JP2019510515A5 - - Google Patents
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- Publication number
- JP2019510515A5 JP2019510515A5 JP2019503197A JP2019503197A JP2019510515A5 JP 2019510515 A5 JP2019510515 A5 JP 2019510515A5 JP 2019503197 A JP2019503197 A JP 2019503197A JP 2019503197 A JP2019503197 A JP 2019503197A JP 2019510515 A5 JP2019510515 A5 JP 2019510515A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- oligonucleotide
- sequence
- item
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108020004707 nucleic acids Proteins 0.000 claims description 142
- 150000007523 nucleic acids Chemical class 0.000 claims description 142
- 229920000272 Oligonucleotide Polymers 0.000 claims description 119
- 230000003321 amplification Effects 0.000 claims description 97
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 97
- 230000000295 complement Effects 0.000 claims description 72
- 229920000023 polynucleotide Polymers 0.000 claims description 70
- 239000002157 polynucleotide Substances 0.000 claims description 70
- 230000000694 effects Effects 0.000 claims description 54
- 229920001850 Nucleic acid sequence Polymers 0.000 claims description 29
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims description 20
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 18
- 238000001514 detection method Methods 0.000 claims description 9
- 101710036216 ATEG_03556 Proteins 0.000 claims description 8
- 108010092799 EC 2.7.7.49 Proteins 0.000 claims description 8
- 101710003000 ORF1/ORF2 Proteins 0.000 claims description 8
- 101700030467 Pol Proteins 0.000 claims description 8
- 101710004466 rgy Proteins 0.000 claims description 8
- 101710030364 rgy1 Proteins 0.000 claims description 8
- 101710030359 rgy2 Proteins 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 101700008821 EXO Proteins 0.000 claims description 6
- 101700083023 EXRN Proteins 0.000 claims description 6
- 102000018120 Recombinases Human genes 0.000 claims description 6
- 108010091086 Recombinases Proteins 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 238000003776 cleavage reaction Methods 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000031063 Single-stranded DNA-binding protein Human genes 0.000 claims description 3
- 108091000122 Single-stranded DNA-binding protein Proteins 0.000 claims description 3
- 102000033147 ERVK-25 Human genes 0.000 claims 2
- 239000000523 sample Substances 0.000 description 80
- 102100005410 LINE-1 retrotransposable element ORF2 protein Human genes 0.000 description 8
- 230000002255 enzymatic Effects 0.000 description 4
- 229920000160 (ribonucleotides)n+m Polymers 0.000 description 2
- 108020004513 Bacterial RNA Proteins 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 108020000999 Viral RNA Proteins 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000012521 purified sample Substances 0.000 description 2
- 230000001809 detectable Effects 0.000 description 1
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2020092422A JP7426901B2 (ja) | 2016-04-04 | 2020-05-27 | 等温増幅の成分およびプロセス |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US15/090,405 US9617587B1 (en) | 2016-04-04 | 2016-04-04 | Isothermal amplification components and processes |
US15/090,405 | 2016-04-04 | ||
