JP2017537649A5 - - Google Patents
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- JP2017537649A5 JP2017537649A5 JP2017532641A JP2017532641A JP2017537649A5 JP 2017537649 A5 JP2017537649 A5 JP 2017537649A5 JP 2017532641 A JP2017532641 A JP 2017532641A JP 2017532641 A JP2017532641 A JP 2017532641A JP 2017537649 A5 JP2017537649 A5 JP 2017537649A5
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- Prior art keywords
- ptf1a
- endoderm cells
- ngn3
- cells
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000004039 endoderm cell Anatomy 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 21
- 210000004027 cell Anatomy 0.000 claims description 18
- 101000603702 Homo sapiens Neurogenin-3 Proteins 0.000 claims description 15
- 102100038553 Neurogenin-3 Human genes 0.000 claims description 15
- 230000014509 gene expression Effects 0.000 claims description 14
- 210000002438 upper gastrointestinal tract Anatomy 0.000 claims description 14
- 238000012258 culturing Methods 0.000 claims description 11
- 230000009996 pancreatic endocrine effect Effects 0.000 claims description 5
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims description 4
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims description 4
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims description 4
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 claims description 4
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 claims description 4
- 230000011664 signaling Effects 0.000 claims description 4
- 238000004114 suspension culture Methods 0.000 claims description 4
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims description 2
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims description 2
- 230000004913 activation Effects 0.000 claims description 2
- 230000000903 blocking effect Effects 0.000 claims description 2
- 230000004186 co-expression Effects 0.000 claims description 2
- 230000004069 differentiation Effects 0.000 claims description 2
- 210000003372 endocrine gland Anatomy 0.000 claims description 2
- 239000003112 inhibitor Substances 0.000 claims description 2
- 230000005764 inhibitory process Effects 0.000 claims description 2
- 150000004492 retinoid derivatives Chemical class 0.000 claims description 2
- 230000008410 smoothened signaling pathway Effects 0.000 claims description 2
- 230000000638 stimulation Effects 0.000 claims description 2
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- 108010020382 Hepatocyte Nuclear Factor 1-alpha Proteins 0.000 claims 2
- 102100022057 Hepatocyte nuclear factor 1-alpha Human genes 0.000 claims 2
- 101000738523 Homo sapiens Pancreas transcription factor 1 subunit alpha Proteins 0.000 description 5
- 102100037878 Pancreas transcription factor 1 subunit alpha Human genes 0.000 description 5
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 4
- 210000003890 endocrine cell Anatomy 0.000 description 3
- 101100518002 Danio rerio nkx2.2a gene Proteins 0.000 description 2
- 108700014808 Homeobox Protein Nkx-2.2 Proteins 0.000 description 2
- 102100027886 Homeobox protein Nkx-2.2 Human genes 0.000 description 2
- 101001053263 Homo sapiens Insulin gene enhancer protein ISL-1 Proteins 0.000 description 2
- 101100460496 Homo sapiens NKX2-2 gene Proteins 0.000 description 2
- 101000613495 Homo sapiens Paired box protein Pax-4 Proteins 0.000 description 2
