KR102545430B1 - 절편화된 조직의 사용자-한정된 영역에서의 복수의 단백질의 동시적인 정량화 - Google Patents
절편화된 조직의 사용자-한정된 영역에서의 복수의 단백질의 동시적인 정량화 Download PDFInfo
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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- C12Q2523/00—Reactions characterised by treatment of reaction samples
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- C12Q2523/319—Photocleavage, photolysis, photoactivation
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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- C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
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- C12Q2543/00—Reactions characterised by the reaction site, e.g. cell or chromosome
- C12Q2543/10—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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- G—PHYSICS
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
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Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562193819P | 2015-07-17 | 2015-07-17 | |
| US62/193,819 | 2015-07-17 | ||
| US201562261654P | 2015-12-01 | 2015-12-01 | |
| US62/261,654 | 2015-12-01 | ||
| US201662277283P | 2016-01-11 | 2016-01-11 | |
| US62/277,283 | 2016-01-11 | ||
| US201662323018P | 2016-04-15 | 2016-04-15 | |
| US62/323,018 | 2016-04-15 | ||
| PCT/US2016/042455 WO2017015097A1 (en) | 2015-07-17 | 2016-07-15 | Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20180039643A KR20180039643A (ko) | 2018-04-18 |
| KR102545430B1 true KR102545430B1 (ko) | 2023-06-19 |
Family
ID=56550406
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020187004423A Active KR102545430B1 (ko) | 2015-07-17 | 2016-07-15 | 절편화된 조직의 사용자-한정된 영역에서의 복수의 단백질의 동시적인 정량화 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US10501777B2 (enExample) |
| EP (2) | EP3325649B1 (enExample) |
| JP (2) | JP6971219B2 (enExample) |
| KR (1) | KR102545430B1 (enExample) |
| CN (1) | CN108350483A (enExample) |
| AU (2) | AU2016297513B2 (enExample) |
| ES (1) | ES2975361T3 (enExample) |
| SG (1) | SG10202107055SA (enExample) |
| WO (1) | WO2017015097A1 (enExample) |
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| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| DK3901281T4 (da) | 2015-04-10 | 2025-10-20 | Illumina Inc | Rumligt adskilt, multipleks nukleinsyreanalyse af biologiske prøver |
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