JP2015519344A5 - - Google Patents
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- Publication number
- JP2015519344A5 JP2015519344A5 JP2015514137A JP2015514137A JP2015519344A5 JP 2015519344 A5 JP2015519344 A5 JP 2015519344A5 JP 2015514137 A JP2015514137 A JP 2015514137A JP 2015514137 A JP2015514137 A JP 2015514137A JP 2015519344 A5 JP2015519344 A5 JP 2015519344A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- reagent
- pore
- peptide
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003153 chemical reaction reagent Substances 0.000 claims 27
- 235000018102 proteins Nutrition 0.000 claims 26
- 102000004169 proteins and genes Human genes 0.000 claims 26
- 108090000623 proteins and genes Proteins 0.000 claims 26
- 210000004027 cell Anatomy 0.000 claims 20
- 238000000034 method Methods 0.000 claims 16
- 108090000765 processed proteins & peptides Proteins 0.000 claims 16
- 239000011148 porous material Substances 0.000 claims 15
- 230000001086 cytosolic effect Effects 0.000 claims 8
- 230000004927 fusion Effects 0.000 claims 7
- 239000000758 substrate Substances 0.000 claims 7
- 210000004899 c-terminal region Anatomy 0.000 claims 6
- 230000003278 mimic effect Effects 0.000 claims 6
- 150000001875 compounds Chemical class 0.000 claims 5
- 102000035118 modified proteins Human genes 0.000 claims 5
- 108091005573 modified proteins Proteins 0.000 claims 5
- 108020001580 protein domains Proteins 0.000 claims 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
- 230000027455 binding Effects 0.000 claims 4
- 235000018417 cysteine Nutrition 0.000 claims 4
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 4
- 230000004001 molecular interaction Effects 0.000 claims 4
- 150000007970 thio esters Chemical class 0.000 claims 4
- 235000001014 amino acid Nutrition 0.000 claims 3
- 150000002366 halogen compounds Chemical class 0.000 claims 3
- 125000004573 morpholin-4-yl group Chemical group N1(CCOCC1)* 0.000 claims 3
- 150000007523 nucleic acids Chemical group 0.000 claims 3
- 230000001225 therapeutic effect Effects 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 230000005526 G1 to G0 transition Effects 0.000 claims 2
- 108090000279 Peptidyltransferases Proteins 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 2
- 229960002685 biotin Drugs 0.000 claims 2
- 235000020958 biotin Nutrition 0.000 claims 2
- 239000011616 biotin Substances 0.000 claims 2
- 150000005829 chemical entities Chemical class 0.000 claims 2
- 108020001507 fusion proteins Proteins 0.000 claims 2
- 102000037865 fusion proteins Human genes 0.000 claims 2
- 230000003993 interaction Effects 0.000 claims 2
- 230000009870 specific binding Effects 0.000 claims 2
- 108010001857 Cell Surface Receptors Proteins 0.000 claims 1
- 101150029707 ERBB2 gene Proteins 0.000 claims 1
- -1 amino acid chemical entity Chemical class 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 230000002452 interceptive effect Effects 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 102000006240 membrane receptors Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 230000012743 protein tagging Effects 0.000 claims 1
- 230000008685 targeting Effects 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261649421P | 2012-05-21 | 2012-05-21 | |
| US201261649866P | 2012-05-21 | 2012-05-21 | |
| US61/649,421 | 2012-05-21 | ||
| US61/649,866 | 2012-05-21 | ||
