JP6803327B2 - 標的化されたシークエンシングからのデジタル測定値 - Google Patents
標的化されたシークエンシングからのデジタル測定値 Download PDFInfo
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- JP6803327B2 JP6803327B2 JP2017506386A JP2017506386A JP6803327B2 JP 6803327 B2 JP6803327 B2 JP 6803327B2 JP 2017506386 A JP2017506386 A JP 2017506386A JP 2017506386 A JP2017506386 A JP 2017506386A JP 6803327 B2 JP6803327 B2 JP 6803327B2
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/10—Sequence alignment; Homology search
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Evolutionary Biology (AREA)
- Medical Informatics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Theoretical Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Databases & Information Systems (AREA)
- Bioethics (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462034043P | 2014-08-06 | 2014-08-06 | |
| US62/034,043 | 2014-08-06 | ||
| PCT/US2015/044065 WO2016022833A1 (en) | 2014-08-06 | 2015-08-06 | Digital measurements from targeted sequencing |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017526353A JP2017526353A (ja) | 2017-09-14 |
| JP2017526353A5 JP2017526353A5 (OSRAM) | 2018-08-30 |
| JP6803327B2 true JP6803327B2 (ja) | 2020-12-23 |
Family
ID=55264574
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017506386A Active JP6803327B2 (ja) | 2014-08-06 | 2015-08-06 | 標的化されたシークエンシングからのデジタル測定値 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10102337B2 (OSRAM) |
| EP (1) | EP3177740B1 (OSRAM) |
| JP (1) | JP6803327B2 (OSRAM) |
| CN (1) | CN107075581B (OSRAM) |
| CA (1) | CA2957633A1 (OSRAM) |
| SG (1) | SG11201700891SA (OSRAM) |
| WO (1) | WO2016022833A1 (OSRAM) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110016499B (zh) | 2011-04-15 | 2023-11-14 | 约翰·霍普金斯大学 | 安全测序系统 |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| PL2912468T3 (pl) | 2012-10-29 | 2019-04-30 | Univ Johns Hopkins | Test papanicolaou pod kątem raka jajnika i endometrium |
| SG10202009015SA (en) | 2012-12-10 | 2020-10-29 | Resolution Bioscience Inc | Methods for targeted genomic analysis |
| EP2971130A4 (en) | 2013-03-15 | 2016-10-05 | Nugen Technologies Inc | SEQUENTIAL SEQUENCING |
| CN105849264B (zh) | 2013-11-13 | 2019-09-27 | 纽亘技术公司 | 用于鉴别重复测序读数的组合物和方法 |
| CA2957633A1 (en) | 2014-08-06 | 2016-02-11 | Nugen Technologies, Inc. | Digital measurements from targeted sequencing |
| US20160053301A1 (en) | 2014-08-22 | 2016-02-25 | Clearfork Bioscience, Inc. | Methods for quantitative genetic analysis of cell free dna |
| WO2018176007A1 (en) * | 2017-03-23 | 2018-09-27 | BioSpyder Technologies, Inc. | Modulation of targets in cellular pathways identified by resolution of stochastic gene expression |
| CA2994601C (en) | 2015-08-06 | 2020-08-25 | F. Hoffmann-La Roche Ag | Target enrichment by single probe primer extension |
| WO2017027653A1 (en) | 2015-08-11 | 2017-02-16 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| CA3004435A1 (en) | 2015-11-11 | 2017-05-18 | Resolution Bioscience, Inc. | High efficiency construction of dna libraries |
| ES2898088T3 (es) | 2016-06-01 | 2022-03-03 | Hoffmann La Roche | Inmuno-PETE |
| CN106048009B (zh) * | 2016-06-03 | 2020-02-18 | 人和未来生物科技（长沙）有限公司 | 一种用于超低频基因突变检测的标签接头及其应用 |
| KR101969905B1 (ko) * | 2016-06-20 | 2019-04-17 | 한국표준과학연구원 | 염기서열분석을 위한 라이브러리용 프라이머 세트 및 라이브러리 제조방법 |
| US11319594B2 (en) * | 2016-08-25 | 2022-05-03 | Resolution Bioscience, Inc. | Methods for the detection of genomic copy changes in DNA samples |
