JP2017536114A5 - - Google Patents
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- Publication number
- JP2017536114A5 JP2017536114A5 JP2017524014A JP2017524014A JP2017536114A5 JP 2017536114 A5 JP2017536114 A5 JP 2017536114A5 JP 2017524014 A JP2017524014 A JP 2017524014A JP 2017524014 A JP2017524014 A JP 2017524014A JP 2017536114 A5 JP2017536114 A5 JP 2017536114A5
- Authority
- JP
- Japan
- Prior art keywords
- vnar
- amino acid
- encode
- domain
- codons
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000001413 amino acids Chemical class 0.000 claims 16
- 235000001014 amino acid Nutrition 0.000 claims 15
- 238000000034 method Methods 0.000 claims 11
- 108091033319 polynucleotide Proteins 0.000 claims 10
- 239000002157 polynucleotide Substances 0.000 claims 10
- 102000040430 polynucleotide Human genes 0.000 claims 10
- 108020004705 Codon Proteins 0.000 claims 7
- 235000018417 cysteine Nutrition 0.000 claims 6
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 6
- 239000000427 antigen Substances 0.000 claims 4
- 102000036639 antigens Human genes 0.000 claims 4
- 108091007433 antigens Proteins 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 239000004472 Lysine Substances 0.000 claims 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 3
- 239000004473 Threonine Substances 0.000 claims 3
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims 3
- 210000004899 c-terminal region Anatomy 0.000 claims 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 3
- 235000018102 proteins Nutrition 0.000 claims 3
- 108091006020 Fc-tagged proteins Proteins 0.000 claims 2
- 101710160107 Outer membrane protein A Proteins 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 239000013068 control sample Substances 0.000 claims 2
- 108020001507 fusion proteins Proteins 0.000 claims 2
- 102000037865 fusion proteins Human genes 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 108010032595 Antibody Binding Sites Proteins 0.000 claims 1
- 241000251730 Chondrichthyes Species 0.000 claims 1
- 101100421450 Drosophila melanogaster Shark gene Proteins 0.000 claims 1
- 238000002965 ELISA Methods 0.000 claims 1
- 108091007491 NSP3 Papain-like protease domains Proteins 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 102000007562 Serum Albumin Human genes 0.000 claims 1
- 108010071390 Serum Albumin Proteins 0.000 claims 1
- 201000011510 cancer Diseases 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000000684 flow cytometry Methods 0.000 claims 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 238000002823 phage display Methods 0.000 claims 1
- 238000003498 protein array Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14003700.3 | 2014-11-03 | ||
| EP14003700 | 2014-11-03 | ||
| PCT/EP2015/002086 WO2016070959A1 (en) | 2014-11-03 | 2015-10-21 | Methods for generating bispecific shark variable antibody domains and use thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017536114A JP2017536114A (ja) | 2017-12-07 |
| JP2017536114A5 true JP2017536114A5 (enExample) | 2018-11-29 |
| JP6734271B2 JP6734271B2 (ja) | 2020-08-05 |
Family
ID=51945671
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017524014A Expired - Fee Related JP6734271B2 (ja) | 2014-11-03 | 2015-10-21 | 二重特異性サメ抗体可変ドメインの製造方法およびそれらの使用 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US11001640B2 (enExample) |
| EP (1) | EP3215531A1 (enExample) |
| JP (1) | JP6734271B2 (enExample) |
| CN (1) | CN107074961A (enExample) |
| AU (1) | AU2015342264B2 (enExample) |
| CA (1) | CA2966397A1 (enExample) |
| IL (1) | IL252008A0 (enExample) |