| PCT/US2013/042118 WO2013177231A1 (en) | 2012-05-21 | 2013-05-21 | Translocation of non-natural chemical entities through anthrax protective antigen pore |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015519344A JP2015519344A (ja) | 2015-07-09 |
| JP2015519344A5 true JP2015519344A5 (enExample) | 2016-07-07 |
Family
ID=49624302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015514137A Pending JP2015519344A (ja) | 2012-05-21 | 2013-05-21 | 炭疽菌防御抗原ポアを通しての非天然化学実体のトランスロケーション |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US9731029B2 (enExample) |
| EP (1) | EP2852619A4 (enExample) |
| JP (1) | JP2015519344A (enExample) |
| CA (1) | CA2874436A1 (enExample) |
| WO (2) | WO2013177231A1 (enExample) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8673267B2 (en) | 2009-03-02 | 2014-03-18 | Massachusetts Institute Of Technology | Methods and products for in vivo enzyme profiling |
| US10006916B2 (en) | 2011-03-15 | 2018-06-26 | Massachusetts Institute Of Technology | Multiplexed detection with isotope-coded reporters |
| EP2920319B1 (en) | 2012-11-16 | 2020-02-19 | Poseida Therapeutics, Inc. | Site-specific enzymes and methods of use |
| EP2777714A1 (en) | 2013-03-15 | 2014-09-17 | NBE-Therapeutics LLC | Method of producing an immunoligand/payload conjugate by means of a sequence-specific transpeptidase enzyme |
| EP3004374B1 (en) | 2013-06-07 | 2020-08-12 | Massachusetts Institute of Technology | Affinity-based detection of ligand-encoded synthetic biomarkers |
| JP2017518082A (ja) * | 2014-06-17 | 2017-07-06 | ポセイダ セラピューティクス， インコーポレイテッド | ゲノム中の特異的遺伝子座にタンパク質を指向させるための方法およびその使用 |
| EP3234177A1 (en) | 2014-12-17 | 2017-10-25 | F. Hoffmann-La Roche AG | Activity assay for bond forming enzymes |
| JP6605606B2 (ja) | 2014-12-17 | 2019-11-13 | エフ．ホフマン−ラ ロシュ アーゲー | ソルターゼを使用した単一工程での二重ポリペプチドコンジュゲーションのための酵素ワンポット反応 |
| EP3929286A1 (en) | 2015-06-17 | 2021-12-29 | Poseida Therapeutics, Inc. | Compositions and methods for directing proteins to specific loci in the genome |
| EA201890587A1 (ru) | 2015-08-27 | 2018-09-28 | Президент Энд Феллоуз Оф Гарвард Колледж | Композиции и способы для лечения боли |
| WO2017050872A1 (en) | 2015-09-25 | 2017-03-30 | F. Hoffmann-La Roche Ag | Transamidation employing sortase a in deep eutectic solvents |
| WO2017050874A1 (en) | 2015-09-25 | 2017-03-30 | F. Hoffmann-La Roche Ag | Process for producing thioesters employing a sortase a |
| CN108138158B (zh) | 2015-09-25 | 2021-11-09 | 豪夫迈·罗氏有限公司 | 可溶性分选酶a |
| EP3440013A4 (en) | 2016-04-08 | 2021-03-17 | Massachusetts Institute of Technology | METHOD FOR SPECIFIC PROFILING OF PROTEASE ACTIVITY ON LYMPH NODES |
| US11428689B2 (en) | 2016-05-05 | 2022-08-30 | Massachusetts Institute Of Technology | Methods and uses for remotely triggered protease activity measurements |
| US10738338B2 (en) | 2016-10-18 | 2020-08-11 | The Research Foundation for the State University | Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate |
| US11519905B2 (en) | 2017-04-07 | 2022-12-06 | Massachusetts Institute Of Technology | Methods to spatially profile protease activity in tissue and sections |
| CN108504670B (zh) * | 2018-02-27 | 2022-01-04 | 温州医科大学 | 一种大肠杆菌冷休克助溶型表达质粒的构建方法及其应用 |
| US11054428B2 (en) | 2018-03-05 | 2021-07-06 | Massachusetts Institute Of Technology | Inhalable nanosensors with volatile reporters and uses thereof |
| US11739128B2 (en) | 2018-03-07 | 2023-08-29 | Epicypher, Inc. | Peptide ligase-mediated engineering of recombinant nucleosomes |