| US10190155B2 (en) | 2016-10-14 | 2019-01-29 | Nugen Technologies, Inc. | Molecular tag attachment and transfer |
| CN110352251A (zh) | 2017-03-08 | 2019-10-18 | 豪夫迈·罗氏有限公司 | 引物延伸靶标富集及对其的包括同时富集dna和rna的改进 |
| US12297491B2 (en) | 2017-03-08 | 2025-05-13 | Roche Sequencing Solutions, Inc. | Primer extension target enrichment and improvements thereto including simultaneous enrichment of DNA and RNA |
| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| BR112020002555A2 (pt) | 2017-08-07 | 2020-08-11 | The Johns Hopkins University | métodos e materiais para avaliar e tratar câncer |
| US20200239932A1 (en) * | 2017-10-16 | 2020-07-30 | The Regents Of The University Of California | Efficient screening library preparation |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| CA3202587A1 (en) * | 2017-11-06 | 2019-05-09 | Illumina, Inc | Nucleic acid indexing techniques |
| US10985407B2 (en) | 2017-11-21 | 2021-04-20 | Samsung Electronics Co., Ltd. | All-solid-state secondary battery including anode active material alloyable with lithium and method of charging the same |
| EP3524695B1 (en) * | 2018-02-13 | 2022-07-20 | Personal Genomics S.r.l. | Method for the enrichment of genomic regions |
| US20210310061A1 (en) * | 2018-08-17 | 2021-10-07 | Dana-Farber Cancer Institute, Inc. | Dna amplification method for probe generation |
| CN110699426B (zh) * | 2019-01-02 | 2022-01-28 | 上海臻迪基因科技有限公司 | 基因目标区域富集方法及试剂盒 |
| CN115066502A (zh) * | 2019-09-06 | 2022-09-16 | 蜂巢生物技术公司 | 用于rna-seq分析的方法和系统 |
| US12059674B2 (en) | 2020-02-03 | 2024-08-13 | Tecan Genomics, Inc. | Reagent storage system |
| CA3170345A1 (en) | 2020-02-14 | 2021-08-19 | The Johns Hopkins University | Methods and materials for assessing nucleic acids |
| CN113593636B (zh) * | 2020-04-30 | 2024-05-03 | 深圳市真迈生物科技有限公司 | 测序结果分析方法、系统及计算机可读存储介质和电子设备 |
| WO2022029449A1 (en) * | 2020-08-07 | 2022-02-10 | Oxford Nanopore Technologies Limited | Methods of identifying nucleic acid barcodes |
| CN118139990A (zh) * | 2021-10-18 | 2024-06-04 | 深圳市真迈生物科技有限公司 | 测序方法、测序数据处理方法、设备和计算机设备 |
Family Cites Families (152)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4925065A (en) | 1987-01-02 | 1990-05-15 | Helena Laboratories Corporation | Dispensing apparatus |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| CA2013317A1 (en) | 1989-04-17 | 1990-10-17 | Fred T. Oakes | Diagnostic kit, primer composition and their use for replication or detection of nucleic acids |
| WO1992007951A1 (en) | 1990-10-19 | 1992-05-14 | Yoke Wah Kow | Quantitation of aldehyde-containing lesions in nucleic acids |
| US5518900A (en) | 1993-01-15 | 1996-05-21 | Molecular Tool, Inc. | Method for generating single-stranded DNA molecules |
| EP0635023B1 (en) | 1992-03-05 | 2002-02-06 | Isis Pharmaceuticals, Inc. | Covalently cross-linked oligonucleotides |
| CA2140081C (en) | 1994-01-13 | 2008-04-01 | Dean L. Engelhardt | Process, construct and conjugate for producing multiple nucleic acid copies |
| US6110709A (en) | 1994-03-18 | 2000-08-29 | The General Hospital Corporation | Cleaved amplified modified polymorphic sequence detection methods |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| CA2223104A1 (en) | 1995-06-07 | 1996-12-19 | Jonathan P. Duvick | Pcr-based cdna subtractive cloning method |
| EP0843735B2 (en) | 1995-07-11 | 2006-12-27 | Forfas (trading as Bioresearch Ireland) | Glycosylase mediated detection of known nucleotide sequences |
| US6190865B1 (en) | 1995-09-27 | 2001-02-20 | Epicentre Technologies Corporation | Method for characterizing nucleic acid molecules |
