EP4220162A3 - Connector, connector-label conjugate and marker for analysing biological samples - Google Patents

Connector, connector-label conjugate and marker for analysing biological samples Download PDF

Info

Publication number
EP4220162A3
EP4220162A3 EP22208330.5A EP22208330A EP4220162A3 EP 4220162 A3 EP4220162 A3 EP 4220162A3 EP 22208330 A EP22208330 A EP 22208330A EP 4220162 A3 EP4220162 A3 EP 4220162A3
Authority
EP
European Patent Office
Prior art keywords
connector
marker
biological samples
label conjugate
interactor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP22208330.5A
Other languages
German (de)
French (fr)
Other versions
EP4220162A2 (en
Inventor
Soeren Alsheimer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leica Microsystems CMS GmbH
Original Assignee
Leica Microsystems CMS GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP22154515.5A external-priority patent/EP4092414A1/en
Application filed by Leica Microsystems CMS GmbH filed Critical Leica Microsystems CMS GmbH
Publication of EP4220162A2 publication Critical patent/EP4220162A2/en
Publication of EP4220162A3 publication Critical patent/EP4220162A3/en
Priority to US18/510,752 priority Critical patent/US20240168031A1/en
Priority to CN202311546494.9A priority patent/CN118056910A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873—Cutting or cleaving
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A connector (802) for analysing biological sample is provided comprising: a nucleic acid backbone (804); at least one linker oligonucleotide (806) comprising a first affinity interactor (808), the linker oligonucleotide (806) at least partially hybridised to a complementary part of the nucleic acid backbone (804). The first affinity interactor (808) is configured to specifically bind to a label (810) comprising a second affinity interactor (820). In further aspects, a connector-label conjugate (1000), a marker (800, 1002) and method and device to generate those are provided.
EP22208330.5A 2022-02-01 2022-11-18 Connector, connector-label conjugate and marker for analysing biological samples Pending EP4220162A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US18/510,752 US20240168031A1 (en) 2022-02-01 2023-11-16 Connector, connector-label conjugate and marker for analysing biological samples
CN202311546494.9A CN118056910A (en) 2022-02-01 2023-11-20 Connector, connector-tag conjugate and label for analysis of biological samples

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP22154515.5A EP4092414A1 (en) 2021-05-19 2022-02-01 Connector, marker and method for analysing biological samples

Publications (2)

Publication Number Publication Date
EP4220162A2 EP4220162A2 (en) 2023-08-02
EP4220162A3 true EP4220162A3 (en) 2023-10-18

Family

ID=84331037

Family Applications (1)

Application Number Title Priority Date Filing Date
EP22208330.5A Pending EP4220162A3 (en) 2022-02-01 2022-11-18 Connector, connector-label conjugate and marker for analysing biological samples

Country Status (3)

Country Link
US (1) US20240168031A1 (en)
EP (1) EP4220162A3 (en)
CN (2) CN116539570A (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200040382A1 (en) * 2015-07-17 2020-02-06 Nanostring Technologies, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
US20200080130A1 (en) * 2018-05-30 2020-03-12 Generation Biotech, Llc Method for capturing long polynucleotides for sequencing specific gnomic regions.

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012107719B4 (en) 2012-08-22 2017-09-21 Technische Universität Braunschweig Standard based on DNA origami

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200040382A1 (en) * 2015-07-17 2020-02-06 Nanostring Technologies, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
US20200080130A1 (en) * 2018-05-30 2020-03-12 Generation Biotech, Llc Method for capturing long polynucleotides for sequencing specific gnomic regions.

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"Biotechnology", 1 January 2016, ELSEVIER, ISBN: 978-0-12-385015-7, article CLARK DAVID P. ET AL: "Recombinant DNA Technology", pages: 63 - 95, XP093048384, DOI: 10.1016/B978-0-12-385015-7.00003-X *
ENGELEN W. ET AL: "DNA-based control of protein activity", CHEMICAL COMMUNICATIONS, vol. 52, no. 18, 1 January 2016 (2016-01-01), UK, pages 3598 - 3610, XP093048400, ISSN: 1359-7345, Retrieved from the Internet <URL:https://pubs.rsc.org/en/content/articlepdf/2016/cc/c5cc09853j> DOI: 10.1039/C5CC09853J *
HELLMEIER JOSCHKA ET AL: "Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact Proteins", ACS NANO, vol. 15, no. 9, 31 August 2021 (2021-08-31), US, pages 15057 - 15068, XP093048391, ISSN: 1936-0851, Retrieved from the Internet <URL:https://pubs.acs.org/doi/pdf/10.1021/acsnano.1c05411> DOI: 10.1021/acsnano.1c05411 *
HELLMEIER JOSCHKA ET AL: "Supplement to Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact Proteins", ACS NANO, vol. 15, no. 9, 28 September 2021 (2021-09-28), US, pages 1 - 31, XP093048396, ISSN: 1936-0851, DOI: 10.1021/acsnano.1c05411 *
MAKINO KOKI ET AL: "Color-Changing Fluorescent Barcode Based on Strand Displacement Reaction Enables Simple Multiplexed Labeling", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 144, no. 4, 20 January 2022 (2022-01-20), pages 1572 - 1579, XP055941768, ISSN: 0002-7863, DOI: 10.1021/jacs.1c09844 *
WALKER ADRIENNE: "Novel DNA Origami Based Lateral Flow Assay Development", THESIS, 1 May 2019 (2019-05-01), XP055870732, Retrieved from the Internet <URL:https://mds.marshall.edu/cgi/viewcontent.cgi?article=2310&context=etd> [retrieved on 20211208] *

Also Published As

Publication number Publication date
CN118056910A (en) 2024-05-21
CN116539570A (en) 2023-08-04
EP4220162A2 (en) 2023-08-02
US20240168031A1 (en) 2024-05-23

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