PCT/US2017/020921 WO2017176404A1 (en) | 2016-04-04 | 2017-03-06 | Isothermal amplification components and processes |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2020092422A Division JP7426901B2 (ja) | 2016-04-04 | 2020-05-27 | 等温増幅の成分およびプロセス |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2019510515A JP2019510515A (ja) | 2019-04-18 |
JP2019510515A5 true JP2019510515A5 (ru) | 2020-03-19 |
JP6758474B2 JP6758474B2 (ja) | 2020-09-23 |
Family
ID=58348016
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2019503197A Active JP6758474B2 (ja) | 2016-04-04 | 2017-03-06 | 等温増幅の成分およびプロセス |
JP2020092422A Active JP7426901B2 (ja) | 2016-04-04 | 2020-05-27 | 等温増幅の成分およびプロセス |
JP2024087154A Pending JP2024114703A (ja) | 2016-04-04 | 2024-05-29 | 等温増幅の成分およびプロセス |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2020092422A Active JP7426901B2 (ja) | 2016-04-04 | 2020-05-27 | 等温増幅の成分およびプロセス |
JP2024087154A Pending JP2024114703A (ja) | 2016-04-04 | 2024-05-29 | 等温増幅の成分およびプロセス |
Country Status (8)
Country | Link |
---|---|
US (4) | US9617587B1 (ru) |
EP (4) | EP4389910A3 (ru) |
JP (3) | JP6758474B2 (ru) |
CN (1) | CN109196114A (ru) |
AU (2) | AU2017247133B2 (ru) |
CA (1) | CA3018202A1 (ru) |
ES (2) | ES2940071T3 (ru) |
WO (1) | WO2017176404A1 (ru) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
KR101884793B1 (ko) * | 2017-11-23 | 2018-08-02 | 강원대학교산학협력단 | Cage 단백질의 생산 및 정제 방법 |
CN110558279B (zh) * | 2019-09-16 | 2021-11-16 | 南方科技大学 | 一种药物筛选模型、药物筛选方法及其应用 |
CN118647731A (zh) | 2022-02-05 | 2024-09-13 | 贝克顿迪金森公司 | 用于分离基因组dna以扩增短核酸靶的方法 |
WO2023150710A1 (en) | 2022-02-05 | 2023-08-10 | Becton, Dickinson And Company | Non-opaque lytic buffer composition formulations |
CN118786224A (zh) | 2022-02-14 | 2024-10-15 | 贝克顿迪金森公司 | 用于核酸扩增的内部对照 |
WO2024054823A1 (en) | 2022-09-07 | 2024-03-14 | Becton, Dickinson And Company | Hairpin internal control for isothermal nucleic acid amplification |
WO2024054867A1 (en) | 2022-09-07 | 2024-03-14 | Becton, Dickinson And Company | Modified molecular beacons for improved detection specificity |
WO2024054825A1 (en) * | 2022-09-07 | 2024-03-14 | Becton, Dickinson And Company | Archaeal polymerase amplification |
WO2024118922A1 (en) | 2022-12-01 | 2024-06-06 | Becton, Dickinson And Company | Asymmetric hairpin probes for nucleic acid detection |
Family Cites Families (56)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5693517A (en) | 1987-06-17 | 1997-12-02 | Roche Molecular Systems, Inc. | Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions |
US5705345A (en) | 1991-01-10 | 1998-01-06 | Amersham International Plc | Methods and kits for preparing nucleic acids using cyclodextrin |
CA2184270C (en) | 1994-03-10 | 1999-08-17 | Daniel Louis Kacian | Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents |
US20050089918A1 (en) | 1998-02-23 | 2005-04-28 | Amersham Biosciences Uk Limited | In-situ cell extraction and assay method |
US8268605B2 (en) * | 1999-10-29 | 2012-09-18 | Agilent Technologies, Inc. | Compositions and methods utilizing DNA polymerases |
MXPA02012739A (es) | 2001-03-09 | 2004-04-20 | Nugen Technologies Inc | Metodos y composiciones para amplificacion de secuencias de arn. |
WO2002101042A1 (fr) | 2001-06-12 | 2002-12-19 | Takara Bio Inc. | Procede de stabilisation d'un reactif pour amplifier ou detecter l'acide nucleique et procede de memorisation |
WO2003008624A2 (en) | 2001-07-15 | 2003-01-30 | Keck Graduate Institute | Nucleic acid amplification using nicking agents |
EP1992706B1 (en) | 2002-02-21 | 2014-11-19 | Alere San Diego, Inc. | Recombinase polymerase amplification |