| GB9600384D0 (en) | 1996-01-09 | 1996-03-13 | Nyfotek As | Dna glycosylases |
| EP2264045B1 (en) | 1996-08-14 | 2015-10-21 | Life Technologies Corporation | Stable compositions for nucleic acid amplification and sequencing |
| US6449562B1 (en) | 1996-10-10 | 2002-09-10 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
| IL120337A0 (en) | 1997-02-27 | 1997-06-10 | Gesher Israel Advanced Biotecs | Method for joining DNA fragments |
| WO1998044151A1 (en) | 1997-04-01 | 1998-10-08 | Glaxo Group Limited | Method of nucleic acid amplification |
| CA2268368A1 (en) | 1997-08-28 | 1999-03-11 | The Perkin-Elmer Corporation | Improved detection of mutations in nucleic acids by chemical cleavage |
| EP1009865A1 (en) | 1997-08-29 | 2000-06-21 | Osvaldo J. Lopez | Dna methyltransferase genotyping |
| WO1999022030A1 (en) | 1997-10-28 | 1999-05-06 | The Regents Of The University Of California | Dna polymorphism identity determination using flow cytometry |
| US6365346B1 (en) | 1998-02-18 | 2002-04-02 | Dade Behring Inc. | Quantitative determination of nucleic acid amplification products |
| NZ508358A (en) | 1998-04-22 | 2002-07-26 | Univ College Cork Nat Univ Ie | A method for checking a DNA fragment (target nucleic acid) for the presence or absence of a mutation |
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| GB0002310D0 (en) | 2000-02-01 | 2000-03-22 | Solexa Ltd | Polynucleotide sequencing |
| GB9817055D0 (en) | 1998-08-05 | 1998-09-30 | Medical Res Council | Reverse transcription and amplification processes and primers therefore |
| US6232067B1 (en) | 1998-08-17 | 2001-05-15 | The Perkin-Elmer Corporation | Adapter directed expression analysis |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| JP2002538415A (ja) | 1998-12-30 | 2002-11-12 | デイナ−ファーバー キャンサー インスティチュート，インコーポレイテッド | 変異走査アレイ、およびその使用方法 |
| US7074556B2 (en) | 1999-03-02 | 2006-07-11 | Invitrogen Corporation | cDNA synthesis improvements |
| AU3178200A (en) | 1999-03-12 | 2000-10-04 | Amersham Pharmacia Biotech Uk Limited | Genetic analysis |
| WO2000070039A1 (en) | 1999-05-17 | 2000-11-23 | Mcgill University | METHOD FOR SUBTRACTING cDNAs BY SUPPRESSING THE SYNTHESIS OF SPECIFICALLY TARGETED mRNAs |
| US6225109B1 (en) | 1999-05-27 | 2001-05-01 | Orchid Biosciences, Inc. | Genetic analysis device |
| US7501245B2 (en) | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
| PT1218542E (pt) | 1999-09-13 | 2004-08-31 | Nugen Technologies Inc | Metodos e composicoes para amplificacao isotermica linear de sequencias polinucleotidicas |
| US7211390B2 (en) | 1999-09-16 | 2007-05-01 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US6617442B1 (en) | 1999-09-30 | 2003-09-09 | Isis Pharmaceuticals, Inc. | Human Rnase H1 and oligonucleotide compositions thereof |
| US6958225B2 (en) | 1999-10-27 | 2005-10-25 | Affymetrix, Inc. | Complexity management of genomic DNA |
| EP1252172A2 (en) | 1999-12-21 | 2002-10-30 | VBC-Genomics Bioscience Research GmbH | Compound comprising a peptide moiety and an organo-silane moiety |
| US7205129B1 (en) | 2000-02-28 | 2007-04-17 | Qiagen Gmbh | Method for reducing artifacts in nucleic acid amplification |
| US6936702B2 (en) | 2000-06-07 | 2005-08-30 | Li-Cor, Inc. | Charge-switch nucleotides |
| ATE455186T1 (de) | 2000-06-26 | 2010-01-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
| EP1325118B1 (en) | 2000-10-05 | 2016-05-25 | Riken | Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers. |