| JP7321537B2 (ja) * | 2018-05-22 | 2023-08-07 | ザ・ユニバーシティ・オブ・シドニー | ネイティブケミカルライゲーションを用いる新規フロー合成方法 |
| WO2020068920A2 (en) | 2018-09-25 | 2020-04-02 | Massachusetts Institute Of Technology | Lung protease nanosensors and uses thereof |
| EP3911753A1 (en) | 2019-01-17 | 2021-11-24 | Massachusetts Institute of Technology | Sensors for detecting and imaging of cancer metastasis |
| EP4493217A4 (en) * | 2022-03-17 | 2026-03-11 | Ohio State Innovation Foundation | MEMBRANE TRANSLOCATION AREAS AND ASSOCIATED USES |
| US20250324980A1 (en) * | 2022-05-04 | 2025-10-23 | Ohio State Innovation Foundation | Compositions for delivery of agents into plant cells |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5677274A (en) * | 1993-02-12 | 1997-10-14 | The Government Of The United States As Represented By The Secretary Of The Department Of Health And Human Services | Anthrax toxin fusion proteins and related methods |
| US5723125A (en) | 1995-12-28 | 1998-03-03 | Tanox Biosystems, Inc. | Hybrid with interferon-alpha and an immunoglobulin Fc linked through a non-immunogenic peptide |
| US5770380A (en) | 1996-09-13 | 1998-06-23 | University Of Pittsburgh | Synthetic antibody mimics--multiple peptide loops attached to a molecular scaffold |
| WO1998056807A1 (en) | 1997-06-13 | 1998-12-17 | Gryphon Sciences | Solid phase native chemical ligation of unprotected or n-terminal cysteine protected peptides in aqueous solution |
| GB0030038D0 (en) * | 2000-12-08 | 2001-01-24 | Univ Warwick | Yeast-based assay |
| US20050159349A1 (en) * | 2002-03-05 | 2005-07-21 | Claudio Soto | Apoptogenic-bacteriocins combining broad spectrum antibiotic and selective anti-tumoral activities, and compositions and uses thereof |
| WO2005051976A2 (en) | 2003-11-20 | 2005-06-09 | Ansata Therapeutics, Inc. | Protein and peptide ligation processes and one-step purification processes |
| US7674881B2 (en) | 2005-10-07 | 2010-03-09 | The University Of Chicago | Convergent synthesis of proteins by kinetically controlled ligation |
| WO2007083175A1 (en) * | 2006-01-17 | 2007-07-26 | West China Hospital, Sichuan University | Antiviral bifunctional molecules, methods of construction and methods of treating virus-induced cancer therewith |
| WO2011133704A2 (en) * | 2010-04-20 | 2011-10-27 | Whitehead Institute For Biomedical Researh | Modified polypeptides and proteins and uses thereof |
| EP2663337A4 (en) | 2011-01-10 | 2014-06-11 | Harvard College | METHOD OF DELIVERING AGENTS IN CELLS BY BACTERIAL TOXINE |
| WO2012142659A1 (en) | 2011-04-19 | 2012-10-26 | Baker Idi Heart And Diabetes Institute Holdings Limited | Site-selective modification of proteins |
| US20150044210A1 (en) * | 2012-02-23 | 2015-02-12 | President And Fellows Of Harvard College | Modified microbial toxin receptor for delivering agents into cells |
-
2013
- 2013-05-21 WO PCT/US2013/042118 patent/WO2013177231A1/en not_active Ceased
- 2013-05-21 WO PCT/US2013/042107 patent/WO2013177221A1/en not_active Ceased
- 2013-05-21 EP EP13793070.7A patent/EP2852619A4/en not_active Withdrawn
- 2013-05-21 US US14/402,915 patent/US9731029B2/en active Active
- 2013-05-21 JP JP2015514137A patent/JP2015519344A/ja active Pending
- 2013-05-21 CA CA2874436A patent/CA2874436A1/en not_active Abandoned
- 2013-05-21 US US14/402,856 patent/US9498538B2/en active Active
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