JP2017526353A5 - - Google Patents

Download PDF

Info

Publication number
JP2017526353A5
JP2017526353A5 JP2017506386A JP2017506386A JP2017526353A5 JP 2017526353 A5 JP2017526353 A5 JP 2017526353A5 JP 2017506386 A JP2017506386 A JP 2017506386A JP 2017506386 A JP2017506386 A JP 2017506386A JP 2017526353 A5 JP2017526353 A5 JP 2017526353A5
Authority
JP
Japan
Prior art keywords
probe
sequence
item
nucleic acid
extension products
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2017506386A
Other languages
English (en)
Japanese (ja)
Other versions
JP6803327B2 (ja
JP2017526353A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2015/044065 external-priority patent/WO2016022833A1/en
Publication of JP2017526353A publication Critical patent/JP2017526353A/ja
Publication of JP2017526353A5 publication Critical patent/JP2017526353A5/ja
Application granted granted Critical
Publication of JP6803327B2 publication Critical patent/JP6803327B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2017506386A 2014-08-06 2015-08-06 標的化されたシークエンシングからのデジタル測定値 Active JP6803327B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462034043P 2014-08-06 2014-08-06
US62/034,043 2014-08-06
PCT/US2015/044065 WO2016022833A1 (en) 2014-08-06 2015-08-06 Digital measurements from targeted sequencing

Publications (3)

Publication Number Publication Date
JP2017526353A JP2017526353A (ja) 2017-09-14
JP2017526353A5 true JP2017526353A5 (OSRAM) 2018-08-30
JP6803327B2 JP6803327B2 (ja) 2020-12-23

Family

ID=55264574

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017506386A Active JP6803327B2 (ja) 2014-08-06 2015-08-06 標的化されたシークエンシングからのデジタル測定値

Country Status (7)

Country Link
US (2) US10102337B2 (OSRAM)
EP (1) EP3177740B1 (OSRAM)
JP (1) JP6803327B2 (OSRAM)
CN (1) CN107075581B (OSRAM)
CA (1) CA2957633A1 (OSRAM)
SG (1) SG11201700891SA (OSRAM)
WO (1) WO2016022833A1 (OSRAM)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110016499B (zh) 2011-04-15 2023-11-14 约翰·霍普金斯大学 安全测序系统
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
PL2912468T3 (pl) 2012-10-29 2019-04-30 Univ Johns Hopkins Test papanicolaou pod kątem raka jajnika i endometrium
SG10202009015SA (en) 2012-12-10 2020-10-29 Resolution Bioscience Inc Methods for targeted genomic analysis
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
CN105849264B (zh) 2013-11-13 2019-09-27 纽亘技术公司 用于鉴别重复测序读数的组合物和方法
CA2957633A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
WO2018176007A1 (en) * 2017-03-23 2018-09-27 BioSpyder Technologies, Inc. Modulation of targets in cellular pathways identified by resolution of stochastic gene expression
CA2994601C (en) 2015-08-06 2020-08-25 F. Hoffmann-La Roche Ag Target enrichment by single probe primer extension
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
CA3004435A1 (en) 2015-11-11 2017-05-18 Resolution Bioscience, Inc. High efficiency construction of dna libraries
ES2898088T3 (es) 2016-06-01 2022-03-03 Hoffmann La Roche Inmuno-PETE
CN106048009B (zh) * 2016-06-03 2020-02-18 人和未来生物科技（长沙）有限公司 一种用于超低频基因突变检测的标签接头及其应用
KR101969905B1 (ko) * 2016-06-20 2019-04-17 한국표준과학연구원 염기서열분석을 위한 라이브러리용 프라이머 세트 및 라이브러리 제조방법
US11319594B2 (en) * 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
US10190155B2 (en) 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
CN110352251A (zh) 2017-03-08 2019-10-18 豪夫迈·罗氏有限公司 引物延伸靶标富集及对其的包括同时富集dna和rna的改进
US12297491B2 (en) 2017-03-08 2025-05-13 Roche Sequencing Solutions, Inc. Primer extension target enrichment and improvements thereto including simultaneous enrichment of DNA and RNA
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
BR112020002555A2 (pt) 2017-08-07 2020-08-11 The Johns Hopkins University métodos e materiais para avaliar e tratar câncer
US20200239932A1 (en) * 2017-10-16 2020-07-30 The Regents Of The University Of California Efficient screening library preparation
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CA3202587A1 (en) * 2017-11-06 2019-05-09 Illumina, Inc Nucleic acid indexing techniques
US10985407B2 (en) 2017-11-21 2021-04-20 Samsung Electronics Co., Ltd. All-solid-state secondary battery including anode active material alloyable with lithium and method of charging the same
EP3524695B1 (en) * 2018-02-13 2022-07-20 Personal Genomics S.r.l. Method for the enrichment of genomic regions
US20210310061A1 (en) * 2018-08-17 2021-10-07 Dana-Farber Cancer Institute, Inc. Dna amplification method for probe generation
CN110699426B (zh) * 2019-01-02 2022-01-28 上海臻迪基因科技有限公司 基因目标区域富集方法及试剂盒
CN115066502A (zh) * 2019-09-06 2022-09-16 蜂巢生物技术公司 用于rna-seq分析的方法和系统
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
CA3170345A1 (en) 2020-02-14 2021-08-19 The Johns Hopkins University Methods and materials for assessing nucleic acids
CN113593636B (zh) * 2020-04-30 2024-05-03 深圳市真迈生物科技有限公司 测序结果分析方法、系统及计算机可读存储介质和电子设备
WO2022029449A1 (en) * 2020-08-07 2022-02-10 Oxford Nanopore Technologies Limited Methods of identifying nucleic acid barcodes
CN118139990A (zh) * 2021-10-18 2024-06-04 深圳市真迈生物科技有限公司 测序方法、测序数据处理方法、设备和计算机设备

