 L-Asp(D)를 포함한다.

본원에서 사용되는 바, "히드록실 함유 아미노산 또는 잔기"는 히드록실(-OH) 모이어티를 함유하는 아미노산을 의미한다. 유전적으로 코딩되는 히드록실 함유 아미노산으로 L-Ser(S), L-Thr(T) 및 L-Tyr(Y)을 포함한다.

본원에서 사용되는 바, "아미노산 차이" 및 "잔기 차이"는 참조 서열 중의 상응하는 위치에 있는 아미노산 잔기 대비 폴리펩티드 서열의 위치에 있는 아미노산 잔기의 차이를 지칭한다. 아미노산 차이의 위치는 일반적으로 본원에서 "Xn"으로 지칭되며, 여기서 n은 잔기 차이의 기초가 되는 참조 서열 중의 상응하는 위치를 지칭한다. 예를 들어, "서열 번호: 2와 비교하여 위치 X40에서의 잔기 차이"는 서열 번호: 2의 40번 위치에 상응하는 폴리펩티드 위치에 있는 아미노산 잔기의 차이를 지칭한다. 따라서, 서열 번호: 2의 참조 폴리펩티드가 40번 위치에 히스티딘을 가지면, 이때 "서열 번호: 2와 비교하여 위치 X40에서의 잔기 차이"란, 서열 번호: 2의 40번 위치에 상응하는 폴리펩티드의 위치에 히스티딘 이외의 다른 임의의 잔기의 아미노산 치환을 지칭한다. 본원의 대부분의 경우에서, 위치에서의 특정 아미노산 잔기 차이는 "XnY"로 표시되고, 여기서 "Xn"은 상기 기술된 바와 같이 상응하는 위치를 명시하고, "Y"는 조작된 폴리펩티드에서 발견되는 아미노산의 단일 문자 식별자이다(즉, 참조 폴리펩티드 중의 것과 다른 잔기). 일부 경우에서, 본 개시내용은 또한 통상의 표기법 "AnB"로 표시된 특정 아미노산 차이를 제공하고, 여기서, A는 참조 서열 중의 잔기의 단일 문자 식별자이고, "n"은 참조 서열 중의 잔기 위치의 번호이고, B는 조작된 폴리펩티드의 서열 중의 잔기 치환의 단일 문자 식별자이다. 일부 경우에서, 본 개시내용의 폴리펩티드는 잔기 차이가 참조 서열 대비로 존재하는 명시된 위치의 목록으로 표시되는, 참조 서열 대비의 하나 이상의 아미노산 잔기 차이를 포함할 수 있다. 일부 실시양태에서, 1 초과의 아미노산이 폴리펩티드의 특정 잔기 위치에 사용될 수 있는 경우, 사용될 수 있는 다양한 아미노산 잔기는 "/"로 분리되어 표시된다(예컨대, X192A/G). 일부 실시양태에서, 치환 세트 중의 치환은 세미콜론(";") 또는 슬래시("/")로 분리되어 표시된다(예컨대, 서열 번호: 8 대비 하기 치환 세트--Y27T;G71H;D74G;D484N;Q547K;Y584F;M697L을 가지는 변이체 PGA 경우). 본 개시내용은 보존적 및 비보존적 아미노산 치환 중 하나 또는 그 둘 모두를 포함하는 하나 이상의 아미노산 차이를 포함하는 조작된 폴리펩티드 서열을 포함한다. 본 개시내용의 서열 목록에 포함되는 특정 재조합 카보닉 안히드라제 폴리펩티드의 아미노산 서열은 개시 메티오닌(M) 잔기(즉, M은 잔기 위치 1을 나타낸다)를 포함한다. 그러나, 당업자는 이러한 개시 메티오닌 잔기가 생물학적 프로세싱 조구, 예컨대, 숙주 세포 또는 시험관내 번역 시스템에서 제거됨으로써 개시 메티오닌 잔기가 결여되지만, 효소의 특성은 유지되는 성숙한 단백질이 생성될 수 있다는 것을 이해한다. 결과적으로, 본원에서 사용되는 바, "서열 번호: 2 대비 위치 Xn에서의 아밈노산 잔기 차이"라는 용어는 위치 "Xn" 또는 출발 메티오닌을 결여시키도록 프로세싱된 참조 서열 중의 상응하는 위치(예컨대, 위치(X-1)n)를 의미할 수 있다.

본원에서 사용되는 바, "보존적 아미노산 치환"이라는 어구는 유사한 측쇄를 가지는 잔기의 상호교환성을 지칭하고, 따라서, 이는 전형적으로 폴리펩티드 중의 아미노산을 동일하거나 또는 유사한 것으로 정의된 부류의 아미노산 내의 아미노산으로 치환하는 것을 포함한다. 예로서 및 제한 없이, 일부 실시양태에서, 지방족 측쇄를 가지는 아미노산은 또 다른 지방족 아미노산(예컨대, 알라닌, 발린, 류신, 및 이소류신)으로 치환되고/거나; 히드록실 측쇄를 가지는 아미노산은 히드록실 측쇄를 가지는 또 다른 아미노산(예컨대, 세린 및 트레오닌)으로 치환되고/거나; 방향족 측쇄를 가지는 아미노산은 방향족 측쇄를 가지는 또 다른 아미노산(예컨대, 페닐알라닌, 티로신, 트립토판, 및 히스티딘)으로 치환되고/거나; 염기성 측쇄를 가지는 아미노산은 염기성 측쇄를 가지는 또 다른 아미노산(예컨대, 리신 및 아르기닌)으로 치환되고/거나; 산성 측쇄를 가지는 아미노산은 산성 측쇄를 가지는 또 다른 아미노산(예컨대, 아스파르트산 또는 글루탐산)으로 치환되고/거나; 소수성 또는 친수성 아미노산은 각각 또 다른 소수성 또는 친수성 아미노산으로 대체된다. 예시적인 보존적 치환은 표 1에 제공되어 있다.

본원에서 사용되는 바, "비보존적 치환"이라는 어구는 폴리펩티드 중의 아미노산을, 유의적으로 상이한 다른 측쇄 특성을 가지는 아미노산으로 치환하는 것을 지칭한다. 비보존적 치환은 정의된 군 안에서 보다는, 그 사이의 아미노산을 사용할 수 있고, (a) 치환 영역 내 펩티드 골격의 구조(예컨대, 글리신 대신 프롤린), (b) 전하 또는 소수성, 또는 (c) 측쇄의 부피에 영향을 준다. 예로서 및 제한 없이, 예시적인 비보존적 치환은 염기성 또는 지방족 아미노산으로 치환된 산성 아미노산, 소형 아미노산으로 치환된 방향족 아미노산, 및 소수성 아미노산으로 치환된 친수성 아미노산일 수 있다.

본원에서 사용되는 바, "결실"은 참조 폴리펩티드로부터의 하나 이상의 아미노산의 제거에 의한 폴리펩티드의 변형을 지칭한다. 결실은 효소 활성은 유지하고/거나, 조작된 효소의 개선된 특성은 유지하면서, 1개 이상의 아미노산, 2개 이상의 아미노산, 5개 이상의 아미노산, 10개 이상의 아미노산, 15개 이상의 아미노산, 또는 20개 이상의 아미노산, 아미노산 총 개수의 최대 10%, 또는 폴리펩티드를 구성하는 아미노산의 총 개수의 최대 20%의 제거를 포함할 수 있다. 결실은 폴리펩티드의 내부 부분 및/또는 말단 부분으로 지정될 수 있다. 다양한 실시양태에서, 결실은 연속되는 세그먼트를 포함할 수 있거나, 또는 비연속적일 수 있다.

본원에서 사용되는 바, "삽입"은 하나 이상의 아미노산의 참조 폴리펩티드에의 부가에 의한 폴리펩티드의 변형을 지칭한다. 일부 실시양태에서, 개선된 조작된 PGA 효소는 하나 이상의 아미노산의 자연적으로 발생된 PGA 폴리펩티드에의 삽입 뿐만 아니라, 하나 이상의 아미노산의 조작된 PGA 폴리펩티드에의 삽입을 포함한다. 삽입은 폴리펩티드의 내부 부분에 있을 수 있거나, 또는 카복시 또는 아미노 말단에 이루어질 수 있다. 본원에서 사용되는 바, 삽입은 당업계에 공지된 바와 같은 융합 단백질을 포함한다. 삽입부는 아미노산의 인접한 세그먼트일 수 있거나, 또는 자연적으로 발생된 폴리펩티드 중의 아미노산의 하나 이상의 것에 의해 이격되어 있을 수 있다.

"아미노산 치환 세트" 또는 "치환 세트"라는 용어는 참조 서열과 비교하여, 폴리펩티드 서열 중의 아미노산 치환 군을 지칭한다. 치환 세트는 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15개 이상의 아미노산 치환을 가질 수 있다. 일부 실시양태에서, 치환 세트는 실시예에 제공된 표에 열거된 변이체 PGA 중 임의의 것에 존재하는 아미노산 치환 세트를 지칭한다.

본원에서 사용되는 바, "단편"은 아미노 말단 및/또는 카복시 말단 결실을 가지지만, 나머지 아미노산 서열은 서열 중의 상응하는 위치와 동일한 폴리펩티드를 지칭한다. 단편은 전형적으로, 전장의 PGA 폴리펩티드, 예를 들어, 서열 번호: 2의 폴리펩티드의 약 80%, 약 90%, 약 95%, 약 98%, 또는 약 99%를 가질 수 있다. 일부 실시양태에서, 단편은 "생물학적으로 활성"이다(즉, 전장의 서열과 동일한 효소 활성을 나타낸다).

본원에서 사용되는 바, "단리된 폴리펩티드"는 그와 자연적으로 동반되는 다른 오염물질, 예컨대, 단백질, 지질 및 폴리뉴클레오티드로부터 실질적으로 분리된 폴리펩티드를 지칭한다. 상기 용어는 그의 자연적으로 발생된 환경 또는 발생 시스템(예컨대, 숙주 세포 또는 시험관내 합성)으로부터 제거되거나, 또는 정제된 폴리펩티드를 포함한다. 개선된 PGA 효소는 세포 내에 존재하거나, 세포 매질에 존재하거나, 또는 다양한 형태, 예컨대, 용해물 또는 단리된 제제로 제조될 수 있다. 따라서, 일부 실시양태에서, 본 개시내용의 조작된 PGA 폴리펩티드는 단리된 폴리펩티드일 수 있다.

본원에서 사용되는 바, "실질적으로 순수한 폴리펩티드"는 그 폴리펩티드 종이 존재하는 우세한 종인(즉, 몰 농도 또는 중량 기준으로 조성물 중 임의의 다른 개별 거대 분자 종보다 더 풍부한) 조성물을 의미하고, 대상 종이 존재하는 거대 분자 종의 적어도 약 50%(몰 농도 또는 중량% 기준)를 포함할 때 일반적으로 실질적으로 정제된 조성물이다. 일반적으로, 실질적으로 순수한 조작된 PGA 폴리펩티드 조성물은 조성물에 존재하는 모든 거대분자 종의 약 60% 이상, 약 70% 이상, 약 80% 이상, 약 90% 이상, 약 91% 이상, 약 92% 이상, 약 93% 이상, 약 94% 이상, 약 95% 이상, 약 96% 이상, 약 97% 이상, 약 98% 이상, 또는 약 99%(몰 농도 또는 중량% 기준)를 포함한다. 용매 종, 소형 분자(< 500 달톤), 및 원소 이온 종은 거대분자 종으로 간주되지 않는다. 일부 실시양태에서, 단리된 개선된 PGA 폴리펩티드는 실질적으로 순수한 폴리펩티드 조성물이다.

본원에서 사용되는 바, 핵산 또는 폴리펩티드와 관련하여 사용될 때, "이종성"이라는 용어는 유기체(예컨대, 야생형 유기체)에 의해 정상적으로 발현 및 분비되지 않는 서열을 지칭한다. 일부 실시양태에서, 상기 용어는 자연계에서 보통 발견되는 것과 서로 동일한 관계로 발견되지 않는 2 이상의 서브서열을 포함하거나, 또는 재조합적으로 조작되어 그의 발현 수준, 또는 세포 내에서의 다른 핵산 또는 다른 분자와의 물리적 관계, 또는 구조가 자연계에서 보통 발견되지 않는 서열을 포함한다. 예를 들어, 자연계에서 발견되지 않는 방식으로 배열된 비관련 유전자로부터의 2개 이상의 서열을 가지는 이종성 핵산은 전형적으로 재조합적으로 제조된다(예컨대, 발현 카세트, 예컨대, 벡터에 삽입된 프로모터 서열에 작동적으로 연결된 본 발명의 핵산 오픈 리딩 프레임(ORF: open reading frame)). 일부 실시양태에서, "이종성 폴리뉴클레오티드"는 실험실 기술에 의해 숙주 세포에 도입된 임의의 폴리뉴클레오티드를 지칭하고, 숙주 세포로부터 제거되고, 실험실 조작된 후, 이어서, 숙주 세포 내로 재도입된 폴리뉴클레오티드를 포함한다.

본원에서 사용되는 바, "적합한 반응 조건"은 본 개시내용의 PGA 폴리펩티드가 트리-페닐 아세테이트 보호기를 제거함으로써 유리 인슐린을 방출할 수 있는 생물촉매 반응 용액 중의 조건(예컨대, 효소 적재량, 기질 적재량, 보조인자 적재량, 온도, pH, 완충액, 공용매 등의 범위)을 의미한다. 예시적인 "적합한 반응 조건"은 본 개시내용에 제공되어 있고, 실시예에 의해 예시된다.

본원에서 사용되는 바, 예컨대, "화합물 적재량," "효소 적재량," "보조인자 적재량"에서 "적재량"은 반응의 출발시 반응 혼합물 중 성분의 농도 또는 양을 의미한다.

본원에서 사용되는 바, 생물촉매 매개 프로세스에서 "기질"은 생물촉매에 의한 작용을 받는 화합물 또는 분자를 지칭한다.

본원에서 사용되는 바, 생물촉매 매개 프로세스에서 "생성물"은 생물촉매의 작용 결과에 의한 화합물 또는 분자를 지칭한다.

본원에서 사용되는 바, "평형"은 본원에서 사용되는 바, 화학 또는 효소 반응의 정반응 속도 상수 및 역반응 속도 상수로 측정되는 바와 같은, 입체이성질체의 상호전환을 포함하는, 화학 또는 효소 반응(예컨대, 2개 종 A 및 B의 상호전환)의 화학 종의 정상 상태 농도를 야기시키는 프로세스를 의미한다.

본원에서 사용되는 바 "아실라제" 및 "아실트랜스퍼라제"는 상호교환적으로 사용되며, 이는 아실 기를 공여자에서 수용자에게로 전달하여 에스테르 또는 아미드를 형성할 수 있는 효소를 지칭한다. 아실라제 매개 역반응은 에스테르 또는 아미드의 가수분해를 일으킨다.

본원에서 사용되는 바, "페니실린 G" 및 "벤질페니실린"이란, (2S,5R,6R)-3,3-디메틸-7-옥소-6-(2-페닐아세트아미도)-4-티아-1-아자비사이클로[3.2.0]헵탄-2-카복실산(C₁₆H₁₈N₂O₄S)으로도 공지되어 있는 항생제를 지칭한다. 비록 일부 그람 음성 유기체 또한 이에 감수성이기는 하지만, 이는 주로 그람 양성 유기체에 효과적이다.

본원에서 사용되는 바, "페니실린 G 아실라제" 및 "PGA"는 상호교환적으로 사용되며, 이는 페니실린 G(벤질페니실린)의 페닐아세트산(PHA) 및 6-아미노페니실란산(6-APA)으로의 절단을 매개할 수 있는 능력을 가진 효소를 지칭한다. 일부 실시양태에서, PGA 활성은 모델 기질의 절단, 예를 들어, 6-니트로-3-(페닐아세트아미드)벤조산의 페닐아세트산 및 5-아미노-2-니트로-벤조산으로의 절단을 기초로 할 수 있다. PGA는 또한 아실 공여자의 아실 기를 아실 수용자에게로 전달하는 역반응을 수행할 수 있다. 본원에서 사용되는 바, PGA는 자연적으로 발생된(야생형) PGA 뿐만 아니라, 인간 조작에 의해 생성된 하나 이상의 조작된 폴리펩티드를 포함하는 비자연적으로 발생된 PGA 효소도 포함한다. 야생형 PGA 유전자는 54개의 아미노산으로 이루어진 스페이서 영역에 의해 연결된 알파 서브유니트(23.8 KDa) 및 베타 서브유니트(62.2 KDa)으로 구성된 이종이량체이다. 스페이서 영역이 존재하기 때문에, 활성 단백질을 형성하는 데 자가 프로세싱 단계가 요구된다.

본원에서 사용되는 바, "아실 공여자"는 아실 기를 아실 수용자에 제공하여 에스테르 또는 아미드를 형성하는 아실라제 기질의 일부를 지칭한다.

본원에서 사용되는 바, "아실 수용자"는 아실 공여자의 아실 기를 수용하여 에스테르 또는 아미드를 형성하는 아실라제 기질의 일부를 지칭한다.

본원에서 사용되는 바, "α 쇄 서열"은 서열 번호: 2의 27 내지 235번 위치에 있는 잔기에 상응하는(예컨대, 상기 잔기와 적어도 85%의 동일성을 가지는) 아미노산 서열을 지칭한다. 본원에서 사용되는 바, 단일 쇄 폴리펩티드는 "α 쇄 서열" 및 추가 서열(들)을 포함할 수 있다.

본원에서 사용되는 바, "β 쇄 서열"은 서열 번호: 2의 290 내지 846번 위치에 있는 잔기에 상응하는(예컨대, 상기 잔기와 적어도 85%의 동일성을 가지는) 아미노산 서열을 지칭한다. 본원에서 사용되는 바, 단일 쇄 폴리펩티드는 "β 쇄 서열" 및 추가 서열(들)을 포함할 수 있다.

본원에서 사용되는 바, 조작된 PGA 효소와 관련하여 사용될 때, "~로부터 유도된"은 조작의 기반이 된 원래의 PGA 효소, 및/또는 상기 PGA 효소를 코딩하는 유전자를 구별짓는다. 예를 들어, 서열번호 60의 조작된 PGA 효소는 다세대에 걸쳐 서열 번호: 2의 K. 시트로필라(K. citrophila) PGA 알파 쇄 및 베타 쇄를 코딩하는 유전자를 인공적으로 진화시킴으로써 수득되었다. 따라서, 상기 조작된 PGA 효소는 자연적으로 발생된 또는 야생형 PGA"로부터 유도된" 것이다.

본원에서 사용되는 바, "인슐린"은 정상 개체 내 췌장의 베타 세포에 의해 생산되는 폴리펩티드 호르몬을 지칭한다. 인슐린은 혈당 수준을 감소시킴으로써, 탄수화물 물질대사를 조절하는 데 필수적이다. 인슐린의 전신 결핍은 당뇨병을 유발한다. 인슐린은 51개의 아미노산으로 구성되고, 분자량은 대략 5800 달톤이다. 인슐린은 하나의 서브유니트내 이황화 결합 및 2개의 서브유니트간 이황화 결합을 함유하는, 2개의 펩티드 쇄("A" 및 "B"로 명명)로 구성된다. A 쇄는 21개의 아미노산으로 구성되고, B 쇄는 30개의 아미노산으로 구성된다. 2개 쇄는 고도로 정돈된 구조를 형성하고, A 및 B 쇄, 둘 모두에 수개의 알파 나선 영역이 존재한다. 단리된 쇄은 불활성이다. 용액에서, 인슐린은 단량체, 이량체 또는 육량체이다. 피하 주사에 사용되는 고농축 제제 중에서는 육량체이지만, 체액에 희석되면서 단량체가 된다. 이 정의는 1차 구조적 입체형태의 일부 또는 모두 및 자연적으로 발생된 인슐린의 생물학적 특성 중 적어도 하나를 가지는 임의의 정제된 단리된 폴리펩티드 및 프로인슐린을 포함하는 것으로 의도된다. 당형태 뿐만 아니라, 유사체(예컨대, 결실, 삽입 및/또는 치환을 가지는 폴리펩티드)를 비롯한, 천연 및 합성 유도된 인슐린을 포함하는 것으로 추가로 의도된다.

인슐린은 페닐아세테이트 공여자와 잠재적으로 반응할 수 있고, PGA에 의해 탈보호화될 수 있는 3개의 친핵성 아민을 함유한다. 이들 잔기는 29번 위치의 B쇄(B29) 상의 Lys 및 2개 N 말단 유리 아민, 1번 위치의 A 쇄(A1) 상의 Gly 및 1범 위치의 B 쇄(B1) 상의 Phe를 포함한다. 본원에서는 삼중 보호된 인슐린(인간 인슐린 상의 A1, B1, B29 잔기에 화학적으로 부착된 페닐 아세테이트)을 제공한다. PGA는 페닐아세테이트 아미드 결합에 대한 배타적인 선택성으로, 단백질의 펩티드 결합의 나머지는 온전하게 남겨두고, N-페닐아세테이트 보호된 펩티드 및 인슐린의 가수분해를 촉매화시키는 것으로 보고되었다(문헌 [Brtnik et al., Coll. Czech. Chem. Commun., 46 (8), 1983-1989 [1981]]; 및 [Wang et al. Biopolym., 25 (Suppl.), S109-S114 [1986]]).

본원에서 사용되는 바, "트리-페닐 아세테이트 보호기"란, 페닐 아실 기로 보호된 B1, B29 및 A1 위치에 3개의 1급 아민을 가지는 인슐린 분자를 지칭한다.

본원에서 사용되는 바, "디-페닐 아세테이트 보호기"란, 페닐 아실 기로 보호된 B1, B29 및/또는 A1 위치에 2개의 1급 아민을 가지는 인슐린 분자를 지칭한다.

본원에서 사용되는 바, "디-페닐 아세테이트 보호기"란, 페닐 아실 기로 보호된 B1, B29 또는 A1 위치에 1개의 1급 아민을 가지는 인슐린 분자를 지칭한다.

페니실린 G 아실라제

페니실린 아실라제는 (Sakaguchi 및 Murao) (문헌 [Sakaguchi and Murao, J. Agr.Chem. Soc. Jpn., 23:411 [1950]])에 의해 페니실리움 크리소게눔(Penicillium chrysogenum) Wisc. Q176으로부터 최초로 기술되었다. 페니실린 G 아실라제는 페니실린 G, 세팔로스포린 G, 및 관련 항생제의 측쇄에 작용하여 β 락탐 항생제 중간체인 6-아미노 페니실란산 및 7-아미노 데스-아세톡시 세팔로스포란산과 공통 부산물로서 페닐 아세트산을 생산하는 가수분해 효소이다. 이들 항생제 중간체는 반합성 항생제, 예컨대, 암피실린, 아목시실린, 클록사실린, 세팔렉신, 및 세파톡심의 잠재적인 빌딩 블록 중 하나이다.

상기 명시된 바와 같이, 페니실린 G 아실라제(PGA)는 반응식 1에 제시된 바와 같이, 구조식(I)의 짝염기를 가지는 페니실린 G의 구조식(II)의 짝염기를 가지는 6-아미노 페니실란산, 및 구조식(III)의 페닐아세트산으로의 가수분해적 절단을 촉매화할 수 있는 능력을 특징으로 한다:

.

이론에 의해 제한되지 않지만, 기질 특이성은 소수성 페닐 기의 인식과 연관이 있는 것으로 보이는 반면, 일부 PGA에서 베타 쇄의 N 말단의 세린 잔기인 친핵체는 베타 락탐 및 다양한 다른 기, 예컨대, 베타 아미노산의 수용자로서 작용한다. PGA는 또한 페니실린 G와 유사한 모델 기질을 절단하는 능력, 예를 들어, 반응식 2에 제시된 바와 같이, 구조식(IV)의 6-니트로-3-(페닐아세트아미도)벤조산(NIPAB)을 구조식(III)의 페닐아세트산 및 구조식(V)의 5-아미노-2-니트로-벤조산으로 절단할 수 있는 능력을 특징으로 할 수 있다(문헌 [Alkema et al., Anal. Biochem., 275:47-53 [1999]] 참조):

.

5-아미노-2-니트로-벤조산은 발색성을 띠기 때문에, 화학식(IV)의 기질은 PGA 활성을 측정하는 편리한 방식을 제공한다. 상기 반응 이외에도, PGA는 또한 광학적으로 순수한 tert 류신의 제조를 위한 DL-tert 류신의 동적 분할에도 사용될 수 있다(예컨대, 문헌 [Liu et al., Prep. Biochem. Biotechnol., 36:235-41 [2006]] 참조).

본 개시내용의의 PGA는 유기체 클루이베라 시트로필라(K. 시트로필라)로부터 수득된 효소를 기반으로 한다. 다른 유기체 유래의 PGA와 같이, K. 시트로필라의 PGA는 프리-프로-PGA 폴리펩티드의 단백질가수분해 프로세싱에 의해 생성되는 알파 서브유니트 및 베타 서브유니트로 구성된 이종이량체 효소이다. 신호 펩티드 및 스페이서 펩티드를 제거하면, 성숙한 이종이량체가 생성된다(예컨대, 문헌 [Barbero et al., Gene 49:69-80 [1986]] 참조). K. 시트로필라의 자연적으로 발생된 프리-프로-PGA 폴리펩티드의 아미노산 서열은 공개적으로 이용가능하고(예컨대, 진뱅크(Genbank) 수탁 번호 P07941, [gi:129551]), 이는 본원에서 서열 번호: 2로 제공된다. 자연적으로 발생된 K. 시트로필라 PGA의 알파 쇄 서열은 서열 번호: 2의 잔기 27 내지 235에 상응한다. 자연적으로 발생된 K. 시트로필라 PGA의 베타 쇄 서열은 서열 번호: 2의 잔기 290 내지 846에 상응한다. 서열 번호: 2의 잔기 1 내지 26은 신호 펩티드에 상응하고 서열 번호: 2의 잔기 236-289는 연결 프로펩티드에 상응하며, 둘 모두는 제거되어 α 쇄 서브유니트 및 β 쇄 서브유니트를 포함하는 이종이량체인 자연적으로 발생된 성숙한 PGA 효소를 생성한다.

일부 실시양태에서, 본 발명은 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288과 적어도 약 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 또는 99% 또는 그 이상의 서열 동일성을 가지는 아미노산 서열을 포함하는 조작된 PGA 폴리펩티드를 제공한다.

본 발명은 상업적 규모의 용도에 적합한 인슐린 특이적 탈아실화 생물촉매를 제공한다. 유도 진화는 A1/B1/B29-트리-페닐 아세테이트 보호기를 제거하거나, 또는 그를 인슐린에 부가할 수 있는 효율적인 아실라제 변이체를 개발하는 데 사용되었다. 본원에 제공된 PGA 변이체는 매우 다양한 아실기를 수용할 수 있고, 야생형 PGA와 비교하여, 증가된 용매 안정성, 및 개선된 열 안정성을 나타낸다. 본원에 제공된 변이체 PGA는 스페이서 영역이 없다. 따라서, 활성 효소 생성을 위한 자가 프로세싱 단계가 요구되지 않는다.

본 발명은 또한 조작된 PGA 폴리펩티드를 코딩하는 폴리뉴클레오티드를 제공한다. 일부 실시양태에서, 폴리뉴클레오티드는 유전자 발현을 제어하는 하나 이상의 이종성 조절 서열에 작동적으로 연결되어 있고, 이로써, 폴리펩티드를 발현할 수 있는 재조합 폴리뉴클레오티드가 생성된다. 조작된 PGA 폴리펩티드를 코딩하는 이종성 폴리뉴클레오티드를 함유하는 발현 구성물은 적절한 숙주 세포 내로 도입되어 상응하는 PGA 폴리펩티드를 발현할 수 있다.

다양한 아미노산에 상응하는 코돈에 관한 지식에 기인하여, 단백질 서열의 이용가능성이 대상을 코딩할 수 있는 모든 폴리뉴클레오티드에 관한 설명을 제공한다. 동일한 아미노산을 대안적 또는 동의 코돈에 의해 코딩하는 유전자 코드의 축퇴성을 통해, 모두가 본원에 개시된 개선된 PGA 효소를 코딩하는 것인, 매우 많은 핵산을 제조할 수 있다. 따라서, 특정 아미노산 서열을 확인함으로써, 당업자는 단백질의 아미노산 서열을 변이시키지 않는 방식으로 하나 이상의 코돈의 서열을 단순하게 변형시킴으로써 임의 개수의 상이한 핵산을 제조할 수 있다. 이와 관련하여, 본 개시내용은 구체적으로 가능한 코돈 선택을 기반으로 조합을 선택함으로써 제조될 수 있는 각각의 모든 가능한 폴리뉴클레오티드의 변이를 고려하고, 상기의 모든 변이는 실시예 5 및 6의 표에 제시된 아미노산 서열을 비롯한, 본원에 개시된 임의의 폴리펩티드에 대해 구체적으로 개시된 것으로 간주되어야 한다.