WO2004021986A2 (en) | 2002-09-03 | 2004-03-18 | Quanta Biosciences, Inc. | Improved compositions and methods for cdna synthesis |
US7662594B2 (en) | 2002-09-20 | 2010-02-16 | New England Biolabs, Inc. | Helicase-dependent amplification of RNA |
EP2336361B1 (en) | 2004-06-01 | 2019-01-23 | Alere San Diego, Inc. | Kit for recombinase polymerase amplification |
EP2829615B1 (en) | 2005-07-25 | 2018-05-09 | Alere San Diego, Inc. | Kit for multiplexing recombinase polymerase amplification |
US8551697B1 (en) | 2005-12-09 | 2013-10-08 | Applied Biosystems, Llc | Electrochemical polynucleotide detection comprising ligation |
JP2009535053A (ja) | 2006-05-04 | 2009-10-01 | エーエスエム サイエンティフィック, インコーポレイテッド | レコンビナーゼポリメラーゼ増幅 |
JP4918409B2 (ja) | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
CA2673772A1 (en) | 2007-01-17 | 2008-07-24 | Meridian Bioscience, Inc. | Stable reagents and kits useful in loop-mediated isothermal amplification (lamp) |
CN103952470B (zh) * | 2007-03-28 | 2017-11-10 | 信号诊断公司 | 高解析度分析核酸以检测序列变异的系统和方法 |
US8008010B1 (en) * | 2007-06-27 | 2011-08-30 | Applied Biosystems, Llc | Chimeric oligonucleotides for ligation-enhanced nucleic acid detection, methods and compositions therefor |
US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
US8435741B2 (en) | 2007-07-26 | 2013-05-07 | Fujifilm Corporation | Isothermal nucleic acid amplification method using surfactant |
JP2009048280A (ja) | 2007-08-15 | 2009-03-05 | Nikon Corp | 機構解析プログラム |
KR20100063079A (ko) * | 2007-08-23 | 2010-06-10 | 노파르티스 아게 | 올리고뉴클레오티드의 탐지 방법 |
US20090162856A1 (en) | 2007-11-06 | 2009-06-25 | Hayato Miyoshi | Rna detection method |
US8338094B2 (en) | 2007-11-27 | 2012-12-25 | Bio-Rad Laboratories, Inc. | Reduced inhibition of one-step RT-PCR |
JP5616584B2 (ja) | 2009-01-09 | 2014-10-29 | 富士フイルム株式会社 | 核酸配列の複製方法 |
WO2010135310A1 (en) | 2009-05-20 | 2010-11-25 | Biosite Incorporated | Dna glycosylase/lyase and ap endonuclease substrates |
WO2010141940A1 (en) | 2009-06-05 | 2010-12-09 | Alere San Diego, Inc. | Recombinase polymerase amplification reagents and kits |
US20130059290A1 (en) | 2009-09-25 | 2013-03-07 | Alere San Diego, Inc. | Detection of nucleic acids in crude matrices |
EP2418286A1 (en) | 2010-08-10 | 2012-02-15 | QIAGEN GmbH | Improved method for isothermal amplification of nucleic acids |
TW201239086A (en) | 2011-03-23 | 2012-10-01 | Genereach Biotechnology Corp | Polymerase chain reaction solution |
JP5961247B2 (ja) | 2011-04-07 | 2016-08-02 | アリーア サン ディエゴ, インコーポレイテッド | リコンビナーゼポリメラーゼ増幅混合物のモニタリング |
US9957558B2 (en) | 2011-04-28 | 2018-05-01 | Life Technologies Corporation | Methods and compositions for multiplex PCR |
WO2012154934A1 (en) | 2011-05-11 | 2012-11-15 | New England Biolabs, Inc. | Dna polymerase variants with reduced exonuclease activity and uses thereof |
CA2881200A1 (en) | 2012-02-14 | 2013-08-22 | Great Basin Scientific | Methods of isothermal amplification using blocked primers |
JP5958034B2 (ja) | 2012-04-11 | 2016-07-27 | 富士レビオ株式会社 | 分子ビーコン型プローブを用いた標的核酸の検出方法 |
CA3096342C (en) | 2012-06-08 | 2023-04-04 | Ionian Technologies, Llc | Nucleic acid amplifications |
WO2014003583A2 (en) | 2012-06-26 | 2014-01-03 | University Of The Philippines Diliman | Detection of pathogens |
GB201220662D0 (en) | 2012-11-16 | 2013-01-02 | ALERE TECHNOLOGIES GmbH | Assays |