Family Cites Families (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925065A (en) 1987-01-02 1990-05-15 Helena Laboratories Corporation Dispensing apparatus
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
CA2013317A1 (en) 1989-04-17 1990-10-17 Fred T. Oakes Diagnostic kit, primer composition and their use for replication or detection of nucleic acids
WO1992007951A1 (en) 1990-10-19 1992-05-14 Yoke Wah Kow Quantitation of aldehyde-containing lesions in nucleic acids
US5518900A (en) 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
EP0635023B1 (en) 1992-03-05 2002-02-06 Isis Pharmaceuticals, Inc. Covalently cross-linked oligonucleotides
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US6110709A (en) 1994-03-18 2000-08-29 The General Hospital Corporation Cleaved amplified modified polymorphic sequence detection methods
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
CA2223104A1 (en) 1995-06-07 1996-12-19 Jonathan P. Duvick Pcr-based cdna subtractive cloning method
EP0843735B2 (en) 1995-07-11 2006-12-27 Forfas (trading as Bioresearch Ireland) Glycosylase mediated detection of known nucleotide sequences
US6190865B1 (en) 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
GB9600384D0 (en) 1996-01-09 1996-03-13 Nyfotek As Dna glycosylases
EP2264045B1 (en) 1996-08-14 2015-10-21 Life Technologies Corporation Stable compositions for nucleic acid amplification and sequencing
US6449562B1 (en) 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
IL120337A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Method for joining DNA fragments
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
CA2268368A1 (en) 1997-08-28 1999-03-11 The Perkin-Elmer Corporation Improved detection of mutations in nucleic acids by chemical cleavage
EP1009865A1 (en) 1997-08-29 2000-06-21 Osvaldo J. Lopez Dna methyltransferase genotyping
WO1999022030A1 (en) 1997-10-28 1999-05-06 The Regents Of The University Of California Dna polymorphism identity determination using flow cytometry
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
NZ508358A (en) 1998-04-22 2002-07-26 Univ College Cork Nat Univ Ie A method for checking a DNA fragment (target nucleic acid) for the presence or absence of a mutation
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
GB9817055D0 (en) 1998-08-05 1998-09-30 Medical Res Council Reverse transcription and amplification processes and primers therefore
US6232067B1 (en) 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
JP2002538415A (ja) 1998-12-30 2002-11-12 デイナ−ファーバー キャンサー インスティチュート，インコーポレイテッド 変異走査アレイ、およびその使用方法
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
AU3178200A (en) 1999-03-12 2000-10-04 Amersham Pharmacia Biotech Uk Limited Genetic analysis
WO2000070039A1 (en) 1999-05-17 2000-11-23 Mcgill University METHOD FOR SUBTRACTING cDNAs BY SUPPRESSING THE SYNTHESIS OF SPECIFICALLY TARGETED mRNAs
US6225109B1 (en) 1999-05-27 2001-05-01 Orchid Biosciences, Inc. Genetic analysis device
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
PT1218542E (pt) 1999-09-13 2004-08-31 Nugen Technologies Inc Metodos e composicoes para amplificacao isotermica linear de sequencias polinucleotidicas
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6617442B1 (en) 1999-09-30 2003-09-09 Isis Pharmaceuticals, Inc. Human Rnase H1 and oligonucleotide compositions thereof
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
EP1252172A2 (en) 1999-12-21 2002-10-30 VBC-Genomics Bioscience Research GmbH Compound comprising a peptide moiety and an organo-silane moiety
US7205129B1 (en) 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
ATE455186T1 (de) 2000-06-26 2010-01-15 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
EP1325118B1 (en) 2000-10-05 2016-05-25 Riken Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers.
EP1356104A2 (en) 2000-10-06 2003-10-29 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
IE20000887A1 (en) 2000-11-03 2002-12-11 Univ College Cork Nat Univ Ie Method for the amplification and optional characterisation of nucleic acids
EP1427847B1 (en) 2000-12-13 2010-07-28 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US6893820B1 (en) 2001-01-31 2005-05-17 The Ohio State University Research Foundation Detection of methylated CpG rich sequences diagnostic for malignant cells
BR0205268A (pt) 2001-03-09 2004-11-30 Nugen Technologies Inc Processos e composições para a mplificação de sequências de rna
CA2439074A1 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
WO2002081753A1 (en) 2001-04-04 2002-10-17 Advanced Research & Technology Institute Method for identifying and characterizing individual dna molecules