| EP1356104A2 (en) | 2000-10-06 | 2003-10-29 | Nugen Technologies, Inc. | Methods and probes for detection and/or quantification of nucleic acid sequences |
| IE20000887A1 (en) | 2000-11-03 | 2002-12-11 | Univ College Cork Nat Univ Ie | Method for the amplification and optional characterisation of nucleic acids |
| EP1427847B1 (en) | 2000-12-13 | 2010-07-28 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
| US6893820B1 (en) | 2001-01-31 | 2005-05-17 | The Ohio State University Research Foundation | Detection of methylated CpG rich sequences diagnostic for malignant cells |
| BR0205268A (pt) | 2001-03-09 | 2004-11-30 | Nugen Technologies Inc | Processos e composições para a mplificação de sequências de rna |
| CA2439074A1 (en) | 2001-03-09 | 2002-09-19 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
| WO2002081753A1 (en) | 2001-04-04 | 2002-10-17 | Advanced Research & Technology Institute | Method for identifying and characterizing individual dna molecules |
| FR2824335A1 (fr) | 2001-05-04 | 2002-11-08 | Bio Merieux | Procede de marquage et de fragmentation d'adn |
| JP3967319B2 (ja) | 2001-06-27 | 2007-08-29 | エフ．ホフマン−ラ ロシュ アーゲー | ポリヌクレオチド配列のインビトロ組換えのためのウォークスルー技術 |
| WO2003027259A2 (en) | 2001-09-26 | 2003-04-03 | Epigenx Pharmaceuticals, Inc. | Assays for dna methylation changes |
| JP2004148509A (ja) | 2001-10-04 | 2004-05-27 | Seiko Epson Corp | 液体噴射ヘッド |
| AU2003222269A1 (en) | 2002-03-11 | 2003-09-29 | Nugen Technologies, Inc. | Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination |
| US20040023271A1 (en) | 2002-03-29 | 2004-02-05 | Nurith Kurn | Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification |
| US6887709B2 (en) | 2002-05-09 | 2005-05-03 | Lifescan, Inc. | Devices, systems and methods for the containment and use of liquid solutions |
| AU2003279697A1 (en) | 2002-05-17 | 2004-02-16 | Nugen Technologies, Inc. | Methods for fragmentation, labeling and immobilizaton of nucleic acids |
| US7108976B2 (en) | 2002-06-17 | 2006-09-19 | Affymetrix, Inc. | Complexity management of genomic DNA by locus specific amplification |
| US7704687B2 (en) | 2002-11-15 | 2010-04-27 | The Johns Hopkins University | Digital karyotyping |
| US7414117B2 (en) | 2002-12-26 | 2008-08-19 | Ngk Insulators, Ltd. | Nucleotide derivative and DNA microarray |
| EP1594980B2 (en) | 2003-01-29 | 2017-08-16 | 454 Life Sciences Corporation | Bead emulsion nucleic acid amplification |
| US7745116B2 (en) | 2003-04-08 | 2010-06-29 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| EP1613778B1 (en) | 2003-04-14 | 2014-11-26 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
| WO2005038427A2 (en) | 2003-06-05 | 2005-04-28 | Medical Gene Center Ltd. | Rapid identification of viruses of the upper respiratory tract infection including sars causing coronus viruses |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| JP2007524407A (ja) | 2003-12-29 | 2007-08-30 | ニューゲン テクノロジーズ， インコーポレイテッド | 核酸のメチル化状態を分析するための方法、ならびに核酸の断片化、標識化および固定化のための方法 |
| US7462452B2 (en) | 2004-04-30 | 2008-12-09 | Pacific Biosciences Of California, Inc. | Field-switch sequencing |
| US7361468B2 (en) | 2004-07-02 | 2008-04-22 | Affymetrix, Inc. | Methods for genotyping polymorphisms in humans |
| US20060024711A1 (en) | 2004-07-02 | 2006-02-02 | Helicos Biosciences Corporation | Methods for nucleic acid amplification and sequence determination |
| US7276720B2 (en) | 2004-07-19 | 2007-10-02 | Helicos Biosciences Corporation | Apparatus and methods for analyzing samples |