JP5904153B2 (ja) | 2013-03-29 | 2016-04-13 | ソニー株式会社 | 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ |
US10443094B2 (en) | 2014-03-26 | 2019-10-15 | General Electric Company | Solid phase isothermal amplification |
WO2016099999A1 (en) | 2014-12-18 | 2016-06-23 | Ge Healthcare Uk Limited | Analyte detection on a solid support by nucleic acid amplification coupled to an immunoassay |
US9868944B2 (en) | 2014-12-19 | 2018-01-16 | Roche Molecular Systems, Inc. | Reaction mixtures |
US10822645B1 (en) | 2014-12-23 | 2020-11-03 | Global Life Sciences Solutions Operations UK Ltd | Methods and reagents for reverse-transcription polymerase chain reaction |
GB201519565D0 (en) | 2015-11-05 | 2015-12-23 | Alere San Diego Inc | Sample preparation device |
EP3368654A4 (en) | 2015-10-30 | 2019-06-05 | Alere Inc. | DETERMINATION OF POLYMORPHISMS BY ISOTHERMAL AMPLIFICATION OF NUCLEIC ACID |
US10329601B2 (en) | 2015-12-28 | 2019-06-25 | Ionian Technologies, Inc. | Nicking and extension amplification reaction (NEAR) of Streptococcus species |
US9757728B2 (en) * | 2016-01-26 | 2017-09-12 | Lidong Qin | Microfluidic aliquoting for single-cell isolation |
GB201602880D0 (en) | 2016-02-18 | 2016-04-06 | Ge Healthcare Uk Ltd | Method and composition for biomolecule stabilization |
CN107119040A (zh) * | 2016-02-24 | 2017-09-01 | 青岛艾菲生物技术有限公司 | 一种等温核酸扩增的方法 |
WO2017152122A2 (en) | 2016-03-04 | 2017-09-08 | Alere San Diego Inc. | Automated nested recombinase polymerase amplification |
US11299777B2 (en) * | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
US11268117B2 (en) | 2016-06-10 | 2022-03-08 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
JP7308148B2 (ja) | 2017-01-30 | 2023-07-13 | ゾエティス サービシズ リミテッド ライアビリティ カンパニー | 溶液ベースのプラズモン特異的結合パートナーアッセイおよび金属ナノ構造体 |
EP3574047B1 (en) | 2017-01-30 | 2024-03-06 | Bio-Rad Laboratories, Inc. | Emulsion compositions and methods of their use |
-
2016
- 2016-04-04 US US15/090,405 patent/US9617587B1/en active Active
-
2017
- 2017-03-06 AU AU2017247133A patent/AU2017247133B2/en active Active
- 2017-03-06 WO PCT/US2017/020921 patent/WO2017176404A1/en active Application Filing
- 2017-03-06 EP EP24156601.7A patent/EP4389910A3/en active Pending
- 2017-03-06 JP JP2019503197A patent/JP6758474B2/ja active Active
- 2017-03-06 EP EP17711527.6A patent/EP3440217B1/en active Active
- 2017-03-06 EP EP22165594.7A patent/EP4053291B1/en active Active
- 2017-03-06 CN CN201780033123.7A patent/CN109196114A/zh active Pending
- 2017-03-06 ES ES20182776T patent/ES2940071T3/es active Active
- 2017-03-06 CA CA3018202A patent/CA3018202A1/en active Pending
- 2017-03-06 EP EP20182776.3A patent/EP3760735B1/en active Active
- 2017-03-06 ES ES17711527T patent/ES2881509T3/es active Active
-
2020
- 2020-05-27 JP JP2020092422A patent/JP7426901B2/ja active Active
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2021
- 2021-08-13 US US17/402,358 patent/US11884969B2/en active Active
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2022
- 2022-03-15 US US17/695,600 patent/US20220251629A1/en active Pending
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2023
- 2023-02-24 AU AU2023201140A patent/AU2023201140A1/en active Pending
- 2023-12-14 US US18/540,659 patent/US20240209426A1/en active Pending
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2024
- 2024-05-29 JP JP2024087154A patent/JP2024114703A/ja active Pending
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