JP2017523774A5 - - Google Patents

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Publication number
JP2017523774A5
JP2017523774A5 JP2016575081A JP2016575081A JP2017523774A5 JP 2017523774 A5 JP2017523774 A5 JP 2017523774A5 JP 2016575081 A JP2016575081 A JP 2016575081A JP 2016575081 A JP2016575081 A JP 2016575081A JP 2017523774 A5 JP2017523774 A5 JP 2017523774A5
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JP
Japan
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nucleic acid
item
sequence
read data
acid molecules
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JP2016575081A
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Japanese (ja)
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JP2017523774A (ja
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Priority claimed from PCT/US2015/038143 external-priority patent/WO2015200871A1/en
Publication of JP2017523774A publication Critical patent/JP2017523774A/ja
Publication of JP2017523774A5 publication Critical patent/JP2017523774A5/ja
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JP2016575081A 2014-06-26 2015-06-26 サンプル分析のための方法及び組成物 Pending JP2017523774A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462017580P 2014-06-26 2014-06-26
US62/017,580 2014-06-26
US201462063870P 2014-10-14 2014-10-14
US62/063,870 2014-10-14
PCT/US2015/038143 WO2015200871A1 (en) 2014-06-26 2015-06-26 Methods and compositions for sample analysis

Publications (2)

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JP2017523774A JP2017523774A (ja) 2017-08-24
JP2017523774A5 true JP2017523774A5 (cg-RX-API-DMAC7.html) 2018-08-02

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JP2016575081A Pending JP2017523774A (ja) 2014-06-26 2015-06-26 サンプル分析のための方法及び組成物

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US (2) US20150376605A1 (cg-RX-API-DMAC7.html)
EP (1) EP3161161A4 (cg-RX-API-DMAC7.html)
JP (1) JP2017523774A (cg-RX-API-DMAC7.html)
KR (1) KR20170023011A (cg-RX-API-DMAC7.html)
CN (1) CN106574298A (cg-RX-API-DMAC7.html)
AU (1) AU2015279619A1 (cg-RX-API-DMAC7.html)
CA (1) CA2953473A1 (cg-RX-API-DMAC7.html)
IL (1) IL249618A0 (cg-RX-API-DMAC7.html)
MX (1) MX2016016898A (cg-RX-API-DMAC7.html)
WO (1) WO2015200871A1 (cg-RX-API-DMAC7.html)

Families Citing this family (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
EP2931919B1 (en) 2012-12-14 2019-02-20 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
MX2016013156A (es) 2014-04-10 2017-02-14 10X Genomics Inc Dispositivos, sistemas y metodos fluidicos, para encapsular y dividir reactivos, y aplicaciones de los mismos.
CN114214314A (zh) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 数字式pcr条码化
CA2952503A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
WO2015200869A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
BR112017008877A2 (pt) 2014-10-29 2018-07-03 10X Genomics Inc métodos e composições para sequenciamento de ácido nucleico-alvo
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
MX367432B (es) 2015-01-12 2019-08-08 10X Genomics Inc Procesos y sistemas para la preparación de bibliotecas de secuenciación de ácido nucleico y bibliotecas preparadas con estos.
CA2968417A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
EP3256606B1 (en) 2015-02-09 2019-05-22 10X Genomics, Inc. Systems and methods for determining structural variation
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
BR112017018054A2 (pt) 2015-02-24 2018-07-24 10X Genomics Inc métodos para a cobertura de sequências de ácidos nucleicos direcionadas
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
KR102723865B1 (ko) 2015-12-04 2024-10-29 10엑스 제노믹스, 인크. 핵산 분석을 위한 방법 및 조성물
JP6735348B2 (ja) 2016-02-11 2020-08-05 10エックス ジェノミクス, インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3592847B1 (en) 2017-04-26 2024-06-05 10X Genomics, Inc. Mmlv reverse transcriptase variants
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
CN117143960A (zh) 2017-05-18 2023-12-01 10X基因组学有限公司 用于分选液滴和珠的方法和系统
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
KR101979834B1 (ko) * 2017-08-11 2019-05-17 한국과학기술원 미세유체장치를 이용한 다중유전자 디지털 신호 분석장치 및 이의 분석방법
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
US10748643B2 (en) 2017-08-31 2020-08-18 10X Genomics, Inc. Systems and methods for determining the integrity of test strings with respect to a ground truth string
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019084165A1 (en) 2017-10-27 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR SAMPLE PREPARATION AND ANALYSIS
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
SG11201907566TA (en) * 2017-12-08 2019-09-27 10X Genomics Inc Methods and compositions for labeling cells
EP3724658A1 (en) 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
KR101913735B1 (ko) * 2018-05-03 2018-11-01 주식회사 셀레믹스 차세대 염기서열 분석을 위한 시료 간 교차 오염 탐색용 내부 검정 물질
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
WO2020028882A1 (en) 2018-08-03 2020-02-06 10X Genomics, Inc. Methods and systems to minimize barcode exchange
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11358137B2 (en) 2018-12-26 2022-06-14 Industrial Technology Research Institute Tubular structure for producing droplets and method for producing droplets
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
EP4073241A2 (en) 2019-12-11 2022-10-19 10X Genomics, Inc. Reverse transcriptase variants
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
WO2021163630A1 (en) 2020-02-13 2021-08-19 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and dna chromatin accessibility
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2024006392A1 (en) * 2022-06-29 2024-01-04 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US12467087B1 (en) 2024-06-25 2025-11-11 Guardant Health, Inc. Sequencing methods with partitioning

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2739484T3 (es) * 2006-02-02 2020-01-31 Univ Leland Stanford Junior Prueba genética fetal no invasiva mediante análisis digital
US20100130369A1 (en) * 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
US20120252015A1 (en) * 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
US9625454B2 (en) * 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
AU2011312218B2 (en) * 2010-10-04 2015-11-05 Sequencing Health, Inc. Systems and methods for automated reusable parallel biological reactions
EP3736281A1 (en) * 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
EP3789498B1 (en) * 2011-04-25 2025-08-13 Bio-Rad Laboratories, Inc. Methods for nucleic acid analysis
WO2013019751A1 (en) * 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
US9469874B2 (en) * 2011-10-18 2016-10-18 The Regents Of The University Of California Long-range barcode labeling-sequencing
US9176031B2 (en) * 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
WO2014028537A1 (en) * 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
WO2014074611A1 (en) * 2012-11-07 2014-05-15 Good Start Genetics, Inc. Methods and systems for identifying contamination in samples
EP2931919B1 (en) * 2012-12-14 2019-02-20 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR102436171B1 (ko) * 2013-06-27 2022-08-24 10엑스 제노믹스, 인크. 샘플 처리를 위한 조성물 및 방법

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