- 102000004877 Insulin Human genes 0.000 description 2
- 108090001061 Insulin Proteins 0.000 description 2
- 102100024392 Insulin gene enhancer protein ISL-1 Human genes 0.000 description 2
- 102100032063 Neurogenic differentiation factor 1 Human genes 0.000 description 2
- 108010032788 PAX6 Transcription Factor Proteins 0.000 description 2
- 102100040909 Paired box protein Pax-4 Human genes 0.000 description 2
- 102100037506 Paired box protein Pax-6 Human genes 0.000 description 2
- 230000024245 cell differentiation Effects 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940125396 insulin Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- HFDKKNHCYWNNNQ-YOGANYHLSA-N 75976-10-2 Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@@H](NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](C)N)C(C)C)[C@@H](C)O)C1=CC=C(O)C=C1 HFDKKNHCYWNNNQ-YOGANYHLSA-N 0.000 description 1
- 101000923091 Danio rerio Aristaless-related homeobox protein Proteins 0.000 description 1
- 101800001586 Ghrelin Proteins 0.000 description 1
- 102400000442 Ghrelin-28 Human genes 0.000 description 1
- 102400000321 Glucagon Human genes 0.000 description 1
- 108060003199 Glucagon Proteins 0.000 description 1
- -1 HNF3β Proteins 0.000 description 1
- 102100031470 Homeobox protein ARX Human genes 0.000 description 1
- 101000923090 Homo sapiens Homeobox protein ARX Proteins 0.000 description 1
- 101000971533 Homo sapiens Killer cell lectin-like receptor subfamily G member 1 Proteins 0.000 description 1
- 101000979205 Homo sapiens Transcription factor MafA Proteins 0.000 description 1
- 102100021457 Killer cell lectin-like receptor subfamily G member 1 Human genes 0.000 description 1
- 101150079937 NEUROD1 gene Proteins 0.000 description 1
- 108700020297 NeuroD Proteins 0.000 description 1
- 108050000588 Neurogenic differentiation factor 1 Proteins 0.000 description 1
- 102000018886 Pancreatic Polypeptide Human genes 0.000 description 1
- 102000005157 Somatostatin Human genes 0.000 description 1
- 108010056088 Somatostatin Proteins 0.000 description 1
- 101000983124 Sus scrofa Pancreatic prohormone precursor Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 description 1
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- MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 description 1
- 229960004666 glucagon Drugs 0.000 description 1
- OGQSCIYDJSNCMY-UHFFFAOYSA-H iron(3+);methyl-dioxido-oxo-$l^{5}-arsane Chemical compound [Fe+3].[Fe+3].C[As]([O-])([O-])=O.C[As]([O-])([O-])=O.C[As]([O-])([O-])=O OGQSCIYDJSNCMY-UHFFFAOYSA-H 0.000 description 1
- NHXLMOGPVYXJNR-ATOGVRKGSA-N somatostatin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CSSC[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C3=CC=CC=C3NC=2)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N1)[C@@H](C)O)NC(=O)CNC(=O)[C@H](C)N)C(O)=O)=O)[C@H](O)C)C1=CC=CC=C1 NHXLMOGPVYXJNR-ATOGVRKGSA-N 0.000 description 1
- 229960000553 somatostatin Drugs 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462094509P | 2014-12-19 | 2014-12-19 | |
| US62/094,509 | 2014-12-19 | ||
| PCT/US2015/064713 WO2016100035A1 (en) | 2014-12-19 | 2015-12-09 | Suspension culturing of pluripotent stem cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017537649A JP2017537649A (ja) | 2017-12-21 |
| JP2017537649A5 true JP2017537649A5 (enExample) | 2019-02-07 |
| JP6800854B2 JP6800854B2 (ja) | 2020-12-16 |