| WO (1) | WO2016070959A1 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3033082A1 (en) * | 2016-08-06 | 2018-02-15 | Ossianix, Inc. | In vivo methods for selecting peptides that cross the blood brain barrier, related compositions and methods of use |
| CA3053010A1 (en) | 2017-02-08 | 2018-08-16 | Dragonfly Therapeutics, Inc. | Multi-specific binding proteins for activation of natural killer cells and therapeutic uses thereof to treat cancer |
| JP7685821B2 (ja) | 2017-02-20 | 2025-05-30 | ドラゴンフライ セラピューティクス， インコーポレイテッド | Ｈｅｒ２、ＮＫＧ２ＤおよびＣＤ１６に結合するタンパク質 |
| SG10202102251YA (en) * | 2017-08-16 | 2021-04-29 | Dragonfly Therapeutics Inc | Proteins binding nkg2d, cd16, and egfr, hla-e ccr4, or pd-l1 |
| CA3090236A1 (en) | 2018-02-08 | 2019-08-15 | Dragonfly Therapeutics, Inc. | Combination therapy of cancer involving multi-specific binding proteins that activate natural killer cells |
| US11884733B2 (en) | 2018-02-08 | 2024-01-30 | Dragonfly Therapeutics, Inc. | Antibody variable domains targeting the NKG2D receptor |
| KR102832460B1 (ko) | 2018-02-20 | 2025-07-11 | 드래곤플라이 쎄라퓨틱스, 인크. | Cd33, nkg2d, 및 cd16에 결합하는 다중-특이적 결합 단백질, 및 이의 사용 방법 |
| MA53284A (fr) | 2018-08-08 | 2022-01-26 | Dragonfly Therapeutics Inc | Protéines de liaison à nkg2d, à cd16 et à un antigène associé à une tumeur |
| AU2019318083A1 (en) | 2018-08-08 | 2021-02-25 | Dragonfly Therapeutics, Inc. | Multi-specific binding proteins that bind BCMA, NKG2D and CD16, and methods of use |
| EA202091888A1 (ru) | 2018-08-08 | 2020-10-23 | Драгонфлай Терапьютикс, Инк. | Вариабельные домены антител, нацеленные на рецептор nkg2d |
| WO2020056327A1 (en) | 2018-09-14 | 2020-03-19 | Ossianix, Inc. | Tfr-specific binding moieties and transcytosis method to select vnars that cross cellular barriers |
| IL297922A (en) | 2020-05-06 | 2023-01-01 | Dragonfly Therapeutics Inc | Proteins binding nkg2d, cd16 and clec12a |
| CN112301431B (zh) * | 2020-08-12 | 2023-01-31 | 华东理工大学 | 基于鲨鱼抗体可变区v-nar的噬菌体文库及其构建方法 |
| CN117222663A (zh) | 2021-03-03 | 2023-12-12 | 蜻蜓疗法股份有限公司 | 使用结合nkg2d、cd16和肿瘤相关抗原的多特异性结合蛋白治疗癌症的方法 |
| CN112940120B (zh) * | 2021-05-14 | 2021-08-17 | 深圳海创生物技术有限公司 | 降钙素原单域抗体及其应用和试剂盒 |
| CN118475603A (zh) * | 2021-11-03 | 2024-08-09 | 香港城市大学深圳研究院 | 高亲和力抗EGFP和抗SARS-CoV-2 vNAR单域抗体及其用途 |
| CN115975010A (zh) * | 2022-08-31 | 2023-04-18 | 闽江学院 | 一种十足目虹彩病毒1的单域抗体及其制备方法和应用 |
| CN116143913A (zh) * | 2022-12-30 | 2023-05-23 | 闽江学院 | 一种结合SARS-CoV-2 RBD的鲨鱼源单域抗体及其制备方法和应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004535182A (ja) | 2001-05-24 | 2004-11-25 | ザイモジェネティクス，インコーポレイティド | Ｔａｃｉ−免疫グロブリン融合タンパク質 |
| CA2788275A1 (en) * | 2010-03-03 | 2011-09-09 | Boehringer Ingelheim International Gmbh | Biparatopic abeta binding polypeptides |
| DK2606064T3 (en) * | 2010-08-16 | 2015-04-20 | Novimmune Sa | Methods for generating multispecific and multivalent antibodies |
| EP4151658A1 (en) | 2012-05-07 | 2023-03-22 | Elasmogen Limited | Single domain binding molecule |
| US10472410B2 (en) * | 2013-04-23 | 2019-11-12 | The University Court Of The University Of Aberdeen | Isolation of therapeutic target specific VNAR domains to ICOSL |
-
2015
- 2015-10-21 CN CN201580059697.2A patent/CN107074961A/zh active Pending
- 2015-10-21 US US15/523,888 patent/US11001640B2/en not_active Expired - Fee Related
- 2015-10-21 CA CA2966397A patent/CA2966397A1/en not_active Abandoned
- 2015-10-21 AU AU2015342264A patent/AU2015342264B2/en not_active Ceased
- 2015-10-21 JP JP2017524014A patent/JP6734271B2/ja not_active Expired - Fee Related
- 2015-10-21 WO PCT/EP2015/002086 patent/WO2016070959A1/en not_active Ceased
- 2015-10-21 EP EP15785053.8A patent/EP3215531A1/en not_active Withdrawn
-
2017
- 2017-04-27 IL IL252008A patent/IL252008A0/en unknown