다양한 실시양태에서, 코돈은 바람직하게 단백질이 제조되는 숙주 세포에 적합하도록 선택된다. 예를 들어, 박테리아에서의 사용에 바람직한 코돈은 박테리아에서 유전자를 발현하는 데 사용되고, 효모에서의 사용에 바람직한 코돈은 효모에서의 발현을 위해 사용되고; 포유동물에서의 사용에 바람직한 코돈은 포유동물 세포에서의 발현을 위해 사용된다.

특정 실시양태에서, 천연 서열이 바람직한 코돈을 포함하게 되고, 바람직한 코돈의 사용이 모든 아미노산 잔기에 필요하지 않을 수 있기 때문에, PGA 폴리펩티드의 코돈 용법을 최적화하기 위해 모든 코돈이 교체될 필요는 없다. 결과적으로, PGA 효소를 코딩하는 코돈 최적화된 폴리뉴클레오티드는 전장의 코딩 영역의 코돈 위치의 약 40%, 50%, 60%, 70%, 80%로, 또는 90% 초과로 바람직한 코돈을 함유할 수 있다.

일부 실시양태에서, 폴리뉴클레오티드는 본원에 기술된 참조 조작된 PGA 폴리펩티드 중 임의의 것의 알파 쇄 및/또는 베타 쇄와 적어도 약 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 또는 99% 또는 그 이상의 서열 동일성을 가지는 아미노산 서열을 포함하는 PGA 폴리펩티드를 코딩하는 뉴클레오티드 서열을 포함한다. 따라서, 일부 실시양태에서, 폴리뉴클레오티드는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288에 기반한 참조 알파 및 베타 쇄 서열과 적어도 약 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 아미노산 서열을 코딩한다. 일부 실시양태에서, 폴리뉴클레오티드는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288의 알파 및/또는 베타 쇄 아미노산 서열을 코딩한다.

일부 실시양태에서, 폴리뉴클레오티드는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및 1288과 적어도 약 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 또는 99% 또는 그 이상의 서열 동일성을 가지는 아미노산 서열을 포함하는 PGA 폴리펩티드를 코딩하는 뉴클레오티드 서열을 포함한다. 따라서, 일부 실시양태에서, 폴리뉴클레오티드는 서열 번호: 3, 7, 13, 299, 1035, 1193, 1261, 및 1287과 적어도 약 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 아미노산 서열을 코딩한다.

일부 실시양태에서, 개선된 PGA 폴리펩티드를 코딩하는 단리된 폴리뉴클레오티드는 폴리펩티드의 개선된 활성 및/또는 발현을 제공하는 다양한 방식으로 조작되었다. 단리된 폴리뉴클레오티드의 벡터 내로의 삽입 이전에 그를 조작하는 것이 발현 벡터에 따라 바람직하거나, 또는 필요할 수 있다. 재조합 DNA 방법을 이용하여 폴리뉴클레오티드 및 핵산 서열을 변형시키는 방법은 당업계에 널리 공지되어 있다.

예를 들어, 돌연변이유발법 및 유도 진화 방법은 폴리뉴클레오티드에 쉽게 적용될 수 있고, 이로써, 발현, 스크리닝, 검정될 수 있는 변이체 라이브러리를 생성할 수 있다. 돌연변이유발법 및 유도 진화 방법은 당업계에 널리 공지되어 있다(예컨대, 미국 특허 번호 5,605,793, 5,811,238, 5,830,721, 5,834,252, 5,837,458, 5,928,905, 6,096,548, 6,117,679, 6,132,970, 6,165,793, 6,180,406, 6,251,674, 6,265,201, 6,277,638, 6,287,861, 6,287,862, 6,291,242, 6,297,053, 6,303,344, 6,309,883, 6,319,713, 6,319,714, 6,323,030, 6,326,204, 6,335,160, 6,335,198, 6,344,356, 6,352,859, 6,355,484, 6,358,740, 6,358,742, 6,365,377, 6,365,408, 6,368,861, 6,372,497, 6,337,186, 6,376,246, 6,379,964, 6,387,702, 6,391,552, 6,391,640, 6,395,547, 6,406,855, 6,406,910, 6,413,745, 6,413,774, 6,420,175, 6,423,542, 6,426,224, 6,436,675, 6,444,468, 6,455,253, 6,479,652, 6,482,647, 6,483,011, 6,484,105, 6,489,146, 6,500,617, 6,500,639, 6,506,602, 6,506,603, 6,518,065, 6,519,065, 6,521,453, 6,528,311, 6,537,746, 6,573,098, 6,576,467, 6,579,678, 6,586,182, 6,602,986, 6,605,430, 6,613,514, 6,653,072, 6,686,515, 6,703,240, 6,716,631, 6,825,001, 6,902,922, 6,917,882, 6,946,296, 6,961,664, 6,995,017, 7,024,312, 7,058,515, 7,105,297, 7,148,054, 7,220,566, 7,288,375, 7,384,387, 7,421,347, 7,430,477, 7,462,469, 7,534,564, 7,620,500, 7,620,502, 7,629,170, 7,702,464, 7,747,391, 7,747,393, 7,751,986, 7,776,598, 7,783,428, 7,795,030, 7,853,410, 7,868,138, 7,783,428, 7,873,477, 7,873,499, 7,904,249, 7,957,912, 7,981,614, 8,014,961, 8,029,988, 8,048,674, 8,058,001, 8,076,138, 8,108,150, 8,170,806, 8,224,580, 8,377,681, 8,383,346, 8,457,903, 8,504,498, 8,589,085, 8,762,066, 8,768,871, 9,593,326, 및 모든 관련 PCT 및 US 이외의 대응 특허; [Ling et al., Anal. Biochem., 254(2):157-78 [1997]]; [Dale et al., Meth. Mol. Biol., 57:369-74 [1996]]; [Smith, Ann. Rev. Genet., 19:423-462 [1985]]; [Botstein et al., Science, 229:1193-1201 [1985]]; [Carter, Biochem. J., 237:1-7 [1986]]; [Kramer et al., Cell, 38:879-887 [1984]]; [Wells et al., Gene, 34:315-323 [1985]]; [Minshull et al., Curr. Op. Chem. Biol., 3:284-290 [1999]]; [Christians et al., Nat. Biotechnol., 17:259-264 [1999]]; [Crameri et al., Nature, 391:288-291 [1998]]; [Crameri, et al., Nat. Biotechnol., 15:436-438 [1997]]; [Zhang et al., Proc. Nat. Acad. Sci. U.S.A., 94:4504-4509 [1997]]; [Crameri et al., Nat. Biotechnol., 14:315-319 [1996]]; [Stemmer, Nature, 370:389-391 [1994]]; [Stemmer, Proc. Nat. Acad. Sci. USA, 91:10747-10751 [1994]]; WO 95/22625; WO 97/0078; WO 97/35966; WO 98/27230; WO 00/42651; WO 01/75767; 및 WO 2009/152336(상기 문헌들은 모두 본원에서 참조로 포함된다) 참조).

일부 실시양태에서, 본 발명의 변이체 PGA 아실라제는 효소의 코딩된 활성을 변경시키지 않는 추가 서열을 추가로 포함한다. 예를 들어, 일부 실시양태에서, 변이체 PGA 아실라제는 정제에 유용한 또 다른 서열 또는 에피토프 태그에 연결된다.

일부 실시양태에서, 본 발명의 변이체 PGA 아실라제 폴리펩티드는 그들이 발현되는 숙주 세포(예컨대, 효모 또는 섬유상 진균 숙주 세포)에서 분비되고, 신호 펩티드(즉, 폴리펩티드의 아미노 말단에 연결되고, 코딩된 폴리펩티드를 세포 분비 경로로 유도하는 아미노산 서열)을 포함한 프리-단백질로서 발현된다.

일부 실시양태에서, 신호 펩티드는 내인성 K. 시트로필라 PGA 아실라제 신호 펩티드이다. 일부 다른 실시양태에서, 다른 K. 시트로필라 분비 단백질로부터의 신호 펩티드가 사용된다. 일부 실시양태에서, 다른 신호 펩티드가 숙주 세포 및 다른 인자들에 의존적으로 사용된다. 섬유상 진균 숙주 세포에 효과적인 신호 펩티드 코딩 영역으로는 아스퍼질러스 오리재(Aspergillus oryzae) TAKA 아밀라제, 아스퍼질러스 니거(Aspergillus niger) 중성 아밀라제, 아스퍼질러스 니거 글루코아밀라제, 리조무코르 미에헤이(Rhizomucor miehei) 아스파르트산 프로테이나제, 휴미콜라 인솔렌스(Humicola insolens) 셀룰라제, 휴미콜라 라누기노사(Humicola lanuginosa) 리파제, 티. 리에세이(T. reesei) 셀로비오히드롤라제 II로부터 수득된 신호 펩티드 코딩 영역을 포함하나, 이에 제한되지 않는다. 박테리아 숙주 세포에 대한 신호 펩티드 코딩 영역으로는 바실러스(Bacillus) NClB 11837 말토스형성 아밀라제, 바실러스 스테아로써모필러스(Bacillus stearothermophilus) 알파 아밀라제, 바실러스 리체니포르미스(Bacillus licheniformis) 섭틸리신, 바실러스 리체니포르미스 β-락타마제, 바실러스 스테아로써모필러스 중성 프로테아제(nprT, nprS, nprM), 및 바실러스 섭틸리스(Bacillus subtilis) prsA에 대한 유전자로부터 수득된 신호 펩티드 코딩 영역을 포함하나, 이에 제한되지 않는다. 일부 추가의 실시양태에서, 다른 신호 펩티드가 본 발명에서 사용된다(예컨대, 문헌 [Simonen and Palva, Microbiol. Rev., 57: 109-137 [1993](본원에서 참조로 포함된다) 참조). 효모 숙주 세포에 대해 추가로 유용한 신호 펩티드는 사카로마이세스 세레비지아에(Saccharomyces cerevisiae) 알파 인자, 사카로마이세스 세레비지아에 SUC2 인버타제에 대한 유전자로부터의 것을 포함한다(예컨대, 문헌 [Taussig and Carlson, Nucl. Acids Res., 11:1943-54 [1983]]; 스위스프로트(SwissProt) 수탁 번호 P00724; 및 [Romanos et al., Yeast 8:423-488 [1992]] 참조). 일부 실시양태에서, 이들 신호 펩티드 및 다른 신호 펩티드의 변이체가 사용된다. 실제로, 본 발명은 당업계에 공지된 임의의 적합한 신호 펩티드가 본 발명에서 사용되는 바, 임의의 특정 신호 펩티드로 제한되는 것으로 의도되지 않는다.

일부 실시양태에서, 본 발명은 본원에 기술된 바와 같이, 변이체 PGA 아실라제 폴리펩티드, 및/또는 그의 생물학적 활성 단편을 코딩하는 폴리뉴클레오티드를 제공한다. 일부 실시양태에서, 폴리뉴클레오티드는 폴리펩티드를 발현할 수 있는 재조합 폴리뉴클레오티드를 생성시키도록 유전자 발현을 제어하는 하나 이상의 이종성 조절 또는 제어 서열에 작동가능하게 연결된다. 일부 실시양태에서, 변이체 PGA 아실라제를 코딩하는 이종성 폴리뉴클레오티드를 함유하는 발현 구성물은 적절한 숙주 세포 내로 도입되어 변이체 PGA 아실라제를 발현한다.

당업계의 숙련가는 유전자 코드의 축퇴성에 기인하여, 본 발명의 변이체 PGA 아실라제 폴리펩티드를 코딩하는 다수의 뉴클레오티드 서열이 존재함을 이해한다. 예를 들어, 코돈 AGA, AGG, CGA, CGC, CGG, 및 CGU는 모두 아미노산 아르기닌을 코딩한다. 따라서, 아르기닌이 코돈에 의해 특정되는 본 발명의 핵산 내 모든 위치에서, 코돈은 코딩된 폴리펩티드를 변경시키지 않고 상기 기술된 상응하는 코돈 중 임의의 것으로 변경될 수 있다. RNA 서열의 "U"는 DNA 서열의 "T"에 상응함을 이해한다. 본 발명은 가능한 코돈 선택을 기반으로 조합을 선택하여 제조될 수 있는 본 발명의 폴리펩티드를 코딩하는 핵산 서열의 각각의 모든 가능한 변이를 고려하고, 제공한다.

상기에 나타낸 바와 같이, PGA를 코딩하는 DNA 서열은 또한 높은 코돈 용법 편향 코돈(동일한 아미노산을 코딩하는 다른 코돈보다 단백질 코딩 영역에서 더 높은 빈도로 사용되는 코돈)에 대해 디자인될 수도 있다. 바람직한 코돈은 단일 유전자에서 코돈 용법, 공통 기능 또는 기원의 유전자 세트, 고도로 발현되는 유전자, 전체 유기체의 집합 단백질 코딩 영역 내 코돈 빈도, 관련 유기체의 집합 단백질 코딩 영역 내 코돈 빈도, 또는 그의 조합과 관련하여 결정될 수 있다. 그 빈도가 유전자 발현도에 따라 증가하는 코돈은 전형적으로 발현에 최적인 코돈이다. 특히, DNA 서열은 특정 숙주 유기체에서의 발현을 위해 최적화될 수 있다. 다변량 분석(예컨대, 군집 분석 또는 대응 분석을 사용) 및 유전자에 사용되는 코돈의 유효수를 비롯한, 특정 유기체에서 코돈 빈도(예컨대, 코돈 용법, 상대 동의 코돈 용법) 및 코돈 선호도를 결정하는 다양한 방법이 당업계에 널리 공지되어 있다. 코돈 용법을 수득하기 위한 데이터 공급원은 단백질을 코딩할 수 있는 임의의 이용가능한 뉴클레오티드 서열에 의존할 수 있다. 이들 데이터 세트는 발현되는 단백질을 코딩하는 것으로 실제로 알려진 핵산 서열(예컨대, 완전한 단백질 코딩 서열-CDS), 발현된 서열 태그(ESTS), 또는 게놈 서열의 예상되는 코딩 영역을 포함한다. 변이체 PGA를 코딩하는 폴리뉴클레오티드는 당업계에 공지된 임의의 적합한 방법을 사용해 제조될 수 있다. 전형적으로, 올리고뉴클레오티드는 개별적으로 합성된 후, (예컨대, 효소적 또는 화학적 라이게이션 방법, 또는 폴리머라제 매개 방법에 의해) 연결되어 본질적으로 임의의 바람직한 연속되는 서열을 형성한다. 일부 실시양태에서, 본 발명의 폴리뉴클레오티드는 자동화 합성 방법을 포함하나, 이에 제한되지 않는, 당업계에 공지된 임의의 적합한 방법을 사용하여 화학적 합성에 의해 제조된다. 예를 들어, 포스포르아미다이트 방법에서, 올리고뉴클레오티드가 합성되고(자동화 DNA 합성기에서), 정제, 어닐링, 라이게이션되고, 적절한 벡터에 클로닝된다. 일부 실시양태에서, 이중 가닥 DNA 단편은 상보성 가닥을 합성하고, 적절한 조건하에서 함께 가닥들을 어닐링하거나, 또는 적절한 프라이머 서열과 함께 DNA 폴리머라제를 사용하여 상보성 가닥을 부가하여 수득된다. 본 발명에서 유용한 방법을 제공하는 수많은 일반 및 표준 교재가 당업자에게 널리 공지되어 있다.

조작된 PGA는 상기 논의된 바와 같이, 돌연변이유발법 및/또는 유도 진화 방법을 자연적으로 발생된 PGA를 코딩하는 폴리뉴클레오티드에 수행함으로써 수득될 수 있다. 돌연변이유발법은 무작위 및 부위 지정 돌연변이유발법을 비롯한, 당업계에 공지된 기술 중 임의의 것에 따라 수행될 수 있다. 유도 진화는 셔플링을 비롯한, 개선된 변이체에 대해 스크리닝하는 당업계에 공지된 기술 중 임의의 것으로 수행될 수 있다. 사용되는 다른 유도 진화 방법으로는 스태거드 확장 프로세스(StEP: staggered extension process), 시험관내 재조합법, 돌연변이성 PCR, 카세트 돌연변이유발법, 중첩 연장에 의한 스플라이싱(SOEing: splicing by overlap extension), ProSAR™ 유도 진화 방법 등 뿐만 아니라, 임의의 다른 적합한 방법을 포함하나, 이에 제한되지 않는다.

돌연변이유발 처리 후 수득된 클론은 원하는 개선된 효소 특성을 가지는 조작된 PGA에 대해 스크리닝된다. 발현 라이브러리로부터의 효소 활성 측정은 생성물 형성 속도를 모니터링하는 표준 생화학 기술을 사용하여 수행될 수 있다. 원하는 개선된 효소 특성이 열 안정성인 경우, 효소 활성은 효소 제제를 정의된 온도에 가하고, 열처리 후 남은 효소 활성의 양을 측정한 후에 측정될 수 있다. 이어서, PGA를 코딩하는 폴리뉴클레오티드를 함유하는 클론을 단리시키고, 서열분석하여 (있다면) 뉴클레오티드 서열 변이를 확인하고, 숙주 세포에서 효소를 발현시키는 데 사용한다.

조작된 폴리펩티드의 서열이 공지되어 있는 경우, 효소를 코딩하는 폴리뉴클레오티드는 공지된 합성 방법에 따라, 표준 고체상 방법으로 제조될 수 있다. 일부 실시양태에서, 최대 약 100개 염기로 이루어진 단편은 개별적으로 합성된 후, (예컨대, 효소적 또는 화학적 라이게이션 방법, 또는 폴리머라제 매개 방법에 의해) 연결되어 임의의 원하는 연속되는 서열을 형성할 수 있다. 예를 들어, 본 발명의 폴리뉴클레오티드 및 올리고뉴클레오티드는 (예컨대, 전형적으로 자동화 합성 방법에서 실시되는 바와 같이, 문헌 [Beaucage et al., Tet. Lett., 22:1859-69 [1981]]에 기술된 고전적 포스포르아미다이트 방법, 또는 [Matthes et al., EMBO J., 3:801-05 [1984]]에 기술된 방법을 사용하여) 화학 합성에 의해 제조될 수 있다. 포스포르아미다이트 방법에 따라, 올리고뉴클레오티드가 합성되고(자동화 DNA 합성기에서), 정제, 어닐링, 라이게이션되고, 적절한 벡터에 클로닝된다. 추가로, 본질적으로 임의의 핵산은 임의의 다양한 상업적 공급처에서 수득할 수 있다(예컨대, The Midland Certified Reagent Company: 미국 텍사스주 미들랜드 소재, The Great American Gene Company: 미국 캘리포니아주 라모나 소재, ExpressGen Inc.: 미국 일리노이주 시카고 소재, Operon Technologies Inc.: 미국 캘리포니아주 앨러미다 소재 및 다수의 다른 공급처).

본 발명은 또한 본원에 제공된 바와 같이, 적어도 하나의 변이체 PGA를 코딩하는 서열을 포함하는 재조합 구성물을 제공한다. 일부 실시양태에서, 본 발명은 이종성 프로모터에 작동가능하게 연결된 변이체 PGA 폴리뉴클레오티드를 포함하는 발현 벡터를 제공한다. 일부 실시양태에서, 본 발명의 발현 벡터는 숙주 세포가 변이체 PGA 단백질을 발현할 수 있게 허용하는 적절한 숙주 세포를 형질전환시키는 데 사용된다. 진균 및 다른 유기체에서의 단백질의 재조합 발현을 위한 방법은 당업계에 널리 공지되어 있고, 다수의 발현 벡터가 이용가능하거나, 또는 통상의 방법을 사용하여 구성될 수 있다. 일부 실시양태에서, 본 발명의 핵산 구성물은 본 발명의 핵산 서열이 삽입된, 벡터, 예컨대, 플라스미드, 코스미드, 파지, 바이러스, 박테리아 인공 염색체(BAC: bacterial artificial chromosome), 효소 인공 염색체(YAC: yeast artificial chromosome) 등을 포함한다. 일부 실시양태에서, 본 발명의 폴리뉴클레오티드는 변이체 PGA 폴리펩티드(들)를 발현하는 데 적합한 다양한 발현 벡터 중 어느 하나의 것 도입된다. 적합한 벡터로는 염색체, 비염색체 및 합성 DNA 서열(예컨대, SV40의 유도체) 뿐만 아니라, 박테리아 플라스미드, 파지 DNA, 배큘로바이러스, 효모 플라스미드, 플라스미드와 파지 DNA의 조합으로부터 유도된 벡터, 바이러스 DNA, 예컨대, 백시니아, 아데노바이러스, 계두 바이러스, 가성광견병, 아데노바이러스, 아데노 연관 바이러스, 레트로바이러스, 및 다수의 다른 것들을 포함하나 이에 제한되지 않는다. 세포에 유전 물질을 형질전환시키고, 복제가 바람직하면, 관련 숙주에서 복제가능하고 생존가능한 임의의 적합한 벡터가 본 발명에서 사용된다. 일부 실시양태에서, 구성물은 단백질 코딩 서열에 작동가능하게 연결된 프로모터를 포함하나, 이에 제한되지 않는 조절 서열을 추가로 포함한다. 다수의 적합한 벡터 및 프로모터가 당업자에게 공지되어 있다. 실제로, 일부 실시양태에서, 특정 숙주에서 높은 발현 수준을 수득하기 위해, 종종 이종성 프로모터의 제어하에 본 발명의 변이체 PGA를 발현시키는 것이 유용하다. 일부 실시양태에서, 프로모터 서열은 당업계에 공지된 임의의 적합한 방법을 사용해 변이체 PGA 코딩 서열의 5' 영역에 작동가능하게 연결된다. 변이체 PGA의 발현을 위해 유용한 프로모터의 예로는 진균으로부터의 프로모터를 포함하나, 이에 제한되지 않는다. 일부 실시양태에서, 진균 균주의 PGA 유전자 이외의 유전자의 발현을 구동시키는 프로모터 서열이 사용된다. 비제한적인 예로서, 엔도글루카나제를 코딩하는 유전자로부터의 진균 프로모터가 사용될 수 있다. 일부 실시양태에서, PGA의 유래 기점이 된 진균 균주 이외의 다른 진균 균주에서의 PGA 유전자 발현을 구동시키는 프로모터 서열이 사용된다. 섬유상 진균 숙주 세포에서 본 발명의 뉴클레오티드 구성물의 전사를 지시하는 데 유용한 다른 적합한 프로모터의 예로는 아스퍼질러스 오리재 TAKA 아밀라제, 리조무코르 미에헤이 아스파르트산 프로테이나제, 아스퍼질러스 니거 중성 알파 아밀라제, 아스퍼질러스 니거 산 안정 알파 아밀라제, 아스퍼질러스 니거 또는 아스퍼닐러스 아와모리(Aspergillus awamori) 글루코아밀라제(glaA), 리조무코르 미에헤이 리파제, 아스퍼질러스 오리재 알칼리성 프로테아제, 아스퍼질러스 오리재 트리오스 포스페이트 이소머라제, 아스퍼질러스 니둘란스(Aspergillus nidulans) 아세트아미다제, 및 푸사리움 옥시스포럼(Fusarium oxysporum) 트립신 유사 프로테아제(예컨대, WO 96/00787(본원에서 참조로 포함된다) 참조)에 대한 유전자로부터 수득된 프로모터 뿐만 아니라, NA2-tpi 프로모터(아스퍼질러스 니거 중성 알파 아밀라제 및 아스퍼질러스 오리재 트리오스 포스페이트 이소머라제에 대한 유전자 유래의 프로모터의 하이브리드), 프로모터, 예컨대, cbh1, cbh2, egl1, egl2, pepA, hfb1, hfb2, xyn1, amy, 및 glaA(예컨대, 문헌 [Nunberg et al., Mol. Cell Biol., 4:2306 -2315 [1984]]; [Boel et al., EMBO J., 3:1581-85 [1984]]; 및 유럽 특허 출원 137280(상기 문헌 모두 본원에서 참조로 포함된다), 및 그의 돌연변이체, 말단절단형, 및 하이브리드 프로모터를 포함하나, 이에 제한되지 않는다.

효모 숙주 세포에서, 유용한 프로모터로는 사카로마이세스 세레비지아에 에놀라제(eno-1), 사카로마이세스 세레비지아에 갈락토키나제(gal1), 사카로마이세스 세레비지아에 알콜 데히드로게나제/글리세르알데히드-3-포스페이트 데히드로게나제(ADH2/GAP), 및 S. 세레비지아에(S. cerevisiae) 3-포스포글리세레이트 키나제에 대한 유전자로부터의 것을 포함하나, 이에 제한되지 않는다. 효모 숙주 세포에 유용한 추가의 유용한 프로모터는 당업계에 공지되어 있다(예컨대, 문헌 [Romanos et al., Yeast 8:423-488 [1992]](본원에서 참조로 포함된다) 참조). 추가로, 진균에서의 키티나제 생산과 연관된 프로모터가 본 발명에서 사용된다(예컨대, 문헌 [Blaiseau and Lafay, Gene 120243-248 [1992]]; [Limon et al., Curr. Genet., 28:478-83 [1995]](상기 두 문헌 모두 본원에서 참조로 포함된다) 참조).

박테리아 숙주 세포의 경우, 본 개시내용의 핵산 구성물의 전사를 지시하는 데 적합한 프로모터로는 이. 콜라이 lac 오페론, 이. 콜라이 trp 오페론, 박테리오파지 λ, 스트렙토마이세스 코엘리컬러(Streptomyces coelicolor) 아가라제 유전자(dagA), 바실러스 섭틸리스 레반수크라제 유전자(sacB), 바실러스 리체니포르미스 알파 아밀라제 유전자(amyL), 바실러스 스테아로써모필러스 말토스형성 아밀라제 유전자(amyM), 바실러스 아밀로리케파시엔스(Bacillus amyloliquefaciens) 알파 아밀라제 유전자(amyQ), 바실러스 리체니포르미스 페니실리나제 유전자(penP), 바실러스 섭틸리스 xylA 및 xylB 유전자, 및 원핵생물 베타 락타마제 유전자로부터 수득된 프로모터(예컨대 문헌 [Villa-Kamaroff et al., Proc. Natl. Acad. Sci. USA 75: 3727-3731 [1978]] 참조) 뿐만 아니라, tac 프로모터(예컨대 문헌 [DeBoer et al., Proc. Natl. Acad. Sci. USA 80: 21-25 [1983]] 참조)를 포함하나, 이에 제한되지 않는다.

일부 실시양태에서, 본 발명의 클로닝된 변이체 PGA는 또한 전사를 종결하기 위해 숙주 세포에 의해 인식되는 서열인, 적합한 전사 종결인자 서열을 가진다. 종결인자 서열은 폴리펩티드를 코딩하는 핵산 서열의 3' 말단에 작동가능하게 연결된다. 선택된 숙주 세포에서 기능성인 임의의 종결인자가 본 발명에서 사용된다. 섬유상 진균 숙주 세포에 대한 예시적인 전사 종결인자로는 아스퍼질러스 오리재 TAKA 아밀라제, 아스퍼질러스 니거 글루코아밀라제, 아스퍼질러스 니둘란스 안트라닐레이트 시타제, 아스퍼질러스 니거 알파 글루코시다제, 및 푸사리움 옥시스포럼 트립신 유사 프로테아제에 대한 유전자로부터 수득된 것을 포함하나, 이에 제한되지 않는다(또한, 미국 특허 번호 7,399,627(본원에서 참조로 포함된다) 참조). 일부 실시양태에서, 효모 숙주 세포에 대한 예시적인 종결인자로는 사카로마이세스 세레비지아에 에놀라제, 사카로마이세스 세레비지아에 시토크롬 C(CYCl), 및 사카로마이세스 세레비지아에 글리세르알데히드-3-포스페이트 데히드로게나제에 대한 유전자로부터 수득된 것들을 포함한다. 효모 숙주 세포에 대한 다른 유용한 종결인자는 당업자에게 널리 공지되어 있다(예컨대, 문헌 [Romanos et al., Yeast 8:423-88 [1992]] 참조).

일부 실시양태에서, 적합한 리더 서열은 숙주 세포에 의한 번역에 중요한 mRNA의 비번역 영역인, 클로닝된 변이체 PGA 서열의 일부이다. 리더 서열은 폴리펩티드를 코딩하는 핵산 서열의 5' 말단에 작동가능하게 연결된다. 선택된 숙주 세포에서 기능성인 임의의 리더 서열이 본 발명에서 사용된다. 섬유상 진균 숙주 세포에 대한 예시적인 리더로는 아스퍼질러스 오리재 TAKA 아밀라제 및 아스퍼질러스 니둘란스 트리오스 포스페이트 이소머라제에 대한 유전자로부터 수득된 것들을 포함하나, 이에 제한되지 않는다. 효모 숙주 세포에 대해 적합한 리더로는 사카로마이세스 세레비지아에 에놀라제(ENO-1), 사카로마이세스 세레비지아에 3-포스포글리세레이트 키나제, 사카로마이세스 세레비지아에 알파 인자, 및 사카로마이세스 세레비지아에 알콜 데히드로게나제/글리세르알데히드-3-포스페이트 데히드로게나제(ADH2/GAP)에 대한 유전자로부터 수득된 것들을 포함하나, 이에 제한되지 않는다.