Family
ID=56127372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017532641A Active JP6800854B2 (ja) | 2014-12-19 | 2015-12-09 | 多能性幹細胞の懸濁培養 |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20160215268A1 (enExample) |
| EP (1) | EP3234109A4 (enExample) |
| JP (1) | JP6800854B2 (enExample) |
| KR (3) | KR102519502B1 (enExample) |
| CN (1) | CN107250349A (enExample) |
| AR (1) | AR103080A1 (enExample) |
| AU (1) | AU2015363008B2 (enExample) |
| BR (1) | BR112017012974A2 (enExample) |
| CA (1) | CA2970935A1 (enExample) |
| MX (1) | MX2017008176A (enExample) |
| PH (1) | PH12017501153A1 (enExample) |
| RU (1) | RU2017125728A (enExample) |
| SG (1) | SG11201704961VA (enExample) |
| TW (1) | TW201636421A (enExample) |
| WO (1) | WO2016100035A1 (enExample) |
| ZA (1) | ZA201704868B (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3408375A4 (en) | 2016-01-26 | 2019-07-17 | Centre for Commercialization of Regenerative Medicine | PROCESS FOR DISSOCIATION OF CELL AGGREGATES |
| US10391156B2 (en) | 2017-07-12 | 2019-08-27 | Viacyte, Inc. | University donor cells and related methods |
| JP2020527956A (ja) * | 2017-07-21 | 2020-09-17 | センマ・セラピューティクス・インコーポレーテッド | 幹細胞由来の膵臓ベータ細胞の再凝集 |
| US20200199540A1 (en) * | 2017-09-11 | 2020-06-25 | Novo Nordisk A/S | Enrichment of nkx6.1 and c-peptide co-expressing cells derived in vitro from stem cells |
| CN118497104A (zh) | 2017-11-15 | 2024-08-16 | 沃泰克斯药物股份有限公司 | 胰岛细胞制备性组合物和使用方法 |
| EP3833365A4 (en) | 2018-08-10 | 2022-05-11 | Vertex Pharmaceuticals Incorporated | Stem cell derived islet differentiation |
| US10724052B2 (en) | 2018-09-07 | 2020-07-28 | Crispr Therapeutics Ag | Universal donor cells |
| TWI862513B (zh) * | 2018-09-19 | 2024-11-21 | 日商千紙鶴治療公司 | 包含胰島素產生細胞之細胞集團、包含該細胞集團之醫藥、其用途及製造方法 |
| CN113728090B (zh) * | 2019-04-08 | 2024-04-05 | 诺和诺德股份有限公司 | 从干细胞衍生的定形内胚层生成胰腺内胚层 |
| JP7555975B2 (ja) | 2019-05-31 | 2024-09-25 | ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド | 生体適合性メンブレン複合体 |
| WO2020243666A1 (en) | 2019-05-31 | 2020-12-03 | W. L. Gore & Associates, Inc. | A biocompatible membrane composite |
| JP2022534545A (ja) | 2019-05-31 | 2022-08-01 | ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド | 生体適合性メンブレン複合体 |
| CA3257300A1 (en) | 2019-05-31 | 2025-06-12 | W. L. Gore & Associates, Inc. | Cell encapsulation devices with controlled oxygen diffusion distances |
| US11118195B2 (en) | 2019-09-05 | 2021-09-14 | Crispr Therapeutics Ag | Universal donor cells |
| AU2020340622A1 (en) | 2019-09-05 | 2022-03-03 | Crispr Therapeutics Ag | Universal donor cells |
| KR20230146007A (ko) | 2020-12-31 | 2023-10-18 | 크리스퍼 테라퓨틱스 아게 | 범용 공여자 세포 |
| CN119432595A (zh) * | 2023-08-07 | 2025-02-14 | 浙江霍德生物工程有限公司 | 利用生物反应器进行诱导多能干细胞的悬浮培养 |
| WO2025117331A1 (en) * | 2023-12-01 | 2025-06-05 | Eli Lilly And Company | Methods of making stem cell-derived islet-like cells, as well as populations and compositions including the same |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| CN101240262A (zh) | 2001-12-07 | 2008-08-13 | 杰龙公司 | 源自人胚胎干细胞的胰岛细胞 |
| ES2564044T3 (es) | 2003-06-27 | 2016-03-17 | DePuy Synthes Products, Inc. | Células posparto derivadas de tejido del cordón umbilical y métodos de preparación y uso de las mismas |
| US20050266554A1 (en) * | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| JP4819697B2 (ja) | 2003-12-23 | 2011-11-24 | ヴィアサイト，インコーポレイテッド | 胚体内胚葉 |
