JP2018527902A5 - - Google Patents
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- JP2018527902A5 JP2018527902A5 JP2018502120A JP2018502120A JP2018527902A5 JP 2018527902 A5 JP2018527902 A5 JP 2018527902A5 JP 2018502120 A JP2018502120 A JP 2018502120A JP 2018502120 A JP2018502120 A JP 2018502120A JP 2018527902 A5 JP2018527902 A5 JP 2018527902A5
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- 238000000034 method Methods 0.000 claims 49
- 239000000523 sample Substances 0.000 claims 29
- 102000004169 proteins and genes Human genes 0.000 claims 17
- 108090000623 proteins and genes Proteins 0.000 claims 17
- 108091034117 Oligonucleotide Proteins 0.000 claims 11
- 210000004027 cell Anatomy 0.000 claims 10
- 238000001514 detection method Methods 0.000 claims 7
- 210000004895 subcellular structure Anatomy 0.000 claims 7
- 238000002493 microarray Methods 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 238000006243 chemical reaction Methods 0.000 claims 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims 3
- 238000000746 purification Methods 0.000 claims 3
- 241000972773 Aulopiformes Species 0.000 claims 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
- 229920006318 anionic polymer Polymers 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- 229960000633 dextran sulfate Drugs 0.000 claims 2
- 238000009396 hybridization Methods 0.000 claims 2
- 230000001678 irradiating effect Effects 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 235000019515 salmon Nutrition 0.000 claims 2
- 108091023037 Aptamer Proteins 0.000 claims 1
- 102100034343 Integrase Human genes 0.000 claims 1
- 108010043958 Peptoids Proteins 0.000 claims 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
- 230000002159 abnormal effect Effects 0.000 claims 1
- 238000001261 affinity purification Methods 0.000 claims 1
- 210000004748 cultured cell Anatomy 0.000 claims 1
- 238000005286 illumination Methods 0.000 claims 1
- 238000004949 mass spectrometry Methods 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
- 239000012188 paraffin wax Substances 0.000 claims 1
- 238000002331 protein detection Methods 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 238000012800 visualization Methods 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021178589A JP2022020721A (ja) | 2015-07-17 | 2021-11-01 | 横断組織切片のユーザー定義領域における複数のタンパク質の同時定量 |
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562193819P | 2015-07-17 | 2015-07-17 | |
| US62/193,819 | 2015-07-17 | ||
| US201562261654P | 2015-12-01 | 2015-12-01 | |
| US62/261,654 | 2015-12-01 | ||
| US201662277283P | 2016-01-11 | 2016-01-11 | |
| US62/277,283 | 2016-01-11 | ||
| US201662323018P | 2016-04-15 | 2016-04-15 | |
| US62/323,018 | 2016-04-15 | ||
| PCT/US2016/042455 WO2017015097A1 (en) | 2015-07-17 | 2016-07-15 | Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021178589A Division JP2022020721A (ja) | 2015-07-17 | 2021-11-01 | 横断組織切片のユーザー定義領域における複数のタンパク質の同時定量 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018527902A JP2018527902A (ja) | 2018-09-27 |
| JP2018527902A5 true JP2018527902A5 (enExample) | 2019-09-12 |
| JP6971219B2 JP6971219B2 (ja) | 2021-11-24 |
Family
ID=56550406
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018502120A Active JP6971219B2 (ja) | 2015-07-17 | 2016-07-15 | 横断組織切片のユーザー定義領域における複数のタンパク質の同時定量 |
| JP2021178589A Pending JP2022020721A (ja) | 2015-07-17 | 2021-11-01 | 横断組織切片のユーザー定義領域における複数のタンパク質の同時定量 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021178589A Pending JP2022020721A (ja) | 2015-07-17 | 2021-11-01 | 横断組織切片のユーザー定義領域における複数のタンパク質の同時定量 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US10501777B2 (enExample) |
| EP (2) | EP4368992A3 (enExample) |
| JP (2) | JP6971219B2 (enExample) |
| KR (1) | KR102545430B1 (enExample) |
| CN (1) | CN108350483A (enExample) |
| AU (2) | AU2016297513B2 (enExample) |
| ES (1) | ES2975361T3 (enExample) |
| SG (1) | SG10202107055SA (enExample) |
| WO (1) | WO2017015097A1 (enExample) |
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| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| EP2971184B1 (en) | 2013-03-12 | 2019-04-17 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| LT3013984T (lt) | 2013-06-25 | 2023-06-12 | Prognosys Biosciences, Inc. | Biologinių taikinių erdvinių modelių mėginyje nustatymo būdai |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
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