| US20060024678A1 (en) | 2004-07-28 | 2006-02-02 | Helicos Biosciences Corporation | Use of single-stranded nucleic acid binding proteins in sequencing |
| US20060062531A1 (en) | 2004-09-17 | 2006-03-23 | Stephen Turner | Fabrication of optical confinements |
| US7170050B2 (en) | 2004-09-17 | 2007-01-30 | Pacific Biosciences Of California, Inc. | Apparatus and methods for optical analysis of molecules |
| WO2006055521A2 (en) | 2004-11-16 | 2006-05-26 | Helicos Biosciences Corporation | Tirf single molecule analysis and method of sequencing nucleic acids |
| WO2006081222A2 (en) | 2005-01-25 | 2006-08-03 | Compass Genetics, Llc. | Isothermal dna amplification |
| US7462468B1 (en) | 2005-01-28 | 2008-12-09 | Pacific Biosciences Of California, Inc. | DNA intercalating agents and methods of use |
| EP1844328A4 (en) | 2005-01-31 | 2009-09-16 | Pacific Biosciences California | USE OF REVERSIBLE EXTENSION TERMINATOR IN NUCLEIC ACID SEQUENCING |
| US20060286566A1 (en) | 2005-02-03 | 2006-12-21 | Helicos Biosciences Corporation | Detecting apparent mutations in nucleic acid sequences |
| US20060240451A1 (en) | 2005-02-09 | 2006-10-26 | Epicentre Technologies | Compositions and methods employing 5' phosphate-dependent nucleic acid exonucleases |
| WO2007018601A1 (en) | 2005-08-02 | 2007-02-15 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction |
| WO2007019444A2 (en) | 2005-08-05 | 2007-02-15 | Euclid Diagnostics Llc | Subtractive separation and amplification of non-ribosomal transcribed rna (nrrna) |
| WO2007030759A2 (en) | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| EP1957667A1 (en) | 2005-11-15 | 2008-08-20 | Solexa Ltd. | Method of target enrichment |
| WO2008115185A2 (en) | 2006-04-24 | 2008-09-25 | Nimblegen Systems, Inc. | Use of microarrays for genomic representation selection |
| US20080269476A1 (en) | 2006-04-26 | 2008-10-30 | Helicos Biosciences Corporation | Molecules and methods for nucleic acid sequencing |
| CA2652052A1 (en) | 2006-05-16 | 2007-11-29 | Nugen Technologies, Inc. | Nucleic acid separation and purification method based on reversible charge interactions |
| EP2038429B1 (en) | 2006-06-30 | 2013-08-21 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
| US9328378B2 (en) | 2006-07-31 | 2016-05-03 | Illumina Cambridge Limited | Method of library preparation avoiding the formation of adaptor dimers |
| WO2008033442A2 (en) | 2006-09-12 | 2008-03-20 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for performing low background multiplex nucleic acid amplification reactions |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| WO2008093098A2 (en) | 2007-02-02 | 2008-08-07 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| US8506908B2 (en) | 2007-03-09 | 2013-08-13 | Vantix Holdings Limited | Electrochemical detection system |
| WO2008137661A1 (en) | 2007-05-03 | 2008-11-13 | Helicos Biosciences Corporation | Methods and compositions for sequencing a nucleic acid |
| US9248076B2 (en) | 2007-05-16 | 2016-02-02 | Mystic Pharmaceuticals, Inc. | Dose dispensing containers |
| AU2008261935B2 (en) | 2007-06-06 | 2013-05-02 | Pacific Biosciences Of California, Inc. | Methods and processes for calling bases in sequence by incorporation methods |
| CL2008001682A1 (es) * | 2007-06-08 | 2008-12-12 | Monsanto Technology Llc | Metodos para el mejoramiento de plantas mediante el uso de informacion directa de secuencias de acidos nucleicos. |