FR2824335A1 (fr) 2001-05-04 2002-11-08 Bio Merieux Procede de marquage et de fragmentation d'adn
JP3967319B2 (ja) 2001-06-27 2007-08-29 エフ．ホフマン−ラ ロシュ アーゲー ポリヌクレオチド配列のインビトロ組換えのためのウォークスルー技術
WO2003027259A2 (en) 2001-09-26 2003-04-03 Epigenx Pharmaceuticals, Inc. Assays for dna methylation changes
JP2004148509A (ja) 2001-10-04 2004-05-27 Seiko Epson Corp 液体噴射ヘッド
AU2003222269A1 (en) 2002-03-11 2003-09-29 Nugen Technologies, Inc. Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination
US20040023271A1 (en) 2002-03-29 2004-02-05 Nurith Kurn Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification
US6887709B2 (en) 2002-05-09 2005-05-03 Lifescan, Inc. Devices, systems and methods for the containment and use of liquid solutions
AU2003279697A1 (en) 2002-05-17 2004-02-16 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilizaton of nucleic acids
US7108976B2 (en) 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
US7704687B2 (en) 2002-11-15 2010-04-27 The Johns Hopkins University Digital karyotyping
US7414117B2 (en) 2002-12-26 2008-08-19 Ngk Insulators, Ltd. Nucleotide derivative and DNA microarray
EP1594980B2 (en) 2003-01-29 2017-08-16 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
EP1613778B1 (en) 2003-04-14 2014-11-26 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
WO2005038427A2 (en) 2003-06-05 2005-04-28 Medical Gene Center Ltd. Rapid identification of viruses of the upper respiratory tract infection including sars causing coronus viruses
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
JP2007524407A (ja) 2003-12-29 2007-08-30 ニューゲン テクノロジーズ， インコーポレイテッド 核酸のメチル化状態を分析するための方法、ならびに核酸の断片化、標識化および固定化のための方法
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
US7361468B2 (en) 2004-07-02 2008-04-22 Affymetrix, Inc. Methods for genotyping polymorphisms in humans
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7276720B2 (en) 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060024678A1 (en) 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
WO2006055521A2 (en) 2004-11-16 2006-05-26 Helicos Biosciences Corporation Tirf single molecule analysis and method of sequencing nucleic acids
WO2006081222A2 (en) 2005-01-25 2006-08-03 Compass Genetics, Llc. Isothermal dna amplification
US7462468B1 (en) 2005-01-28 2008-12-09 Pacific Biosciences Of California, Inc. DNA intercalating agents and methods of use
EP1844328A4 (en) 2005-01-31 2009-09-16 Pacific Biosciences California USE OF REVERSIBLE EXTENSION TERMINATOR IN NUCLEIC ACID SEQUENCING
US20060286566A1 (en) 2005-02-03 2006-12-21 Helicos Biosciences Corporation Detecting apparent mutations in nucleic acid sequences
US20060240451A1 (en) 2005-02-09 2006-10-26 Epicentre Technologies Compositions and methods employing 5' phosphate-dependent nucleic acid exonucleases
WO2007018601A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
WO2007019444A2 (en) 2005-08-05 2007-02-15 Euclid Diagnostics Llc Subtractive separation and amplification of non-ribosomal transcribed rna (nrrna)
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP1957667A1 (en) 2005-11-15 2008-08-20 Solexa Ltd. Method of target enrichment
WO2008115185A2 (en) 2006-04-24 2008-09-25 Nimblegen Systems, Inc. Use of microarrays for genomic representation selection
US20080269476A1 (en) 2006-04-26 2008-10-30 Helicos Biosciences Corporation Molecules and methods for nucleic acid sequencing
CA2652052A1 (en) 2006-05-16 2007-11-29 Nugen Technologies, Inc. Nucleic acid separation and purification method based on reversible charge interactions
EP2038429B1 (en) 2006-06-30 2013-08-21 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
US9328378B2 (en) 2006-07-31 2016-05-03 Illumina Cambridge Limited Method of library preparation avoiding the formation of adaptor dimers
WO2008033442A2 (en) 2006-09-12 2008-03-20 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for performing low background multiplex nucleic acid amplification reactions
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8506908B2 (en) 2007-03-09 2013-08-13 Vantix Holdings Limited Electrochemical detection system
WO2008137661A1 (en) 2007-05-03 2008-11-13 Helicos Biosciences Corporation Methods and compositions for sequencing a nucleic acid
US9248076B2 (en) 2007-05-16 2016-02-02 Mystic Pharmaceuticals, Inc. Dose dispensing containers
AU2008261935B2 (en) 2007-06-06 2013-05-02 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
CL2008001682A1 (es) * 2007-06-08 2008-12-12 Monsanto Technology Llc Metodos para el mejoramiento de plantas mediante el uso de informacion directa de secuencias de acidos nucleicos.
