RU2017125728A - Культивирование плюрипотентных стволовых клеток в суспензии - Google Patents
Культивирование плюрипотентных стволовых клеток в суспензии Download PDFInfo
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- RU2017125728A RU2017125728A RU2017125728A RU2017125728A RU2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A
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- Prior art keywords
- cells
- expression
- endoderm
- ptf1a
- differentiating
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- 210000001778 pluripotent stem cell Anatomy 0.000 title 1
- 239000000725 suspension Substances 0.000 title 1
- 210000004027 cell Anatomy 0.000 claims 13
- 210000004039 endoderm cell Anatomy 0.000 claims 9
- 238000000034 method Methods 0.000 claims 7
- 101000738523 Homo sapiens Pancreas transcription factor 1 subunit alpha Proteins 0.000 claims 5
- 102100037878 Pancreas transcription factor 1 subunit alpha Human genes 0.000 claims 5
- 230000014509 gene expression Effects 0.000 claims 5
- 230000000968 intestinal effect Effects 0.000 claims 5
- 238000012258 culturing Methods 0.000 claims 4
- 101000603702 Homo sapiens Neurogenin-3 Proteins 0.000 claims 3
- 102100038553 Neurogenin-3 Human genes 0.000 claims 3
- 210000001900 endoderm Anatomy 0.000 claims 3
- 230000019491 signal transduction Effects 0.000 claims 3
- 238000004114 suspension culture Methods 0.000 claims 2
- 102000003693 Hedgehog Proteins Human genes 0.000 claims 1
- 108090000031 Hedgehog Proteins Proteins 0.000 claims 1
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims 1
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims 1
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims 1
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 claims 1
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 claims 1
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 1
- 230000004913 activation Effects 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 230000004186 co-expression Effects 0.000 claims 1
- 230000004069 differentiation Effects 0.000 claims 1
- 210000003890 endocrine cell Anatomy 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 108091006086 inhibitor proteins Proteins 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 210000000936 intestine Anatomy 0.000 claims 1
- 230000009996 pancreatic endocrine effect Effects 0.000 claims 1
- 150000004492 retinoid derivatives Chemical class 0.000 claims 1
- 230000004936 stimulating effect Effects 0.000 claims 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 claims 1
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
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- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- Bioinformatics & Cheminformatics (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462094509P | 2014-12-19 | 2014-12-19 | |
| US62/094,509 | 2014-12-19 | ||
| PCT/US2015/064713 WO2016100035A1 (en) | 2014-12-19 | 2015-12-09 | Suspension culturing of pluripotent stem cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2017125728A true RU2017125728A (ru) | 2019-01-22 |
| RU2017125728A3 RU2017125728A3 (enExample) | 2019-06-03 |
Family
ID=56127372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2017125728A RU2017125728A (ru) | 2014-12-19 | 2015-12-09 | Культивирование плюрипотентных стволовых клеток в суспензии |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20160215268A1 (enExample) |
| EP (1) | EP3234109A4 (enExample) |
| JP (1) | JP6800854B2 (enExample) |
| KR (3) | KR102519502B1 (enExample) |
| CN (1) | CN107250349A (enExample) |
| AR (1) | AR103080A1 (enExample) |