| US7901889B2 (en) | 2007-07-26 | 2011-03-08 | Pacific Biosciences Of California, Inc. | Molecular redundant sequencing |
| EP2053132A1 (en) | 2007-10-23 | 2009-04-29 | Roche Diagnostics GmbH | Enrichment and sequence analysis of geomic regions |
| WO2009102896A2 (en) | 2008-02-12 | 2009-08-20 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
| GB0804690D0 (en) | 2008-03-13 | 2008-04-16 | Netherlands Cancer Inst The | Method |
| WO2009117698A2 (en) | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
| WO2009120372A2 (en) | 2008-03-28 | 2009-10-01 | Pacific Biosciences Of California, Inc. | Compositions and methods for nucleic acid sequencing |
| WO2009120374A2 (en) | 2008-03-28 | 2009-10-01 | Pacific Biosciences Of California, Inc. | Methods and compositions for nucleic acid sample preparation |
| WO2010003153A2 (en) | 2008-07-03 | 2010-01-07 | Life Technologies Corporation | Methylation analysis of mate pairs |
| JP5221248B2 (ja) | 2008-08-26 | 2013-06-26 | 株式会社日立ハイテクノロジーズ | 高発現遺伝子由来のｃＤＮＡクローンの含有率を低減させたｃＤＮＡライブラリーの作製方法 |
| WO2010030683A1 (en) | 2008-09-09 | 2010-03-18 | Rosetta Inpharmatics Llc | Methods of generating gene specific libraries |
| WO2010039991A2 (en) | 2008-10-02 | 2010-04-08 | The Texas A&M University System | Method of generating informative dna templates for high-throughput sequencing applications |
| GB2465986A (en) | 2008-12-04 | 2010-06-09 | Angeletti P Ist Richerche Bio | Method of generating diversity in polynucleotide sequences |
| WO2010064893A1 (en) | 2008-12-04 | 2010-06-10 | Keygene N.V. | Method for the reduction of repetitive sequences in adapter-ligated restriction fragments |
| CA2750547C (en) | 2009-01-22 | 2018-07-03 | Quanta Biosciences | Methods for enrichment of selected rna molecules |
| AU2010210550C1 (en) | 2009-02-06 | 2016-06-09 | Northwestern University | Burstable liquid packaging and uses thereof |
| US8691509B2 (en) | 2009-04-02 | 2014-04-08 | Fluidigm Corporation | Multi-primer amplification method for barcoding of target nucleic acids |
| WO2010129937A2 (en) | 2009-05-08 | 2010-11-11 | Life Technologies Corporation | Methods for detecting genetic variations in dna samples |
| EP2272976A1 (en) | 2009-07-06 | 2011-01-12 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Method for differentiation of polynucleotide strands |
| GB0912909D0 (en) | 2009-07-23 | 2009-08-26 | Olink Genomics Ab | Probes for specific analysis of nucleic acids |
| WO2011019964A1 (en) | 2009-08-12 | 2011-02-17 | Nugen Technologies, Inc. | Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid |
| WO2011032053A1 (en) | 2009-09-11 | 2011-03-17 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
| GB2487341A (en) | 2009-11-02 | 2012-07-18 | Nugen Technologies Inc | Compositions and methods for targeted nucleic acid sequence selection and amplification |
| ITRM20100293A1 (it) | 2010-05-31 | 2011-12-01 | Consiglio Nazionale Ricerche | Metodo per la preparazione e amplificazione di librerie rappresentative di cdna per il sequenziamento massivo, loro uso, kit e cartucce per kit di automazione |
| WO2011156529A2 (en) | 2010-06-08 | 2011-12-15 | Nugen Technologies, Inc. | Methods and composition for multiplex sequencing |
| GB201012748D0 (en) | 2010-07-29 | 2010-09-15 | Univ St Andrews | Improved RACE |
| ES2690753T3 (es) | 2010-09-21 | 2018-11-22 | Agilent Technologies, Inc. | Aumento de la confianza en las identificaciones de alelos con el recuento molecular |