일부 실시양태에서, 본 발명의 서열은 또한 핵산 서열의 3' 말단에 작동가능하게 연결된 서열이고, 전사시, 전사된 mRNA에 폴리아데노신 잔기를 부가하는 신호로서 숙주 세포에 의해 인식되는, 폴리아데닐화 서열을 포함한다. 선택된 숙주 세포에서 기능성인 임의의 폴리아데닐화 서열이 본 발명에서 사용된다. 섬유상 진균 숙주 세포에 대한 예시적인 폴리아데닐화 서열로는 아스퍼질러스 오리재 TAKA 아밀라제, 아스퍼질러스 니거 글루코아밀라제, 아스퍼질러스 니둘란스 안트라닐레이트 시타제, 푸사리움 옥시스포럼 트립신 유사 프로테아제, 및 아스퍼질러스 니거 알파 글루코시다제에 대한 유전자로부터 수득된 것들을 포함하나, 이에 제한되지 않는다. 효모 숙주 세포에 대한 유용한 폴리아데닐화 서열은 당업계에 공지되어 있다(예컨대, 문헌 [Guo and Sherman, Mol. Cell. Biol., l5:5983-5990 [1995]] 참조).

일부 실시양태에서, 제어 서열은 폴리펩티드의 아미노 말단에 연결된 아미노산 서열을 코딩하는 신호 펩티드 코딩 영역을 포함하고, 코딩된 폴리펩티드를 세포의 분비 경로로 유도한다. 핵산 서열의 코딩 서열의 5' 단부는 본질적으로, 분비되는 폴리펩티드를 코딩하는 코딩 영역의 세그먼트와 함께 번역 리딩 프레임내에서 자연적으로 연결된 신호 펩티드 코딩 영역을 함유할 수 있다. 대안적으로, 코딩 서열의 5' 단부는 코딩 서열에 외래적인 신호 펩티드 코딩 영역을 함유할 수 있다. 외래 신호 펩티드 코딩 영역은 코딩 서열이 자연적으로 신호 펩티드 코딩 영역을 함유하지 않는 경우에 요구될 수 있다.

대안적으로, 외래 신호 펩티드 코딩 영역은 폴리펩티드의 분비를 강화시키기 위해 천연 신호 펩티드 코딩 영역을 단순히 교체할 수 있다. 그러나, 발현된 폴리펩티드를 선택된 숙주 세포의 분비 경로로 유도하는 임의의 신호 펩티드 코딩 영역이 본 발명에 사용될 수 있다.

박테리아 숙주 세포에 대해 효과적인 신호 펩티드 코딩 영역으로는 바실러스 NClB 11837 말토스형성 아밀라제, 바실러스 스테아로써모필러스 알파 아밀라제, 바실러스 리체니포르미스 섭틸리신, 바실러스 리체니포르미스 베타 락타마제, 바실러스 스테아로써모필러스 중성 프로테아제(nprT, nprS, nprM), 및 바실러스 섭틸리스 prsA에 대한 유전자로부터 수득된 신호 펩티드 코딩 영역을 포함하나, 이에 제한되지 않는다. 추가의 신호 펩티드는 당업계에 공지되어 있다(예컨대, 문헌 [Simonen and Palva, Microbiol. Rev., 57: 109-137 [1993]] 참조).

섬유상 진균 숙주 세포에 대해 효과적인 신호 펩티드 코딩 영역으로는 아스퍼질러스 오리재 TAKA 아밀라제, 아스퍼질러스 니거 중성 아밀라제, 아스퍼질러스 니거 글루코아밀라제, 리조무코르 미에헤이 아스파르트산 프로테이나제, 휴미콜라 인솔렌스 셀룰라제, 및 휴미콜라 라누기노사 리파제에 대한 유전자로부터 수득된 신호 펩티드 코딩 영역을 포함하나, 이에 제한되지 않는다.

효모 숙주 세포에 유용한 신호 펩티드는 사카로마이세스 세레비지아에 알파 인자 및 사카로마이세스 세레비지아에 인버타제에 대한 유전자를 포함하나, 이에 제한되지 않는다. 다른 유용한 신호 펩티드 코딩 영역은 당업계에 공지되어 있다(예컨대, 문헌 [Romanos et al., [1992], 상기 문헌 동일] 참조).

일부 실시양태에서, 제어 서열은 폴리펩티드의 아미노 말단에 위치하는 아미노산 서열을 코딩하는 프로펩티드 코딩 영역을 포함한다. 생성된 폴리펩티드는 프로효소 또는 프로폴리펩티드(또는 일부 경우에서 자이모겐)로 알려져 있다. 프로폴리펩티드는 일반적으로 불활성이고, 프로폴리펩티드로부터 프로펩티드의 촉매성 또는 자가촉매성 절단에 의해 성숙한 활성 PGA 폴리펩티드로 전환될 수 있다. 프로펩티드 코딩 영역은 바실러스 섭틸리스 알칼리성 프로테아제(aprE), 바실러스 섭틸리스 중성 프로테아제(nprT), 사카로마이세스 세레비지아에 알파 인자, 리조무코르 미에헤이 아스파르트산 프로테이나제, 및 마이셀리오프토라 써모필라(Myceliophthora thermophila) 락타제에 대한 유전자로부터 수득될 수 있다(예컨대, WO 95/33836 참조).

신호 펩티드 및 프로펩티드 영역, 둘 모두가 폴리펩티드의 아미노 말단에 존재하는 경우, 프로펩티드 영역은 폴리펩티드의 아미노 말단 다음에 위치하고, 신호 펩티드 영역은 프로펩티드 영역의 아미노 말단 다음에 위치한다.

일부 실시양태에서, 조절 서열은 또한 숙주 세포의 성장 대비 폴리펩티드의 발현이 조절될 수 있도록 하기 위해 사용된다. 조절 시스템의 예는 조절 화합물의 존재를 비롯한, 화학적 또는 물리적 자극에 대응하여 유전자의 발현을 작동시키거나, 또는 작동시키지 않는 것이다. 원핵생물 숙주 세포에서, 적합한 조절 서열로는 lac, tac, 및 trp 오퍼레이터 시스템을 포함하나, 이에 제한되지 않는다. 효모 숙주 세포에서, 적합한 조절 시스템은 예로서, ADH2 시스템 또는 GAL1 시스템을 포함한다. 섬유상 진균에서, 적합한 조절 서열은 AKA 알파 아밀라제 프로모터, 아스퍼질러스 니거 글루코아밀라제 프로모터, 및 아스퍼질러스 오리재 글루코아밀라제 프로모터를 포함한다.

조절 서열의 다른 예는 유전자 증폭을 가능하게 하는 것들이다. 진핵생물 시스템에서, 이들은 메토트렉세이트의 존재에서 증폭되는, 디히드로폴레이트 리덕타제 유전자, 및 중금속으로 증폭되는, 메탈로티오네인 유전자를 포함한다. 이러한 경우에서, 본 발명의 PGA 폴리펩티드를 코딩하는 핵산 서열은 조절 서열과 함께 작동가능하게 연결될 것이다.

따라서, 추가 실시양태에서, 본 발명은 조작된 PGA 폴리펩티드 또는 그의 변이체를 코딩하는 폴리뉴클레오티드, 및 그들이 도입되는 숙주의 유형에 따라서, 하나 이상의 발현 조절 영역, 예컨대, 프로모터 및 종결인자, 복제 기점 등을 포함하는 재조합 발현 벡터를 제공한다. 일부 실시양태에서, 상기 기술된 다양한 핵산 및 제어 서열은 함께 연결되어 하나 이상의 편리한 제한효소 부위를 포함하여 그 부위에서 폴리펩티드를 코딩하는 핵산 서열의 삽입 또는 치환을 가능하게 할 수 있는 재조합 발현 벡터가 생성된다. 대안적으로, 일부 실시양태에서, 핵산 서열은 발현을 위해 적절한 벡터에 핵산 서열 또는 서열을 포함하는 핵산 구성물을 삽입시켜 발현된다. 발현 벡터 생성시, 코딩 서열은 상기 코딩 서열이 발현을 위해 적절한 제어 서열과 작동가능하게 연결되도록 벡터에 위치한다.

재조합 발현 벡터는 편리하게 재조합 DNA 방법에 따라 수행될 수 있고, 폴리뉴클레오티드 서열을 발현시킬 수 있는, 임의의 적합한 벡터(예컨대, 플라스미드 또는 바이러스)를 포함한다. 벡터의 선택은 전형적으로 벡터가 도입되는 숙주 세포와 벡터의 상용성에 의존한다. 일부 실시양태에서, 벡터는 선형이거나, 또는 폐쇄된 고리형 플라스미드이다.

일부 실시양태에서, 발현 벡터는 자가 복제 벡터(즉, 염색체외 엔티티로서 존재하고, 그 복제가 염색체 복제와는 독립적인 벡터, 예컨대, 플라스미드, 염색체외 요소, 미니염색체 또는 인공 염색체)이다. 일부 실시양태에서, 벡터는 자가 복제를 보장하는 임의 수단을 함유한다. 대안적으로, 일부 다른 실시양태에서, 숙주 세포 내로의 도입시, 벡터는 게놈 내로 통합되고, 그가 내부에 통합되어 염색체(들)와 함께 복제된다. 추가로, 추가 실시양태에서, 단일 벡터 또는 플라스미드 또는 숙주 세포의 게놈에 도입되는 전체 DNA를 함께 함유하는 2개 이상의 벡터 또는 플라스미드, 또는 트랜스포존이 사용된다.

일부 실시양태에서, 본 발명의 발현 벡터는 형질전환된 세포의 용이한 선별을 가능하게 하는, 하나 이상의 선별가능한 마커를 함유한다. "선별가능한 마커"는 그의 생성물이 살생물성 또는 바이러스 내성, 항미생물제 또는 중금속에 대한 내성, 영양요구성에 대해 자가영양성을 제공하는 유전자이다. amdS(아세트아미다제), argB(오르니틴 카바모일트랜스퍼라제), bar(포스피노트리신 아세틸트랜스퍼라제), hph(히그로마이신 포스포트랜스퍼라제), niaD(니트레이트 리덕타제), pyrG(오로티딘-5'-포스페이트 데카복실라제), sC(술페이트 아데닐트랜스퍼라제), 및 trpC(안트라닐레이트 시타제) 뿐만 아니라, 그의 등가물을 포함하나, 이에 제한되지 않는, 섬유상 진균 숙주 세포에 사용하기 적합한 임의의 선별가능한 마커가 본 발명에서 사용된다. 예컨대, 아스퍼질러스와 같은 숙주 세포에서 유용한 추가의 마커로는 아스퍼질러스 니둘란스 또는 아스퍼질러스 오리재의 amdS 및 pyrG 유전자, 및 스트렙토마이세스 히그로스코피커스(Streptomyces hygroscopicus)의 bar 유전자를 포함하나, 이에 제한되지 않는다. 효모 숙주 세포에 적합한 마커로는 ADE2, HIS3, LEU2, LYS2, MET3, TRP1, 및 URA3을 포함하나, 이에 제한되지 않는다. 박테리아 선별가능한 마커의 예로는 바실러스 섭틸리스 또는 바실러스 리체니포르미스로부터의 dal 유전자, 또는 항생제 내성, 예컨대, 암피실린, 카나마이신, 클로람페니콜, 및 또는 테트라사이클린 내성을 부여하는 마커를 포함하나, 이에 제한되지 않는다.

일부 실시양태에서, 본 발명의 발현 벡터는 숙주 세포의 게놈 내로의 벡터의 통합 또는 게놈과 독립적으로 세포 내에서 벡터의 자가 복제를 가능하게 하는 성분(들)을 함유한다. 숙주 세포 게놈에의 통합을 포함하는 일부 실시양태에서, 벡터는 폴리펩티드를 코딩하는 핵산 서열 또는 상동성 또는 비상동성 재조합에 의해 게놈 내로의 벡터의 통합을 위한 벡터의 임의의 다른 성분에 의존한다.

일부 대안적인 실시양태에서, 발현 벡터는 상동성 재조합에 의한 숙주 세포의 게놈 내로의 통합을 지시하기 위한 추가의 핵산 서열을 함유한다. 추가의 핵산 서열을 통해 벡터는 염색체(들) 내 정확한 위치(들)에서 숙주 세포 게놈 내로 통합될 수 있다. 정확한 위치에 통합될 수 있는 가능성을 증가시키기 위해, 통합 요소는 바람직하게 상동성 재조합의 확률을 증가시키기 위해 상응하는 표적 서열과 고도의 상동성인, 충분한 개수의 뉴클레오티드, 예컨대, 100 내지 10,000개의 염기쌍, 바람직하게, 400 내지 10,000개의 염기쌍, 가장 바람직하게 800 내지 10,000개의 염기쌍을 함유한다. 통합 요소는 숙주 세포의 게놈 내 표적 서열과 상동성인 임의 서열일 수 있다. 추가로, 통합 요소는 비코딩 또는 코딩 핵산 서열일 수 있다. 한편, 벡터는 비상동성 재조합에 의해 숙주 세포의 게놈 내로 통합될 수 있다.

자가 복제를 위해, 벡터는 해당 숙주 세포에서 벡터가 자가 복제할 수 있도록 하는 복제 기점을 추가로 포함할 수 있다. 박테리아 복제 기점의 예로는 P15A ori 또는 이. 콜라이에서 복제할 수 있게 하는 플라스미드 pBR322, pUC19, pACYCl77(이 플라스미드는 P15A ori를 가진다), 또는 pACYC184, 및 바실러스에서 복제할 수 있게 하는 pUB110, pE194, pTA1060, 또는 pAMβ1의 복제 기점이 있다. 효모 숙주 세포에서 사용하기 위한 복제 기점의 예로는 2 미크론 복제 기점, ARS1, ARS4, ARS1 및 CEN3의 조합, 및 ARS4 및 CEN6의 조합이 있다. 복제 기점은 숙주 세포에서 그의 기능성을 온도 감수성으로 만드는 돌연변이를 가지는 것일 수 있다(예컨대, 문헌 [Ehrlich, Proc. Natl. Acad. Sci. USA 75:1433 [1978]] 참조).

일부 실시양태에서, 유전자 생성물의 제조를 증가시키기 위해 본 발명의 핵산 서열의 1개 초과의 카피를 숙주 세포 내로 삽입시킨다. 핵산 서열의 카피 수 증가는 서열의 적어도 하나의 추가 카피를 숙주 세포 게놈 내로 통합시키거나, 또는 선별가능한 마커 유전자의 증폭된 카피와, 그에 따라 핵산 서열의 추가 카피를 함유하는 세포가 적절한 선별제의 존재하에서 세포를 배양하는 것에 의해 선택될 수 있는 핵산 서열과 증폭가능한 선별가능한 마커 유전자를 포함시켜 수득될 수 있다.

본 발명에서 사용하기 위한 다수의 발현 벡터는 상업적으로 이용가능하다. 적합한 상업적 발현 벡터로는 포유동물 숙주 세포에서의 발현을 위한 CMV 프로모터 및 hGH 폴리아데닐화 부위 및 이. 콜라이에서의 증폭을 위한 pBR322 복제 기점 및 암피실린 내성 마커를 포함하는, p3xFLAGTM™ 발현 벡터(Sigma-Aldrich Chemicals)를 포함하나, 이에 제한되지 않는다. 다른 적합한 발현 벡터로는 pBluescriptII SK(-) 및 pBK-CMV(Stratagene), 및 pBR322(Gibco BRL), pUC(Gibco BRL), pREP4, pCEP4(Invitrogen) 또는 pPoly(예컨대, 문헌 [Lathe et al., Gene 57:193-201 [1987]] 참조)로부터 유래된 플라스미드를 포함하나, 이에 제한되지 않는다.

따라서, 일부 실시양태에서, 적어도 하나의 변이체 PGA를 코딩하는 서열을 포함하는 벡터는 벡터의 증식 및 변이체 PGA(들)의 발현을 가능하게 하기 위해 숙주 세포로 형질전환된다. 일부 실시양태에서, 변이체 PGA는 신호 펩티드를 제거하도록 번역 후 변형되고, 일부 경우에서 분비 후 절단될 수 있다. 일부 실시양태에서, 상기 기술된 형질전환된 숙주 세포는 변이체 PGA(들)의 발현을 가능하게 하는 조건하에 적합한 영양 배지 중에서 배양된다. 최소 배지, 또는 적절한 보충제를 함유하는 복합 배지를 포함하나, 이에 제한되지 않는, 숙주 세포를 배양하는 데 유용한 임의의 적합한 배지가 본 발명에서 사용된다. 일부 실시양태에서, 숙주 세포는 HTP 배지 중에서 성장된다. 적합한 배지는 다양한 상업적 공급처에서 입수할 수 있거나, 또는 (예컨대, 미국 모식균 배양 수집소(American Type Culture Collection)의 카탈로그에의) 공개된 제조법에 따라 제조될 수 있다.

또 다른 측면에서, 본 발명은 본원에 제공된 개선된 PGA 폴리펩티드를 코딩하는 폴리뉴클레오티드를 포함하는 숙주 세포로서, 여기서, 폴리뉴클레오티드는 숙주 세포에서 PGA 효소의 발현을 위해 하나 이상의 제어 서열에 작동적으로 연결되어 있는 것인 숙주 세포를 제공한다. 본 발명의 발현 벡터에 의해 코딩되는 PGA 폴리펩티드를 발현하는 데 사용하기 위한 숙주 세포는 당업계에 널리 공지되어 있고, 박테리아 세포, 예컨대, 이. 콜라이, 바실러스 메가테리움(Bacillus megaterium), 락토바실러스 케피르(Lactobacillus kefir), 스트렙토마이세스(Streptomyces) 및 살모넬라 티피뮤리움(Salmonella typhimurium) 세포; 진균 세포, 예컨대, 효모 세포(예컨대, 사카로마이세스 세레비지아에 또는 피치아 파스토리스(Pichia pastoris)(ATCC 수탁 번호 201178)); 곤충 세포, 예컨대, 드로소필라(Drosophila) S2 및 스포돕테라(Spodoptera) Sf9 세포; 동물 세포, 예컨대, CHO, COS, BHK, 293, 및 보우스 흑색종 세포; 및 식물 세포를 포함하나, 이에 제한되지 않는다. 상기 기술된 숙주 세포에 적절한 배양 배지 및 성장 조건은 당업계에 널리 공지되어 있다.

PGA의 발현을 위한 폴리뉴클레오티드는 당업계에 공지된 다양한 방법에 의해 세포 내로 도입될 수 있다. 기술로는 특히 전기천공, 바이오리스틱스 입자 충격, 리포솜 매개 형질감염, 염화칼슘 형질감염, 원형질체 융합을 포함한다. 세포에 폴리뉴클레오티드를 도입시키기 위한 다양한 방법이 당업자에게 공지되어 있다.

일부 실시양태에서, 숙주 세포는 진핵생물 세포이다. 적합한 진핵생물 숙주 세포로는 진균 세포, 조류 세포, 곤충 세포, 및 식물 세포를 포함하나, 이에 제한되지 않는다. 적합한 진균 숙주 세포로는 아스코마이코타(Ascomycota), 바시디오마이코타(Basidiomycota), 듀테로마이코타(Deuteromycota), 자이고마이코타(Zygomycota), 불완전 진균류를 포함하나, 이에 제한되지 않는다.. 일부 실시양태에서, 진균 숙주 세포는 효모 세포 및 섬유상 진균 세포이다. 본 발명의 섬유상 진균 숙주 세포는 유마이코티나(Eumycotina) 아문 및 우마이코타(Oomycota) 아문의 모든 섬유상 형태를 포함한다. 섬유상 진균은 키틴, 셀룰로스 및 다른 복합 다당류로 구성된 세포벽을 가지는 영양 균사체를 특징으로 한다. 본 발명의 섬유상 진균 숙주 세포는 효모와 형태학적으로 구별된다.

본 발명의 일부 실시양태에서, 섬유상 진균 숙주 세포아클리아(Achlya), 아크레모니움(Acremonium), 아스퍼질러스, 아우레오바시디움(Aureobasidium), 비제르칸데라(Bjerkandera), 세리포리옵시스(Ceriporiopsis), 세팔로스포리움(Cephalosporium), 크리소스포리움(Chrysosporium), 코클리오보러스(Cochliobolus), 코리나스커스(Corynascus), 크리포넥트리아(Cryphonectria), 크립토코커스(Cryptococcus), 코프리너스(Coprinus), 코리오러스(Coriolus), 디플로디아(Diplodia), 엔도티스(Endothis), 푸사리움(Fusarium), 지베렐라(Gibberella), 글리오클라디움(Gliocladium), 휴미콜라(Humicola), 히포크레아(Hypocrea), 마이셀리오프토라(Myceliophthora), 무코르(Mucor), 뉴로스포라(Neurospora), 페니실리움(Penicillium), 포도스포라(Podospora), 플레비아(Phlebia), 피로마이세스(Piromyces), 피리쿨라리아(Pyricularia), 리조무코르(Rhizomucor), 리조퍼스(Rhizopus), 스키조필럼(Schizophyllum), 스시탈리디움(Scytalidium), 스포로트리컴(Sporotrichum), 탈라로마이세스(Talaromyces), 써모아스커스(Thermoascus), 티에라비아(Thielavia), 트라메테스(Trametes), 톨리포클라디움(Tolypocladium), 트리코더마(Trichoderma), 베르티실리움(Verticillium), 및/또는 볼바리엘라(Volvariella), 및/또는 그의 유성생식형, 또는 무성생식형, 및 이명, 기본명, 또는 분류상 등가물을 포함하나, 이에 제한되지 않는, 임의의 속 및 종의 것이다.

본 발명의 일부 실시양태에서, 숙주 세포는 칸디다(Candida), 한세눌라(Hansenula), 사카로마이세스, 스키조사카로마이세스(Schizosaccharomyces), 피치아, 클루이베로마이세스(Kluyveromyces), 또는 야로위아(Yarrowia) 종의 세포를 포함하나, 이에 제한되지 않는 효모 세포이다. 본 발명의 일부 실시양태에서, 효모 세포는 한세눌라 폴리몰파(Hansenula polymorpha), 사카로마이세스 세레비지아에, 사카로마이세스 칼스베르겐시스(Saccharomyces carlsbergensis), 사카로마이세스 디아스타티커스(Saccharomyces diastaticus), 사카로마이세스 놀벤시스(Saccharomyces norbensis), 사카로마이세스 클루이베리(Saccharomyces kluyveri), 스키조사카로마이세스 폼베(Schizosaccharomyces pombe), 피치아 파스토리스, 피치아 핀란디카(Pichia finlandica), 피치아 트레할로필라(Pichia trehalophila), 피치아 코다마에(Pichia kodamae), 피치아 멤브라내파시엔스(Pichia membranaefaciens), 피치아 오푼티아에(Pichia opuntiae), 피치아 써모톨레란스(Pichia thermotolerans), 피치아 살리크타리아(Pichia salictaria), 피치아 케르큠(Pichia quercuum), 피치아 피지페리(Pichia pijperi), 피치아 스티피티스(Pichia stipitis), 피치아 메타놀리카(Pichia methanolica), 피치아 안구스타(Pichia angusta), 클루이베로마이세스 락티스(Kluyveromyces lactis), 칸디다 알비칸스(Candida albicans), 또는 야로위아 리포리티카(Yarrowia lipolytica)이다.

본 발명의 일부 실시양태에서, 숙주 세포는 조류 세포, 예컨대, 클라미도모나스(Chlamydomonas)(예컨대, C. 레인하르드티이(C. reinhardtii)) 및 포르미디움(Phormidium)(P. sp. ATCC29409)이다.

일부 다른 실시양태에서, 숙주 세포는 원핵생물 세포이다. 적합한 원핵생물 세포로는 그람 양성, 그람 음성 및 그람 가변성(Gram-variable) 박테리아 세포를 포함하나, 이에 제한되지 않는다. 아그로박테리움(Agrobacterium), 알리사이클로바실러스(Alicyclobacillus), 아나바에나(Anabaena), 아나시스티스(Anacystis), 아시네토박터(Acinetobacter), 악시도써머스(Acidothermus), 알트로박터(Arthrobacter), 아조박터(Azobacter), 바실러스, 비피도박테리움(Bifidobacterium), 브레비박테리움(Brevibacterium), 부티리비브리오(Butyrivibrio), 부치네라(Buchnera), 캄페스트리스(Campestris), 캠플리오박터(Camplyobacter), 클로스트리디움(Clostridium), 코리네박테리움(Corynebacterium), 클로마티움(Chromatium), 코프로코커스(Coprococcus), 에스케리키아(Escherichia), 엔테로코커스(Enterococcus), 엔테로박터(Enterobacter), 어위니아(Erwinia), 푸소박테리움(Fusobacterium), 파에칼리박테리움(Faecalibacterium), 프란시셀라(Francisella), 플라보박테리움(Flavobacterium), 지오바실러스(Geobacillus), 해모필러스(Haemophilus), 헬리코박터(Helicobacter), 클렙시엘라(Klebsiella), 락토바실러스(Lactobacillus), 락토코커스(Lactococcus), 일리오박터(Ilyobacter), 마이크로코커스(Micrococcus), 마이크로박테리움(Microbacterium), 메소리조비움(Mesorhizobium), 메틸로박테리움(Methylobacterium), 메틸로박테리움(Methylobacterium), 마이코박테리움(Mycobacterium), 나이세리아(Neisseria), 판토에아(Pantoea), 슈도모나스(Pseudomonas), 프로클로로코커스(Prochlorococcus), 로도박터(Rhodobacter), 로도슈도모나스(Rhodopseudomonas), 로도슈도모나스(Rhodopseudomonas), 로세버리아(Roseburia), 로도스피릴럼(Rhodospirillum), 로도코커스(Rhodococcus), 세네데스머스(Scenedesmus), 스트렙토마이세스, 스트렙토코커스(Streptococcus), 시네코커스(Synecoccus), 사카로모노스포라(Saccharomonospora), 스타필로코커스(Staphylococcus), 세라티아(Serratia), 살모넬라, 시겔라(Shigella), 써모아나에로박테리움(Thermoanaerobacterium), 트로페리마(Tropheryma), 툴라렌시스(Tularensis), 테메쿨라(Temecula), 써모시네코코커스(Thermosynechococcus), 써모코커스(Thermococcus), 우레아플라스마(Ureaplasma), 크산토모나스(Xanthomonas), 크실렐라(Xylella), 여시니아(Yersinia) 및 자이모모나스(Zymomonas)를 포함하나, 이에 제한되지 않는 임의의 적합한 박테리아 유기체가 본 발명에서 사용된다. 일부 실시양태에서, 숙주 세포는 아그로박테리움, 아시네토박터, 아조박터, 바실러스, 비피도박테리움, 부치네라, 지오바실러스, 캄필로박터, 클로스트리디움, 코리네박테리움, 에스케리키아, 엔테로코커스, 어위니아, 플라보박테리움, 락토바실러스, 락토코커스, 판토에아, 슈도모나스, 스타필로코커스, 살모넬라, 스트렙토코커스, 스트렙토마이세스, 또는 자이모모나스의 종이다. 일부 실시양태에서, 박테리아 숙주 균주는 인간에 비병원성이다. 일부 실시양태에서 박테리아 숙주 균주는 산업용 균주이다. 다수의 박테리아 산업용 균주가 공지되어 있고, 본 발명에 적합하다. 본 발명의 일부 실시양태에서, 박테리아 숙주 세포는 아그로박테리움 종(예컨대, A. 라디오박터(A. radiobacter), A. 리조게네스(A. rhizogenes), 및 A. 루비(A. rubi))이다. 본 발명의 일부 실시양태에서, 박테리아 숙주 세포는 알트로박터 종(예컨대, A. 아우레센스(A. aurescens), A. 시트레우스(A. citreus), A. 글로비포르미스(A. globiformis), A. 히드로카르보글루타미커스(A. hydrocarboglutamicus), A. 미소렌스(A. mysorens), A. 니코티아나에(A. nicotianae), A. 파라피네우스(A. paraffineus), A. 프로토폰니아에(A. protophonniae), A. 로세오파르크피너스(A. roseoparqffinus), A. 술퍼레우스(A. sulfureus), 및 A. 우레아파시엔스(A. ureafaciens))이다. 본 발명의 일부 실시양태에서, 박테리아 숙주 세포는 바실러스 종(예컨대, B. 투린겐시스(B. thuringensis), B. 안트라시스(B. anthracis), B. 메가테리움, B. 섭틸리스, B. 렌터스(B. lentus ), B. 실쿨란스(B. circulans), B. 푸밀러스(B. pumilus), B. 라우터스(B. lautus), B. 코아굴란스(B. coagulans), B. 브레비스(B. brevis), B. 필머스(B. firmus), B. 알카오피우스(B. alkaophius), B. 리체니포르미스, B. 클라우시이(B. clausii), B. 스테아로써모필러스, B. 할로두란스(B. halodurans), 및 B. 아밀로리케파시엔스)이다. 일부 실시양태에서, 숙주 세포는 B. 섭틸리스, B. 푸밀러스, B. 리체니포르미스, B. 메가테리움, B. 클라우시이, B. 스테아로써모필러스, 또는 B. 아밀로리케파시엔스를 포함하나, 이에 제한되지 않는 산업용 바실러스 균주이다. 일부 실시양태에서, 바실러스 숙주 세포는 B. 섭틸리스, B. 리체니포르미스, B. 메가테리움, B. 스테아로써모필러스, 및/또는 B. 아밀로리케파시엔스이다. 일부 실시양태에서, 박테리아 숙주 세포는 클로스트리디움 종(예컨대, C. 아세토부틸리컴(C. acetobutylicum), C. 테타니(C. tetani) E88, C. 리투세부렌스(C. lituseburense), C. 사카로부틸리컴(C. saccharobutylicum), C. 펄프린겐스(C. perfringens), 및 C. 베이제린키이(C. beijerinckii))이다. 일부 실시양태에서, 박테리아 숙주 세포는 코리네박테리움 종(예컨대, C. 글루타미컴(C. glutamicum) 및 C. 아세토아시도필럼(C. acetoacidophilum))이다. 일부 실시양태에서, 박테리아 숙주 세포는 에스케리키아 종(예컨대, 이. 콜라이)이다. 일부 실시양태에서, 박테리아 숙주 세포는 어위니아 종(예컨대, 예컨대, E. 우레도보라(E. uredovora), E. 카로토보라(E. carotovora), E. 아나나스(E. ananas), E. 헬비콜라(E. herbicola), E. 푼타타(E. punctata), 및 E. 테레우스(E. terreus))이다. 일부 실시양태에서, 박테리아 숙주 세포는 판토에아 종(예컨대, P. 시트레아(P. citrea), 및 P. 아글로메란스(P. agglomerans))이다. 일부 실시양태에서, 박테리아 숙주 세포는 슈도모나스 종(예컨대, P. 푸티다(P. putida), P. 아에루기노사(P. aeruginosa), P. 메발로니이(P. mevalonii), 및 P. sp. D-01 10)이다. 일부 실시양태에서, 박테리아 숙주 세포는 스트렙토코커스 종(예컨대, S. 에퀴시밀레스(S. equisimiles), S. 피오게네스(S. pyogenes), 및 S. 우베리스(S. uberis))이다. 일부 실시양태에서, 박테리아 숙주 세포는 스트렙토마이세스 종(예컨대, S. 암보파시엔스(S. ambofaciens), S. 아크로모게네스(S. achromogenes), S. 아벨미틸리스(S. avermitilis), S. 코엘리컬러(S. coelicolor), S. 아우레오파시엔스(S. aureofaciens), S. 아우레우스(S. aureus), S. 푼지시디커스(S. fungicidicus), S. 그리세우스(S. griseus), 및 S. 리비단스(S. lividans))이다. 일부 실시양태에서, 박테리아 숙주 세포는 자이모모나스 종(예컨대, 종(예컨대, Z. 모빌리스(Z. mobilis), 및 Z. 리포리티카(Z. lipolytica))이다.