| AU (1) | AU2015363008B2 (enExample) |
| BR (1) | BR112017012974A2 (enExample) |
| CA (1) | CA2970935A1 (enExample) |
| MX (1) | MX2017008176A (enExample) |
| PH (1) | PH12017501153A1 (enExample) |
| RU (1) | RU2017125728A (enExample) |
| SG (1) | SG11201704961VA (enExample) |
| TW (1) | TW201636421A (enExample) |
| WO (1) | WO2016100035A1 (enExample) |
| ZA (1) | ZA201704868B (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3408375A4 (en) | 2016-01-26 | 2019-07-17 | Centre for Commercialization of Regenerative Medicine | PROCESS FOR DISSOCIATION OF CELL AGGREGATES |
| US10391156B2 (en) | 2017-07-12 | 2019-08-27 | Viacyte, Inc. | University donor cells and related methods |
| JP2020527956A (ja) * | 2017-07-21 | 2020-09-17 | センマ・セラピューティクス・インコーポレーテッド | 幹細胞由来の膵臓ベータ細胞の再凝集 |
| US20200199540A1 (en) * | 2017-09-11 | 2020-06-25 | Novo Nordisk A/S | Enrichment of nkx6.1 and c-peptide co-expressing cells derived in vitro from stem cells |
| CN118497104A (zh) | 2017-11-15 | 2024-08-16 | 沃泰克斯药物股份有限公司 | 胰岛细胞制备性组合物和使用方法 |
| EP3833365A4 (en) | 2018-08-10 | 2022-05-11 | Vertex Pharmaceuticals Incorporated | Stem cell derived islet differentiation |
| US10724052B2 (en) | 2018-09-07 | 2020-07-28 | Crispr Therapeutics Ag | Universal donor cells |
| TWI862513B (zh) * | 2018-09-19 | 2024-11-21 | 日商千紙鶴治療公司 | 包含胰島素產生細胞之細胞集團、包含該細胞集團之醫藥、其用途及製造方法 |
| CN113728090B (zh) * | 2019-04-08 | 2024-04-05 | 诺和诺德股份有限公司 | 从干细胞衍生的定形内胚层生成胰腺内胚层 |
| JP7555975B2 (ja) | 2019-05-31 | 2024-09-25 | ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド | 生体適合性メンブレン複合体 |
| WO2020243666A1 (en) | 2019-05-31 | 2020-12-03 | W. L. Gore & Associates, Inc. | A biocompatible membrane composite |
| JP2022534545A (ja) | 2019-05-31 | 2022-08-01 | ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド | 生体適合性メンブレン複合体 |
| CA3257300A1 (en) | 2019-05-31 | 2025-06-12 | W. L. Gore & Associates, Inc. | Cell encapsulation devices with controlled oxygen diffusion distances |
| US11118195B2 (en) | 2019-09-05 | 2021-09-14 | Crispr Therapeutics Ag | Universal donor cells |
| AU2020340622A1 (en) | 2019-09-05 | 2022-03-03 | Crispr Therapeutics Ag | Universal donor cells |
| KR20230146007A (ko) | 2020-12-31 | 2023-10-18 | 크리스퍼 테라퓨틱스 아게 | 범용 공여자 세포 |
| CN119432595A (zh) * | 2023-08-07 | 2025-02-14 | 浙江霍德生物工程有限公司 | 利用生物反应器进行诱导多能干细胞的悬浮培养 |
| WO2025117331A1 (en) * | 2023-12-01 | 2025-06-05 | Eli Lilly And Company | Methods of making stem cell-derived islet-like cells, as well as populations and compositions including the same |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| CN101240262A (zh) | 2001-12-07 | 2008-08-13 | 杰龙公司 | 源自人胚胎干细胞的胰岛细胞 |
| ES2564044T3 (es) | 2003-06-27 | 2016-03-17 | DePuy Synthes Products, Inc. | Células posparto derivadas de tejido del cordón umbilical y métodos de preparación y uso de las mismas |
| US20050266554A1 (en) * | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| JP4819697B2 (ja) | 2003-12-23 | 2011-11-24 | ヴィアサイト，インコーポレイテッド | 胚体内胚葉 |
| KR101178786B1 (ko) | 2004-03-23 | 2012-09-07 | 다이이찌 산쿄 가부시키가이샤 | 다능성 줄기세포의 증식 방법 |
| JP2008538276A (ja) | 2005-01-28 | 2008-10-23 | ノヴァセラ・リミテッド | 胚幹細胞培養のための方法 |
| AU2006202209B2 (en) | 2005-05-27 | 2011-04-14 | Lifescan, Inc. | Amniotic fluid derived cells |
| US8476070B2 (en) | 2005-08-29 | 2013-07-02 | Technion Research & Development Foundation Limited | Media for culturing stem cells |
| EP3584311A1 (en) * | 2005-10-27 | 2019-12-25 | Viacyte, Inc. | Pdx-1 expressing dorsal and ventral foregut endoderm |
| US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| DK2650359T3 (da) * | 2006-03-02 | 2022-06-13 | Viacyte Inc | Endokrine prekursorceller, pancreatiske hormon-udtrykkende celler og fremstillingsfremgangsmåder |