| EP4328321A3 (en) | 2010-10-22 | 2024-05-29 | Cold Spring Harbor Laboratory | Varietal counting of nucleic acids for obtaining genomic copy number information |
| US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
| AU2011323107B2 (en) | 2010-11-05 | 2015-09-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
| WO2012103154A1 (en) | 2011-01-24 | 2012-08-02 | Nugen Technologies, Inc. | Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations |
| US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
| SG194745A1 (en) * | 2011-05-20 | 2013-12-30 | Fluidigm Corp | Nucleic acid encoding reactions |
| SG10201510189WA (en) | 2011-10-19 | 2016-01-28 | Nugen Technologies Inc | Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing |
| WO2013059740A1 (en) | 2011-10-21 | 2013-04-25 | Foundation Medicine, Inc. | Novel alk and ntrk1 fusion molecules and uses thereof |
| CN104093890B (zh) | 2012-01-26 | 2016-04-20 | 纽亘技术公司 | 用于靶向核酸序列富集和高效文库产生的组合物和方法 |
| WO2013130512A2 (en) | 2012-02-27 | 2013-09-06 | The University Of North Carolina At Chapel Hill | Methods and uses for molecular tags |
| US9862995B2 (en) | 2012-03-13 | 2018-01-09 | Abhijit Ajit Patel | Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing |
| US8209130B1 (en) | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
| CN104736722B (zh) | 2012-05-21 | 2018-08-07 | 斯克利普斯研究所 | 样品制备方法 |
| US9957549B2 (en) | 2012-06-18 | 2018-05-01 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US11261494B2 (en) | 2012-06-21 | 2022-03-01 | The Chinese University Of Hong Kong | Method of measuring a fractional concentration of tumor DNA |
| EP2885445A4 (en) * | 2012-08-15 | 2016-04-13 | Natera Inc | METHOD AND COMPOSITIONS FOR CONTAMINATING REDUCTION OF GENETIC LIBRARIES |
| ES2769241T5 (es) | 2012-09-04 | 2023-05-30 | Guardant Health Inc | Sistemas y métodos para detectar variación en el número de copias |
| CA3043574C (en) | 2012-11-26 | 2023-09-19 | The University Of Toledo | Methods for standardized sequencing of nucleic acids and uses thereof |
| US20140274729A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Methods, compositions and kits for generation of stranded rna or dna libraries |
| EP2971130A4 (en) | 2013-03-15 | 2016-10-05 | Nugen Technologies Inc | SEQUENTIAL SEQUENCING |
| ES2857908T3 (es) | 2013-08-28 | 2021-09-29 | Becton Dickinson Co | Análisis masivamente paralelo de células individuales |
| WO2015057899A1 (en) | 2013-10-15 | 2015-04-23 | Mystic Pharmaceuticals, Inc. | Controllable rate turbulating nozzle |
| CN105849264B (zh) * | 2013-11-13 | 2019-09-27 | 纽亘技术公司 | 用于鉴别重复测序读数的组合物和方法 |
| GB201320542D0 (en) | 2013-11-21 | 2014-01-01 | Randox Lab Ltd | Assay fluid delivery system |
| US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| CA2957633A1 (en) | 2014-08-06 | 2016-02-11 | Nugen Technologies, Inc. | Digital measurements from targeted sequencing |
| US9527077B2 (en) | 2015-01-29 | 2016-12-27 | David W. Wright | Diagnostic cartridge, fluid storage and delivery apparatus therefor and methods of construction thereof |
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| SG11201700891SA (en) | 2017-03-30 |
| US20160203259A1 (en) | 2016-07-14 |
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| CA2957633A1 (en) | 2016-02-11 |
| US20190005193A1 (en) | 2019-01-03 |
| JP2017526353A (ja) | 2017-09-14 |
| US10102337B2 (en) | 2018-10-16 |
| EP3177740A1 (en) | 2017-06-14 |
| EP3177740A4 (en) | 2018-02-28 |
| CN107075581A (zh) | 2017-08-18 |
| EP3177740B1 (en) | 2021-01-13 |
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