예시적인 숙주 세포는 에스케리키아 콜라이 W3110이다. 발현 벡터는 lacI 리프레서의 제어하에 있는 lac 프로모터에 작동적으로 연결된 플라스미드 pCK110900에, 개선된 PGA를 코딩하는 폴리뉴클레오티드를 작동적으로 연결시킴으로써 생성하였다. 발현 벡터는 또한 P15a 복제 기점 및 클로람페니콜 내성 유전자를 함유하였다. 에스케리키아 콜라이 W3110에 대상 폴리뉴클레오티드를 함유하는 세포는 상기 세포에 대하여 클로람페니콜 선별을 수행함으로써 단리시켰다.

본 발명에서 사용되는 다수의 원핵생물 및 진핵생물 균주는 다수의 배양 수집소, 예컨대, 미국 모식균 배양 수집소(ATCC: American Type Culture Collection), 독일 생물자원센터(DSM: Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH), 사균 배지 중앙 보관소(CBS: Centraalbureau Voor Schimmelcultures) 및 농업 연구소 배양 보관소(Agricultural Research Service Patent Culture Collection), 북방 연구 센터(NRRL: Northern Regional Research Center)를 통해 공중에 의해 용이하게 입수될 수 있다.

일부 실시양태에서, 숙주 세포는 단백질 분비, 단백질 안정성 및/또는 단백질의 발현 및/또는 분비에 바람직한 다른 특성을 개선시키는 특징을 가지도록 유전적으로 변형된다. 유전자 변형은 유전자 조작 기술 및/또는 고전적인 미생물학적 기술(예컨대, 화학적 또는 UV 돌연변이유발법 및 후속 선별법)에 의해 달성될 수 있다. 실제로, 일부 실시양태에서, 재조합 변형 및 고전적 선별 기술의 조합을 사용하여 숙주 세포를 제조한다. 재조합 기술을 사용하여, 숙주 세포 내에서 및/또는 배양 배지 중에서 PGA 변이체(들)의 수율을 증가시키는 방식으로 핵산 분자를 도입시키거나, 결실시키거나, 억제시키거나 또는 변형시킬 수 있다. 예를 들어, Alp1 기능의 넉아웃은 프로테아제 결핍된 세포를 초래하고, pyr5 기능의 넉아웃은 피리미딘 결핍 표현형을 가지는 세포를 초래한다. 한 유전자 조작 접근법에서, 상동성 재조합은 코딩된 단백질의 발현을 억제시키기 위하여 생체내에서 유전자를 특이적으로 표적화하여 표적화된 유전자 변형을 유도하는 데 사용된다. 대안적 접근법에서, siRNA, 안티센스 및/또는 리보자임 기술이 유전자 발현을 억제하는 데 사용된다. 세포에서 단백질의 발현을 감소시키기 위한 것으로서, 단백질을 코딩하는 유전자 모두 또는 일부를 결실시키는 것, 및 유전자 생성물의 발현 또는 활성을 파괴시키는 부위 특이적 돌연변이유발법을 포함하나, 이에 제한되지 않는 다양한 방법이 당업계에 공지되어 있다(예컨대, 문헌 [Chaveroche et al., Nucl. Acids Res., 28:22 e97 [2000]]; [Cho et al., Molec. Plant Microbe Interact., 19:7-15 [2006]]; [Maruyama and Kitamoto, Biotechnol Lett., 30:1811-1817 [2008]]; [Takahashi et al., Mol. Gen. Genom., 272: 344-352 [2004]]; 및 [You et al., Arch. Micriobiol.,191:615-622 [2009]](상기 문헌들은 모두 본원에서 참조로 포함된다) 참조). 무작위 돌연변이유발법, 이어서, 바람직한 돌연변이에 스크리닝 또한 사용된다(예컨대, 문헌 [Combier et al., FEMS Microbiol . Lett ., 220:141-8 [2003]]; 및 [Firon et al., Eukary. Cell 2:247-55 [2003]](상기 두 문헌 모두 본원에서 참조로 포함된다) 참조).

벡터 또는 DNA 구성물의 숙주 세포 내로의 도입은 인산칼슘 형질감염, DEAE-덱트스란 매개 형질감염, PEG-매개 형질전환, 전기천공, 또는 당업계에 공지된 다른 공통 기술을 포함하나, 이에 제한되지 않는, 당업계에 공지된 임의의 적합한 방법을 사용하여 달성될 수 있다.

일부 실시양태에서, 본 발명의 조작된 숙주 세포(즉, "재조합 숙주 세포")는 프로모터를 활성화시키거나, 형질전환체를 선별하거나, 또는 PGA 폴리뉴클레오티드를 증폭시키기에 적절하게 변형된 통상의 영양 배지에서 배양된다. 배양 조건, 예컨대, 온도, pH 등은 발현을 위해 선택된 숙주 세포에 따라 이전에 사용된 것들이고, 당업자에게 널리 공지되어 있다. 언급한 바와 같이, 다수의 표준 참고문헌 및 교재가 박테리아, 식물, 동물(특히, 포유동물) 및 고세균 기원의 세포를 비롯한, 많은 세포의 배양 및 제조를 위해 이용가능하다.

일부 실시양태에서, 본 발명의 변이체 PGA 폴리펩티드를 발현하는 세포는 회분식 또는 연속형 발효 조건하에서 성장된다. 고전적인 "회분식 발효"는 폐쇄 시스템으로서, 여기서, 배지의 조성은 발효의 시작시에 설정되어 있고, 발효 동안 인공적인 변경이 가해지지 않는다. 회분식 시스템의 변형은 본 발명에서 또한 사용되는 "유가식 발효"이다. 상기 변형에서는 발효가 진행됨에 따라 기질이 증분량으로 첨가된다. 유가식 시스템은 이화대사 산물 억제가 아마도 세포의 물질대사를 억제할 수 있는 경우 및 배지 내에 제한된 양의 기질을 가지는 것이 바람직한 경우에 유용하다. 회분식 및 유가신 발효는 당업계에서 일반적이고, 널리 공지되어 있다. "연속 발효"는 정해진 발효 배지가 연속적으로 생물반응기에 첨가되고, 동량의 조건화 배지가 프로세싱 동안 동시에 제거되는 개방형 시스템이다. 연속 발효는 일반적으로 세포를 주로 대수기 성장의 일정한 고밀도로 배양물을 유지시킨다. 연속 발효 시스템은 정상 상태 성장 조건을 유지시키도록 노력한다. 연속 발효 프로세스를 위해 영양분 및 성장 인자를 조정하기 위한 방법 뿐만 아니라, 생성물 형성 속도를 최대화하기 위한 기술이 산업 미생물학 분야에 널리 공지되어 있다.

본 발명의 일부 실시양태에서, 무세포 전사/번역 시스템이 변이체 PGA(들)를 제조하는 데 사용된다. 수개의 시스템은 상업적으로 이용가능하고, 방법은 당업자에게 널리 공지되어 있다.

본 발명은 변이체 PGA 폴리펩티드, 또는 그의 생물학적 활성 단편을 제조하는 방법을 제공한다. 일부 실시양태에서, 본 방법은 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288과 적어도 약 70%(또는 적어도 약 75%, 적어도 약 80%, 적어도 약 85%, 적어도 약 90%, 적어도 약 95%, 적어도 약 96%, 적어도 약 97%, 적어도 약 98%, 또는 적어도 약 99%)의 서열 동일성을 포함하는 아미노산 서열을 코딩하고, 본원에 제공된 바와 같은 적어도 하나의 돌연변이를 포함하는 폴리뉴클레오티드로 형질전환된 숙주 세포를 제공하는 단계; 숙주 세포가 코딩된 변이체 PGA 폴리펩티드를 발현하는 조건하에서 형질전환된 숙주 세포를 배양 배지 중에서 배양하는 단계; 및 임의적으로, 발현된 변이체 PGA 폴리펩티드를 회수 또는 단리시키는 단계, 및/또는 발현된 변이체 PGA 폴리펩티드를 함유하는 배양 배지를 회수 또는 단리시키는 단계를 포함한다. 일부 실시양태에서, 본 방법은 임의적으로 코딩된 PGA 폴리펩티드를 발현시킨 후, 형질전환된 숙주 세포를 용해시키는 단계 및 임의적으로 세포 용해물로부터 발현된 변이체 PGA 폴리펩티드를 회수 및/또는 단리시키는 단계를 추가로 제공한다. 본 발명은 변이체 PGA 폴리펩티드 제조에 적합한 조건하에서 변이체 PGA 폴리펩티드로 형질전환된 숙주 세포를 배양하는 단계 및 변이체 PGA 폴리펩티드를 회수하는 단계를 포함하는, 변이체 PGA 폴리펩티드를 제조하는 방법을 추가로 제공한다. 전형적으로, PGA 폴리펩티드의 회수 또는 단리는 본원에 기술된 것들을 포함하여, 당업계에 널리 공지된 단백질 회수 기술을 사용하여, 숙주 세포 배양 배지, 숙주 세포 또는 그 둘 모두로부터 수행한다. 일부 실시양태에서, 숙주 세포는 원심분리에 의해 수확되고, 물리적 또는 화학적 수단에 의해 파괴되고, 생성된 조 추출물은 추가 정제를 위해 유지된다. 단백질의 발현에 사용된 미생물 세포는 냉동-해동 사이클링, 초음파처리, 기계적 파괴, 및/또는 세포 용해제의 사용을 포함하나, 이에 제한되지 않는 임의의 편리한 방법 뿐만 아니라, 당업자에게 널리 공지된 다수의 다른 적합한 방법에 의해 파괴시킬 수 있다.

숙주 세포에서 발현되는 조작된 PGA 효소는 특히, 리소자임 처리, 초음파처리, 여과, 염석, 초원심분리, 및 크로마토그래피를 비롯한, 단백질 정제를 위한 것으로 널리 공지된 기술 중 어느 하나 이상의 것을 사용하여 세포 및/또는 배양 배지로부터 회수될 수 있다. 박테리아, 예컨대, 이. 콜라이로부터 용해 및 단백질의 고효율 추출을 위해 적합한 용액은 상품명 셀라이틱스 B(CelLytic B)™(Sigma-Aldrich)하에 상업적으로 이용가능하다. 따라서, 일부 실시양태에서, 생성된 폴리펩티드는 당업계에 공지된 다수의 방법들 중 임의의 것에 의해 회수/단리되고, 임의적으로, 정제된다. 예를 들어, 일부 실시양태에서, 폴리펩티드는 원심분리, 여과, 추출, 분무 건조, 증발, 크로마토그래피(예컨대, 이온 교환, 친화성, 소수성 상호작용, 크로마토포커싱, 및 크기 배제), 또는 침전을 포함하나, 이에 제한되지 않는 통상의 방법에 의해 영양 배지로부터 단리된다. 일부 실시양태에서, 단백질 리폴딩 단계는 원하는 경우, 성숙한 단백질의 배열 완료시에 사용된다. 추가로, 일부 실시양태에서, 고성능 액체 크로마토그래피(HPLC: high performance liquid chromatography)가 최종 정제 단계에서 사용된다. 예를 들어, 일부 실시양태에서, 당업계에 공지된 방법이 본 발명에서 사용된다(예컨대, 문헌 [Parry et al., Biochem. J., 353:117 [2001]]; 및 [Hong et al., Appl. Microbiol. Biotechnol., 73:1331 [2007]](상기 두 문헌 모두 본원에서 참조로 포함된다) 참조). 실제로, 당업계에 공지된 임의의 적합한 정제 방법이 본 발명에서 사용된다.

PGA 폴리펩티드의 단리를 위한 크로마토그래피 기술로는 역상 크로마토그래피, 고성능 액상 크로마토그래피, 이온 교환 크로마토그래피, 겔 전기영동, 및 친화성 크로마토그래피를 포함하나, 이에 제한되지 않는다. 특정 효소를 정제하기 위한 조건은 부분적으로는 당업자에게 공지되어 있는 인자들, 예컨대, 순전하, 소수성, 친수성, 분자량, 분자 형상 등에 의존할 것이다.

일부 실시양태에서, 친화성 기술이 개선된 PGA 효소를 단리시키는 데 사용된다. 친화성 크로마토그래피 정제를 위해, PGA 폴리펩티드에 특이적으로 결합하는 임의의 항체가 사용될 수 있다. 항체 제조를 위해, 토끼, 마우스. 래트 등을 포함하나, 이에 제한되지 않는 각종 숙주 동물을 PGA의 주사에 의해 면역화시킬 수 있다. PGA 폴리펩티드를 측쇄 작용기 또는 측쇄 작용기에 부착된 링커에 의해, 적합한 담체, 예컨대, BSA에 부착시킬 수 있다. 프로인트(완전 및 불완전), 미네랄 겔, 예컨대, 수산화알루미늄, 계면 활성 물질, 예컨대, 리소레시틴, 플루로닉 폴리올, 다가 음이온, 펩티드, 오일 에멀션, 키홀 림펫 헤모시아닌, 디니트로페놀, 및 잠재적으로 유용한 인간 애주번트, 예컨대, BCG(바실러스 칼메-구에린: Bacillus Calmette Guerin) 및 코리네박테리움 파르붐(Corynebacterium parvum)을 포함하나, 이에 제한되지 않는, 각종의 애주번트가 숙주 종에 따라, 면역학적 반응을 증가시키는 데 사용될 수 있다.

일부 실시양태에서, PGA 변이체는 효소를 발현하는 세포의 형태로, 조 추출물로서, 또는 단리된 또는 정제된 제제로서 제조되고, 사용된다. 일부 실시양태에서, PGA 변이체는 분말 형태(예컨대, 아세톤 분말)인, 동결건조물로서 제조되거나, 또는 효소 용액으로 제조된다. 일부 실시양태에서, PGA 변이체는 실질적으로 순수한 제제 형태이다.

일부 실시양태에서, PGA 폴리펩티드는 임의의 적합한 고체 기판에 부착된다. 고체 기판으로는 고체상, 표면, 및/또는 막을 포함하나, 이에 제한되지 않는다. 고체 지지체로는 유기 중합체, 예컨대, 폴리스티렌, 폴리에틸렌, 폴리프로필렌, 폴리플루오로에틸렌, 폴리에틸렌옥시, 및 폴리아크릴아미드 뿐만 아니라, 그의 공중합체 및 그래프트를 포함하나, 이에 제한되지 않는다. 고체 지지체는 또한 무기물, 예컨대, 유리, 실리카, 공극 조절형 유리(CPG: controlled pore glass), 역상 실리카 또는 금속, 예컨대, 금 또는 백금일 수 있다. 기판의 입체형태는 비드, 구체 입자, 과립, 겔, 막 또는 표면의 형태일 수 있다. 표면은 평면, 실질적으로 평면 또는 비평면일 수 있다. 고체 지지체는 다공성이거나, 또는 비다공성일 수 있고, 팽윤성 또는 비팽윤성 특징을 가질 수 있다. 고체 지지체는 웰, 함몰형, 또는 다른 용기, 베쓸, 지형, 또는 위치 형태로 구성될 수 있다. 다수의 지지체가 시약의 로봇식 전달을 다룰 수 있거나, 또는 검출 방법 및/또는 장비에 의해 다룰 수 있는, 다양한 위치의 어레이 상에 배치될 수 있다.

일부 실시양태에서, 면역학적 방법이 PGA 변이체를 정제하는 데 사용된다. 한 접근법에서, 통상의 방법을 사용하여 변이체 PGA 폴리펩티드에 대하여(예컨대, 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 12882 중 임의의 것을 포함하는 폴리펩티드, 및/또는 그의 면역원성 단편에 대하여) 생성된 항체를 비드 상에 고정화시키고, 변이체 PGA가 결합하는 조건하에서 세포 배양 배지와 혼합하고, 침전시킨다. 관련된 접근법에서, 면역크로마토그래피가 사용된다.

일부 실시양태에서, 변이체 PGA는 비효소 부분을 포함한 융합 단백질로서 발현된다. 일부 실시양태에서, 변이체 PGA 서열은 정제 용이 도메인에 융합된다. 본원에서 사용되는 바, "정제 용이 도메인"이라는 용어는 융합되는 폴리펩티드의 정제를 매개하는 도메인을 지칭한다. 적합한 정제 도메인으로는 금속 킬레이팅 펩티드, 고정된 금속상에서 정제를 가능하게 하는, 히스티딘-트립토판 모듈, 글루타티온에 결합하는 서열(예컨대, GST), 헤마글루티닌(HA) 태그(인플루엔자 헤마글루티닌 단백질로부터 유래된 에피토프에 상응; 예컨대, 문헌 [Wilson et al., Cell 37:767 [1984] 참조), 말토스 결합 단백질 서열, FLAGS 확장/친화성 정제 시스템(예컨대, Immunex Corp로부터 입수가능한 시스템)에서 이용되는 FLAG 에피토프 등을 포함하나, 이에 제한되지 않는다. 본원에 기술된 조성물 및 방법에서의 사용이 고려되는 한 발현 벡터는 엔테로키나제 절단 부위에 의해 이격된 폴리히스티딘 영역에 융합된 본 발명의 폴리펩티드를 포함하는 융합 단백질의 발현을 제공한다. 히스티딘 잔기는 IMIAC(고정된 금속 이온 친화성 크로마토그래피: immobilized metal ion affinity chromatography; 예컨대, 문헌 [Porath et al., Prot. Exp. Purif., 3:263-281 [1992]] 참조) 상에서 정제를 용이하게 하는 한편, 엔테로키나제 절단 부위는 융합 단백질로부터 변이체 PGA 폴리펩티드를 분리시키기 위한 수단을 제공한다. pGEX 벡터(Promega)는 글루타티온 S-트랜스퍼라제(GST: glutathione S-transferase)와의 융합 단백질로서 외래 폴리펩티드를 발현시키는데 사용될 수 있다. 일반적으로, 상기 융합 단백질은 가용성이고, 리간드-아가로스 비드(예컨대, GST-융합체의 경우 글루타티온-아가로스)에 흡착 후, 유리 리간드 존재에서 용출시킴으로써 용해된 세포로부터 쉽게 정제될 수 있다.

실험부

본 개시내용의 다양한 특징 및 실시양태가 하기의 대표적인 실시예에서 예시되지만, 이는 예시를 위한 것이고, 제한하는 것이 아니다.

하기 실험 개시내용에서, 하기 약어가 적용된다: ppm(백만분율: parts per million); M(몰 농도); mM(밀리몰 농도), uM 및 μM(마이크로몰 농도); nM(나노몰 농도); mol(몰수); gm 및 g(그램); mg(밀리그램); ug 및 ㎍(마이크로그램); L 및 ℓ(리터); ml 및 mL(밀리리터); cm(센티미터); mm(밀리미터); um 및 ㎛(마이크로미터); sec(초); min(s)(분); h 및 hr(시간); U(유니트); MW(분자량: molecular weight); rpm(분당 회전수: rotations per minute); ℃(섭씨 온도); RT(실온: room temperature); CDS(코딩 서열: coding sequence); DNA(데옥시리보핵산); RNA(리보핵산); TB(테리픽 브로쓰(Terrific Broth); 12 g/L 박토-트립톤, 24 g/L 효모 추출물, 4 mL/L 글리세롤, 65 mM 인산칼륨, pH 7.0, 1 mM MgSO4); CAM(클로람페니콜); PMBS(폴리믹신 B 술페이트: polymyxin B sulfate); IPTG(이소프로필 티오갈락토시드: isopropyl thiogalactoside); TFA(트리플루오로아세트산); HPLC(고성능 액상 크로마토그래피); FIOPC(양성 대조군 대비 개선 배수: fold improvement over positive control); HTP(고처리량: high throughput); LB(루리아 브로쓰: Luria broth); 코데시스(Codexis)(Codexis, Inc.: 미국 캘리포니아주 레드우드 시티 소재); 시그마-알츠리치(Sigma-Aldrich)(Sigma-Aldrich: 미국 미주리주 세인트 루이스 소재); 밀리포어(Millipore)(Millipore, Corp.: 미국 매사추세츠주 빌러리카 소재); 디프코(Difco)(Difco Laboratories, BD Diagnostic Systems: 미국 미시간주 디트로이트 소재); 다이셀(Daicel)(Daicel: 미국 펜실베니아주 웨스트체스터 소재); 제네티스(Genetix)(Genetix USA, Inc.: 미국 오리건주 비버턴 소재); 몰레큘라 디바이시스(Molecular Devices)(Molecular Devices, LLC: 미국 캘리포니아주 서니배일 소재); 어플라이드 바이오시스템즈(Applied Biosystems)(Applied Biosystems, Life Technologies, Corp의 지부: 미국 뉴욕주 그랜드 아일랜드 소재), 애질런트(Agilent)(Agilent Technologies, Inc.: 미국 캘리포니아주 산타클라라 소재); 써모 사이언티픽(Thermo Scientific)(Thermo Fisher Scientific의 지부: 미국 매사추세츠주 월섬 소재); 인폴스(Infors)(Infors-HT: 스위스 보트민겐/바젤 소재); 코닝(Corning)(Corning, Inc.: 미국 캘리포니아주 팔로 알토 소재); 및 바이오-래드(Bio-Rad Laboratories: 미국 캘리포니아주 허큘리스 소재); 마이크로플루이딕스(Microfluidics)(Microfluidics Corp.: 미국 매사추세츠주 뉴턴 소재).

실시예 1

재조합 PGA 유전자를 함유하는 이. 콜라이 발현 숙주

본 발명의 변이체를 제조하는 데 사용된 초기 PGA 효소는 코덱스^® 아실라제 패널(Codex^® Acylase Panel)(Codexis), 또는 공동 소유의 미국 가특허 출원 제62/158,118호에 개시된 변이체로부터 수득되었다. PGA 패널 플레이트는 야생형 클루이베라 시트로필라 PGA와 비교하여, 개선된 특성을 가지는 조작된 PGA 폴리펩티드의 콜렉션을 포함한다. 야생형 PGA 유전자는 54 aa 스페이서 영역에 의해 연결된 알파 서브유니트(23.8 KDa) 및 베타 서브유니트(62.2 KDa)로 구성된 이종이량체이다. 스페이서 영역의 존재에 기인하여, 활성 단백질 형성을 위해 자가 프로세싱 단계가 요구된다. 야생형 유전자는 스페이서 영역을 제거하도록 변형됨에 따라 자가 프로세싱 단계가 없어졌다. 코덱스^® 아실라제 패널(Codexis)은 스페이서 영역이 결여된 PGA 변이체를 함유한다(예컨대, 미국 특허 출원 공개 2010/0143968 A1 참조). PGA-코딩 유전자는 lacI 리프레서의 제어하에 있는 lac 프로모터에 작동적으로 연결된 발현 벡터 pCK110900에 클로닝되었다(예컨대, 미국 특허 출원 공개 번호 2006/0195947의 도 3 참조). 발현 벡터는 또한 P15a 복제 기점 및 클로람페니콜 내성 유전자를 함유한다. 당업계에 공지된 표준 방법을 사용하여, 생성된 플라스미드를 이. 콜라이 W3110에 형질전환시켰다. 당업계에 공지된 바와 같이(예컨대, 미국 특허 번호 8,383,346 및 WO2010/144103 참조), 세포에 대해 클로람페니콜 선별을 수행하여 형질전환체를 단리시켰다.

실시예 2

HTP PGA 함유 습윤 세포 펠릿 제조

단일클론 콜로니로부터의 재조합 PGA 코딩 융전자를 함유하는 이. 콜라이 세포를 96 웰 얕은 웰 마이크로타이터 플레이트의 웰 중 1% 글루코스 및 30 ㎍/mL 클로람페니콜을 함유하는 180 ㎕ LB에 접종하였다. 플레이트를 O₂ 투과성 씰(permeable seal)로 밀봉하고, 배양물을 30℃, 200 rpm 및 85% 습도에서 밤새도록 성장시켰다. 이어서, 10 ㎕의 각 세포 배양물을 390 mL TB 및 30 ㎍/mL CAM을 함유하는 96 웰 깊은 웰 플레이트의 웰로 옮겼다. 깊은 웰 플레이트를 O₂ 투과성 씰로 밀봉하고, OD600 0.6-0.8에 도달할 때까지 30℃, 250 rpm 및 85% 습도에서 인큐베이션시켰다. 이어서, 세포 배양물을 1 mM의 최종 농도로 IPTG에 의해 유도시키고, 원래 사용된 것과 동일한 조건하에서 밤새도록 인큐베이션시켰다. 이어서, 세포를 4,000 rpm으로 10 min 동안 원심분리를 사용하여 펠릿화시켰다. 상청액을 폐기하고, 펠릿은 용해 전에 -80℃에서 냉동시켰다.

실시예 3

HTP PGA 함유 세포 용해물 제조

먼저, 실시예 2에 기술된 바와 같이 제조된 각 웰 중의 세포 페이스트에 10 mM 트리스-HCl 완충제, pH 7.5, 1 mg/mL 리소자임, 및 0.5 mg/mL PMBS를 함유하는 200 ㎕의 용해 완충제를 첨가하였다. 세포를 실온에서 2시간 동안 벤치 탑 진탕기 상에서 진탕시키면서 용해시켰다. 이어서, 플레이트를 4℃에서 15 min 동안 4,000 rpm으로 원심분리하였다. 투명한 상청액을 그의 활성 수준을 측정하기 위해 생물촉매 반응에 사용하였다.