US7901889B2 (en) 2007-07-26 2011-03-08 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
WO2009102896A2 (en) 2008-02-12 2009-08-20 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
GB0804690D0 (en) 2008-03-13 2008-04-16 Netherlands Cancer Inst The Method
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
WO2009120372A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
WO2010003153A2 (en) 2008-07-03 2010-01-07 Life Technologies Corporation Methylation analysis of mate pairs
JP5221248B2 (ja) 2008-08-26 2013-06-26 株式会社日立ハイテクノロジーズ 高発現遺伝子由来のｃＤＮＡクローンの含有率を低減させたｃＤＮＡライブラリーの作製方法
WO2010030683A1 (en) 2008-09-09 2010-03-18 Rosetta Inpharmatics Llc Methods of generating gene specific libraries
WO2010039991A2 (en) 2008-10-02 2010-04-08 The Texas A&M University System Method of generating informative dna templates for high-throughput sequencing applications
GB2465986A (en) 2008-12-04 2010-06-09 Angeletti P Ist Richerche Bio Method of generating diversity in polynucleotide sequences
WO2010064893A1 (en) 2008-12-04 2010-06-10 Keygene N.V. Method for the reduction of repetitive sequences in adapter-ligated restriction fragments
CA2750547C (en) 2009-01-22 2018-07-03 Quanta Biosciences Methods for enrichment of selected rna molecules
AU2010210550C1 (en) 2009-02-06 2016-06-09 Northwestern University Burstable liquid packaging and uses thereof
US8691509B2 (en) 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
WO2010129937A2 (en) 2009-05-08 2010-11-11 Life Technologies Corporation Methods for detecting genetic variations in dna samples
EP2272976A1 (en) 2009-07-06 2011-01-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for differentiation of polynucleotide strands
GB0912909D0 (en) 2009-07-23 2009-08-26 Olink Genomics Ab Probes for specific analysis of nucleic acids
WO2011019964A1 (en) 2009-08-12 2011-02-17 Nugen Technologies, Inc. Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid
WO2011032053A1 (en) 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
GB2487341A (en) 2009-11-02 2012-07-18 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence selection and amplification
ITRM20100293A1 (it) 2010-05-31 2011-12-01 Consiglio Nazionale Ricerche Metodo per la preparazione e amplificazione di librerie rappresentative di cdna per il sequenziamento massivo, loro uso, kit e cartucce per kit di automazione
WO2011156529A2 (en) 2010-06-08 2011-12-15 Nugen Technologies, Inc. Methods and composition for multiplex sequencing
GB201012748D0 (en) 2010-07-29 2010-09-15 Univ St Andrews Improved RACE
ES2690753T3 (es) 2010-09-21 2018-11-22 Agilent Technologies, Inc. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
EP4328321A3 (en) 2010-10-22 2024-05-29 Cold Spring Harbor Laboratory Varietal counting of nucleic acids for obtaining genomic copy number information
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
AU2011323107B2 (en) 2010-11-05 2015-09-10 Illumina, Inc. Linking sequence reads using paired code tags
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
SG194745A1 (en) * 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
WO2013059740A1 (en) 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
CN104093890B (zh) 2012-01-26 2016-04-20 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
WO2013130512A2 (en) 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
US9862995B2 (en) 2012-03-13 2018-01-09 Abhijit Ajit Patel Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
CN104736722B (zh) 2012-05-21 2018-08-07 斯克利普斯研究所 样品制备方法
US9957549B2 (en) 2012-06-18 2018-05-01 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US11261494B2 (en) 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
EP2885445A4 (en) * 2012-08-15 2016-04-13 Natera Inc METHOD AND COMPOSITIONS FOR CONTAMINATING REDUCTION OF GENETIC LIBRARIES
ES2769241T5 (es) 2012-09-04 2023-05-30 Guardant Health Inc Sistemas y métodos para detectar variación en el número de copias
CA3043574C (en) 2012-11-26 2023-09-19 The University Of Toledo Methods for standardized sequencing of nucleic acids and uses thereof
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
WO2015057899A1 (en) 2013-10-15 2015-04-23 Mystic Pharmaceuticals, Inc. Controllable rate turbulating nozzle
CN105849264B (zh) * 2013-11-13 2019-09-27 纽亘技术公司 用于鉴别重复测序读数的组合物和方法
GB201320542D0 (en) 2013-11-21 2014-01-01 Randox Lab Ltd Assay fluid delivery system
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CA2957633A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
US9527077B2 (en) 2015-01-29 2016-12-27 David W. Wright Diagnostic cartridge, fluid storage and delivery apparatus therefor and methods of construction thereof