| KR101178786B1 (ko) | 2004-03-23 | 2012-09-07 | 다이이찌 산쿄 가부시키가이샤 | 다능성 줄기세포의 증식 방법 |
| JP2008538276A (ja) | 2005-01-28 | 2008-10-23 | ノヴァセラ・リミテッド | 胚幹細胞培養のための方法 |
| AU2006202209B2 (en) | 2005-05-27 | 2011-04-14 | Lifescan, Inc. | Amniotic fluid derived cells |
| US8476070B2 (en) | 2005-08-29 | 2013-07-02 | Technion Research & Development Foundation Limited | Media for culturing stem cells |
| EP3584311A1 (en) * | 2005-10-27 | 2019-12-25 | Viacyte, Inc. | Pdx-1 expressing dorsal and ventral foregut endoderm |
| US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| DK2650359T3 (da) * | 2006-03-02 | 2022-06-13 | Viacyte Inc | Endokrine prekursorceller, pancreatiske hormon-udtrykkende celler og fremstillingsfremgangsmåder |
| AU2007277364B2 (en) | 2006-07-26 | 2010-08-12 | Viacyte, Inc. | Methods of producing pancreatic hormones |
| ES2704401T3 (es) | 2006-08-02 | 2019-03-18 | Technion Res & Dev Foundation | Métodos de expansión de células madre embrionarias en un cultivo en suspensión |
| DK2173863T3 (en) | 2007-06-29 | 2019-01-21 | Fujifilm Cellular Dynamics Inc | Automated method and apparatus for embryonic stem cell culture |
| KR101651661B1 (ko) * | 2008-06-30 | 2016-08-26 | 얀센 바이오테크 인코포레이티드 | 만능 줄기 세포의 분화 |
| WO2010022395A2 (en) * | 2008-08-22 | 2010-02-25 | President And Fellows Of Harvard College | Methods of reprogramming cells |
| CN102695790A (zh) | 2009-10-29 | 2012-09-26 | 詹森生物科技公司 | 多能干细胞 |
| WO2011139628A1 (en) * | 2010-04-25 | 2011-11-10 | Mount Sinai School Of Medicine | Generation of anterior foregut endoderm from pluripotent cells |
| US9388386B2 (en) * | 2011-12-22 | 2016-07-12 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
| RU2684215C2 (ru) * | 2012-12-31 | 2019-04-04 | Янссен Байотек, Инк. | Способ получения панкреатических эндокринных клеток (варианты) и способ увеличения выхода бета-клеток |
| US8859286B2 (en) | 2013-03-14 | 2014-10-14 | Viacyte, Inc. | In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells |
-
2015
- 2015-12-09 EP EP15870718.2A patent/EP3234109A4/en active Pending
- 2015-12-09 CN CN201580076646.0A patent/CN107250349A/zh active Pending
- 2015-12-09 MX MX2017008176A patent/MX2017008176A/es unknown
- 2015-12-09 AU AU2015363008A patent/AU2015363008B2/en active Active
- 2015-12-09 RU RU2017125728A patent/RU2017125728A/ru not_active Application Discontinuation
- 2015-12-09 CA CA2970935A patent/CA2970935A1/en active Pending
- 2015-12-09 US US14/963,730 patent/US20160215268A1/en not_active Abandoned
- 2015-12-09 BR BR112017012974A patent/BR112017012974A2/pt not_active Application Discontinuation
- 2015-12-09 KR KR1020217022958A patent/KR102519502B1/ko active Active
- 2015-12-09 KR KR1020177019548A patent/KR102281752B1/ko active Active
- 2015-12-09 JP JP2017532641A patent/JP6800854B2/ja active Active
- 2015-12-09 SG SG11201704961VA patent/SG11201704961VA/en unknown
- 2015-12-09 WO PCT/US2015/064713 patent/WO2016100035A1/en not_active Ceased
- 2015-12-09 KR KR1020237011455A patent/KR102700499B1/ko active Active
- 2015-12-17 TW TW104142402A patent/TW201636421A/zh unknown
- 2015-12-17 AR ARP150104154A patent/AR103080A1/es unknown
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2017
- 2017-06-19 PH PH12017501153A patent/PH12017501153A1/en unknown
- 2017-07-18 ZA ZA2017/04868A patent/ZA201704868B/en unknown
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