실시예 4

진탕 플라스크( SF : Shake Flask) 배양물로부터의 동결건조된 용해물 제조

상기에 기술된 바와 같이 성장시킨 선택된 HTP 배양물을 1% 글루코스 및 30 ㎍/ml CAM을 포함하는 LB 아가 플레이트 상에 플레이팅하고, 37℃에서 밤새도록 성장시켰다. 각 배양물로부터의 단일 콜로니를 1% 글루코스 및 30㎍/ml CAM을 포함하는 6 ml의 LB로 옮겼다. 배양물을 30℃에서 18 h 동안 250 rpm으로 성장시켰고, 30 ㎍/ml CAM을 함유하는 250 ml의 TB에 대략 1:50으로, 최종 OD₆₀₀이 0.05가 될 때까지 계대배양하였다. 배양물을 OD₆₀₀이 0.6-0.8이 될 때까지, 대략 195분 동안 30℃, 250 rpm으로 성장시키고, 1 mM IPTG로 유도시켰다. 이어서, 배양물을 20 h 동안 30℃, 250 rpm으로 성장시켰다. 배양물을 4,000 rpm x 20 min 동안 원심분리시켰다. 상청액을 폐기하고, 펠릿을 30 ml의 20 mM TRIS-HCl, pH 7.5 중에 재현탁시켰다. 세포를 펠릿화(4,000 rpm x 20 min)시키고, -80℃에서 120분 동안 냉동시켰다. 냉동된 펠릿을 30 ml의 20 mM TRIS-HCl pH 7.5 중에 재현탁시키고, 마이크로플루이다이저(Microfluidizer) 시스템(Microfluidics)을 사용하여 18,000 psi에서 용해시켰다. 용해물을 펠릿화(10,000 rpm x 60 min)시키고, 상청액을 냉동 및 동결건조시켜 진탕 플라스크(SF) 효소를 생성하였다.

실시예 5

B29 위치에서의 삼중 보호된 인슐린의 탈아실화에서

서열 번호: 8 대비 개선

B29 탈아실화된 생성물의 제조를 위해 미국 가특허 출원 제62/158,118호에 개시된 변이체에 대해 스크리닝한 후, 서열 번호: 8을 모체 효소로서 선택하였다. 잘 확립된 기술(예컨대, 포화 돌연변이유발법, 앞서 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자로 이루어진 라이브러리를 제조하였다. 실시예 2에 기술된 바와 같이 HTP에서 각 유전자에 의해 코딩되는 폴리펩티드를 제조하고, 실시예 3에 기술된 바와 같이 가용성 용해물을 생성하였다. 5 g/L A1/B1/B29 트리-페닐 아세테이트 인슐린, 200 mM 트리스 완충제 pH=8.3 및 40 ㎕ 조 용해물로 구성된 200 ㎕ 반응물 중에서 각 변이체를 30℃에서 3시간 동안 스크리닝하였다. 96 웰 플레이트를 가열 밀봉하고, 써모트론(Thermotron)^® 진탕기 중 100 rpm으로 인큐베이션시켰다. 반응물을 200 ㎕ 아세토니트릴 또는 디메틸아세트아미드로 켄칭하고, 벤치 탑 진탕기를 사용하여 5분 동안 혼합하였다. 이어서, 플레이트를 4,000 rpm으로 5분 동안 원심분리하고, 물로 2배 희석시키고, 분석을 위해 HPLC에 적재하였다.

서열 번호: 8 대비 활성(활성 FIOP)은 서열 번호: 8에 의해 산출된 전환율(%) 대비 변이체에 의해 형성된 생성물의 전환율(%)로서 계산되었고, 이는 표 5.1에 제시되어 있다. 전환율(%)은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 기질, 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다.

실시예 6

B29 위치에서의 삼중 보호된 인슐린의 탈아실화에서

서열 번호: 14 대비 개선

실시예 5에 기술된 변이체에 대해 스크리닝한 후, 서열 번호: 14를 모체 효소로서 선택하였다. 잘 확립된 기술(예컨대, 포화 돌연변이유발법, 앞서 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자로 이루어진 라이브러리를 제조하였다. 실시예 2에 기술된 바와 같이 HTP에서 각 유전자에 의해 코딩되는 폴리펩티드를 제조하고, 실시예 3에 기술된 바와 같이 가용성 용해물을 생성하였다.

10 g/L A1/B1/B29 트리-페닐 아세테이트 인슐린, 200 mM 트리스 완충제 pH=8.3 및 20 ㎕ 조 용해물로 구성된 200 ㎕ 반응물 중에서 각 변이체를 30℃에서 24시간 동안 스크리닝하였다. 96 웰 플레이트를 가열 밀봉하고, 써모트론 진탕기 중 100 rpm으로 인큐베이션시켰다. 반응물을 200 ㎕ 아세토니트릴 또는 디메틸아세트아미드로 켄칭하고, 벤치 탑 진탕기를 사용하여 5분 동안 혼합하였다. 이어서, 플레이트를 4,000 rpm으로 5분 동안 원심분리하고, 물로 2배 희석시키고, 분석을 위해 HPLC에 적재하였다.

서열 번호: 14 대비 활성(활성 FIOP)은 서열 번호: 14에 의해 산출된 전환율(%) 대비 변이체에 의해 형성된 생성물의 전환율(%)로서 계산되었고, 이는 표 6.1에 제시되어 있다. 전환율(%)은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 기질, 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다.

실시예 7

B29 위치에서의 삼중 보호된 인슐린의 탈아실화에서

서열 번호: 300 대비 개선

실시예 6에 기술된 변이체에 대해 스크리닝한 후, 서열 번호: 300을 모체 효소로서 선택하였다. 잘 확립된 기술(예컨대, 포화 돌연변이유발법, 앞서 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자로 이루어진 라이브러리를 제조하였다. 실시예 2에 기술된 바와 같이 HTP에서 각 유전자에 의해 코딩되는 폴리펩티드를 제조하고, 실시예 3에 기술된 바와 같이 가용성 용해물을 생성하였다.

50 g/L A1/B1/B29 트리-페닐 아세테이트 인슐린, 200 mM 트리스 완충제 pH=8.3 및 2.5 ㎕ 조 용해물로 구성된 200 ㎕ 반응물 중에서 각 변이체를 30℃에서 24시간 동안 스크리닝하였다. 96 웰 플레이트를 가열 밀봉하고, 써모트론 진탕기 중 100 rpm에서 인큐베이션시켰다. 반응물을 200 ㎕ 아세토니트릴 또는 디메틸아세트아미드로 켄칭하고, 벤치 탑 진탕기를 사용하여 5분 동안 혼합하였다. 이어서, 플레이트를 4,000 rpm으로 5분 동안 원심분리하고, 물로 2배 희석시키고, 분석을 위해 HPLC에 적재하였다.

서열 번호: 300 대비 활성(활성 FIOP)은 서열 번호: 300에 의해 산출된 전환율(%) 대비 변이체에 의해 형성된 생성물의 전환율(%)로서 계산되었고, 이는 표 7.1에 제시되어 있다. 전환율(%)은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 기질, 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다.

실시예 8

A1 위치에서의 삼중 보호된 인슐린의 탈아실화에서

서열 번호: 1262 또는 1288 대비 개선

코덱스^® 아실라제 패널(Codexis)로부터의 변이체, 및 미국 가특허 출원 제62/158,118호에 개시된 변이체에 대해 스크리닝한 결과, 위치 A1에서 A1/B1/B29 트리-페닐 아세테이트 인슐린으로부터 보호기를 제거하는 데 있어서 변이체 서열 번호: 1262 및 1288이 가장 우수한 효소인 것으로 확인되었다. 위치 A1에서의 삼중 보호된 인슐린의 탈아실화에 대한 개선된 활성 및 화학선택성을 표적화하는 라이브러리에 대한 모체 효소로서 서열 번호: 1262를 포함하는 변이체를 선택하였다. 잘 확립된 기술(예컨대, 포화 돌연변이유발법, 앞서 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자로 이루어진 라이브러리를 제조하였다. 실시예 2에 기술된 바와 같이 HTP에서 각 유전자에 의해 코딩되는 폴리펩티드를 제조하고, 실시예 3에 기술된 바와 같이 가용성 용해물을 생성하였다. 5 g/L A1/B1/B29 트리-페닐 아세테이트 인슐린, 0.1 M 트리스-HCl 완충제 pH=8.0, 17 g/L 메틸 페닐아세테이트 및 80 ㎕ 가용성 용해물로 구성된 200 ㎕ 반응물 중에서 각 변이체를 30℃에서 5시간 동안 스크리닝하였다. 96 웰 플레이트를 가열 밀봉하고, 써모트론^® 진탕기 중 100 rpm으로 인큐베이션시켰다. 반응물을 300 ㎕ 아세토니트릴로 켄칭하고, 벤치 탑 진탕기를 사용하여 5분 동안 혼합하였다. 이어서, 플레이트를 4,000 rpm으로 5분 동안 원심분리하고, 분석을 위해 HPLC에 적재하였다.

서열 번호: 1262 또는 1288 대비 전환율(%)(전환율(%) FIOP)은 서열 번호: 1262 또는 1288에 의해 산출된 전환율(%) 대비 변이체에 의해 형성된 생성물의 전환율(%)로서 계산되었고, 이는 표 8.1 및 8.2에 제시되어 있다. 전환율(%)은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 기질, 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다.

서열 번호: 1262 또는 1288 대비 선택성(%)(선택성(%) FIOP)은 서열 번호: 1262 또는 1288에 의해 산출된 선택성(%) 대비 변이체에 의해 형성된 생성물의 선택성(%)으로서 계산되었고, 이는 표 8.1 및 8.2에 제시되어 있다. 선택성(%)은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다.

실시예 9

A1 위치에서의 삼중 보호된 인슐린의 탈아실화에서

서열 번호: 1036 대비 개선

실시예 8에 기술된 변이체에 대해 스크리닝한 후, 서열 번호: 1036을 모체 효소로서 선택하였다. 잘 확립된 기술(예컨대, 포화 돌연변이유발법, 앞서 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자로 이루어진 라이브러리를 제조하였다. 실시예 2에 기술된 바와 같이 HTP에서 각 유전자에 의해 코딩되는 폴리펩티드를 제조하고, 실시예 3에 기술된 바와 같이 가용성 용해물을 생성하였다.

5 g/L A1/B1/B29 트리-페닐 아세테이트 인슐린, 200 mM 트리스 완충제 pH=8.0 및 10 ㎕ 가용성 용해물로 구성된 200 ㎕ 반응물 중에서 각 변이체를 30℃에서 5시간 동안 스크리닝하였다. 96 웰 플레이트를 가열 밀봉하고, 써모트론^® 진탕기 중 100 rpm으로 인큐베이션시켰다. 반응물을 200 ㎕ 아세토니트릴 또는 디메틸아세트아미드로 켄칭하고, 벤치 탑 진탕기를 사용하여 5분 동안 혼합하였다. 이어서, 플레이트를 4,000 rpm으로 5분 동안 원심분리하고, 물로 2배 희석시키고, 분석을 위해 HPLC에 적재하였다.

서열 번호: 1036 대비 전환율(%)(전환율(%) FIOP)은 서열 번호: 1036에 의해 산출된 전환율(%) 대비 변이체에 의해 형성된 생성물의 전환율(%)로서 계산되었고, 이는 표 9.1에 제시되어 있다. 전환율(%)은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 기질, 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다.

서열 번호: 1036 대비 선택성(%)(선택성(%) FIOP)은 서열 번호: 1036에 의해 산출된 선택성(%) 대비 변이체에 의해 형성된 생성물의 선택성(%)으로서 계산되었고, 이는 표 9.1에 제시되어 있다. 선택성(%)은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다.

실시예 10

A1 위치에서의 삼중 보호된 인슐린의 탈아실화에서

서열 번호: 1194 대비 개선

실시예 9에 기술된 변이체에 대해 스크리닝한 후, 서열 번호: 1194를 모체 효소로서 선택하였다. 잘 확립된 기술(예컨대, 포화 돌연변이유발법, 앞서 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자로 이루어진 라이브러리를 제조하였다. 실시예 2에 기술된 바와 같이 HTP에서 각 유전자에 의해 코딩되는 폴리펩티드를 제조하고, 실시예 3에 기술된 바와 같이 가용성 용해물을 생성하였다.

15 g/L A1/B1/B29 트리-페닐 아세테이트 인슐린, 200 mM 트리스 완충제 pH=8.0 및 10 ㎕ 가용성 용해물로 구성된 200 ㎕ 반응물 중에서 각 변이체를 30℃에서 5시간 동안 스크리닝하였다. 96 웰 플레이트를 가열 밀봉하고, 써모트론 중 100 rpm으로 인큐베이션시켰다. 반응물을 200 ㎕ 아세토니트릴 또는 디메틸아세트아미드로 켄칭하고, 벤치 탑 진탕기를 사용하여 5분 동안 혼합하였다. 이어서, 플레이트를 4,000 rpm으로 5분 동안 원심분리하고, 물로 2배 희석시키고, 분석을 위해 HPLC에 적재하였다.

서열 번호: 1194 대비 전환율(%)(전환율(%) FIOP)은 서열 번호: 1194에 의해 산출된 전환율(%) 대비 변이체에 의해 형성된 생성물의 전환율(%)로서 계산되었고, 이는 표 10.1에 제시되어 있다. 전환율(%)은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 기질, 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다.

서열 번호: 1194 대비 선택성(%)(선택성(%) FIOP)은 서열 번호: 1194에 의해 산출된 선택성(%) 대비 변이체에 의해 형성된 생성물의 선택성(%)으로서 계산되었고, 이는 표 10.1에 제시되어 있다. 선택성(%)은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다.

실시예 11

A1, B1 및 B29 위치에서의 인슐린의 아실화에서의 개선

코덱스^® 아실라제 패널(Codexis) 중의, 및 미국 가특허 출원 제62/158,118호에 개시된 변이체로 이루어진 선택된 변이체들을 A1, B1, 및 B29 위치에서 인술린을 아실화시킬 수 있는 그의 능력에 대해 스크리닝하였다. 실시예 2에 기술된 바와 같이 HTP에서 각 유전자에 의해 코딩되는 폴리펩티드를 제조하고, 실시예 3에 기술된 바와 같이 가용성 용해물을 생성하였다.

10 g/L 인슐린, 0.1 M CHES 완충제 pH=10, 17 g/L 메틸 페닐아세테이트 및 20 ㎕ 가용성 용해물로 구성된 200 ㎕ 반응물 중에서 각 변이체를 30℃에서 20시간 동안 스크리닝하였다. 96 웰 플레이트를 가열 밀봉하고, 써모트론^® 진탕기 중 300 rpm으로 인큐베이션시켰다. 반응물을 200 ㎕ 아세토니트릴로 켄칭하고, 벤치 탑 진탕기를 사용하여 5분 동안 혼합하였다. 이어서, 플레이트를 4,000 rpm으로 5분 동안 원심분리하고, 분석을 위해 HPLC에 적재하였다.

전환율(%)(전환율(%))은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 기질, 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다. 선택성(%)(선택성(%))은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다. 결과는 도 2a 내지 도 2h에 제시되어 있다.

실시예 12

A1, B1 및 B29 위치에서의 인슐린의 아실화에서

서열 번호: 1288 대비 개선

실시예 11에 기술된 변이체에 대해 스크리닝하고, 위치 B29에서 인슐린을 아실화시키는 데 있어서 가장 우수한 효소인 것으로 확인한 후, 서열 번호: 1288을 모체 효소로서 선택하였다. 잘 확립된 기술(예컨대, 포화 돌연변이유발법, 앞서 확인된 유익한 돌연변이의 재조합)을 사용하여 조작된 유전자로 이루어진 라이브러리를 제조하였다. 실시예 2에 기술된 바와 같이 HTP에서 각 유전자에 의해 코딩되는 폴리펩티드를 제조하고, 실시예 3에 기술된 바와 같이 가용성 용해물을 생성하였다.

10 g/L 인슐린, 0.1 M TRIS 완충제 pH=9.25, 20% 아세토니트릴, 17 g/L 메틸 페닐아세테이트 및 10 ㎕ 정화된 용해물로 구성된 200 ㎕ 반응물 중에서 각 변이체를 30℃에서 5시간 동안 스크리닝하였다. 96 웰 플레이트를 가열 밀봉하고, 써모트론® 진탕기 중 100 rpm으로 인큐베이션시켰다. 반응물을 200 ㎕ 아세토니트릴로 켄칭하고, 벤치 탑 진탕기를 사용하여 5분 동안 혼합하였다. 이어서, 플레이트를 4,000 rpm으로 5분 동안 원심분리하고, 분석을 위해 HPLC에 적재하였다.

서열 번호: 1288 대비 전환율(%)(전환율(%) FIOP)은 서열 번호: 1288에 의해 산출된 전환율(%) 대비 변이체에 의해 형성된 생성물의 전환율(%)로서 계산되었고, 이는 표 12.1, 12, 2, 12,3, 12.4, 12.5, 12.6, 및 12.7에 제시되어 있다. 전환율(%)은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 기질, 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다.

서열 번호: 1288 대비 선택성(%)(선택성(%) FIOP)은 서열 번호: 1288에 의해 산출된 선택성(%) 대비 변이체에 의해 형성된 생성물의 선택성(%)으로서 계산되었고, 이는 표 12.1, 12, 2, 12,3, 12.4, 12.5, 12.6, 및 12.7에 제시되어 있다. 선택성(%)은 HPLC 분석에 의해 관찰된 바와 같은, 생성물 피크 면적을 생성물, 및 불순물/부산물 피크 면적의 총합으로 나눔으로써 계산되었다.

실시예 13

대체 아실 공여자를 이용한 인슐린의 아실화

메틸 페닐아세테이트에 대한 대체물로서 (메틸 2-(4-하이드록시페닐)아세테이트 또는 2-(4-하이드록시페닐)아세트아미드)를 사용하여 표 13에 열거된 5개의 변이체의 아실화를 평가하였다. 실시예 4에 기술된 바와 같이 진탕 플라스크 분말을 제조하였다. 각각 0.1 M CHES, pH 10, 5% 아세토니트릴, 15 g/L 인슐린, 26 g/L 아실 공여자(메틸 2-(4-하이드록시페닐)아세테이트 또는 2-(4-하이드록시페닐)아세트아미드) 및 1 g/L 동결건조된 효소 분말을 함유하는 96 웰 깊은 웰 플레이트 주에서 반응을 수행하였다. HTP 플레이트를 가열 밀봉하고, 써모트론^® 진탕기(3 mm 쓰로우(throw), 모델 #AJ185, Infors) 중 30℃에서 300 rpm으로 2시간 동안 인큐베이션시켰다. 반응물을 200 ㎕ 아세토니트릴로 켄칭하고, 벤치 탑 진탕기를 사용하여 5분 동안 혼합하였다. 이어서, 플레이트를 4,000 rpm으로 5분 동안 원심분리하고, 분석을 위해 HPLC에 적재하였다.

각 변이체의 활성은 HPLC 분석에 의해 측정된 바와 같은, 모든 생성물의 피크 면적을 기질 및 다양한 인슐리 생성물의 면적의 총합으로 나눔으로써 정량화된 전환율(%)로서 계산된다.

실시예 14

인슐린 및 그의 아실화된 생성물의 분석적 검출

표 14.1, 14.2, 14.3, 14.4, 및 14.5의 분석 방법을 사용하여 실시예 5-12에 기술된 데이터를 수집하였다. 본원에 제공된 방법들은 모두 본 발명을 사용하여 제조된 변이체를 분석하는 데 사용된다. 도 1에 제시된 결과는 본 방법에 대한 화합물의 용출 순서에 상응하다.

본 출원에서 인용된 모든 공개문헌, 특허, 특허 출원 및 다른 문헌들은 각각의 개별 공개문헌, 특허, 특허 출원 또는 다른 문헌이 모든 목적을 위해 참조로 포함되는 것으로 개별적으로 명시된 것과 같은 정도로 모든 목적을 위해 그 전문이 본원에서 참조로 포함된다.

다양한 구체적인 실시양태가 예시되고 기술되었지만, 본 발명(들)의 정신 및 범주로부터 벗어남 없이, 다양하게 변형될 수 있다는 것을 이해할 것이다.

