AU2015363008B2 - Suspension culturing of pluripotent stem cells - Google Patents

Suspension culturing of pluripotent stem cells Download PDF

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AU2015363008B2
AU2015363008B2 AU2015363008A AU2015363008A AU2015363008B2 AU 2015363008 B2 AU2015363008 B2 AU 2015363008B2 AU 2015363008 A AU2015363008 A AU 2015363008A AU 2015363008 A AU2015363008 A AU 2015363008A AU 2015363008 B2 AU2015363008 B2 AU 2015363008B2
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cells
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day
expression
cell
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AU2015363008A1 (en
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Benjamin FRYERS
Daina LANIAUSKAS
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Janssen Biotech Inc
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/395—Thyroid hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2015363008A 2014-12-19 2015-12-09 Suspension culturing of pluripotent stem cells Active AU2015363008B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462094509P 2014-12-19 2014-12-19
US62/094,509 2014-12-19
PCT/US2015/064713 WO2016100035A1 (en) 2014-12-19 2015-12-09 Suspension culturing of pluripotent stem cells

Publications (2)

Publication Number Publication Date
AU2015363008A1 AU2015363008A1 (en) 2017-06-29
AU2015363008B2 true AU2015363008B2 (en) 2021-12-09

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AU2015363008A Active AU2015363008B2 (en) 2014-12-19 2015-12-09 Suspension culturing of pluripotent stem cells

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US (1) US20160215268A1 (enExample)
EP (1) EP3234109A4 (enExample)
JP (1) JP6800854B2 (enExample)
KR (3) KR102281752B1 (enExample)
CN (1) CN107250349A (enExample)
AR (1) AR103080A1 (enExample)
AU (1) AU2015363008B2 (enExample)
BR (1) BR112017012974A2 (enExample)
CA (1) CA2970935A1 (enExample)
MX (1) MX2017008176A (enExample)
PH (1) PH12017501153A1 (enExample)
RU (1) RU2017125728A (enExample)
SG (1) SG11201704961VA (enExample)
TW (1) TW201636421A (enExample)
WO (1) WO2016100035A1 (enExample)
ZA (1) ZA201704868B (enExample)

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US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
EP4488363A3 (en) * 2017-07-21 2025-03-26 Vertex Pharmaceuticals Incorporated Re-aggregation of stem cell-derived pancreatic beta cells
AU2018330499B2 (en) * 2017-09-11 2025-01-30 Novo Nordisk A/S Enrichment of NKX6.1 and C-peptide co-expressing cells derived in vitro from stem cells
AU2018370029B2 (en) 2017-11-15 2024-11-07 Vertex Pharmaceuticals Incorporated Islet cell manufacturing compositions and methods of use
AU2019320072A1 (en) 2018-08-10 2021-02-25 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
US12378572B2 (en) 2018-09-07 2025-08-05 Crispr Therapeutics Ag Universal donor cells
SG11202102748WA (en) * 2018-09-19 2021-04-29 Takeda Pharmaceuticals Co Insulin-producing cells
EP3953451A1 (en) * 2019-04-08 2022-02-16 Novo Nordisk A/S Generation of pancreatic endoderm from stem cell derived definitive endoderm
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
CA3139585C (en) 2019-05-31 2024-01-23 W. L. Gore & Associates, Inc. A biocompatible membrane composite
CA3139292C (en) 2019-05-31 2025-06-10 Viacyte, Inc. CELL ENCAPSULATION DEVICES WITH REGULATED OXYGEN DIFFUSION DISTANCES
US20220234006A1 (en) 2019-05-31 2022-07-28 W. L. Gore & Associates, Inc. A biocompatible membrane composite
CA3150233A1 (en) 2019-09-05 2021-03-11 Alireza Rezania Universal donor cells
CN114375300A (zh) 2019-09-05 2022-04-19 克里斯珀医疗股份公司 通用供体细胞
AU2021414405A1 (en) 2020-12-31 2023-08-10 Crispr Therapeutics Ag Universal donor cells
CN119432595A (zh) * 2023-08-07 2025-02-14 浙江霍德生物工程有限公司 利用生物反应器进行诱导多能干细胞的悬浮培养
WO2025117331A1 (en) * 2023-12-01 2025-06-05 Eli Lilly And Company Methods of making stem cell-derived islet-like cells, as well as populations and compositions including the same

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Also Published As

Publication number Publication date
TW201636421A (zh) 2016-10-16
CN107250349A (zh) 2017-10-13
JP2017537649A (ja) 2017-12-21
AU2015363008A1 (en) 2017-06-29
KR102700499B1 (ko) 2024-08-28
BR112017012974A2 (pt) 2018-01-09
WO2016100035A1 (en) 2016-06-23
US20160215268A1 (en) 2016-07-28
KR102281752B1 (ko) 2021-07-23
CA2970935A1 (en) 2016-06-23
RU2017125728A (ru) 2019-01-22
PH12017501153A1 (en) 2017-11-27
EP3234109A1 (en) 2017-10-25
MX2017008176A (es) 2018-02-09
SG11201704961VA (en) 2017-07-28
RU2017125728A3 (enExample) 2019-06-03
AR103080A1 (es) 2017-04-12
KR20170087520A (ko) 2017-07-28
KR20230050478A (ko) 2023-04-14
ZA201704868B (en) 2019-02-27
JP6800854B2 (ja) 2020-12-16
KR20210094141A (ko) 2021-07-28
KR102519502B1 (ko) 2023-04-06
EP3234109A4 (en) 2018-06-27

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