Similar Documents

Publication Publication Date Title
JP2017526353A5 (OSRAM)
US11795501B2 (en) Methods for next generation genome walking and related compositions and kits
Sengupta et al. Molecular detection and identification of influenza viruses by oligonucleotide microarray hybridization
Shirima et al. Absolute quantification of cassava brown streak virus mRNA by real-time qPCR
CN107075581A (zh) 由靶向测序进行数字测量
JP2013531983A (ja) 多重生物検出のための核酸ならびにその使用および製造方法
JP2013539970A (ja) 分子計数による対立遺伝子呼び出しの信頼度の増加
No et al. Comparison of targeted next-generation sequencing for whole-genome sequencing of Hantaan orthohantavirus in Apodemus agrarius lung tissues
KR102242192B1 (ko) 미세 조정된 초특정 핵산 하이브리다이제이션 프로브
Malandraki et al. Simultaneous detection of three pome fruit tree viruses by one-step multiplex quantitative RT-PCR
US20080261198A1 (en) Diagnostic Primers and Method for Detecting Avian Influenza Virus Subtype H5 and H5n1
JP7528911B2 (ja) Ｄｎａライブラリーの作製方法及びｄｎａライブラリーを用いたゲノムｄｎａ解析方法
CN108517371B (zh) 用于二倍体d基因组棉种或四倍体棉种鉴定的引物及方法
Nemoto et al. Molecular characterisation of equine group A rotaviruses in Ireland (2011–2015)
CN102719543B (zh) 利用核苷酸化学分子式鉴定植物品种的方法
LI Li j
Cong et al. A SYBR Green-based real-time RT-PCR assay to differentiate the H1N1 influenza virus lineages
CA3179579A1 (en) Pathogen diagnostic test
Xiao et al. Design and validation of a universal influenza virus enrichment probe set and its utility in deep sequence analysis of primary cloacal swab surveillance samples of wild birds
CN106967833B (zh) 用于二倍体a基因组棉种和/或四倍体棉种鉴定的引物及其pcr鉴定方法
US20200071776A1 (en) Set of random primers and method for preparing dna library using the same
CN110195117A (zh) 检测分枝杆菌的方法及其试剂盒
CN116287107A (zh) 一种核酸探针及其使用方法
CN118879945B (zh) 一种疫苗毒株鉴定的引物探针组合、试剂盒及应用
CN107142321B (zh) 一种蒙古扁桃特异性微卫星位点及其应用