SEQUENCE LISTING <110> CODEXIS, INC. <120> PENICILLIN-G ACYLASES <130> CX2-154WO1 <140> To Be Assigned <141> 2017-05-05 <150> US 62/332,103 <151> 2016-05-05 <160> 1902 <170> PatentIn version 3.5 <210> 1 <211> 2541 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates from K. citrophila <400> 1 atgaaaaata gaaatcgtat gatcgtgaac ggtattgtga cttccctgat ctgttgttct 60 agcctgtcag cgctggcggc aagcccgcca accgaggtta agatcgttcg cgatgaatac 120 ggcatgccgc atatttacgc cgatgatacc tatcgactgt tttacggcta tggctacgtg 180 gtggcgcagg atcgcctgtt ccagatggaa atggcgcgcc gcagtactca ggggaccgtc 240 tccgaggtgc tgggcaaagc attcgtcagt tttgataaag atattcgcca gaactactgg 300 ccggattcta ttcgcgcgca gatagcttcc ctctccgctg aggataaatc cattctgcag 360 ggctatgccg atggcatgaa tgcgtggatc gataaagtga acgccagccc cgataagctg 420 ttaccccagc agttctccac ctttggtttt aaacccaagc attgggaacc gtttgatgtg 480 gcgatgattt ttgtcggcac catggcgaac cggttttctg acagcaccag cgaaattgat 540 aacctggcgc tgctgacggc gctaaaagat aaatacggca agcagcaggg catggcggtc 600 tttaaccagc tgaaatggct ggttaatcct tccgcgccaa ccaccattgc ggcgcgggaa 660 agcgcctatc cgctgaagtt tgatctgcaa aacacgcaaa cggcggcgct gctgccgcgc 720 tacgaccagc cggcaccgat gctcgaccgc ccggcaaaag ggaccgatgg cgcgctgctg 780 gcgctgaccg ccgatcagaa ccgggaaact atcgccgcgc agttcgcgca aagcggcgct 840 aacggcctgg ctggctaccc gaccactagc aatatgtggg tgattggcaa aaacaaagcc 900 caggatgcga aggccattat ggtcaatggg ccgcagtttg gttggtatgc gccggcgtac 960 acctacggta tcggcctgca cggcgcgggc tatgacgtca ccggcaatac gccgtttgcc 1020 tatccgggcc tcgtttttgg tcacaacggc accatttcat ggggatccac cgccggtttt 1080 ggtgatgatg tcgatatctt tgccgaaaaa ctttccgccg agaagccggg ctattaccag 1140 cataacggcg agtgggtgaa gatgttgagc cgcaaggaga ctattgcggt caaagacggc 1200 cagccggaga cctttaccgt ttggcgcacg ctgcacggca acgtcattaa aaccgatact 1260 gcgacgcaga ccgcctatgc caaagcgcgc gcctgggatg gcaaagaggt ggcgtccctg 1320 ctggcgtgga cgcaccagat gaaggccaaa aactggccgg agtggacgca gcaggcggcc 1380 aaacaggcgc tgaccattaa ctggtactac gccgatgtga acggcaatat cggctatgtg 1440 cataccggcg cctatccgga tcgccagccc ggccacgacc cgcgtttgcc ggttcccggc 1500 actggaaaat gggactggaa agggttgctg tcgtttgatt tgaatccgaa agtgtataac 1560 ccgcagtcgg gctatatcgc caactggaac aactcgccgc aaaaagacta cccggcctct 1620 gatctgttcg cgttcctgtg gggcggtgcg gatcgagtta ctgagatcga cacgatcctc 1680 gataagcaac cgcgcttcac cgccgatcag gcgtgggatg tgatccgcca aaccagccgt 1740 cgggatctca acctgcggtt gttcttaccg gcgctgaagg acgccaccgc gaacctggcg 1800 gaaaacgatc cgcgccgcca actggtggat aaactggcga gctgggacgg tgaaaacctt 1860 gtcaacgatg acggaaaaac ctatcagcaa ccgggatcgg cgattcttaa cgcctggctg 1920 accagcatgc tcaagcgcac ggtggttgcc gcggtcccag cgccgtttgg caagtggtac 1980 agcgccagtg gctatgaaac cacccaggac gggccaaccg gctcgctgaa catcagcgtg 2040 ggggcgaaaa tcctctacga agctctgcag ggtgataagt cgccaatccc gcaggcggtc 2100 gatctgtttg gcgggaaacc gcagcaggaa gtgatactgg cggcgctgga cgacgcttgg 2160 cagacgctgt caaaacgcta cggtaacgac gtcaccggct ggaaaacccc tgccatggcg 2220 cttaccttcc gggccaataa cttcttcggc gtgccgcagg cggcagcaaa agaggcgcgt 2280 catcaggcgg agtaccagaa ccgcggtacg gaaaacgaca tgattgtctt ctcaccgacg 2340 tcgggtaacc gcccggttct tgcctgggat gtggtggcgc cggggcaaag cggttttatc 2400 gcgccggatg gcaaagccga taagcactat gacgatcagc tgaaaatgta cgagagcttt 2460 ggccgtaaat cgctgtggtt aacgcctcag gacgttgacg agcacaaaga gtctcaggaa 2520 gtgctgcagg tacagcgcta a 2541 <210> 2 <211> 846 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 2 Met Lys Asn Arg Asn Arg Met Ile Val Asn Gly Ile Val Thr Ser Leu 1 5 10 15 Ile Cys Cys Ser Ser Leu Ser Ala Leu Ala Ala Ser Pro Pro Thr Glu 20 25 30 Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala Asp 35 40 45 Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln Asp 50 55 60 Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr Val 65 70 75 80 Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile Arg 85 90 95 Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu Ser 100 105 110 Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn Ala 115 120 125 Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln Gln 130 135 140 Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp Val 145 150 155 160 Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser Thr 165 170 175 Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys Tyr 180 185 190 Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu Val 195 200 205 Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr Pro 210 215 220 Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala Ala Leu Leu Pro Arg 225 230 235 240 Tyr Asp Gln Pro Ala Pro Met Leu Asp Arg Pro Ala Lys Gly Thr Asp 245 250 255 Gly Ala Leu Leu Ala Leu Thr Ala Asp Gln Asn Arg Glu Thr Ile Ala 260 265 270 Ala Gln Phe Ala Gln Ser Gly Ala Asn Gly Leu Ala Gly Tyr Pro Thr 275 280 285 Thr Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys 290 295 300 Ala Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Ala Pro Ala Tyr 305 310 315 320 Thr Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn 325 330 335 Thr Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile 340 345 350 Ser Trp Gly Ser Thr Ala Gly Phe Gly Asp Asp Val Asp Ile Phe Ala 355 360 365 Glu Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu 370 375 380 Trp Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly 385 390 395 400 Gln Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile 405 410 415 Lys Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp 420 425 430 Asp Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys 435 440 445 Ala Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu 450 455 460 Thr Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val 465 470 475 480 His Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu 485 490 495 Pro Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe 500 505 510 Asp Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn 515 520 525 Trp Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala 530 535 540 Phe Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu 545 550 555 560 Asp Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg 565 570 575 Gln Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu 580 585 590 Lys Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu 595 600 605 Val Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp 610 615 620 Gly Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu 625 630 635 640 Thr Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe 645 650 655 Gly Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro 660 665 670 Thr Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala 675 680 685 Leu Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly 690 695 700 Gly Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp 705 710 715 720 Gln Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr 725 730 735 Pro Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro 740 745 750 Gln Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg 755 760 765 Gly Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg 770 775 780 Pro Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile 785 790 795 800 Ala Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met 805 810 815 Tyr Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val 820 825 830 Asp Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Arg 835 840 845 <210> 3 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 3 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 4 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 4 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 5 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 5 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta taatccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcctttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gccggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat caactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag cgactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgccaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgttaagttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttttctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 6 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 6 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Asn Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Leu Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Ala Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Ala Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Lys Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 7 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 7 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 8 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 8 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 9 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 9 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gatgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttttctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 10 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 10 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 11 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 11 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgtgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat caactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gatgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt tcggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccct ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 12 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 12 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Phe Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Leu Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 13 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 13 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 14 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 14 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 15 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 15 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca ataccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 16 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 16 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Asn 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 17 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 17 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgaa aaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 18 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 18 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Lys Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 19 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 19 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 20 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 20 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 21 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 21 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaacaaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 22 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 22 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Gln Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 23 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 23 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 24 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 24 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 25 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 25 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagcaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 26 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 26 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Ala Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 27 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 27 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctga atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 28 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 28 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 29 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 29 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggcggtct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 30 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 30 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Gly Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 31 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 31 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 32 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 32 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 33 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 33 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca caggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 34 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 34 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 35 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 35 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgcg taacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 36 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 36 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Arg Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 37 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 37 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatattt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 38 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 38 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 39 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 39 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattatt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 40 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 40 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 41 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 41 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac agtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 42 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 42 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 43 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 43 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 44 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 44 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 45 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 45 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggcgcact gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 46 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 46 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ala Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 47 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 47 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcatg gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 48 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 48 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Trp Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 49 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 49 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccaccga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 50 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 50 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Thr Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 51 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 51 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctga aagcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 52 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 52 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Lys Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 53 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 53 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaaaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 54 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 54 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Lys Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 55 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 55 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg taccttgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 56 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 56 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Leu Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 57 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 57 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggaa cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 58 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 58 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Glu Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 59 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 59 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcatt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 60 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 60 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 61 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 61 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccgc aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 62 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 62 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Ala Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 63 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 63 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagagtgt cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 64 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 64 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Cys Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 65 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 65 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccca aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 66 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 66 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 67 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 67 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacctat 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccct taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 68 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 68 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Tyr Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Leu Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 69 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 69 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggccatct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 70 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 70 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly His Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 71 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 71 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcaaaa gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 72 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 72 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Lys Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 73 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 73 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcatgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 74 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 74 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 75 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 75 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 76 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 76 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 77 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 77 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga acttgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 78 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 78 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Leu Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 79 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 79 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgagtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 80 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 80 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Ser 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 81 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 81 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagt gtgagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 82 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 82 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Cys Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 83 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 83 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccatg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 84 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 84 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Met Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 85 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 85 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 caagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 86 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 86 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Gln Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 87 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 87 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 88 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 88 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 89 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 89 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 90 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 90 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 91 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 91 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gatgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagcat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 caagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 92 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 92 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Asp Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Gln Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 93 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 93 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 94 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 94 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 95 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 95 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagcat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 96 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 96 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 97 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 97 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagcat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctacaaaac 2280 acgcaaacgg cgtaa 2295 <210> 98 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 98 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 99 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 99 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 100 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 100 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 101 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 101 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagcat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 102 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 102 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 103 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 103 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 104 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 104 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 105 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 105 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 106 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 106 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 107 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 107 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gatgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 caagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 108 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 108 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Asp Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Gln Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 109 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 109 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacaggatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 110 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 110 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Arg Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 111 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 111 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 112 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 112 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 113 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 113 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 114 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 114 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 115 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 115 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 116 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 116 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 117 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 117 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagcat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 118 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 118 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 119 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 119 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gatgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 120 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 120 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Asp Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 121 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 121 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 caagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 122 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 122 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Gln Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 123 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 123 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 124 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 124 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 125 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 125 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 126 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 126 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 127 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 127 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 128 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 128 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 129 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 129 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 130 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 130 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 131 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 131 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gatgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagcat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 132 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 132 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Asp Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 133 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 133 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 134 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 134 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 135 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 135 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 136 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 136 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 137 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 137 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 138 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 138 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 139 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 139 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 140 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 140 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 141 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 141 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 142 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 142 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 143 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 143 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 144 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 144 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 145 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 145 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagcat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 146 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 146 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 147 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 147 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 148 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 148 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 149 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 149 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 150 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 150 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 151 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 151 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacaggatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 152 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 152 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Arg Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 153 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 153 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 154 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 154 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 155 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 155 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 156 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 156 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 157 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 157 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 158 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 158 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 159 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 159 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacaggatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 160 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 160 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Arg Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 161 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 161 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gatgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 162 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 162 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Asp Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 163 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 163 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 164 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 164 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 165 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 165 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gatgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggatatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacaggatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 166 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 166 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Asp Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Arg Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 167 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 167 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 caagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 168 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 168 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Gln Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 169 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 169 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 170 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 170 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 171 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 171 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 172 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 172 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 173 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 173 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 174 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 174 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 175 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 175 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat gatgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 176 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 176 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Asp Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 177 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 177 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 178 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 178 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 179 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 179 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacaggatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 180 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 180 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Arg Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 181 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 181 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 182 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 182 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 183 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 183 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 184 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 184 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 185 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 185 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 186 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 186 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 187 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 187 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagcat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 188 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 188 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 189 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 189 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacaggatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 190 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 190 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Arg Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 191 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 191 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat gatgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 192 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 192 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Asp Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 193 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 193 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacaggatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 194 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 194 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Arg Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 195 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 195 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 196 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 196 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 197 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 197 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat gatgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacaggatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 198 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 198 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Asp Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Arg Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 199 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 199 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagcat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 200 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 200 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 201 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 201 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacaggatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 202 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 202 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Arg Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 203 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 203 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 204 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 204 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 205 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 205 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacaggatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 206 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 206 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Arg Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 207 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 207 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gatgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggc 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 208 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 208 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Asp Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 209 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 209 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 210 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 210 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 211 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 211 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 212 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 212 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 213 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 213 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 214 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 214 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 215 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 215 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgatgctc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 216 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 216 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Ala Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 217 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 217 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcacagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 218 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 218 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 219 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 219 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 220 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 220 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 221 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 221 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctgc aagacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagacat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 222 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 222 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Gln 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 223 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 223 agcaatatgt gggtgattgg caaaagcaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gctgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggcaaacg atccgcgccg ccaactggtg 960 gataaactga ccagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tgggaaacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagcat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 224 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 224 Ser Asn Met Trp Val Ile Gly Lys Ser Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Ala Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Thr Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Glu 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 225 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 225 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 226 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 226 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 227 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 227 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccag taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 228 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 228 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Ser Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 229 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 229 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagcta ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 230 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 230 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Ala Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 231 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 231 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctct ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 232 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 232 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 233 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 233 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 234 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 234 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 235 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 235 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 236 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 236 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 237 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 237 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 238 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 238 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 239 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 239 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcccttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 240 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 240 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 241 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 241 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 242 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 242 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 243 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 243 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 244 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 244 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 245 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 245 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 246 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 246 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 247 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 247 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 248 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 248 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 249 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 249 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctct ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 250 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 250 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 251 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 251 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 252 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 252 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 253 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 253 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 254 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 254 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 255 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 255 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagtacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 256 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 256 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu Tyr Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 257 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 257 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaacaga ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 258 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 258 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Gln Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 259 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 259 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 260 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 260 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 261 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 261 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 262 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 262 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 263 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 263 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 264 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 264 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 265 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 265 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 266 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 266 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 267 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 267 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 268 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 268 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 269 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 269 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 270 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 270 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 271 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 271 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 272 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 272 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 273 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 273 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 274 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 274 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 275 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 275 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 276 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 276 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 277 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 277 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 278 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 278 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 279 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 279 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcttgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagcta ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 280 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 280 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Ala Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 281 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 281 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 282 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 282 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 283 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 283 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 284 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 284 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 285 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 285 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaaaaca ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 286 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 286 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asn Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 287 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 287 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 288 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 288 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 289 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 289 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 290 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 290 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 291 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 291 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 292 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 292 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 293 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 293 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 294 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 294 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 295 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 295 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 296 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 296 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 297 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 297 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 298 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 298 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 299 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 299 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 300 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 300 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 301 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 301 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 302 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 302 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 303 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 303 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 304 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 304 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 305 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 305 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 306 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 306 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 307 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 307 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 308 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 308 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 309 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 309 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 310 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 310 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 311 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 311 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaacaga ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 312 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 312 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Gln Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 313 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 313 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaggtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 314 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 314 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 315 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 315 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 316 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 316 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 317 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 317 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgagtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 318 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 318 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Glu Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 319 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 319 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 320 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 320 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 321 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 321 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctct ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 322 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 322 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 323 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 323 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 324 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 324 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 325 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 325 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 326 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 326 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 327 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 327 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 328 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 328 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 329 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 329 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcccttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 330 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 330 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 331 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 331 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca acgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 332 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 332 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 333 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 333 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 334 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 334 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 335 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 335 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaaaaca ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 336 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 336 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asn Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 337 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 337 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 338 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 338 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 339 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 339 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 ccaggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 340 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 340 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 341 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 341 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 342 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 342 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 343 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 343 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 344 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 344 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 345 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 345 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 346 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 346 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 347 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 347 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctct ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 348 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 348 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 349 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 349 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcccttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 350 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 350 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 351 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 351 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctct ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 352 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 352 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 353 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 353 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 354 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 354 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 355 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 355 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 356 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 356 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 357 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 357 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 358 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 358 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 359 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 359 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctct ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 360 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 360 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 361 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 361 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 362 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 362 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 363 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 363 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 364 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 364 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 365 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 365 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 366 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 366 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 367 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 367 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggatatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 368 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 368 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 369 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 369 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac ttcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 370 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 370 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ser Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 371 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 371 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 372 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 372 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 373 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 373 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctct ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 374 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 374 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 375 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 375 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctct ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 376 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 376 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 377 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 377 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcccttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 378 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 378 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 379 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 379 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 380 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 380 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 381 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 381 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcccttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 382 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 382 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 383 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 383 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 384 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 384 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 385 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 385 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctct ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 386 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 386 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 387 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 387 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 388 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 388 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 389 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 389 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaaca ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 390 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 390 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asn Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 391 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 391 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaacaga ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 392 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 392 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Gln Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 393 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 393 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 394 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 394 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 395 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 395 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 396 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 396 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 397 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 397 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaaca ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 398 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 398 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asn Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 399 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 399 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctca acgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 400 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 400 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 401 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 401 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 402 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 402 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 403 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 403 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta gcggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 404 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 404 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 405 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 405 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 406 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 406 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 407 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 407 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gcggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcccttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 408 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 408 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 409 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 409 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaacaga ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 410 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 410 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Gln Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 411 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 411 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcact ttgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca ccggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcctt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 412 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 412 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp His Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Thr Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 413 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 413 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgacaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccctg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaacaga ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 414 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 414 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Leu Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Gln Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 415 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 415 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcatgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 416 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 416 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 417 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 417 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 418 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 418 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 419 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 419 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggatggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 420 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 420 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 421 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 421 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 422 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 422 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 423 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 423 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 424 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 424 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 425 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 425 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 426 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 426 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 427 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 427 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgggttgta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 428 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 428 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Gly Cys Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 429 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 429 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagtgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 430 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 430 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 431 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 431 agcaatatgt gggtgattgg caaagataaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 432 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 432 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 433 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 433 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggt 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 434 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 434 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 435 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 435 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 436 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 436 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 437 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 437 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc aatggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 438 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 438 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 439 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 439 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc gttggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 440 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 440 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Arg Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 441 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 441 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 442 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 442 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 443 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 443 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa agataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 444 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 444 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 445 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 445 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 446 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 446 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 447 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 447 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 448 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 448 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 449 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 449 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc taaagaggca cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca acaccagcga acttgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 450 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 450 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Asn 690 695 700 Thr Ser Glu Leu Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 451 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 451 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaacagat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccca gaacttcttc 1380 ggcgtgccgc aggcggcagc taaagagtgc cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 452 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 452 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Cys Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 453 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 453 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccca gaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 454 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 454 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 455 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 455 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctga aagcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccca gaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggca cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca acaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 456 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 456 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Lys Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Asn 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 457 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 457 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaacagat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tactcggcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccca gaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca acaccagcga acttgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 458 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 458 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Asn 690 695 700 Thr Ser Glu Leu Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 459 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 459 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccaccatg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc taaagaggaa cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 460 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 460 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Met Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Glu Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 461 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 461 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc taaagaggca cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 462 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 462 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 463 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 463 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc taaagaggaa cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 464 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 464 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Glu Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 465 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 465 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc taaagaggaa cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaaaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca acaccaccga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 466 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 466 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Glu Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Lys Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Asn 690 695 700 Thr Thr Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 467 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 467 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaacagat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacttggcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc taaagaggaa cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaaaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga acttgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 468 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 468 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Leu Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Glu Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Lys Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Leu Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 469 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 469 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccca gaacttcttc 1380 ggcgtgccgc aggcggcagc taaagagtgc cgtcatcagt gcgagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 470 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 470 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Cys Arg His Gln Cys Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 471 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 471 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc taaagaggaa cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaaaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca acaccaccga acttgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 472 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 472 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Glu Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Lys Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Asn 690 695 700 Thr Thr Glu Leu Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 473 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 473 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tactcggcca gtggctatga aaccaccatg 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc taaagaggca cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaaaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 474 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 474 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Met Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Lys Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 475 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 475 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggcagtct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcatg gaaagagtgc cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca acaccagcga acttgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 476 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 476 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Trp Lys Glu Cys Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Asn 690 695 700 Thr Ser Glu Leu Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 477 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 477 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaacagat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacttggcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccca gaacttcttc 1380 ggcgtgccgc aggcggcagc taaagaggaa cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaaaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcact aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 478 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 478 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Leu Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Glu Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Lys Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 479 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 479 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaacagat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggca cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaaaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 480 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 480 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Lys Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 481 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 481 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaacagat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tactcggcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc taaagagtgc cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga acttgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 482 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 482 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Cys Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Leu Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 483 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 483 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggca cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaaaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 484 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 484 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Lys Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 485 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 485 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 486 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 486 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 487 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 487 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 488 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 488 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 489 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 489 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 490 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 490 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 491 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 491 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 492 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 492 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 493 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 493 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 494 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 494 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 495 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 495 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 496 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 496 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 497 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 497 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 498 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 498 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 499 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 499 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 500 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 500 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 501 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 501 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 502 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 502 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 503 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 503 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 504 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 504 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 505 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 505 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactatttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 506 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 506 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 507 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 507 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 508 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 508 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 509 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 509 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 510 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 510 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 511 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 511 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 gacaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 512 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 512 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Asp Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 513 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 513 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 514 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 514 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 515 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 515 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 516 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 516 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 517 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 517 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 518 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 518 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 519 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 519 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 520 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 520 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 521 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 521 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 522 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 522 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 523 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 523 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagacccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 524 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 524 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Arg Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 525 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 525 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 526 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 526 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 527 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 527 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 528 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 528 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 529 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 529 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 530 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 530 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 531 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 531 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 532 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 532 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 533 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 533 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaatac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 534 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 534 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Asn Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 535 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 535 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 536 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 536 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 537 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 537 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 538 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 538 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 539 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 539 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 540 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 540 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 541 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 541 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcagtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 542 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 542 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 543 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 543 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 544 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 544 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 545 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 545 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 546 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 546 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 547 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 547 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 548 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 548 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 549 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 549 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 550 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 550 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 551 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 551 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 552 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 552 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 553 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 553 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 554 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 554 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 555 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 555 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 556 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 556 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 557 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 557 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 558 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 558 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 559 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 559 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 560 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 560 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 561 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 561 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcagtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 562 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 562 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 563 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 563 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 564 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 564 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 565 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 565 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 566 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 566 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 567 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 567 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 568 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 568 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 569 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 569 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 570 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 570 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 571 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 571 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 572 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 572 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 573 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 573 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 574 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 574 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 575 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 575 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 576 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 576 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 577 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 577 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 578 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 578 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 579 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 579 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 580 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 580 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 581 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 581 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 582 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 582 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 583 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 583 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 584 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 584 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 585 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 585 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 586 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 586 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 587 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 587 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 588 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 588 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 589 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 589 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 590 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 590 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 591 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 591 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 592 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 592 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 593 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 593 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 594 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 594 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 595 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 595 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 596 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 596 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 597 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 597 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc agtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 598 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 598 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 599 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 599 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcagtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 600 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 600 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 601 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 601 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcagtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acacaaacgg cgtaa 2295 <210> 602 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 602 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 603 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 603 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 604 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 604 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 605 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 605 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 606 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 606 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 607 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 607 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca attggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 608 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 608 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 609 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 609 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 610 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 610 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 611 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 611 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 612 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 612 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 613 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 613 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 614 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 614 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 615 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 615 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 616 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 616 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 617 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 617 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 618 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 618 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 619 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 619 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata aatggaaaac ccccgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 620 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 620 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 621 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 621 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcagtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 622 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 622 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 623 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 623 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 624 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 624 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 625 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 625 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 626 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 626 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 627 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 627 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 628 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 628 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 629 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 629 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 630 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 630 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 631 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 631 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 632 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 632 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 633 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 633 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 634 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 634 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 635 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 635 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 636 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 636 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 637 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 637 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 638 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 638 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 639 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 639 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 640 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 640 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 641 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 641 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 642 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 642 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 643 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 643 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 644 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 644 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 645 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 645 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 646 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 646 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 647 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 647 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 648 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 648 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 649 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 649 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 650 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 650 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 651 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 651 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 652 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 652 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 653 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 653 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcagtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 654 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 654 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 655 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 655 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 656 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 656 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 657 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 657 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgatggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 658 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 658 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Met Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 659 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 659 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 660 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 660 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 661 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 661 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 662 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 662 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 663 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 663 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 664 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 664 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 665 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 665 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 666 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 666 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 667 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 667 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcagtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 668 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 668 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 669 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 669 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 670 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 670 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 671 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 671 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 672 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 672 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 673 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 673 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 674 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 674 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 675 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 675 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 676 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 676 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Glu Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 677 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 677 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 678 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 678 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 679 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 679 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 680 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 680 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 681 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 681 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 682 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 682 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 683 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 683 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 684 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 684 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 685 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 685 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcagtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 686 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 686 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 687 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 687 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 688 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 688 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 689 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 689 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 690 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 690 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 691 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 691 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 692 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 692 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 693 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 693 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 694 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 694 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 695 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 695 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 696 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 696 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 697 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 697 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 698 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 698 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 699 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 699 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcca 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 700 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 700 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 701 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 701 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 702 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 702 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 703 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 703 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 704 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 704 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 705 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 705 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 706 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 706 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 707 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 707 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 708 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 708 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 709 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 709 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 710 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 710 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 711 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 711 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 712 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 712 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 713 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 713 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 714 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 714 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 715 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 715 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 716 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 716 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 717 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 717 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 718 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 718 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 719 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 719 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 720 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 720 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 721 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 721 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 722 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 722 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 723 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 723 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 724 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 724 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 725 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 725 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 726 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 726 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 727 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 727 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaata aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 728 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 728 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asn Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 729 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 729 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 730 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 730 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 731 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 731 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 732 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 732 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 733 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 733 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 734 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 734 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 735 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 735 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 736 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 736 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 737 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 737 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 738 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 738 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 739 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 739 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 740 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 740 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 741 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 741 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 742 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 742 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 743 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 743 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 744 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 744 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 745 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 745 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 746 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 746 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 747 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 747 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 748 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 748 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 749 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 749 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 750 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 750 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 751 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 751 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 752 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 752 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 753 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 753 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 754 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 754 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 755 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 755 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 756 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 756 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 757 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 757 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 758 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 758 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 759 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 759 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 760 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 760 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 761 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 761 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 762 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 762 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 763 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 763 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 764 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 764 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 765 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 765 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 766 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 766 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 767 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 767 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 768 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 768 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 769 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 769 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 770 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 770 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 771 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 771 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 772 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 772 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 773 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 773 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgata aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 774 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 774 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 775 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 775 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 776 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 776 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 777 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 777 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 778 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 778 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 779 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 779 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 780 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 780 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 781 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 781 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 782 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 782 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 783 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 783 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 784 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 784 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 785 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 785 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 786 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 786 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 787 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 787 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 788 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 788 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 789 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 789 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 790 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 790 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 791 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 791 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 792 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 792 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 793 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 793 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 794 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 794 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 795 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 795 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 796 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 796 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 797 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 797 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 798 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 798 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 799 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 799 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 800 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 800 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 801 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 801 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 802 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 802 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 803 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 803 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 804 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 804 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 805 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 805 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 806 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 806 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 807 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 807 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc agtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 808 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 808 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gln Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 809 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 809 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 810 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 810 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 811 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 811 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 812 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 812 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 813 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 813 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggatgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 814 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 814 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Asp Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 815 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 815 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 816 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 816 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 817 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 817 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggca atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 818 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 818 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Ser Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 819 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 819 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 820 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 820 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 821 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 821 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 822 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 822 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 823 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 823 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 824 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 824 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 825 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 825 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcggcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 826 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 826 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Arg Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 827 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 827 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcgatc gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 828 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 828 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Asp Arg Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 829 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 829 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 830 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 830 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 831 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 831 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 832 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 832 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 833 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 833 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca aatggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gagcgaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 834 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 834 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Lys Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Ser Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 835 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 835 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggagggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gttggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc tgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagact 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 836 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 836 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 837 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 837 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tggacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca catggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaaaa ggataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 838 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 838 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asp Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Thr Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Lys Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 839 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 839 agcaatatgt gggtgattgg caaagacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttcacgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccc tgtggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagggc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcgatc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc ggaagaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 840 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 840 Ser Asn Met Trp Val Ile Gly Lys Asp Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe His Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Leu Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Gly 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Asp Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Glu Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 841 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 841 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 842 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 842 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 843 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 843 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 844 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 844 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 845 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 845 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 846 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 846 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 847 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 847 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 848 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 848 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 849 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 849 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 850 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 850 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 851 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 851 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 852 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 852 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 853 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 853 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 854 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 854 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 855 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 855 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccca aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcaaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 856 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 856 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 857 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 857 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 858 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 858 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 859 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 859 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 860 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 860 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 861 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 861 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 862 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 862 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 863 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 863 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 864 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 864 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 865 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 865 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 866 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 866 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 867 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 867 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaatggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 868 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 868 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 869 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 869 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 870 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 870 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 871 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 871 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccca aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 872 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 872 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 873 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 873 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccca aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 874 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 874 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 875 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 875 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 876 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 876 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 877 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 877 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccca aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 878 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 878 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 879 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 879 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 880 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 880 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 881 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 881 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 882 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 882 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 883 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 883 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 884 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 884 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 885 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 885 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 886 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 886 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 887 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 887 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 888 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 888 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 889 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 889 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 890 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 890 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 891 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 891 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 892 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 892 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 893 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 893 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 894 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 894 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 895 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 895 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccca aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 896 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 896 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 897 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 897 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 898 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 898 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 899 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 899 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 900 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 900 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 901 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 901 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 902 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 902 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 903 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 903 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccca aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 904 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 904 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 905 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 905 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 906 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 906 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 907 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 907 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 908 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 908 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 909 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 909 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 910 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 910 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 911 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 911 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 912 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 912 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 913 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 913 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 914 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 914 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 915 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 915 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 916 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 916 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 917 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 917 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 918 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 918 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 919 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 919 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 920 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 920 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 921 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 921 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 922 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 922 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 923 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 923 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 924 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 924 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 925 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 925 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 926 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 926 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 927 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 927 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 928 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 928 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 929 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 929 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 930 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 930 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 931 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 931 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 932 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 932 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 933 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 933 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 934 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 934 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 935 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 935 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccca aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 936 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 936 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 937 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 937 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 938 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 938 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 939 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 939 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccca aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 940 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 940 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 941 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 941 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 942 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 942 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 943 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 943 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccca aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 944 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 944 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 945 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 945 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaatgggat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattaca atgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccca aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 946 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 946 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Trp Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Asn Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Gln Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 947 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 947 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 948 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 948 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 949 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 949 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgtttct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg aaacttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 950 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 950 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu His Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 951 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 951 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt tcggttggac tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cacggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaactgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgattact ttgcgcgtct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaaaagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagccgta ccggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcaaa gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg aaacttacct tccgggccaa caacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaatactt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaaagtat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 952 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 952 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Tyr Phe Ala Arg 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Lys 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Arg Thr Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Lys Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Lys Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Tyr Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 953 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 953 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct cgccagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 954 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 954 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Arg Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 955 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 955 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ctgtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 956 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 956 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Leu Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 957 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 957 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 gcgtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 958 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 958 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Ala Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 959 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 959 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 960 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 960 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 961 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 961 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gtgggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 962 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 962 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Val Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 963 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 963 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt aaaggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 964 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 964 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Lys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 965 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 965 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gaaggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 966 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 966 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Glu Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 967 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 967 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 968 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 968 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 969 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 969 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccct ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 970 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 970 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Leu Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 971 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 971 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccac taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 972 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 972 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Thr Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 973 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 973 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctactggcgt ttacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc cgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 974 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 974 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Phe Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Pro Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 975 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 975 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 976 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 976 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 977 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 977 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 gtttctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 978 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 978 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Val Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 979 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 979 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 cattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 980 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 980 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg His Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 981 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 981 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tgttctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 982 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 982 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Cys Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 983 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 983 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 atttctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 984 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 984 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Ile Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 985 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 985 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 atgtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 986 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 986 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Met Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 987 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 987 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcacctt tgcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 988 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 988 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Phe Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 989 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 989 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccgg ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 990 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 990 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Gly Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 991 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 991 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgcgccggcg tttacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 992 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 992 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Ala Pro Ala Phe Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 993 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 993 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgcgccggcg ttgacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 994 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 994 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Ala Pro Ala Leu Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 995 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 995 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgcgccggcg gtgacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 996 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 996 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Ala Pro Ala Val Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 997 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 997 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgcgccggcg actacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 998 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 998 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Ala Pro Ala Thr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 999 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 999 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgcgccggcg tgtacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1000 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1000 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Ala Pro Ala Cys Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1001 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1001 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgcgccggcg aatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1002 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1002 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Ala Pro Ala Asn Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1003 