| AU2007277364B2 (en) | 2006-07-26 | 2010-08-12 | Viacyte, Inc. | Methods of producing pancreatic hormones |
| ES2704401T3 (es) | 2006-08-02 | 2019-03-18 | Technion Res & Dev Foundation | Métodos de expansión de células madre embrionarias en un cultivo en suspensión |
| DK2173863T3 (en) | 2007-06-29 | 2019-01-21 | Fujifilm Cellular Dynamics Inc | Automated method and apparatus for embryonic stem cell culture |
| KR101651661B1 (ko) * | 2008-06-30 | 2016-08-26 | 얀센 바이오테크 인코포레이티드 | 만능 줄기 세포의 분화 |
| WO2010022395A2 (en) * | 2008-08-22 | 2010-02-25 | President And Fellows Of Harvard College | Methods of reprogramming cells |
| CN102695790A (zh) | 2009-10-29 | 2012-09-26 | 詹森生物科技公司 | 多能干细胞 |
| WO2011139628A1 (en) * | 2010-04-25 | 2011-11-10 | Mount Sinai School Of Medicine | Generation of anterior foregut endoderm from pluripotent cells |
| US9388386B2 (en) * | 2011-12-22 | 2016-07-12 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
| RU2684215C2 (ru) * | 2012-12-31 | 2019-04-04 | Янссен Байотек, Инк. | Способ получения панкреатических эндокринных клеток (варианты) и способ увеличения выхода бета-клеток |
| US8859286B2 (en) | 2013-03-14 | 2014-10-14 | Viacyte, Inc. | In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells |
-
2015
- 2015-12-09 EP EP15870718.2A patent/EP3234109A4/en active Pending
- 2015-12-09 CN CN201580076646.0A patent/CN107250349A/zh active Pending
- 2015-12-09 MX MX2017008176A patent/MX2017008176A/es unknown
- 2015-12-09 AU AU2015363008A patent/AU2015363008B2/en active Active
- 2015-12-09 RU RU2017125728A patent/RU2017125728A/ru not_active Application Discontinuation
- 2015-12-09 CA CA2970935A patent/CA2970935A1/en active Pending
- 2015-12-09 US US14/963,730 patent/US20160215268A1/en not_active Abandoned
- 2015-12-09 BR BR112017012974A patent/BR112017012974A2/pt not_active Application Discontinuation
- 2015-12-09 KR KR1020217022958A patent/KR102519502B1/ko active Active
- 2015-12-09 KR KR1020177019548A patent/KR102281752B1/ko active Active
- 2015-12-09 JP JP2017532641A patent/JP6800854B2/ja active Active
- 2015-12-09 SG SG11201704961VA patent/SG11201704961VA/en unknown
- 2015-12-09 WO PCT/US2015/064713 patent/WO2016100035A1/en not_active Ceased
- 2015-12-09 KR KR1020237011455A patent/KR102700499B1/ko active Active
- 2015-12-17 TW TW104142402A patent/TW201636421A/zh unknown
- 2015-12-17 AR ARP150104154A patent/AR103080A1/es unknown
-
2017
- 2017-06-19 PH PH12017501153A patent/PH12017501153A1/en unknown
- 2017-07-18 ZA ZA2017/04868A patent/ZA201704868B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| KR102700499B1 (ko) | 2024-08-28 |
| US20160215268A1 (en) | 2016-07-28 |
| EP3234109A4 (en) | 2018-06-27 |
| AU2015363008A1 (en) | 2017-06-29 |
| CA2970935A1 (en) | 2016-06-23 |
| KR102281752B1 (ko) | 2021-07-23 |
| WO2016100035A1 (en) | 2016-06-23 |
| BR112017012974A2 (pt) | 2018-01-09 |
| EP3234109A1 (en) | 2017-10-25 |
| JP2017537649A (ja) | 2017-12-21 |
| SG11201704961VA (en) | 2017-07-28 |
| AR103080A1 (es) | 2017-04-12 |
| TW201636421A (zh) | 2016-10-16 |
| KR20230050478A (ko) | 2023-04-14 |
| MX2017008176A (es) | 2018-02-09 |
| PH12017501153A1 (en) | 2017-11-27 |
| KR20210094141A (ko) | 2021-07-28 |
| KR102519502B1 (ko) | 2023-04-06 |
| KR20170087520A (ko) | 2017-07-28 |
| AU2015363008B2 (en) | 2021-12-09 |
| ZA201704868B (en) | 2019-02-27 |
| CN107250349A (zh) | 2017-10-13 |
| RU2017125728A3 (enExample) | 2019-06-03 |
| JP6800854B2 (ja) | 2020-12-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20191021 |