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1003 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgcgccggcg atgacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1004 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1004 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Ala Pro Ala Met Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1005 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1005 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgcgccggcg aagacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1006 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1006 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Ala Pro Ala Lys Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1007 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1007 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tggacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cataa 2295 <210> 1008 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1008 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Trp Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1009 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1009 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1010 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1010 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1011 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1011 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tgtacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggcccgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1012 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1012 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Cys Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Pro Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Tyr Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1013 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1013 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1014 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1014 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1015 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1015 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atgtgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1016 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1016 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Val Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Tyr Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1017 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1017 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tggacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcacatt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgtg ctactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaggc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1018 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1018 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Trp Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Thr Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Ala Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Gly Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1019 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1019 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tggacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1020 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1020 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Trp Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1021 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1021 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctacaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1022 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1022 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1023 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1023 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcacatt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1024 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1024 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Thr Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1025 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1025 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tggacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1026 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1026 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Trp Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1027 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1027 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt tcacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacggtc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcacctt agcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1028 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1028 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Phe Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Val Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Leu Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1029 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1029 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg ttaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcagaaaacg atccgcgccg ccaactggtg 960 gataaactgg ctagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcacctt tgcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1030 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1030 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Phe Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1031 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1031 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tggacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1032 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1032 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Trp Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Tyr Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1033 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1033 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tggacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcacctt agcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1034 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1034 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Trp Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Leu Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1035 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1035 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1036 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1036 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1037 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1037 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cacgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccgcccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1038 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1038 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Thr Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Ala Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1039 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1039 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 aataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 cattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1040 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1040 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asn Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg His Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1041 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1041 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccta taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1042 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1042 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Tyr Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1043 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1043 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcacaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1044 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1044 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1045 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1045 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggtg tacgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcgttcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1046 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1046 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Cys Thr Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Phe Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1047 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1047 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcacatt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1048 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1048 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Thr Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1049 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1049 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctatg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac tgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcacctt agcgaaccgt 2100 tattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1050 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1050 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Leu Ala Asn Arg Tyr Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1051 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1051 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcacatt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gcggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1052 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1052 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Thr Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Arg Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1053 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1053 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcacatt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 atctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1054 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1054 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Thr Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Ile Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1055 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1055 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcagccg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattccattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcacctt tgcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1056 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1056 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Pro Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Phe Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1057 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1057 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcctctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gatagactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcacctt agcgaaccgt 2100 gtctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1058 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1058 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Leu Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Arg Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Leu Ala Asn Arg Val Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1059 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1059 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcctctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttactt tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 cattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1060 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1060 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Leu Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg His Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1061 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1061 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tgtacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcctctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 cattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1062 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1062 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Cys Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Leu Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg His Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1063 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1063 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1064 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1064 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1065 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1065 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaagccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt ttcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1066 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1066 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Ala Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Phe Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Tyr Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1067 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1067 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcacatt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac caggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1068 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1068 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Thr Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Gln Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1069 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1069 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaacac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1070 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1070 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Thr Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1071 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1071 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt tcacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1072 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1072 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Phe Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1073 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1073 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcctctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgttaggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcgaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1074 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1074 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Leu Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Arg Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Glu Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Tyr Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1075 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1075 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcacatt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaagat gtacgagagc tttggccgta aatcgctgtg gttaacgccc 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcacctt agcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1076 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1076 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Thr Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Leu Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1077 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1077 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tatggtgata aggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1078 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1078 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Tyr Gly Asp Lys Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1079 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1079 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg gcaacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1080 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1080 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Ala Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1081 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1081 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt aagggtgatc ttgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1082 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1082 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Lys Gly Asp Leu Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1083 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1083 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt aagggtgata atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaccagat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1084 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1084 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Lys Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Pro Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1085 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1085 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg taccgttacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1086 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1086 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Arg 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1087 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1087 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccttgggt ggtggtgata cagtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1088 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1088 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Leu Gly Gly Gly Asp Thr Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1089 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1089 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atcatgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1090 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1090 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp His Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1091 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1091 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 gcagcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1092 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1092 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Ala Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1093 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1093 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggtgtcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1094 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1094 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Cys Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1095 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1095 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttg gggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1096 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1096 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Trp Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1097 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1097 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gaaggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1098 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1098 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Glu Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1099 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1099 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gagggtgata cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1100 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1100 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Glu Gly Asp Thr Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1101 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1101 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tggggtgata ctgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1102 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1102 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Trp Gly Asp Thr Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1103 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1103 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tggggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1104 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1104 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Trp Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1105 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1105 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tggggtgatt cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1106 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1106 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Trp Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1107 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1107 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gatggtgatc atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1108 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1108 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Asp Gly Asp His Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1109 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1109 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gatggtgata tggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1110 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1110 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Asp Gly Asp Met Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1111 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1111 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gatggtgatc ttgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1112 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1112 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Asp Gly Asp Leu Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1113 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1113 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gagggtgata ctgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggtg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1114 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1114 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Glu Gly Asp Thr Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Val Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1115 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1115 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1116 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1116 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Trp Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1117 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1117 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gatggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1118 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1118 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Asp Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1119 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1119 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcgt atatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1120 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1120 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Tyr Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1121 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1121 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt catggtgata tggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1122 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1122 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Met Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1123 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1123 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt attggtgatc aggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atagcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1124 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1124 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Ile Gly Asp Gln Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Ser Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1125 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1125 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt catggtgatc atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1126 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1126 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp His Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1127 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1127 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgtgg gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1128 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1128 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Trp Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1129 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1129 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt attggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggtgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1130 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1130 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Ile Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Val Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1131 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1131 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt aatggtgatc aggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1132 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1132 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Asn Gly Asp Gln Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1133 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1133 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt aatggtgatg cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1134 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1134 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Asn Gly Asp Ala Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1135 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1135 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt catggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1136 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1136 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1137 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1137 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg ttgacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat caactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag cgactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac caccaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccgcccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac caggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaggc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1138 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1138 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Leu Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Ala Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Thr Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Ala Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Gln Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Gly Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1139 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1139 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt aatggtgatg ttgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctgcccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1140 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1140 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Asn Gly Asp Val Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Cys Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1141 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1141 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt catggtgatg gggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1142 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1142 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1143 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1143 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt catggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacacacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1144 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1144 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1145 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1145 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt aatggtgatt ctgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1146 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1146 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Asn Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1147 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1147 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg aggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1148 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1148 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Glu Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1149 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1149 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tatggtgatt tggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1150 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1150 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Tyr Gly Asp Leu Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1151 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1151 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt catggtgatt ctgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1152 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1152 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1153 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1153 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt catggtgatg ctgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1154 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1154 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly His Gly Asp Ala Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1155 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1155 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cttggtgata tggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1156 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1156 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Leu Gly Asp Met Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1157 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1157 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt actggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1158 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1158 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Thr Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1159 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1159 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa aaggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1160 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1160 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Lys 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1161 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1161 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgataatt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1162 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1162 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Asn Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1163 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1163 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcagtttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1164 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1164 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ser Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1165 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1165 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagaaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1166 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1166 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Glu Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1167 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1167 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tccatttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1168 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1168 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe His Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1169 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1169 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatatgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1170 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1170 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Met Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1171 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1171 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccat atggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1172 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1172 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Tyr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1173 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1173 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcaat tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1174 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1174 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Gln Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1175 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1175 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact taaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1176 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1176 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1177 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1177 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tctatttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1178 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1178 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Tyr Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1179 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1179 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttctat 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1180 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1180 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Tyr Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1181 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1181 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1182 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1182 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1183 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1183 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgtgtat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1184 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1184 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Cys 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1185 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1185 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacatg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1186 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1186 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1187 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1187 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggccg tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1188 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1188 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Arg Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1189 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1189 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgaaatg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1190 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1190 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Lys Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1191 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1191 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgaggtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1192 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1192 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Arg Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1193 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1193 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1194 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1194 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1195 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1195 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat caactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag cgactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgccaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa aaggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacctg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1196 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1196 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Ala Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Lys 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1197 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1197 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat caactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag cgactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgccaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacctg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1198 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1198 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Ala Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1199 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1199 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat caactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgccaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa aaggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacctg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1200 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1200 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Lys 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1201 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1201 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat caactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag cgactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgccaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacctg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1202 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1202 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Ala Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1203 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1203 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag cgactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgccaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacctg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1204 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1204 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Ala Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1205 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1205 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat caactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag cgactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgccaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacctg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1206 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1206 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Ala Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1207 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1207 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat caactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgccaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacctg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1208 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1208 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1209 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1209 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat caactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag cgactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgccaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacctg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1210 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1210 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Ala Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1211 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1211 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tacgccggcg tatacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat caactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag cgactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgccaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaaca acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacctg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1212 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1212 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Thr Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Ala Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asn Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1213 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1213 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1214 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1214 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1215 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1215 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat gcggcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1216 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1216 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1217 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1217 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gcggcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1218 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1218 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1219 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1219 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat gcggcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1220 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1220 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1221 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1221 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1222 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1222 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1223 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1223 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1224 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1224 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1225 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1225 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1226 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1226 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1227 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1227 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gtgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1228 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1228 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1229 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1229 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1230 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1230 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1231 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1231 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gcggcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa aaggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1232 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1232 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Lys 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1233 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1233 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1234 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1234 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1235 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1235 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gcggcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1236 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1236 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1237 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1237 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gcggcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa aaggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1238 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1238 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Lys 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1239 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1239 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gcggcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1240 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1240 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1241 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1241 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gcggcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1242 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1242 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1243 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1243 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1244 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1244 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1245 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1245 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcact gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1246 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1246 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1247 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1247 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacctg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1248 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1248 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1249 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1249 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcgaag agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat gcggcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggtgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1250 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1250 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Glu Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Ala Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1251 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1251 agcttgatgt gggtgattgg caaaaacaaa gccgcggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttatcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgaaat cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcacca actggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggtg atcgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaagatat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1252 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1252 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Ala Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Tyr Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Lys Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Asn Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asp Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Asp Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1253 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1253 agcttgatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga tgagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1254 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1254 Ser Leu Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Glu Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1255 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1255 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa aaggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1256 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1256 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Lys 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1257 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1257 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcatt gaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1258 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1258 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Leu Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1259 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1259 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 ggaccgcagt atggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgacatg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1260 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1260 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Tyr Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Thr Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1261 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1261 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1262 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1262 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1263 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1263 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgcgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggcaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtgatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacaa agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagataaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1264 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1264 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Ala Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Lys Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1265 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1265 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1266 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1266 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Tyr Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1267 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1267 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ctgggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1268 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1268 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Leu Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1269 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1269 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgtggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1270 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1270 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1271 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1271 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cgtggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1272 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1272 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Arg Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1273 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1273 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcacttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1274 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1274 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Thr Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1275 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1275 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1276 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1276 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1277 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1277 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcctgtt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1278 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1278 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Leu Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1279 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1279 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctactggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1280 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1280 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1281 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1281 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctactggcgt ttacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1282 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1282 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Phe Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1283 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1283 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt ggtggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1284 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1284 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1285 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1285 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cagggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggt cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1286 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1286 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gln Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Ser Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1287 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1287 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1288 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1288 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1289 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1289 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1290 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1290 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1291 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1291 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 gtttctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1292 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1292 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Val Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1293 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1293 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgtcggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcctctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgtccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaagcg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1294 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1294 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Ser Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Leu Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Ser 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Ser Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1295 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1295 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcgg 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gggggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1296 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1296 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Arg Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Gly Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1297 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1297 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg cacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcatctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttctctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1298 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1298 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala His Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Ile Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1299 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1299 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcctctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaaccgt 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggtaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1300 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1300 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Leu Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Arg 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1301 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1301 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcacatt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tgcggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgtgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctattcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1302 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1302 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Thr Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Cys Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1303 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1303 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagg ctggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt cttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1304 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1304 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Ala Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Leu Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1305 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1305 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcctctt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatac ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1306 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1306 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Leu Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Thr Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1307 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1307 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttgtta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1308 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1308 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Cys Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1309 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1309 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 aatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1310 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1310 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asn Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1311 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1311 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcacatt tggtcacaac 180 ggcaccattt catggggatc caccgccggt tttggtgatg gtgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 tggtctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1312 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1312 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Thr Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Phe Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Trp Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1313 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1313 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1314 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1314 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1315 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1315 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1316 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1316 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1317 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1317 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1318 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1318 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1319 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1319 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tgggggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1320 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1320 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1321 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1321 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1322 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1322 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1323 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1323 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1324 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1324 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1325 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1325 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1326 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1326 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1327 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1327 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1328 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1328 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1329 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1329 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1330 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1330 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1331 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1331 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1332 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1332 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1333 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1333 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1334 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1334 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1335 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1335 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1336 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1336 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1337 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1337 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1338 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1338 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1339 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1339 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1340 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1340 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1341 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1341 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1342 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1342 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1343 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1343 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1344 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1344 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1345 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1345 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1346 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1346 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1347 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1347 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1348 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1348 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1349 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1349 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1350 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1350 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1351 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1351 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1352 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1352 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1353 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1353 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1354 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1354 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1355 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1355 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1356 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1356 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1357 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1357 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1358 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1358 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1359 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1359 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1360 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1360 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1361 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1361 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1362 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1362 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1363 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1363 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tgggggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1364 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1364 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1365 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1365 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1366 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1366 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1367 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1367 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacggaccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1368 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1368 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1369 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1369 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1370 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1370 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1371 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1371 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1372 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1372 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1373 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1373 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1374 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1374 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1375 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1375 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1376 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1376 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1377 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1377 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1378 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1378 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1379 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1379 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1380 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1380 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1381 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1381 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1382 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1382 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1383 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1383 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1384 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1384 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1385 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1385 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1386 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1386 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1387 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1387 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 tgggggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1388 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1388 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1389 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1389 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1390 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1390 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1391 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1391 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 tgggggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1392 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1392 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1393 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1393 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1394 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1394 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1395 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1395 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1396 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1396 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1397 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1397 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1398 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1398 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1399 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1399 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1400 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1400 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1401 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1401 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1402 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1402 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1403 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1403 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1404 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1404 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1405 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1405 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1406 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1406 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1407 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1407 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1408 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1408 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1409 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1409 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1410 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1410 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1411 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1411 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1412 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1412 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1413 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1413 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1414 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1414 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1415 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1415 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1416 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1416 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1417 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1417 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1418 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1418 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1419 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1419 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1420 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1420 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1421 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1421 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1422 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1422 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1423 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1423 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1424 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1424 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1425 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1425 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1426 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1426 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1427 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1427 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1428 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1428 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1429 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1429 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1430 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1430 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1431 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1431 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1432 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1432 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1433 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1433 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgtcatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1434 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1434 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Val Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1435 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1435 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1436 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1436 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1437 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1437 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tgggggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1438 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1438 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1439 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1439 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1440 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1440 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1441 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1441 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1442 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1442 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1443 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1443 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1444 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1444 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1445 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1445 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1446 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1446 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1447 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1447 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1448 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1448 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1449 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1449 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1450 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1450 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1451 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1451 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1452 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1452 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1453 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1453 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1454 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1454 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1455 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1455 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1456 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1456 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1457 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1457 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1458 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1458 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1459 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1459 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tgggttgatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1460 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1460 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Leu Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1461 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1461 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1462 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1462 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1463 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1463 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1464 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1464 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1465 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1465 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1466 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1466 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1467 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1467 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1468 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1468 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1469 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1469 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1470 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1470 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1471 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1471 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1472 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1472 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1473 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1473 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1474 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1474 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1475 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1475 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1476 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1476 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1477 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1477 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1478 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1478 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1479 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1479 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tgggggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1480 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1480 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1481 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1481 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1482 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1482 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1483 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1483 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1484 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1484 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1485 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1485 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1486 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1486 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1487 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1487 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1488 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1488 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1489 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1489 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1490 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1490 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1491 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1491 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1492 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1492 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1493 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1493 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1494 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1494 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1495 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1495 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1496 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1496 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1497 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1497 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1498 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1498 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1499 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1499 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1500 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1500 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1501 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1501 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1502 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1502 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1503 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1503 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1504 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1504 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1505 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1505 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1506 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1506 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1507 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1507 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1508 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1508 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1509 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1509 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgt ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1510 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1510 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Val Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1511 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1511 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1512 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1512 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1513 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1513 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1514 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1514 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1515 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1515 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1516 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1516 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1517 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1517 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1518 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1518 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1519 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1519 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1520 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1520 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1521 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1521 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1522 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1522 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1523 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1523 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1524 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1524 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1525 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1525 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1526 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1526 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1527 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1527 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1528 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1528 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1529 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1529 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1530 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1530 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1531 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1531 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1532 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1532 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1533 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1533 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1534 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1534 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1535 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1535 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1536 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1536 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1537 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1537 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1538 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1538 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1539 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1539 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1540 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1540 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1541 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1541 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1542 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1542 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1543 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1543 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1544 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1544 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1545 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1545 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1546 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1546 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1547 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1547 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1548 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1548 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1549 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1549 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1550 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1550 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1551 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1551 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 aaagggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1552 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1552 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1553 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1553 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1554 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1554 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1555 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1555 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1556 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1556 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1557 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1557 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1558 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1558 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1559 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1559 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1560 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1560 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1561 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1561 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1562 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1562 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1563 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1563 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1564 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1564 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1565 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1565 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1566 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1566 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1567 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1567 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1568 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1568 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1569 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1569 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1570 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1570 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1571 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1571 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1572 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1572 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1573 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1573 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tgggggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1574 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1574 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1575 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1575 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1576 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1576 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1577 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1577 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggctgt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1578 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1578 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Cys Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1579 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1579 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1580 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1580 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1581 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1581 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1582 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1582 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1583 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1583 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1584 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1584 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1585 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1585 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1586 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1586 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1587 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1587 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1588 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1588 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1589 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1589 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1590 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1590 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1591 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1591 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1592 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1592 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1593 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1593 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1594 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1594 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1595 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1595 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1596 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1596 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1597 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1597 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 aaagggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1598 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1598 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1599 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1599 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 tgggggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1600 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1600 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1601 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1601 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1602 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1602 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1603 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1603 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1604 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1604 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1605 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1605 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 aaagggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1606 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1606 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1607 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1607 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 tgggggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1608 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1608 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1609 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1609 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1610 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1610 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1611 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1611 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1612 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1612 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1613 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1613 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tgggggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1614 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1614 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1615 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1615 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 aaagggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1616 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1616 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1617 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1617 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1618 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1618 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1619 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1619 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1620 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1620 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1621 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1621 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1622 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1622 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1623 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1623 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1624 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1624 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1625 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1625 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1626 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1626 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1627 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1627 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1628 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1628 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1629 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1629 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1630 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1630 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1631 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1631 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1632 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1632 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1633 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1633 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1634 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1634 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1635 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1635 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1636 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1636 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1637 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1637 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1638 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1638 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1639 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1639 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1640 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1640 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1641 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1641 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 aatgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1642 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1642 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asn Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1643 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1643 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1644 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1644 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1645 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1645 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1646 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1646 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1647 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1647 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tgggggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1648 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1648 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1649 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1649 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1650 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1650 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1651 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1651 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tgggggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1652 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1652 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1653 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1653 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1654 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1654 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1655 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1655 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1656 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1656 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1657 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1657 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1658 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1658 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1659 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1659 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgcatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1660 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1660 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1661 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1661 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1662 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1662 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1663 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1663 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1664 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1664 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1665 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1665 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1666 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1666 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1667 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1667 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1668 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1668 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1669 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1669 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1670 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1670 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1671 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1671 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1672 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1672 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1673 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1673 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1674 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1674 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1675 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1675 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1676 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1676 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1677 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1677 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggtg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1678 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1678 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Val Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1679 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1679 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1680 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1680 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1681 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1681 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 aaagggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1682 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1682 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1683 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1683 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1684 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1684 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1685 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1685 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1686 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1686 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1687 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1687 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1688 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1688 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1689 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1689 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1690 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1690 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1691 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1691 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1692 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1692 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1693 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1693 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tgggggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1694 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1694 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Trp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1695 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1695 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1696 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1696 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1697 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1697 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatc cggtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1698 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1698 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Pro Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1699 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1699 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1700 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1700 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Gly Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1701 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1701 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt ggggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1702 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1702 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Trp Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1703 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1703 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata acgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt gggcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggttgctgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1704 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1704 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asn Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Trp Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Cys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1705 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1705 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgtgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 tttgggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1706 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1706 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1707 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1707 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct tcgtgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 aaagggccac cgggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1708 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1708 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Arg Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Pro 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1709 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1709 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggac cgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgata gcgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcggcttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagatt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1710 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1710 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Thr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Ser Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Gly Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Ile Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1711 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1711 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1712 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1712 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1713 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1713 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actagtacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1714 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1714 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ser Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1715 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1715 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacga tgaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1716 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1716 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Met Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1717 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1717 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaatgga ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1718 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1718 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Trp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1719 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1719 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatcgtt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1720 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1720 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Arg Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1721 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1721 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagat gtactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1722 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1722 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Met Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1723 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1723 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagcg ttactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1724 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1724 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Arg Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1725 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1725 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1726 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1726 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asn Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1727 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1727 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat cttgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1728 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1728 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Leu Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1729 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1729 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcgttgcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1730 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1730 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Val Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1731 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1731 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 ttggggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1732 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1732 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Leu Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1733 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1733 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 cgtgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1734 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1734 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Arg Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1735 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1735 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actggtacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1736 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1736 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Gly Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1737 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1737 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcaag taaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1738 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1738 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ser Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1739 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1739 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc tgatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1740 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1740 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1741 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1741 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgtatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1742 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1742 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Tyr 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1743 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1743 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagtta ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1744 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1744 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Val Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1745 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1745 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gccatgcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1746 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1746 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly His Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1747 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1747 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagcaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1748 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1748 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Ala Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1749 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1749 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 caagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1750 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1750 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Gln Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1751 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1751 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaaggtt tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1752 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1752 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Val Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1753 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1753 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gctgtgcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1754 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1754 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Cys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1755 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1755 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1756 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1756 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1757 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1757 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1758 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1758 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asn Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1759 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1759 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatatgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1760 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1760 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Met Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1761 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1761 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgcatct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1762 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1762 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala His 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1763 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1763 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaacgttt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1764 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1764 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Arg Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1765 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1765 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 cgtgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1766 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1766 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Arg Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1767 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1767 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1768 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1768 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asn Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1769 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1769 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ggggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1770 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1770 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1771 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1771 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaactta ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1772 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1772 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Leu Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1773 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1773 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgctttgtt tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1774 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1774 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Cys Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1775 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1775 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1776 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1776 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Val Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1777 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1777 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 aaagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1778 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1778 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Lys Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1779 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1779 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 tttgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta cccaagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1780 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1780 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Phe Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Lys 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1781 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1781 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaatata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1782 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1782 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Tyr Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1783 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1783 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1784 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1784 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1785 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1785 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaacgta ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1786 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1786 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Arg Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1787 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1787 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaattta ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1788 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1788 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Phe Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1789 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1789 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaaaa ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1790 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1790 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Lys Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1791 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1791 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaatata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1792 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1792 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Tyr Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1793 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1793 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 attgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1794 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1794 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Ile Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1795 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1795 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1796 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1796 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asn Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1797 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1797 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1798 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1798 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1799 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1799 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaaata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1800 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1800 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asn Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1801 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1801 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaacgta ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1802 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1802 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Arg Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1803 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1803 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 caagggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1804 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1804 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Gln Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1805 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1805 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccac gtggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1806 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1806 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Arg 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1807 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1807 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcattttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1808 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1808 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ile Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1809 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1809 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatgttt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1810 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1810 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Val Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1811 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1811 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatagtt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1812 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1812 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Ser Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1813 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1813 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaaagga ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1814 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1814 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Arg Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1815 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1815 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct ggaaatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1816 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1816 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Glu Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1817 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1817 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccac aaacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1818 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1818 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Thr Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1819 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1819 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgcag ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccca 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1820 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1820 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Ser Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Pro Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1821 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1821 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagctata gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1822 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1822 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Tyr Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1823 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1823 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcaaaa gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1824 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1824 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Lys Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1825 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1825 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccaaaga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1826 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1826 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Lys Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1827 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1827 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtcgt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1828 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1828 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Arg Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1829 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1829 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taaagcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcaac aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1830 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1830 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Lys Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Thr Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1831 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1831 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattggtaac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1832 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1832 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Gly Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1833 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1833 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgcaaac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1834 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1834 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Ala Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1835 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1835 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtcgt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1836 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1836 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Arg Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1837 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1837 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattaataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1838 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1838 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asn Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1839 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1839 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgaaac agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1840 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1840 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Lys Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1841 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1841 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattcataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1842 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1842 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile His Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1843 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1843 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatgc aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1844 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1844 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Ala Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1845 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1845 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtaaa 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1846 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1846 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Lys Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1847 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1847 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccaggga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1848 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1848 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Arg Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1849 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1849 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattaggaac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1850 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1850 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Arg Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1851 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1851 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcgtcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1852 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1852 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg Arg Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1853 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1853 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcagt 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattcaaaac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1854 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1854 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Ser Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Gln Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1855 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1855 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtcgt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1856 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1856 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Arg Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1857 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1857 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattagtaac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1858 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1858 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Ser Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1859 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1859 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat ttggaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1860 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1860 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Trp 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1861 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1861 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcgaaagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1862 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1862 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Glu Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1863 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1863 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctga cagcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1864 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1864 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Thr Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1865 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1865 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcccattcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1866 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1866 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Pro Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1867 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1867 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt ctgtagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1868 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1868 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Cys Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1869 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1869 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgaattcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1870 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1870 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Glu Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1871 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1871 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccatgggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1872 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1872 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Met Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1873 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1873 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctga cagcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1874 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1874 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Thr Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1875 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1875 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctga aagcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1876 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1876 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Lys Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1877 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1877 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgcaggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg tcgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1878 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1878 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Val Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1879 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1879 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 cattctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1880 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1880 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg His Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1881 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1881 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggaaaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1882 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1882 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Lys Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1883 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1883 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc cacccttggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1884 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1884 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Leu Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1885 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1885 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga agttgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1886 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1886 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Val Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1887 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1887 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aatggataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1888 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1888 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Met Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1889 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1889 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcaaattcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1890 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1890 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Lys Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1891 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1891 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctga cagcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1892 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1892 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Thr Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1893 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1893 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tccgtttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1894 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1894 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Arg Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1895 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1895 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgttggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1896 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1896 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Val Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1897 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1897 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggcgta ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcaccagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1898 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1898 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Arg Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Thr Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1899 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1899 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcagtagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1900 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1900 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Ser Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760 <210> 1901 <211> 2295 <212> DNA <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1901 agcaatatgt gggtgattgg caaaaacaaa gcccaggatg cgaaggccat tatggtcaat 60 gggccgcagt ttggttggta tgtgccggcg tacacctacg gtatcggcct gcacggcgcg 120 ggctatgacg tcaccggcaa tacgccgttt gcctatccgg gcctcgtttt tggtcacaac 180 ggcaccattt catggggatc caccgccggt gggggtgatg atgtcgatat ctttgccgaa 240 aaactttccg ccgagaagcc gggctattac cagcataacg gcgagtgggt gaagatgttg 300 agccgcaagg agactattgc ggtcaaagac ggccagccgg agacctttac cgtttggcgc 360 acgctgcacg gcaacgtcat taaaaccgat actgcgacgc agaccgccta tgccaaagcg 420 cgcgcctggg atggcaaaga ggtggcgtcc ctgctggcgt ggacgcacca gatgaaggcc 480 aaaaactggc cggagtggac gcagcaggcg gccaaacagg cgctgaccat taactggtac 540 tacgccgatg tgaacggcaa tatcggctat gtgcataccg gcgcctatcc ggatcgccag 600 cccggccacg acccgcgttt gccggttccc ggcactggaa aatgggactg gaaagggttg 660 ctgtcgtttg atttgaatcc gaaagtgtat aacccgcagt cgggctatat cgccaactgg 720 aacaactcgc cgcaaaaaga ctacccggcc tctgatctgt tcgcgttcct gtggggcggt 780 gcggatcgag ttactgagat cgacacgatc ctcgataagc aaccgcgctt caccgccgat 840 caggcgtggg atgtgatccg ccaaaccagc cgtcgggatc tcaacctgcg gttgttctta 900 ccggcgctga aggacgccac cgcgaacctg gcggaaaacg atccgcgccg ccaactggtg 960 gataaactgg cgagctggga cggcgaaaac cttgtcaacg atgacggaaa aacctatcag 1020 caaccgggat cggcgattct taacgcctgg ctgaccagca tgctcaagcg cacggtggtt 1080 gccgcggtcc cagcgccgtt tggtaagtgg tacagcgcca gtggctatga aaccacccag 1140 gacgggccaa ccggctcgct gaacatcagc gtgggggcga aaatcctcta cgaagctctg 1200 cagggtgata agtcgccaat cccgcaggcg gtcgatctgt ttggcgggaa accgcagcag 1260 gaagtaatac tggcggcgct ggacgacgct tggcagacgc tgtcaaaacg ctacggtaac 1320 gacgtcaccg gctggaaaac ccctgccatg gcgcttacct tccgggccaa taacttcttc 1380 ggcgtgccgc aggcggcagc aaaagaggcg cgtcatcagg cggagtacca gaaccgcggt 1440 acggaaaacg acatgattgt cttctcaccg acgtcgggta accgcccggt tcttgcctgg 1500 gatgtggtgg cgccggggca aagcggtttt atcgcgccgg atggcaaagc cgataagcac 1560 tatgacgatc agctgaaaat gtacgagagc tttggccgta aatcgctgtg gttaacgcct 1620 caggacgttg acgagcacca agagtctcag gaagtgctgc aggtacagtt ggatcagacc 1680 gaggttaaga tcgttcgcga tgaatacggc atgccgcata tttacgccga tgatacctat 1740 cgactgtttt acggctatgg ctacgtggtg gcgcaggatc gcctgttcca gatggaaatg 1800 gcgcgccgca gtactcaggg gaccgtctcc gaggtgctgg gcaaagcatt cgtcagtttt 1860 gataaagata ttcgccagaa ctactggccg gattctattc gcgcgcagat agcttccctc 1920 tccgctgagg ataaatccat tctgcagggc tatgccgatg gcatgaatgc gtggatcgat 1980 aaagtgaacg ccagccccga taagctgtta ccccagcagt tctccacctt tggttttaaa 2040 cccaagcatt gggaaccgtt tgatgtggcg atgatttttg tcggcaccat ggcgaaccgt 2100 ttctctgaca gcagtagcga aattgataac ctggcgctgc tgacggcgct aaaagacaaa 2160 tacggcaagc agcagggcat ggcggtcttt aaccagctga aatggctggt taatccttcc 2220 gcgccaacca ccattgcggc gcgggaaagc gcctatccgc tgaagtttga tctgcaaaac 2280 acgcaaacgg cgtaa 2295 <210> 1902 <211> 764 <212> PRT <213> Artificial Sequence <220> <223> Penicillin-G Acylates Variant from K. citrophila <400> 1902 Ser Asn Met Trp Val Ile Gly Lys Asn Lys Ala Gln Asp Ala Lys Ala 1 5 10 15 Ile Met Val Asn Gly Pro Gln Phe Gly Trp Tyr Val Pro Ala Tyr Thr 20 25 30 Tyr Gly Ile Gly Leu His Gly Ala Gly Tyr Asp Val Thr Gly Asn Thr 35 40 45 Pro Phe Ala Tyr Pro Gly Leu Val Phe Gly His Asn Gly Thr Ile Ser 50 55 60 Trp Gly Ser Thr Ala Gly Gly Gly Asp Asp Val Asp Ile Phe Ala Glu 65 70 75 80 Lys Leu Ser Ala Glu Lys Pro Gly Tyr Tyr Gln His Asn Gly Glu Trp 85 90 95 Val Lys Met Leu Ser Arg Lys Glu Thr Ile Ala Val Lys Asp Gly Gln 100 105 110 Pro Glu Thr Phe Thr Val Trp Arg Thr Leu His Gly Asn Val Ile Lys 115 120 125 Thr Asp Thr Ala Thr Gln Thr Ala Tyr Ala Lys Ala Arg Ala Trp Asp 130 135 140 Gly Lys Glu Val Ala Ser Leu Leu Ala Trp Thr His Gln Met Lys Ala 145 150 155 160 Lys Asn Trp Pro Glu Trp Thr Gln Gln Ala Ala Lys Gln Ala Leu Thr 165 170 175 Ile Asn Trp Tyr Tyr Ala Asp Val Asn Gly Asn Ile Gly Tyr Val His 180 185 190 Thr Gly Ala Tyr Pro Asp Arg Gln Pro Gly His Asp Pro Arg Leu Pro 195 200 205 Val Pro Gly Thr Gly Lys Trp Asp Trp Lys Gly Leu Leu Ser Phe Asp 210 215 220 Leu Asn Pro Lys Val Tyr Asn Pro Gln Ser Gly Tyr Ile Ala Asn Trp 225 230 235 240 Asn Asn Ser Pro Gln Lys Asp Tyr Pro Ala Ser Asp Leu Phe Ala Phe 245 250 255 Leu Trp Gly Gly Ala Asp Arg Val Thr Glu Ile Asp Thr Ile Leu Asp 260 265 270 Lys Gln Pro Arg Phe Thr Ala Asp Gln Ala Trp Asp Val Ile Arg Gln 275 280 285 Thr Ser Arg Arg Asp Leu Asn Leu Arg Leu Phe Leu Pro Ala Leu Lys 290 295 300 Asp Ala Thr Ala Asn Leu Ala Glu Asn Asp Pro Arg Arg Gln Leu Val 305 310 315 320 Asp Lys Leu Ala Ser Trp Asp Gly Glu Asn Leu Val Asn Asp Asp Gly 325 330 335 Lys Thr Tyr Gln Gln Pro Gly Ser Ala Ile Leu Asn Ala Trp Leu Thr 340 345 350 Ser Met Leu Lys Arg Thr Val Val Ala Ala Val Pro Ala Pro Phe Gly 355 360 365 Lys Trp Tyr Ser Ala Ser Gly Tyr Glu Thr Thr Gln Asp Gly Pro Thr 370 375 380 Gly Ser Leu Asn Ile Ser Val Gly Ala Lys Ile Leu Tyr Glu Ala Leu 385 390 395 400 Gln Gly Asp Lys Ser Pro Ile Pro Gln Ala Val Asp Leu Phe Gly Gly 405 410 415 Lys Pro Gln Gln Glu Val Ile Leu Ala Ala Leu Asp Asp Ala Trp Gln 420 425 430 Thr Leu Ser Lys Arg Tyr Gly Asn Asp Val Thr Gly Trp Lys Thr Pro 435 440 445 Ala Met Ala Leu Thr Phe Arg Ala Asn Asn Phe Phe Gly Val Pro Gln 450 455 460 Ala Ala Ala Lys Glu Ala Arg His Gln Ala Glu Tyr Gln Asn Arg Gly 465 470 475 480 Thr Glu Asn Asp Met Ile Val Phe Ser Pro Thr Ser Gly Asn Arg Pro 485 490 495 Val Leu Ala Trp Asp Val Val Ala Pro Gly Gln Ser Gly Phe Ile Ala 500 505 510 Pro Asp Gly Lys Ala Asp Lys His Tyr Asp Asp Gln Leu Lys Met Tyr 515 520 525 Glu Ser Phe Gly Arg Lys Ser Leu Trp Leu Thr Pro Gln Asp Val Asp 530 535 540 Glu His Gln Glu Ser Gln Glu Val Leu Gln Val Gln Leu Asp Gln Thr 545 550 555 560 Glu Val Lys Ile Val Arg Asp Glu Tyr Gly Met Pro His Ile Tyr Ala 565 570 575 Asp Asp Thr Tyr Arg Leu Phe Tyr Gly Tyr Gly Tyr Val Val Ala Gln 580 585 590 Asp Arg Leu Phe Gln Met Glu Met Ala Arg Arg Ser Thr Gln Gly Thr 595 600 605 Val Ser Glu Val Leu Gly Lys Ala Phe Val Ser Phe Asp Lys Asp Ile 610 615 620 Arg Gln Asn Tyr Trp Pro Asp Ser Ile Arg Ala Gln Ile Ala Ser Leu 625 630 635 640 Ser Ala Glu Asp Lys Ser Ile Leu Gln Gly Tyr Ala Asp Gly Met Asn 645 650 655 Ala Trp Ile Asp Lys Val Asn Ala Ser Pro Asp Lys Leu Leu Pro Gln 660 665 670 Gln Phe Ser Thr Phe Gly Phe Lys Pro Lys His Trp Glu Pro Phe Asp 675 680 685 Val Ala Met Ile Phe Val Gly Thr Met Ala Asn Arg Phe Ser Asp Ser 690 695 700 Ser Ser Glu Ile Asp Asn Leu Ala Leu Leu Thr Ala Leu Lys Asp Lys 705 710 715 720 Tyr Gly Lys Gln Gln Gly Met Ala Val Phe Asn Gln Leu Lys Trp Leu 725 730 735 Val Asn Pro Ser Ala Pro Thr Thr Ile Ala Ala Arg Glu Ser Ala Tyr 740 745 750 Pro Leu Lys Phe Asp Leu Gln Asn Thr Gln Thr Ala 755 760

Claims (49)

1. 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 1288과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 폴리펩티드 서열을 가지는 조작된 페니실린 G 아실라제.
2. 제1항에 있어서, 상기 페니실린 G 아실라제는 서열 번호: 4, 8, 14, 300, 1036, 1194, 1262, 및/또는 12884를 포함하는 것인 조작된 페니실린 G 아실라제.
3. 제1항에 있어서, 상기 페니실린 G 아실라제는 표 5.1, 6.1, 7.1, 8.1, 8.2, 9,1, 10.1, 12.1, 12.2, 12.3, 12.4, 12.5, 12.6, 및/또는 12.7에 기재된 적어도 하나의 서열과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 폴리펩티드 서열을 포함하는 것인 조작된 페니실린 G 아실라제.
4. 제1항에 있어서, 상기 페니실린 G 아실라제는 표 5.1, 6.1, 7.1, 8.1, 8.2, 9,1, 10.1, 12.1, 12.2, 12.3, 12.4, 12.5, 12.6, 및/또는 12.7에 기재된 폴리펩티드 서열을 포함하는 것인 조작된 페니실린 G 아실라제.
5. 제1항에 있어서, 상기 페니실린 G 아실라제는, 서열 번호: 8과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 27, 27;28;71;74;547, 27;71;74;484;547;584;697, 71;74, 129, 253, 254, 256, 348, 352, 372, 373, 374, 380, 380;457, 386;390, 386;390, 386, 387;390, 451, 457, 467, 470, 474, 616, 623, 704, 706, 및 708로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 8을 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제.
6. 제1항에 있어서, 상기 페니실린 G 아실라제는, 서열 번호: 14와 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 9, 9;103;119;131;233;312;324;432;444;494, 9;103;119;131;324;432;494;646, 9;103;119;131;233;269;304;444;494;646, 9;103;119;131;304;324;432;444;494;646, 9;103;119;233;646, 9;103;119;494, 9;103;233;312;646, 9;103;233;432;646, 9;103;233;494, 9;103;269;304;324;494;646, 9;103;304;432;444;646, 9;119;131;233, 9;119;131;233;304;444;646, 9;119;131;233;494;646, 9;119;131;233;494;661, 9;119;131;312;444;646, 9;119;131;432;444;646, 9;119;233;269;273;304;312;432;444;646, 9;119;233;494;646, 9;119;304;444;494;646, 9;131;233;269;273;312;432;444;646, 9;233;273;304;494;646, 9;233;304;494;646, 9;233;312;432;646, 9;233;494;646, 9;312;444;646, 9;432;444;494;646, 9;494;646, 28;374;380, 103;119;131;233;273;304;324;432;444;494;646, 103;119;131;233;304;312;432;494;646;661, 103;119;131;269;312;494;646, 103;119;233, 103;119;233;273;432, 103;119;233;304;646, 103;119;233;312;646, 103;119;494;646;661, 103;119;646, 103;131;233;304;324;444;646, 103;131;269;273;444;646, 103;233;273;312;324;432;444;646, 103;233;273;312;432;444;646, 103;269;273;444;646, 103;273;304;324;444;494, 103;312;444;646, 103;444;494;646, 119;131;444;646;661, 119;131;494, 119;131;646, 119;131;304;432;444;646;661, 119;131;444, 119;233;304;312;324;432;444;646, 119;233;304;646, 119;233;312, 119;233;646, 119;269;273;312;324;494;646, 119;269;273;312;432;444;646, 119;273;324;444;494;646, 119;312;444;646, 119;432;444;646, 129;254;348;457;704, 129;348, 129;348;467;470;704;708, 129;348;470;623;704;706, 129;348;470;623;704;706;708, 129;380;470, 129;457;470;474, 129;470,129;623, 131;233;273;646, 131;233;304;444, 131;233;432;646, 131;273;432;444;494;646, 185, 233;269;304;312;324;432;444;646, 253, 253;256, 253;256;352;373;374;616, 253;256;352;374;380;451, 253;256;374;451, 253;256;380;451;616, 253;352;374;616, 253;352;451;616, 253;373;451, 253;374;451, 253;374;451;623, 253;451, 253;451;457, 253;254;352;374;380, 253;256, 253;256;352;374;451, 253;256;352;380;451;616, 253;256;352;451, 253;256;352;374;380;451, 253;256;352;380, 253;256;373, 253;256;374;616, 253;256;380, 253;256;380;451;546;616, 253;352;373;374;451, 253;352;373;374;451;616, 253;352;374;616, 253;352;623, 253;373;374;451;616, 253;380, 254;255;352, 254;256;352;451, 254;256;373;374;380;451, 254;256;374, 254;256;374;451, 254;256;451, 254;352;380, 254;256;380;451;616, 254;352;380;451, 254;352;451, 254;373;374;380;451, 254;373;374;451, 254;374, 254;374;380, 254;380, 254;451, 254;616, 256;352;374, 256;352;380;451;616, 256;374;380;451;616, 256;374;616, 273;312;444;646, 304;312;444;646, 312;444;646, 312;646, 348, 348;372;470;623;708, 348;704;708, 352, 352;373;374;451, 352;373;380;451, 352;373;380;451;616, 352;373;451, 352;374, 352;374;380, 352;374;380;451;616, 352;374;451, 352;374;616, 352;380, 352;380;451, 352;380;451;616, 352;380;451;623, 352;380;616, 352;451, 352;451;616, 352;616, 372;457;470;623, 373;374, 373;374;451, 373;451, 373;616, 374, 374;380, 374;380;451, 374;380;451;623, 374;451, 374;451;616, 374;616, 374;623, 380, 380;451, 380;451;616, 380;451;623, 380;616, 380;616;623, 380;623, 415, 443, 444, 444;494, 444;646, 451, 451;616, 451;623, 457, 457;470, 457;704;708, 470;708, 492;493, 517, 560, 616, 623, 723, 및 748로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 14를 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제.
7. 제1항에 있어서, 상기 페니실린 G 아실라제는, 서열 번호: 300과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 9;61;444, 9;168;185;517;560;748, 9;185;415, 9;185;415;443;444;517;723;748, 9;185;415;443;444;517;560, 9;185;415;443;444;517;748, 9;185;415;444;517;723;748, 9;185;415;444;517;748, 9;185;415;444;517;560, 9;185;415;444;517;560;723;748, 9;185;415;444, 9;185;415;444;560, 9;185;415;444;723;748, 9;185;415;444;517;560;723;748, 9;185;415;517;560, 9;185;415;517;723, 9;185;415;517;748, 9;185;415;748, 9;185;443;444;517;560, 9;185;443;444;723, 9;185;443;444;560;723, 9;185;443;444;517, 9;185;444, 9;185;444;517;560, 9;185;444;560;723, 9;185;444;560;748, 9;185;444;517;560, 9;185;444;517;560;723, 9;185;444;517;560;748, 9;185;444;517;723;748, 9;185;444;560, 9;185;444;723, 9;185;444;517;560;723, 9;185;517, 9;185;517;560;723;748, 9;185;748, 9;415;443;444;517;560, 9;415;443;444;517;748, 9;415;443;444;560;723;748, 9;415;443;444;517;560, 9;415;443;444;517;560;723, 9;415;443;444;517;748, 9;415;443;560, 9;415;443;560;723;748, 9;415;444, 9;415;444;517;560;748, 9;415;444;517, 9;415;444;517;560;723;748, 9;415;444;560;723;748, 9;415;444;723;748, 9;415;444;517, 9;415;444;517;560;723;748, 9;415;444;560;723, 9;415;444;517, 9;415;444;517;560;748, 9;415;444;517, 9;415;444;560, 9;415;444;560, 9;415;444;517;560;723, 9;415;444;560, 9;415;444;560;665;723;748, 9;415;444;723;748, 9;415;517, 9;415;517;560;723;748, 9;415;517;560;723;748, 9;415;517;560;748, 9;415;560, 9;415;748, 9;443;444;517;560;723, 9;443;444;560, 9;443;444;560;723;748, 9;443;444;517, 9;443;444;560;723, 9;443;444;517, 9;443;444;517;560, 9;443;444;517;748, 9;443;517;748, 9;443;723, 9;444;517;560, 9;444;560;723, 9;444;748, 9;444;517;560;723;748, 9;444;560, 9;444;560;723, 9;444;560;748, 9;444;560;748, 9;444;748, 9;444;517;560;723;748, 9;444;517, 9;444;517;560;723, 9;444;517;723, 9;444;560;748, 9;444;723, 9;444, 9;444;517;560, 9;517, 9;517;560;723, 9;517;560;748, 9;517;560;748, 9;517;723, 9;517;748, 9;560;723;748, 9;723, 103, 103;119, 103;119;129;254;256;348;494;646, 103;119;129;444;494, 103;119;254;348,103;119;254;348;444, 103;119;254;444, 103;119;256;348;444;494;646, 103;119;256;494, 103;119;348, 103;119;348;457, 103;119;348;457;494, 103;119;457, 103;119;494, 103;119;494;646, 103;129, 103;129;254;444;457;494, 103;129;256;348, 103;129;348;646, 103;254, 103;254;256;348;444;494, 103;254;646,103;254;348, 103;254;348;494, 103;256, 103;256;444, 103;256;457, 103;256;494, 103;348, 103;348;444, 103;348;494, 103;444, 103;494, 103;494;646,119;129;254;348;494, 119;129;254;457;494, 119;129;256;348;457, 119;254;348;457, 119;256;348 119;256;348;494, 119;256;444, 119;348, 119;348;494, 185;415;443;444;517;560, 185;415;443;444;517, 185;415;444;517;560;748, 185;415;444;517;748, 185;415;444;560, 185;415;560, 185;415;560;723, 185;415;723;748, 185;443;444;560, 185;444;446;517;560, 185;444;517;560, 185;444;517;560;723;748, 185;444;517;723, 185;444;560, 185;517;560;723,185;560, 185;560;723, 185;560;748, 254;457, 256;348, 256;494, 348, 348;444, 348;444;646, 348;457, 348;494, 415, 415;443;444;517;748, 415;443;444;560, 415;443;517;560;723, 415;443;517;723, 415;444;723, 415;444;517, 415;444;517;560;723;748, 415;444;517;560;748, 415;444;560;723, 415;444;517;560;723, 415;444;517;723;748 415;517;560;723;748, 415;444;560, 415;444;560;748, 415;444, 415;517, 415;517;560, 415;517;560;748, 415;560;723, 415;723;748, 415;723;748, 415;748, 443;444;517;560;723;748, 443;444;517;748, 443;444, 443;444;560, 443;444;723, 443;517, 444, 444;517;560, 444;517;748, 444;748, 444;517;560;723;748, 444;560;723, 444;560, 444;560;723, 444;517, 444;517;560, 444;517;723;748, 444;560;748, 444;723, 444;517;560, 444;560;723, 517;560, 517;560;748, 517;723, 517;748, 517;748, 560, 723, 및 723;748로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 300을 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제.
8. 제1항에 있어서, 상기 페니실린 G 아실라제는, 서열 번호: 1262와 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 24, 24;27;28;701;729, 24;28;56;308;379;701, 24;28;56;701, 24;28;71;701, 24;28;321;701, 24;28;457;701, 24;31;56;386;701, 24;31, 24;31;56;697, 24;31;56;701, 24;31;56;264;701;750, 24;31;71;701, 24;56;154;270;697, 24;56;697;701, 24;56;701, 24;71;701, 24;225;701, 24;484;701, 24;28, 28;31, 31;56;701, 56;71;701, 56;119;146;701, 56;154;701, 56;322;697;701, 56;658;701, 56;697;701, 56;701,56;701;711, 697, 697;701, 71;74, 71;701, 129;511;701, 154;754, 177, 410;697;701, 423;701, 431, 697, 및 701로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1262를 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제.
9. 제1항에 있어서, 상기 페니실린 G 아실라제는, 서열 번호: 1288과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 22, 31, 31;56;264;308;379;484;547;711;750, 32, 50, 57, 69;74, 71, 71;74, 71;74;129, 71;74;145, 71;74;248, 71;74;470, 71;149, 75, 141, 및 394로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1288을 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제.
10. 제1항에 있어서, 상기 페니실린 G 아실라제는, 서열 번호: 1036과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 2, 47, 176, 253, 255, 384, 460, 467, 536, 및 623으로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1036을 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제.
11. 제1항에 있어서, 상기 페니실린 G 아실라제는, 서열 번호: 1194와 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 12;103;119;131;233;384;444;494;S646, 12;103;119;131;233;444;494;S646, 12;103;119;233;384;444;494;S646, 12;103;119;233;444;467;494;S646, 12;103;119;233;444;494;536;S646, 12;103;119;233;444;494;S646, 12;103;131;233;444;467;494;S646, 12;103;131;233;444;494;S646, 12;103;233;444;494;S646, 12;119;233;384;444;494;S646, 28;264;384;467;484;536;547, 103;119;131;233;384;444;494;S646, 103;119;233;444;494;S646, 103;233;444;494;S646, 264;384;467;484;536;547, 384;467;484;536;547, 및 668로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1194를 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제.
12. 제1항에 있어서, 상기 페니실린 G 아실라제는, 서열 번호: 1288과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 20;709, 27, 27;74;253;254, 27;74;253;254;255;348;369;370;381, 27;74;253;254;255;348;370;384, 27;74;253;254;255;369;370, 27;74;253;254;255;370, 27;74;253;254;255;370;381;384, 27;74;253;254;255;381, 27;74;253;254;348, 27;74;253;254;384, 27;74;253;255, 27;74;253;255;348;370;384, 27;74;253;255;348;381, 27;74;253;255;348;384, 27;74;253;348;369;370, 27;74;253;348;369;370;381, 27;74;253;348;369;370;381;384, 27;74;253;348;370, 27;74;253;381;384, 27;74;253;384, 27;74;254;255;348, 27;74;254;255;348;369;370;381, 27;74;254;255;348;370, 27;74;254;255;348;381, 27;74;254;255;381, 27;74;254;348;381;384, 27;74;254;369;370, 27;74;255;348, 27;74;255;348;369;370, 27;74;255;348;369;381;384, 27;74;255;370, 27;74;348, 27;74;369;370, 27;74;107;255;348;369;370, 27;74;253, 27;74;253;254;255, 27;74;253;254;255;348;370, 27;74;253;254;348;369;370;381, 27;74;253;254;348;369;384, 27;74;253;254;348;370, 27;74;253;254;348;370;381, 27;74;253;254;369, 27;74;253;254;370;381, 27;74;253;255;348;369;370, 27;74;253;255;370, 27;74;253;348, 27;74;253;348;370;381;384, 27;74;253;369;381;384, 27;74;254,27;74;254;255;348;369;381, 27;74;254;255;348;370;381, 27;74;254;255;369, 27;74;254;348, 27;74;254;348;369;381;384, 27;74;254;348;370, 27;74;254;348;370;381, 27;74;254;384, 27;74;255;348;370, 27;74;348;384, 27;74;253;254;255;348;369;370, 27;74;253;254;348;369, 27;74;253;254;348;369;370, 27;74;253;254;348;370;381;384, 27;74;253;254;348;381;384, 27;74;253;370, 27;74;254;255;348;369;370, 27;74;254;255;348;381;384, 27;74;370, 27;74;253;254;255;348, 27;74;253;255;348;370;381, 27;74;253;255;384, 27;74;253;348;369;370;384, 27;74;253;348;381, 27;74;254;255;348;369;384, 27;74;254;255;348;370;381;384, 27;74;254;255;370, 27;74;254;348;381, 27;74;254;369;384, 27;74;255, 27;74;255;348;369;381, 27;74;348;370, 27;74;369;370;381;384, 27;253, 27;253;254, 27;253;254;255, 27;253;254;255;260;348;381;384, 27;253;254;255;348, 27;253;254;255;348;369;370;381;384, 27;253;254;255;348;369;384, 27;253;254;255;348;370, 27;253;254;255;348;370;384, 27;253;254;255;348;381;384, 27;253;254;348, 27;253;254;348;370;381, 27;253;254;348;370;384, 27;253;254;348;381, 27;253;254;348;381;384, 27;253;254;348;384, 27;253;254;381, 27;253;254;381;384, 27;253;254;384, 27;253;255;348, 27;253;255;348;369;370, 27;253;255;348;381, 27;253;255;348;384, 27;253;255;370, 27;253;255;370;381;384, 27;253;348, 27;253;348;370;381;384, 27;253;348;370;384, 27;253;348;381;384, 27;253;369;370, 27;253;381;384, 27;254;255, 27;254;255;348, 27;254;255;348;369;370, 27;254;255;348;370, 27;254;255;348;370;381, 27;254;255;348;370;381;384, 27;254;255;348;370;384, 27;254;255;369;370;381;384, 27;254;255;370, 27;254;255;381;384, 27;254;255;384, 27;254;348;369;370;381;384, 27;254;348;370, 27;254;348;381;384, 27;254;348;384, 27;254;369;381;384, 27;254;449, 27;254;470, 27;255;348, 27;255;348;370, 27;255;348;370;381;384, 27;255;348;381;384, 27;255;370, 27;348, 27;348;369;370;381;384, 27;348;381, 27;348;384, 69, 74;253;254;369;370, 74;254;255;348;384, 84, 128, 131, 132, 133, 134, 253, 253;348;370, 254, 255, 255;348;370, 256, 317;380, 348;467, 370, 373, 377, 381, 381;672, 383, 384, 388, 453, 457, 467, 472, 615, 616, 618, 619, 620, 623, 627, 701, 705, 706, 708, 및 709로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1288을 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제.
13. 제1항에 있어서, 상기 페니실린 G 아실라제는, 서열 번호: 1262와 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일하고, 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 24;31;56;701, 24;31;56;701, 24;31;71;701, 24;56;701, 및 71;74로부터 선택되는 치환 세트를 포함하는 폴리펩티드 서열을 포함하며, 상기 위치는 서열 번호: 1262를 참조로 하여 넘버링된 것인 조작된 페니실린 G 아실라제.
14. 제1항 내지 제14항 중 어느 한 항에 있어서, 상기 페니실린 G 아실라제는 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 생산할 수 있는 것인 조작된 페니실린 G 아실라제.
15. 제1항에 있어서, 상기 페니실린 G 아실라제는 서열 번호: 4-1902 중 짝수 번호의 서열에 제공된 적어도 하나의 서열과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 폴리펩티드 서열을 포함하는 것인 조작된 페니실린 G 아실라제.
16. 제1항에 있어서, 상기 페니실린 G 아실라제는 서열 번호: 3, 7, 13, 299, 1035, 1193, 1261, 및/또는 1287과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 폴리뉴클레오티드 서열에 의해 코딩되는 것인 조작된 페니실린 G 아실라제.
17. 제1항 내지 제16항 중 어느 한 항의 조작된 페니실린 G 아실라제를 코딩하는 조작된 폴리뉴클레오티드 서열.
18. 조작된 페니실린 G 아실라제를 코딩하는 조작된 폴리뉴클레오티드 서열로서, 상기 조작된 폴리뉴클레오티드 서열은 서열 번호: 3-1901 중 홀수 번호의 서열에 제공된 적어도 하나의 서열과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 것인 조작된 폴리뉴클레오티드 서열.
19. 서열 번호: 3, 7, 13, 299, 1035, 1193, 1261, 및/또는 1287과 적어도 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 이상 동일한 폴리펩티드 서열을 포함하는 조작된 페니실린 G 아실라제를 코딩하는 조작된 폴리뉴클레오티드 서열.
20. 제17항 내지 제19항 중 어느 한 항의 폴리뉴클레오티드 서열을 포함하는 벡터.
21. 제11항의 벡터를 포함하는 숙주 세포.
22. 제12항에 있어서, 상기 숙주 세포는 원핵생물 또는 진핵생물 세포인 숙주 세포.
23. 제13항에 있어서, 상기 원핵생물 숙주 세포는 이. 콜라이(E. coli)인 숙주 세포.
24. 제1항 내지 제16항 중 어느 한 항에 제공된 적어도 하나의 조작된 페니실린 G 아실라제를 포함하는 조성물.
25. 제22항 또는 제23항의 숙주 세포를 조작된 페니실린 G 아실라제가 생산되는 조건하에서 배양하는 단계를 포함하는, 제1항 내지 제16항 중 어느 한 항의 조작된 페니실린 G 아실라제를 제조하는 방법.
26. i) 제1항 내지 제16항 중 어느 한 항의 조작된 페니실린 G 아실라제 및/또는 제24항의 조성물, 및 A1/B1/B29 트리-페닐 아세테이트 보호기를 포함하는 인슐린을 제공하는 단계; 및 ii) 상기 조작된 페니실린 G 아실라제가 상기 인슐린으로부터 A1, B1 및/또는 B29 트리-페닐 아세테이트 보호기를 제거하여 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 제조하는 조건하에서 상기 조작된 페니실린 G 아실라제를 A1/B1/B29 트리-페닐 아세테이트 보호기를 포함하는 상기 인슐린에 노출시키는 단계를 포함하는, 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 제조하는 방법.
27. 제26항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 A1 트리-페닐 아세테이트 보호기를 제거하는 것인 방법.
28. 제26항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 B1 트리-페닐 아세테이트 보호기를 제거하는 것인 방법.
29. 제26항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 B29 트리-페닐 아세테이트 보호기를 제거하는 것인 방법.
30. 제26항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 A1, B1, 및 B29 트리-페닐 아세테이트 보호기를 제거하는 것인 방법.
31. 제26항 내지 제29항 중 어느 한 항에 있어서, 상기 조작된 페니실린 G 아실라제는 서열 번호: 2의 폴리펩티드에 의한 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린의 생산과 비교하였을 때, 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 초과 또는 그 이상으로 생산하는 것인 방법.
32. 제26항 내지 제31항 중 어느 한 항의 방법에 따라 제조된 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 포함하는 조성물.
33. i) 제1항 내지 제16항 중 어느 한 항의 조작된 페니실린 G 아실라제 및/또는 제24항의 조성물, 및 유리 인슐린을 제공하는 단계; 및 ii) 상기 조작된 페니실린 G 아실라제가 A1, B1, 및/또는 B29 위치를 아실화시켜 단일 보호된 또는 이중 보호된 인슐린을 제조하는 조건하에서 상기 조작된 페니실린 G 아실라제를 상기 인슐린에 노출시키는 단계를 포함하는, 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 제조하는 방법.
34. 제33항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 A1 위치를 아실화시키는 것인 방법.
35. 제33항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 B1 위치를 아실화시키는 것인 방법.
36. 제33항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 B29 위치를 아실화시키는 것인 방법.
37. 제33항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 A1, B1, 및 B29 위치를 아실화시키는 것인 방법.
38. 제32항에 있어서, 상기 조작된 페니실린 G 아실라제는 서열 번호: 2의 폴리펩티드에 의한 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린의 생산과 비교하였을 때, 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 초과 또는 그 이상으로 생산하는 것인 방법.
39. 제33항 내지 제38항 중 어느 한 항의 방법에 따라 제조된 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 포함하는 조성물.
40. i) 제1항 내지 제16항 중 어느 한 항의 조작된 페니실린 G 아실라제 및/또는 제24항의 조성물, 및 인슐린을 제공하는 단계; 및 ii) 상기 조작된 페니실린 G 아실라제가 A1, B1 및/또는 B29 트리-페닐 아세테이트 보호기를 상기 인슐린에 부가하여 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 제조하는 조건하에서 상기 조작된 페니실린 G 아실라제를 상기 인슐린에 노출시키는 단계를 포함하는, 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 제조하는 방법.
41. 제40항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 A1 트리-페닐 아세테이트 보호기를 부가하는 것인 방법.
42. 제40항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 B1 트리-페닐 아세테이트 보호기를 부가하는 것인 방법.
43. 제40항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 B29 트리-페닐 아세테이트 보호기를 부가하는 것인 방법.
44. 제40항에 있어서, 상기 페니실린 G 아실라제는 상기 인슐린의 A1, B1, 및 B29 트리-페닐 아세테이트 보호기를 부가하는 것인 방법.
45. 제40항 내지 제44항 중 어느 한 항에 있어서, 상기 조작된 페니실린 G 아실라제는 서열 번호: 2의 폴리펩티드에 의한 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린의 생산과 비교하였을 때, 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 초과 또는 그 이상으로 생산하는 것인 방법.
46. 제40항 내지 제45항 중 어느 한 항의 방법에 따라 제조된 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 포함하는 조성물.
47. 제26항 내지 제31항, 제33항 내지 제38항 및 제40항 내지 제45항 중 어느 한 항에 있어서, 상기 페니실린 G 아실라제는 서열 번호: 837 또는 1219를 포함하는 것인 방법.
48. 제26항 내지 제31항, 제33항 내지 제38항 및 제40항 내지 제45항 중 어느 한 항에 있어서, 상기 페니실린 G 아실라제는 서열 번호: 897 또는 1859를 포함하는 것인 방법.
49. 제26항 내지 제31항, 제33항 내지 제38항 및 제40항 내지 제45항 중 어느 한 항의 적어도 하나의 방법에 따라 제조된 페닐 아세테이트 단일 보호된 또는 이중 보호된 인슐린을 포함하는 조성물.

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